RchiOBHm_Chr5g0063031

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000294
Physical Location & Seq
Forward (+)
68795431 .. 68797419
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33919

Sequence Viewer

Length: 1689 bp
ATGGAGTTTCGAGGGTTTAAGAAAACAATCATCCTTTCCTCCTTTGTGACCCTATGTTGCCTCATTGGTCCCTTGTGTGCTGATACTGAAAATATAAATAATGTTGTTAATACCATGAATGTGGAAGATCAAGTTCATATTGGTGTAATTCTAGACATGGGATCACTGGAAGGAAAGATTGTTCTCAGCTGTATTTCAATGGCCCTATCTGATTTTTACCATCTGCATAATAACTACAGCACAAGAGTAATTCTCCACAATAAGGATTCCAAAGGCAAACCTCTACCTGCTATGTCAGCTGCTCTTAGTCTGCTGGATAACATCAAAGTGCAAGCAATAATTGGTGCACGAACTAAAACAGAGGCAAACCTTTTGGCAGAACTAGGAGAAGCGGTTCAACTCCCCGTCATGTCTTTTTCTCCTTCCTTCACTGACAATAAATATGCCTTCTTTGTTGAGATCATGCGCGCTGATCAAGCTGCTGAAGTTAAGGGAATCAGTGCCCTTGTTGATGTTTTCAAATGGAGGGATATTATCCTTCTATATGATAACAAAGAATATGAGAGAGACTTCATTCCATCATTAGTCAACTCCTTCCAAGAGATCACACATGGGAGTATCGACTGCAAAAGTTCAAATATTGCTTCCTCATCATCAAATGAAGAAATCATGGCAGAGCTCCAAAAGCTGATGAAACTGAAGATTAAGGTATTTGTGGTGCAGGTGTCACATTTGCTCGCACCTCGCCTTTTCTTATGTGCAAATAAGTTGGGGATGATGATTGAAGGGTATACTTGGATTGTGACATCAACTAGCATGAATTTCTTAAATTCCATGGATTTGTCTGTTATTGAGTCAATGCAAGGAGTGCTGGGTTTCAGATCTTCTATTCCAGCATCATTGAGCCTTCATAGTCTTACTTCAAGATTGAGGAGAAAGTTTCAACTGGAGGATCCCAATATGGAAGCAATTCGAGAGTTAAGCGCAGAAGGAATCTGGGCGTTTGATGCAACTTGGGCTCTAGCAGAAGCAGTTGAAAGAGCAAGGCCTAAAAATTCTACCACTAGATCCTCCAAAGGAGTTGTGGTTCTCAGAGAGATAATGCAAAGTAGATTTAATGGTTTAAGTGGTGAAATTCATTATCTAAATGGGAAATTGATTTCATCAGAACCATTTGAGATAGTTAATGTTATAGGAGAAGGGGAGATCAAAAGGATTGGATTTTGGCCTTGCAAAGAGGAACAAAAAACCAGAAAAGGGTCACCCAGCAGCACCTTAATTACTAATGATTGGAAAAATTTAGTTTCAACTATTGATATCGGAAAAATCATATGGCCTGGAGGATCCAAGAGGGAATCATCAATACTTAAAATTAGAAAACTAAGAATTGGCGTTCCAGTGAGAATCGGGTTCAAGGAACTTGTTCGTATCGAGCATGATCTTGAAATCAATAAAACTCATGTCACTGGCTTCTCTATAGATGTATTCAAAGCTGCAATAGGAGCTTTGTCTTATGAAGAGGATGTGGATTACGAGTTTATTCCATTTGAGGATGCCAATGGAAACCCTGCTGGTAGTTACAATGATCTTGTGCACCAGGTTTATCTTGAGGTATATGTATGCTTAATATGTTTACCAACTCGTTATTACTGCTTCATTCATATGCCATGCATTCTAATTACAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

562

Amino Acids

62.91

Weight (kDa)

6.23

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 45 - 378 2.3e-10 Periplasmic binding protein
ANF_receptor PF01094 63 - 398 5.5e-52 Receptor family ligand binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 712
Acc36I ACCTGC 2 cut(s) 295, 712
AccI GTMKAC 1 cut(s) 791
AccII CGCG 1 cut(s) 468
AciI CCGC 1 cut(s) 392
AclWI GGATC 6 cut(s) 169, 947, 960, 1062, 1338, 1351
AcsI RAATTY 5 cut(s) 820, 829, 1054, 1134, 1297
AcuI CTGAAG 2 cut(s) 504, 719
AfiI CCNNNNNNNGG 2 cut(s) 262, 1257
AjnI CCWGG 2 cut(s) 1336, 1596
AloI GAACNNNNNNTCC 6 cut(s) 165, 197, 378, 410, 1071, 1103
AluBI AGCT 8 cut(s) 189, 299, 479, 679, 688, 1493, 1505, 1685
AluI AGCT 8 cut(s) 189, 299, 479, 679, 688, 1493, 1505, 1685
Alw21I GWGCWC 3 cut(s) 349, 681, 1596
Alw26I GTCTC 1 cut(s) 561
Alw44I GTGCAC 2 cut(s) 345, 1592
AlwI GGATC 6 cut(s) 169, 947, 960, 1062, 1338, 1351
AoxI GGCC 4 cut(s) 201, 1046, 1226, 1334
ApaLI GTGCAC 2 cut(s) 345, 1592
ApeKI GCWGC 4 cut(s) 299, 479, 1269, 1493
ApoI RAATTY 5 cut(s) 820, 829, 1054, 1134, 1297
Asp700I GAANNNNTTC 3 cut(s) 393, 969, 1422
AspLEI GCGC 3 cut(s) 468, 470, 986
AspS9I GGNCC 2 cut(s) 68, 202
AsuHPI GGTGA 2 cut(s) 1142, 1254
AvaII GGWCC 1 cut(s) 68
BaeGI GKGCMC 3 cut(s) 349, 505, 1596
BamHI GGATCC 2 cut(s) 952, 1343
BanII GRGCYC 2 cut(s) 681, 1021
Bbv12I GWGCWC 3 cut(s) 349, 681, 1596
BbvI GCAGC 4 cut(s) 286, 466, 1281, 1480
BccI CCATC 2 cut(s) 228, 586
BciT130I CCWGG 2 cut(s) 1338, 1598
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 561
BfaI CTAG 5 cut(s) 152, 383, 813, 1022, 1065
BfmI CTRYAG 2 cut(s) 235, 1476
BfuAI ACCTGC 2 cut(s) 295, 712
BglII AGATCT 1 cut(s) 881
BisI GCNGC 4 cut(s) 300, 480, 1270, 1494
BlsI GCNGC 4 cut(s) 301, 481, 1271, 1495
Bme1390I CCNGG 2 cut(s) 1338, 1598
Bme18I GGWCC 1 cut(s) 68
BmgT120I GGNCC 2 cut(s) 68, 202
BmiI GGNNCC 3 cut(s) 70, 954, 1345
BmrFI CCNGG 2 cut(s) 1338, 1598
BmsI GCATC 3 cut(s) 905, 997, 1543
BplI GAGNNNNNCTC 2 cut(s) 237, 269
BpmI CTGGAG 2 cut(s) 968, 1359
BpuEI CTTGAG 1 cut(s) 1628
BsaJI CCNNGG 1 cut(s) 834
BsaXI ACNNNNNCTCC 4 cut(s) 378, 408, 1071, 1101
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 1257
Bse1I ACTGG 4 cut(s) 171, 951, 1397, 1471
BseBI CCWGG 2 cut(s) 1338, 1598
BseDI CCNNGG 1 cut(s) 834
BseGI GGATG 4 cut(s) 30, 780, 1528, 1558
BseLI CCNNNNNNNGG 2 cut(s) 262, 1257
BseMII CTCAG 2 cut(s) 199, 1105
BseNI ACTGG 4 cut(s) 171, 951, 1397, 1471
BsePI GCGCGC 1 cut(s) 466
BseRI GAGGAG 1 cut(s) 946
BseSI GKGCMC 3 cut(s) 349, 505, 1596
BseXI GCAGC 4 cut(s) 286, 466, 1281, 1480
BseYI CCCAGC 2 cut(s) 871, 1265
BsgI GTGCAG 1 cut(s) 740
Bsh1236I CGCG 1 cut(s) 468
BshFI GGCC 4 cut(s) 203, 1048, 1228, 1336
BsiHKAI GWGCWC 3 cut(s) 349, 681, 1596
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 262, 1257
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 1 cut(s) 54
BsmI GAATGC 1 cut(s) 1671
BsnI GGCC 4 cut(s) 203, 1048, 1228, 1336
Bsp1286I GDGCHC 5 cut(s) 349, 505, 681, 1021, 1596
Bsp19I CCATGG 1 cut(s) 834
BspACI CCGC 1 cut(s) 392
BspANI GGCC 4 cut(s) 203, 1048, 1228, 1336
BspCNI CTCAG 2 cut(s) 198, 1104
BspFNI CGCG 1 cut(s) 468
BspLI GGNNCC 3 cut(s) 70, 954, 1345
BspMI ACCTGC 2 cut(s) 295, 712
BspPI GGATC 6 cut(s) 169, 947, 960, 1062, 1338, 1351
BsrI ACTGG 4 cut(s) 171, 951, 1397, 1471
BssECI CCNNGG 1 cut(s) 834
BssHII GCGCGC 1 cut(s) 466
BssNAI GTATAC 1 cut(s) 792
BssT1I CCWWGG 1 cut(s) 834
Bst1107I GTATAC 1 cut(s) 792
Bst2UI CCWGG 2 cut(s) 1338, 1598
Bst6I CTCTTC 1 cut(s) 1512
BstAPI GCANNNNNTGC 1 cut(s) 868
BstC8I GCNNGC 3 cut(s) 333, 468, 738
BstDEI CTNAG 4 cut(s) 185, 305, 1091, 1382
BstDSI CCRYGG 1 cut(s) 834
BstEII GGTNACC 1 cut(s) 1260
BstF5I GGATG 4 cut(s) 30, 780, 1528, 1558
BstFNI CGCG 1 cut(s) 468
BstHHI GCGC 3 cut(s) 468, 470, 986
BstMAI GTCTC 1 cut(s) 561
BstMWI GCNNNNNNNGC 7 cut(s) 296, 476, 685, 868, 1007, 1016, 1502
BstNI CCWGG 2 cut(s) 1338, 1598
BstPI GGTNACC 1 cut(s) 1260
BstSCI CCNGG 2 cut(s) 1336, 1596
BstSFI CTRYAG 2 cut(s) 235, 1476
BstSLI GKGCMC 3 cut(s) 349, 505, 1596
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 4 cut(s) 286, 466, 1281, 1480
BstX2I RGATCY 4 cut(s) 881, 952, 1067, 1343
BstXI CCANNNNNNTGG 1 cut(s) 121
BstYI RGATCY 4 cut(s) 881, 952, 1067, 1343
BstZ17I GTATAC 1 cut(s) 792
BsuRI GGCC 4 cut(s) 203, 1048, 1228, 1336
BtgI CCRYGG 1 cut(s) 834
BtsCI GGATG 4 cut(s) 30, 780, 1528, 1558
BtsIMutI CAGTG 5 cut(s) 164, 429, 505, 1404, 1464
BveI ACCTGC 2 cut(s) 295, 712
Cac8I GCNNGC 3 cut(s) 333, 468, 738
CfoI GCGC 3 cut(s) 468, 470, 986
Cfr13I GGNCC 2 cut(s) 68, 202
CsiI ACCWGGT 1 cut(s) 1596
DdeI CTNAG 4 cut(s) 185, 305, 1091, 1382
Eam1104I CTCTTC 1 cut(s) 1512
EarI CTCTTC 1 cut(s) 1512
Ecl136II GAGCTC 1 cut(s) 679
Eco130I CCWWGG 1 cut(s) 834
Eco147I AGGCCT 1 cut(s) 1048
Eco24I GRGCYC 2 cut(s) 681, 1021
Eco32I GATATC 1 cut(s) 1318
Eco47I GGWCC 1 cut(s) 68
Eco53kI GAGCTC 1 cut(s) 679
Eco57I CTGAAG 2 cut(s) 504, 719
Eco91I GGTNACC 1 cut(s) 1260
EcoICRI GAGCTC 1 cut(s) 679
EcoO65I GGTNACC 1 cut(s) 1260
EcoRII CCWGG 2 cut(s) 1336, 1596
EcoRV GATATC 1 cut(s) 1318
EcoT14I CCWWGG 1 cut(s) 834
EcoT22I ATGCAT 1 cut(s) 1673
EcoT38I GRGCYC 2 cut(s) 681, 1021
ErhI CCWWGG 1 cut(s) 834
FaqI GGGAC 1 cut(s) 54
FauNDI CATATG 2 cut(s) 1331, 1662
FbaI TGATCA 1 cut(s) 472
FblI GTMKAC 1 cut(s) 791
Fnu4HI GCNGC 4 cut(s) 300, 480, 1270, 1494
FokI GGATG 4 cut(s) 17, 787, 1535, 1565
FriOI GRGCYC 2 cut(s) 681, 1021
Fsp4HI GCNGC 4 cut(s) 300, 480, 1270, 1494
FspBI CTAG 5 cut(s) 152, 383, 813, 1022, 1065
GlaI GCGC 3 cut(s) 467, 469, 985
GluI GCNGC 4 cut(s) 300, 480, 1270, 1494
GsaI CCCAGC 2 cut(s) 875, 1269
GsuI CTGGAG 2 cut(s) 968, 1359
HaeIII GGCC 4 cut(s) 203, 1048, 1228, 1336
HhaI GCGC 3 cut(s) 468, 470, 986
Hin6I GCGC 3 cut(s) 466, 468, 984
HinP1I GCGC 3 cut(s) 466, 468, 984
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HinfI GANTC 6 cut(s) 266, 495, 854, 993, 1355, 1404
HphI GGTGA 2 cut(s) 1142, 1254
Hpy166II GTNNAC 5 cut(s) 347, 589, 792, 1594, 1634
Hpy188I TCNGA 5 cut(s) 211, 881, 1094, 1168, 1322
Hpy188III TCNNGA 5 cut(s) 152, 924, 974, 1442, 1607
Hpy8I GTNNAC 5 cut(s) 347, 589, 792, 1594, 1634
HpyF10VI GCNNNNNNNGC 7 cut(s) 296, 476, 685, 868, 1007, 1016, 1502
HpyF3I CTNAG 4 cut(s) 185, 305, 1091, 1382
HspAI GCGC 3 cut(s) 466, 468, 984
Ksp22I TGATCA 1 cut(s) 472
LmnI GCTCC 2 cut(s) 684, 1502
Lsp1109I GCAGC 4 cut(s) 286, 466, 1281, 1480
LweI GCATC 3 cut(s) 905, 997, 1543
MabI ACCWGGT 1 cut(s) 1596
MaeI CTAG 5 cut(s) 152, 383, 813, 1022, 1065
MaeIII GTNAC 6 cut(s) 46, 726, 802, 1260, 1462, 1577
MboII GAAGA 5 cut(s) 137, 674, 712, 876, 1529
MflI RGATCY 4 cut(s) 881, 952, 1067, 1343
MhlI GDGCHC 5 cut(s) 349, 505, 681, 1021, 1596
MlyI GAGTC 1 cut(s) 863
Mph1103I ATGCAT 1 cut(s) 1673
MroXI GAANNNNTTC 3 cut(s) 393, 969, 1422
MslI CAYNNNNRTG 4 cut(s) 119, 141, 326, 1661
MspA1I CMGCKG 2 cut(s) 189, 299
MspR9I CCNGG 2 cut(s) 1338, 1598
Mva1269I GAATGC 1 cut(s) 1671
MvaI CCWGG 2 cut(s) 1338, 1598
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 7 cut(s) 296, 476, 685, 868, 1007, 1016, 1502
NcoI CCATGG 1 cut(s) 834
NdeI CATATG 2 cut(s) 1331, 1662
NlaIV GGNNCC 3 cut(s) 70, 954, 1345
NmuCI GTSAC 5 cut(s) 46, 726, 802, 1260, 1462
NsiI ATGCAT 1 cut(s) 1673
PaqCI CACCTGC 1 cut(s) 712
PauI GCGCGC 1 cut(s) 466
PceI AGGCCT 1 cut(s) 1048
PctI GAATGC 1 cut(s) 1671
PdmI GAANNNNTTC 3 cut(s) 393, 969, 1422
PfeI GAWTC 5 cut(s) 266, 495, 993, 1355, 1404
PkrI GCNGC 4 cut(s) 301, 481, 1271, 1495
PleI GAGTC 1 cut(s) 862
PpsI GAGTC 1 cut(s) 862
Psp124BI GAGCTC 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 1336, 1596
PspEI GGTNACC 1 cut(s) 1260
PspFI CCCAGC 2 cut(s) 871, 1265
PspGI CCWGG 2 cut(s) 1336, 1596
PspN4I GGNNCC 3 cut(s) 70, 954, 1345
PspPI GGNCC 2 cut(s) 68, 202
PsuI RGATCY 4 cut(s) 881, 952, 1067, 1343
PteI GCGCGC 1 cut(s) 466
PvuII CAGCTG 2 cut(s) 189, 299
RseI CAYNNNNRTG 4 cut(s) 119, 141, 326, 1661
SacI GAGCTC 1 cut(s) 681
SatI GCNGC 4 cut(s) 300, 480, 1270, 1494
Sau96I GGNCC 2 cut(s) 68, 202
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 2 cut(s) 1338, 1598
SduI GDGCHC 5 cut(s) 349, 505, 681, 1021, 1596
SexAI ACCWGGT 1 cut(s) 1596
SfaNI GCATC 3 cut(s) 905, 997, 1543
SfcI CTRYAG 2 cut(s) 235, 1476
SinI GGWCC 1 cut(s) 68
SmiMI CAYNNNNRTG 4 cut(s) 119, 141, 326, 1661
SmlI CTYRAG 1 cut(s) 1607
SmoI CTYRAG 1 cut(s) 1607
SseBI AGGCCT 1 cut(s) 1048
SsiI CCGC 1 cut(s) 392
SspI AATATT 1 cut(s) 640
SspMI CTAG 5 cut(s) 152, 383, 813, 1022, 1065
SstI GAGCTC 1 cut(s) 681
StuI AGGCCT 1 cut(s) 1048
StyD4I CCNGG 2 cut(s) 1336, 1596
StyI CCWWGG 1 cut(s) 834
TaqI TCGA 4 cut(s) 10, 621, 973, 1431
TfiI GAWTC 5 cut(s) 266, 495, 993, 1355, 1404
TscAI CASTG 5 cut(s) 171, 436, 505, 1404, 1471
TseFI GTSAC 5 cut(s) 46, 726, 802, 1260, 1462
TseI GCWGC 4 cut(s) 299, 479, 1269, 1493
Tsp45I GTSAC 5 cut(s) 46, 726, 802, 1260, 1462
TspRI CASTG 5 cut(s) 171, 436, 505, 1404, 1471
VneI GTGCAC 2 cut(s) 345, 1592
VpaK11BI GGWCC 1 cut(s) 68
XapI RAATTY 5 cut(s) 820, 829, 1054, 1134, 1297
XbaI TCTAGA 1 cut(s) 151
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XmiI GTMKAC 1 cut(s) 791
XmnI GAANNNNTTC 3 cut(s) 393, 969, 1422
XspI CTAG 5 cut(s) 152, 383, 813, 1022, 1065
Zsp2I ATGCAT 1 cut(s) 1673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.