RchiOBHm_Chr1g0342301

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000294
Physical Location & Seq
Reverse (-)
34209318 .. 34213452
4135 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56893

Sequence Viewer

Length: 2658 bp
ATGGAGTGTCGAGGGTTGAGGCAAGTAATCATCCCGTCCTTTGTGACCTTCTGTTGCCTCATTGGTCACTTAAGTGCTAATATTGAGAAGTTAAACTATGATGTTGGTTCCATTATGGTTGAAGATCAGGTTCATGTTGGTGTAATTCTCGATATGGAATCAAAAGGAAAGATTGTTCTCAGCTGCATTTCAATGGCCCTCGCTGATTTTTACCATCTCCACAGTAACTACAGCACAAGAATAGTTCTCTACAGTAGGGATTCAAAGGTGAACCTCTGCCTGCTCTCTCTTGACTTTTTGGAAAATATCAAAGTGGAAGCAATAATTGGTGCACCAAAAAGAGCAGAAGCAAACCTTCTGGCAGAATTAGGAGAAGCGGCCAAAATCCCTTTAATGTCTCTTTCTCCTGCTCGGACTGACAATAAATATCCCTTCTTTGTTGAGATCATGCGTGCTGATGAAGCGGCTGAAGTTAAGGGAATTAGTGCCATTATTGAAGTTTTCAAATGGAGGGATGTCATCCTTTTATATGATAGCAAAGAATATGAGAGAGACTTCATTCCATCATTAGTCAAGTCATTCCAAGAGATCACACATGGGAGTGTTGCGTACAAAAGTTCCAATATTGCTTCCTCCTCATCAAATGAAGAAATCACTGCAGAGCTCCAAAAGTTGATCAACCTGAAGATTAAGGTATTTGTGGTGCATGTGTCACATTTTTTTGCACCTCGTCTTTTCTTGAATGCAAAGAAGTTAGGGATGATGAGCGAAGGGTATGCTTGGATCATGACATCAACTAGCATGAATTTCTTGCATTCTATGAATTTATCTGTTATTGAGTCAATGCAAGGAGTGCTGGGTTTCAAGTCTTCTATTCCAGCATCAATAAACCTTCACAGTCTTACATCAAGATTGAGGAGGAAGTTTTACATGGAGGATCCAAATATGGAAGCAATTCGGGAGTTAAGTGCAGATGGGATCTGGGCATATGATGCAACTTGGGCTCTAGCAGAAGCAGTTGAAAGAGCAAGGCTTAAAAATTCTACCACTAGATCCTCCAAAGACGGAGCTGTGCTTCTTAGAGAGATATTGCAGACCAGATTCAAAGGTTTAAGTGGTGAAATTCAATATGTGAATGGGAAACTAATTCCATCAGAACCATTTGAGATAGTTAATGTTATTGGAAAAGGTGAGATCAAAAGGATTGGATTTTGGCCTCGCAAAGAAGAAGTAAAATCCGGAAAAGGGTCACCCCAGCAGCAACTCATTCATAATGGGAGAAATTTAGCTTCTACAATTGATCTTGAAAGAATCATATGGCCTGGAGGTTCCAGGAGGGAATTGAGTAGTAGTGGAACACCTAAACTAAAAATTGGTGTTCCAGTGAGAATTGGGTTCAAGGAACTTGTTCGTGTCGAGCATGATCTTGAAACCAATACAATTCATGTCACTGGCTTCTCTATAGACGTATTCAAAGCTGCAATAGGAGCTTTGCCATATGAAGTGCCATACGAGTTTATTCCAATTGAGGATGCCAATGGAAATCTTGCAGGGACTTATAATGATCTTGTTTACCAGGTTTATCTCAAGAAATACGATGCTGTTGTTGGAGATGTTACGATCACAGAGAACAGATCTCAGTATGTTGATTTTACAATACCATATACCGACTTGGGTGTGGGAATGTTGGTACCAAATGCCAAGGAAAACATGTGGATTTTCTTTAAACCACTTTCAACATATCTTTGGTTAGCAAGTGCTGCTTTCTTTATCCTAACCAGTTTTGTTGTTTGGATCATTGAGCGTCCTACTAACAAAGAATTCCAGGGCACAGTAGAGGAGCAAATCGGAACACTTTTCTGGTTCTCCTTTTCTACTCTTGTGTTTGCTTATACGGAAAAGTTGTCAAATAATTTGGCAAAGTTTGTAGTGATCATATGGGTGTTTGTAGTGCTTATATTGTCATCAAGTTATACTGCAACTTTGGCATCAATGATGACGGTCAAACAGATAGAGTTAAACTCGAGAGGGAACTATATAGGTTATCAATCGGGTTCATTAGGAGTTCTAGTGAACTTAAATTTGAAAGCAGCAAAGTCATATCATTCAGAAGAAGAATATATTGATGCTCTATCCAAAGGAAGCAAGAATGGTGGGGTTTCTGGGATTATTGACGAGGTTCCGTACATCAATATTTTCCTTGCAAAGTATTCTGCTGACTACTCGATGATTAAAACCAGGGCTATCACCAATGGTTTTGCCTTTGTTTTCCCTAAAGGTTCGAAATTGGTTCACGATGTCTCAAGGCAAATTGAACGTCTGAGACAGGAAGAAAAGCTTCTAGAGATGGAAAAGACCTGGTTTCGTGAAAAAACAACTCTCATGTCTGATGAGGATATGACCCAGAGTGATCCCAATACGATTGACCTCTATGACTTTCGTGGTCTGTTCCTTATTAGTGGGGCTTCTTTAGCTATTTCTCTTTTCTTCTTCGTAGTTTTATCCACTAGATTTAGGAATCTGTTTCTAGGACTAATGCAGCTGATTCGTATAGAACTGCAGCGTTTCAGGACGTTCTTCTCCTACATAGTATGCAATGGAATTGAGCAAAACTGCATGCAAGTTCCTACGCAAGTCATTGTACATGCTGTCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

885

Amino Acids

99.86

Weight (kDa)

6.79

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 61 - 394 4.9e-54 Receptor family ligand binding region
Peripla_BP_6 PF13458 100 - 393 3.4e-10 Periplasmic binding protein
Lig_chan-Glu_bd PF10613 474 - 566 6.8e-10 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 482 - 789 2.2e-12 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 580 - 822 8.6e-27 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1560
Acc65I GGTACC 1 cut(s) 1690
AccB1I GGYRCC 1 cut(s) 1690
AccIII TCCGGA 1 cut(s) 1238
AciI CCGC 2 cut(s) 377, 464
AclWI GGATC 7 cut(s) 791, 932, 945, 986, 1047, 1802, 2405
AcoI YGGCCR 1 cut(s) 378
AcsI RAATTY 7 cut(s) 805, 823, 1039, 1122, 1282, 1820, 2080
AcuI CTGAAG 2 cut(s) 489, 704
AfaI GTAC 4 cut(s) 611, 1692, 2186, 2643
AfiI CCNNNNNNNGG 1 cut(s) 1245
AflII CTTAAG 1 cut(s) 70
AflIII ACRYGT 1 cut(s) 1710
AjnI CCWGG 6 cut(s) 1321, 1331, 1575, 1824, 2237, 2357
AjuI GAANNNNNNNTTGG 2 cut(s) 309, 341
AleI CACNNNNGTG 1 cut(s) 72
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
AluBI AGCT 9 cut(s) 183, 664, 1070, 1289, 1478, 1490, 2338, 2474, 2542
AluI AGCT 9 cut(s) 183, 664, 1070, 1289, 1478, 1490, 2338, 2474, 2542
Alw21I GWGCWC 2 cut(s) 334, 666
Alw26I GTCTC 4 cut(s) 402, 546, 2305, 2317
Alw44I GTGCAC 1 cut(s) 330
AlwI GGATC 7 cut(s) 791, 932, 945, 986, 1047, 1802, 2405
Ama87I CYCGRG 1 cut(s) 2023
Aor13HI TCCGGA 1 cut(s) 1238
AoxI GGCC 4 cut(s) 195, 378, 1214, 1319
ApaLI GTGCAC 1 cut(s) 330
ApeKI GCWGC 7 cut(s) 183, 1258, 1478, 1760, 2090, 2539, 2560
ApoI RAATTY 7 cut(s) 805, 823, 1039, 1122, 1282, 1820, 2080
ArsI GACNNNNNNTTYG 2 cut(s) 2302, 2334
Asp700I GAANNNNTTC 3 cut(s) 954, 1407, 2337
Asp718I GGTACC 1 cut(s) 1690
AspS9I GGNCC 1 cut(s) 196
AsuHPI GGTGA 5 cut(s) 280, 1130, 1202, 1242, 2239
AsuII TTCGAA 1 cut(s) 2282
AvaI CYCGRG 1 cut(s) 2023
BaeGI GKGCMC 2 cut(s) 334, 1832
BamHI GGATCC 1 cut(s) 937
BanI GGYRCC 1 cut(s) 1690
BanII GRGCYC 2 cut(s) 666, 1006
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 2 cut(s) 334, 666
BbvI GCAGC 7 cut(s) 170, 1270, 1465, 1747, 2102, 2551, 2572
BccI CCATC 5 cut(s) 222, 571, 968, 1159, 2341
BcgI CGANNNNNNTGC 4 cut(s) 758, 792, 2528, 2562
BciT130I CCWGG 6 cut(s) 1323, 1333, 1577, 1826, 2239, 2359
BclI TGATCA 2 cut(s) 675, 1932
BcoDI GTCTC 4 cut(s) 402, 546, 2305, 2317
BfaI CTAG 7 cut(s) 798, 1007, 1050, 2069, 2342, 2508, 2528
BfmI CTRYAG 5 cut(s) 229, 250, 657, 1461, 2558
BfrI CTTAAG 1 cut(s) 70
BglII AGATCT 1 cut(s) 1634
BisI GCNGC 9 cut(s) 184, 378, 465, 1259, 1479, 1761, 2091, 2540, 2561
BlsI GCNGC 9 cut(s) 185, 379, 466, 1260, 1480, 1762, 2092, 2541, 2562
Bme1390I CCNGG 6 cut(s) 1323, 1333, 1577, 1826, 2239, 2359
BmeT110I CYCGRG 1 cut(s) 2023
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 5 cut(s) 109, 939, 1330, 1692, 2181
BmrFI CCNGG 6 cut(s) 1323, 1333, 1577, 1826, 2239, 2359
BmsI GCATC 6 cut(s) 890, 982, 1522, 1588, 1997, 2116
BpiI GAAGAC 1 cut(s) 861
BplI GAGNNNNNCTC 2 cut(s) 2006, 2038
BpmI CTGGAG 1 cut(s) 1344
Bpu14I TTCGAA 1 cut(s) 2282
BpuEI CTTGAG 2 cut(s) 1571, 2287
BsaJI CCNNGG 3 cut(s) 1701, 1825, 2238
BsaWI WCCGGW 1 cut(s) 1238
BsaXI ACNNNNNCTCC 2 cut(s) 2055, 2085
Bsc4I CCNNNNNNNGG 1 cut(s) 1245
Bse1I ACTGG 3 cut(s) 1382, 1456, 1779
Bse3DI GCAATG 1 cut(s) 2602
BseAI TCCGGA 1 cut(s) 1238
BseBI CCWGG 6 cut(s) 1323, 1333, 1577, 1826, 2239, 2359
BseDI CCNNGG 3 cut(s) 1701, 1825, 2238
BseGI GGATG 5 cut(s) 30, 519, 520, 765, 1537
BseLI CCNNNNNNNGG 1 cut(s) 1245
BseMI GCAATG 1 cut(s) 2602
BseMII CTCAG 3 cut(s) 193, 1652, 2312
BseNI ACTGG 3 cut(s) 1382, 1456, 1779
BseRI GAGGAG 3 cut(s) 625, 931, 1853
BseSI GKGCMC 2 cut(s) 334, 1832
BseXI GCAGC 7 cut(s) 170, 1270, 1465, 1747, 2102, 2551, 2572
BseYI CCCAGC 2 cut(s) 856, 1254
BsgI GTGCAG 1 cut(s) 990
BshFI GGCC 4 cut(s) 197, 380, 1216, 1321
BshNI GGYRCC 1 cut(s) 1690
BsiHKAI GWGCWC 2 cut(s) 334, 666
BsiHKCI CYCGRG 1 cut(s) 2023
BsiSI CCGG 1 cut(s) 1239
BslFI GGGAC 1 cut(s) 1567
BslI CCNNNNNNNGG 1 cut(s) 1245
BsmAI GTCTC 4 cut(s) 402, 546, 2305, 2317
BsmFI GGGAC 1 cut(s) 1567
BsmI GAATGC 2 cut(s) 748, 814
BsnI GGCC 4 cut(s) 197, 380, 1216, 1321
BsoBI CYCGRG 1 cut(s) 2023
Bsp119I TTCGAA 1 cut(s) 2282
Bsp1286I GDGCHC 4 cut(s) 334, 666, 1006, 1832
Bsp13I TCCGGA 1 cut(s) 1238
Bsp1407I TGTACA 1 cut(s) 2641
BspACI CCGC 2 cut(s) 377, 464
BspANI GGCC 4 cut(s) 197, 380, 1216, 1321
BspCNI CTCAG 3 cut(s) 192, 1651, 2313
BspEI TCCGGA 1 cut(s) 1238
BspHI TCATGA 1 cut(s) 786
BspLI GGNNCC 5 cut(s) 109, 939, 1330, 1692, 2181
BspMAI CTGCAG 2 cut(s) 661, 2562
BspPI GGATC 7 cut(s) 791, 932, 945, 986, 1047, 1802, 2405
BspT104I TTCGAA 1 cut(s) 2282
BspT107I GGYRCC 1 cut(s) 1690
BspTI CTTAAG 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 2602
BsrGI TGTACA 1 cut(s) 2641
BsrI ACTGG 3 cut(s) 1382, 1456, 1779
BssECI CCNNGG 3 cut(s) 1701, 1825, 2238
BssT1I CCWWGG 1 cut(s) 1701
Bst2UI CCWGG 6 cut(s) 1323, 1333, 1577, 1826, 2239, 2359
Bst4CI ACNGT 5 cut(s) 224, 254, 899, 1834, 2002
BstAFI CTTAAG 1 cut(s) 70
BstAPI GCANNNNNTGC 3 cut(s) 853, 992, 1760
BstAUI TGTACA 1 cut(s) 2641
BstBI TTCGAA 1 cut(s) 2282
BstC8I GCNNGC 3 cut(s) 281, 453, 2618
BstDEI CTNAG 4 cut(s) 179, 1079, 1638, 2321
BstEII GGTNACC 1 cut(s) 1248
BstF5I GGATG 5 cut(s) 30, 519, 520, 765, 1537
BstMAI GTCTC 4 cut(s) 402, 546, 2305, 2317
BstMWI GCNNNNNNNGC 8 cut(s) 461, 853, 992, 1001, 1487, 1760, 1985, 2471
BstNI CCWGG 6 cut(s) 1323, 1333, 1577, 1826, 2239, 2359
BstNSI RCATGY 4 cut(s) 710, 1714, 2620, 2648
BstPI GGTNACC 1 cut(s) 1248
BstSCI CCNGG 6 cut(s) 1321, 1331, 1575, 1824, 2237, 2357
BstSFI CTRYAG 5 cut(s) 229, 250, 657, 1461, 2558
BstSLI GKGCMC 2 cut(s) 334, 1832
BstV1I GCAGC 7 cut(s) 170, 1270, 1465, 1747, 2102, 2551, 2572
BstV2I GAAGAC 1 cut(s) 861
BstX2I RGATCY 4 cut(s) 937, 978, 1052, 1634
BstYI RGATCY 4 cut(s) 937, 978, 1052, 1634
BsuRI GGCC 4 cut(s) 197, 380, 1216, 1321
BtsCI GGATG 5 cut(s) 30, 519, 520, 765, 1537
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 3 cut(s) 654, 1389, 1449
Cac8I GCNNGC 3 cut(s) 281, 453, 2618
CciI TCATGA 1 cut(s) 786
Cfr13I GGNCC 1 cut(s) 196
CseI GACGC 1 cut(s) 1793
CsiI ACCWGGT 2 cut(s) 1575, 2357
Csp6I GTAC 4 cut(s) 610, 1691, 2185, 2642
CspCI CAANNNNNGTGG 2 cut(s) 2134, 2169
CviQI GTAC 4 cut(s) 610, 1691, 2185, 2642
DdeI CTNAG 4 cut(s) 179, 1079, 1638, 2321
DraI TTTAAA 1 cut(s) 1726
EaeI YGGCCR 1 cut(s) 378
Ecl136II GAGCTC 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 1701
Eco24I GRGCYC 2 cut(s) 666, 1006
Eco53kI GAGCTC 1 cut(s) 664
Eco57I CTGAAG 2 cut(s) 489, 704
Eco88I CYCGRG 1 cut(s) 2023
Eco91I GGTNACC 1 cut(s) 1248
EcoICRI GAGCTC 1 cut(s) 664
EcoO65I GGTNACC 1 cut(s) 1248
EcoRI GAATTC 1 cut(s) 1820
EcoRII CCWGG 6 cut(s) 1321, 1331, 1575, 1824, 2237, 2357
EcoT14I CCWWGG 1 cut(s) 1701
EcoT38I GRGCYC 2 cut(s) 666, 1006
ErhI CCWWGG 1 cut(s) 1701
FalI AAGNNNNNCTT 6 cut(s) 339, 371, 1747, 1779, 2322, 2354
FaqI GGGAC 1 cut(s) 1567
FauNDI CATATG 4 cut(s) 988, 1316, 1498, 1937
FbaI TGATCA 2 cut(s) 675, 1932
Fnu4HI GCNGC 9 cut(s) 184, 378, 465, 1259, 1479, 1761, 2091, 2540, 2561
FokI GGATG 5 cut(s) 17, 506, 527, 772, 1544
FriOI GRGCYC 2 cut(s) 666, 1006
Fsp4HI GCNGC 9 cut(s) 184, 378, 465, 1259, 1479, 1761, 2091, 2540, 2561
FspBI CTAG 7 cut(s) 798, 1007, 1050, 2069, 2342, 2508, 2528
GluI GCNGC 9 cut(s) 184, 378, 465, 1259, 1479, 1761, 2091, 2540, 2561
GsaI CCCAGC 2 cut(s) 860, 1258
GsuI CTGGAG 1 cut(s) 1344
HaeIII GGCC 4 cut(s) 197, 380, 1216, 1321
HapII CCGG 1 cut(s) 1239
HgaI GACGC 1 cut(s) 1793
HindIII AAGCTT 1 cut(s) 2336
HinfI GANTC 7 cut(s) 158, 260, 839, 1101, 1311, 2518, 2545
HpaII CCGG 1 cut(s) 1239
HphI GGTGA 5 cut(s) 280, 1130, 1202, 1242, 2239
Hpy166II GTNNAC 5 cut(s) 271, 332, 1573, 2074, 2293
Hpy188I TCNGA 6 cut(s) 414, 1156, 1850, 2110, 2322, 2389
Hpy8I GTNNAC 5 cut(s) 271, 332, 1573, 2074, 2293
HpyAV CCTTC 5 cut(s) 58, 365, 442, 764, 902
HpyCH4III ACNGT 5 cut(s) 224, 254, 899, 1834, 2002
HpyCH4IV ACGT 3 cut(s) 1467, 2317, 2573
HpyF10VI GCNNNNNNNGC 8 cut(s) 461, 853, 992, 1001, 1487, 1760, 1985, 2471
HpyF3I CTNAG 4 cut(s) 179, 1079, 1638, 2321
HpySE526I ACGT 3 cut(s) 1467, 2317, 2573
Kpn2I TCCGGA 1 cut(s) 1238
KpnI GGTACC 1 cut(s) 1694
Ksp22I TGATCA 2 cut(s) 675, 1932
LmnI GCTCC 4 cut(s) 669, 1067, 1487, 1840
Lsp1109I GCAGC 7 cut(s) 170, 1270, 1465, 1747, 2102, 2551, 2572
LweI GCATC 6 cut(s) 890, 982, 1522, 1588, 1997, 2116
MabI ACCWGGT 2 cut(s) 1575, 2357
MaeI CTAG 7 cut(s) 798, 1007, 1050, 2069, 2342, 2508, 2528
MaeII ACGT 3 cut(s) 1467, 2317, 2573
MaeIII GTNAC 7 cut(s) 43, 65, 224, 711, 1248, 1447, 1615
MfeI CAATTG 2 cut(s) 1296, 1524
MflI RGATCY 4 cut(s) 937, 978, 1052, 1634
MhlI GDGCHC 4 cut(s) 334, 666, 1006, 1832
MlyI GAGTC 1 cut(s) 848
MmeI TCCRAC 1 cut(s) 1588
MroI TCCGGA 1 cut(s) 1238
MroXI GAANNNNTTC 3 cut(s) 954, 1407, 2337
MslI CAYNNNNRTG 5 cut(s) 72, 138, 191, 600, 1940
MspA1I CMGCKG 2 cut(s) 183, 2542
MspCI CTTAAG 1 cut(s) 70
MspI CCGG 1 cut(s) 1239
MspR9I CCNGG 6 cut(s) 1323, 1333, 1577, 1826, 2239, 2359
MunI CAATTG 2 cut(s) 1296, 1524
Mva1269I GAATGC 2 cut(s) 748, 814
MvaI CCWGG 6 cut(s) 1323, 1333, 1577, 1826, 2239, 2359
MwoI GCNNNNNNNGC 8 cut(s) 461, 853, 992, 1001, 1487, 1760, 1985, 2471
NdeI CATATG 4 cut(s) 988, 1316, 1498, 1937
NlaIV GGNNCC 5 cut(s) 109, 939, 1330, 1692, 2181
NmuCI GTSAC 5 cut(s) 43, 65, 711, 1248, 1447
NspI RCATGY 4 cut(s) 710, 1714, 2620, 2648
NspV TTCGAA 1 cut(s) 2282
OliI CACNNNNGTG 1 cut(s) 72
PaeI GCATGC 1 cut(s) 2620
PaeR7I CTCGAG 1 cut(s) 2023
PagI TCATGA 1 cut(s) 786
PciI ACATGT 1 cut(s) 1710
PctI GAATGC 2 cut(s) 748, 814
PdmI GAANNNNTTC 3 cut(s) 954, 1407, 2337
PfeI GAWTC 6 cut(s) 158, 260, 1101, 1311, 2518, 2545
PfoI TCCNGGA 1 cut(s) 1331
PkrI GCNGC 9 cut(s) 185, 379, 466, 1260, 1480, 1762, 2092, 2541, 2562
PleI GAGTC 1 cut(s) 847
PpsI GAGTC 1 cut(s) 847
PscI ACATGT 1 cut(s) 1710
PsiI TTATAA 1 cut(s) 1560
Psp124BI GAGCTC 1 cut(s) 666
Psp6I CCWGG 6 cut(s) 1321, 1331, 1575, 1824, 2237, 2357
PspEI GGTNACC 1 cut(s) 1248
PspFI CCCAGC 2 cut(s) 856, 1254
PspGI CCWGG 6 cut(s) 1321, 1331, 1575, 1824, 2237, 2357
PspN4I GGNNCC 5 cut(s) 109, 939, 1330, 1692, 2181
PspPI GGNCC 1 cut(s) 196
PstI CTGCAG 2 cut(s) 661, 2562
PsuI RGATCY 4 cut(s) 937, 978, 1052, 1634
PvuII CAGCTG 2 cut(s) 183, 2542
RsaI GTAC 4 cut(s) 611, 1692, 2186, 2643
RsaNI GTAC 4 cut(s) 610, 1691, 2185, 2642
RseI CAYNNNNRTG 5 cut(s) 72, 138, 191, 600, 1940
SacI GAGCTC 1 cut(s) 666
SatI GCNGC 9 cut(s) 184, 378, 465, 1259, 1479, 1761, 2091, 2540, 2561
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 848
ScrFI CCNGG 6 cut(s) 1323, 1333, 1577, 1826, 2239, 2359
SduI GDGCHC 4 cut(s) 334, 666, 1006, 1832
SexAI ACCWGGT 2 cut(s) 1575, 2357
SfaNI GCATC 6 cut(s) 890, 982, 1522, 1588, 1997, 2116
SfcI CTRYAG 5 cut(s) 229, 250, 657, 1461, 2558
Sfr274I CTCGAG 1 cut(s) 2023
SfuI TTCGAA 1 cut(s) 2282
SlaI CTCGAG 1 cut(s) 2023
SmiMI CAYNNNNRTG 5 cut(s) 72, 138, 191, 600, 1940
SmlI CTYRAG 4 cut(s) 70, 1586, 2023, 2302
SmoI CTYRAG 4 cut(s) 70, 1586, 2023, 2302
SphI GCATGC 1 cut(s) 2620
SsiI CCGC 2 cut(s) 377, 464
SspI AATATT 3 cut(s) 82, 625, 2194
SspMI CTAG 7 cut(s) 798, 1007, 1050, 2069, 2342, 2508, 2528
SstI GAGCTC 1 cut(s) 666
StyD4I CCNGG 6 cut(s) 1321, 1331, 1575, 1824, 2237, 2357
StyI CCWWGG 1 cut(s) 1701
TaaI ACNGT 5 cut(s) 224, 254, 899, 1834, 2002
TaiI ACGT 3 cut(s) 1470, 2320, 2576
TaqI TCGA 6 cut(s) 10, 150, 1416, 2024, 2225, 2282
TatI WGTACW 1 cut(s) 2641
TauI GCSGC 2 cut(s) 380, 467
TfiI GAWTC 6 cut(s) 158, 260, 1101, 1311, 2518, 2545
TscAI CASTG 3 cut(s) 661, 1389, 1456
TseFI GTSAC 5 cut(s) 43, 65, 711, 1248, 1447
TseI GCWGC 7 cut(s) 183, 1258, 1478, 1760, 2090, 2539, 2560
Tsp45I GTSAC 5 cut(s) 43, 65, 711, 1248, 1447
TspGWI ACGGA 3 cut(s) 1080, 1910, 2172
TspRI CASTG 3 cut(s) 661, 1389, 1456
Vha464I CTTAAG 1 cut(s) 70
VneI GTGCAC 1 cut(s) 330
XapI RAATTY 7 cut(s) 805, 823, 1039, 1122, 1282, 1820, 2080
XbaI TCTAGA 1 cut(s) 2341
XceI RCATGY 4 cut(s) 710, 1714, 2620, 2648
XhoI CTCGAG 1 cut(s) 2023
XmnI GAANNNNTTC 3 cut(s) 954, 1407, 2337
XspI CTAG 7 cut(s) 798, 1007, 1050, 2069, 2342, 2508, 2528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.