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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 257 |
| AcsI | RAATTY | 1 cut(s) | 341 |
| AfaI | GTAC | 1 cut(s) | 218 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 130, 322 |
| AgsI | TTSAA | 1 cut(s) | 238 |
| AluBI | AGCT | 1 cut(s) | 231 |
| AluI | AGCT | 1 cut(s) | 231 |
| AlwI | GGATC | 1 cut(s) | 257 |
| ApoI | RAATTY | 1 cut(s) | 341 |
| AspS9I | GGNCC | 1 cut(s) | 166 |
| AvaII | GGWCC | 1 cut(s) | 166 |
| BccI | CCATC | 1 cut(s) | 81 |
| BciVI | GTATCC | 1 cut(s) | 242 |
| BclI | TGATCA | 1 cut(s) | 297 |
| BfaI | CTAG | 1 cut(s) | 317 |
| BfuI | GTATCC | 1 cut(s) | 242 |
| Bme18I | GGWCC | 1 cut(s) | 166 |
| BmgT120I | GGNCC | 1 cut(s) | 166 |
| BmiI | GGNNCC | 1 cut(s) | 167 |
| BmsI | GCATC | 1 cut(s) | 124 |
| BpmI | CTGGAG | 1 cut(s) | 90 |
| BsaBI | GATNNNNATC | 1 cut(s) | 261 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 136, 166 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 130, 322 |
| Bse118I | RCCGGY | 1 cut(s) | 42 |
| Bse1I | ACTGG | 1 cut(s) | 124 |
| Bse3DI | GCAATG | 2 cut(s) | 171, 298 |
| Bse8I | GATNNNNATC | 1 cut(s) | 261 |
| BseGI | GGATG | 1 cut(s) | 139 |
| BseJI | GATNNNNATC | 1 cut(s) | 261 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 130, 322 |
| BseMI | GCAATG | 2 cut(s) | 171, 298 |
| BseNI | ACTGG | 1 cut(s) | 124 |
| BsiSI | CCGG | 1 cut(s) | 43 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 130, 322 |
| BsmI | GAATGC | 1 cut(s) | 31 |
| Bsp1407I | TGTACA | 1 cut(s) | 216 |
| Bsp143I | GATC | 3 cut(s) | 256, 262, 297 |
| BspHI | TCATGA | 2 cut(s) | 208, 259 |
| BspLI | GGNNCC | 1 cut(s) | 167 |
| BspPI | GGATC | 1 cut(s) | 257 |
| BsrDI | GCAATG | 2 cut(s) | 171, 298 |
| BsrFI | RCCGGY | 1 cut(s) | 42 |
| BsrGI | TGTACA | 1 cut(s) | 216 |
| BsrI | ACTGG | 1 cut(s) | 124 |
| BssAI | RCCGGY | 1 cut(s) | 42 |
| BssMI | GATC | 3 cut(s) | 256, 262, 297 |
| Bst4CI | ACNGT | 2 cut(s) | 180, 227 |
| BstAUI | TGTACA | 1 cut(s) | 216 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 320 |
| BstF5I | GGATG | 1 cut(s) | 139 |
| BstKTI | GATC | 3 cut(s) | 259, 265, 300 |
| BstMBI | GATC | 3 cut(s) | 256, 262, 297 |
| BsuI | GTATCC | 1 cut(s) | 242 |
| BtsCI | GGATG | 1 cut(s) | 139 |
| BtsIMutI | CAGTG | 1 cut(s) | 131 |
| CciI | TCATGA | 2 cut(s) | 208, 259 |
| Cfr10I | RCCGGY | 1 cut(s) | 42 |
| Cfr13I | GGNCC | 1 cut(s) | 166 |
| Csp6I | GTAC | 1 cut(s) | 217 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 50, 85 |
| CviAII | CATG | 2 cut(s) | 209, 260 |
| CviJI | RGCY | 1 cut(s) | 231 |
| CviKI_1 | RGCY | 1 cut(s) | 231 |
| CviQI | GTAC | 1 cut(s) | 217 |
| DpnI | GATC | 3 cut(s) | 258, 264, 299 |
| DpnII | GATC | 3 cut(s) | 256, 262, 297 |
| Eco47I | GGWCC | 1 cut(s) | 166 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 320 |
| FaeI | CATG | 2 cut(s) | 212, 263 |
| FaiI | YATR | 9 cut(s) | 34, 40, 141, 200, 210, 261, 269, 288, 378 |
| FatI | CATG | 2 cut(s) | 208, 259 |
| FbaI | TGATCA | 1 cut(s) | 297 |
| FokI | GGATG | 1 cut(s) | 146 |
| FspBI | CTAG | 1 cut(s) | 317 |
| GsuI | CTGGAG | 1 cut(s) | 90 |
| HapII | CCGG | 1 cut(s) | 43 |
| Hin1II | CATG | 2 cut(s) | 212, 263 |
| HinfI | GANTC | 1 cut(s) | 10 |
| HpaII | CCGG | 1 cut(s) | 43 |
| Hpy166II | GTNNAC | 2 cut(s) | 169, 217 |
| Hpy188III | TCNNGA | 4 cut(s) | 107, 209, 238, 260 |
| Hpy8I | GTNNAC | 2 cut(s) | 169, 217 |
| HpyCH4III | ACNGT | 2 cut(s) | 180, 227 |
| HpyCH4V | TGCA | 2 cut(s) | 176, 188 |
| Hsp92II | CATG | 2 cut(s) | 212, 263 |
| Ksp22I | TGATCA | 1 cut(s) | 297 |
| Kzo9I | GATC | 3 cut(s) | 256, 262, 297 |
| LpnPI | CCDG | 6 cut(s) | 27, 56, 116, 120, 137, 231 |
| LweI | GCATC | 1 cut(s) | 124 |
| MaeI | CTAG | 1 cut(s) | 317 |
| MalI | GATC | 3 cut(s) | 258, 264, 299 |
| MboI | GATC | 3 cut(s) | 256, 262, 297 |
| MfeI | CAATTG | 3 cut(s) | 67, 183, 367 |
| MluCI | AATT | 6 cut(s) | 67, 183, 341, 349, 367, 388 |
| MnlI | CCTC | 1 cut(s) | 156 |
| MseI | TTAA | 1 cut(s) | 278 |
| MspI | CCGG | 1 cut(s) | 43 |
| MunI | CAATTG | 3 cut(s) | 67, 183, 367 |
| Mva1269I | GAATGC | 1 cut(s) | 31 |
| NdeII | GATC | 3 cut(s) | 256, 262, 297 |
| NlaIII | CATG | 2 cut(s) | 212, 263 |
| NlaIV | GGNNCC | 1 cut(s) | 167 |
| PagI | TCATGA | 2 cut(s) | 208, 259 |
| PctI | GAATGC | 1 cut(s) | 31 |
| PfeI | GAWTC | 1 cut(s) | 10 |
| PspN4I | GGNNCC | 1 cut(s) | 167 |
| PspPI | GGNCC | 1 cut(s) | 166 |
| RsaI | GTAC | 1 cut(s) | 218 |
| RsaNI | GTAC | 1 cut(s) | 217 |
| SaqAI | TTAA | 1 cut(s) | 278 |
| Sau3AI | GATC | 3 cut(s) | 256, 262, 297 |
| Sau96I | GGNCC | 1 cut(s) | 166 |
| SetI | ASST | 3 cut(s) | 233, 318, 327 |
| SfaNI | GCATC | 1 cut(s) | 124 |
| SinI | GGWCC | 1 cut(s) | 166 |
| Sse9I | AATT | 6 cut(s) | 67, 183, 341, 349, 367, 388 |
| SspMI | CTAG | 1 cut(s) | 317 |
| TaaI | ACNGT | 2 cut(s) | 180, 227 |
| TaqI | TCGA | 1 cut(s) | 356 |
| TasI | AATT | 6 cut(s) | 67, 183, 341, 349, 367, 388 |
| TatI | WGTACW | 1 cut(s) | 216 |
| TfiI | GAWTC | 1 cut(s) | 10 |
| Tru1I | TTAA | 1 cut(s) | 278 |
| Tru9I | TTAA | 1 cut(s) | 278 |
| TscAI | CASTG | 1 cut(s) | 131 |
| TspDTI | ATGAA | 2 cut(s) | 197, 225 |
| TspRI | CASTG | 1 cut(s) | 131 |
| VpaK11BI | GGWCC | 1 cut(s) | 166 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 320 |
| XapI | RAATTY | 1 cut(s) | 341 |
| XspI | CTAG | 1 cut(s) | 317 |