Belongs to the cullin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 517 |
| AbsI | CCTCGAGG | 1 cut(s) | 69 |
| AciI | CCGC | 1 cut(s) | 683 |
| AclWI | GGATC | 5 cut(s) | 74, 87, 1045, 1145, 1345 |
| AcoI | YGGCCR | 2 cut(s) | 636, 737 |
| AcsI | RAATTY | 2 cut(s) | 806, 881 |
| AcuI | CTGAAG | 2 cut(s) | 138, 593 |
| AcyI | GRCGYC | 1 cut(s) | 514 |
| AfaI | GTAC | 3 cut(s) | 242, 851, 862 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 301, 778, 1060 |
| AflII | CTTAAG | 1 cut(s) | 383 |
| AgeI | ACCGGT | 1 cut(s) | 1156 |
| AgsI | TTSAA | 7 cut(s) | 100, 887, 946, 1108, 1184, 1309, 1321 |
| AjnI | CCWGG | 1 cut(s) | 694 |
| AluBI | AGCT | 5 cut(s) | 229, 342, 505, 744, 904 |
| AluI | AGCT | 5 cut(s) | 229, 342, 505, 744, 904 |
| Alw26I | GTCTC | 2 cut(s) | 422, 1087 |
| AlwI | GGATC | 5 cut(s) | 74, 87, 1045, 1145, 1345 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 716 |
| Ama87I | CYCGRG | 1 cut(s) | 69 |
| AoxI | GGCC | 3 cut(s) | 291, 636, 737 |
| ApeKI | GCWGC | 3 cut(s) | 103, 152, 229 |
| ApoI | RAATTY | 2 cut(s) | 806, 881 |
| AsiGI | ACCGGT | 1 cut(s) | 1156 |
| AspS9I | GGNCC | 2 cut(s) | 292, 475 |
| AvaI | CYCGRG | 1 cut(s) | 69 |
| AvaII | GGWCC | 1 cut(s) | 475 |
| BalI | TGGCCA | 2 cut(s) | 638, 739 |
| BamHI | GGATCC | 1 cut(s) | 79 |
| BbsI | GAAGAC | 1 cut(s) | 509 |
| BbvI | GCAGC | 3 cut(s) | 115, 164, 216 |
| BccI | CCATC | 2 cut(s) | 601, 1220 |
| BceAI | ACGGC | 1 cut(s) | 1230 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1280, 1314 |
| BciT130I | CCWGG | 1 cut(s) | 696 |
| BcoDI | GTCTC | 2 cut(s) | 422, 1087 |
| BfaI | CTAG | 2 cut(s) | 39, 345 |
| BfmI | CTRYAG | 1 cut(s) | 493 |
| BfrI | CTTAAG | 1 cut(s) | 383 |
| BglII | AGATCT | 1 cut(s) | 312 |
| BisI | GCNGC | 3 cut(s) | 104, 153, 230 |
| BlsI | GCNGC | 3 cut(s) | 105, 154, 231 |
| Bme1390I | CCNGG | 1 cut(s) | 696 |
| Bme18I | GGWCC | 1 cut(s) | 475 |
| BmeT110I | CYCGRG | 1 cut(s) | 69 |
| BmgT120I | GGNCC | 2 cut(s) | 292, 475 |
| BmiI | GGNNCC | 2 cut(s) | 81, 1065 |
| BmrFI | CCNGG | 1 cut(s) | 696 |
| BmrI | ACTGGG | 1 cut(s) | 866 |
| BmsI | GCATC | 3 cut(s) | 139, 1207, 1229 |
| BmuI | ACTGGG | 1 cut(s) | 866 |
| BpiI | GAAGAC | 1 cut(s) | 509 |
| BpuEI | CTTGAG | 1 cut(s) | 455 |
| Bsa29I | ATCGAT | 2 cut(s) | 1215, 1340 |
| BsaHI | GRCGYC | 1 cut(s) | 514 |
| BsaJI | CCNNGG | 2 cut(s) | 639, 695 |
| BsaWI | WCCGGW | 1 cut(s) | 1156 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 301, 778, 1060 |
| Bse118I | RCCGGY | 1 cut(s) | 1156 |
| Bse1I | ACTGG | 3 cut(s) | 144, 733, 861 |
| BseBI | CCWGG | 1 cut(s) | 696 |
| BseCI | ATCGAT | 2 cut(s) | 1215, 1340 |
| BseDI | CCNNGG | 2 cut(s) | 639, 695 |
| BseGI | GGATG | 4 cut(s) | 10, 34, 187, 852 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 301, 778, 1060 |
| BseMII | CTCAG | 3 cut(s) | 477, 953, 966 |
| BseNI | ACTGG | 3 cut(s) | 144, 733, 861 |
| BseRI | GAGGAG | 2 cut(s) | 440, 677 |
| BseXI | GCAGC | 3 cut(s) | 115, 164, 216 |
| BsgI | GTGCAG | 1 cut(s) | 369 |
| BshFI | GGCC | 3 cut(s) | 293, 638, 739 |
| BshTI | ACCGGT | 1 cut(s) | 1156 |
| BshVI | ATCGAT | 2 cut(s) | 1215, 1340 |
| BsiHKCI | CYCGRG | 1 cut(s) | 69 |
| BsiSI | CCGG | 1 cut(s) | 1157 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 301, 778, 1060 |
| BsmAI | GTCTC | 2 cut(s) | 422, 1087 |
| BsmI | GAATGC | 1 cut(s) | 23 |
| BsnI | GGCC | 3 cut(s) | 293, 638, 739 |
| BsoBI | CYCGRG | 1 cut(s) | 69 |
| Bsp143I | GATC | 7 cut(s) | 31, 79, 312, 1050, 1137, 1337, 1341 |
| BspACI | CCGC | 1 cut(s) | 683 |
| BspANI | GGCC | 3 cut(s) | 293, 638, 739 |
| BspCNI | CTCAG | 3 cut(s) | 478, 954, 965 |
| BspDI | ATCGAT | 2 cut(s) | 1215, 1340 |
| BspLI | GGNNCC | 2 cut(s) | 81, 1065 |
| BspPI | GGATC | 5 cut(s) | 74, 87, 1045, 1145, 1345 |
| BspQI | GCTCTTC | 1 cut(s) | 1235 |
| BspTI | CTTAAG | 1 cut(s) | 383 |
| BsrFI | RCCGGY | 1 cut(s) | 1156 |
| BsrI | ACTGG | 3 cut(s) | 144, 733, 861 |
| BssAI | RCCGGY | 1 cut(s) | 1156 |
| BssECI | CCNNGG | 2 cut(s) | 639, 695 |
| BssMI | GATC | 7 cut(s) | 31, 79, 312, 1050, 1137, 1337, 1341 |
| BssNI | GRCGYC | 1 cut(s) | 514 |
| BssT1I | CCWWGG | 1 cut(s) | 639 |
| Bst2UI | CCWGG | 1 cut(s) | 696 |
| Bst4CI | ACNGT | 4 cut(s) | 712, 718, 1081, 1206 |
| Bst6I | CTCTTC | 1 cut(s) | 1235 |
| BstACI | GRCGYC | 1 cut(s) | 514 |
| BstAFI | CTTAAG | 1 cut(s) | 383 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 149 |
| BstC8I | GCNNGC | 4 cut(s) | 157, 456, 507, 687 |
| BstDEI | CTNAG | 6 cut(s) | 60, 130, 486, 952, 962, 1113 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 299, 1058 |
| BstF5I | GGATG | 4 cut(s) | 10, 34, 187, 852 |
| BstKTI | GATC | 7 cut(s) | 34, 82, 315, 1053, 1140, 1340, 1344 |
| BstMAI | GTCTC | 2 cut(s) | 422, 1087 |
| BstMBI | GATC | 7 cut(s) | 31, 79, 312, 1050, 1137, 1337, 1341 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 149, 1217 |
| BstNI | CCWGG | 1 cut(s) | 696 |
| BstNSI | RCATGY | 2 cut(s) | 110, 1306 |
| BstSCI | CCNGG | 1 cut(s) | 694 |
| BstSFI | CTRYAG | 1 cut(s) | 493 |
| BstV1I | GCAGC | 3 cut(s) | 115, 164, 216 |
| BstV2I | GAAGAC | 1 cut(s) | 509 |
| BstX2I | RGATCY | 3 cut(s) | 79, 312, 1050 |
| BstYI | RGATCY | 3 cut(s) | 79, 312, 1050 |
| Bsu15I | ATCGAT | 2 cut(s) | 1215, 1340 |
| BsuRI | GGCC | 3 cut(s) | 293, 638, 739 |
| BsuTUI | ATCGAT | 2 cut(s) | 1215, 1340 |
| BtsCI | GGATG | 4 cut(s) | 10, 34, 187, 852 |
| BtsIMutI | CAGTG | 2 cut(s) | 726, 854 |
| Cac8I | GCNNGC | 4 cut(s) | 157, 456, 507, 687 |
| CaiI | CAGNNNCTG | 1 cut(s) | 716 |
| Cfr10I | RCCGGY | 1 cut(s) | 1156 |
| Cfr13I | GGNCC | 2 cut(s) | 292, 475 |
| ClaI | ATCGAT | 2 cut(s) | 1215, 1340 |
| Csp6I | GTAC | 3 cut(s) | 241, 850, 861 |
| CspAI | ACCGGT | 1 cut(s) | 1156 |
| CviAII | CATG | 6 cut(s) | 107, 233, 548, 843, 1035, 1303 |
| CviQI | GTAC | 3 cut(s) | 241, 850, 861 |
| DdeI | CTNAG | 6 cut(s) | 60, 130, 486, 952, 962, 1113 |
| DpnI | GATC | 7 cut(s) | 33, 81, 314, 1052, 1139, 1339, 1343 |
| DpnII | GATC | 7 cut(s) | 31, 79, 312, 1050, 1137, 1337, 1341 |
| DraI | TTTAAA | 1 cut(s) | 630 |
| EaeI | YGGCCR | 2 cut(s) | 636, 737 |
| Eam1104I | CTCTTC | 1 cut(s) | 1235 |
| EarI | CTCTTC | 1 cut(s) | 1235 |
| Eco130I | CCWWGG | 1 cut(s) | 639 |
| Eco47I | GGWCC | 1 cut(s) | 475 |
| Eco57I | CTGAAG | 2 cut(s) | 138, 593 |
| Eco88I | CYCGRG | 1 cut(s) | 69 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 299, 1058 |
| EcoO109I | RGGNCCY | 2 cut(s) | 292, 475 |
| EcoRI | GAATTC | 1 cut(s) | 806 |
| EcoRII | CCWGG | 1 cut(s) | 694 |
| EcoT14I | CCWWGG | 1 cut(s) | 639 |
| EcoT22I | ATGCAT | 2 cut(s) | 23, 1222 |
| ErhI | CCWWGG | 1 cut(s) | 639 |
| FaeI | CATG | 6 cut(s) | 110, 236, 551, 846, 1038, 1306 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1301, 1333 |
| FatI | CATG | 6 cut(s) | 106, 232, 547, 842, 1034, 1302 |
| Fnu4HI | GCNGC | 3 cut(s) | 104, 153, 230 |
| FokI | GGATG | 4 cut(s) | 17, 41, 194, 859 |
| Fsp4HI | GCNGC | 3 cut(s) | 104, 153, 230 |
| FspBI | CTAG | 2 cut(s) | 39, 345 |
| GluI | GCNGC | 3 cut(s) | 104, 153, 230 |
| HaeIII | GGCC | 3 cut(s) | 293, 638, 739 |
| HapII | CCGG | 1 cut(s) | 1157 |
| Hin1I | GRCGYC | 1 cut(s) | 514 |
| Hin1II | CATG | 6 cut(s) | 110, 236, 551, 846, 1038, 1306 |
| HincII | GTYRAC | 2 cut(s) | 723, 1192 |
| HindII | GTYRAC | 2 cut(s) | 723, 1192 |
| HindIII | AAGCTT | 1 cut(s) | 503 |
| HinfI | GANTC | 2 cut(s) | 982, 1144 |
| HpaII | CCGG | 1 cut(s) | 1157 |
| Hpy166II | GTNNAC | 5 cut(s) | 597, 723, 1119, 1162, 1192 |
| Hpy188I | TCNGA | 4 cut(s) | 672, 955, 973, 1016 |
| Hpy188III | TCNNGA | 2 cut(s) | 39, 1141 |
| Hpy8I | GTNNAC | 5 cut(s) | 597, 723, 1119, 1162, 1192 |
| Hpy99I | CGWCG | 1 cut(s) | 516 |
| HpyAV | CCTTC | 3 cut(s) | 113, 160, 433 |
| HpyCH4III | ACNGT | 4 cut(s) | 712, 718, 1081, 1206 |
| HpyCH4IV | ACGT | 2 cut(s) | 252, 514 |
| HpyCH4V | TGCA | 7 cut(s) | 21, 152, 196, 232, 321, 350, 1220 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 149, 1217 |
| HpyF3I | CTNAG | 6 cut(s) | 60, 130, 486, 952, 962, 1113 |
| HpySE526I | ACGT | 2 cut(s) | 252, 514 |
| Hsp92I | GRCGYC | 1 cut(s) | 514 |
| Hsp92II | CATG | 6 cut(s) | 110, 236, 551, 846, 1038, 1306 |
| Kzo9I | GATC | 7 cut(s) | 31, 79, 312, 1050, 1137, 1337, 1341 |
| LguI | GCTCTTC | 1 cut(s) | 1235 |
| Lsp1109I | GCAGC | 3 cut(s) | 115, 164, 216 |
| LweI | GCATC | 3 cut(s) | 139, 1207, 1229 |
| MaeI | CTAG | 2 cut(s) | 39, 345 |
| MaeII | ACGT | 2 cut(s) | 252, 514 |
| MaeIII | GTNAC | 6 cut(s) | 109, 467, 619, 712, 910, 965 |
| MalI | GATC | 7 cut(s) | 33, 81, 314, 1052, 1139, 1339, 1343 |
| MboI | GATC | 7 cut(s) | 31, 79, 312, 1050, 1137, 1337, 1341 |
| MboII | GAAGA | 7 cut(s) | 26, 158, 509, 1180, 1183, 1196, 1252 |
| MfeI | CAATTG | 1 cut(s) | 1224 |
| MflI | RGATCY | 3 cut(s) | 79, 312, 1050 |
| MlsI | TGGCCA | 2 cut(s) | 638, 739 |
| MluCI | AATT | 5 cut(s) | 632, 806, 881, 1179, 1224 |
| MluNI | TGGCCA | 2 cut(s) | 638, 739 |
| MlyI | GAGTC | 1 cut(s) | 976 |
| Mox20I | TGGCCA | 2 cut(s) | 638, 739 |
| Mph1103I | ATGCAT | 2 cut(s) | 23, 1222 |
| MscI | TGGCCA | 2 cut(s) | 638, 739 |
| MseI | TTAA | 6 cut(s) | 384, 407, 629, 783, 989, 1056 |
| Msp20I | TGGCCA | 2 cut(s) | 638, 739 |
| MspCI | CTTAAG | 1 cut(s) | 383 |
| MspI | CCGG | 1 cut(s) | 1157 |
| MspR9I | CCNGG | 1 cut(s) | 696 |
| MunI | CAATTG | 1 cut(s) | 1224 |
| Mva1269I | GAATGC | 1 cut(s) | 23 |
| MvaI | CCWGG | 1 cut(s) | 696 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 149, 1217 |
| NdeII | GATC | 7 cut(s) | 31, 79, 312, 1050, 1137, 1337, 1341 |
| NlaIII | CATG | 6 cut(s) | 110, 236, 551, 846, 1038, 1306 |
| NlaIV | GGNNCC | 2 cut(s) | 81, 1065 |
| NmuCI | GTSAC | 2 cut(s) | 467, 910 |
| NsiI | ATGCAT | 2 cut(s) | 23, 1222 |
| NspI | RCATGY | 2 cut(s) | 110, 1306 |
| PaeR7I | CTCGAG | 1 cut(s) | 69 |
| PciSI | GCTCTTC | 1 cut(s) | 1235 |
| PctI | GAATGC | 1 cut(s) | 23 |
| PfeI | GAWTC | 1 cut(s) | 1144 |
| PinAI | ACCGGT | 1 cut(s) | 1156 |
| PkrI | GCNGC | 3 cut(s) | 105, 154, 231 |
| PleI | GAGTC | 1 cut(s) | 976 |
| PpsI | GAGTC | 1 cut(s) | 976 |
| PpuMI | RGGWCCY | 1 cut(s) | 475 |
| Psp5II | RGGWCCY | 1 cut(s) | 475 |
| Psp6I | CCWGG | 1 cut(s) | 694 |
| PspGI | CCWGG | 1 cut(s) | 694 |
| PspN4I | GGNNCC | 2 cut(s) | 81, 1065 |
| PspPI | GGNCC | 2 cut(s) | 292, 475 |
| PspPPI | RGGWCCY | 1 cut(s) | 475 |
| PspXI | VCTCGAGB | 1 cut(s) | 69 |
| PstNI | CAGNNNCTG | 1 cut(s) | 716 |
| PsuI | RGATCY | 3 cut(s) | 79, 312, 1050 |
| RsaI | GTAC | 3 cut(s) | 242, 851, 862 |
| RsaNI | GTAC | 3 cut(s) | 241, 850, 861 |
| SapI | GCTCTTC | 1 cut(s) | 1235 |
| SaqAI | TTAA | 6 cut(s) | 384, 407, 629, 783, 989, 1056 |
| SatI | GCNGC | 3 cut(s) | 104, 153, 230 |
| Sau3AI | GATC | 7 cut(s) | 31, 79, 312, 1050, 1137, 1337, 1341 |
| Sau96I | GGNCC | 2 cut(s) | 292, 475 |
| SchI | GAGTC | 1 cut(s) | 976 |
| ScrFI | CCNGG | 1 cut(s) | 696 |
| SfaNI | GCATC | 3 cut(s) | 139, 1207, 1229 |
| SfcI | CTRYAG | 1 cut(s) | 493 |
| Sfr274I | CTCGAG | 1 cut(s) | 69 |
| SinI | GGWCC | 1 cut(s) | 475 |
| SlaI | CTCGAG | 1 cut(s) | 69 |
| SmlI | CTYRAG | 3 cut(s) | 69, 383, 434 |
| SmoI | CTYRAG | 3 cut(s) | 69, 383, 434 |
| Sse9I | AATT | 5 cut(s) | 632, 806, 881, 1179, 1224 |
| SsiI | CCGC | 1 cut(s) | 683 |
| SspI | AATATT | 1 cut(s) | 96 |
| SspMI | CTAG | 2 cut(s) | 39, 345 |
| StyD4I | CCNGG | 1 cut(s) | 694 |
| StyI | CCWWGG | 1 cut(s) | 639 |
| TaaI | ACNGT | 4 cut(s) | 712, 718, 1081, 1206 |
| TaiI | ACGT | 2 cut(s) | 255, 517 |
| TaqI | TCGA | 4 cut(s) | 70, 511, 1215, 1340 |
| TasI | AATT | 5 cut(s) | 632, 806, 881, 1179, 1224 |
| TatI | WGTACW | 2 cut(s) | 240, 849 |
| TfiI | GAWTC | 1 cut(s) | 1144 |
| Tru1I | TTAA | 6 cut(s) | 384, 407, 629, 783, 989, 1056 |
| Tru9I | TTAA | 6 cut(s) | 384, 407, 629, 783, 989, 1056 |
| TscAI | CASTG | 2 cut(s) | 733, 861 |
| TseFI | GTSAC | 2 cut(s) | 467, 910 |
| TseI | GCWGC | 3 cut(s) | 103, 152, 229 |
| Tsp45I | GTSAC | 2 cut(s) | 467, 910 |
| TspDTI | ATGAA | 4 cut(s) | 21, 273, 429, 1253 |
| TspGWI | ACGGA | 1 cut(s) | 26 |
| TspRI | CASTG | 2 cut(s) | 733, 861 |
| Vha464I | CTTAAG | 1 cut(s) | 383 |
| VpaK11BI | GGWCC | 1 cut(s) | 475 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 299, 1058 |
| XapI | RAATTY | 2 cut(s) | 806, 881 |
| XbaI | TCTAGA | 1 cut(s) | 38 |
| XceI | RCATGY | 2 cut(s) | 110, 1306 |
| XhoI | CTCGAG | 1 cut(s) | 69 |
| XspI | CTAG | 2 cut(s) | 39, 345 |
| ZraI | GACGTC | 1 cut(s) | 515 |
| Zsp2I | ATGCAT | 2 cut(s) | 23, 1222 |