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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 423 |
| AciI | CCGC | 5 cut(s) | 54, 57, 201, 234, 282 |
| AcuI | CTGAAG | 1 cut(s) | 249 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 387 |
| AluBI | AGCT | 1 cut(s) | 323 |
| AluI | AGCT | 1 cut(s) | 323 |
| Ama87I | CYCGRG | 1 cut(s) | 18 |
| AoxI | GGCC | 2 cut(s) | 291, 430 |
| AspS9I | GGNCC | 1 cut(s) | 291 |
| AsuHPI | GGTGA | 4 cut(s) | 113, 215, 328, 421 |
| AvaI | CYCGRG | 1 cut(s) | 18 |
| BccI | CCATC | 3 cut(s) | 155, 381, 400 |
| BfaI | CTAG | 1 cut(s) | 324 |
| BisI | GCNGC | 3 cut(s) | 55, 58, 235 |
| BlsI | GCNGC | 3 cut(s) | 56, 59, 236 |
| BmeT110I | CYCGRG | 1 cut(s) | 18 |
| BmgT120I | GGNCC | 1 cut(s) | 291 |
| BmiI | GGNNCC | 2 cut(s) | 267, 292 |
| BmsI | GCATC | 1 cut(s) | 131 |
| BsaBI | GATNNNNATC | 1 cut(s) | 188 |
| BsaJI | CCNNGG | 2 cut(s) | 382, 427 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 387 |
| Bse1I | ACTGG | 1 cut(s) | 407 |
| Bse8I | GATNNNNATC | 1 cut(s) | 188 |
| BseDI | CCNNGG | 2 cut(s) | 382, 427 |
| BseGI | GGATG | 3 cut(s) | 146, 392, 411 |
| BseJI | GATNNNNATC | 1 cut(s) | 188 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 387 |
| BseNI | ACTGG | 1 cut(s) | 407 |
| BseYI | CCCAGC | 1 cut(s) | 287 |
| BshFI | GGCC | 2 cut(s) | 293, 432 |
| BsiHKCI | CYCGRG | 1 cut(s) | 18 |
| BslFI | GGGAC | 1 cut(s) | 363 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 387 |
| BsmFI | GGGAC | 1 cut(s) | 363 |
| BsnI | GGCC | 2 cut(s) | 293, 432 |
| BsoBI | CYCGRG | 1 cut(s) | 18 |
| Bsp143I | GATC | 2 cut(s) | 35, 271 |
| BspACI | CCGC | 5 cut(s) | 54, 57, 201, 234, 282 |
| BspANI | GGCC | 2 cut(s) | 293, 432 |
| BspLI | GGNNCC | 2 cut(s) | 267, 292 |
| BsrI | ACTGG | 1 cut(s) | 407 |
| BssECI | CCNNGG | 2 cut(s) | 382, 427 |
| BssMI | GATC | 2 cut(s) | 35, 271 |
| BssT1I | CCWWGG | 2 cut(s) | 382, 427 |
| Bst4CI | ACNGT | 1 cut(s) | 101 |
| Bst6I | CTCTTC | 1 cut(s) | 117 |
| BstF5I | GGATG | 3 cut(s) | 146, 392, 411 |
| BstKTI | GATC | 2 cut(s) | 38, 274 |
| BstMBI | GATC | 2 cut(s) | 35, 271 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 262, 301 |
| BsuRI | GGCC | 2 cut(s) | 293, 432 |
| BtsCI | GGATG | 3 cut(s) | 146, 392, 411 |
| Cfr13I | GGNCC | 1 cut(s) | 291 |
| CseI | GACGC | 1 cut(s) | 403 |
| CviJI | RGCY | 6 cut(s) | 219, 251, 293, 323, 374, 432 |
| CviKI_1 | RGCY | 6 cut(s) | 219, 251, 293, 323, 374, 432 |
| DpnI | GATC | 2 cut(s) | 37, 273 |
| DpnII | GATC | 2 cut(s) | 35, 271 |
| Eam1104I | CTCTTC | 1 cut(s) | 117 |
| EarI | CTCTTC | 1 cut(s) | 117 |
| Eco130I | CCWWGG | 2 cut(s) | 382, 427 |
| Eco57I | CTGAAG | 1 cut(s) | 249 |
| Eco88I | CYCGRG | 1 cut(s) | 18 |
| EcoT14I | CCWWGG | 2 cut(s) | 382, 427 |
| ErhI | CCWWGG | 2 cut(s) | 382, 427 |
| FaqI | GGGAC | 1 cut(s) | 363 |
| FblI | GTMKAC | 1 cut(s) | 423 |
| Fnu4HI | GCNGC | 3 cut(s) | 55, 58, 235 |
| FokI | GGATG | 3 cut(s) | 153, 399, 418 |
| Fsp4HI | GCNGC | 3 cut(s) | 55, 58, 235 |
| FspBI | CTAG | 1 cut(s) | 324 |
| GluI | GCNGC | 3 cut(s) | 55, 58, 235 |
| GsaI | CCCAGC | 1 cut(s) | 291 |
| HaeIII | GGCC | 2 cut(s) | 293, 432 |
| HgaI | GACGC | 1 cut(s) | 403 |
| HinfI | GANTC | 5 cut(s) | 4, 10, 121, 184, 345 |
| HphI | GGTGA | 4 cut(s) | 113, 215, 328, 421 |
| Hpy166II | GTNNAC | 2 cut(s) | 179, 424 |
| Hpy188I | TCNGA | 3 cut(s) | 9, 120, 313 |
| Hpy188III | TCNNGA | 4 cut(s) | 18, 20, 269, 275 |
| Hpy8I | GTNNAC | 2 cut(s) | 179, 424 |
| Hpy99I | CGWCG | 2 cut(s) | 101, 419 |
| HpyAV | CCTTC | 3 cut(s) | 96, 224, 335 |
| HpyCH4III | ACNGT | 1 cut(s) | 101 |
| HpyCH4V | TGCA | 2 cut(s) | 213, 359 |
| Kzo9I | GATC | 2 cut(s) | 35, 271 |
| LpnPI | CCDG | 3 cut(s) | 273, 282, 388 |
| LweI | GCATC | 1 cut(s) | 131 |
| MaeI | CTAG | 1 cut(s) | 324 |
| MaeIII | GTNAC | 1 cut(s) | 101 |
| MalI | GATC | 2 cut(s) | 37, 273 |
| MboI | GATC | 2 cut(s) | 35, 271 |
| MboII | GAAGA | 2 cut(s) | 104, 120 |
| MfeI | CAATTG | 1 cut(s) | 392 |
| MluCI | AATT | 3 cut(s) | 208, 238, 392 |
| MlyI | GAGTC | 2 cut(s) | 4, 115 |
| MmeI | TCCRAC | 3 cut(s) | 32, 149, 291 |
| MnlI | CCTC | 3 cut(s) | 131, 209, 307 |
| MunI | CAATTG | 1 cut(s) | 392 |
| NdeII | GATC | 2 cut(s) | 35, 271 |
| NlaIV | GGNNCC | 2 cut(s) | 267, 292 |
| NmuCI | GTSAC | 1 cut(s) | 101 |
| PaeR7I | CTCGAG | 1 cut(s) | 18 |
| PfeI | GAWTC | 2 cut(s) | 184, 345 |
| PkrI | GCNGC | 3 cut(s) | 56, 59, 236 |
| PleI | GAGTC | 2 cut(s) | 4, 115 |
| PpsI | GAGTC | 2 cut(s) | 4, 115 |
| PspFI | CCCAGC | 1 cut(s) | 287 |
| PspN4I | GGNNCC | 2 cut(s) | 267, 292 |
| PspPI | GGNCC | 1 cut(s) | 291 |
| SatI | GCNGC | 3 cut(s) | 55, 58, 235 |
| Sau3AI | GATC | 2 cut(s) | 35, 271 |
| Sau96I | GGNCC | 1 cut(s) | 291 |
| SchI | GAGTC | 2 cut(s) | 4, 115 |
| SetI | ASST | 4 cut(s) | 318, 325, 369, 429 |
| SfaNI | GCATC | 1 cut(s) | 131 |
| Sfr274I | CTCGAG | 1 cut(s) | 18 |
| SlaI | CTCGAG | 1 cut(s) | 18 |
| SmlI | CTYRAG | 1 cut(s) | 18 |
| SmoI | CTYRAG | 1 cut(s) | 18 |
| Sse9I | AATT | 3 cut(s) | 208, 238, 392 |
| SsiI | CCGC | 5 cut(s) | 54, 57, 201, 234, 282 |
| SspMI | CTAG | 1 cut(s) | 324 |
| StyI | CCWWGG | 2 cut(s) | 382, 427 |
| TaaI | ACNGT | 1 cut(s) | 101 |
| TaqI | TCGA | 1 cut(s) | 19 |
| TasI | AATT | 3 cut(s) | 208, 238, 392 |
| TauI | GCSGC | 3 cut(s) | 57, 60, 237 |
| TfiI | GAWTC | 2 cut(s) | 184, 345 |
| TseFI | GTSAC | 1 cut(s) | 101 |
| Tsp45I | GTSAC | 1 cut(s) | 101 |
| XhoI | CTCGAG | 1 cut(s) | 18 |
| XmiI | GTMKAC | 1 cut(s) | 423 |
| XspI | CTAG | 1 cut(s) | 324 |