Rw1G014150

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000294
Physical Location & Seq
Reverse (-)
31544263 .. 31549297
5035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G014150.1

Sequence Viewer

Length: 2598 bp
ATGGCTCAAATGTATGGAATTTCACTCTGCATATTATTTTGCTTCCTGGGTCTCTTGTGTGCTGAAGTTGATGAAAAAGTGCATTACAGCAGTCCTAGTGTTGTGGACGAGGTTGACGTTGGTGTGATTCTTGATATGGGATCAACGGAAGCAAAACTTGTTCTCAGTTGCATTTCAATGGCCCTGTCTGATTTCTACAATCTGCACAACAACTACAGCACAAGAGTAGTTCTCCACAGTAGGGATTCCCATGGAAAAGCTCTACATGCTCTATCAGCTGCCCTTAGTCTGTTGGATAACATCAAAGTGGAAGCAATAATTAGTGCAGAACCAAGAATGGAAGCAGACCTATTGGAAGAATTAGGAGGAGTTGAAGTCCCAGTATTGTCTCTGTCTCAACCTATTACTTCACCTCCTCTGACTCATAGATATCCCTTCTTCATTGAGATCAGACAGGATGAAACTTCTCAGGTTATGGGAATTGTTTCCCTTATTAAAACGTACAAATGGAAGGATGTTATCCTTCGATATGAGAGCACAAAATATGCATGCGACATCATTCCATCTTTGATTAGTTCCTTCCAAGAGAAAAATGTCTATGTTTCATATAAGAGTGCCATATCTGCCTCCCCAACAAATCAACAAATCATTGAGGAGCTCCACAAGCTCATGACATTGAAGACTAGGATAATTGTGGTGCATATGTCCCATGTGCTCATGCCTCGCCTATTCTTAAATGCAAAAAAGTTAGGAATGATGAGAGAAGGTTATGCCTGGATTATGACATCAACTAGTATGAATTTCTTGCAGTCCATGGGCTTATCTGTCATTGAGTCTATGCAAGGAGTGCTGGGTTTCAAGTCTTATATTCCAGCTTCAACCAACCTCTACAAACTTACTTCCAGAATCAGAAGAAAATTTTACATTGAGGAGCCTGATATGGAACTAGGTAGGGAGTTAAGTGCAGATGGAATCTGGGCATATGATGCAACTTGGGCTCTATCAGAAGCAGTTGAGAGGACAAGAATCAAAAGTTCTACTATTGGATCCTCCAAGCATGGAATTGAGCTTCTTAGAGAGATTCTGCAAACTAGATTTAAAGGTTTAAGTGGCGAAGTTCAATATCCAAATGGGAACTTGATTTCAAGTGGATTTGAGATAGTGAACGTCATCGGAACAGGGGTGAGAAGAGTTGGATTTTGGCCTTGTGAAGAAGAAAAAATCACAAAAGAGACACTCCCAGGTAGTAACAGGAGGAATGGACTTTCCACAAACCATCTGGAAACAATCATATGGCCTGGAGGATCATCAACCATCACGAGACGTTCCAAGAGGCGACTGAGTGGAATTAAACTAAGAGTTGGGGTTCCAGTAAAAATAGGCTTCAAGGAACTTGTACGTGTGGAGCATGATCTTCAAACCAATAAAACTCATGTTACGGGCTTCTGTATAGATGTATTCGAAGCTGCAATTAGAGGATTGCCTTATAAAGTACAGTATGAGTTTATTCCATTTGAGGATGCCAATGGAAAGTCAGCTGGGAAGTACAATGATCTAGTTTACAACGTTTATCTAAAGAAATACGATGCTGTTGCTGGAGATACGACGATCACATCGAATAGATCTTTGTATGTTGATTTTACAATACCATTTACTGACATGGGGGTGGGAATGTTAGTACCAAATGAAAAGGAAAACATGTGGGTTTTCTTAGAACCTCTGTCAGCAAATCTTTGGGTGACAAGTGGTGGCTTTTTTATCCTAACGGGTTTTGTTGTGTGGATAATTGAGCGTCCTGTCAACCAAGAGTTCCAGGGATCAACATCACAGCAACTCGGAACAATATTCTGGTTCTCCTTCTCAACCCTTGTGTTTGCTCATAGGGAAAAGTTGTTGAACAACTTGGCCAAGTTTGTCACGATCGTCTGGGTTTTTGTAGTGCTTATATTGACATCCAGTTATACTGCAACTTTAGCATCAATGCTGACAGTTAAACAGATAAATTTAAACTCAAGAGGTAAATATGTAGCTGACCTTGCGATTTTTGGAGGGCCGAAGCAAAAGTTGTATCATACAGTTGAACAATTTGCTGATGCTTTATCAAGTGGAAGTAAGAATGGTGGTGTTTCTGCTATCATCGAAGAGATTCCATACCTCAAAATCTTCCTAGCAAAGTATCCTGTTGACTACTCCATGATTAAACCCAAGTTAACTACCAATGGTTTTGGCTTTGTTTTTCCTAAAGGTTCTAAATTGGTCCATGACATGTCGAGGCAAATTGAAATTTTAAGAGAAGAAGGAAAGCTTTTAGAGATGGAGAAGGCCTGGTTTCAGATATCAGATGCAGTGAATAGTCCTAATCCCGGTACTCTTAACCTCAGCAGCTTTCTAGGTTTATTCCTTGTTAGTGGCGTTTGTTCAGCTGCTGCTCTTATATTATTCTTCATTTTGTCCCTTGAAGATGGATGGCATGTCGTAAAGATAAGCACAGCTAGATCCTTGAGTAGAGGACAGCTGATGCAAGTTATAAGAAGGTACCTAACTAACAAACTATCAAATGCAGATGAACACATCAATACACAGATAGTGGATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan Lig_chan-Glu_bd SBP_bac_3

Protein Analysis

865

Amino Acids

96.87

Weight (kDa)

8.34

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 38 - 374 1.6e-07 Periplasmic binding protein
ANF_receptor PF01094 55 - 392 1.1e-62 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 470 - 562 3.2e-10 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 474 - 778 1.7e-10 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 576 - 805 2.5e-28 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1488, 2528
Acc65I GGTACC 1 cut(s) 2535
AccB1I GGYRCC 1 cut(s) 2535
AclI AACGTT 1 cut(s) 1566
AclWI GGATC 6 cut(s) 148, 1041, 1054, 1312, 1825, 2490
AcoI YGGCCR 1 cut(s) 1905
AcsI RAATTY 5 cut(s) 18, 799, 917, 2002, 2283
AcuI CTGAAG 1 cut(s) 84
AfaI GTAC 7 cut(s) 503, 1398, 1494, 1547, 1680, 2368, 2537
AfiI CCNNNNNNNGG 2 cut(s) 241, 2363
AflIII ACRYGT 3 cut(s) 1399, 1698, 2265
AhlI ACTAGT 1 cut(s) 791
AjnI CCWGG 6 cut(s) 45, 773, 1240, 1297, 1812, 2324
Alw21I GWGCWC 3 cut(s) 539, 660, 717
Alw26I GTCTC 5 cut(s) 56, 393, 399, 1226, 1315
AlwI GGATC 6 cut(s) 148, 1041, 1054, 1312, 1825, 2490
AlwNI CAGNNNCTG 1 cut(s) 2426
AoxI GGCC 6 cut(s) 180, 1202, 1295, 1905, 2051, 2322
ApeKI GCWGC 5 cut(s) 278, 1466, 2382, 2423, 2426
ApoI RAATTY 5 cut(s) 18, 799, 917, 2002, 2283
Asp700I GAANNNNTTC 2 cut(s) 484, 2145
Asp718I GGTACC 1 cut(s) 2535
AspS9I GGNCC 3 cut(s) 181, 2051, 2257
AsuC2I CCSGG 1 cut(s) 2364
AsuHPI GGTGA 3 cut(s) 402, 1195, 1750
AsuII TTCGAA 1 cut(s) 1461
AvaII GGWCC 1 cut(s) 2257
BalI TGGCCA 1 cut(s) 1907
BamHI GGATCC 1 cut(s) 1046
BanI GGYRCC 1 cut(s) 2535
BanII GRGCYC 2 cut(s) 660, 1000
BauI CACGAG 1 cut(s) 1318
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 3 cut(s) 539, 660, 717
BbvCI CCTCAGC 1 cut(s) 2378
BbvI GCAGC 5 cut(s) 265, 1453, 2394, 2410, 2413
BccI CCATC 7 cut(s) 571, 962, 1284, 1322, 2308, 2456, 2460
BcgI CGANNNNNNTGC 2 cut(s) 1574, 1608
BciT130I CCWGG 6 cut(s) 47, 775, 1242, 1299, 1814, 2326
BciVI GTATCC 1 cut(s) 2187
BcnI CCSGG 1 cut(s) 2364
BcoDI GTCTC 5 cut(s) 56, 393, 399, 1226, 1315
BcuI ACTAGT 1 cut(s) 791
BfaI CTAG 9 cut(s) 96, 684, 792, 947, 1092, 1556, 2168, 2390, 2493
BfmI CTRYAG 1 cut(s) 214
BfuI GTATCC 1 cut(s) 2187
BglII AGATCT 1 cut(s) 1622
BisI GCNGC 5 cut(s) 279, 1467, 2383, 2424, 2427
BlsI GCNGC 5 cut(s) 280, 1468, 2384, 2425, 2428
Bme1390I CCNGG 7 cut(s) 47, 775, 1242, 1299, 1814, 2326, 2364
Bme18I GGWCC 1 cut(s) 2257
BmgT120I GGNCC 3 cut(s) 181, 2051, 2257
BmiI GGNNCC 4 cut(s) 933, 1048, 1368, 2537
BmrFI CCNGG 7 cut(s) 47, 775, 1242, 1299, 1814, 2326, 2364
BmrI ACTGGG 1 cut(s) 374
BmsI GCATC 7 cut(s) 976, 1510, 1576, 1985, 2083, 2332, 2508
BmuI ACTGGG 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 686
BplI GAGNNNNNCTC 2 cut(s) 216, 248
BpmI CTGGAG 2 cut(s) 1320, 1617
Bpu10I CCTNAGC 1 cut(s) 2378
Bpu14I TTCGAA 1 cut(s) 1461
BpuEI CTTGAG 2 cut(s) 1996, 2521
BpuMI CCSGG 1 cut(s) 2364
BsaAI YACGTR 1 cut(s) 1400
BsaBI GATNNNNATC 1 cut(s) 1822
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 5 cut(s) 46, 250, 813, 1240, 1813
BsaXI ACNNNNNCTCC 4 cut(s) 360, 390, 397, 427
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 2363
Bse1I ACTGG 3 cut(s) 380, 1370, 1956
Bse8I GATNNNNATC 1 cut(s) 1822
BseBI CCWGG 6 cut(s) 47, 775, 1242, 1299, 1814, 2326
BseDI CCNNGG 5 cut(s) 46, 250, 813, 1240, 1813
BseGI GGATG 5 cut(s) 463, 520, 1525, 1952, 2471
BseJI GATNNNNATC 1 cut(s) 1822
BseLI CCNNNNNNNGG 2 cut(s) 241, 2363
BseMII CTCAG 4 cut(s) 178, 482, 1331, 2392
BseNI ACTGG 3 cut(s) 380, 1370, 1956
BseRI GAGGAG 4 cut(s) 381, 405, 668, 944
BseXI GCAGC 5 cut(s) 265, 1453, 2394, 2410, 2413
BseYI CCCAGC 2 cut(s) 850, 1538
BsgI GTGCAG 3 cut(s) 188, 345, 984
Bsh1285I CGRYCG 1 cut(s) 1923
BshFI GGCC 6 cut(s) 182, 1204, 1297, 1907, 2053, 2324
BshNI GGYRCC 1 cut(s) 2535
BsiEI CGRYCG 1 cut(s) 1923
BsiHKAI GWGCWC 3 cut(s) 539, 660, 717
BsiSI CCGG 1 cut(s) 2364
BslFI GGGAC 3 cut(s) 362, 691, 2437
BslI CCNNNNNNNGG 2 cut(s) 241, 2363
BsmAI GTCTC 5 cut(s) 56, 393, 399, 1226, 1315
BsmBI CGTCTC 1 cut(s) 1315
BsmFI GGGAC 3 cut(s) 362, 691, 2437
BsnI GGCC 6 cut(s) 182, 1204, 1297, 1907, 2053, 2324
Bso31I GGTCTC 1 cut(s) 56
Bsp119I TTCGAA 1 cut(s) 1461
Bsp1286I GDGCHC 4 cut(s) 539, 660, 717, 1000
Bsp19I CCATGG 2 cut(s) 250, 813
BspANI GGCC 6 cut(s) 182, 1204, 1297, 1907, 2053, 2324
BspCNI CTCAG 4 cut(s) 177, 481, 1332, 2391
BspHI TCATGA 1 cut(s) 669
BspLI GGNNCC 4 cut(s) 933, 1048, 1368, 2537
BspPI GGATC 6 cut(s) 148, 1041, 1054, 1312, 1825, 2490
BspT104I TTCGAA 1 cut(s) 1461
BspT107I GGYRCC 1 cut(s) 2535
BspTNI GGTCTC 1 cut(s) 56
BsrI ACTGG 3 cut(s) 380, 1370, 1956
BssECI CCNNGG 5 cut(s) 46, 250, 813, 1240, 1813
BssSI CACGAG 1 cut(s) 1318
BssT1I CCWWGG 2 cut(s) 250, 813
Bst2BI CACGAG 1 cut(s) 1318
Bst2UI CCWGG 6 cut(s) 47, 775, 1242, 1299, 1814, 2326
Bst4CI ACNGT 4 cut(s) 239, 1497, 1990, 2077
Bst6I CTCTTC 2 cut(s) 1183, 2136
BstAPI GCANNNNNTGC 2 cut(s) 847, 986
BstBAI YACGTR 1 cut(s) 1400
BstBI TTCGAA 1 cut(s) 1461
BstC8I GCNNGC 1 cut(s) 550
BstDEI CTNAG 8 cut(s) 164, 284, 468, 1073, 1340, 1355, 1711, 2378
BstDSI CCRYGG 2 cut(s) 250, 813
BstF5I GGATG 5 cut(s) 463, 520, 1525, 1952, 2471
BstMAI GTCTC 5 cut(s) 56, 393, 399, 1226, 1315
BstMCI CGRYCG 1 cut(s) 1923
BstMWI GCNNNNNNNGC 9 cut(s) 266, 275, 623, 664, 847, 986, 995, 1973, 2036
BstNI CCWGG 6 cut(s) 47, 775, 1242, 1299, 1814, 2326
BstNSI RCATGY 5 cut(s) 269, 552, 1702, 2269, 2474
BstSCI CCNGG 7 cut(s) 45, 773, 1240, 1297, 1812, 2324, 2362
BstSFI CTRYAG 1 cut(s) 214
BstV1I GCAGC 5 cut(s) 265, 1453, 2394, 2410, 2413
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 3 cut(s) 1046, 1622, 2495
BstYI RGATCY 3 cut(s) 1046, 1622, 2495
BsuI GTATCC 1 cut(s) 2187
BsuRI GGCC 6 cut(s) 182, 1204, 1297, 1907, 2053, 2324
BtgI CCRYGG 2 cut(s) 250, 813
BtsCI GGATG 5 cut(s) 463, 520, 1525, 1952, 2471
BtsI GCAGTG 1 cut(s) 2352
BtsIMutI CAGTG 1 cut(s) 2352
Cac8I GCNNGC 1 cut(s) 550
CaiI CAGNNNCTG 1 cut(s) 2426
CciI TCATGA 1 cut(s) 669
Cfr13I GGNCC 3 cut(s) 181, 2051, 2257
CseI GACGC 1 cut(s) 1781
Csp6I GTAC 7 cut(s) 502, 1397, 1493, 1546, 1679, 2367, 2536
CviQI GTAC 7 cut(s) 502, 1397, 1493, 1546, 1679, 2367, 2536
DdeI CTNAG 8 cut(s) 164, 284, 468, 1073, 1340, 1355, 1711, 2378
DraI TTTAAA 2 cut(s) 1099, 2007
EaeI YGGCCR 1 cut(s) 1905
Eam1104I CTCTTC 2 cut(s) 1183, 2136
EarI CTCTTC 2 cut(s) 1183, 2136
Ecl136II GAGCTC 1 cut(s) 658
Eco130I CCWWGG 2 cut(s) 250, 813
Eco147I AGGCCT 1 cut(s) 2324
Eco24I GRGCYC 2 cut(s) 660, 1000
Eco31I GGTCTC 1 cut(s) 56
Eco32I GATATC 2 cut(s) 431, 2337
Eco47I GGWCC 1 cut(s) 2257
Eco53kI GAGCTC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 84
EcoICRI GAGCTC 1 cut(s) 658
EcoRII CCWGG 6 cut(s) 45, 773, 1240, 1297, 1812, 2324
EcoRV GATATC 2 cut(s) 431, 2337
EcoT14I CCWWGG 2 cut(s) 250, 813
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 2 cut(s) 660, 1000
ErhI CCWWGG 2 cut(s) 250, 813
Esp3I CGTCTC 1 cut(s) 1315
FalI AAGNNNNNCTT 4 cut(s) 141, 173, 2289, 2321
FaqI GGGAC 3 cut(s) 362, 691, 2437
FauNDI CATATG 3 cut(s) 702, 982, 1292
Fnu4HI GCNGC 5 cut(s) 279, 1467, 2383, 2424, 2427
FokI GGATG 5 cut(s) 470, 527, 1532, 1939, 2478
FriOI GRGCYC 2 cut(s) 660, 1000
Fsp4HI GCNGC 5 cut(s) 279, 1467, 2383, 2424, 2427
FspBI CTAG 9 cut(s) 96, 684, 792, 947, 1092, 1556, 2168, 2390, 2493
GluI GCNGC 5 cut(s) 279, 1467, 2383, 2424, 2427
GsaI CCCAGC 2 cut(s) 854, 1542
GsuI CTGGAG 2 cut(s) 1320, 1617
HaeIII GGCC 6 cut(s) 182, 1204, 1297, 1907, 2053, 2324
HapII CCGG 1 cut(s) 2364
HgaI GACGC 1 cut(s) 1781
HincII GTYRAC 4 cut(s) 115, 1801, 2185, 2211
HindII GTYRAC 4 cut(s) 115, 1801, 2185, 2211
HindIII AAGCTT 1 cut(s) 2303
HpaI GTTAAC 1 cut(s) 2211
HpaII CCGG 1 cut(s) 2364
HphI GGTGA 3 cut(s) 402, 1195, 1750
Hpy166II GTNNAC 7 cut(s) 106, 115, 1165, 1561, 1801, 2185, 2211
Hpy188I TCNGA 9 cut(s) 190, 420, 452, 911, 1006, 1175, 1838, 2334, 2341
Hpy188III TCNNGA 7 cut(s) 131, 670, 903, 1280, 1318, 1918, 2013
Hpy8I GTNNAC 7 cut(s) 106, 115, 1165, 1561, 1801, 2185, 2211
Hpy99I CGWCG 1 cut(s) 1609
HpyAV CCTTC 9 cut(s) 445, 505, 533, 589, 758, 1867, 2291, 2314, 2526
HpyCH4III ACNGT 4 cut(s) 239, 1497, 1990, 2077
HpyCH4IV ACGT 6 cut(s) 117, 500, 1167, 1324, 1399, 1566
HpyF10VI GCNNNNNNNGC 9 cut(s) 266, 275, 623, 664, 847, 986, 995, 1973, 2036
HpyF3I CTNAG 8 cut(s) 164, 284, 468, 1073, 1340, 1355, 1711, 2378
HpySE526I ACGT 6 cut(s) 117, 500, 1167, 1324, 1399, 1566
KpnI GGTACC 1 cut(s) 2539
KspAI GTTAAC 1 cut(s) 2211
LmnI GCTCC 4 cut(s) 655, 663, 931, 1405
Lsp1109I GCAGC 5 cut(s) 265, 1453, 2394, 2410, 2413
LweI GCATC 7 cut(s) 976, 1510, 1576, 1985, 2083, 2332, 2508
MaeI CTAG 9 cut(s) 96, 684, 792, 947, 1092, 1556, 2168, 2390, 2493
MaeII ACGT 6 cut(s) 117, 500, 1167, 1324, 1399, 1566
MaeIII GTNAC 4 cut(s) 1247, 1435, 1738, 1915
MflI RGATCY 3 cut(s) 1046, 1622, 2495
MhlI GDGCHC 4 cut(s) 539, 660, 717, 1000
MlsI TGGCCA 1 cut(s) 1907
MluNI TGGCCA 1 cut(s) 1907
MlyI GAGTC 2 cut(s) 415, 842
MmeI TCCRAC 2 cut(s) 273, 1174
Mox20I TGGCCA 1 cut(s) 1907
Mph1103I ATGCAT 1 cut(s) 550
MroXI GAANNNNTTC 2 cut(s) 484, 2145
MscI TGGCCA 1 cut(s) 1907
MslI CAYNNNNRTG 3 cut(s) 176, 305, 1664
Msp20I TGGCCA 1 cut(s) 1907
MspA1I CMGCKG 4 cut(s) 278, 1538, 2423, 2515
MspI CCGG 1 cut(s) 2364
MspR9I CCNGG 7 cut(s) 47, 775, 1242, 1299, 1814, 2326, 2364
MvaI CCWGG 6 cut(s) 47, 775, 1242, 1299, 1814, 2326
MwoI GCNNNNNNNGC 9 cut(s) 266, 275, 623, 664, 847, 986, 995, 1973, 2036
NciI CCSGG 1 cut(s) 2364
NcoI CCATGG 2 cut(s) 250, 813
NdeI CATATG 3 cut(s) 702, 982, 1292
NlaIV GGNNCC 4 cut(s) 933, 1048, 1368, 2537
NmuCI GTSAC 2 cut(s) 1738, 1915
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 5 cut(s) 269, 552, 1702, 2269, 2474
NspV TTCGAA 1 cut(s) 1461
PaeI GCATGC 1 cut(s) 552
PagI TCATGA 1 cut(s) 669
PceI AGGCCT 1 cut(s) 2324
PciI ACATGT 2 cut(s) 1698, 2265
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 1613
PdmI GAANNNNTTC 2 cut(s) 484, 2145
PfeI GAWTC 8 cut(s) 127, 245, 906, 972, 1026, 1081, 2146, 2591
PkrI GCNGC 5 cut(s) 280, 1468, 2384, 2425, 2428
Ple19I CGATCG 1 cut(s) 1923
PleI GAGTC 2 cut(s) 415, 841
PpsI GAGTC 2 cut(s) 415, 841
Ppu21I YACGTR 1 cut(s) 1400
PscI ACATGT 2 cut(s) 1698, 2265
PsiI TTATAA 2 cut(s) 1488, 2528
Psp124BI GAGCTC 1 cut(s) 660
Psp1406I AACGTT 1 cut(s) 1566
Psp6I CCWGG 6 cut(s) 45, 773, 1240, 1297, 1812, 2324
PspFI CCCAGC 2 cut(s) 850, 1538
PspGI CCWGG 6 cut(s) 45, 773, 1240, 1297, 1812, 2324
PspN4I GGNNCC 4 cut(s) 933, 1048, 1368, 2537
PspPI GGNCC 3 cut(s) 181, 2051, 2257
PstNI CAGNNNCTG 1 cut(s) 2426
PsuI RGATCY 3 cut(s) 1046, 1622, 2495
PvuI CGATCG 1 cut(s) 1923
PvuII CAGCTG 4 cut(s) 278, 1538, 2423, 2515
RsaI GTAC 7 cut(s) 503, 1398, 1494, 1547, 1680, 2368, 2537
RsaNI GTAC 7 cut(s) 502, 1397, 1493, 1546, 1679, 2367, 2536
RseI CAYNNNNRTG 3 cut(s) 176, 305, 1664
SacI GAGCTC 1 cut(s) 660
SatI GCNGC 5 cut(s) 279, 1467, 2383, 2424, 2427
Sau96I GGNCC 3 cut(s) 181, 2051, 2257
SchI GAGTC 2 cut(s) 415, 842
ScrFI CCNGG 7 cut(s) 47, 775, 1242, 1299, 1814, 2326, 2364
SduI GDGCHC 4 cut(s) 539, 660, 717, 1000
SfaNI GCATC 7 cut(s) 976, 1510, 1576, 1985, 2083, 2332, 2508
SfcI CTRYAG 1 cut(s) 214
SfuI TTCGAA 1 cut(s) 1461
SinI GGWCC 1 cut(s) 2257
SmiMI CAYNNNNRTG 3 cut(s) 176, 305, 1664
SmlI CTYRAG 2 cut(s) 2011, 2500
SmoI CTYRAG 2 cut(s) 2011, 2500
SpeI ACTAGT 1 cut(s) 791
SphI GCATGC 1 cut(s) 552
SseBI AGGCCT 1 cut(s) 2324
SspI AATATT 1 cut(s) 1845
SspMI CTAG 9 cut(s) 96, 684, 792, 947, 1092, 1556, 2168, 2390, 2493
SstI GAGCTC 1 cut(s) 660
StuI AGGCCT 1 cut(s) 2324
StyD4I CCNGG 7 cut(s) 45, 773, 1240, 1297, 1812, 2324, 2362
StyI CCWWGG 2 cut(s) 250, 813
TaaI ACNGT 4 cut(s) 239, 1497, 1990, 2077
TaiI ACGT 6 cut(s) 120, 503, 1170, 1327, 1402, 1569
TaqI TCGA 5 cut(s) 526, 1461, 1616, 2139, 2270
TatI WGTACW 2 cut(s) 1492, 1545
TfiI GAWTC 8 cut(s) 127, 245, 906, 972, 1026, 1081, 2146, 2591
TscAI CASTG 1 cut(s) 2352
TseFI GTSAC 2 cut(s) 1738, 1915
TseI GCWGC 5 cut(s) 278, 1466, 2382, 2423, 2426
Tsp45I GTSAC 2 cut(s) 1738, 1915
TspDTI ATGAA 9 cut(s) 87, 430, 474, 594, 812, 1701, 2434, 2580, 2583
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 1 cut(s) 2352
VpaK11BI GGWCC 1 cut(s) 2257
XapI RAATTY 5 cut(s) 18, 799, 917, 2002, 2283
XceI RCATGY 5 cut(s) 269, 552, 1702, 2269, 2474
XcmI CCANNNNNNNNNTGG 1 cut(s) 1276
XmnI GAANNNNTTC 2 cut(s) 484, 2145
XspI CTAG 9 cut(s) 96, 684, 792, 947, 1092, 1556, 2168, 2390, 2493
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.