RLG00000021680

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000084
Physical Location & Seq
Forward (+)
77231827 .. 77239835
8009 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021680

Sequence Viewer

Length: 2109 bp
ATGGAGGGGTTGAGAAGAGGGTTTGGGTCTGGTTCAGCTATGGCGGCTGTGGTGCTTTTGGCCTTGGTTTTGGTAATGGTTCCAGAAGCCTCAGCTTCTCGTATTACTGTGGGAGGTAACAAGGGCTGGTCTCCCAATGTTAACTACACAATTTGGGCGCAAGACAAGCACTTCTACAATGGAGATTGGCTCTTTTTTGTGTATGATAGGAACCAGCAGAATATCCTGGAGGTGAACAAGACAAACTATGAGACATGCAATTCAGATCATCCACTTCACAATTGGACTACAGGAGTTGGAAGAGATGTGGTTCCACTAAATGTGACCAGGCACTACTATTTCTTGAGTGGCAATGGCTTCTGCTACAGTGGCATGAAGATGGCTATTCAGGTTGAAAAAATACCTCCACCTCCCAAGGCTGCTCCGGTGGTGAGCAAGGGCAGTGCTCCATTAGCGCCTGTTTTTAGATCCCAATTTGTGTTGCCAGCCATTTTTGCTATTGGTGCAGTTTGGGATGCATTTCTTAGCACGATAAAGGTAGACAAAATTCAACTTTCTTATTGCTGTTCAACTATGGACAGCCGTGGAATAATAGCTAAGGTTGAGCCCAAGAATAAGGCTTCAATTGATAAATTACCGTCTTGTATTCTGTCACACGTACTTTCATTTTTACCAATAAAAGATGCCGTGGATACTTGCTTGTTATCCCAGCAGTGGAGGCACCTTTGGAAGCATCCAATACTGACGAAGCCCAATCTTGAATTTGACATTCTAAACATTTTTGGACATAATTATGCTCAACTCGTTGAAGAATATGAAGCATACAAATTCAATCCCGTTTTTATGCGTCAACTCGAGAGACAAAGCTCTCTGTTTGTGAGACGTGTGAATGAAATTTTGCTGCTCTACAATCGTAAGAAGGTAGACTCTTTCAAAGTGGCATACTCTTTCGATGCAGACTCTACGGTCATCCTTGATGAATGGGTTCGTTTTGCAATTACAAGGGGAGTTGAAGTACTCGATATTCAATTATCAAGCCGCAATGGTGTTTTGGATACAGAAAATGTTTATGTTTTTCCCCACTGGCTCCTTTCAGAATTAAATGCATCAACTCTAAGGCATTTGTCGTTGCAGAGATGCATTCTAAAACCTCCTACTGATTTCGACATATTCATTTGGCTAAGAACGCTTTGTCTAAACAAGGTTATTGTTGATCCAGCTTTTTTGGCACATATGTTCTCTGTATGTTCGCTCCTCGAAAGCTTGACCTTGAGCTATTGCAGAGGGGGCTCATGTCTAATTGTTGGTCCTTCACTTCGTCTTAATGATCTTAAAGTTCTCAGGTGTGTCGGTTTAAAAAGTGTAGAGATTGATGCAGTAAACCTTTCTTCTTTTGAATATTGTGGATTACATTCGATAATTTCTTGCATGAGAACTCCACAGCTAGTAAGATGTTTTTTTAAGGGATGTTCTTTTAAGGGATGTTCTGAAGGAGGAATATCTTATGCGCTCACCCAACTTGCCTCCTGTCCTGGGCTTGAGACTCTTCATCTGCAGAGCTATTATAATGAAGATATACCTCAAACTGTTTTGACATTGAGAAACCTGAAACAAGTGAACTTGGATCTCTTCATGGGAAACTTCAACCTTGGGAGTGTTCTGAATATTCTCAAGGCCGCACCTCTTTTAGAAGAGTTTATAATAACGGTACGAGCAACAAGCTATCAAGGAGATGTAAGGAATCTTTCGGGATTTTCACATGACCATTTGAGAAGATTTAAGATGCAGGGGTTCCAGGGTAAGTGGATCGAGATAGAATTGGCAATTAGCATTCTTAAAATTGCAACAAATCTCGAGGTATTGGTGATTGATCCATATGGAAAATATTACGATGGTGGTGGAAGCTGGATCGAAATCGACTGTTGTTATTACAGGGAGGGGTATGAGAATGAGGGCAAGGATGACAATGAAGAGAAAATTTTCCTGCACATAGAGGACAGTGTGGATGTGGTGGATTTGCAGTACTTCTGGTGGAAACAAAGAGGTAGTGTGGTTGTCCGAAAAAGGCTGGAGGAAGTAATGACTGATGCTCAAGTTGTAATCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

703

Amino Acids

79.58

Weight (kDa)

6.76

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 43 - 125 2.2e-21 Plastocyanin-like domain
F-box PF00646 209 - 249 1.7e-06 F-box domain
LRR_At1g61320_AtMIF1 PF23622 314 - 629 9.2e-48 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 325 - 464 3.8e-07 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000084)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29034
fragaria_vesca FvH4_2g19762 FvH4_3g09170 FvH4_5g20951 FvH4_6g26050 FvH4_6g34920 FvH4_6g44020 FvH4_6g44041 FvH4_6g44050 FvH4_6g47821 FvH4_6g47821 FvH4_6g47970 FvH4_6g48151 FvH4_6g48180 FvH4_6g48300 FvH4_6g48320 FvH4_6g48330 FvH4_6g48330 FvH4_6g48330 FvH4_6g48431 FvH4_6g50821 FvH4_6g50840 FvH4_7g25361 FvH4_7g27481 FvH4_7g31230
malus_domestica MD02G1023100.v1.1 MD09G1053300.v1.1 MD09G1063900.v1.1 MD15G1165800.v1.1 MD15G1165900.v1.1
prunus_persica Prupe.3G259300_v2.0.a1 Prupe.3G259800_v2.0.a1 Prupe.3G265300_v2.0.a1 Prupe.5G213700_v2.0.a1 Prupe.7G131300_v2.0.a1 Prupe.7G249000_v2.0.a1 Prupe.7G249000_v2.0.a1 Prupe.7G249000_v2.0.a1
pyrus_communis pycom02g02000 pycom02g02010 pycom111g05350 pycom15g14860
rosa_chinensis RchiOBHm_Chr1g0366891 RchiOBHm_Chr2g0087591 RchiOBHm_Chr2g0162581 RchiOBHm_Chr2g0162591 RchiOBHm_Chr2g0166621 RchiOBHm_Chr2g0167501 RchiOBHm_Chr2g0167541 RchiOBHm_Chr2g0167551 RchiOBHm_Chr2g0167561 RchiOBHm_Chr2g0167591 RchiOBHm_Chr2g0167601 RchiOBHm_Chr2g0167641 RchiOBHm_Chr2g0167681 RchiOBHm_Chr2g0168251 RchiOBHm_Chr3g0476161 RchiOBHm_Chr3g0476181 RchiOBHm_Chr3g0476211 RchiOBHm_Chr5g0008761 RchiOBHm_Chr5g0047501 RchiOBHm_Chr6g0269121
rosa_laevigata RLG00000011477 RLG00000014026 RLG00000015309 RLG00000015881 RLG00000017161 RLG00000018247 RLG00000019905 RLG00000021634 RLG00000021635 RLG00000021680 RLG00000021681 RLG00000021725 RLG00000021734 RLG00000021735 RLG00000021739 RLG00000021741 RLG00000021745 RLG00000021756 RLG00000021758 RLG00000021759 RLG00000022096 RLG00000023339 RLG00000023786 RLG00000025547 RLG00000031523 RLG00000031601 RLG00000031628 RLG00000033737 RLG00000034477 RLG00000034478
rosa_multiflora Rmu_co8440045.1_g000001 Rmu_co8505525.1_g000001 Rmu_sc0000141.1_g000004 Rmu_sc0000148.1_g000068 Rmu_sc0000258.1_g000040 Rmu_sc0000272.1_g000035 Rmu_sc0000309.1_g000036 Rmu_sc0000335.1_g000109 Rmu_sc0000335.1_g000110 Rmu_sc0000382.1_g000008 Rmu_sc0000902.1_g000005 Rmu_sc0001392.1_g000007 Rmu_sc0001665.1_g000048 Rmu_sc0001833.1_g000013 Rmu_sc0001970.1_g000001 Rmu_sc0001970.1_g000006 Rmu_sc0001970.1_g000013 Rmu_sc0002034.1_g000008 Rmu_sc0002106.1_g000008 Rmu_sc0002268.1_g000017 Rmu_sc0002299.1_g000026 Rmu_sc0002329.1_g000037 Rmu_sc0002755.1_g000002 Rmu_sc0003264.1_g000024 Rmu_sc0003354.1_g000004 Rmu_sc0003433.1_g000010 Rmu_sc0003511.1_g000007 Rmu_sc0004888.1_g000041 Rmu_sc0006599.1_g000001 Rmu_sc0006763.1_g000016 Rmu_sc0006763.1_g000017 Rmu_sc0007003.1_g000002 Rmu_sc0007418.1_g000028 Rmu_sc0008222.1_g000002 Rmu_sc0010971.1_g000001 Rmu_sc0011091.1_g000001 Rmu_sc0011091.1_g000002 Rmu_sc0013199.1_g000005 Rmu_sc0016089.1_g000001 Rmu_sc0024549.1_g000004 Rmu_sc0024939.1_g000001 Rmu_sc0025855.1_g000003 Rmu_sc0026912.1_g000001 Rmu_sc0039772.1_g000001 Rmu_ssc0000171.1_g000028 Rmu_ssc0000171.1_g000030
rosa_roxburghii Rroxscaffold_1G00067230 Rroxscaffold_1G00075140 Rroxscaffold_2G00080050 Rroxscaffold_2G00083230 Rroxscaffold_2G00083240 Rroxscaffold_2G00083340 Rroxscaffold_2G00083390 Rroxscaffold_2G00083400 Rroxscaffold_2G00083420 Rroxscaffold_2G00083430 Rroxscaffold_2G00083940 Rroxscaffold_2G00084400 Rroxscaffold_2G00084700 Rroxscaffold_2G00084710 Rroxscaffold_2G00102510 Rroxscaffold_2G00105780 Rroxscaffold_2G00125110 Rroxscaffold_2G00125750 Rroxscaffold_2G00125790 Rroxscaffold_2G00153440 Rroxscaffold_3G00259540 Rroxscaffold_4G00314070 Rroxscaffold_5G00345290 Rroxscaffold_6G00399110 Rroxscaffold_6G00430120
rosa_rugosa Rorug01G0086600 Rorug01G0476800 Rorug02G0530700 Rorug03G0121000 Rorug03G0153000 Rorug03G0272400 Rorug03G0279800 Rorug04G0438800 Rorug05G0566700 Rorug05G0566800 Rorug06G0114400 Rorug07G0057400 Rorug07G0073700
rosa_samantha Rh1AG028900 Rh1AG062000 Rh1AG108900 Rh1AG183100 Rh1BG072000 Rh2AG030200 Rh2AG400600 Rh2AG420900 Rh2AG534000 Rh2AG590500 Rh2AG598300 Rh2AG598700 Rh2AG599000 Rh2AG599100 Rh2AG599500 Rh2AG599600 Rh2AG599900 Rh2AG600200 Rh2AG601200 Rh2AG633000 Rh2BG030200 Rh2BG097100 Rh2BG318200 Rh2BG403500 Rh2BG577200 Rh2BG602600 Rh2BG602700 Rh2BG609600 Rh2BG609700 Rh2BG610200 Rh2BG610400 Rh2BG610700 Rh2BG614300 Rh2BG646600 Rh2CG030500 Rh2CG099200 Rh2CG283200 Rh2CG386900 Rh2CG573500 Rh2CG573600 Rh2CG580400 Rh2CG580500 Rh2CG580600 Rh2CG580800 Rh2CG581100 Rh2CG582100 Rh2CG613300 Rh2CG613400 Rh2DG030400 Rh2DG613100 Rh2DG613200 Rh2DG621400 Rh2DG621600 Rh2DG621800 Rh2DG622500 Rh2DG622800 Rh2DG623100 Rh2DG623400 Rh2DG624500 Rh2DG624600 Rh2DG624800 Rh2DG661200 Rh2DG661300 Rh3AG048300 Rh3AG177800 Rh3BG203700 Rh3BG234500 Rh3BG234700 Rh3BG235200 Rh3BG330000 Rh3CG049600 Rh3CG194100 Rh3CG201200 Rh3CG230000 Rh3CG350600 Rh3DG050400 Rh3DG171400 Rh3DG304300 Rh4BG006600 Rh4BG159000 Rh4CG126300 Rh4CG171700 Rh4CG241000 Rh4DG007800 Rh4DG042200 Rh4DG311700 Rh5AG067200 Rh5AG070100 Rh5BG067000 Rh5BG247800 Rh5BG324200 Rh5BG443300 Rh5CG074800 Rh5CG078200 Rh5CG078300 Rh5CG180800 Rh5DG061800 Rh5DG066300 Rh5DG066400 Rh5DG167500 Rh5DG335000 Rh6AG174900 Rh6CG174500 Rh6CG392000 Rh6DG167000 Rh7BG185800 Rh7DG175300
rosa_wichuraiana Rw0G012810 Rw0G014220 Rw0G014230 Rw0G014240 Rw2G002410 Rw2G049760 Rw2G049770 Rw2G049780 Rw2G049800 Rw2G049820 Rw2G049920 Rw4G000180 Rw4G001290 Rw4G003630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1566, 1700
AasI GACNNNNNNGTC 1 cut(s) 965
AccB1I GGYRCC 1 cut(s) 720
AccI GTMKAC 2 cut(s) 540, 924
AciI CCGC 3 cut(s) 44, 1039, 1677
AclWI GGATC 6 cut(s) 462, 1208, 1632, 1814, 1865, 1916
AcsI RAATTY 5 cut(s) 546, 761, 827, 894, 1977
AcuI CTGAAG 1 cut(s) 1509
AfaI GTAC 4 cut(s) 660, 1017, 1710, 2024
AfiI CCNNNNNNNGG 2 cut(s) 714, 1533
AflIII ACRYGT 2 cut(s) 655, 883
AjiI CACGTC 1 cut(s) 884
AjnI CCWGG 4 cut(s) 225, 326, 1531, 1794
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 5 cut(s) 135, 245, 853, 874, 1535
AlwI GGATC 6 cut(s) 462, 1208, 1632, 1814, 1865, 1916
Ama87I CYCGRG 2 cut(s) 854, 1853
AoxI GGCC 2 cut(s) 60, 1674
ApeKI GCWGC 2 cut(s) 419, 901
ApoI RAATTY 5 cut(s) 546, 761, 827, 894, 1977
Asp700I GAANNNNTTC 2 cut(s) 984, 1979
AspLEI GCGC 3 cut(s) 160, 457, 1510
AspS9I GGNCC 1 cut(s) 1307
AsuHPI GGTGA 4 cut(s) 244, 442, 1504, 1876
AvaI CYCGRG 2 cut(s) 854, 1853
AvaII GGWCC 1 cut(s) 1307
BanI GGYRCC 1 cut(s) 720
BanII GRGCYC 2 cut(s) 609, 1292
Bbv12I GWGCWC 1 cut(s) 448
BbvCI CCTCAGC 1 cut(s) 91
BbvI GCAGC 2 cut(s) 406, 888
BccI CCATC 2 cut(s) 373, 1886
BceAI ACGGC 2 cut(s) 567, 671
BciT130I CCWGG 4 cut(s) 227, 328, 1533, 1796
BciVI GTATCC 2 cut(s) 685, 1048
BcoDI GTCTC 5 cut(s) 135, 245, 853, 874, 1535
BfaI CTAG 1 cut(s) 1445
BfmI CTRYAG 3 cut(s) 288, 364, 1553
BfoI RGCGCY 1 cut(s) 458
BfuI GTATCC 2 cut(s) 685, 1048
BisI GCNGC 5 cut(s) 45, 420, 902, 1039, 1677
BlsI GCNGC 5 cut(s) 46, 421, 903, 1040, 1678
BmcAI AGTACT 2 cut(s) 1017, 2024
Bme1390I CCNGG 4 cut(s) 227, 328, 1533, 1796
Bme18I GGWCC 1 cut(s) 1307
BmeT110I CYCGRG 2 cut(s) 854, 1853
BmgBI CACGTC 1 cut(s) 884
BmgT120I GGNCC 1 cut(s) 1307
BmiI GGNNCC 6 cut(s) 81, 212, 312, 722, 1088, 1793
BmrFI CCNGG 4 cut(s) 227, 328, 1533, 1796
BmsI GCATC 9 cut(s) 505, 673, 742, 943, 1115, 1127, 1363, 1773, 2077
BplI GAGNNNNNCTC 2 cut(s) 174, 206
BpmI CTGGAG 2 cut(s) 248, 2090
Bpu10I CCTNAGC 2 cut(s) 91, 597
BpuEI CTTGAG 5 cut(s) 364, 1291, 1559, 1655, 2076
BsaAI YACGTR 1 cut(s) 658
BsaBI GATNNNNATC 1 cut(s) 1913
BsaI GGTCTC 1 cut(s) 135
BsaJI CCNNGG 7 cut(s) 63, 414, 583, 687, 1532, 1648, 1795
BsaWI WCCGGW 1 cut(s) 424
BsaXI ACNNNNNCTCC 2 cut(s) 999, 1029
Bsc4I CCNNNNNNNGG 2 cut(s) 714, 1533
Bse1I ACTGG 1 cut(s) 1088
Bse3DI GCAATG 2 cut(s) 358, 1048
Bse8I GATNNNNATC 1 cut(s) 1913
BseBI CCWGG 4 cut(s) 227, 328, 1533, 1796
BseDI CCNNGG 7 cut(s) 63, 414, 583, 687, 1532, 1648, 1795
BseGI GGATG 8 cut(s) 268, 520, 733, 969, 1472, 1487, 1966, 2011
BseJI GATNNNNATC 1 cut(s) 1913
BseLI CCNNNNNNNGG 2 cut(s) 714, 1533
BseMI GCAATG 2 cut(s) 358, 1048
BseMII CTCAG 2 cut(s) 105, 1354
BseNI ACTGG 1 cut(s) 1088
BseRI GAGGAG 1 cut(s) 1244
BseXI GCAGC 2 cut(s) 406, 888
BseYI CCCAGC 1 cut(s) 708
BsgI GTGCAG 2 cut(s) 525, 1970
BshFI GGCC 2 cut(s) 62, 1676
BshNI GGYRCC 1 cut(s) 720
BsiHKAI GWGCWC 1 cut(s) 448
BsiHKCI CYCGRG 2 cut(s) 854, 1853
BsiSI CCGG 1 cut(s) 425
BslI CCNNNNNNNGG 2 cut(s) 714, 1533
BsmAI GTCTC 5 cut(s) 135, 245, 853, 874, 1535
BsmBI CGTCTC 1 cut(s) 874
BsmI GAATGC 2 cut(s) 1140, 1830
BsnI GGCC 2 cut(s) 62, 1676
Bso31I GGTCTC 1 cut(s) 135
BsoBI CYCGRG 2 cut(s) 854, 1853
Bsp1286I GDGCHC 3 cut(s) 448, 609, 1292
Bsp143I GATC 8 cut(s) 265, 467, 1213, 1327, 1624, 1806, 1870, 1908
BspACI CCGC 3 cut(s) 44, 1039, 1677
BspANI GGCC 2 cut(s) 62, 1676
BspCNI CTCAG 2 cut(s) 104, 1353
BspLI GGNNCC 6 cut(s) 81, 212, 312, 722, 1088, 1793
BspMAI CTGCAG 1 cut(s) 1557
BspPI GGATC 6 cut(s) 462, 1208, 1632, 1814, 1865, 1916
BspT107I GGYRCC 1 cut(s) 720
BspTNI GGTCTC 1 cut(s) 135
BsrDI GCAATG 2 cut(s) 358, 1048
BsrI ACTGG 1 cut(s) 1088
BssECI CCNNGG 7 cut(s) 63, 414, 583, 687, 1532, 1648, 1795
BssMI GATC 8 cut(s) 265, 467, 1213, 1327, 1624, 1806, 1870, 1908
BssT1I CCWWGG 3 cut(s) 63, 414, 1648
Bst2UI CCWGG 4 cut(s) 227, 328, 1533, 1796
Bst4CI ACNGT 8 cut(s) 109, 368, 639, 967, 1588, 1708, 1922, 2000
Bst6I CTCTTC 6 cut(s) 10, 295, 1551, 1634, 1686, 1965
BstBAI YACGTR 1 cut(s) 658
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 6 cut(s) 91, 524, 597, 1115, 1181, 1340
BstDSI CCRYGG 2 cut(s) 583, 687
BstF5I GGATG 8 cut(s) 268, 520, 733, 969, 1472, 1487, 1966, 2011
BstH2I RGCGCY 1 cut(s) 458
BstHHI GCGC 3 cut(s) 160, 457, 1510
BstKTI GATC 8 cut(s) 268, 470, 1216, 1330, 1627, 1809, 1873, 1911
BstMAI GTCTC 5 cut(s) 135, 245, 853, 874, 1535
BstMBI GATC 8 cut(s) 265, 467, 1213, 1327, 1624, 1806, 1870, 1908
BstNI CCWGG 4 cut(s) 227, 328, 1533, 1796
BstNSI RCATGY 1 cut(s) 258
BstSCI CCNGG 4 cut(s) 225, 326, 1531, 1794
BstSFI CTRYAG 3 cut(s) 288, 364, 1553
BstV1I GCAGC 2 cut(s) 406, 888
BstX2I RGATCY 2 cut(s) 467, 1624
BstYI RGATCY 2 cut(s) 467, 1624
BsuI GTATCC 2 cut(s) 685, 1048
BsuRI GGCC 2 cut(s) 62, 1676
BtgI CCRYGG 2 cut(s) 583, 687
BtrI CACGTC 1 cut(s) 884
BtsCI GGATG 8 cut(s) 268, 520, 733, 969, 1472, 1487, 1966, 2011
BtsI GCAGTG 2 cut(s) 448, 719
BtsIMutI CAGTG 5 cut(s) 373, 448, 719, 1081, 2005
Cac8I GCNNGC 1 cut(s) 486
CfoI GCGC 3 cut(s) 160, 457, 1510
Cfr13I GGNCC 1 cut(s) 1307
CseI GACGC 1 cut(s) 836
Csp6I GTAC 4 cut(s) 659, 1016, 1709, 2023
CviAII CATG 6 cut(s) 255, 373, 1293, 1429, 1633, 1760
CviQI GTAC 4 cut(s) 659, 1016, 1709, 2023
DdeI CTNAG 6 cut(s) 91, 524, 597, 1115, 1181, 1340
DpnI GATC 8 cut(s) 267, 469, 1215, 1329, 1626, 1808, 1872, 1910
DpnII GATC 8 cut(s) 265, 467, 1213, 1327, 1624, 1806, 1870, 1908
DraI TTTAAA 1 cut(s) 1356
DrdI GACNNNNNNGTC 1 cut(s) 965
DseDI GACNNNNNNGTC 1 cut(s) 965
Eam1104I CTCTTC 6 cut(s) 10, 295, 1551, 1634, 1686, 1965
EarI CTCTTC 6 cut(s) 10, 295, 1551, 1634, 1686, 1965
Eco130I CCWWGG 3 cut(s) 63, 414, 1648
Eco24I GRGCYC 2 cut(s) 609, 1292
Eco31I GGTCTC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 1307
Eco57I CTGAAG 1 cut(s) 1509
Eco88I CYCGRG 2 cut(s) 854, 1853
EcoRII CCWGG 4 cut(s) 225, 326, 1531, 1794
EcoT14I CCWWGG 3 cut(s) 63, 414, 1648
EcoT22I ATGCAT 3 cut(s) 520, 1108, 1142
EcoT38I GRGCYC 2 cut(s) 609, 1292
ErhI CCWWGG 3 cut(s) 63, 414, 1648
Esp3I CGTCTC 1 cut(s) 874
FaeI CATG 6 cut(s) 258, 376, 1296, 1432, 1636, 1763
FatI CATG 6 cut(s) 254, 372, 1292, 1428, 1632, 1759
FauNDI CATATG 2 cut(s) 1233, 1876
FblI GTMKAC 2 cut(s) 540, 924
Fnu4HI GCNGC 5 cut(s) 45, 420, 902, 1039, 1677
FokI GGATG 8 cut(s) 255, 527, 720, 956, 1479, 1494, 1973, 2018
FriOI GRGCYC 2 cut(s) 609, 1292
Fsp4HI GCNGC 5 cut(s) 45, 420, 902, 1039, 1677
FspBI CTAG 1 cut(s) 1445
GlaI GCGC 3 cut(s) 159, 456, 1509
GluI GCNGC 5 cut(s) 45, 420, 902, 1039, 1677
GsaI CCCAGC 1 cut(s) 712
GsuI CTGGAG 2 cut(s) 248, 2090
HaeII RGCGCY 1 cut(s) 458
HaeIII GGCC 2 cut(s) 62, 1676
HapII CCGG 1 cut(s) 425
HgaI GACGC 1 cut(s) 836
HhaI GCGC 3 cut(s) 160, 457, 1510
Hin1II CATG 6 cut(s) 258, 376, 1296, 1432, 1636, 1763
Hin6I GCGC 3 cut(s) 158, 455, 1508
HinP1I GCGC 3 cut(s) 158, 455, 1508
HincII GTYRAC 2 cut(s) 142, 851
HindII GTYRAC 2 cut(s) 142, 851
HindIII AAGCTT 1 cut(s) 1261
HinfI GANTC 4 cut(s) 926, 959, 1543, 1741
HpaI GTTAAC 1 cut(s) 142
HpaII CCGG 1 cut(s) 425
HphI GGTGA 4 cut(s) 244, 442, 1504, 1876
Hpy166II GTNNAC 7 cut(s) 142, 235, 541, 851, 925, 1381, 1618
Hpy188I TCNGA 5 cut(s) 265, 1096, 1489, 1662, 2060
Hpy188III TCNNGA 7 cut(s) 83, 343, 758, 856, 1749, 1810, 1853
Hpy8I GTNNAC 7 cut(s) 142, 235, 541, 851, 925, 1381, 1618
HpyAV CCTTC 3 cut(s) 913, 1320, 1484
HpyCH4III ACNGT 8 cut(s) 109, 368, 639, 967, 1588, 1708, 1922, 2000
HpyCH4IV ACGT 2 cut(s) 657, 883
HpyF3I CTNAG 6 cut(s) 91, 524, 597, 1115, 1181, 1340
HpySE526I ACGT 2 cut(s) 657, 883
Hsp92II CATG 6 cut(s) 258, 376, 1296, 1432, 1636, 1763
HspAI GCGC 3 cut(s) 158, 455, 1508
KspAI GTTAAC 1 cut(s) 142
Kzo9I GATC 8 cut(s) 265, 467, 1213, 1327, 1624, 1806, 1870, 1908
LmnI GCTCC 4 cut(s) 427, 451, 1092, 1257
Lsp1109I GCAGC 2 cut(s) 406, 888
LweI GCATC 9 cut(s) 505, 673, 742, 943, 1115, 1127, 1363, 1773, 2077
MaeI CTAG 1 cut(s) 1445
MaeII ACGT 2 cut(s) 657, 883
MaeIII GTNAC 3 cut(s) 116, 322, 651
MalI GATC 8 cut(s) 267, 469, 1215, 1329, 1626, 1808, 1872, 1910
MboI GATC 8 cut(s) 265, 467, 1213, 1327, 1624, 1806, 1870, 1908
MfeI CAATTG 2 cut(s) 280, 624
MflI RGATCY 2 cut(s) 467, 1624
MhlI GDGCHC 3 cut(s) 448, 609, 1292
MlyI GAGTC 3 cut(s) 920, 953, 1537
MmeI TCCRAC 1 cut(s) 277
Mph1103I ATGCAT 3 cut(s) 520, 1108, 1142
MroXI GAANNNNTTC 2 cut(s) 984, 1979
MseI TTAA 9 cut(s) 141, 1100, 1323, 1332, 1355, 1461, 1476, 1779, 1836
MslI CAYNNNNRTG 2 cut(s) 377, 792
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 4 cut(s) 227, 328, 1533, 1796
MunI CAATTG 2 cut(s) 280, 624
Mva1269I GAATGC 2 cut(s) 1140, 1830
MvaI CCWGG 4 cut(s) 227, 328, 1533, 1796
NdeI CATATG 2 cut(s) 1233, 1876
NdeII GATC 8 cut(s) 265, 467, 1213, 1327, 1624, 1806, 1870, 1908
NlaIII CATG 6 cut(s) 258, 376, 1296, 1432, 1636, 1763
NlaIV GGNNCC 6 cut(s) 81, 212, 312, 722, 1088, 1793
NmuCI GTSAC 2 cut(s) 322, 651
NsiI ATGCAT 3 cut(s) 520, 1108, 1142
NspI RCATGY 1 cut(s) 258
PaeR7I CTCGAG 2 cut(s) 854, 1853
PctI GAATGC 2 cut(s) 1140, 1830
PdmI GAANNNNTTC 2 cut(s) 984, 1979
PfeI GAWTC 1 cut(s) 1741
PfoI TCCNGGA 1 cut(s) 225
PkrI GCNGC 5 cut(s) 46, 421, 903, 1040, 1678
PleI GAGTC 3 cut(s) 920, 953, 1537
PpsI GAGTC 3 cut(s) 920, 953, 1537
Ppu21I YACGTR 1 cut(s) 658
PsiI TTATAA 2 cut(s) 1566, 1700
Psp6I CCWGG 4 cut(s) 225, 326, 1531, 1794
PspFI CCCAGC 1 cut(s) 708
PspGI CCWGG 4 cut(s) 225, 326, 1531, 1794
PspN4I GGNNCC 6 cut(s) 81, 212, 312, 722, 1088, 1793
PspPI GGNCC 1 cut(s) 1307
PstI CTGCAG 1 cut(s) 1557
PsuI RGATCY 2 cut(s) 467, 1624
RsaI GTAC 4 cut(s) 660, 1017, 1710, 2024
RsaNI GTAC 4 cut(s) 659, 1016, 1709, 2023
RseI CAYNNNNRTG 2 cut(s) 377, 792
SaqAI TTAA 9 cut(s) 141, 1100, 1323, 1332, 1355, 1461, 1476, 1779, 1836
SatI GCNGC 5 cut(s) 45, 420, 902, 1039, 1677
Sau3AI GATC 8 cut(s) 265, 467, 1213, 1327, 1624, 1806, 1870, 1908
Sau96I GGNCC 1 cut(s) 1307
ScaI AGTACT 2 cut(s) 1017, 2024
SchI GAGTC 3 cut(s) 920, 953, 1537
ScrFI CCNGG 4 cut(s) 227, 328, 1533, 1796
SduI GDGCHC 3 cut(s) 448, 609, 1292
SfaNI GCATC 9 cut(s) 505, 673, 742, 943, 1115, 1127, 1363, 1773, 2077
SfcI CTRYAG 3 cut(s) 288, 364, 1553
Sfr274I CTCGAG 2 cut(s) 854, 1853
SinI GGWCC 1 cut(s) 1307
SlaI CTCGAG 2 cut(s) 854, 1853
SmiMI CAYNNNNRTG 2 cut(s) 377, 792
SmlI CTYRAG 7 cut(s) 343, 854, 1270, 1538, 1670, 1853, 2091
SmoI CTYRAG 7 cut(s) 343, 854, 1270, 1538, 1670, 1853, 2091
SsiI CCGC 3 cut(s) 44, 1039, 1677
SspI AATATT 3 cut(s) 1400, 1666, 1886
SspMI CTAG 1 cut(s) 1445
StyD4I CCNGG 4 cut(s) 225, 326, 1531, 1794
StyI CCWWGG 3 cut(s) 63, 414, 1648
TaaI ACNGT 8 cut(s) 109, 368, 639, 967, 1588, 1708, 1922, 2000
TaiI ACGT 2 cut(s) 660, 886
TatI WGTACW 2 cut(s) 1015, 2022
TauI GCSGC 3 cut(s) 47, 1041, 1679
TfiI GAWTC 1 cut(s) 1741
Tru1I TTAA 9 cut(s) 141, 1100, 1323, 1332, 1355, 1461, 1476, 1779, 1836
Tru9I TTAA 9 cut(s) 141, 1100, 1323, 1332, 1355, 1461, 1476, 1779, 1836
TscAI CASTG 5 cut(s) 373, 448, 719, 1088, 2005
TseFI GTSAC 2 cut(s) 322, 651
TseI GCWGC 2 cut(s) 419, 901
Tsp45I GTSAC 2 cut(s) 322, 651
TspRI CASTG 5 cut(s) 373, 448, 719, 1088, 2005
VpaK11BI GGWCC 1 cut(s) 1307
XapI RAATTY 5 cut(s) 546, 761, 827, 894, 1977
XceI RCATGY 1 cut(s) 258
XcmI CCANNNNNNNNNTGG 1 cut(s) 279
XhoI CTCGAG 2 cut(s) 854, 1853
XmiI GTMKAC 2 cut(s) 540, 924
XmnI GAANNNNTTC 2 cut(s) 984, 1979
XspI CTAG 1 cut(s) 1445
ZrmI AGTACT 2 cut(s) 1017, 2024
Zsp2I ATGCAT 3 cut(s) 520, 1108, 1142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.