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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 147 |
| AarI | CACCTGC | 1 cut(s) | 74 |
| Acc36I | ACCTGC | 1 cut(s) | 74 |
| AccI | GTMKAC | 1 cut(s) | 63 |
| AclWI | GGATC | 1 cut(s) | 152 |
| AfaI | GTAC | 2 cut(s) | 22, 113 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 54 |
| AgsI | TTSAA | 4 cut(s) | 78, 133, 331, 344 |
| AluBI | AGCT | 3 cut(s) | 126, 143, 226 |
| AluI | AGCT | 3 cut(s) | 126, 143, 226 |
| AlwI | GGATC | 1 cut(s) | 152 |
| AsuHPI | GGTGA | 1 cut(s) | 28 |
| AsuNHI | GCTAGC | 1 cut(s) | 89 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 169, 202 |
| BccI | CCATC | 2 cut(s) | 52, 56 |
| BclI | TGATCA | 1 cut(s) | 192 |
| BfaI | CTAG | 1 cut(s) | 90 |
| BfuAI | ACCTGC | 1 cut(s) | 74 |
| BmsI | GCATC | 1 cut(s) | 250 |
| BmtI | GCTAGC | 1 cut(s) | 93 |
| BpmI | CTGGAG | 2 cut(s) | 285, 302 |
| BsaBI | GATNNNNATC | 1 cut(s) | 156 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 54 |
| Bse1I | ACTGG | 1 cut(s) | 22 |
| Bse3DI | GCAATG | 1 cut(s) | 66 |
| Bse8I | GATNNNNATC | 1 cut(s) | 156 |
| BseGI | GGATG | 2 cut(s) | 63, 366 |
| BseJI | GATNNNNATC | 1 cut(s) | 156 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 54 |
| BseMI | GCAATG | 1 cut(s) | 66 |
| BseNI | ACTGG | 1 cut(s) | 22 |
| BslFI | GGGAC | 1 cut(s) | 316 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 54 |
| BsmFI | GGGAC | 1 cut(s) | 316 |
| Bsp1407I | TGTACA | 1 cut(s) | 111 |
| Bsp143I | GATC | 3 cut(s) | 151, 157, 192 |
| BspMI | ACCTGC | 1 cut(s) | 74 |
| BspOI | GCTAGC | 1 cut(s) | 93 |
| BspPI | GGATC | 1 cut(s) | 152 |
| BsrDI | GCAATG | 1 cut(s) | 66 |
| BsrGI | TGTACA | 1 cut(s) | 111 |
| BsrI | ACTGG | 1 cut(s) | 22 |
| BssMI | GATC | 3 cut(s) | 151, 157, 192 |
| Bst4CI | ACNGT | 1 cut(s) | 185 |
| BstAUI | TGTACA | 1 cut(s) | 111 |
| BstC8I | GCNNGC | 1 cut(s) | 91 |
| BstDEI | CTNAG | 1 cut(s) | 227 |
| BstF5I | GGATG | 2 cut(s) | 63, 366 |
| BstKTI | GATC | 3 cut(s) | 154, 160, 195 |
| BstMBI | GATC | 3 cut(s) | 151, 157, 192 |
| BtgZI | GCGATG | 1 cut(s) | 227 |
| BtsCI | GGATG | 2 cut(s) | 63, 366 |
| BveI | ACCTGC | 1 cut(s) | 74 |
| Cac8I | GCNNGC | 1 cut(s) | 91 |
| Csp6I | GTAC | 2 cut(s) | 21, 112 |
| CviAII | CATG | 1 cut(s) | 104 |
| CviJI | RGCY | 4 cut(s) | 126, 143, 226, 263 |
| CviKI_1 | RGCY | 4 cut(s) | 126, 143, 226, 263 |
| CviQI | GTAC | 2 cut(s) | 21, 112 |
| DdeI | CTNAG | 1 cut(s) | 227 |
| DpnI | GATC | 3 cut(s) | 153, 159, 194 |
| DpnII | GATC | 3 cut(s) | 151, 157, 192 |
| Eco32I | GATATC | 1 cut(s) | 289 |
| EcoRV | GATATC | 1 cut(s) | 289 |
| FaeI | CATG | 1 cut(s) | 107 |
| FaiI | YATR | 7 cut(s) | 15, 105, 147, 164, 207, 213, 355 |
| FaqI | GGGAC | 1 cut(s) | 316 |
| FatI | CATG | 1 cut(s) | 103 |
| FbaI | TGATCA | 1 cut(s) | 192 |
| FblI | GTMKAC | 1 cut(s) | 63 |
| FokI | GGATG | 2 cut(s) | 70, 373 |
| FspBI | CTAG | 1 cut(s) | 90 |
| GsuI | CTGGAG | 2 cut(s) | 285, 302 |
| Hin1II | CATG | 1 cut(s) | 107 |
| HindIII | AAGCTT | 1 cut(s) | 141 |
| HphI | GGTGA | 1 cut(s) | 28 |
| Hpy166II | GTNNAC | 2 cut(s) | 64, 112 |
| Hpy188III | TCNNGA | 2 cut(s) | 155, 281 |
| Hpy8I | GTNNAC | 2 cut(s) | 64, 112 |
| HpyAV | CCTTC | 1 cut(s) | 350 |
| HpyCH4III | ACNGT | 1 cut(s) | 185 |
| HpyCH4IV | ACGT | 1 cut(s) | 73 |
| HpyCH4V | TGCA | 3 cut(s) | 71, 83, 215 |
| HpyF3I | CTNAG | 1 cut(s) | 227 |
| HpySE526I | ACGT | 1 cut(s) | 73 |
| Hsp92II | CATG | 1 cut(s) | 107 |
| Ksp22I | TGATCA | 1 cut(s) | 192 |
| Kzo9I | GATC | 3 cut(s) | 151, 157, 192 |
| LmnI | GCTCC | 1 cut(s) | 310 |
| LpnPI | CCDG | 4 cut(s) | 3, 69, 249, 266 |
| LweI | GCATC | 1 cut(s) | 250 |
| MaeI | CTAG | 1 cut(s) | 90 |
| MaeII | ACGT | 1 cut(s) | 73 |
| MalI | GATC | 3 cut(s) | 153, 159, 194 |
| MboI | GATC | 3 cut(s) | 151, 157, 192 |
| MboII | GAAGA | 1 cut(s) | 328 |
| MfeI | CAATTG | 2 cut(s) | 42, 78 |
| MluCI | AATT | 2 cut(s) | 42, 78 |
| MnlI | CCTC | 4 cut(s) | 277, 292, 351, 357 |
| MseI | TTAA | 2 cut(s) | 173, 376 |
| MslI | CAYNNNNRTG | 1 cut(s) | 204 |
| MunI | CAATTG | 2 cut(s) | 42, 78 |
| NdeII | GATC | 3 cut(s) | 151, 157, 192 |
| NheI | GCTAGC | 1 cut(s) | 89 |
| NlaIII | CATG | 1 cut(s) | 107 |
| PaqCI | CACCTGC | 1 cut(s) | 74 |
| PsiI | TTATAA | 1 cut(s) | 147 |
| RsaI | GTAC | 2 cut(s) | 22, 113 |
| RsaNI | GTAC | 2 cut(s) | 21, 112 |
| RseI | CAYNNNNRTG | 1 cut(s) | 204 |
| SaqAI | TTAA | 2 cut(s) | 173, 376 |
| Sau3AI | GATC | 3 cut(s) | 151, 157, 192 |
| SetI | ASST | 6 cut(s) | 76, 88, 128, 145, 204, 228 |
| SfaNI | GCATC | 1 cut(s) | 250 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 204 |
| Sse9I | AATT | 2 cut(s) | 42, 78 |
| SspMI | CTAG | 1 cut(s) | 90 |
| TaaI | ACNGT | 1 cut(s) | 185 |
| TaiI | ACGT | 1 cut(s) | 76 |
| TasI | AATT | 2 cut(s) | 42, 78 |
| TatI | WGTACW | 1 cut(s) | 111 |
| Tru1I | TTAA | 2 cut(s) | 173, 376 |
| Tru9I | TTAA | 2 cut(s) | 173, 376 |
| TspDTI | ATGAA | 2 cut(s) | 92, 222 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 55 |
| XmiI | GTMKAC | 1 cut(s) | 63 |
| XspI | CTAG | 1 cut(s) | 90 |