Rroxscaffold_4G00312540

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000294
Physical Location & Seq
Reverse (-)
35551553 .. 35557696
6144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312540.1

Sequence Viewer

Length: 2601 bp
ATGGAGTTTCAAGGGTTGAGGCAAACAGTCATCCTGGCCTTTGTGACTTTCTGTTGCCTCATTGGTCACTTGTGTGCTGATATTGAAAAAACAAATAGTGATACTAGTCCTATTGTTGTTGAAGATCAGGTTCATGTCGGTGTCATTCTCGATATGGGATCAAGGGAAGGAAAGATTATTCTCAGCTGCATATCAATGGCCCTCTCTGATTTTTACCATCTGCATGATAACTACAGTACAAGAGTAGTTCTCCACGGTAAGGATTCCAAAGGTGAACCTCTGCCTGCTCTGTCAGCTGCCCTTGGTCTTTTGGAAAACATCAAAGTGGAATCAATAATTGGTGCACAAACAAGAGCAGAAGCAAACGTTTTGGCAGAATTAGGAGAAGCGGCTAAACTCCCTGTAATGTCTCTTTCTCCTCCTCTGACCAACAATAACTATCCTTTCTTTGTTGAGATCATGCGCGGTGATCAAGCTGCTGAAGTTAGGGGAATCAGTGCACTTATTGAATCAACTCTTTCCCAAGAGATCACACATGGGAGTGTTTCATACAAGAATTCCAATCTTGCTTCCTCCTCATCAAATGAAGAAATCGTTGAAGAGCTCCAAAAGCTGATGAGATTGAAGATTAAGGTATTTGTGGTGCATGTGTCACATATGCTTGCACCACGCCTTTTCTTAATAGCAAATCAGTTAGGAATGATGAGTGAAGGGTATGCTTGGATTGTGACTTCGAGTAGCATGAATTTATTGCAATCCATGAATTTATCTGTTATCGAGTCAATGCAAGGAGTGCTGGGTTTCAAGTCTTTTATTCCAGCATCAATGAGCCTTCACAGTCTTACTTCAAGACTGAGGACGAAGTTTTACCTGGAGGATCCCAATATGGAAGCAATTCGGGAGTTAAGTGCAGATGGGATCTGGGCATATGATACAACTTGGGCTCTAGCAGAAGCAGTTCAAAGAGCAAGGCTTAAAAATTCTACAACTAGATCCTCCAAAGATGGAGCTGTGCTTCTTAGAGAGATATTGCAGACTAGATTAACAGGTTTAAGTGGTGAAATTCAATATGTAAATGGGAAATTGATTCCATCAAAACCATTTGAGATAGTTAATGTTATTGGAAAAGGTGAGATAAAAAGGATTGGATTTTGGCCTTGCAAAGAAGAAGTAAAATCTGGAAAATGGTTAACCCATCAGCAGCTTATTCATAGTAGGAGAAATTTAGCTTCTACAATTGATCTTGAAAGAATCATCTGGCCTGGAGGATCCAAGAGGGACTTGAGCAGTAGTGAAACACTTAAACTAAAAATTGGTGTTCCAGTGAGATATGGGTTCAAGGAACTTGTTCGTGTCGAGCATGATCTTGAAACCAATGCAACTCATGTCACTGGCTTCTCAATAGATGTATTCAAAGCTGCAGTAGGAGCTTTGCCGTATGAAGTGCAGTACGAGTTTATTCCATTTGAGGATGCCAATGGAAATCCTGCAGGGACTAACAATGATCTTGTTTACCAGGTTTATCTCAAGAAATTCGATGCTGTTGTTGGAGATGTTACGATCACAGAGAACAGATCTCGGTATGTTGATTTTACAATCCCATATACTGACTTAGGTGTGGGAATGTTGGTACCCAATGCCAAGGAAAACATGTGGATTTTCTTTAAACCACTTTCAACATATCTTTGGATAACAACTGCTGCTTTCTTTATCCTTACCGGTTTTGTTGTCTGGATATTTGAGCATCCTACTAATGAAAACTTCCAAGGCACAGCAGCGGAACAAATTGGAACTGTATTCTGGTTCTCCTTCTCTACTCTTGTGTTCACTTATACGGAGAAGTTATCAAATAATTTGACAAAGTTTGTAGTGATCATATGGGTGTTTGTAGTGCTTATATTGACGTCGACTTACACTGCAACTTTAGCATCAATGATGACTGTGAAACAGATACAGTTAAACTCAAGAGGAAACAATATAGGTTACCAATCGGGTTCCTTAGGGGTTATAGTGAACTTGAATTTCAAAGGGCTTAAGCCATATCGTTCAAGTGAGGAGTATTTTGTTGCTTTATCAAAAGGAAGCAAGCATAATGGTGTTTCTGGGGTTATTGACGAGGTTCCATACATCAACATCTTCCTTGCAAACTATTCTGCTGAGTACTCGATGATTAAAACCAGGTCTATCACCAATGGTTTTGCCTTTGTTTTCCCTAAAGGTTCGAAATTGGTTCATGATTTGTCAAGGCAAATCGAACGTCTGAGACAGGAGGAAAAGCTTATAGAGATGGAGAAGACCTGGTTTCTTAGAAAAACAACTCTCACGTCTGATGAGGATATTAATAAGAAAGATCCCAATACGATTGACCTCTACGACTTCCGCGGTCTATTCCTTATTAGTGGCGCTTCTTTAGCGTTTGCTCTTTTCTTATTCATAGTTGTATCCCATAGATTTAGAAATCTGATTAGAGGACTAGTGCAGCTGATTGGAAGACAACTACAGCGTTTAAGGTTGTTCCTCTCCAACTTAGTATGCAGTAGAACTGAACAGAACTGCTGCATGTCACTTCCTATGCAATTATACATATATTCTGTCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

866

Amino Acids

97.24

Weight (kDa)

7.95

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 63 - 169 6.4e-10 Receptor family ligand binding region
ANF_receptor PF01094 188 - 375 2.1e-30 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 453 - 546 3.7e-10 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 460 - 769 1.5e-12 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 560 - 802 2.7e-27 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1907
Acc65I GGTACC 1 cut(s) 1630
AccB1I GGYRCC 1 cut(s) 1630
AccI GTMKAC 1 cut(s) 1907
AccII CGCG 2 cut(s) 465, 2382
AciI CCGC 5 cut(s) 389, 465, 1778, 2380, 2382
AclI AACGTT 1 cut(s) 366
AclWI GGATC 8 cut(s) 166, 872, 885, 926, 987, 1263, 1276, 2345
AcsI RAATTY 8 cut(s) 556, 745, 763, 979, 1062, 1222, 1532, 2020
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 1904
AfaI GTAC 4 cut(s) 238, 1451, 1632, 2162
AfiI CCNNNNNNNGG 1 cut(s) 259
AflII CTTAAG 1 cut(s) 2033
AflIII ACRYGT 1 cut(s) 1650
AgeI ACCGGT 1 cut(s) 1718
AhlI ACTAGT 2 cut(s) 104, 2473
AjiI CACGTC 1 cut(s) 2325
AjnI CCWGG 6 cut(s) 33, 870, 1261, 1515, 2177, 2297
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AleI CACNNNNGTG 1 cut(s) 72
Alw21I GWGCWC 3 cut(s) 346, 502, 606
Alw26I GTCTC 2 cut(s) 414, 2257
Alw44I GTGCAC 2 cut(s) 342, 498
AlwI GGATC 8 cut(s) 166, 872, 885, 926, 987, 1263, 1276, 2345
AoxI GGCC 4 cut(s) 36, 198, 1154, 1259
ApaLI GTGCAC 2 cut(s) 342, 498
ApeKI GCWGC 9 cut(s) 186, 296, 476, 1201, 1418, 1700, 1775, 2479, 2556
ApoI RAATTY 8 cut(s) 556, 745, 763, 979, 1062, 1222, 1532, 2020
ArsI GACNNNNNNTTYG 2 cut(s) 2242, 2274
AseI ATTAAT 1 cut(s) 2340
AsiGI ACCGGT 1 cut(s) 1718
Asp700I GAANNNNTTC 3 cut(s) 894, 957, 1347
Asp718I GGTACC 1 cut(s) 1630
AspLEI GCGC 2 cut(s) 465, 2405
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 5 cut(s) 284, 479, 1070, 1142, 2179
AsuII TTCGAA 1 cut(s) 2222
AxyI CCTNAGG 1 cut(s) 1999
BaeGI GKGCMC 2 cut(s) 346, 502
BamHI GGATCC 2 cut(s) 877, 1268
BanI GGYRCC 1 cut(s) 1630
BanII GRGCYC 2 cut(s) 606, 946
BbsI GAAGAC 2 cut(s) 2300, 2497
Bbv12I GWGCWC 3 cut(s) 346, 502, 606
BbvI GCAGC 9 cut(s) 173, 283, 463, 1213, 1405, 1687, 1787, 2491, 2543
BccI CCATC 6 cut(s) 225, 908, 998, 1099, 1203, 2281
BceAI ACGGC 1 cut(s) 1420
BciT130I CCWGG 6 cut(s) 35, 872, 1263, 1517, 2179, 2299
BciVI GTATCC 1 cut(s) 2452
BclI TGATCA 2 cut(s) 469, 1872
BcoDI GTCTC 2 cut(s) 414, 2257
BcuI ACTAGT 2 cut(s) 104, 2473
BfaI CTAG 5 cut(s) 105, 947, 990, 1038, 2474
BfmI CTRYAG 4 cut(s) 232, 1419, 1488, 2498
BfoI RGCGCY 1 cut(s) 2406
BfrI CTTAAG 1 cut(s) 2033
BfuI GTATCC 1 cut(s) 2452
BglII AGATCT 1 cut(s) 1574
BmcAI AGTACT 1 cut(s) 2162
Bme1390I CCNGG 6 cut(s) 35, 872, 1263, 1517, 2179, 2299
BmgBI CACGTC 1 cut(s) 2325
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 5 cut(s) 879, 1270, 1632, 1996, 2121
BmrFI CCNGG 6 cut(s) 35, 872, 1263, 1517, 2179, 2299
BmsI GCATC 5 cut(s) 830, 1462, 1528, 1753, 1937
BpiI GAAGAC 2 cut(s) 2300, 2497
BplI GAGNNNNNCTC 2 cut(s) 234, 266
BpmI CTGGAG 2 cut(s) 893, 1284
Bpu14I TTCGAA 1 cut(s) 2222
BpuEI CTTGAG 3 cut(s) 1303, 1511, 1948
BsaHI GRCGYC 1 cut(s) 1904
BsaJI CCNNGG 5 cut(s) 253, 301, 1641, 1765, 2380
BsaWI WCCGGW 1 cut(s) 1718
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 1 cut(s) 1718
Bse1I ACTGG 2 cut(s) 1322, 1396
Bse21I CCTNAGG 1 cut(s) 1999
BseBI CCWGG 6 cut(s) 35, 872, 1263, 1517, 2179, 2299
BseDI CCNNGG 5 cut(s) 253, 301, 1641, 1765, 2380
BseGI GGATG 3 cut(s) 30, 1477, 1744
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 4 cut(s) 196, 845, 2148, 2252
BseNI ACTGG 2 cut(s) 1322, 1396
BseRI GAGGAG 4 cut(s) 408, 411, 565, 2069
BseSI GKGCMC 2 cut(s) 346, 502
BseXI GCAGC 9 cut(s) 173, 283, 463, 1213, 1405, 1687, 1787, 2491, 2543
BseYI CCCAGC 1 cut(s) 796
BsgI GTGCAG 3 cut(s) 930, 1466, 2498
Bsh1236I CGCG 2 cut(s) 465, 2382
BshFI GGCC 4 cut(s) 38, 200, 1156, 1261
BshNI GGYRCC 1 cut(s) 1630
BshTI ACCGGT 1 cut(s) 1718
BsiHKAI GWGCWC 3 cut(s) 346, 502, 606
BsiSI CCGG 1 cut(s) 1719
BslFI GGGAC 2 cut(s) 1292, 1507
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 2 cut(s) 414, 2257
BsmFI GGGAC 2 cut(s) 1292, 1507
BsnI GGCC 4 cut(s) 38, 200, 1156, 1261
Bsp119I TTCGAA 1 cut(s) 2222
Bsp1286I GDGCHC 4 cut(s) 346, 502, 606, 946
BspACI CCGC 5 cut(s) 389, 465, 1778, 2380, 2382
BspANI GGCC 4 cut(s) 38, 200, 1156, 1261
BspCNI CTCAG 4 cut(s) 195, 846, 2149, 2253
BspFNI CGCG 2 cut(s) 465, 2382
BspHI TCATGA 1 cut(s) 2233
BspLI GGNNCC 5 cut(s) 879, 1270, 1632, 1996, 2121
BspMAI CTGCAG 2 cut(s) 1423, 1492
BspPI GGATC 8 cut(s) 166, 872, 885, 926, 987, 1263, 1276, 2345
BspQI GCTCTTC 1 cut(s) 594
BspT104I TTCGAA 1 cut(s) 2222
BspT107I GGYRCC 1 cut(s) 1630
BspTI CTTAAG 1 cut(s) 2033
BsrFI RCCGGY 1 cut(s) 1718
BsrI ACTGG 2 cut(s) 1322, 1396
BssAI RCCGGY 1 cut(s) 1718
BssECI CCNNGG 5 cut(s) 253, 301, 1641, 1765, 2380
BssNI GRCGYC 1 cut(s) 1904
BssT1I CCWWGG 3 cut(s) 301, 1641, 1765
Bst2UI CCWGG 6 cut(s) 35, 872, 1263, 1517, 2179, 2299
Bst4CI ACNGT 7 cut(s) 28, 236, 257, 839, 1795, 1942, 1956
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 1904
BstAFI CTTAAG 1 cut(s) 2033
BstAPI GCANNNNNTGC 1 cut(s) 793
BstBI TTCGAA 1 cut(s) 2222
BstC8I GCNNGC 3 cut(s) 285, 663, 2087
BstDEI CTNAG 9 cut(s) 182, 854, 1019, 1612, 1999, 2157, 2261, 2306, 2527
BstDSI CCRYGG 2 cut(s) 253, 2380
BstEII GGTNACC 1 cut(s) 1982
BstF5I GGATG 3 cut(s) 30, 1477, 1744
BstFNI CGCG 2 cut(s) 465, 2382
BstH2I RGCGCY 1 cut(s) 2406
BstHHI GCGC 2 cut(s) 465, 2405
BstMAI GTCTC 2 cut(s) 414, 2257
BstMWI GCNNNNNNNGC 6 cut(s) 293, 610, 793, 1427, 1925, 2411
BstNI CCWGG 6 cut(s) 35, 872, 1263, 1517, 2179, 2299
BstNSI RCATGY 3 cut(s) 650, 1654, 2563
BstPI GGTNACC 1 cut(s) 1982
BstSCI CCNGG 6 cut(s) 33, 870, 1261, 1515, 2177, 2297
BstSFI CTRYAG 4 cut(s) 232, 1419, 1488, 2498
BstSLI GKGCMC 2 cut(s) 346, 502
BstUI CGCG 2 cut(s) 465, 2382
BstV1I GCAGC 9 cut(s) 173, 283, 463, 1213, 1405, 1687, 1787, 2491, 2543
BstV2I GAAGAC 2 cut(s) 2300, 2497
BstX2I RGATCY 6 cut(s) 877, 918, 992, 1268, 1574, 2350
BstYI RGATCY 6 cut(s) 877, 918, 992, 1268, 1574, 2350
Bsu36I CCTNAGG 1 cut(s) 1999
BsuI GTATCC 1 cut(s) 2452
BsuRI GGCC 4 cut(s) 38, 200, 1156, 1261
BtgI CCRYGG 2 cut(s) 253, 2380
BtrI CACGTC 1 cut(s) 2325
BtsCI GGATG 3 cut(s) 30, 1477, 1744
BtsI GCAGTG 1 cut(s) 1914
BtsIMutI CAGTG 4 cut(s) 502, 1329, 1389, 1914
Cac8I GCNNGC 3 cut(s) 285, 663, 2087
CciI TCATGA 1 cut(s) 2233
CfoI GCGC 2 cut(s) 465, 2405
Cfr10I RCCGGY 1 cut(s) 1718
Cfr13I GGNCC 1 cut(s) 199
Cfr42I CCGCGG 1 cut(s) 2383
CsiI ACCWGGT 3 cut(s) 1515, 2177, 2297
Csp6I GTAC 4 cut(s) 237, 1450, 1631, 2161
CspAI ACCGGT 1 cut(s) 1718
CviQI GTAC 4 cut(s) 237, 1450, 1631, 2161
DdeI CTNAG 9 cut(s) 182, 854, 1019, 1612, 1999, 2157, 2261, 2306, 2527
DraI TTTAAA 1 cut(s) 1666
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 1 cut(s) 604
Eco130I CCWWGG 3 cut(s) 301, 1641, 1765
Eco24I GRGCYC 2 cut(s) 606, 946
Eco53kI GAGCTC 1 cut(s) 604
Eco57I CTGAAG 1 cut(s) 501
Eco81I CCTNAGG 1 cut(s) 1999
Eco91I GGTNACC 1 cut(s) 1982
EcoICRI GAGCTC 1 cut(s) 604
EcoO65I GGTNACC 1 cut(s) 1982
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 6 cut(s) 33, 870, 1261, 1515, 2177, 2297
EcoT14I CCWWGG 3 cut(s) 301, 1641, 1765
EcoT38I GRGCYC 2 cut(s) 606, 946
ErhI CCWWGG 3 cut(s) 301, 1641, 1765
FalI AAGNNNNNCTT 2 cut(s) 1265, 1297
FaqI GGGAC 2 cut(s) 1292, 1507
FauNDI CATATG 3 cut(s) 657, 928, 1877
FbaI TGATCA 2 cut(s) 469, 1872
FblI GTMKAC 1 cut(s) 1907
FokI GGATG 3 cut(s) 17, 1484, 1731
FriOI GRGCYC 2 cut(s) 606, 946
FspBI CTAG 5 cut(s) 105, 947, 990, 1038, 2474
GlaI GCGC 2 cut(s) 464, 2404
GsaI CCCAGC 1 cut(s) 800
GsuI CTGGAG 2 cut(s) 893, 1284
HaeII RGCGCY 1 cut(s) 2406
HaeIII GGCC 4 cut(s) 38, 200, 1156, 1261
HapII CCGG 1 cut(s) 1719
HhaI GCGC 2 cut(s) 465, 2405
Hin1I GRCGYC 1 cut(s) 1904
Hin6I GCGC 2 cut(s) 463, 2403
HinP1I GCGC 2 cut(s) 463, 2403
HincII GTYRAC 2 cut(s) 1191, 1908
HindII GTYRAC 2 cut(s) 1191, 1908
HindIII AAGCTT 1 cut(s) 2276
HinfI GANTC 7 cut(s) 263, 329, 492, 509, 779, 1087, 1251
HpaI GTTAAC 1 cut(s) 1191
HpaII CCGG 1 cut(s) 1719
HphI GGTGA 5 cut(s) 284, 479, 1070, 1142, 2179
Hpy166II GTNNAC 8 cut(s) 275, 344, 500, 1191, 1513, 1827, 1908, 2014
Hpy188I TCNGA 5 cut(s) 208, 426, 2262, 2329, 2463
Hpy8I GTNNAC 8 cut(s) 275, 344, 500, 1191, 1513, 1827, 1908, 2014
Hpy99I CGWCG 1 cut(s) 1909
HpyAV CCTTC 4 cut(s) 161, 704, 842, 1819
HpyCH4III ACNGT 7 cut(s) 28, 236, 257, 839, 1795, 1942, 1956
HpyCH4IV ACGT 4 cut(s) 366, 1904, 2257, 2324
HpyF10VI GCNNNNNNNGC 6 cut(s) 293, 610, 793, 1427, 1925, 2411
HpyF3I CTNAG 9 cut(s) 182, 854, 1019, 1612, 1999, 2157, 2261, 2306, 2527
HpySE526I ACGT 4 cut(s) 366, 1904, 2257, 2324
Hsp92I GRCGYC 1 cut(s) 1904
HspAI GCGC 2 cut(s) 463, 2403
KpnI GGTACC 1 cut(s) 1634
Ksp22I TGATCA 2 cut(s) 469, 1872
KspAI GTTAAC 1 cut(s) 1191
KspI CCGCGG 1 cut(s) 2383
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 3 cut(s) 609, 1007, 1427
Lsp1109I GCAGC 9 cut(s) 173, 283, 463, 1213, 1405, 1687, 1787, 2491, 2543
LweI GCATC 5 cut(s) 830, 1462, 1528, 1753, 1937
MabI ACCWGGT 3 cut(s) 1515, 2177, 2297
MaeI CTAG 5 cut(s) 105, 947, 990, 1038, 2474
MaeII ACGT 4 cut(s) 366, 1904, 2257, 2324
MaeIII GTNAC 8 cut(s) 43, 65, 651, 727, 1387, 1555, 1982, 2562
MboII GAAGA 8 cut(s) 134, 599, 611, 637, 1178, 2128, 2305, 2502
MfeI CAATTG 1 cut(s) 1236
MflI RGATCY 6 cut(s) 877, 918, 992, 1268, 1574, 2350
MhlI GDGCHC 4 cut(s) 346, 502, 606, 946
MlyI GAGTC 1 cut(s) 788
MmeI TCCRAC 2 cut(s) 1528, 2547
MroXI GAANNNNTTC 3 cut(s) 894, 957, 1347
MslI CAYNNNNRTG 8 cut(s) 72, 138, 194, 222, 323, 540, 1880, 2094
MspA1I CMGCKG 5 cut(s) 186, 296, 1778, 2382, 2482
MspCI CTTAAG 1 cut(s) 2033
MspI CCGG 1 cut(s) 1719
MspR9I CCNGG 6 cut(s) 35, 872, 1263, 1517, 2179, 2299
MunI CAATTG 1 cut(s) 1236
MvaI CCWGG 6 cut(s) 35, 872, 1263, 1517, 2179, 2299
MvnI CGCG 2 cut(s) 465, 2382
MwoI GCNNNNNNNGC 6 cut(s) 293, 610, 793, 1427, 1925, 2411
NdeI CATATG 3 cut(s) 657, 928, 1877
NlaIV GGNNCC 5 cut(s) 879, 1270, 1632, 1996, 2121
NmuCI GTSAC 6 cut(s) 43, 65, 651, 727, 1387, 2562
NspI RCATGY 3 cut(s) 650, 1654, 2563
NspV TTCGAA 1 cut(s) 2222
OliI CACNNNNGTG 1 cut(s) 72
PagI TCATGA 1 cut(s) 2233
PciI ACATGT 1 cut(s) 1650
PciSI GCTCTTC 1 cut(s) 594
PdmI GAANNNNTTC 3 cut(s) 894, 957, 1347
PfeI GAWTC 6 cut(s) 263, 329, 492, 509, 1087, 1251
PinAI ACCGGT 1 cut(s) 1718
PleI GAGTC 1 cut(s) 787
PpsI GAGTC 1 cut(s) 787
PscI ACATGT 1 cut(s) 1650
PshBI ATTAAT 1 cut(s) 2340
Psp124BI GAGCTC 1 cut(s) 606
Psp1406I AACGTT 1 cut(s) 366
Psp6I CCWGG 6 cut(s) 33, 870, 1261, 1515, 2177, 2297
PspEI GGTNACC 1 cut(s) 1982
PspFI CCCAGC 1 cut(s) 796
PspGI CCWGG 6 cut(s) 33, 870, 1261, 1515, 2177, 2297
PspN4I GGNNCC 5 cut(s) 879, 1270, 1632, 1996, 2121
PspPI GGNCC 1 cut(s) 199
PstI CTGCAG 2 cut(s) 1423, 1492
PsuI RGATCY 6 cut(s) 877, 918, 992, 1268, 1574, 2350
PvuII CAGCTG 3 cut(s) 186, 296, 2482
RsaI GTAC 4 cut(s) 238, 1451, 1632, 2162
RsaNI GTAC 4 cut(s) 237, 1450, 1631, 2161
RseI CAYNNNNRTG 8 cut(s) 72, 138, 194, 222, 323, 540, 1880, 2094
SacI GAGCTC 1 cut(s) 606
SacII CCGCGG 1 cut(s) 2383
SalI GTCGAC 1 cut(s) 1906
SapI GCTCTTC 1 cut(s) 594
Sau96I GGNCC 1 cut(s) 199
SbfI CCTGCAGG 1 cut(s) 1492
ScaI AGTACT 1 cut(s) 2162
SchI GAGTC 1 cut(s) 788
ScrFI CCNGG 6 cut(s) 35, 872, 1263, 1517, 2179, 2299
SdaI CCTGCAGG 1 cut(s) 1492
SduI GDGCHC 4 cut(s) 346, 502, 606, 946
SexAI ACCWGGT 3 cut(s) 1515, 2177, 2297
SfaNI GCATC 5 cut(s) 830, 1462, 1528, 1753, 1937
SfcI CTRYAG 4 cut(s) 232, 1419, 1488, 2498
Sfr303I CCGCGG 1 cut(s) 2383
SfuI TTCGAA 1 cut(s) 2222
SgrBI CCGCGG 1 cut(s) 2383
SmiMI CAYNNNNRTG 8 cut(s) 72, 138, 194, 222, 323, 540, 1880, 2094
SmlI CTYRAG 4 cut(s) 1282, 1526, 1963, 2033
SmoI CTYRAG 4 cut(s) 1282, 1526, 1963, 2033
SpeI ACTAGT 2 cut(s) 104, 2473
Sse8387I CCTGCAGG 1 cut(s) 1492
SsiI CCGC 5 cut(s) 389, 465, 1778, 2380, 2382
SspMI CTAG 5 cut(s) 105, 947, 990, 1038, 2474
SstI GAGCTC 1 cut(s) 606
StyD4I CCNGG 6 cut(s) 33, 870, 1261, 1515, 2177, 2297
StyI CCWWGG 3 cut(s) 301, 1641, 1765
TaaI ACNGT 7 cut(s) 28, 236, 257, 839, 1795, 1942, 1956
TaiI ACGT 4 cut(s) 369, 1907, 2260, 2327
TaqI TCGA 9 cut(s) 150, 734, 777, 1356, 1536, 1907, 2165, 2222, 2253
TatI WGTACW 2 cut(s) 236, 2160
TauI GCSGC 1 cut(s) 392
TfiI GAWTC 6 cut(s) 263, 329, 492, 509, 1087, 1251
TscAI CASTG 4 cut(s) 502, 1329, 1396, 1921
TseFI GTSAC 6 cut(s) 43, 65, 651, 727, 1387, 2562
TseI GCWGC 9 cut(s) 186, 296, 476, 1201, 1418, 1700, 1775, 2479, 2556
Tsp45I GTSAC 6 cut(s) 43, 65, 651, 727, 1387, 2562
TspGWI ACGGA 2 cut(s) 1850, 2585
TspRI CASTG 4 cut(s) 502, 1329, 1396, 1921
Vha464I CTTAAG 1 cut(s) 2033
VneI GTGCAC 2 cut(s) 342, 498
VspI ATTAAT 1 cut(s) 2340
XapI RAATTY 8 cut(s) 556, 745, 763, 979, 1062, 1222, 1532, 2020
XceI RCATGY 3 cut(s) 650, 1654, 2563
XmiI GTMKAC 1 cut(s) 1907
XmnI GAANNNNTTC 3 cut(s) 894, 957, 1347
XspI CTAG 5 cut(s) 105, 947, 990, 1038, 2474
ZraI GACGTC 1 cut(s) 1905
ZrmI AGTACT 1 cut(s) 2162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.