Rh2AG400600

protein FAR1-RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000084
Physical Location & Seq
Forward (+)
59774159 .. 59823527
49369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG400600.1

Sequence Viewer

Length: 633 bp
ATGGTTGATAGAGGAGCTCTTCATGGTGATGGCCCGGCGCTGCTACTCGAGGAGGGAGTCCATGAAGCGGAGGTGGCCGGTGAGCTTGGGATGGATGATGCCTTTGAGGAAGCCGAAGATAAGATGGACTGGGAAATTGAAGCTTTGGATGGGGCTTTGCTGCCTACCAAAGATGGTGTTGGAATTAACCCTCAGCAAAGTGCAGGCATGGATGGATCGTATGAACCTTCAATATCTGATATTGGTTTAGTGTCCCAAAGTTCAGTAAATGGAAGTGACTGTAATGAAATGCAATATAAGGGAGTTCGGTATGATAAGTTGTCTACAGAAGATCTTAAAGGGCAGGAGTTTAAGACTGTGGAGGAAGCTGAGTCCTTTTACAATGCTTATGCAAAGGCCATGGGTTTTGATGTTAGAAGCGACTACAAGAGATTAAGTATACGCATAACAGGGAGAGTCACTTCATTGCGGTTGGTTTGTTCTGCTCAAGGGAAAAGAAGAGAAGAGTACATGAGTAACAAGAAAAGACTTCGCAAGCCAAAGAAAGAAACAAGATTCAATTGTCCATGCTTGTTCAAATCTAGTGACGTTTTTCTTTATAAAAAAGATGCCCCGAGTTTGAATTGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

210

Amino Acids

23.39

Weight (kDa)

5.08

Isoelectric Point (pI)

50.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 125 - 190 5.4e-10 FAR1 DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000084)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29034
fragaria_vesca FvH4_2g19762 FvH4_3g09170 FvH4_5g20951 FvH4_6g26050 FvH4_6g34920 FvH4_6g44020 FvH4_6g44041 FvH4_6g44050 FvH4_6g47821 FvH4_6g47821 FvH4_6g47970 FvH4_6g48151 FvH4_6g48180 FvH4_6g48300 FvH4_6g48320 FvH4_6g48330 FvH4_6g48330 FvH4_6g48330 FvH4_6g48431 FvH4_6g50821 FvH4_6g50840 FvH4_7g25361 FvH4_7g27481 FvH4_7g31230
malus_domestica MD02G1023100.v1.1 MD09G1053300.v1.1 MD09G1063900.v1.1 MD15G1165800.v1.1 MD15G1165900.v1.1
prunus_persica Prupe.3G259300_v2.0.a1 Prupe.3G259800_v2.0.a1 Prupe.3G265300_v2.0.a1 Prupe.5G213700_v2.0.a1 Prupe.7G131300_v2.0.a1 Prupe.7G249000_v2.0.a1 Prupe.7G249000_v2.0.a1 Prupe.7G249000_v2.0.a1
pyrus_communis pycom02g02000 pycom02g02010 pycom111g05350 pycom15g14860
rosa_chinensis RchiOBHm_Chr1g0366891 RchiOBHm_Chr2g0087591 RchiOBHm_Chr2g0162581 RchiOBHm_Chr2g0162591 RchiOBHm_Chr2g0166621 RchiOBHm_Chr2g0167501 RchiOBHm_Chr2g0167541 RchiOBHm_Chr2g0167551 RchiOBHm_Chr2g0167561 RchiOBHm_Chr2g0167591 RchiOBHm_Chr2g0167601 RchiOBHm_Chr2g0167641 RchiOBHm_Chr2g0167681 RchiOBHm_Chr2g0168251 RchiOBHm_Chr3g0476161 RchiOBHm_Chr3g0476181 RchiOBHm_Chr3g0476211 RchiOBHm_Chr5g0008761 RchiOBHm_Chr5g0047501 RchiOBHm_Chr6g0269121
rosa_laevigata RLG00000011477 RLG00000014026 RLG00000015309 RLG00000015881 RLG00000017161 RLG00000018247 RLG00000019905 RLG00000021634 RLG00000021635 RLG00000021680 RLG00000021681 RLG00000021725 RLG00000021734 RLG00000021735 RLG00000021739 RLG00000021741 RLG00000021745 RLG00000021756 RLG00000021758 RLG00000021759 RLG00000022096 RLG00000023339 RLG00000023786 RLG00000025547 RLG00000031523 RLG00000031601 RLG00000031628 RLG00000033737 RLG00000034477 RLG00000034478
rosa_multiflora Rmu_co8440045.1_g000001 Rmu_co8505525.1_g000001 Rmu_sc0000141.1_g000004 Rmu_sc0000148.1_g000068 Rmu_sc0000258.1_g000040 Rmu_sc0000272.1_g000035 Rmu_sc0000309.1_g000036 Rmu_sc0000335.1_g000109 Rmu_sc0000335.1_g000110 Rmu_sc0000382.1_g000008 Rmu_sc0000902.1_g000005 Rmu_sc0001392.1_g000007 Rmu_sc0001665.1_g000048 Rmu_sc0001833.1_g000013 Rmu_sc0001970.1_g000001 Rmu_sc0001970.1_g000006 Rmu_sc0001970.1_g000013 Rmu_sc0002034.1_g000008 Rmu_sc0002106.1_g000008 Rmu_sc0002268.1_g000017 Rmu_sc0002299.1_g000026 Rmu_sc0002329.1_g000037 Rmu_sc0002755.1_g000002 Rmu_sc0003264.1_g000024 Rmu_sc0003354.1_g000004 Rmu_sc0003433.1_g000010 Rmu_sc0003511.1_g000007 Rmu_sc0004888.1_g000041 Rmu_sc0006599.1_g000001 Rmu_sc0006763.1_g000016 Rmu_sc0006763.1_g000017 Rmu_sc0007003.1_g000002 Rmu_sc0007418.1_g000028 Rmu_sc0008222.1_g000002 Rmu_sc0010971.1_g000001 Rmu_sc0011091.1_g000001 Rmu_sc0011091.1_g000002 Rmu_sc0013199.1_g000005 Rmu_sc0016089.1_g000001 Rmu_sc0024549.1_g000004 Rmu_sc0024939.1_g000001 Rmu_sc0025855.1_g000003 Rmu_sc0026912.1_g000001 Rmu_sc0039772.1_g000001 Rmu_ssc0000171.1_g000028 Rmu_ssc0000171.1_g000030
rosa_roxburghii Rroxscaffold_1G00067230 Rroxscaffold_1G00075140 Rroxscaffold_2G00080050 Rroxscaffold_2G00083230 Rroxscaffold_2G00083240 Rroxscaffold_2G00083340 Rroxscaffold_2G00083390 Rroxscaffold_2G00083400 Rroxscaffold_2G00083420 Rroxscaffold_2G00083430 Rroxscaffold_2G00083940 Rroxscaffold_2G00084400 Rroxscaffold_2G00084700 Rroxscaffold_2G00084710 Rroxscaffold_2G00102510 Rroxscaffold_2G00105780 Rroxscaffold_2G00125110 Rroxscaffold_2G00125750 Rroxscaffold_2G00125790 Rroxscaffold_2G00153440 Rroxscaffold_3G00259540 Rroxscaffold_4G00314070 Rroxscaffold_5G00345290 Rroxscaffold_6G00399110 Rroxscaffold_6G00430120
rosa_rugosa Rorug01G0086600 Rorug01G0476800 Rorug02G0530700 Rorug03G0121000 Rorug03G0153000 Rorug03G0272400 Rorug03G0279800 Rorug04G0438800 Rorug05G0566700 Rorug05G0566800 Rorug06G0114400 Rorug07G0057400 Rorug07G0073700
rosa_samantha Rh1AG028900 Rh1AG062000 Rh1AG108900 Rh1AG183100 Rh1BG072000 Rh2AG030200 Rh2AG400600 Rh2AG420900 Rh2AG534000 Rh2AG590500 Rh2AG598300 Rh2AG598700 Rh2AG599000 Rh2AG599100 Rh2AG599500 Rh2AG599600 Rh2AG599900 Rh2AG600200 Rh2AG601200 Rh2AG633000 Rh2BG030200 Rh2BG097100 Rh2BG318200 Rh2BG403500 Rh2BG577200 Rh2BG602600 Rh2BG602700 Rh2BG609600 Rh2BG609700 Rh2BG610200 Rh2BG610400 Rh2BG610700 Rh2BG614300 Rh2BG646600 Rh2CG030500 Rh2CG099200 Rh2CG283200 Rh2CG386900 Rh2CG573500 Rh2CG573600 Rh2CG580400 Rh2CG580500 Rh2CG580600 Rh2CG580800 Rh2CG581100 Rh2CG582100 Rh2CG613300 Rh2CG613400 Rh2DG030400 Rh2DG613100 Rh2DG613200 Rh2DG621400 Rh2DG621600 Rh2DG621800 Rh2DG622500 Rh2DG622800 Rh2DG623100 Rh2DG623400 Rh2DG624500 Rh2DG624600 Rh2DG624800 Rh2DG661200 Rh2DG661300 Rh3AG048300 Rh3AG177800 Rh3BG203700 Rh3BG234500 Rh3BG234700 Rh3BG235200 Rh3BG330000 Rh3CG049600 Rh3CG194100 Rh3CG201200 Rh3CG230000 Rh3CG350600 Rh3DG050400 Rh3DG171400 Rh3DG304300 Rh4BG006600 Rh4BG159000 Rh4CG126300 Rh4CG171700 Rh4CG241000 Rh4DG007800 Rh4DG042200 Rh4DG311700 Rh5AG067200 Rh5AG070100 Rh5BG067000 Rh5BG247800 Rh5BG324200 Rh5BG443300 Rh5CG074800 Rh5CG078200 Rh5CG078300 Rh5CG180800 Rh5DG061800 Rh5DG066300 Rh5DG066400 Rh5DG167500 Rh5DG335000 Rh6AG174900 Rh6CG174500 Rh6CG392000 Rh6DG167000 Rh7BG185800 Rh7DG175300
rosa_wichuraiana Rw0G012810 Rw0G014220 Rw0G014230 Rw0G014240 Rw2G002410 Rw2G049760 Rw2G049770 Rw2G049780 Rw2G049800 Rw2G049820 Rw2G049920 Rw4G000180 Rw4G001290 Rw4G003630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
AccI GTMKAC 2 cut(s) 323, 439
AciI CCGC 2 cut(s) 68, 469
AclWI GGATC 1 cut(s) 223
AcoI YGGCCR 1 cut(s) 75
AfaI GTAC 1 cut(s) 509
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 5 cut(s) 140, 231, 559, 577, 622
AluBI AGCT 4 cut(s) 17, 85, 143, 368
AluI AGCT 4 cut(s) 17, 85, 143, 368
Alw21I GWGCWC 1 cut(s) 19
AlwI GGATC 1 cut(s) 223
Ama87I CYCGRG 2 cut(s) 47, 613
AoxI GGCC 3 cut(s) 31, 75, 396
ApeKI GCWGC 2 cut(s) 40, 160
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 2 cut(s) 38, 92
AvaI CYCGRG 2 cut(s) 47, 613
BanII GRGCYC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 19
BbvCI CCTCAGC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 27, 147
BccI CCATC 6 cut(s) 23, 85, 118, 143, 167, 206
BcnI CCSGG 1 cut(s) 35
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 1 cut(s) 324
BfoI RGCGCY 1 cut(s) 41
BglII AGATCT 1 cut(s) 331
BisI GCNGC 2 cut(s) 41, 161
BlsI GCNGC 2 cut(s) 42, 162
Bme1390I CCNGG 1 cut(s) 35
BmeT110I CYCGRG 2 cut(s) 47, 613
BmgT120I GGNCC 1 cut(s) 32
BmrFI CCNGG 1 cut(s) 35
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 2 cut(s) 88, 598
BmuI ACTGGG 1 cut(s) 139
Bpu10I CCTNAGC 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 471
BpuMI CCSGG 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 399
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 134
Bse3DI GCAATG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 4 cut(s) 96, 100, 154, 217
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMI GCAATG 1 cut(s) 464
BseMII CTCAG 2 cut(s) 206, 360
BseNI ACTGG 1 cut(s) 134
BseRI GAGGAG 2 cut(s) 27, 65
BseXI GCAGC 2 cut(s) 27, 147
BsgI GTGCAG 1 cut(s) 222
BshFI GGCC 3 cut(s) 33, 77, 398
BsiHKAI GWGCWC 1 cut(s) 19
BsiHKCI CYCGRG 2 cut(s) 47, 613
BsiSI CCGG 2 cut(s) 35, 78
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 1 cut(s) 67
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 3 cut(s) 33, 77, 398
BsoBI CYCGRG 2 cut(s) 47, 613
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 2 cut(s) 215, 331
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 2 cut(s) 68, 469
BspANI GGCC 3 cut(s) 33, 77, 398
BspCNI CTCAG 2 cut(s) 205, 361
BspPI GGATC 1 cut(s) 223
BspQI GCTCTTC 1 cut(s) 24
BsrDI GCAATG 1 cut(s) 464
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 1 cut(s) 134
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 2 cut(s) 215, 331
BssNAI GTATAC 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 399
Bst1107I GTATAC 1 cut(s) 440
Bst4CI ACNGT 2 cut(s) 281, 358
Bst6I CTCTTC 3 cut(s) 24, 493, 498
BstC8I GCNNGC 2 cut(s) 205, 536
BstDEI CTNAG 2 cut(s) 192, 369
BstDSI CCRYGG 1 cut(s) 399
BstF5I GGATG 4 cut(s) 96, 100, 154, 217
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 218, 334
BstMBI GATC 2 cut(s) 215, 331
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstSCI CCNGG 1 cut(s) 33
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 2 cut(s) 27, 147
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
BstZ17I GTATAC 1 cut(s) 440
BsuRI GGCC 3 cut(s) 33, 77, 398
BtgI CCRYGG 1 cut(s) 399
BtsCI GGATG 4 cut(s) 96, 100, 154, 217
Cac8I GCNNGC 2 cut(s) 205, 536
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 32
Csp6I GTAC 1 cut(s) 508
CviAII CATG 6 cut(s) 23, 62, 208, 400, 511, 567
CviQI GTAC 1 cut(s) 508
DdeI CTNAG 2 cut(s) 192, 369
DpnI GATC 2 cut(s) 217, 333
DpnII GATC 2 cut(s) 215, 331
EaeI YGGCCR 1 cut(s) 75
Eam1104I CTCTTC 3 cut(s) 24, 493, 498
EarI CTCTTC 3 cut(s) 24, 493, 498
Ecl136II GAGCTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 17
Eco88I CYCGRG 2 cut(s) 47, 613
EcoICRI GAGCTC 1 cut(s) 17
EcoT14I CCWWGG 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 6 cut(s) 26, 65, 211, 403, 514, 570
FaqI GGGAC 1 cut(s) 238
FatI CATG 6 cut(s) 22, 61, 207, 399, 510, 566
FblI GTMKAC 2 cut(s) 323, 439
Fnu4HI GCNGC 2 cut(s) 41, 161
FokI GGATG 4 cut(s) 103, 107, 161, 224
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 2 cut(s) 41, 161
FspBI CTAG 1 cut(s) 582
GlaI GCGC 1 cut(s) 39
GluI GCNGC 2 cut(s) 41, 161
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 3 cut(s) 33, 77, 398
HapII CCGG 2 cut(s) 35, 78
HhaI GCGC 1 cut(s) 40
Hin1II CATG 6 cut(s) 26, 65, 211, 403, 514, 570
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 141
HinfI GANTC 4 cut(s) 57, 371, 456, 555
HpaII CCGG 2 cut(s) 35, 78
HphI GGTGA 2 cut(s) 38, 92
Hpy166II GTNNAC 2 cut(s) 324, 440
Hpy188I TCNGA 1 cut(s) 238
Hpy8I GTNNAC 2 cut(s) 324, 440
HpyAV CCTTC 1 cut(s) 237
HpyCH4III ACNGT 2 cut(s) 281, 358
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 4 cut(s) 203, 292, 392, 627
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 2 cut(s) 192, 369
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 6 cut(s) 26, 65, 211, 403, 514, 570
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 215, 331
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 14
LpnPI CCDG 6 cut(s) 48, 91, 115, 189, 329, 435
Lsp1109I GCAGC 2 cut(s) 27, 147
LweI GCATC 2 cut(s) 88, 598
MaeI CTAG 1 cut(s) 582
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 4 cut(s) 275, 457, 515, 584
MalI GATC 2 cut(s) 217, 333
MboI GATC 2 cut(s) 215, 331
MboII GAAGA 5 cut(s) 11, 128, 341, 510, 515
MfeI CAATTG 1 cut(s) 559
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 4 cut(s) 135, 183, 559, 622
MlyI GAGTC 3 cut(s) 66, 380, 465
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 7 cut(s) 5, 43, 46, 64, 100, 201, 355
MseI TTAA 4 cut(s) 186, 336, 351, 434
MslI CAYNNNNRTG 1 cut(s) 27
MspI CCGG 2 cut(s) 35, 78
MspR9I CCNGG 1 cut(s) 35
MunI CAATTG 1 cut(s) 559
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 1 cut(s) 35
NcoI CCATGG 1 cut(s) 399
NdeII GATC 2 cut(s) 215, 331
NlaIII CATG 6 cut(s) 26, 65, 211, 403, 514, 570
NmuCI GTSAC 3 cut(s) 275, 457, 584
PaeR7I CTCGAG 1 cut(s) 47
PciSI GCTCTTC 1 cut(s) 24
PfeI GAWTC 1 cut(s) 555
PkrI GCNGC 2 cut(s) 42, 162
PleI GAGTC 3 cut(s) 65, 379, 464
PpsI GAGTC 3 cut(s) 65, 379, 464
PsiI TTATAA 1 cut(s) 600
Psp124BI GAGCTC 1 cut(s) 19
PspPI GGNCC 1 cut(s) 32
PspXI VCTCGAGB 1 cut(s) 47
PsuI RGATCY 1 cut(s) 331
RsaI GTAC 1 cut(s) 509
RsaNI GTAC 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 27
SacI GAGCTC 1 cut(s) 19
SapI GCTCTTC 1 cut(s) 24
SaqAI TTAA 4 cut(s) 186, 336, 351, 434
SatI GCNGC 2 cut(s) 41, 161
Sau3AI GATC 2 cut(s) 215, 331
Sau96I GGNCC 1 cut(s) 32
SchI GAGTC 3 cut(s) 66, 380, 465
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 19
SetI ASST 7 cut(s) 19, 75, 87, 145, 229, 370, 591
SfaNI GCATC 2 cut(s) 88, 598
SfcI CTRYAG 1 cut(s) 324
Sfr274I CTCGAG 1 cut(s) 47
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 47, 486
SmoI CTYRAG 2 cut(s) 47, 486
Sse9I AATT 4 cut(s) 135, 183, 559, 622
SsiI CCGC 2 cut(s) 68, 469
SspMI CTAG 1 cut(s) 582
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 33
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 281, 358
TaiI ACGT 1 cut(s) 591
TaqI TCGA 1 cut(s) 48
TasI AATT 4 cut(s) 135, 183, 559, 622
TatI WGTACW 1 cut(s) 507
TfiI GAWTC 1 cut(s) 555
Tru1I TTAA 4 cut(s) 186, 336, 351, 434
Tru9I TTAA 4 cut(s) 186, 336, 351, 434
TseFI GTSAC 3 cut(s) 275, 457, 584
TseI GCWGC 2 cut(s) 40, 160
Tsp45I GTSAC 3 cut(s) 275, 457, 584
TspDTI ATGAA 5 cut(s) 11, 78, 237, 300, 453
XhoI CTCGAG 1 cut(s) 47
XmiI GTMKAC 2 cut(s) 323, 439
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.