Rw5G038950

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000294
Physical Location & Seq
Forward (+)
68862803 .. 68873785
10983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G038950.1

Sequence Viewer

Length: 2628 bp
ATGGAGTTTCGAGGGTCTAAGAAAACAATCATCCTTTCCTCCTTAGTGACCCTATGTTGCCTCATTGGTCCCTTGTGTGCTGATACTGAAAATATAAATAATGTTGTTAATACCATGAATGTGGAAGATCAAGTTCATATTGGTGTGATTCTAGACATGGGATCACTGGAAGGAAAGATTGTTCTCAGCTGTATTTCAATGGCCCTATCTGATTTTTACCATCTGCATAATAACTACAGCACAAGAGTAATTCTCCACAATAAGGATTCCAAAGGCAAACCTCTACCTGCTATGTCAGCTGCTCTTAGTCTGTTGGATAACATCAAAGTGGAAGCAATAATTGGTGCACGAACTAAAACAGAGGCAAACCTTTTGGCAGAACTAGGAGAAGCGGTTCAACTCCCCGTCATGTCTTTTTCTCCTTCCTTCACTGACAATAAATATGCCTTCTTTGTTGAGATCATGAGCGCTAATCAAGCTGCTGAAGTTAAGGGAATCAGTGCCCTTGTTGATGTTTTCAAATGGAGGGATATTATCCTTCTATATGATAACAAAGAATATGAGAGAGACTTCATTCCATCATTAGTCAACTCCTTCCAAGAGATCACACATGGGAGTATCGACTGCAAAAGTTCAAATATTGCTTCCTCATCATCAAATGAAGAAATCATGGAAGAGCTCCAAAAGCTGATGAAACTGAAGATTAAGGTATTTGTGGTGCAGGTGTCACATTTGCTTGCACCTCGCCTTTTCTTATGTGCAAATAAGTTGGGGATGATGATTGAAGGGTATACTTGGATTGTGACATCAACTAGCATGAATTTCTTAAATTCCATGGATTTGTCTGTTATTGAGTCAATGCAAGGAGTGCTGGGGTTCAGATCTTCTATTCCAGCATCATTGAGCCTTCATAGTCTTACTTCAAGATTGAGGAGAAAGTTTCAACTGGAGGATCCCACTATGGAAGCAATTCGAGAGTTAAGCGCAGAAGGAATCTGGGCGTATGATGCAACTTGGGCTCTAGCAGAAGCAGTTGAAAGAGCAAGGCCTAAAAATTCTACCACTAGATCCTCCAAAGGAGTTGTGGTTCTCAGAGAGATAATGCAAAGTAGATTTAATGGTTTAAGTGGTGAAATTCATTATCTAAATGGGAAATTGATTTCATCAGAACCATTTGAGATAGTTAATGTTATAGGAGAAGGGGAGATCAAAAGCATTGGATTTTGGCCTTGCAAAGAGGAACAAAATATAATGTTAAGTCCACATATTCTAACACAAAAAACCAGAAAAGGGTCACCCAGCAGCACCTTAATTACTAATGATTGGAAAAATTTAGTTTCAACTATTGATATCGGAAAAATCATATGGCCTGGAGGATCCAAGAGGGAATCATCAATACATAAAATTAGAAAACTAAGAATTGGTGTTCCAGTGAGAATCGGGTTCAAGGAACTTGTTCGTATCGAGCATGATCTTGAAATCAATAAAACTCATGTCACTGGCTTCTCTATAGATGTATTCAAAGCTGCAATAGGAGCTTTGTCTTATGAAGAGGATGTGGATTACGAGTTTATTCCATTTGAGGATGCCAATGGAAACCCTGCTGGTAGTTACAATGATCTTGTGCACCAGGTTTATCTTGAGAAATACGATGCTGCTGTTGGAGATATCACGATCACATCAAACAGATCTCAATATGTTGATTTTACACTGCCATATACCGATTTTGGTTTGGGATTGTTGGCACGAAATGAAAAGAACATGTGGATTTTCTTCAAACCACTTTCCCCATATCTCTGGATTACAAGTGCTGCTTTCTTTGTCCTAACCGGCTGTGTTGTTTGGATAATTGAGGGTCCTACTAACGAAGAATTCCAAGGCACAGCAGAGGAGCAAATTGGAACAGTAATGTGGTTCTCCTTCTCAACTCTTGTGTTTGCTCTTGTTTGTAGTGATCATATGGGTGTTTATGTGACATCCAGTTACACTGCAACTTTAGCATCAATGATGACAGTAAAACAGATTCACTTAAATTCAATAGGGAACAATATAGGTTACCAATCAGGTTCACTAGGAGCTATAGTGAACTTCAATATTGCAGGGAATCATTCATATATTTCAGCTGAAGACTATTTTGATGCCTTATCCAAAGGAAGCAAGCATGGTGGTGTTGCTGGGATTATTGACGAAGTTCCGTACATCAACATCTTCCTTGGAAAGTATACTGATGATGATTTCTCAATGATTAAAACCAGTTCTTACACCAATGGTTTTGCCTTTGCTTTCCCTCAAGGTTCAAAATTGGCTCGTAACGTGTCGAAGCAAATTGAATATCTGAGAGAGGAAGGAAAGCTTATGGAGATGGAAAAGACCTGGTTTCATAGAAGAACAGAAGACGAAGATAAATCTAAGAGCGATGCCGATACGATTGATCTCTACGACTTTCGTGGATTATTCCTTATTAGTGGAGGTTCTTTGCTTATTGCTCTAGTTTTATCTCAAATATTTAGAAATCTGATTCGAGGACTACTACAGCTGATTGGAAGAGAACTACAGCGTTTAAGGATGTTCGTCTACGAGGTTAATTTCTTTATGCTAAAGTTGGATTTTTGGGTAGCGATTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

875

Amino Acids

98.0

Weight (kDa)

5.64

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 45 - 378 2.4e-10 Periplasmic binding protein
ANF_receptor PF01094 63 - 398 5.6e-52 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 492 - 585 2.5e-10 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 497 - 794 2.2e-11 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 599 - 824 7.2e-11 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2626
AarI CACCTGC 1 cut(s) 712
Acc36I ACCTGC 2 cut(s) 295, 712
AccI GTMKAC 3 cut(s) 791, 2222, 2574
AciI CCGC 1 cut(s) 392
AclWI GGATC 6 cut(s) 169, 947, 960, 1062, 1371, 1384
AcsI RAATTY 7 cut(s) 820, 829, 1054, 1134, 1330, 1871, 2032
AcuI CTGAAG 3 cut(s) 504, 719, 2145
AfaI GTAC 1 cut(s) 2198
AfeI AGCGCT 1 cut(s) 469
AfiI CCNNNNNNNGG 2 cut(s) 262, 1290
AflIII ACRYGT 2 cut(s) 1761, 2313
AjnI CCWGG 3 cut(s) 1369, 1629, 2372
AjuI GAANNNNNNNTTGG 2 cut(s) 324, 356
AloI GAACNNNNNNTCC 6 cut(s) 165, 197, 378, 410, 1071, 1103
Alw21I GWGCWC 3 cut(s) 349, 681, 1629
Alw26I GTCTC 1 cut(s) 561
Alw44I GTGCAC 2 cut(s) 345, 1625
AlwI GGATC 6 cut(s) 169, 947, 960, 1062, 1371, 1384
Aor51HI AGCGCT 1 cut(s) 469
AoxI GGCC 4 cut(s) 201, 1046, 1226, 1367
ApaLI GTGCAC 2 cut(s) 345, 1625
ApeKI GCWGC 6 cut(s) 299, 479, 1302, 1526, 1655, 1811
ApoI RAATTY 7 cut(s) 820, 829, 1054, 1134, 1330, 1871, 2032
Asp700I GAANNNNTTC 3 cut(s) 393, 969, 1455
AspLEI GCGC 2 cut(s) 470, 986
AspS9I GGNCC 3 cut(s) 68, 202, 1856
AsuHPI GGTGA 2 cut(s) 1142, 1287
AvaII GGWCC 2 cut(s) 68, 1856
BaeGI GKGCMC 3 cut(s) 349, 505, 1629
BamHI GGATCC 2 cut(s) 952, 1376
BanII GRGCYC 2 cut(s) 681, 1021
BbsI GAAGAC 2 cut(s) 2133, 2400
Bbv12I GWGCWC 3 cut(s) 349, 681, 1629
BbvI GCAGC 6 cut(s) 286, 466, 1314, 1513, 1642, 1798
BccI CCATC 3 cut(s) 228, 586, 2356
BciT130I CCWGG 3 cut(s) 1371, 1631, 2374
BclI TGATCA 1 cut(s) 1954
BcoDI GTCTC 1 cut(s) 561
BfaI CTAG 7 cut(s) 152, 383, 813, 1022, 1065, 2072, 2489
BfmI CTRYAG 5 cut(s) 235, 1509, 2079, 2531, 2552
BfoI RGCGCY 1 cut(s) 471
BfuAI ACCTGC 2 cut(s) 295, 712
BglII AGATCT 2 cut(s) 881, 1688
BisI GCNGC 6 cut(s) 300, 480, 1303, 1527, 1656, 1812
BlsI GCNGC 6 cut(s) 301, 481, 1304, 1528, 1657, 1813
Bme1390I CCNGG 3 cut(s) 1371, 1631, 2374
Bme18I GGWCC 2 cut(s) 68, 1856
BmgT120I GGNCC 3 cut(s) 68, 202, 1856
BmiI GGNNCC 4 cut(s) 70, 954, 1378, 1857
BmrFI CCNGG 3 cut(s) 1371, 1631, 2374
BmsI GCATC 7 cut(s) 905, 997, 1576, 1642, 2009, 2128, 2407
BpiI GAAGAC 2 cut(s) 2133, 2400
BplI GAGNNNNNCTC 2 cut(s) 237, 269
BpmI CTGGAG 2 cut(s) 968, 1392
BpuEI CTTGAG 2 cut(s) 1661, 2274
BsaJI CCNNGG 3 cut(s) 834, 1876, 2212
BsaXI ACNNNNNCTCC 6 cut(s) 378, 408, 1071, 1101, 2067, 2097
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 1290
Bse118I RCCGGY 1 cut(s) 1829
Bse1I ACTGG 6 cut(s) 171, 951, 1430, 1504, 1980, 2253
BseBI CCWGG 3 cut(s) 1371, 1631, 2374
BseDI CCNNGG 3 cut(s) 834, 1876, 2212
BseGI GGATG 6 cut(s) 30, 780, 1561, 1591, 1976, 2571
BseLI CCNNNNNNNGG 2 cut(s) 262, 1290
BseMII CTCAG 3 cut(s) 199, 1105, 2327
BseNI ACTGG 6 cut(s) 171, 951, 1430, 1504, 1980, 2253
BseRI GAGGAG 2 cut(s) 946, 1904
BseSI GKGCMC 3 cut(s) 349, 505, 1629
BseXI GCAGC 6 cut(s) 286, 466, 1314, 1513, 1642, 1798
BseYI CCCAGC 3 cut(s) 871, 1298, 2174
BsgI GTGCAG 1 cut(s) 740
BshFI GGCC 4 cut(s) 203, 1048, 1228, 1369
BsiHKAI GWGCWC 3 cut(s) 349, 681, 1629
BsiSI CCGG 1 cut(s) 1830
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 262, 1290
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 4 cut(s) 203, 1048, 1228, 1369
Bsp1286I GDGCHC 5 cut(s) 349, 505, 681, 1021, 1629
Bsp19I CCATGG 1 cut(s) 834
BspACI CCGC 1 cut(s) 392
BspANI GGCC 4 cut(s) 203, 1048, 1228, 1369
BspCNI CTCAG 3 cut(s) 198, 1104, 2328
BspHI TCATGA 1 cut(s) 462
BspLI GGNNCC 4 cut(s) 70, 954, 1378, 1857
BspMI ACCTGC 2 cut(s) 295, 712
BspPI GGATC 6 cut(s) 169, 947, 960, 1062, 1371, 1384
BspQI GCTCTTC 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 1829
BsrI ACTGG 6 cut(s) 171, 951, 1430, 1504, 1980, 2253
BssAI RCCGGY 1 cut(s) 1829
BssECI CCNNGG 3 cut(s) 834, 1876, 2212
BssNAI GTATAC 2 cut(s) 792, 2223
BssT1I CCWWGG 3 cut(s) 834, 1876, 2212
Bst1107I GTATAC 2 cut(s) 792, 2223
Bst2UI CCWGG 3 cut(s) 1371, 1631, 2374
Bst4CI ACNGT 2 cut(s) 1906, 2014
Bst6I CTCTTC 3 cut(s) 669, 1545, 2539
BstAPI GCANNNNNTGC 1 cut(s) 868
BstC8I GCNNGC 2 cut(s) 738, 2159
BstDEI CTNAG 8 cut(s) 18, 43, 185, 305, 1091, 1415, 2336, 2409
BstDSI CCRYGG 1 cut(s) 834
BstEII GGTNACC 2 cut(s) 1293, 2054
BstF5I GGATG 6 cut(s) 30, 780, 1561, 1591, 1976, 2571
BstH2I RGCGCY 1 cut(s) 471
BstHHI GCGC 2 cut(s) 470, 986
BstMAI GTCTC 1 cut(s) 561
BstMWI GCNNNNNNNGC 8 cut(s) 296, 476, 685, 868, 1007, 1016, 1535, 1997
BstNI CCWGG 3 cut(s) 1371, 1631, 2374
BstNSI RCATGY 1 cut(s) 1765
BstPI GGTNACC 2 cut(s) 1293, 2054
BstSCI CCNGG 3 cut(s) 1369, 1629, 2372
BstSFI CTRYAG 5 cut(s) 235, 1509, 2079, 2531, 2552
BstSLI GKGCMC 3 cut(s) 349, 505, 1629
BstV1I GCAGC 6 cut(s) 286, 466, 1314, 1513, 1642, 1798
BstV2I GAAGAC 2 cut(s) 2133, 2400
BstX2I RGATCY 5 cut(s) 881, 952, 1067, 1376, 1688
BstXI CCANNNNNNTGG 2 cut(s) 121, 1797
BstYI RGATCY 5 cut(s) 881, 952, 1067, 1376, 1688
BstZ17I GTATAC 2 cut(s) 792, 2223
BsuRI GGCC 4 cut(s) 203, 1048, 1228, 1369
BtgI CCRYGG 1 cut(s) 834
BtgZI GCGATG 1 cut(s) 2430
BtsCI GGATG 6 cut(s) 30, 780, 1561, 1591, 1976, 2571
BtsI GCAGTG 2 cut(s) 1709, 1986
BtsIMutI CAGTG 7 cut(s) 164, 429, 505, 1437, 1497, 1709, 1986
BveI ACCTGC 2 cut(s) 295, 712
Cac8I GCNNGC 2 cut(s) 738, 2159
CciI TCATGA 1 cut(s) 462
CfoI GCGC 2 cut(s) 470, 986
Cfr10I RCCGGY 1 cut(s) 1829
Cfr13I GGNCC 3 cut(s) 68, 202, 1856
CsiI ACCWGGT 2 cut(s) 1629, 2372
Csp6I GTAC 1 cut(s) 2197
CspCI CAANNNNNGTGG 2 cut(s) 2146, 2181
CviQI GTAC 1 cut(s) 2197
DdeI CTNAG 8 cut(s) 18, 43, 185, 305, 1091, 1415, 2336, 2409
Eam1104I CTCTTC 3 cut(s) 669, 1545, 2539
EarI CTCTTC 3 cut(s) 669, 1545, 2539
Ecl136II GAGCTC 1 cut(s) 679
Eco130I CCWWGG 3 cut(s) 834, 1876, 2212
Eco147I AGGCCT 1 cut(s) 1048
Eco24I GRGCYC 2 cut(s) 681, 1021
Eco32I GATATC 2 cut(s) 1351, 1669
Eco47I GGWCC 2 cut(s) 68, 1856
Eco47III AGCGCT 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 679
Eco57I CTGAAG 3 cut(s) 504, 719, 2145
Eco91I GGTNACC 2 cut(s) 1293, 2054
EcoICRI GAGCTC 1 cut(s) 679
EcoO109I RGGNCCY 1 cut(s) 1856
EcoO65I GGTNACC 2 cut(s) 1293, 2054
EcoRI GAATTC 1 cut(s) 1871
EcoRII CCWGG 3 cut(s) 1369, 1629, 2372
EcoRV GATATC 2 cut(s) 1351, 1669
EcoT14I CCWWGG 3 cut(s) 834, 1876, 2212
EcoT38I GRGCYC 2 cut(s) 681, 1021
ErhI CCWWGG 3 cut(s) 834, 1876, 2212
FalI AAGNNNNNCTT 4 cut(s) 1798, 1830, 2337, 2369
FaqI GGGAC 1 cut(s) 54
FauNDI CATATG 2 cut(s) 1364, 1959
FbaI TGATCA 1 cut(s) 1954
FblI GTMKAC 3 cut(s) 791, 2222, 2574
Fnu4HI GCNGC 6 cut(s) 300, 480, 1303, 1527, 1656, 1812
FokI GGATG 6 cut(s) 17, 787, 1568, 1598, 1963, 2578
FriOI GRGCYC 2 cut(s) 681, 1021
Fsp4HI GCNGC 6 cut(s) 300, 480, 1303, 1527, 1656, 1812
FspBI CTAG 7 cut(s) 152, 383, 813, 1022, 1065, 2072, 2489
GlaI GCGC 2 cut(s) 469, 985
GluI GCNGC 6 cut(s) 300, 480, 1303, 1527, 1656, 1812
GsaI CCCAGC 3 cut(s) 875, 1302, 2178
GsuI CTGGAG 2 cut(s) 968, 1392
HaeII RGCGCY 1 cut(s) 471
HaeIII GGCC 4 cut(s) 203, 1048, 1228, 1369
HapII CCGG 1 cut(s) 1830
HhaI GCGC 2 cut(s) 470, 986
Hin6I GCGC 2 cut(s) 468, 984
HinP1I GCGC 2 cut(s) 468, 984
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 2351
HpaII CCGG 1 cut(s) 1830
HphI GGTGA 2 cut(s) 1142, 1287
Hpy166II GTNNAC 9 cut(s) 347, 589, 792, 1262, 1627, 2069, 2086, 2223, 2575
Hpy188I TCNGA 7 cut(s) 211, 881, 1094, 1168, 1355, 2337, 2517
Hpy188III TCNNGA 8 cut(s) 152, 463, 924, 974, 1475, 1640, 1672, 1798
Hpy8I GTNNAC 9 cut(s) 347, 589, 792, 1262, 1627, 2069, 2086, 2223, 2575
HpyCH4III ACNGT 2 cut(s) 1906, 2014
HpyCH4IV ACGT 1 cut(s) 2313
HpyF10VI GCNNNNNNNGC 8 cut(s) 296, 476, 685, 868, 1007, 1016, 1535, 1997
HpyF3I CTNAG 8 cut(s) 18, 43, 185, 305, 1091, 1415, 2336, 2409
HpySE526I ACGT 1 cut(s) 2313
HspAI GCGC 2 cut(s) 468, 984
Ksp22I TGATCA 1 cut(s) 1954
LguI GCTCTTC 1 cut(s) 669
LmnI GCTCC 4 cut(s) 684, 1535, 1891, 2075
Lsp1109I GCAGC 6 cut(s) 286, 466, 1314, 1513, 1642, 1798
LweI GCATC 7 cut(s) 905, 997, 1576, 1642, 2009, 2128, 2407
MabI ACCWGGT 2 cut(s) 1629, 2372
MaeI CTAG 7 cut(s) 152, 383, 813, 1022, 1065, 2072, 2489
MaeII ACGT 1 cut(s) 2313
MflI RGATCY 5 cut(s) 881, 952, 1067, 1376, 1688
MhlI GDGCHC 5 cut(s) 349, 505, 681, 1021, 1629
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 3 cut(s) 294, 1642, 2583
MroXI GAANNNNTTC 3 cut(s) 393, 969, 1455
MslI CAYNNNNRTG 5 cut(s) 119, 141, 326, 1962, 2166
MspA1I CMGCKG 4 cut(s) 189, 299, 2123, 2536
MspI CCGG 1 cut(s) 1830
MspR9I CCNGG 3 cut(s) 1371, 1631, 2374
MvaI CCWGG 3 cut(s) 1371, 1631, 2374
MwoI GCNNNNNNNGC 8 cut(s) 296, 476, 685, 868, 1007, 1016, 1535, 1997
NcoI CCATGG 1 cut(s) 834
NdeI CATATG 2 cut(s) 1364, 1959
NlaIV GGNNCC 4 cut(s) 70, 954, 1378, 1857
NmuCI GTSAC 6 cut(s) 46, 726, 802, 1293, 1495, 1972
NspI RCATGY 1 cut(s) 1765
PagI TCATGA 1 cut(s) 462
PaqCI CACCTGC 1 cut(s) 712
PceI AGGCCT 1 cut(s) 1048
PciI ACATGT 1 cut(s) 1761
PciSI GCTCTTC 1 cut(s) 669
PdmI GAANNNNTTC 3 cut(s) 393, 969, 1455
PfeI GAWTC 9 cut(s) 148, 266, 495, 993, 1388, 1437, 2023, 2104, 2518
PkrI GCNGC 6 cut(s) 301, 481, 1304, 1528, 1657, 1813
PleI GAGTC 1 cut(s) 862
PpsI GAGTC 1 cut(s) 862
PpuMI RGGWCCY 1 cut(s) 1856
PscI ACATGT 1 cut(s) 1761
PsiI TTATAA 1 cut(s) 2626
Psp124BI GAGCTC 1 cut(s) 681
Psp5II RGGWCCY 1 cut(s) 1856
Psp6I CCWGG 3 cut(s) 1369, 1629, 2372
PspEI GGTNACC 2 cut(s) 1293, 2054
PspFI CCCAGC 3 cut(s) 871, 1298, 2174
PspGI CCWGG 3 cut(s) 1369, 1629, 2372
PspN4I GGNNCC 4 cut(s) 70, 954, 1378, 1857
PspPI GGNCC 3 cut(s) 68, 202, 1856
PspPPI RGGWCCY 1 cut(s) 1856
PsuI RGATCY 5 cut(s) 881, 952, 1067, 1376, 1688
PvuII CAGCTG 4 cut(s) 189, 299, 2123, 2536
RsaI GTAC 1 cut(s) 2198
RsaNI GTAC 1 cut(s) 2197
RseI CAYNNNNRTG 5 cut(s) 119, 141, 326, 1962, 2166
SacI GAGCTC 1 cut(s) 681
SapI GCTCTTC 1 cut(s) 669
SatI GCNGC 6 cut(s) 300, 480, 1303, 1527, 1656, 1812
Sau96I GGNCC 3 cut(s) 68, 202, 1856
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 3 cut(s) 1371, 1631, 2374
SduI GDGCHC 5 cut(s) 349, 505, 681, 1021, 1629
SexAI ACCWGGT 2 cut(s) 1629, 2372
SfaNI GCATC 7 cut(s) 905, 997, 1576, 1642, 2009, 2128, 2407
SfcI CTRYAG 5 cut(s) 235, 1509, 2079, 2531, 2552
SinI GGWCC 2 cut(s) 68, 1856
SmiMI CAYNNNNRTG 5 cut(s) 119, 141, 326, 1962, 2166
SmlI CTYRAG 2 cut(s) 1640, 2289
SmoI CTYRAG 2 cut(s) 1640, 2289
SseBI AGGCCT 1 cut(s) 1048
SsiI CCGC 1 cut(s) 392
SspI AATATT 3 cut(s) 640, 2095, 2505
SspMI CTAG 7 cut(s) 152, 383, 813, 1022, 1065, 2072, 2489
SstI GAGCTC 1 cut(s) 681
StuI AGGCCT 1 cut(s) 1048
StyD4I CCNGG 3 cut(s) 1369, 1629, 2372
StyI CCWWGG 3 cut(s) 834, 1876, 2212
TaaI ACNGT 2 cut(s) 1906, 2014
TaiI ACGT 1 cut(s) 2316
TaqI TCGA 6 cut(s) 10, 621, 973, 1464, 2318, 2521
TfiI GAWTC 9 cut(s) 148, 266, 495, 993, 1388, 1437, 2023, 2104, 2518
TscAI CASTG 7 cut(s) 171, 436, 505, 1437, 1504, 1716, 1993
TseFI GTSAC 6 cut(s) 46, 726, 802, 1293, 1495, 1972
TseI GCWGC 6 cut(s) 299, 479, 1302, 1526, 1655, 1811
Tsp45I GTSAC 6 cut(s) 46, 726, 802, 1293, 1495, 1972
TspGWI ACGGA 1 cut(s) 2184
TspRI CASTG 7 cut(s) 171, 436, 505, 1437, 1504, 1716, 1993
VneI GTGCAC 2 cut(s) 345, 1625
VpaK11BI GGWCC 2 cut(s) 68, 1856
XapI RAATTY 7 cut(s) 820, 829, 1054, 1134, 1330, 1871, 2032
XbaI TCTAGA 1 cut(s) 151
XceI RCATGY 1 cut(s) 1765
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XmiI GTMKAC 3 cut(s) 791, 2222, 2574
XmnI GAANNNNTTC 3 cut(s) 393, 969, 1455
XspI CTAG 7 cut(s) 152, 383, 813, 1022, 1065, 2072, 2489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.