Rorug04G0438800

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000084
Physical Location & Seq
Forward (+)
59595517 .. 59597879
2363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0438800.1

Sequence Viewer

Length: 720 bp
ATGGCCAGGAACGGTGTCGTTTCGTGGCGGAGGAGGTCGGACAAGCTCGAAGGCTTGGATCTTTCCAGTTCCGAAGAAGCTCACGTCCTCGCTGTCGATGACAGCCTCGTCGATCGCAAGGTCATTGAGCGCTTGCTCAGAATCTCCTCTTGCAAAGTGACCGCTGTGGACAGTGGTATGAGAGCTCTGCAATTCCTAGGTTTAGACGAGGAGAAGAGCACCTCGGTCGGTTTTGATGGCTTGAAGGTGGATCTGATTATCACGGATTACTGCATGCCTGGTATGACCGGATATGAACTGCTTAAGAAAATCAAGGAATCCACGACTTTTAAAGAGATTCCGGTTGTGATAATGTCCTCTGAGAACGTTTTGGCGCGCATAGACAGGTGTTTGGAAGAAGGGGCAGAAGATTTCATAGTGAAGCCGGTGAAGTTATCGGACGTAAAAAGGCTTAAGGATTACATGACAAAAGACGTCGTTGCCGGAGGAGTAGAAGAGAGTGGGATCAACAAGAGAAAGCTACGAGAACCGTCCGATCAGCCACCGTCTCCGCCGTCAATTTTACCGTCATCTCCAACGTCAGCCCTATCATCACCTTCACCTTCACCGTCACCGTCACCATCACCGCCGCTTTCATCGTCGTCCTCGTTCTCAGCTCCATCGTCGCCCACGTCGCTTGATTCTCCAATCACACGGCTCAAAATGACCAACACTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

239

Amino Acids

25.91

Weight (kDa)

5.45

Isoelectric Point (pI)

78.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Response_reg PF00072 29 - 148 3e-21 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000084)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29034
fragaria_vesca FvH4_2g19762 FvH4_3g09170 FvH4_5g20951 FvH4_6g26050 FvH4_6g34920 FvH4_6g44020 FvH4_6g44041 FvH4_6g44050 FvH4_6g47821 FvH4_6g47821 FvH4_6g47970 FvH4_6g48151 FvH4_6g48180 FvH4_6g48300 FvH4_6g48320 FvH4_6g48330 FvH4_6g48330 FvH4_6g48330 FvH4_6g48431 FvH4_6g50821 FvH4_6g50840 FvH4_7g25361 FvH4_7g27481 FvH4_7g31230
malus_domestica MD02G1023100.v1.1 MD09G1053300.v1.1 MD09G1063900.v1.1 MD15G1165800.v1.1 MD15G1165900.v1.1
prunus_persica Prupe.3G259300_v2.0.a1 Prupe.3G259800_v2.0.a1 Prupe.3G265300_v2.0.a1 Prupe.5G213700_v2.0.a1 Prupe.7G131300_v2.0.a1 Prupe.7G249000_v2.0.a1 Prupe.7G249000_v2.0.a1 Prupe.7G249000_v2.0.a1
pyrus_communis pycom02g02000 pycom02g02010 pycom111g05350 pycom15g14860
rosa_chinensis RchiOBHm_Chr1g0366891 RchiOBHm_Chr2g0087591 RchiOBHm_Chr2g0162581 RchiOBHm_Chr2g0162591 RchiOBHm_Chr2g0166621 RchiOBHm_Chr2g0167501 RchiOBHm_Chr2g0167541 RchiOBHm_Chr2g0167551 RchiOBHm_Chr2g0167561 RchiOBHm_Chr2g0167591 RchiOBHm_Chr2g0167601 RchiOBHm_Chr2g0167641 RchiOBHm_Chr2g0167681 RchiOBHm_Chr2g0168251 RchiOBHm_Chr3g0476161 RchiOBHm_Chr3g0476181 RchiOBHm_Chr3g0476211 RchiOBHm_Chr5g0008761 RchiOBHm_Chr5g0047501 RchiOBHm_Chr6g0269121
rosa_laevigata RLG00000011477 RLG00000014026 RLG00000015309 RLG00000015881 RLG00000017161 RLG00000018247 RLG00000019905 RLG00000021634 RLG00000021635 RLG00000021680 RLG00000021681 RLG00000021725 RLG00000021734 RLG00000021735 RLG00000021739 RLG00000021741 RLG00000021745 RLG00000021756 RLG00000021758 RLG00000021759 RLG00000022096 RLG00000023339 RLG00000023786 RLG00000025547 RLG00000031523 RLG00000031601 RLG00000031628 RLG00000033737 RLG00000034477 RLG00000034478
rosa_multiflora Rmu_co8440045.1_g000001 Rmu_co8505525.1_g000001 Rmu_sc0000141.1_g000004 Rmu_sc0000148.1_g000068 Rmu_sc0000258.1_g000040 Rmu_sc0000272.1_g000035 Rmu_sc0000309.1_g000036 Rmu_sc0000335.1_g000109 Rmu_sc0000335.1_g000110 Rmu_sc0000382.1_g000008 Rmu_sc0000902.1_g000005 Rmu_sc0001392.1_g000007 Rmu_sc0001665.1_g000048 Rmu_sc0001833.1_g000013 Rmu_sc0001970.1_g000001 Rmu_sc0001970.1_g000006 Rmu_sc0001970.1_g000013 Rmu_sc0002034.1_g000008 Rmu_sc0002106.1_g000008 Rmu_sc0002268.1_g000017 Rmu_sc0002299.1_g000026 Rmu_sc0002329.1_g000037 Rmu_sc0002755.1_g000002 Rmu_sc0003264.1_g000024 Rmu_sc0003354.1_g000004 Rmu_sc0003433.1_g000010 Rmu_sc0003511.1_g000007 Rmu_sc0004888.1_g000041 Rmu_sc0006599.1_g000001 Rmu_sc0006763.1_g000016 Rmu_sc0006763.1_g000017 Rmu_sc0007003.1_g000002 Rmu_sc0007418.1_g000028 Rmu_sc0008222.1_g000002 Rmu_sc0010971.1_g000001 Rmu_sc0011091.1_g000001 Rmu_sc0011091.1_g000002 Rmu_sc0013199.1_g000005 Rmu_sc0016089.1_g000001 Rmu_sc0024549.1_g000004 Rmu_sc0024939.1_g000001 Rmu_sc0025855.1_g000003 Rmu_sc0026912.1_g000001 Rmu_sc0039772.1_g000001 Rmu_ssc0000171.1_g000028 Rmu_ssc0000171.1_g000030
rosa_roxburghii Rroxscaffold_1G00067230 Rroxscaffold_1G00075140 Rroxscaffold_2G00080050 Rroxscaffold_2G00083230 Rroxscaffold_2G00083240 Rroxscaffold_2G00083340 Rroxscaffold_2G00083390 Rroxscaffold_2G00083400 Rroxscaffold_2G00083420 Rroxscaffold_2G00083430 Rroxscaffold_2G00083940 Rroxscaffold_2G00084400 Rroxscaffold_2G00084700 Rroxscaffold_2G00084710 Rroxscaffold_2G00102510 Rroxscaffold_2G00105780 Rroxscaffold_2G00125110 Rroxscaffold_2G00125750 Rroxscaffold_2G00125790 Rroxscaffold_2G00153440 Rroxscaffold_3G00259540 Rroxscaffold_4G00314070 Rroxscaffold_5G00345290 Rroxscaffold_6G00399110 Rroxscaffold_6G00430120
rosa_rugosa Rorug01G0086600 Rorug01G0476800 Rorug02G0530700 Rorug03G0121000 Rorug03G0153000 Rorug03G0272400 Rorug03G0279800 Rorug04G0438800 Rorug05G0566700 Rorug05G0566800 Rorug06G0114400 Rorug07G0057400 Rorug07G0073700
rosa_samantha Rh1AG028900 Rh1AG062000 Rh1AG108900 Rh1AG183100 Rh1BG072000 Rh2AG030200 Rh2AG400600 Rh2AG420900 Rh2AG534000 Rh2AG590500 Rh2AG598300 Rh2AG598700 Rh2AG599000 Rh2AG599100 Rh2AG599500 Rh2AG599600 Rh2AG599900 Rh2AG600200 Rh2AG601200 Rh2AG633000 Rh2BG030200 Rh2BG097100 Rh2BG318200 Rh2BG403500 Rh2BG577200 Rh2BG602600 Rh2BG602700 Rh2BG609600 Rh2BG609700 Rh2BG610200 Rh2BG610400 Rh2BG610700 Rh2BG614300 Rh2BG646600 Rh2CG030500 Rh2CG099200 Rh2CG283200 Rh2CG386900 Rh2CG573500 Rh2CG573600 Rh2CG580400 Rh2CG580500 Rh2CG580600 Rh2CG580800 Rh2CG581100 Rh2CG582100 Rh2CG613300 Rh2CG613400 Rh2DG030400 Rh2DG613100 Rh2DG613200 Rh2DG621400 Rh2DG621600 Rh2DG621800 Rh2DG622500 Rh2DG622800 Rh2DG623100 Rh2DG623400 Rh2DG624500 Rh2DG624600 Rh2DG624800 Rh2DG661200 Rh2DG661300 Rh3AG048300 Rh3AG177800 Rh3BG203700 Rh3BG234500 Rh3BG234700 Rh3BG235200 Rh3BG330000 Rh3CG049600 Rh3CG194100 Rh3CG201200 Rh3CG230000 Rh3CG350600 Rh3DG050400 Rh3DG171400 Rh3DG304300 Rh4BG006600 Rh4BG159000 Rh4CG126300 Rh4CG171700 Rh4CG241000 Rh4DG007800 Rh4DG042200 Rh4DG311700 Rh5AG067200 Rh5AG070100 Rh5BG067000 Rh5BG247800 Rh5BG324200 Rh5BG443300 Rh5CG074800 Rh5CG078200 Rh5CG078300 Rh5CG180800 Rh5DG061800 Rh5DG066300 Rh5DG066400 Rh5DG167500 Rh5DG335000 Rh6AG174900 Rh6CG174500 Rh6CG392000 Rh6DG167000 Rh7BG185800 Rh7DG175300
rosa_wichuraiana Rw0G012810 Rw0G014220 Rw0G014230 Rw0G014240 Rw2G002410 Rw2G049760 Rw2G049770 Rw2G049780 Rw2G049800 Rw2G049820 Rw2G049920 Rw4G000180 Rw4G001290 Rw4G003630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AatII GACGTC 1 cut(s) 477
AccII CGCG 1 cut(s) 376
AciI CCGC 5 cut(s) 28, 162, 551, 626, 629
AclI AACGTT 1 cut(s) 366
AclWI GGATC 3 cut(s) 66, 258, 512
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 474
AfeI AGCGCT 1 cut(s) 131
AflII CTTAAG 2 cut(s) 302, 452
AgsI TTSAA 1 cut(s) 244
AjiI CACGTC 2 cut(s) 85, 672
AjnI CCWGG 2 cut(s) 5, 277
AluBI AGCT 5 cut(s) 46, 80, 185, 520, 656
AluI AGCT 5 cut(s) 46, 80, 185, 520, 656
Alw21I GWGCWC 2 cut(s) 187, 221
Alw26I GTCTC 1 cut(s) 552
AlwI GGATC 3 cut(s) 66, 258, 512
Aor51HI AGCGCT 1 cut(s) 131
AoxI GGCC 1 cut(s) 3
AspA2I CCTAGG 1 cut(s) 196
AspLEI GCGC 3 cut(s) 132, 376, 378
AsuHPI GGTGA 7 cut(s) 439, 585, 591, 597, 603, 609, 615
AvrII CCTAGG 1 cut(s) 196
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 187
Bbv12I GWGCWC 2 cut(s) 187, 221
BccI CCATC 3 cut(s) 230, 628, 667
BceAI ACGGC 2 cut(s) 538, 710
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 2 cut(s) 7, 279
BcoDI GTCTC 1 cut(s) 552
BfaI CTAG 1 cut(s) 197
BfoI RGCGCY 1 cut(s) 133
BfrI CTTAAG 2 cut(s) 302, 452
BisI GCNGC 1 cut(s) 629
BlnI CCTAGG 1 cut(s) 196
BlsI GCNGC 1 cut(s) 630
Bme1390I CCNGG 2 cut(s) 7, 279
BmgBI CACGTC 2 cut(s) 85, 672
BmrFI CCNGG 2 cut(s) 7, 279
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 2 cut(s) 196, 222
BsaWI WCCGGW 2 cut(s) 287, 340
Bse118I RCCGGY 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 66
BseBI CCWGG 2 cut(s) 7, 279
BseDI CCNNGG 2 cut(s) 196, 222
BseMII CTCAG 3 cut(s) 151, 351, 666
BseNI ACTGG 1 cut(s) 66
BsePI GCGCGC 1 cut(s) 374
BseRI GAGGAG 4 cut(s) 46, 136, 224, 501
Bsh1236I CGCG 1 cut(s) 376
Bsh1285I CGRYCG 2 cut(s) 115, 228
BshFI GGCC 1 cut(s) 5
BsiEI CGRYCG 2 cut(s) 115, 228
BsiHKAI GWGCWC 2 cut(s) 187, 221
BsiSI CCGG 4 cut(s) 288, 341, 425, 483
BsmAI GTCTC 1 cut(s) 552
BsmBI CGTCTC 1 cut(s) 552
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 2 cut(s) 187, 221
Bsp143I GATC 5 cut(s) 58, 112, 250, 504, 535
BspACI CCGC 5 cut(s) 28, 162, 551, 626, 629
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 150, 352, 665
BspFNI CGCG 1 cut(s) 376
BspPI GGATC 3 cut(s) 66, 258, 512
BspQI GCTCTTC 1 cut(s) 209
BspTI CTTAAG 2 cut(s) 302, 452
BsrFI RCCGGY 1 cut(s) 424
BsrI ACTGG 1 cut(s) 66
BssAI RCCGGY 1 cut(s) 424
BssECI CCNNGG 2 cut(s) 196, 222
BssHII GCGCGC 1 cut(s) 374
BssMI GATC 5 cut(s) 58, 112, 250, 504, 535
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 196
Bst2UI CCWGG 2 cut(s) 7, 279
Bst4CI ACNGT 7 cut(s) 14, 173, 531, 546, 567, 609, 615
Bst6I CTCTTC 2 cut(s) 209, 489
BstACI GRCGYC 1 cut(s) 474
BstAFI CTTAAG 2 cut(s) 302, 452
BstC8I GCNNGC 3 cut(s) 134, 275, 376
BstDEI CTNAG 3 cut(s) 137, 360, 652
BstFNI CGCG 1 cut(s) 376
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 3 cut(s) 132, 376, 378
BstKTI GATC 5 cut(s) 61, 115, 253, 507, 538
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 5 cut(s) 58, 112, 250, 504, 535
BstMCI CGRYCG 2 cut(s) 115, 228
BstMWI GCNNNNNNNGC 1 cut(s) 673
BstNI CCWGG 2 cut(s) 7, 279
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 2 cut(s) 5, 277
BstUI CGCG 1 cut(s) 376
BstX2I RGATCY 2 cut(s) 58, 250
BstYI RGATCY 2 cut(s) 58, 250
BsuRI GGCC 1 cut(s) 5
BtrI CACGTC 2 cut(s) 85, 672
BtsIMutI CAGTG 2 cut(s) 178, 711
Cac8I GCNNGC 3 cut(s) 134, 275, 376
CfoI GCGC 3 cut(s) 132, 376, 378
Cfr10I RCCGGY 1 cut(s) 424
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviAII CATG 2 cut(s) 274, 463
DdeI CTNAG 3 cut(s) 137, 360, 652
DpnI GATC 5 cut(s) 60, 114, 252, 506, 537
DpnII GATC 5 cut(s) 58, 112, 250, 504, 535
DraI TTTAAA 1 cut(s) 331
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 209, 489
EarI CTCTTC 2 cut(s) 209, 489
EciI GGCGGA 2 cut(s) 43, 540
Ecl136II GAGCTC 1 cut(s) 185
Eco130I CCWWGG 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 187
Eco47III AGCGCT 1 cut(s) 131
Eco53kI GAGCTC 1 cut(s) 185
EcoICRI GAGCTC 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 5, 277
EcoT14I CCWWGG 1 cut(s) 196
EcoT38I GRGCYC 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 552
FaeI CATG 2 cut(s) 277, 466
FaiI YATR 7 cut(s) 179, 275, 284, 294, 380, 416, 464
FatI CATG 2 cut(s) 273, 462
Fnu4HI GCNGC 1 cut(s) 629
FriOI GRGCYC 1 cut(s) 187
Fsp4HI GCNGC 1 cut(s) 629
FspBI CTAG 1 cut(s) 197
GlaI GCGC 3 cut(s) 131, 375, 377
GluI GCNGC 1 cut(s) 629
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 1 cut(s) 5
HapII CCGG 4 cut(s) 288, 341, 425, 483
HhaI GCGC 3 cut(s) 132, 376, 378
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 2 cut(s) 277, 466
Hin6I GCGC 3 cut(s) 130, 374, 376
HinP1I GCGC 3 cut(s) 130, 374, 376
HinfI GANTC 4 cut(s) 141, 317, 337, 680
HpaII CCGG 4 cut(s) 288, 341, 425, 483
HphI GGTGA 7 cut(s) 439, 585, 591, 597, 603, 609, 615
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 7 cut(s) 40, 73, 140, 255, 361, 439, 535
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 5 cut(s) 113, 479, 643, 667, 676
HpyAV CCTTC 5 cut(s) 44, 238, 392, 606, 612
HpyCH4III ACNGT 7 cut(s) 14, 173, 531, 546, 567, 609, 615
HpyCH4IV ACGT 6 cut(s) 84, 366, 441, 474, 578, 671
HpyCH4V TGCA 3 cut(s) 153, 190, 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 673
HpyF3I CTNAG 3 cut(s) 137, 360, 652
HpySE526I ACGT 6 cut(s) 84, 366, 441, 474, 578, 671
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 2 cut(s) 277, 466
HspAI GCGC 3 cut(s) 130, 374, 376
Kzo9I GATC 5 cut(s) 58, 112, 250, 504, 535
LguI GCTCTTC 1 cut(s) 209
LmnI GCTCC 1 cut(s) 661
LpnPI CCDG 9 cut(s) 19, 79, 264, 291, 301, 354, 370, 438, 496
MaeI CTAG 1 cut(s) 197
MaeII ACGT 6 cut(s) 84, 366, 441, 474, 578, 671
MaeIII GTNAC 3 cut(s) 157, 609, 615
MalI GATC 5 cut(s) 60, 114, 252, 506, 537
MboI GATC 5 cut(s) 58, 112, 250, 504, 535
MboII GAAGA 5 cut(s) 86, 226, 407, 419, 506
MflI RGATCY 2 cut(s) 58, 250
MhlI GDGCHC 2 cut(s) 187, 221
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 191, 558
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 18, 599
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 303, 330, 453
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 164
MspCI CTTAAG 2 cut(s) 302, 452
MspI CCGG 4 cut(s) 288, 341, 425, 483
MspR9I CCNGG 2 cut(s) 7, 279
MvaI CCWGG 2 cut(s) 7, 279
MvnI CGCG 1 cut(s) 376
MwoI GCNNNNNNNGC 1 cut(s) 673
NdeII GATC 5 cut(s) 58, 112, 250, 504, 535
NlaIII CATG 2 cut(s) 277, 466
NmuCI GTSAC 3 cut(s) 157, 609, 615
NspI RCATGY 1 cut(s) 277
PaeI GCATGC 1 cut(s) 277
PauI GCGCGC 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 209
PcsI WCGNNNNNNNCGW 2 cut(s) 644, 668
PfeI GAWTC 4 cut(s) 141, 317, 337, 680
PkrI GCNGC 1 cut(s) 630
Ple19I CGATCG 1 cut(s) 115
Psp124BI GAGCTC 1 cut(s) 187
Psp1406I AACGTT 1 cut(s) 366
Psp6I CCWGG 2 cut(s) 5, 277
PspGI CCWGG 2 cut(s) 5, 277
PsuI RGATCY 2 cut(s) 58, 250
PteI GCGCGC 1 cut(s) 374
PvuI CGATCG 1 cut(s) 115
SacI GAGCTC 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 209
SaqAI TTAA 3 cut(s) 303, 330, 453
SatI GCNGC 1 cut(s) 629
Sau3AI GATC 5 cut(s) 58, 112, 250, 504, 535
ScrFI CCNGG 2 cut(s) 7, 279
SduI GDGCHC 2 cut(s) 187, 221
SmlI CTYRAG 2 cut(s) 302, 452
SmoI CTYRAG 2 cut(s) 302, 452
SphI GCATGC 1 cut(s) 277
Sse9I AATT 2 cut(s) 191, 558
SsiI CCGC 5 cut(s) 28, 162, 551, 626, 629
SspMI CTAG 1 cut(s) 197
SstI GAGCTC 1 cut(s) 187
StyD4I CCNGG 2 cut(s) 5, 277
StyI CCWWGG 1 cut(s) 196
TaaI ACNGT 7 cut(s) 14, 173, 531, 546, 567, 609, 615
TaiI ACGT 6 cut(s) 87, 369, 444, 477, 581, 674
TaqI TCGA 3 cut(s) 48, 96, 111
TaqII GACCGA 1 cut(s) 214
TasI AATT 2 cut(s) 191, 558
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 4 cut(s) 141, 317, 337, 680
Tru1I TTAA 3 cut(s) 303, 330, 453
Tru9I TTAA 3 cut(s) 303, 330, 453
TscAI CASTG 2 cut(s) 178, 718
TseFI GTSAC 3 cut(s) 157, 609, 615
Tsp45I GTSAC 3 cut(s) 157, 609, 615
TspDTI ATGAA 3 cut(s) 309, 403, 624
TspGWI ACGGA 1 cut(s) 278
TspRI CASTG 2 cut(s) 178, 718
Vha464I CTTAAG 2 cut(s) 302, 452
XceI RCATGY 1 cut(s) 277
XmaJI CCTAGG 1 cut(s) 196
XspI CTAG 1 cut(s) 197
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.