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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 711 |
| AccI | GTMKAC | 1 cut(s) | 222 |
| AciI | CCGC | 1 cut(s) | 850 |
| AclWI | GGATC | 1 cut(s) | 485 |
| AcsI | RAATTY | 1 cut(s) | 756 |
| AfaI | GTAC | 1 cut(s) | 334 |
| AflIII | ACRYGT | 1 cut(s) | 181 |
| AgsI | TTSAA | 6 cut(s) | 118, 127, 232, 311, 443, 451 |
| AjiI | CACGTC | 1 cut(s) | 182 |
| AjnI | CCWGG | 2 cut(s) | 51, 98 |
| AluBI | AGCT | 2 cut(s) | 316, 540 |
| AluI | AGCT | 2 cut(s) | 316, 540 |
| Alw26I | GTCTC | 3 cut(s) | 19, 155, 830 |
| AlwI | GGATC | 1 cut(s) | 485 |
| AoxI | GGCC | 1 cut(s) | 373 |
| ApeKI | GCWGC | 5 cut(s) | 199, 251, 558, 561, 879 |
| ApoI | RAATTY | 1 cut(s) | 756 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 315 |
| AspLEI | GCGC | 1 cut(s) | 105 |
| AspS9I | GGNCC | 1 cut(s) | 374 |
| AsuC2I | CCSGG | 1 cut(s) | 829 |
| AsuHPI | GGTGA | 2 cut(s) | 350, 614 |
| BbvI | GCAGC | 4 cut(s) | 211, 238, 545, 548 |
| BccI | CCATC | 3 cut(s) | 252, 496, 769 |
| BciT130I | CCWGG | 2 cut(s) | 53, 100 |
| BcnI | CCSGG | 1 cut(s) | 829 |
| BcoDI | GTCTC | 3 cut(s) | 19, 155, 830 |
| BfaI | CTAG | 2 cut(s) | 110, 533 |
| BfmI | CTRYAG | 2 cut(s) | 252, 718 |
| BfuAI | ACCTGC | 1 cut(s) | 711 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 683 |
| BisI | GCNGC | 5 cut(s) | 200, 252, 559, 562, 880 |
| BlsI | GCNGC | 5 cut(s) | 201, 253, 560, 563, 881 |
| Bme1390I | CCNGG | 3 cut(s) | 53, 100, 829 |
| BmgBI | CACGTC | 1 cut(s) | 182 |
| BmgT120I | GGNCC | 1 cut(s) | 374 |
| BmiI | GGNNCC | 1 cut(s) | 646 |
| BmrFI | CCNGG | 3 cut(s) | 53, 100, 829 |
| BmsI | GCATC | 1 cut(s) | 843 |
| BpuEI | CTTGAG | 2 cut(s) | 783, 854 |
| BpuMI | CCSGG | 1 cut(s) | 829 |
| BsaBI | GATNNNNATC | 1 cut(s) | 495 |
| BsaJI | CCNNGG | 3 cut(s) | 681, 743, 827 |
| Bse3DI | GCAATG | 1 cut(s) | 245 |
| Bse8I | GATNNNNATC | 1 cut(s) | 495 |
| BseBI | CCWGG | 2 cut(s) | 53, 100 |
| BseDI | CCNNGG | 3 cut(s) | 681, 743, 827 |
| BseGI | GGATG | 2 cut(s) | 85, 858 |
| BseJI | GATNNNNATC | 1 cut(s) | 495 |
| BseMI | GCAATG | 1 cut(s) | 245 |
| BseMII | CTCAG | 1 cut(s) | 80 |
| BseRI | GAGGAG | 2 cut(s) | 54, 173 |
| BseXI | GCAGC | 4 cut(s) | 211, 238, 545, 548 |
| BsgI | GTGCAG | 1 cut(s) | 547 |
| BshFI | GGCC | 1 cut(s) | 375 |
| BsiSI | CCGG | 1 cut(s) | 829 |
| BslFI | GGGAC | 1 cut(s) | 810 |
| BsmAI | GTCTC | 3 cut(s) | 19, 155, 830 |
| BsmFI | GGGAC | 1 cut(s) | 810 |
| BsnI | GGCC | 1 cut(s) | 375 |
| Bsp143I | GATC | 3 cut(s) | 490, 604, 616 |
| BspACI | CCGC | 1 cut(s) | 850 |
| BspANI | GGCC | 1 cut(s) | 375 |
| BspCNI | CTCAG | 1 cut(s) | 79 |
| BspLI | GGNNCC | 1 cut(s) | 646 |
| BspMAI | CTGCAG | 2 cut(s) | 256, 722 |
| BspMI | ACCTGC | 1 cut(s) | 711 |
| BspPI | GGATC | 1 cut(s) | 485 |
| BsrDI | GCAATG | 1 cut(s) | 245 |
| BssECI | CCNNGG | 3 cut(s) | 681, 743, 827 |
| BssMI | GATC | 3 cut(s) | 490, 604, 616 |
| BssT1I | CCWWGG | 2 cut(s) | 681, 743 |
| Bst2UI | CCWGG | 2 cut(s) | 53, 100 |
| Bst4CI | ACNGT | 3 cut(s) | 362, 575, 635 |
| Bst6I | CTCTTC | 3 cut(s) | 471, 518, 846 |
| BstC8I | GCNNGC | 1 cut(s) | 858 |
| BstDEI | CTNAG | 2 cut(s) | 66, 419 |
| BstF5I | GGATG | 2 cut(s) | 85, 858 |
| BstHHI | GCGC | 1 cut(s) | 105 |
| BstKTI | GATC | 3 cut(s) | 493, 607, 619 |
| BstMAI | GTCTC | 3 cut(s) | 19, 155, 830 |
| BstMBI | GATC | 3 cut(s) | 490, 604, 616 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 683, 717 |
| BstNI | CCWGG | 2 cut(s) | 53, 100 |
| BstSCI | CCNGG | 3 cut(s) | 51, 98, 827 |
| BstSFI | CTRYAG | 2 cut(s) | 252, 718 |
| BstV1I | GCAGC | 4 cut(s) | 211, 238, 545, 548 |
| BsuRI | GGCC | 1 cut(s) | 375 |
| BtrI | CACGTC | 1 cut(s) | 182 |
| BtsCI | GGATG | 2 cut(s) | 85, 858 |
| BtsI | GCAGTG | 1 cut(s) | 45 |
| BtsIMutI | CAGTG | 3 cut(s) | 45, 74, 631 |
| BveI | ACCTGC | 1 cut(s) | 711 |
| Cac8I | GCNNGC | 1 cut(s) | 858 |
| CfoI | GCGC | 1 cut(s) | 105 |
| Cfr13I | GGNCC | 1 cut(s) | 374 |
| CseI | GACGC | 1 cut(s) | 44 |
| Csp6I | GTAC | 1 cut(s) | 333 |
| CviJI | RGCY | 7 cut(s) | 56, 270, 316, 375, 457, 540, 832 |
| CviKI_1 | RGCY | 7 cut(s) | 56, 270, 316, 375, 457, 540, 832 |
| CviQI | GTAC | 1 cut(s) | 333 |
| DdeI | CTNAG | 2 cut(s) | 66, 419 |
| DpnI | GATC | 3 cut(s) | 492, 606, 618 |
| DpnII | GATC | 3 cut(s) | 490, 604, 616 |
| DraI | TTTAAA | 1 cut(s) | 384 |
| Eam1104I | CTCTTC | 3 cut(s) | 471, 518, 846 |
| EarI | CTCTTC | 3 cut(s) | 471, 518, 846 |
| Eco130I | CCWWGG | 2 cut(s) | 681, 743 |
| EcoRII | CCWGG | 2 cut(s) | 51, 98 |
| EcoT14I | CCWWGG | 2 cut(s) | 681, 743 |
| ErhI | CCWWGG | 2 cut(s) | 681, 743 |
| FaiI | YATR | 7 cut(s) | 11, 156, 348, 496, 550, 688, 884 |
| FaqI | GGGAC | 1 cut(s) | 810 |
| FblI | GTMKAC | 1 cut(s) | 222 |
| Fnu4HI | GCNGC | 5 cut(s) | 200, 252, 559, 562, 880 |
| FokI | GGATG | 2 cut(s) | 72, 865 |
| Fsp4HI | GCNGC | 5 cut(s) | 200, 252, 559, 562, 880 |
| FspBI | CTAG | 2 cut(s) | 110, 533 |
| GlaI | GCGC | 1 cut(s) | 104 |
| GluI | GCNGC | 5 cut(s) | 200, 252, 559, 562, 880 |
| HaeIII | GGCC | 1 cut(s) | 375 |
| HapII | CCGG | 1 cut(s) | 829 |
| HgaI | GACGC | 1 cut(s) | 44 |
| HhaI | GCGC | 1 cut(s) | 105 |
| Hin6I | GCGC | 1 cut(s) | 103 |
| HinP1I | GCGC | 1 cut(s) | 103 |
| HincII | GTYRAC | 1 cut(s) | 223 |
| HindII | GTYRAC | 1 cut(s) | 223 |
| HindIII | AAGCTT | 2 cut(s) | 314, 538 |
| HinfI | GANTC | 6 cut(s) | 5, 224, 257, 283, 447, 838 |
| HpaII | CCGG | 1 cut(s) | 829 |
| HphI | GGTGA | 2 cut(s) | 350, 614 |
| Hpy166II | GTNNAC | 3 cut(s) | 223, 365, 581 |
| Hpy188I | TCNGA | 1 cut(s) | 501 |
| Hpy188III | TCNNGA | 2 cut(s) | 110, 753 |
| Hpy8I | GTNNAC | 3 cut(s) | 223, 365, 581 |
| Hpy99I | CGWCG | 1 cut(s) | 154 |
| HpyAV | CCTTC | 1 cut(s) | 437 |
| HpyCH4III | ACNGT | 3 cut(s) | 362, 575, 635 |
| HpyCH4IV | ACGT | 2 cut(s) | 152, 181 |
| HpyCH4V | TGCA | 7 cut(s) | 176, 199, 238, 254, 564, 720, 818 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 683, 717 |
| HpyF3I | CTNAG | 2 cut(s) | 66, 419 |
| HpySE526I | ACGT | 2 cut(s) | 152, 181 |
| HspAI | GCGC | 1 cut(s) | 103 |
| Kzo9I | GATC | 3 cut(s) | 490, 604, 616 |
| LmnI | GCTCC | 1 cut(s) | 138 |
| LpnPI | CCDG | 9 cut(s) | 38, 65, 85, 112, 585, 706, 766, 842, 870 |
| Lsp1109I | GCAGC | 4 cut(s) | 211, 238, 545, 548 |
| LweI | GCATC | 1 cut(s) | 843 |
| MaeI | CTAG | 2 cut(s) | 110, 533 |
| MaeII | ACGT | 2 cut(s) | 152, 181 |
| MaeIII | GTNAC | 2 cut(s) | 212, 627 |
| MalI | GATC | 3 cut(s) | 492, 606, 618 |
| MboI | GATC | 3 cut(s) | 490, 604, 616 |
| MboII | GAAGA | 4 cut(s) | 314, 458, 505, 833 |
| MluCI | AATT | 4 cut(s) | 233, 756, 812, 873 |
| MlyI | GAGTC | 3 cut(s) | 218, 277, 832 |
| MnlI | CCTC | 9 cut(s) | 36, 72, 75, 151, 366, 435, 634, 688, 807 |
| MroXI | GAANNNNTTC | 1 cut(s) | 315 |
| MseI | TTAA | 3 cut(s) | 383, 693, 892 |
| MslI | CAYNNNNRTG | 1 cut(s) | 636 |
| MspI | CCGG | 1 cut(s) | 829 |
| MspR9I | CCNGG | 3 cut(s) | 53, 100, 829 |
| MvaI | CCWGG | 2 cut(s) | 53, 100 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 683, 717 |
| NciI | CCSGG | 1 cut(s) | 829 |
| NdeII | GATC | 3 cut(s) | 490, 604, 616 |
| NlaIV | GGNNCC | 1 cut(s) | 646 |
| PdmI | GAANNNNTTC | 1 cut(s) | 315 |
| PfeI | GAWTC | 3 cut(s) | 5, 257, 447 |
| PkrI | GCNGC | 5 cut(s) | 201, 253, 560, 563, 881 |
| PleI | GAGTC | 3 cut(s) | 218, 277, 832 |
| PpsI | GAGTC | 3 cut(s) | 218, 277, 832 |
| Psp6I | CCWGG | 2 cut(s) | 51, 98 |
| PspGI | CCWGG | 2 cut(s) | 51, 98 |
| PspN4I | GGNNCC | 1 cut(s) | 646 |
| PspPI | GGNCC | 1 cut(s) | 374 |
| PstI | CTGCAG | 2 cut(s) | 256, 722 |
| RsaI | GTAC | 1 cut(s) | 334 |
| RsaNI | GTAC | 1 cut(s) | 333 |
| RseI | CAYNNNNRTG | 1 cut(s) | 636 |
| SalI | GTCGAC | 1 cut(s) | 221 |
| SaqAI | TTAA | 3 cut(s) | 383, 693, 892 |
| SatI | GCNGC | 5 cut(s) | 200, 252, 559, 562, 880 |
| Sau3AI | GATC | 3 cut(s) | 490, 604, 616 |
| Sau96I | GGNCC | 1 cut(s) | 374 |
| SchI | GAGTC | 3 cut(s) | 218, 277, 832 |
| ScrFI | CCNGG | 3 cut(s) | 53, 100, 829 |
| SfaNI | GCATC | 1 cut(s) | 843 |
| SfcI | CTRYAG | 2 cut(s) | 252, 718 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 636 |
| SmlI | CTYRAG | 2 cut(s) | 798, 833 |
| SmoI | CTYRAG | 2 cut(s) | 798, 833 |
| Sse9I | AATT | 4 cut(s) | 233, 756, 812, 873 |
| SsiI | CCGC | 1 cut(s) | 850 |
| SspI | AATATT | 2 cut(s) | 194, 592 |
| SspMI | CTAG | 2 cut(s) | 110, 533 |
| StyD4I | CCNGG | 3 cut(s) | 51, 98, 827 |
| StyI | CCWWGG | 2 cut(s) | 681, 743 |
| TaaI | ACNGT | 3 cut(s) | 362, 575, 635 |
| TaiI | ACGT | 2 cut(s) | 155, 184 |
| TaqI | TCGA | 3 cut(s) | 222, 325, 489 |
| TasI | AATT | 4 cut(s) | 233, 756, 812, 873 |
| TfiI | GAWTC | 3 cut(s) | 5, 257, 447 |
| Tru1I | TTAA | 3 cut(s) | 383, 693, 892 |
| Tru9I | TTAA | 3 cut(s) | 383, 693, 892 |
| TscAI | CASTG | 3 cut(s) | 45, 74, 638 |
| TseI | GCWGC | 5 cut(s) | 199, 251, 558, 561, 879 |
| TspDTI | ATGAA | 4 cut(s) | 291, 308, 458, 833 |
| TspRI | CASTG | 3 cut(s) | 45, 74, 638 |
| XapI | RAATTY | 1 cut(s) | 756 |
| XbaI | TCTAGA | 1 cut(s) | 109 |
| XmiI | GTMKAC | 1 cut(s) | 222 |
| XmnI | GAANNNNTTC | 1 cut(s) | 315 |
| XspI | CTAG | 2 cut(s) | 110, 533 |