Rh2DG357300

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000442
Physical Location & Seq
Forward (+)
50306839 .. 50310287
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG357300.1

Sequence Viewer

Length: 246 bp
ATGGTTGAAGCATATCCAATGAAAGGTGGAGATGGACCCAACAGCTATGCCAAGAACTCCAGTTACCAGATTAGTTGCAGAAGACGCAGCAGCCATTGCTTGCCGGCTCTAGCTATGTTCTCTGATCTCGATTCAGGTAGATATAATGTAGATCCAACTTTCTATTCACTTGTGGGATCCGGACAGTCCATGAAGGCACCATGGCTCCTCTCTTCTCAAGAATCCATACATCCCTCATCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

81

Amino Acids

8.85

Weight (kDa)

7.8

Isoelectric Point (pI)

77.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2647 PF10839 47 - 81 5.3e-07 Protein of unknown function (DUF2647)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000442)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g43021 FvH4_6g46860 FvH4_6g46860 FvH4_6g46860 FvH4_6g46860 FvH4_6g46882 FvH4_6g51201
rosa_chinensis RchiOBHm_Chr2g0171801 RchiOBHm_Chr6g0301581 RchiOBHm_Chr7g0179011
rosa_laevigata RLG00000005529 RLG00000009488 RLG00000017141 RLG00000019517 RLG00000022054 RLG00000022057 RLG00000022071 RLG00000022073 RLG00000022076 RLG00000022284 RLG00000023212 RLG00000023370 RLG00000024583 RLG00000027622 RLG00000033172
rosa_multiflora Rmu_sc0000888.1_g000012 Rmu_sc0002026.1_g000021
rosa_roxburghii Rroxscaffold_1G00033790 Rroxscaffold_2G00080150 Rroxscaffold_2G00080220 Rroxscaffold_2G00080240 Rroxscaffold_2G00080330 Rroxscaffold_2G00080440 Rroxscaffold_2G00080470 Rroxscaffold_4G00323360 Rroxscaffold_5G00345900
rosa_rugosa Rorug02G0085600 Rorug02G0555900 Rorug02G0556000 Rorug02G0556200 Rorug02G0556300
rosa_samantha Rh1AG323200 Rh1BG273400 Rh2AG630700 Rh2AG630900 Rh2AG631900 Rh2AG636400 Rh2BG636800 Rh2BG641800 Rh2BG641900 Rh2BG642000 Rh2BG643800 Rh2BG644200 Rh2BG644700 Rh2BG645400 Rh2BG649800 Rh2CG610100 Rh2CG610200 Rh2CG610900 Rh2CG611000 Rh2CG611100 Rh2CG611800 Rh2CG612100 Rh2CG612300 Rh2CG612400 Rh2DG357300 Rh2DG652600 Rh2DG652800 Rh2DG652900 Rh2DG653100 Rh2DG658100 Rh2DG659200 Rh2DG659300 Rh2DG659800 Rh2DG660100 Rh2DG660300 Rh2DG660400 Rh3AG273000 Rh3DG210900 Rh4AG004400 Rh4AG104800 Rh4AG105200 Rh4AG433400 Rh5AG430100 Rh5BG445500 Rh6AG494800 Rh6DG386400 Rh7AG193900 Rh7CG200400
rosa_wichuraiana Rw2G052220 Rw2G052280 Rw2G052290 Rw2G052320 Rw2G052330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AccIII TCCGGA 1 cut(s) 179
AclWI GGATC 3 cut(s) 146, 171, 184
AfiI CCNNNNNNNGG 1 cut(s) 23
AgsI TTSAA 1 cut(s) 8
AjuI GAANNNNNNNTTGG 2 cut(s) 148, 180
AluBI AGCT 2 cut(s) 45, 113
AluI AGCT 2 cut(s) 45, 113
AlwI GGATC 3 cut(s) 146, 171, 184
Aor13HI TCCGGA 1 cut(s) 179
ApeKI GCWGC 2 cut(s) 87, 90
AspS9I GGNCC 1 cut(s) 35
AvaII GGWCC 1 cut(s) 35
BamHI GGATCC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 196
BbsI GAAGAC 1 cut(s) 88
BbvI GCAGC 2 cut(s) 99, 102
BccI CCATC 1 cut(s) 26
BfaI CTAG 1 cut(s) 110
BisI GCNGC 2 cut(s) 88, 91
BlsI GCNGC 2 cut(s) 89, 92
Bme18I GGWCC 1 cut(s) 35
BmgT120I GGNCC 1 cut(s) 35
BmiI GGNNCC 4 cut(s) 37, 178, 198, 206
BpiI GAAGAC 1 cut(s) 88
BpmI CTGGAG 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 200
BsaWI WCCGGW 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 1 cut(s) 23
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 1 cut(s) 60
Bse3DI GCAATG 1 cut(s) 94
BseAI TCCGGA 1 cut(s) 179
BseDI CCNNGG 1 cut(s) 200
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 1 cut(s) 23
BseMI GCAATG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 60
BseRI GAGGAG 1 cut(s) 197
BseXI GCAGC 2 cut(s) 99, 102
BshNI GGYRCC 1 cut(s) 196
BsiSI CCGG 2 cut(s) 104, 180
BslI CCNNNNNNNGG 1 cut(s) 23
Bsp13I TCCGGA 1 cut(s) 179
Bsp143I GATC 3 cut(s) 124, 151, 176
Bsp19I CCATGG 1 cut(s) 200
BspEI TCCGGA 1 cut(s) 179
BspLI GGNNCC 4 cut(s) 37, 178, 198, 206
BspPI GGATC 3 cut(s) 146, 171, 184
BspT107I GGYRCC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 1 cut(s) 60
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 200
BssMI GATC 3 cut(s) 124, 151, 176
BssT1I CCWWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 186
Bst6I CTCTTC 1 cut(s) 217
BstAPI GCANNNNNTGC 1 cut(s) 96
BstC8I GCNNGC 2 cut(s) 101, 105
BstDSI CCRYGG 1 cut(s) 200
BstF5I GGATG 1 cut(s) 229
BstKTI GATC 3 cut(s) 127, 154, 179
BstMBI GATC 3 cut(s) 124, 151, 176
BstMWI GCNNNNNNNGC 2 cut(s) 84, 96
BstV1I GCAGC 2 cut(s) 99, 102
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 151, 176
BstYI RGATCY 2 cut(s) 151, 176
BtgI CCRYGG 1 cut(s) 200
BtsCI GGATG 1 cut(s) 229
Cac8I GCNNGC 2 cut(s) 101, 105
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 1 cut(s) 35
CseI GACGC 1 cut(s) 93
CviAII CATG 2 cut(s) 190, 201
CviJI RGCY 5 cut(s) 45, 93, 107, 113, 205
CviKI_1 RGCY 5 cut(s) 45, 93, 107, 113, 205
DpnI GATC 3 cut(s) 126, 153, 178
DpnII GATC 3 cut(s) 124, 151, 176
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 35
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
FaeI CATG 2 cut(s) 193, 204
FaiI YATR 8 cut(s) 13, 48, 116, 144, 191, 202, 227, 244
FatI CATG 2 cut(s) 189, 200
Fnu4HI GCNGC 2 cut(s) 88, 91
FokI GGATG 1 cut(s) 216
Fsp4HI GCNGC 2 cut(s) 88, 91
FspBI CTAG 1 cut(s) 110
GluI GCNGC 2 cut(s) 88, 91
GsuI CTGGAG 1 cut(s) 43
HapII CCGG 2 cut(s) 104, 180
HgaI GACGC 1 cut(s) 93
Hin1II CATG 2 cut(s) 193, 204
HinfI GANTC 2 cut(s) 131, 221
HpaII CCGG 2 cut(s) 104, 180
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 3 cut(s) 128, 180, 218
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 1 cut(s) 186
HpyCH4V TGCA 1 cut(s) 78
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 96
Hsp92II CATG 2 cut(s) 193, 204
Kpn2I TCCGGA 1 cut(s) 179
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Kzo9I GATC 3 cut(s) 124, 151, 176
LmnI GCTCC 1 cut(s) 210
LpnPI CCDG 5 cut(s) 73, 80, 117, 120, 193
Lsp1109I GCAGC 2 cut(s) 99, 102
MaeI CTAG 1 cut(s) 110
MaeIII GTNAC 1 cut(s) 62
MalI GATC 3 cut(s) 126, 153, 178
MboI GATC 3 cut(s) 124, 151, 176
MboII GAAGA 2 cut(s) 93, 204
MflI RGATCY 2 cut(s) 151, 176
MmeI TCCRAC 1 cut(s) 179
MnlI CCTC 2 cut(s) 218, 244
MroI TCCGGA 1 cut(s) 179
MroNI GCCGGC 1 cut(s) 103
MspI CCGG 2 cut(s) 104, 180
MwoI GCNNNNNNNGC 2 cut(s) 84, 96
NaeI GCCGGC 1 cut(s) 105
NcoI CCATGG 1 cut(s) 200
NdeII GATC 3 cut(s) 124, 151, 176
NgoMIV GCCGGC 1 cut(s) 103
NlaIII CATG 2 cut(s) 193, 204
NlaIV GGNNCC 4 cut(s) 37, 178, 198, 206
PdiI GCCGGC 1 cut(s) 105
PfeI GAWTC 2 cut(s) 131, 221
PkrI GCNGC 2 cut(s) 89, 92
PspN4I GGNNCC 4 cut(s) 37, 178, 198, 206
PspPI GGNCC 1 cut(s) 35
PsrI GAACNNNNNNTAC 2 cut(s) 47, 79
PsuI RGATCY 2 cut(s) 151, 176
SatI GCNGC 2 cut(s) 88, 91
Sau3AI GATC 3 cut(s) 124, 151, 176
Sau96I GGNCC 1 cut(s) 35
SetI ASST 4 cut(s) 28, 47, 115, 139
SinI GGWCC 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
SspMI CTAG 1 cut(s) 110
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 186
TaqI TCGA 1 cut(s) 129
TfiI GAWTC 2 cut(s) 131, 221
TseI GCWGC 2 cut(s) 87, 90
TspDTI ATGAA 2 cut(s) 35, 206
VpaK11BI GGWCC 1 cut(s) 35
XspI CTAG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.