Rh2DG030400

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000084
Physical Location & Seq
Reverse (-)
1856890 .. 1859773
2884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG030400.1

Sequence Viewer

Length: 822 bp
ATGTATGCGTACATGTCAAATCTCGAATTTGATTGGTGTAAGATGCTTGCAGCCAAAAGGGCAGCTAGACGGGTCTCCAATCCAAATAGGGGAGTCTATTGCAGGAAGAATGTGCGTTTTTTGGTGATAAGAATAGATAGATTCCTAACCCGCCATTTGGGCTCGAGGATTGCCTCGTTCAAGGTTCGTTGCTGCCTTAAAGATAAGTATGCTCTTAATATCAATGACTGGATTGATTGTGCTGTCAGAAAGGGTGTCGAAAATCTTGACCTAGCCTTTACATGTGATGACATATCTGAACGAATCAATTGGTCACACATGGGCTATTATCAGTTTCCTACTCATCTCCTTGCAGAGGGTAAAGCATCCAGATTAAGGAATCTTTCCTTGCGTTCATGCATGCTTAGGGTAGATTTTCAGGATCGATTCAGCACACTCTCCACTCTTGTGTTGTGTGATGTATATTTTGAAGGACAAGCTCATCCGCTCATGTTTTGTTCTTGTTTAAAGCTTCAGTCTTTGACATTGCAATCGTGTTTTGGATTAGAAAGATTCAGCATTTCACTCGATTATTTGAAGTCACTGGTAGTGAGAAAGTGCAGAGGTTTAAGTGGTATTGAGCTTAGTGCCCCCAATCTTACAACCTTTTATTGTGAAGGCAATGTGATCAAGATCTCATGCATAAAAGTTCCGAATCTGGTGGAAGTTTATGTTTCACTAGGCGGAATTAGTGTTATACATACTTTTGCTCAGCTTGAGAAAGATCTTCCTAATGTCAAGTCTCTGACAGTGAACAAAAGAAATATACCTCTCAGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

273

Amino Acids

31.41

Weight (kDa)

9.38

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 49 - 266 5.3e-29 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 75 - 233 3e-10 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000084)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29034
fragaria_vesca FvH4_2g19762 FvH4_3g09170 FvH4_5g20951 FvH4_6g26050 FvH4_6g34920 FvH4_6g44020 FvH4_6g44041 FvH4_6g44050 FvH4_6g47821 FvH4_6g47821 FvH4_6g47970 FvH4_6g48151 FvH4_6g48180 FvH4_6g48300 FvH4_6g48320 FvH4_6g48330 FvH4_6g48330 FvH4_6g48330 FvH4_6g48431 FvH4_6g50821 FvH4_6g50840 FvH4_7g25361 FvH4_7g27481 FvH4_7g31230
malus_domestica MD02G1023100.v1.1 MD09G1053300.v1.1 MD09G1063900.v1.1 MD15G1165800.v1.1 MD15G1165900.v1.1
prunus_persica Prupe.3G259300_v2.0.a1 Prupe.3G259800_v2.0.a1 Prupe.3G265300_v2.0.a1 Prupe.5G213700_v2.0.a1 Prupe.7G131300_v2.0.a1 Prupe.7G249000_v2.0.a1 Prupe.7G249000_v2.0.a1 Prupe.7G249000_v2.0.a1
pyrus_communis pycom02g02000 pycom02g02010 pycom111g05350 pycom15g14860
rosa_chinensis RchiOBHm_Chr1g0366891 RchiOBHm_Chr2g0087591 RchiOBHm_Chr2g0162581 RchiOBHm_Chr2g0162591 RchiOBHm_Chr2g0166621 RchiOBHm_Chr2g0167501 RchiOBHm_Chr2g0167541 RchiOBHm_Chr2g0167551 RchiOBHm_Chr2g0167561 RchiOBHm_Chr2g0167591 RchiOBHm_Chr2g0167601 RchiOBHm_Chr2g0167641 RchiOBHm_Chr2g0167681 RchiOBHm_Chr2g0168251 RchiOBHm_Chr3g0476161 RchiOBHm_Chr3g0476181 RchiOBHm_Chr3g0476211 RchiOBHm_Chr5g0008761 RchiOBHm_Chr5g0047501 RchiOBHm_Chr6g0269121
rosa_laevigata RLG00000011477 RLG00000014026 RLG00000015309 RLG00000015881 RLG00000017161 RLG00000018247 RLG00000019905 RLG00000021634 RLG00000021635 RLG00000021680 RLG00000021681 RLG00000021725 RLG00000021734 RLG00000021735 RLG00000021739 RLG00000021741 RLG00000021745 RLG00000021756 RLG00000021758 RLG00000021759 RLG00000022096 RLG00000023339 RLG00000023786 RLG00000025547 RLG00000031523 RLG00000031601 RLG00000031628 RLG00000033737 RLG00000034477 RLG00000034478
rosa_multiflora Rmu_co8440045.1_g000001 Rmu_co8505525.1_g000001 Rmu_sc0000141.1_g000004 Rmu_sc0000148.1_g000068 Rmu_sc0000258.1_g000040 Rmu_sc0000272.1_g000035 Rmu_sc0000309.1_g000036 Rmu_sc0000335.1_g000109 Rmu_sc0000335.1_g000110 Rmu_sc0000382.1_g000008 Rmu_sc0000902.1_g000005 Rmu_sc0001392.1_g000007 Rmu_sc0001665.1_g000048 Rmu_sc0001833.1_g000013 Rmu_sc0001970.1_g000001 Rmu_sc0001970.1_g000006 Rmu_sc0001970.1_g000013 Rmu_sc0002034.1_g000008 Rmu_sc0002106.1_g000008 Rmu_sc0002268.1_g000017 Rmu_sc0002299.1_g000026 Rmu_sc0002329.1_g000037 Rmu_sc0002755.1_g000002 Rmu_sc0003264.1_g000024 Rmu_sc0003354.1_g000004 Rmu_sc0003433.1_g000010 Rmu_sc0003511.1_g000007 Rmu_sc0004888.1_g000041 Rmu_sc0006599.1_g000001 Rmu_sc0006763.1_g000016 Rmu_sc0006763.1_g000017 Rmu_sc0007003.1_g000002 Rmu_sc0007418.1_g000028 Rmu_sc0008222.1_g000002 Rmu_sc0010971.1_g000001 Rmu_sc0011091.1_g000001 Rmu_sc0011091.1_g000002 Rmu_sc0013199.1_g000005 Rmu_sc0016089.1_g000001 Rmu_sc0024549.1_g000004 Rmu_sc0024939.1_g000001 Rmu_sc0025855.1_g000003 Rmu_sc0026912.1_g000001 Rmu_sc0039772.1_g000001 Rmu_ssc0000171.1_g000028 Rmu_ssc0000171.1_g000030
rosa_roxburghii Rroxscaffold_1G00067230 Rroxscaffold_1G00075140 Rroxscaffold_2G00080050 Rroxscaffold_2G00083230 Rroxscaffold_2G00083240 Rroxscaffold_2G00083340 Rroxscaffold_2G00083390 Rroxscaffold_2G00083400 Rroxscaffold_2G00083420 Rroxscaffold_2G00083430 Rroxscaffold_2G00083940 Rroxscaffold_2G00084400 Rroxscaffold_2G00084700 Rroxscaffold_2G00084710 Rroxscaffold_2G00102510 Rroxscaffold_2G00105780 Rroxscaffold_2G00125110 Rroxscaffold_2G00125750 Rroxscaffold_2G00125790 Rroxscaffold_2G00153440 Rroxscaffold_3G00259540 Rroxscaffold_4G00314070 Rroxscaffold_5G00345290 Rroxscaffold_6G00399110 Rroxscaffold_6G00430120
rosa_rugosa Rorug01G0086600 Rorug01G0476800 Rorug02G0530700 Rorug03G0121000 Rorug03G0153000 Rorug03G0272400 Rorug03G0279800 Rorug04G0438800 Rorug05G0566700 Rorug05G0566800 Rorug06G0114400 Rorug07G0057400 Rorug07G0073700
rosa_samantha Rh1AG028900 Rh1AG062000 Rh1AG108900 Rh1AG183100 Rh1BG072000 Rh2AG030200 Rh2AG400600 Rh2AG420900 Rh2AG534000 Rh2AG590500 Rh2AG598300 Rh2AG598700 Rh2AG599000 Rh2AG599100 Rh2AG599500 Rh2AG599600 Rh2AG599900 Rh2AG600200 Rh2AG601200 Rh2AG633000 Rh2BG030200 Rh2BG097100 Rh2BG318200 Rh2BG403500 Rh2BG577200 Rh2BG602600 Rh2BG602700 Rh2BG609600 Rh2BG609700 Rh2BG610200 Rh2BG610400 Rh2BG610700 Rh2BG614300 Rh2BG646600 Rh2CG030500 Rh2CG099200 Rh2CG283200 Rh2CG386900 Rh2CG573500 Rh2CG573600 Rh2CG580400 Rh2CG580500 Rh2CG580600 Rh2CG580800 Rh2CG581100 Rh2CG582100 Rh2CG613300 Rh2CG613400 Rh2DG030400 Rh2DG613100 Rh2DG613200 Rh2DG621400 Rh2DG621600 Rh2DG621800 Rh2DG622500 Rh2DG622800 Rh2DG623100 Rh2DG623400 Rh2DG624500 Rh2DG624600 Rh2DG624800 Rh2DG661200 Rh2DG661300 Rh3AG048300 Rh3AG177800 Rh3BG203700 Rh3BG234500 Rh3BG234700 Rh3BG235200 Rh3BG330000 Rh3CG049600 Rh3CG194100 Rh3CG201200 Rh3CG230000 Rh3CG350600 Rh3DG050400 Rh3DG171400 Rh3DG304300 Rh4BG006600 Rh4BG159000 Rh4CG126300 Rh4CG171700 Rh4CG241000 Rh4DG007800 Rh4DG042200 Rh4DG311700 Rh5AG067200 Rh5AG070100 Rh5BG067000 Rh5BG247800 Rh5BG324200 Rh5BG443300 Rh5CG074800 Rh5CG078200 Rh5CG078300 Rh5CG180800 Rh5DG061800 Rh5DG066300 Rh5DG066400 Rh5DG167500 Rh5DG335000 Rh6AG174900 Rh6CG174500 Rh6CG392000 Rh6DG167000 Rh7BG185800 Rh7DG175300
rosa_wichuraiana Rw0G012810 Rw0G014220 Rw0G014230 Rw0G014240 Rw2G002410 Rw2G049760 Rw2G049770 Rw2G049780 Rw2G049800 Rw2G049820 Rw2G049920 Rw4G000180 Rw4G001290 Rw4G003630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 487
AciI CCGC 3 cut(s) 151, 485, 723
AclWI GGATC 1 cut(s) 429
AcsI RAATTY 1 cut(s) 26
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 4 cut(s) 89, 157, 355, 375
AflIII ACRYGT 2 cut(s) 12, 281
AgsI TTSAA 3 cut(s) 181, 470, 577
AleI CACNNNNGTG 1 cut(s) 446
AluBI AGCT 5 cut(s) 65, 479, 511, 622, 754
AluI AGCT 5 cut(s) 65, 479, 511, 622, 754
Alw26I GTCTC 2 cut(s) 79, 786
AlwI GGATC 1 cut(s) 429
Ama87I CYCGRG 1 cut(s) 163
ApeKI GCWGC 3 cut(s) 50, 62, 192
ApoI RAATTY 1 cut(s) 26
AsuHPI GGTGA 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 631
BanII GRGCYC 1 cut(s) 164
BbvI GCAGC 3 cut(s) 62, 74, 179
BccI CCATC 1 cut(s) 810
BcgI CGANNNNNNTGC 2 cut(s) 547, 581
BclI TGATCA 1 cut(s) 666
BcoDI GTCTC 2 cut(s) 79, 786
BfaI CTAG 3 cut(s) 66, 272, 719
BglI GCCNNNNNGGC 2 cut(s) 59, 159
BglII AGATCT 2 cut(s) 672, 763
BisI GCNGC 3 cut(s) 51, 63, 193
BlpI GCTNAGC 1 cut(s) 750
BlsI GCNGC 3 cut(s) 52, 64, 194
BmeT110I CYCGRG 1 cut(s) 163
BmsI GCATC 2 cut(s) 33, 374
Bpu10I CCTNAGC 1 cut(s) 404
Bpu1102I GCTNAGC 1 cut(s) 750
BpuEI CTTGAG 1 cut(s) 776
Bsa29I ATCGAT 1 cut(s) 424
BsaBI GATNNNNATC 1 cut(s) 671
BsaI GGTCTC 1 cut(s) 79
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 157, 355, 375
Bse1I ACTGG 2 cut(s) 233, 588
Bse3DI GCAATG 2 cut(s) 524, 667
Bse8I GATNNNNATC 1 cut(s) 671
BseCI ATCGAT 1 cut(s) 424
BseGI GGATG 2 cut(s) 365, 481
BseJI GATNNNNATC 1 cut(s) 671
BseLI CCNNNNNNNGG 4 cut(s) 89, 157, 355, 375
BseMI GCAATG 2 cut(s) 524, 667
BseMII CTCAG 1 cut(s) 764
BseNI ACTGG 2 cut(s) 233, 588
BseSI GKGCMC 1 cut(s) 631
BseXI GCAGC 3 cut(s) 62, 74, 179
BsgI GTGCAG 1 cut(s) 619
BshVI ATCGAT 1 cut(s) 424
BsiHKCI CYCGRG 1 cut(s) 163
BslI CCNNNNNNNGG 4 cut(s) 89, 157, 355, 375
BsmAI GTCTC 2 cut(s) 79, 786
Bso31I GGTCTC 1 cut(s) 79
BsoBI CYCGRG 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 164, 631
Bsp143I GATC 4 cut(s) 421, 666, 672, 763
Bsp1720I GCTNAGC 1 cut(s) 750
BspACI CCGC 3 cut(s) 151, 485, 723
BspCNI CTCAG 1 cut(s) 763
BspDI ATCGAT 1 cut(s) 424
BspPI GGATC 1 cut(s) 429
BspTNI GGTCTC 1 cut(s) 79
BsrBI CCGCTC 1 cut(s) 487
BsrDI GCAATG 2 cut(s) 524, 667
BsrI ACTGG 2 cut(s) 233, 588
BssMI GATC 4 cut(s) 421, 666, 672, 763
Bst4CI ACNGT 1 cut(s) 790
BstC8I GCNNGC 2 cut(s) 48, 401
BstDEI CTNAG 4 cut(s) 404, 623, 750, 812
BstENI CCTNNNNNAGG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 365, 481
BstKTI GATC 4 cut(s) 424, 669, 675, 766
BstMAI GTCTC 2 cut(s) 79, 786
BstMBI GATC 4 cut(s) 421, 666, 672, 763
BstMWI GCNNNNNNNGC 2 cut(s) 59, 159
BstNSI RCATGY 3 cut(s) 16, 285, 403
BstSLI GKGCMC 1 cut(s) 631
BstV1I GCAGC 3 cut(s) 62, 74, 179
BstX2I RGATCY 2 cut(s) 672, 763
BstYI RGATCY 2 cut(s) 672, 763
Bsu15I ATCGAT 1 cut(s) 424
BsuTUI ATCGAT 1 cut(s) 424
BtsCI GGATG 2 cut(s) 365, 481
BtsIMutI CAGTG 2 cut(s) 581, 795
Cac8I GCNNGC 2 cut(s) 48, 401
ClaI ATCGAT 1 cut(s) 424
Csp6I GTAC 1 cut(s) 10
CviAII CATG 7 cut(s) 13, 282, 319, 396, 400, 490, 678
CviJI RGCY 9 cut(s) 53, 65, 162, 275, 324, 479, 511, 622, 754
CviKI_1 RGCY 9 cut(s) 53, 65, 162, 275, 324, 479, 511, 622, 754
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 4 cut(s) 404, 623, 750, 812
DpnI GATC 4 cut(s) 423, 668, 674, 765
DpnII GATC 4 cut(s) 421, 666, 672, 763
DraI TTTAAA 1 cut(s) 507
EciI GGCGGA 1 cut(s) 738
Eco24I GRGCYC 1 cut(s) 164
Eco31I GGTCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 497
Eco88I CYCGRG 1 cut(s) 163
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoT22I ATGCAT 2 cut(s) 401, 683
EcoT38I GRGCYC 1 cut(s) 164
FaeI CATG 7 cut(s) 16, 285, 322, 399, 403, 493, 681
FatI CATG 7 cut(s) 12, 281, 318, 395, 399, 489, 677
FauI CCCGC 1 cut(s) 158
FbaI TGATCA 1 cut(s) 666
Fnu4HI GCNGC 3 cut(s) 51, 63, 193
FokI GGATG 2 cut(s) 352, 468
FriOI GRGCYC 1 cut(s) 164
Fsp4HI GCNGC 3 cut(s) 51, 63, 193
FspBI CTAG 3 cut(s) 66, 272, 719
GluI GCNGC 3 cut(s) 51, 63, 193
Hin1II CATG 7 cut(s) 16, 285, 322, 399, 403, 493, 681
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 7 cut(s) 93, 141, 303, 379, 426, 552, 694
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 793
Hpy188I TCNGA 5 cut(s) 248, 298, 693, 786, 815
Hpy188III TCNNGA 5 cut(s) 23, 266, 369, 419, 670
Hpy8I GTNNAC 1 cut(s) 793
HpyAV CCTTC 2 cut(s) 464, 650
HpyCH4III ACNGT 1 cut(s) 790
HpyCH4V TGCA 7 cut(s) 50, 102, 353, 399, 529, 600, 681
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 159
HpyF3I CTNAG 4 cut(s) 404, 623, 750, 812
Hsp92II CATG 7 cut(s) 16, 285, 322, 399, 403, 493, 681
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 4 cut(s) 421, 666, 672, 763
LpnPI CCDG 6 cut(s) 88, 214, 382, 404, 569, 683
Lsp1109I GCAGC 3 cut(s) 62, 74, 179
LweI GCATC 2 cut(s) 33, 374
MaeI CTAG 3 cut(s) 66, 272, 719
MaeIII GTNAC 2 cut(s) 312, 579
MalI GATC 4 cut(s) 423, 668, 674, 765
MbiI CCGCTC 1 cut(s) 487
MboI GATC 4 cut(s) 421, 666, 672, 763
MboII GAAGA 2 cut(s) 118, 758
MfeI CAATTG 1 cut(s) 307
MflI RGATCY 2 cut(s) 672, 763
MhlI GDGCHC 2 cut(s) 164, 631
MluCI AATT 3 cut(s) 26, 307, 726
MlyI GAGTC 1 cut(s) 102
MnlI CCTC 5 cut(s) 159, 184, 349, 596, 819
Mph1103I ATGCAT 2 cut(s) 401, 683
MseI TTAA 5 cut(s) 198, 216, 374, 506, 608
MslI CAYNNNNRTG 1 cut(s) 446
MunI CAATTG 1 cut(s) 307
MwoI GCNNNNNNNGC 2 cut(s) 59, 159
NdeII GATC 4 cut(s) 421, 666, 672, 763
NlaIII CATG 7 cut(s) 16, 285, 322, 399, 403, 493, 681
NmuCI GTSAC 2 cut(s) 312, 579
NsiI ATGCAT 2 cut(s) 401, 683
NspI RCATGY 3 cut(s) 16, 285, 403
OliI CACNNNNGTG 1 cut(s) 446
PaeI GCATGC 1 cut(s) 403
PaeR7I CTCGAG 1 cut(s) 163
PciI ACATGT 2 cut(s) 12, 281
PfeI GAWTC 6 cut(s) 141, 303, 379, 426, 552, 694
PkrI GCNGC 3 cut(s) 52, 64, 194
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PscI ACATGT 2 cut(s) 12, 281
PspXI VCTCGAGB 1 cut(s) 163
PsuI RGATCY 2 cut(s) 672, 763
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 446
SaqAI TTAA 5 cut(s) 198, 216, 374, 506, 608
SatI GCNGC 3 cut(s) 51, 63, 193
Sau3AI GATC 4 cut(s) 421, 666, 672, 763
SchI GAGTC 1 cut(s) 102
SduI GDGCHC 2 cut(s) 164, 631
SfaNI GCATC 2 cut(s) 33, 374
Sfr274I CTCGAG 1 cut(s) 163
SlaI CTCGAG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 446
SmlI CTYRAG 2 cut(s) 163, 755
SmoI CTYRAG 2 cut(s) 163, 755
SphI GCATGC 1 cut(s) 403
Sse9I AATT 3 cut(s) 26, 307, 726
SsiI CCGC 3 cut(s) 151, 485, 723
SspMI CTAG 3 cut(s) 66, 272, 719
TaaI ACNGT 1 cut(s) 790
TaqI TCGA 5 cut(s) 24, 164, 258, 424, 567
TasI AATT 3 cut(s) 26, 307, 726
TfiI GAWTC 6 cut(s) 141, 303, 379, 426, 552, 694
Tru1I TTAA 5 cut(s) 198, 216, 374, 506, 608
Tru9I TTAA 5 cut(s) 198, 216, 374, 506, 608
TscAI CASTG 2 cut(s) 588, 795
TseFI GTSAC 2 cut(s) 312, 579
TseI GCWGC 3 cut(s) 50, 62, 192
Tsp45I GTSAC 2 cut(s) 312, 579
TspDTI ATGAA 1 cut(s) 384
TspRI CASTG 2 cut(s) 588, 795
XagI CCTNNNNNAGG 1 cut(s) 353
XapI RAATTY 1 cut(s) 26
XceI RCATGY 3 cut(s) 16, 285, 403
XhoI CTCGAG 1 cut(s) 163
XspI CTAG 3 cut(s) 66, 272, 719
Zsp2I ATGCAT 2 cut(s) 401, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.