MD07G1080800.v1.1

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000294
Physical Location & Seq
Forward (+)
7839105 .. 7842259
3155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1080800.v1.1.491

Sequence Viewer

Length: 2229 bp
ATGTCTCTTTCTGAACCGAGTCATTCTCCACCTAGAAAATATCCCTTCTTCGTCGAGATCACACAGGATGAAACTTTTCAAGCTGCGGGAATAAGTGCCCTCATTGAAGCTTTCAGATGGAGGGATGTTATCCTTTTATATGAGAACTTAAATTATGGGAGGGATATCATTCCCTCTTTGATCCATTCCTTACAAGAGGCAAATGTTAATGTTGCATATAAAAGTTGCATTTCTGCCTCCTCGACAAATGAACAGATCAGCGAGGAGCTCCGCAACATCATGAAGTTGAGGACTACGGTATTTGTGGTGCATATGTCACACTTGCTCGTTCCTCGCTTTCTCCTAACTGTGAAAGAGTTAGGAATGATGAGTGAAGGGTATGCTTGGATTATGACATCAACTAGCCTTAGTTTCTTGCATTCCTCCACGGACTCATCTGTGGTTGAGTCAATGGAAGGGCTGTTGGGTTTTAAGTCTTATATTCCAGCATCAACCCGCCTCCACAAACTTACTTCAAGATTGAGGAGGAAGTTATACACGGAAGATCAAAATATGGAAGTAGGTGAGTTAAGTCCAGATGAAATTTGGGCATATGATGCAACTTGGGCTCTAGCAGAAGCAGTAGAGAGGACAAGGACAAAATATTCTACAAGACTATTGAACCTAATGGATTCGAATAATATTACCAAACAGGGCCTTATTCTTCTTCGGGAGATATTGCATACTAGGTTTACAGGTTTAGGTGGAGAAATTCAATATCCAAATGGGAAGCTAATTTCAAGTGCATTTGAGGTTGTAAATGTGATAGGAAAAGGGGAGAGAAGGGTTGGATTTTGGCCTTGTTACAAGACATTCCACCAAATTAATAACAGAAGAAATTTACTTTCTGCTAACGATCTGAAGACAATCATATGGCCTGGAGGGTCATCAACCTTCGTGAAGGGTTCCAAGCTGCAATTGAGTACTGAAATAAAACTAAGAGTTGGTGTTCCACCAAATAGTGGGTTCAAGGAATTTGTGCGTGTGGACCATGATTTTCAAACCAATAGAACATACTTCTCAGGGTTCTGTATAGACGTATTTGAAGCTGCAGTTAGAGGATTGCCGTATAAAGTACATTATGAGTTTATTTCATTTCAAAATGCCGGTGGATATATGGCCGGGACATACAATGATCTTGTCTACCAGGTTTATCTTCAGAATTACGATGCTGTTGTGGGAGATACGACAATCATATCGAACAGATCTCAGTATGTTGATTTTACAATACCATACACTGACTTAGGCGTGGGAATACTAATAAAAAACGAAAAGAAAGACATGTGGATTTTTTTGAAACCTCTTTCAGGAGACCTTTGGATAACAAGTGCCTGTTTTTTCATCTTAACCGGTTTGGTTGTGTGGGTAATCGAGCGTCCAGTCAACCAAGAATTCCAAGGCACGCCATTGCAGCAAATTGGAACAATATTTTGGTTCTCCTTCTCAACCCTTGTGTTTGCTCATAGGGAGAAGATACTAAGCAACTTGGCAAAGTTTGTAGTGATCATATGGGTGTTTGTTGTGCTTATACTGACTTCCAGTTACACTGCAACATTAGCATCAATGATGACTGTCAAACAAATACAGTTAAACTCGGGGAACTATTTAGGTTACCAATCATTCACCCCTCAAGTTATAGAGAACCCCTCGAGGTTCAAAAGCATTACATTATATGGTTCAATTGAAGAATATGCTGATGCTTTATCAAGAGGAAGTAAGCATGGTGGTGTTTCTGCTATCGTTGACGAGATTCCATACATCAAGGCCTTCCTTGCAAAGTATTCCGCTGGCTACTCAATGATTAAGACCGAGTCTACAACCAATGGTTTCGGCTTTGTCTTCCCAAAAGGTTCGCAATTGGTCCATGACATATCAAGGCAAATTGAAATTCTAAGAGAAGAAGGAAAACTATTAAAAATGGAACAAACTTGGTTTAGTAAAAGACCTCTTACATATGACGACTCGAATACTAATGATCCCAATGCTCTGAACTTCAGCACCTTTCGCGGTTTATTCCTCGTTAGTGGGGTTGCTTCAGCTTTTGCTCTTTCCTTATTCACTATTTTCTCACTCAATGAAAAATGGCATGTCCTGGAGAAATGCAAATGTCGGAACCTGGTTCGAGAAAAGCTGCAGTTTGTAAGGAAGTTCTTGTCTAACAAAGTTTCTAGTCATAATGGAGAAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan Lig_chan-Glu_bd SBP_bac_3

Protein Analysis

743

Amino Acids

84.26

Weight (kDa)

8.76

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 4 - 270 9.2e-54 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 344 - 437 2.1e-12 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 347 - 659 1.1e-10 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 451 - 691 1.9e-30 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1687
AccB7I CCANNNNNTGG 1 cut(s) 1001
AccI GTMKAC 2 cut(s) 1182, 1853
AccII CGCG 1 cut(s) 2046
AciI CCGC 5 cut(s) 86, 271, 496, 1824, 2046
AclWI GGATC 2 cut(s) 175, 2009
AcoI YGGCCR 1 cut(s) 1158
AcsI RAATTY 6 cut(s) 582, 750, 877, 1013, 1430, 1926
AcuI CTGAAG 4 cut(s) 920, 1181, 2017, 2058
AfaI GTAC 2 cut(s) 964, 1116
AfiI CCNNNNNNNGG 2 cut(s) 1001, 1346
AflIII ACRYGT 1 cut(s) 1320
AgeI ACCGGT 1 cut(s) 1388
AjnI CCWGG 4 cut(s) 916, 1185, 2130, 2154
AjuI GAANNNNNNNTTGG 2 cut(s) 1453, 1485
AluBI AGCT 8 cut(s) 83, 110, 268, 772, 952, 1088, 2078, 2170
AluI AGCT 8 cut(s) 83, 110, 268, 772, 952, 1088, 2078, 2170
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 2 cut(s) 9, 1344
AlwI GGATC 2 cut(s) 175, 2009
Ama87I CYCGRG 2 cut(s) 1633, 1687
AoxI GGCC 5 cut(s) 694, 836, 914, 1158, 1803
ApeKI GCWGC 5 cut(s) 83, 952, 1088, 1450, 2170
ApoI RAATTY 6 cut(s) 582, 750, 877, 1013, 1430, 1926
AseI ATTAAT 1 cut(s) 864
AsiGI ACCGGT 1 cut(s) 1388
Asp700I GAANNNNTTC 1 cut(s) 75
AspS9I GGNCC 3 cut(s) 694, 1027, 1900
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 2 cut(s) 575, 1654
AsuII TTCGAA 1 cut(s) 674
AvaI CYCGRG 2 cut(s) 1633, 1687
AvaII GGWCC 2 cut(s) 1027, 1900
BaeGI GKGCMC 1 cut(s) 100
BanII GRGCYC 2 cut(s) 270, 610
BbsI GAAGAC 2 cut(s) 908, 1870
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 5 cut(s) 70, 939, 1075, 1462, 2157
BccI CCATC 1 cut(s) 111
BceAI ACGGC 1 cut(s) 1090
BciT130I CCWGG 4 cut(s) 918, 1187, 2132, 2156
BclI TGATCA 1 cut(s) 1542
BcnI CCSGG 1 cut(s) 1162
BcoDI GTCTC 2 cut(s) 9, 1344
BfaI CTAG 6 cut(s) 33, 402, 611, 726, 2208, 2227
BfmI CTRYAG 2 cut(s) 1089, 2171
BglII AGATCT 1 cut(s) 1244
BisI GCNGC 5 cut(s) 84, 953, 1089, 1451, 2171
BlsI GCNGC 5 cut(s) 85, 954, 1090, 1452, 2172
BmcAI AGTACT 1 cut(s) 964
Bme1390I CCNGG 5 cut(s) 918, 1162, 1187, 2132, 2156
Bme18I GGWCC 2 cut(s) 1027, 1900
BmeT110I CYCGRG 2 cut(s) 1633, 1687
BmgT120I GGNCC 3 cut(s) 694, 1027, 1900
BmiI GGNNCC 2 cut(s) 946, 2153
BmrFI CCNGG 5 cut(s) 918, 1162, 1187, 2132, 2156
BmsI GCATC 5 cut(s) 497, 586, 1198, 1607, 1726
BpiI GAAGAC 2 cut(s) 908, 1870
BplI GAGNNNNNCTC 4 cut(s) 10, 42, 1670, 1702
BpmI CTGGAG 2 cut(s) 939, 2153
Bpu14I TTCGAA 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 1653
BpuMI CCSGG 1 cut(s) 1162
BsaI GGTCTC 1 cut(s) 1344
BsaJI CCNNGG 2 cut(s) 426, 1435
BsaWI WCCGGW 1 cut(s) 1388
Bsc4I CCNNNNNNNGG 2 cut(s) 1001, 1346
Bse118I RCCGGY 2 cut(s) 1145, 1388
Bse1I ACTGG 2 cut(s) 1418, 1578
Bse3DI GCAATG 1 cut(s) 1445
BseBI CCWGG 4 cut(s) 918, 1187, 2132, 2156
BseDI CCNNGG 2 cut(s) 426, 1435
BseGI GGATG 2 cut(s) 73, 130
BseLI CCNNNNNNNGG 2 cut(s) 1001, 1346
BseMI GCAATG 1 cut(s) 1445
BseMII CTCAG 2 cut(s) 1074, 1262
BseNI ACTGG 2 cut(s) 1418, 1578
BseRI GAGGAG 3 cut(s) 229, 278, 538
BseSI GKGCMC 1 cut(s) 100
BseXI GCAGC 5 cut(s) 70, 939, 1075, 1462, 2157
Bsh1236I CGCG 1 cut(s) 2046
BshFI GGCC 5 cut(s) 696, 838, 916, 1160, 1805
BshTI ACCGGT 1 cut(s) 1388
BsiHKAI GWGCWC 1 cut(s) 270
BsiHKCI CYCGRG 2 cut(s) 1633, 1687
BsiSI CCGG 3 cut(s) 1146, 1161, 1389
BslFI GGGAC 1 cut(s) 1177
BslI CCNNNNNNNGG 2 cut(s) 1001, 1346
BsmAI GTCTC 2 cut(s) 9, 1344
BsmFI GGGAC 1 cut(s) 1177
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 5 cut(s) 696, 838, 916, 1160, 1805
Bso31I GGTCTC 1 cut(s) 1344
BsoBI CYCGRG 2 cut(s) 1633, 1687
Bsp119I TTCGAA 1 cut(s) 674
Bsp1286I GDGCHC 3 cut(s) 100, 270, 610
Bsp143I GATC 9 cut(s) 57, 180, 255, 544, 895, 1174, 1244, 1542, 2014
BspACI CCGC 5 cut(s) 86, 271, 496, 1824, 2046
BspANI GGCC 5 cut(s) 696, 838, 916, 1160, 1805
BspCNI CTCAG 2 cut(s) 1073, 1261
BspFNI CGCG 1 cut(s) 2046
BspHI TCATGA 1 cut(s) 279
BspLI GGNNCC 2 cut(s) 946, 2153
BspMAI CTGCAG 2 cut(s) 1093, 2175
BspPI GGATC 2 cut(s) 175, 2009
BspT104I TTCGAA 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 1344
BsrDI GCAATG 1 cut(s) 1445
BsrFI RCCGGY 2 cut(s) 1145, 1388
BsrI ACTGG 2 cut(s) 1418, 1578
BssAI RCCGGY 2 cut(s) 1145, 1388
BssECI CCNNGG 2 cut(s) 426, 1435
BssMI GATC 9 cut(s) 57, 180, 255, 544, 895, 1174, 1244, 1542, 2014
BssT1I CCWWGG 1 cut(s) 1435
Bst2UI CCWGG 4 cut(s) 918, 1187, 2132, 2156
Bst4CI ACNGT 4 cut(s) 298, 349, 1612, 1626
BstAPI GCANNNNNTGC 1 cut(s) 596
BstBI TTCGAA 1 cut(s) 674
BstC8I GCNNGC 2 cut(s) 1442, 1828
BstDEI CTNAG 7 cut(s) 407, 977, 1060, 1248, 1282, 1517, 1931
BstDSI CCRYGG 1 cut(s) 426
BstEII GGTNACC 1 cut(s) 1649
BstENI CCTNNNNNAGG 1 cut(s) 1344
BstF5I GGATG 2 cut(s) 73, 130
BstFNI CGCG 1 cut(s) 2046
BstKTI GATC 9 cut(s) 60, 183, 258, 547, 898, 1177, 1247, 1545, 2017
BstMAI GTCTC 2 cut(s) 9, 1344
BstMBI GATC 9 cut(s) 57, 180, 255, 544, 895, 1174, 1244, 1542, 2014
BstMWI GCNNNNNNNGC 6 cut(s) 596, 605, 1450, 1595, 1811, 2043
BstNI CCWGG 4 cut(s) 918, 1187, 2132, 2156
BstNSI RCATGY 2 cut(s) 1324, 2129
BstPI GGTNACC 1 cut(s) 1649
BstSCI CCNGG 5 cut(s) 916, 1160, 1185, 2130, 2154
BstSFI CTRYAG 2 cut(s) 1089, 2171
BstSLI GKGCMC 1 cut(s) 100
BstUI CGCG 1 cut(s) 2046
BstV1I GCAGC 5 cut(s) 70, 939, 1075, 1462, 2157
BstV2I GAAGAC 2 cut(s) 908, 1870
BstX2I RGATCY 1 cut(s) 1244
BstYI RGATCY 1 cut(s) 1244
BsuRI GGCC 5 cut(s) 696, 838, 916, 1160, 1805
BtgI CCRYGG 1 cut(s) 426
BtsCI GGATG 2 cut(s) 73, 130
BtsI GCAGTG 1 cut(s) 1584
BtsIMutI CAGTG 2 cut(s) 1275, 1584
Cac8I GCNNGC 2 cut(s) 1442, 1828
CciI TCATGA 1 cut(s) 279
Cfr10I RCCGGY 2 cut(s) 1145, 1388
Cfr13I GGNCC 3 cut(s) 694, 1027, 1900
CseI GACGC 1 cut(s) 1403
CsiI ACCWGGT 2 cut(s) 1185, 2154
Csp6I GTAC 2 cut(s) 963, 1115
CspAI ACCGGT 1 cut(s) 1388
CviAII CATG 6 cut(s) 280, 1031, 1321, 1760, 1904, 2126
CviQI GTAC 2 cut(s) 963, 1115
DdeI CTNAG 7 cut(s) 407, 977, 1060, 1248, 1282, 1517, 1931
DpnI GATC 9 cut(s) 59, 182, 257, 546, 897, 1176, 1246, 1544, 2016
DpnII GATC 9 cut(s) 57, 180, 255, 544, 895, 1174, 1244, 1542, 2014
EaeI YGGCCR 1 cut(s) 1158
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 1435
Eco147I AGGCCT 1 cut(s) 1805
Eco24I GRGCYC 2 cut(s) 270, 610
Eco31I GGTCTC 1 cut(s) 1344
Eco32I GATATC 1 cut(s) 166
Eco47I GGWCC 2 cut(s) 1027, 1900
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 4 cut(s) 920, 1181, 2017, 2058
Eco88I CYCGRG 2 cut(s) 1633, 1687
Eco91I GGTNACC 1 cut(s) 1649
EcoICRI GAGCTC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 1344
EcoO109I RGGNCCY 1 cut(s) 694
EcoO65I GGTNACC 1 cut(s) 1649
EcoRI GAATTC 1 cut(s) 1430
EcoRII CCWGG 4 cut(s) 916, 1185, 2130, 2154
EcoRV GATATC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 1435
EcoT38I GRGCYC 2 cut(s) 270, 610
ErhI CCWWGG 1 cut(s) 1435
FaeI CATG 6 cut(s) 283, 1034, 1324, 1763, 1907, 2129
FaqI GGGAC 1 cut(s) 1177
FatI CATG 6 cut(s) 279, 1030, 1320, 1759, 1903, 2125
FauI CCCGC 2 cut(s) 79, 503
FauNDI CATATG 5 cut(s) 312, 592, 911, 1547, 1993
FbaI TGATCA 1 cut(s) 1542
FblI GTMKAC 2 cut(s) 1182, 1853
Fnu4HI GCNGC 5 cut(s) 84, 953, 1089, 1451, 2171
FokI GGATG 2 cut(s) 80, 137
FriOI GRGCYC 2 cut(s) 270, 610
Fsp4HI GCNGC 5 cut(s) 84, 953, 1089, 1451, 2171
FspBI CTAG 6 cut(s) 33, 402, 611, 726, 2208, 2227
GluI GCNGC 5 cut(s) 84, 953, 1089, 1451, 2171
GsuI CTGGAG 2 cut(s) 939, 2153
HaeIII GGCC 5 cut(s) 696, 838, 916, 1160, 1805
HapII CCGG 3 cut(s) 1146, 1161, 1389
HgaI GACGC 1 cut(s) 1403
Hin1II CATG 6 cut(s) 283, 1034, 1324, 1763, 1907, 2129
HincII GTYRAC 2 cut(s) 1423, 1783
HindII GTYRAC 2 cut(s) 1423, 1783
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 7 cut(s) 19, 431, 446, 671, 1789, 1850, 2000
HpaII CCGG 3 cut(s) 1146, 1161, 1389
HphI GGTGA 2 cut(s) 575, 1654
Hpy166II GTNNAC 6 cut(s) 732, 1027, 1183, 1423, 1783, 1854
Hpy188I TCNGA 6 cut(s) 13, 116, 900, 1200, 2028, 2151
Hpy188III TCNNGA 9 cut(s) 55, 280, 516, 575, 710, 937, 1347, 1746, 2162
Hpy8I GTNNAC 6 cut(s) 732, 1027, 1183, 1423, 1783, 1854
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 9 cut(s) 55, 368, 449, 816, 934, 943, 1489, 1816, 1934
HpyCH4III ACNGT 4 cut(s) 298, 349, 1612, 1626
HpyCH4IV ACGT 1 cut(s) 1077
HpyF10VI GCNNNNNNNGC 6 cut(s) 596, 605, 1450, 1595, 1811, 2043
HpyF3I CTNAG 7 cut(s) 407, 977, 1060, 1248, 1282, 1517, 1931
HpySE526I ACGT 1 cut(s) 1077
Hsp92II CATG 6 cut(s) 283, 1034, 1324, 1763, 1907, 2129
Ksp22I TGATCA 1 cut(s) 1542
Kzo9I GATC 9 cut(s) 57, 180, 255, 544, 895, 1174, 1244, 1542, 2014
LmnI GCTCC 2 cut(s) 265, 273
Lsp1109I GCAGC 5 cut(s) 70, 939, 1075, 1462, 2157
LweI GCATC 5 cut(s) 497, 586, 1198, 1607, 1726
MabI ACCWGGT 2 cut(s) 1185, 2154
MaeI CTAG 6 cut(s) 33, 402, 611, 726, 2208, 2227
MaeII ACGT 1 cut(s) 1077
MaeIII GTNAC 4 cut(s) 315, 842, 1580, 1649
MalI GATC 9 cut(s) 59, 182, 257, 546, 897, 1176, 1246, 1544, 2016
MboI GATC 9 cut(s) 57, 180, 255, 544, 895, 1174, 1244, 1542, 2014
MfeI CAATTG 3 cut(s) 956, 1719, 1895
MflI RGATCY 1 cut(s) 1244
MhlI GDGCHC 3 cut(s) 100, 270, 610
MlyI GAGTC 5 cut(s) 28, 425, 455, 1859, 1994
MmeI TCCRAC 2 cut(s) 808, 2129
MroXI GAANNNNTTC 1 cut(s) 75
MseI TTAA 9 cut(s) 149, 207, 471, 569, 864, 1385, 1628, 1842, 1952
MslI CAYNNNNRTG 2 cut(s) 1550, 1764
MspA1I CMGCKG 1 cut(s) 1826
MspI CCGG 3 cut(s) 1146, 1161, 1389
MspR9I CCNGG 5 cut(s) 918, 1162, 1187, 2132, 2156
MunI CAATTG 3 cut(s) 956, 1719, 1895
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 4 cut(s) 918, 1187, 2132, 2156
MvnI CGCG 1 cut(s) 2046
MwoI GCNNNNNNNGC 6 cut(s) 596, 605, 1450, 1595, 1811, 2043
NciI CCSGG 1 cut(s) 1162
NdeI CATATG 5 cut(s) 312, 592, 911, 1547, 1993
NdeII GATC 9 cut(s) 57, 180, 255, 544, 895, 1174, 1244, 1542, 2014
NlaIII CATG 6 cut(s) 283, 1034, 1324, 1763, 1907, 2129
NlaIV GGNNCC 2 cut(s) 946, 2153
NmuCI GTSAC 1 cut(s) 315
NspI RCATGY 2 cut(s) 1324, 2129
NspV TTCGAA 1 cut(s) 674
PaeR7I CTCGAG 1 cut(s) 1687
PagI TCATGA 1 cut(s) 279
PceI AGGCCT 1 cut(s) 1805
PciI ACATGT 1 cut(s) 1320
PctI GAATGC 1 cut(s) 418
PdmI GAANNNNTTC 1 cut(s) 75
PfeI GAWTC 2 cut(s) 671, 1789
PflFI GACNNNGTC 1 cut(s) 1849
PflMI CCANNNNNTGG 1 cut(s) 1001
PfoI TCCNGGA 1 cut(s) 2130
PinAI ACCGGT 1 cut(s) 1388
PkrI GCNGC 5 cut(s) 85, 954, 1090, 1452, 2172
PleI GAGTC 5 cut(s) 27, 425, 454, 1858, 1994
PpsI GAGTC 5 cut(s) 27, 425, 454, 1858, 1994
PscI ACATGT 1 cut(s) 1320
PshBI ATTAAT 1 cut(s) 864
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 4 cut(s) 916, 1185, 2130, 2154
PspEI GGTNACC 1 cut(s) 1649
PspGI CCWGG 4 cut(s) 916, 1185, 2130, 2154
PspN4I GGNNCC 2 cut(s) 946, 2153
PspPI GGNCC 3 cut(s) 694, 1027, 1900
PspXI VCTCGAGB 1 cut(s) 1687
PstI CTGCAG 2 cut(s) 1093, 2175
PsuI RGATCY 1 cut(s) 1244
PsyI GACNNNGTC 1 cut(s) 1849
RsaI GTAC 2 cut(s) 964, 1116
RsaNI GTAC 2 cut(s) 963, 1115
RseI CAYNNNNRTG 2 cut(s) 1550, 1764
SacI GAGCTC 1 cut(s) 270
SaqAI TTAA 9 cut(s) 149, 207, 471, 569, 864, 1385, 1628, 1842, 1952
SatI GCNGC 5 cut(s) 84, 953, 1089, 1451, 2171
Sau3AI GATC 9 cut(s) 57, 180, 255, 544, 895, 1174, 1244, 1542, 2014
Sau96I GGNCC 3 cut(s) 694, 1027, 1900
ScaI AGTACT 1 cut(s) 964
SchI GAGTC 5 cut(s) 28, 425, 455, 1859, 1994
ScrFI CCNGG 5 cut(s) 918, 1162, 1187, 2132, 2156
SduI GDGCHC 3 cut(s) 100, 270, 610
SexAI ACCWGGT 2 cut(s) 1185, 2154
SfaNI GCATC 5 cut(s) 497, 586, 1198, 1607, 1726
SfcI CTRYAG 2 cut(s) 1089, 2171
Sfr274I CTCGAG 1 cut(s) 1687
SfuI TTCGAA 1 cut(s) 674
SinI GGWCC 2 cut(s) 1027, 1900
SlaI CTCGAG 1 cut(s) 1687
SmiMI CAYNNNNRTG 2 cut(s) 1550, 1764
SmlI CTYRAG 2 cut(s) 1668, 1687
SmoI CTYRAG 2 cut(s) 1668, 1687
SseBI AGGCCT 1 cut(s) 1805
SsiI CCGC 5 cut(s) 86, 271, 496, 1824, 2046
SspI AATATT 3 cut(s) 644, 682, 1467
SspMI CTAG 6 cut(s) 33, 402, 611, 726, 2208, 2227
SstI GAGCTC 1 cut(s) 270
StuI AGGCCT 1 cut(s) 1805
StyD4I CCNGG 5 cut(s) 916, 1160, 1185, 2130, 2154
StyI CCWWGG 1 cut(s) 1435
TaaI ACNGT 4 cut(s) 298, 349, 1612, 1626
TaiI ACGT 1 cut(s) 1080
TaqI TCGA 8 cut(s) 54, 242, 674, 1238, 1410, 1688, 2003, 2161
TaqII GACCGA 1 cut(s) 1862
TatI WGTACW 2 cut(s) 962, 1114
TfiI GAWTC 2 cut(s) 671, 1789
Tru1I TTAA 9 cut(s) 149, 207, 471, 569, 864, 1385, 1628, 1842, 1952
Tru9I TTAA 9 cut(s) 149, 207, 471, 569, 864, 1385, 1628, 1842, 1952
TscAI CASTG 2 cut(s) 1282, 1591
TseFI GTSAC 1 cut(s) 315
TseI GCWGC 5 cut(s) 83, 952, 1088, 1450, 2170
Tsp45I GTSAC 1 cut(s) 315
TspDTI ATGAA 7 cut(s) 84, 264, 296, 594, 1122, 1369, 2130
TspGWI ACGGA 2 cut(s) 443, 554
TspRI CASTG 2 cut(s) 1282, 1591
Tth111I GACNNNGTC 1 cut(s) 1849
Van91I CCANNNNNTGG 1 cut(s) 1001
VpaK11BI GGWCC 2 cut(s) 1027, 1900
VspI ATTAAT 1 cut(s) 864
XagI CCTNNNNNAGG 1 cut(s) 1344
XapI RAATTY 6 cut(s) 582, 750, 877, 1013, 1430, 1926
XceI RCATGY 2 cut(s) 1324, 2129
XcmI CCANNNNNNNNNTGG 1 cut(s) 582
XhoI CTCGAG 1 cut(s) 1687
XmiI GTMKAC 2 cut(s) 1182, 1853
XmnI GAANNNNTTC 1 cut(s) 75
XspI CTAG 6 cut(s) 33, 402, 611, 726, 2208, 2227
ZrmI AGTACT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.