Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000257

Physical Location & Seq

Genomic Coordinates Forward (+)

17251011 .. 17254113

Gene Structure 3103 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G46840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2217 bp

ATGACAAAACCTGCTGTCTCCTATTGTCTCCTCTCATGTATCTTTGCCTTGCTTGTTGTGAGTTTCGCTTCAGCAGACAAAGATGATCAAGACAAACAGGAGTATATAGTATACATGGGTGCACTTCCTGCTCGTGTTGACTACATGCCTATGTCCCATCACACTAGTATTCTTCAAGATGTCACCGGAGAGAGTTCGATCGAGGATCGTTTGGTTAGGAATTATAAAAGGAGTTTCAACGGATTCGCAGCCCGGCTAACCAAATCCGAAAGAGAGATACTAGCAAGTATGGACGAAGTTGTGTCTGTGTTTCCTAACAAGAAACTGAAGCTCCAAACAACAACGTCATGGAATTTCATGGGCCTCAAGGAAAGCAAACGTACAAAGCGAAATACTATCATAGAGAGTGATACAATCATCGGTGTCATCGACAGTGGAATCTATCCGGAATCTGATAGTTTTAGTGGCAAAGGCTTTGGTCCTCCTCCTAAAAAATGGAAAGGTGTTTGCAAAGGCGGCAAAAACTTCACTTGGAACAACAAGCTTATTGGAGCACGGTACTACACCCCCAAACTAGAGGGGTTTCCTGAGTCAGCGAGGGACTACATGGGACATGGATCGCACACAGCATCCACGGCTGCTGGAAATGCTGTGAAGCATGTGAGCTTTTACGGCCTCGGCAATGGAACCGCAAGAGGTGGCGTTCCAGCTGCAAGAATAGCGGTTTATAAAGTATGTGACCCTGGCGTCGATGGGTGCACAACTGACGGAATTCTAGCGGCATTTGATGATGCCATAGCGGATAAGGTGGACATCATTACCATATCCATTGGTGGTGATAATAGTTCGCCTTTTGAGGAGGACCCTATCGCGATCGGGGCTTTCCATGCCATGGCCAAGGGAATCCTCATAGTGAATTCCGCAGGAAACAGTGGTCCAGAACCTAGCACGGTCGCGAGTATTGCGCCGTGGATGTTCACTGTTGCTGCTAGTAATACTAACCGTGCGTTTGTCACTAAAGTTGTTCTCGGAAACGGCAAGACAGTTGGAAGATCGGTAAATTCGTTCGATCTTAACGGAAAGAAGTACCCTCTAGTATACGGGAAATCAGCTTCAAGTAGTTGTGGTGCTGCTTCCGCCGGGTTTTGCTCACCAGGGTGCCTAGATAGTAAACGTGTGAAAGGAAAGATTGTTTTATGTGATTCCCCGCAAAATCCCGATGAAGCTCAAGCTATGGGAGCCATTGCATCTATTGTTAGGAGCCACCGTACAGACGTGGCAAGTATCTTCTCTTTTCCAGTCTCTGTTCTATTGGAAGACGACTATAACACTGTTCTCTCGTATATGAACTCCACCAAGAATCCCAAAGCGGCTGTTCTGAAAAGTGAGACAATCTTTAACCAGAGAGCTCCCGTCGTGGCTTCCTACTTTTCTCGTGGCCCAAACACAATCATTCCTGACATATTGAAGCCGGATATAACGGCACCAGGATCAGAGATCGTAGCTGCTTATTCTCCTGATGCCCCACCAAGCATATCAGATACAAGACGTGTAAAGTACTCCGTTGATACCGGAACTTCAATGTCTTGTCCGCATGTTGCTGGTGTAGCTGCCTACTTAAAATCGTTTCACCCACGCTGGTCTCCCTCCATGATCCAATCAGCCATCATGACTACCGCTTGGCCAATGAATGCATCTACTTCTCCATTTAACGAGTTGGCCGAGTTTGCTTACGGAGCTGGCCATGTCGATCCCATAACTGCAATTCATCCTGGACTAGTCTATGAAGCTAATAAATCCGACCACATCGCCTTTCTCTGCGGCTTGAACTACACTGCAAAGAACTTAAGACTCATCTCCGGTGATAGCAGCAGTTGCACAAAAGAACAGACTAAATCGCTACCCCGAAACTTAAACTATCCTTCGATGACTGCTCAAGTATCGGCGGCAAAACCATTCAAAGTGATTTTCCGTAGAACAGTCACTAACGTCGGTAGGCCTAACGCTACATACAAAGCAAAGGTAGTCGGTTCTAAACTCAAAGTCAAGGTTGTTCCGGCAGTCTTGTCTTTAAAGTCATTGTACGAGAAGAAGTCCTTCACGGTAACAGCTTCCGGCGCCGGTCCCAAAGCTGAAAATCTTGTCTCCGCACAGCTGATCTGGTCTGATGGTGTCCACTTTGTGAGAAGCCCCATTGTTGTTTATGCTACGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0010035 GO:0010038 GO:0016787 GO:0017171 GO:0019538 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044464 GO:0046686 GO:0050896 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

738

Amino Acids

79.06

Weight (kDa)

9.2

Isoelectric Point (pI)

38.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	35 - 112	2.1e-18	Peptidase inhibitor I9
Peptidase_S8	PF00082	136 - 581	6.6e-41	Subtilase family
fn3_6	PF17766	637 - 734	1.1e-25	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca	FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica	MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica	Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis	pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis	RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata	RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora	Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii	Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa	Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha	Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana	Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	225, 729
AasI	GACNNNNNNGTC	1 cut(s)	746
Acc36I	ACCTGC	1 cut(s)	19
AccB1I	GGYRCC	3 cut(s)	1158, 1483, 2117
AccB7I	CCANNNNNTGG	1 cut(s)	892
AccI	GTMKAC	2 cut(s)	111, 1098
AccII	CGCG	2 cut(s)	872, 956
AccIII	TCCGGA	1 cut(s)	445
AclWI	GGATC	5 cut(s)	213, 625, 1498, 1648, 1745
AcoI	YGGCCR	4 cut(s)	894, 1682, 1719, 1741
AcsI	RAATTY	4 cut(s)	352, 771, 916, 1060
AcuI	CTGAAG	2 cut(s)	54, 347
AcyI	GRCGYC	2 cut(s)	747, 2118
AdeI	CACNNNGTG	1 cut(s)	2182
AfaI	GTAC	6 cut(s)	382, 560, 1088, 1270, 1559, 2084
AfiI	CCNNNNNNNGG	1 cut(s)	892
AflII	CTTAAG	1 cut(s)	1846
AflIII	ACRYGT	2 cut(s)	1174, 1549
AgsI	TTSAA	7 cut(s)	176, 238, 1116, 1468, 1581, 1828, 1960
AhlI	ACTAGT	2 cut(s)	164, 1777
AjiI	CACGTC	2 cut(s)	1276, 1550
AjnI	CCWGG	4 cut(s)	742, 1153, 1486, 1771
AleI	CACNNNNGTG	1 cut(s)	1156
Alw21I	GWGCWC	4 cut(s)	124, 556, 761, 1411
Alw26I	GTCTC	6 cut(s)	22, 32, 1306, 1382, 1647, 2149
Alw44I	GTGCAC	2 cut(s)	120, 757
AlwI	GGATC	5 cut(s)	213, 625, 1498, 1648, 1745
AlwNI	CAGNNNCTG	1 cut(s)	1304
Aor13HI	TCCGGA	1 cut(s)	445
AoxI	GGCC	8 cut(s)	361, 673, 894, 1438, 1682, 1719, 1741, 1997
ApaLI	GTGCAC	2 cut(s)	120, 757
ApeKI	GCWGC	8 cut(s)	248, 638, 710, 986, 1130, 1505, 1610, 1869
ApoI	RAATTY	4 cut(s)	352, 771, 916, 1060
Asp700I	GAANNNNTTC	1 cut(s)	2096
AspLEI	GCGC	2 cut(s)	967, 2120
AspS9I	GGNCC	6 cut(s)	361, 479, 862, 935, 1439, 2123
AsuC2I	CCSGG	2 cut(s)	253, 1141
AsuHPI	GGTGA	5 cut(s)	175, 848, 1143, 1622, 1874
AvaII	GGWCC	4 cut(s)	479, 862, 935, 2123
BaeGI	GKGCMC	2 cut(s)	124, 761
BalI	TGGCCA	3 cut(s)	896, 1684, 1743
BanI	GGYRCC	3 cut(s)	1158, 1483, 2117
BanII	GRGCYC	1 cut(s)	1411
BauI	CACGAG	2 cut(s)	132, 1434
BbsI	GAAGAC	1 cut(s)	1323
Bbv12I	GWGCWC	4 cut(s)	124, 556, 761, 1411
BbvI	GCAGC	8 cut(s)	260, 625, 697, 973, 1117, 1492, 1597, 1881
BccI	CCATC	4 cut(s)	165, 746, 1673, 2162
BceAI	ACGGC	5 cut(s)	651, 688, 952, 1051, 1497
BciT130I	CCWGG	4 cut(s)	744, 1155, 1488, 1773
BclI	TGATCA	1 cut(s)	85
BcnI	CCSGG	2 cut(s)	253, 1141
BcoDI	GTCTC	6 cut(s)	22, 32, 1306, 1382, 1647, 2149
BcuI	ACTAGT	2 cut(s)	164, 1777
BfaI	CTAG	9 cut(s)	165, 281, 575, 776, 945, 990, 1094, 1163, 1778
BfoI	RGCGCY	1 cut(s)	2121
BfrI	CTTAAG	1 cut(s)	1846
BfuAI	ACCTGC	1 cut(s)	19
BmcAI	AGTACT	1 cut(s)	1559
Bme1390I	CCNGG	6 cut(s)	253, 744, 1141, 1155, 1488, 1773
Bme18I	GGWCC	4 cut(s)	479, 862, 935, 2123
BmgBI	CACGTC	2 cut(s)	1276, 1550
BmgT120I	GGNCC	6 cut(s)	361, 479, 862, 935, 1439, 2123
BmiI	GGNNCC	8 cut(s)	688, 864, 1160, 1240, 1262, 1485, 2119, 2125
BmrFI	CCNGG	6 cut(s)	253, 744, 1141, 1155, 1488, 1773
BmsI	GCATC	5 cut(s)	638, 781, 1256, 1510, 1703
BpiI	GAAGAC	1 cut(s)	1323
BpuEI	CTTGAG	3 cut(s)	350, 1212, 1920
BpuMI	CCSGG	2 cut(s)	253, 1141
BsaHI	GRCGYC	2 cut(s)	747, 2118
BsaI	GGTCTC	1 cut(s)	1647
BsaJI	CCNNGG	7 cut(s)	633, 676, 742, 891, 897, 968, 1154
BsaWI	WCCGGW	4 cut(s)	185, 445, 1571, 1859
BsaXI	ACNNNNNCTCC	4 cut(s)	315, 345, 1252, 1282
Bsc4I	CCNNNNNNNGG	1 cut(s)	892
Bse118I	RCCGGY	1 cut(s)	2120
Bse1I	ACTGG	1 cut(s)	1298
Bse3DI	GCAATG	2 cut(s)	688, 1242
BseAI	TCCGGA	1 cut(s)	445
BseBI	CCWGG	4 cut(s)	744, 1155, 1488, 1773
BseDI	CCNNGG	7 cut(s)	633, 676, 742, 891, 897, 968, 1154
BseGI	GGATG	3 cut(s)	629, 978, 1768
BseLI	CCNNNNNNNGG	1 cut(s)	892
BseMI	GCAATG	2 cut(s)	688, 1242
BseMII	CTCAG	1 cut(s)	579
BseNI	ACTGG	1 cut(s)	1298
BseRI	GAGGAG	3 cut(s)	20, 474, 872
BseSI	GKGCMC	2 cut(s)	124, 761
BseXI	GCAGC	8 cut(s)	260, 625, 697, 973, 1117, 1492, 1597, 1881
Bsh1236I	CGCG	2 cut(s)	872, 956
Bsh1285I	CGRYCG	3 cut(s)	201, 876, 954
BshFI	GGCC	8 cut(s)	363, 675, 896, 1440, 1684, 1721, 1743, 1999
BshNI	GGYRCC	3 cut(s)	1158, 1483, 2117
BsiEI	CGRYCG	3 cut(s)	201, 876, 954
BsiHKAI	GWGCWC	4 cut(s)	124, 556, 761, 1411
BslFI	GGGAC	4 cut(s)	139, 614, 624, 2109
BslI	CCNNNNNNNGG	1 cut(s)	892
BsmAI	GTCTC	6 cut(s)	22, 32, 1306, 1382, 1647, 2149
BsmFI	GGGAC	4 cut(s)	139, 614, 624, 2109
BsmI	GAATGC	1 cut(s)	1696
BsnI	GGCC	8 cut(s)	363, 675, 896, 1440, 1684, 1721, 1743, 1999
Bso31I	GGTCTC	1 cut(s)	1647
Bsp1286I	GDGCHC	4 cut(s)	124, 556, 761, 1411
Bsp13I	TCCGGA	1 cut(s)	445
Bsp19I	CCATGG	1 cut(s)	891
Bsp68I	TCGCGA	2 cut(s)	872, 956
BspANI	GGCC	8 cut(s)	363, 675, 896, 1440, 1684, 1721, 1743, 1999
BspCNI	CTCAG	1 cut(s)	580
BspEI	TCCGGA	1 cut(s)	445
BspFNI	CGCG	2 cut(s)	872, 956
BspHI	TCATGA	1 cut(s)	1668
BspLI	GGNNCC	8 cut(s)	688, 864, 1160, 1240, 1262, 1485, 2119, 2125
BspMI	ACCTGC	1 cut(s)	19
BspPI	GGATC	5 cut(s)	213, 625, 1498, 1648, 1745
BspT107I	GGYRCC	3 cut(s)	1158, 1483, 2117
BspTI	CTTAAG	1 cut(s)	1846
BspTNI	GGTCTC	1 cut(s)	1647
BsrDI	GCAATG	2 cut(s)	688, 1242
BsrFI	RCCGGY	1 cut(s)	2120
BsrI	ACTGG	1 cut(s)	1298
BssAI	RCCGGY	1 cut(s)	2120
BssECI	CCNNGG	7 cut(s)	633, 676, 742, 891, 897, 968, 1154
BssNAI	GTATAC	2 cut(s)	112, 1099
BssNI	GRCGYC	2 cut(s)	747, 2118
BssSI	CACGAG	2 cut(s)	132, 1434
BssT1I	CCWWGG	2 cut(s)	891, 897
Bst1107I	GTATAC	2 cut(s)	112, 1099
Bst2BI	CACGAG	2 cut(s)	132, 1434
Bst2UI	CCWGG	4 cut(s)	744, 1155, 1488, 1773
BstACI	GRCGYC	2 cut(s)	747, 2118
BstAFI	CTTAAG	1 cut(s)	1846
BstAPI	GCANNNNNTGC	2 cut(s)	128, 1875
BstC8I	GCNNGC	1 cut(s)	1741
BstDEI	CTNAG	1 cut(s)	588
BstDSI	CCRYGG	3 cut(s)	633, 891, 968
BstF5I	GGATG	3 cut(s)	629, 978, 1768
BstFNI	CGCG	2 cut(s)	872, 956
BstH2I	RGCGCY	1 cut(s)	2121
BstHHI	GCGC	2 cut(s)	967, 2120
BstMAI	GTCTC	6 cut(s)	22, 32, 1306, 1382, 1647, 2149
BstMCI	CGRYCG	3 cut(s)	201, 876, 954
BstNI	CCWGG	4 cut(s)	744, 1155, 1488, 1773
BstNSI	RCATGY	3 cut(s)	148, 662, 1598
BstSCI	CCNGG	6 cut(s)	251, 742, 1139, 1153, 1486, 1771
BstSLI	GKGCMC	2 cut(s)	124, 761
BstUI	CGCG	2 cut(s)	872, 956
BstV1I	GCAGC	8 cut(s)	260, 625, 697, 973, 1117, 1492, 1597, 1881
BstV2I	GAAGAC	1 cut(s)	1323
BstZ17I	GTATAC	2 cut(s)	112, 1099
BsuRI	GGCC	8 cut(s)	363, 675, 896, 1440, 1684, 1721, 1743, 1999
BtgI	CCRYGG	3 cut(s)	633, 891, 968
BtgZI	GCGATG	1 cut(s)	1792
BtrI	CACGTC	2 cut(s)	1276, 1550
BtsCI	GGATG	3 cut(s)	629, 978, 1768
BtsI	GCAGTG	1 cut(s)	1833
BtsIMutI	CAGTG	5 cut(s)	439, 937, 978, 1329, 1833
BtuMI	TCGCGA	2 cut(s)	872, 956
BveI	ACCTGC	1 cut(s)	19
Cac8I	GCNNGC	1 cut(s)	1741
CaiI	CAGNNNCTG	1 cut(s)	1304
CciI	TCATGA	1 cut(s)	1668
CfoI	GCGC	2 cut(s)	967, 2120
Cfr10I	RCCGGY	1 cut(s)	2120
Cfr13I	GGNCC	6 cut(s)	361, 479, 862, 935, 1439, 2123
CseI	GACGC	1 cut(s)	736
Csp6I	GTAC	6 cut(s)	381, 559, 1087, 1269, 1558, 2083
CviQI	GTAC	6 cut(s)	381, 559, 1087, 1269, 1558, 2083
DdeI	CTNAG	1 cut(s)	588
DinI	GGCGCC	1 cut(s)	2119
DraI	TTTAAA	1 cut(s)	2073
DraIII	CACNNNGTG	1 cut(s)	2182
DrdI	GACNNNNNNGTC	1 cut(s)	746
DseDI	GACNNNNNNGTC	1 cut(s)	746
EaeI	YGGCCR	4 cut(s)	894, 1682, 1719, 1741
EciI	GGCGGA	1 cut(s)	1126
Ecl136II	GAGCTC	1 cut(s)	1409
Eco130I	CCWWGG	2 cut(s)	891, 897
Eco147I	AGGCCT	1 cut(s)	1999
Eco24I	GRGCYC	1 cut(s)	1411
Eco31I	GGTCTC	1 cut(s)	1647
Eco47I	GGWCC	4 cut(s)	479, 862, 935, 2123
Eco53kI	GAGCTC	1 cut(s)	1409
Eco57I	CTGAAG	2 cut(s)	54, 347
EcoICRI	GAGCTC	1 cut(s)	1409
EcoO109I	RGGNCCY	1 cut(s)	862
EcoRI	GAATTC	2 cut(s)	771, 916
EcoRII	CCWGG	4 cut(s)	742, 1153, 1486, 1771
EcoT14I	CCWWGG	2 cut(s)	891, 897
EcoT22I	ATGCAT	1 cut(s)	1696
EcoT38I	GRGCYC	1 cut(s)	1411
EgeI	GGCGCC	1 cut(s)	2119
EheI	GGCGCC	1 cut(s)	2119
ErhI	CCWWGG	2 cut(s)	891, 897
FalI	AAGNNNNNCTT	2 cut(s)	2081, 2113
FaqI	GGGAC	4 cut(s)	139, 614, 624, 2109
FauI	CCCGC	1 cut(s)	1215
FbaI	TGATCA	1 cut(s)	85
FblI	GTMKAC	2 cut(s)	111, 1098
FokI	GGATG	3 cut(s)	616, 985, 1755
FriOI	GRGCYC	1 cut(s)	1411
FspBI	CTAG	9 cut(s)	165, 281, 575, 776, 945, 990, 1094, 1163, 1778
GlaI	GCGC	2 cut(s)	966, 2119
HaeII	RGCGCY	1 cut(s)	2121
HaeIII	GGCC	8 cut(s)	363, 675, 896, 1440, 1684, 1721, 1743, 1999
HgaI	GACGC	1 cut(s)	736
HhaI	GCGC	2 cut(s)	967, 2120
Hin1I	GRCGYC	2 cut(s)	747, 2118
Hin6I	GCGC	2 cut(s)	965, 2118
HinP1I	GCGC	2 cut(s)	965, 2118
HincII	GTYRAC	1 cut(s)	139
HindII	GTYRAC	1 cut(s)	139
HindIII	AAGCTT	1 cut(s)	542
HinfI	GANTC	8 cut(s)	243, 438, 449, 590, 903, 1202, 1360, 1851
HphI	GGTGA	5 cut(s)	175, 848, 1143, 1622, 1874
Hpy166II	GTNNAC	9 cut(s)	112, 122, 139, 759, 811, 978, 1099, 1172, 2176
Hpy188I	TCNGA	8 cut(s)	268, 454, 1031, 1380, 1495, 1540, 1801, 2167
Hpy8I	GTNNAC	9 cut(s)	112, 122, 139, 759, 811, 978, 1099, 1172, 2176
Hpy99I	CGWCG	3 cut(s)	752, 1418, 1994
HpyAV	CCTTC	2 cut(s)	1932, 2107
HpyCH4IV	ACGT	6 cut(s)	344, 379, 1174, 1275, 1549, 1989
HpyCH4V	TGCA	9 cut(s)	122, 510, 713, 759, 1247, 1694, 1763, 1838, 1878
HpyF3I	CTNAG	1 cut(s)	588
HpySE526I	ACGT	6 cut(s)	344, 379, 1174, 1275, 1549, 1989
Hsp92I	GRCGYC	2 cut(s)	747, 2118
HspAI	GCGC	2 cut(s)	965, 2118
KasI	GGCGCC	1 cut(s)	2117
Kpn2I	TCCGGA	1 cut(s)	445
Ksp22I	TGATCA	1 cut(s)	85
LmnI	GCTCC	6 cut(s)	336, 551, 1238, 1260, 1414, 1736
Lsp1109I	GCAGC	8 cut(s)	260, 625, 697, 973, 1117, 1492, 1597, 1881
LweI	GCATC	5 cut(s)	638, 781, 1256, 1510, 1703
MaeI	CTAG	9 cut(s)	165, 281, 575, 776, 945, 990, 1094, 1163, 1778
MaeII	ACGT	6 cut(s)	344, 379, 1174, 1275, 1549, 1989
MaeIII	GTNAC	5 cut(s)	181, 737, 1012, 1981, 2104
MboII	GAAGA	5 cut(s)	164, 1062, 1279, 1328, 2101
MhlI	GDGCHC	4 cut(s)	124, 556, 761, 1411
MlsI	TGGCCA	3 cut(s)	896, 1684, 1743
MluCI	AATT	6 cut(s)	220, 352, 771, 916, 1060, 1764
MluNI	TGGCCA	3 cut(s)	896, 1684, 1743
Mly113I	GGCGCC	1 cut(s)	2118
MlyI	GAGTC	2 cut(s)	599, 1845
MmeI	TCCRAC	2 cut(s)	1027, 1824
Mox20I	TGGCCA	3 cut(s)	896, 1684, 1743
Mph1103I	ATGCAT	1 cut(s)	1696
MroI	TCCGGA	1 cut(s)	445
MroXI	GAANNNNTTC	1 cut(s)	2096
MscI	TGGCCA	3 cut(s)	896, 1684, 1743
MseI	TTAA	7 cut(s)	1074, 1398, 1619, 1710, 1847, 1913, 2072
MslI	CAYNNNNRTG	2 cut(s)	149, 1156
Msp20I	TGGCCA	3 cut(s)	896, 1684, 1743
MspA1I	CMGCKG	2 cut(s)	710, 2155
MspCI	CTTAAG	1 cut(s)	1846
MspR9I	CCNGG	6 cut(s)	253, 744, 1141, 1155, 1488, 1773
Mva1269I	GAATGC	1 cut(s)	1696
MvaI	CCWGG	4 cut(s)	744, 1155, 1488, 1773
MvnI	CGCG	2 cut(s)	872, 956
NarI	GGCGCC	1 cut(s)	2118
NciI	CCSGG	2 cut(s)	253, 1141
NcoI	CCATGG	1 cut(s)	891
NlaIV	GGNNCC	8 cut(s)	688, 864, 1160, 1240, 1262, 1485, 2119, 2125
NmeAIII	GCCGAG	2 cut(s)	657, 1747
NmuCI	GTSAC	4 cut(s)	181, 737, 1012, 1981
NruI	TCGCGA	2 cut(s)	872, 956
NsiI	ATGCAT	1 cut(s)	1696
NspI	RCATGY	3 cut(s)	148, 662, 1598
OliI	CACNNNNGTG	1 cut(s)	1156
PagI	TCATGA	1 cut(s)	1668
PceI	AGGCCT	1 cut(s)	1999
PcsI	WCGNNNNNNNCGW	4 cut(s)	385, 426, 1061, 1719
PctI	GAATGC	1 cut(s)	1696
PdmI	GAANNNNTTC	1 cut(s)	2096
PfeI	GAWTC	6 cut(s)	243, 438, 449, 903, 1202, 1360
PflMI	CCANNNNNTGG	1 cut(s)	892
PfoI	TCCNGGA	1 cut(s)	1771
Ple19I	CGATCG	2 cut(s)	201, 876
PleI	GAGTC	2 cut(s)	598, 1845
PluTI	GGCGCC	1 cut(s)	2121
PpsI	GAGTC	2 cut(s)	598, 1845
PpuMI	RGGWCCY	1 cut(s)	862
PsiI	TTATAA	2 cut(s)	225, 729
Psp124BI	GAGCTC	1 cut(s)	1411
Psp5II	RGGWCCY	1 cut(s)	862
Psp6I	CCWGG	4 cut(s)	742, 1153, 1486, 1771
PspGI	CCWGG	4 cut(s)	742, 1153, 1486, 1771
PspN4I	GGNNCC	8 cut(s)	688, 864, 1160, 1240, 1262, 1485, 2119, 2125
PspPI	GGNCC	6 cut(s)	361, 479, 862, 935, 1439, 2123
PspPPI	RGGWCCY	1 cut(s)	862
PstNI	CAGNNNCTG	1 cut(s)	1304
PvuI	CGATCG	2 cut(s)	201, 876
PvuII	CAGCTG	2 cut(s)	710, 2155
RruI	TCGCGA	2 cut(s)	872, 956
RsaI	GTAC	6 cut(s)	382, 560, 1088, 1270, 1559, 2084
RsaNI	GTAC	6 cut(s)	381, 559, 1087, 1269, 1558, 2083
RseI	CAYNNNNRTG	2 cut(s)	149, 1156
SacI	GAGCTC	1 cut(s)	1411
SaqAI	TTAA	7 cut(s)	1074, 1398, 1619, 1710, 1847, 1913, 2072
Sau96I	GGNCC	6 cut(s)	361, 479, 862, 935, 1439, 2123
ScaI	AGTACT	1 cut(s)	1559
SchI	GAGTC	2 cut(s)	599, 1845
ScrFI	CCNGG	6 cut(s)	253, 744, 1141, 1155, 1488, 1773
SduI	GDGCHC	4 cut(s)	124, 556, 761, 1411
SfaNI	GCATC	5 cut(s)	638, 781, 1256, 1510, 1703
SfoI	GGCGCC	1 cut(s)	2119
SinI	GGWCC	4 cut(s)	479, 862, 935, 2123
SmiMI	CAYNNNNRTG	2 cut(s)	149, 1156
SmlI	CTYRAG	4 cut(s)	365, 1227, 1846, 1935
SmoI	CTYRAG	4 cut(s)	365, 1227, 1846, 1935
SpeI	ACTAGT	2 cut(s)	164, 1777
Sse9I	AATT	6 cut(s)	220, 352, 771, 916, 1060, 1764
SseBI	AGGCCT	1 cut(s)	1999
SspDI	GGCGCC	1 cut(s)	2117
SspMI	CTAG	9 cut(s)	165, 281, 575, 776, 945, 990, 1094, 1163, 1778
SstI	GAGCTC	1 cut(s)	1411
StuI	AGGCCT	1 cut(s)	1999
StyD4I	CCNGG	6 cut(s)	251, 742, 1139, 1153, 1486, 1771
StyI	CCWWGG	2 cut(s)	891, 897
TaiI	ACGT	6 cut(s)	347, 382, 1177, 1278, 1552, 1992
TaqI	TCGA	7 cut(s)	197, 201, 429, 750, 1068, 1749, 1925
TasI	AATT	6 cut(s)	220, 352, 771, 916, 1060, 1764
TatI	WGTACW	1 cut(s)	1557
TauI	GCSGC	5 cut(s)	519, 782, 1373, 1824, 1949
TfiI	GAWTC	6 cut(s)	243, 438, 449, 903, 1202, 1360
Tru1I	TTAA	7 cut(s)	1074, 1398, 1619, 1710, 1847, 1913, 2072
Tru9I	TTAA	7 cut(s)	1074, 1398, 1619, 1710, 1847, 1913, 2072
TscAI	CASTG	5 cut(s)	439, 937, 985, 1336, 1840
TseFI	GTSAC	4 cut(s)	181, 737, 1012, 1981
TseI	GCWGC	8 cut(s)	248, 638, 710, 986, 1130, 1505, 1610, 1869
Tsp45I	GTSAC	4 cut(s)	181, 737, 1012, 1981
TspDTI	ATGAA	6 cut(s)	346, 1236, 1361, 1703, 1757, 1800
TspGWI	ACGGA	6 cut(s)	255, 783, 1092, 1552, 1749, 1961
TspRI	CASTG	5 cut(s)	439, 937, 985, 1336, 1840
Van91I	CCANNNNNTGG	1 cut(s)	892
Vha464I	CTTAAG	1 cut(s)	1846
VneI	GTGCAC	2 cut(s)	120, 757
VpaK11BI	GGWCC	4 cut(s)	479, 862, 935, 2123
XapI	RAATTY	4 cut(s)	352, 771, 916, 1060
XceI	RCATGY	3 cut(s)	148, 662, 1598
XmiI	GTMKAC	2 cut(s)	111, 1098
XmnI	GAANNNNTTC	1 cut(s)	2096
XspI	CTAG	9 cut(s)	165, 281, 575, 776, 945, 990, 1094, 1163, 1778
ZrmI	AGTACT	1 cut(s)	1559
Zsp2I	ATGCAT	1 cut(s)	1696

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.