MD07G1037600.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000257
Physical Location & Seq
Reverse (-)
3080939 .. 3083137
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1037600.v1.1.491

Sequence Viewer

Length: 2199 bp
ATGGCTAAGTATGGAGCTCTCTTGTGTTCTTATGCTTTCCCTATTCTTATACTCACTATCAGCTTCTTCAGCAAAGCCATTGGTCAAGAAGATAGAAAGGTTCACATTGTGTACATGGGGTCACTTCCGACTGTTGAATTGTACTCATCGTTGTCTCACCACCTCGGTATGCTAGAAAGAGTTGTCGAGGGAAGTTCTGCTGCAAATGTCTTGGTCAGAAGTTACGGAAGGAGTTTCAATGGATTTGCTGCCAAGCTCACCGAGCGCGAAAGAGAAAAGCTTGCTAACATGAAGGAAGTGGTCTCTGTGTTTCCAAGCAGAACTTACCAACTTCAAACAACAAGGTCTTGGGACTTTATGGGTTTACCAGAGAACGTCACTCTAAATACCACCAGTGGCGGTGAGAGTGATATTGTTGTCGGCGTGATTGACACCGGAATTTGGCCTGAATCCAATAGCTTTAGAGATGATGGGTTTGGTCCTCCTCCCAAGAGTTGGAAAGGTGCTTGTGAAGGTGGCCAAAATTTTAATTGCAACAACAAGATCATTGGAGCTCGATATTACACATTAGAGGAGTCTGCAAGGGATGTAATAGGTCATGGAAGCCACACTGCCTCAACGGCAGCAGGTCGTGCCGTGAAGAATGTGAGCTTTTATGGACTAGGCAATGGTACTGCAAGAGGAGGAGCTCCTGGTGCGAGAATCGCGGCGTATAAAGTCTGCACTGATCAAGGATGCACTTCGGAGAATATCTTGGCTGCTTTCGATGATTGTACTGCTGATGAAGTCAGCATTATTTCAGTTTCACTTGGAAGTCCTTACGCGCCTCCTATGGAAGATGATCCTATTGCCATTGGTGCCTTTCATGCAATGAAGAAAGGGATAATGACCATACAATCTGCTGGCAACAGTGGCCCTGGAGAGGGTACTGTATCAAGTACACCACCATGGATTTTTACGGTTGCAGCATGTAGCATCGATCGTAAATTCATTGCCAAGGTTTTTCTTGGAAATGGACGTACATTGGATGGGCTAGCCGTGAATGCTTTTGAATCAAATGGAACAAGTGCACCTTTGATATATGGAAGAGAGGCTTCGAGTCACTGCTCAGAATACCCTGCACAGGATTGTGCAGAAGGCTGCCTAGACCCTGACAGAGTCAAGGGAAAATATGTGATGTGTGATAACTTTGGAGGACTAATTGAGGCTGATCGAGCTGGTGCAAGAGGTGCGGTTTTAAAGAATGCAAGAGATGATGTTCCTTTTGTTCTCCCACGATCTATGAGCGGTGTAAACAACAGTGATTATAATCTGGCCAAATCCTACCAGAACTCCACCACAAATCCTCGAGCAAATATAGTAAAAAGTGAAGTCATAAAAGATCCTACCGCACCAAGTGTCGCTTCCTTCTCTTCGCGTGGTCCTAATCCAATTTTACCAGAAATTTTGAAGCCGGATATTTGTGCCCCAGGAATAGCTATTTTGGCTGCATATTCTCCAAATGCTTCTGCCTCAGCCAGCCCTTATGACCAGAGGCGCGTAAAGTACAATGTACTATCTGGAACCTCCATGTCTTGCCCCCATGCTGCTGGTGCTGCTGCATACGTTAAAGAATTCCACCCGGATTGGTCTCCAGCAGCCATCAAGTCAGCTCTTATGACTACAGCTTGGCCCATGAATGATACAAGCACTTCCCCAGGCGAATTTGGTTATGGAACTGGACATCTCAATCCTGTAAGAGCTAAGCAGCCGGGACTTGTGTACGATGCTTCTAAAGAAGATTACATTAAGTTGCTTTGCATGTTTTATAAGGATGAGACTCGAGTTAGACTCATCTCGGGAGATAACAGCACGTGCCCTACTGGCTCCGACAAAGGAACACCAAAGGATCACAATTACCCTTCAATGCTTGCTAATGTAACATCAAGGGCATCATTTAAGGTTGAATTTCCTAGAAGAGTTACGAATGTTGGCCATGCAAACTCCACATACAAGGCAAGTATCTCCAGGAATTCTGAAAATGCCGACATCAAAGTGGTGCCTGAAGTCCTTTACTTCAAATCCTTGAACGAGGTGAAAAGTTTCGTTGTGACCGTTGCTGGACCAGGCCTGTCCGTGGCTGGAATTGTGACAGCGTCACTGACTTGGTCTGATGGAAGTCACCACGTTACAAGTCCAATTATTGTACGGGGAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

733

Amino Acids

78.13

Weight (kDa)

7.51

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 112 4.6e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 572 4e-45 Subtilase family
fn3_6 PF17766 630 - 729 5.1e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1308, 1809
Acc36I ACCTGC 1 cut(s) 617
AccB1I GGYRCC 2 cut(s) 857, 2038
AccB7I CCANNNNNTGG 1 cut(s) 495
AccBSI CCGCTC 1 cut(s) 1287
AccII CGCG 5 cut(s) 267, 707, 824, 1417, 1539
AciI CCGC 5 cut(s) 399, 707, 1232, 1287, 1389
AclWI GGATC 3 cut(s) 836, 1376, 1896
AcoI YGGCCR 3 cut(s) 517, 1314, 1972
AcsI RAATTY 8 cut(s) 438, 523, 986, 1443, 1613, 1703, 1946, 2011
AcuI CTGAAG 2 cut(s) 52, 2064
AcvI CACGTG 1 cut(s) 1854
AdeI CACNNNGTG 2 cut(s) 109, 1397
AfiI CCNNNNNNNGG 6 cut(s) 441, 495, 922, 923, 1123, 2116
AgsI TTSAA 9 cut(s) 137, 238, 335, 1052, 1450, 1905, 1946, 2059, 2068
AjnI CCWGG 6 cut(s) 691, 916, 1468, 1696, 2006, 2104
AjuI GAANNNNNNNTTGG 2 cut(s) 1387, 1419
Alw21I GWGCWC 4 cut(s) 19, 556, 691, 1072
Alw26I GTCTC 4 cut(s) 159, 307, 1635, 1811
Alw44I GTGCAC 1 cut(s) 1068
AlwI GGATC 3 cut(s) 836, 1376, 1896
Ama87I CYCGRG 3 cut(s) 1347, 1821, 1837
AoxI GGCC 7 cut(s) 443, 517, 913, 1314, 1670, 1972, 2107
ApaLI GTGCAC 1 cut(s) 1068
ApoI RAATTY 8 cut(s) 438, 523, 986, 1443, 1613, 1703, 1946, 2011
Asp700I GAANNNNTTC 1 cut(s) 2081
AspLEI GCGC 3 cut(s) 267, 826, 1539
AspS9I GGNCC 5 cut(s) 479, 914, 1421, 1671, 2102
AsuC2I CCSGG 2 cut(s) 1622, 1752
AsuHPI GGTGA 5 cut(s) 149, 250, 413, 2086, 2153
AsuNHI GCTAGC 1 cut(s) 1033
AvaI CYCGRG 3 cut(s) 1347, 1821, 1837
AvaII GGWCC 3 cut(s) 479, 1421, 2102
BaeGI GKGCMC 3 cut(s) 1072, 1468, 1859
BalI TGGCCA 3 cut(s) 519, 1316, 1974
BanI GGYRCC 2 cut(s) 857, 2038
BanII GRGCYC 3 cut(s) 19, 556, 691
BbrPI CACGTG 1 cut(s) 1854
Bbv12I GWGCWC 4 cut(s) 19, 556, 691, 1072
BbvCI CCTCAGC 1 cut(s) 1513
BccI CCATC 4 cut(s) 464, 1022, 1649, 2147
BceAI ACGGC 3 cut(s) 620, 636, 1022
BciT130I CCWGG 6 cut(s) 693, 918, 1470, 1698, 2008, 2106
BclI TGATCA 1 cut(s) 727
BcnI CCSGG 2 cut(s) 1622, 1752
BcoDI GTCTC 4 cut(s) 159, 307, 1635, 1811
BfaI CTAG 5 cut(s) 173, 662, 1034, 1145, 1953
BfmI CTRYAG 1 cut(s) 1662
BfuAI ACCTGC 1 cut(s) 617
BglI GCCNNNNNGGC 2 cut(s) 620, 1863
BlpI GCTNAGC 1 cut(s) 1743
Bme1390I CCNGG 8 cut(s) 693, 918, 1470, 1622, 1698, 1752, 2008, 2106
Bme18I GGWCC 3 cut(s) 479, 1421, 2102
BmeT110I CYCGRG 3 cut(s) 1347, 1821, 1837
BmgT120I GGNCC 5 cut(s) 479, 914, 1421, 1671, 2102
BmiI GGNNCC 4 cut(s) 859, 1564, 1867, 2040
BmrFI CCNGG 8 cut(s) 693, 918, 1470, 1622, 1698, 1752, 2008, 2106
BmsI GCATC 4 cut(s) 725, 984, 1756, 1940
BmtI GCTAGC 1 cut(s) 1037
BplI GAGNNNNNCTC 2 cut(s) 1815, 1847
BpmI CTGGAG 3 cut(s) 939, 1617, 1990
Bpu10I CCTNAGC 1 cut(s) 1513
Bpu1102I GCTNAGC 1 cut(s) 1743
BpuMI CCSGG 2 cut(s) 1622, 1752
Bsa29I ATCGAT 1 cut(s) 978
BsaAI YACGTR 1 cut(s) 1854
BsaBI GATNNNNATC 1 cut(s) 1308
BsaI GGTCTC 2 cut(s) 307, 1635
BsaJI CCNNGG 7 cut(s) 163, 916, 947, 996, 1468, 1696, 2115
BsaWI WCCGGW 1 cut(s) 434
BsaXI ACNNNNNCTCC 2 cut(s) 1316, 1346
Bsc4I CCNNNNNNNGG 6 cut(s) 441, 495, 922, 923, 1123, 2116
Bse1I ACTGG 3 cut(s) 393, 1723, 1867
Bse3DI GCAATG 3 cut(s) 673, 876, 990
Bse8I GATNNNNATC 1 cut(s) 1308
BseBI CCWGG 6 cut(s) 693, 918, 1470, 1698, 2008, 2106
BseCI ATCGAT 1 cut(s) 978
BseDI CCNNGG 7 cut(s) 163, 916, 947, 996, 1468, 1696, 2115
BseGI GGATG 4 cut(s) 592, 740, 1033, 1819
BseJI GATNNNNATC 1 cut(s) 1308
BseLI CCNNNNNNNGG 6 cut(s) 441, 495, 922, 923, 1123, 2116
BseMI GCAATG 3 cut(s) 673, 876, 990
BseMII CTCAG 2 cut(s) 1122, 1527
BseNI ACTGG 3 cut(s) 393, 1723, 1867
BseRI GAGGAG 4 cut(s) 474, 587, 696, 699
BseSI GKGCMC 3 cut(s) 1072, 1468, 1859
BsgI GTGCAG 3 cut(s) 706, 1104, 1152
Bsh1236I CGCG 5 cut(s) 267, 707, 824, 1417, 1539
Bsh1285I CGRYCG 1 cut(s) 982
BshFI GGCC 7 cut(s) 445, 519, 915, 1316, 1672, 1974, 2109
BshNI GGYRCC 2 cut(s) 857, 2038
BshVI ATCGAT 1 cut(s) 978
BsiEI CGRYCG 1 cut(s) 982
BsiHKAI GWGCWC 4 cut(s) 19, 556, 691, 1072
BsiHKCI CYCGRG 3 cut(s) 1347, 1821, 1837
BsiSI CCGG 4 cut(s) 435, 1454, 1622, 1751
BslFI GGGAC 2 cut(s) 365, 1767
BslI CCNNNNNNNGG 6 cut(s) 441, 495, 922, 923, 1123, 2116
BsmAI GTCTC 4 cut(s) 159, 307, 1635, 1811
BsmFI GGGAC 2 cut(s) 365, 1767
BsmI GAATGC 2 cut(s) 1048, 1249
BsnI GGCC 7 cut(s) 445, 519, 915, 1316, 1672, 1974, 2109
Bso31I GGTCTC 2 cut(s) 307, 1635
BsoBI CYCGRG 3 cut(s) 1347, 1821, 1837
Bsp1286I GDGCHC 6 cut(s) 19, 556, 691, 1072, 1468, 1859
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 8 cut(s) 543, 727, 841, 979, 1210, 1277, 1381, 1888
Bsp1720I GCTNAGC 1 cut(s) 1743
Bsp19I CCATGG 1 cut(s) 947
BspACI CCGC 5 cut(s) 399, 707, 1232, 1287, 1389
BspANI GGCC 7 cut(s) 445, 519, 915, 1316, 1672, 1974, 2109
BspCNI CTCAG 2 cut(s) 1121, 1526
BspDI ATCGAT 1 cut(s) 978
BspFNI CGCG 5 cut(s) 267, 707, 824, 1417, 1539
BspLI GGNNCC 4 cut(s) 859, 1564, 1867, 2040
BspMI ACCTGC 1 cut(s) 617
BspOI GCTAGC 1 cut(s) 1037
BspPI GGATC 3 cut(s) 836, 1376, 1896
BspT107I GGYRCC 2 cut(s) 857, 2038
BspTNI GGTCTC 2 cut(s) 307, 1635
BsrBI CCGCTC 1 cut(s) 1287
BsrDI GCAATG 3 cut(s) 673, 876, 990
BsrGI TGTACA 1 cut(s) 111
BsrI ACTGG 3 cut(s) 393, 1723, 1867
BssECI CCNNGG 7 cut(s) 163, 916, 947, 996, 1468, 1696, 2115
BssMI GATC 8 cut(s) 543, 727, 841, 979, 1210, 1277, 1381, 1888
BssT1I CCWWGG 2 cut(s) 947, 996
Bst2UI CCWGG 6 cut(s) 693, 918, 1470, 1698, 2008, 2106
Bst4CI ACNGT 6 cut(s) 133, 911, 931, 961, 1301, 2095
Bst6I CTCTTC 3 cut(s) 1081, 1417, 1951
BstAPI GCANNNNNTGC 2 cut(s) 632, 1229
BstAUI TGTACA 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 1854
BstC8I GCNNGC 5 cut(s) 282, 904, 1035, 1519, 1911
BstDEI CTNAG 4 cut(s) 6, 1108, 1513, 1743
BstDSI CCRYGG 2 cut(s) 947, 2115
BstF5I GGATG 4 cut(s) 592, 740, 1033, 1819
BstFNI CGCG 5 cut(s) 267, 707, 824, 1417, 1539
BstHHI GCGC 3 cut(s) 267, 826, 1539
BstKTI GATC 8 cut(s) 546, 730, 844, 982, 1213, 1280, 1384, 1891
BstMAI GTCTC 4 cut(s) 159, 307, 1635, 1811
BstMBI GATC 8 cut(s) 543, 727, 841, 979, 1210, 1277, 1381, 1888
BstMCI CGRYCG 1 cut(s) 982
BstNI CCWGG 6 cut(s) 693, 918, 1470, 1698, 2008, 2106
BstNSI RCATGY 2 cut(s) 972, 1804
BstSCI CCNGG 8 cut(s) 691, 916, 1468, 1620, 1696, 1750, 2006, 2104
BstSFI CTRYAG 1 cut(s) 1662
BstSLI GKGCMC 3 cut(s) 1072, 1468, 1859
BstUI CGCG 5 cut(s) 267, 707, 824, 1417, 1539
BstX2I RGATCY 1 cut(s) 1381
BstXI CCANNNNNNTGG 1 cut(s) 1589
BstYI RGATCY 1 cut(s) 1381
Bsu15I ATCGAT 1 cut(s) 978
BsuRI GGCC 7 cut(s) 445, 519, 915, 1316, 1672, 1974, 2109
BsuTUI ATCGAT 1 cut(s) 978
BtgI CCRYGG 2 cut(s) 947, 2115
BtsCI GGATG 4 cut(s) 592, 740, 1033, 1819
BtsI GCAGTG 2 cut(s) 609, 1102
BtsIMutI CAGTG 7 cut(s) 400, 609, 723, 916, 1102, 1306, 2138
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 5 cut(s) 282, 904, 1035, 1519, 1911
CfoI GCGC 3 cut(s) 267, 826, 1539
Cfr13I GGNCC 5 cut(s) 479, 914, 1421, 1671, 2102
ClaI ATCGAT 1 cut(s) 978
CseI GACGC 1 cut(s) 2124
CspCI CAANNNNNGTGG 2 cut(s) 1607, 1642
DdeI CTNAG 4 cut(s) 6, 1108, 1513, 1743
DpnI GATC 8 cut(s) 545, 729, 843, 981, 1212, 1279, 1383, 1890
DpnII GATC 8 cut(s) 543, 727, 841, 979, 1210, 1277, 1381, 1888
DraI TTTAAA 1 cut(s) 1239
DraIII CACNNNGTG 2 cut(s) 109, 1397
EaeI YGGCCR 3 cut(s) 517, 1314, 1972
Eam1104I CTCTTC 3 cut(s) 1081, 1417, 1951
EarI CTCTTC 3 cut(s) 1081, 1417, 1951
Ecl136II GAGCTC 3 cut(s) 17, 554, 689
Eco130I CCWWGG 2 cut(s) 947, 996
Eco147I AGGCCT 1 cut(s) 2109
Eco24I GRGCYC 3 cut(s) 19, 556, 691
Eco31I GGTCTC 2 cut(s) 307, 1635
Eco47I GGWCC 3 cut(s) 479, 1421, 2102
Eco53kI GAGCTC 3 cut(s) 17, 554, 689
Eco57I CTGAAG 2 cut(s) 52, 2064
Eco72I CACGTG 1 cut(s) 1854
Eco88I CYCGRG 3 cut(s) 1347, 1821, 1837
EcoICRI GAGCTC 3 cut(s) 17, 554, 689
EcoRI GAATTC 2 cut(s) 1613, 2011
EcoRII CCWGG 6 cut(s) 691, 916, 1468, 1696, 2006, 2104
EcoT14I CCWWGG 2 cut(s) 947, 996
EcoT38I GRGCYC 3 cut(s) 19, 556, 691
ErhI CCWWGG 2 cut(s) 947, 996
FalI AAGNNNNNCTT 8 cut(s) 307, 339, 1057, 1089, 1078, 1110, 1387, 1419
FaqI GGGAC 2 cut(s) 365, 1767
FbaI TGATCA 1 cut(s) 727
FokI GGATG 4 cut(s) 599, 747, 1040, 1826
FriOI GRGCYC 3 cut(s) 19, 556, 691
FspBI CTAG 5 cut(s) 173, 662, 1034, 1145, 1953
GlaI GCGC 3 cut(s) 266, 825, 1538
GsuI CTGGAG 3 cut(s) 939, 1617, 1990
HaeIII GGCC 7 cut(s) 445, 519, 915, 1316, 1672, 1974, 2109
HapII CCGG 4 cut(s) 435, 1454, 1622, 1751
HgaI GACGC 1 cut(s) 2124
HhaI GCGC 3 cut(s) 267, 826, 1539
Hin6I GCGC 3 cut(s) 265, 824, 1537
HinP1I GCGC 3 cut(s) 265, 824, 1537
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 8 cut(s) 449, 575, 702, 1052, 1099, 1158, 1819, 1830
HpaII CCGG 4 cut(s) 435, 1454, 1622, 1751
HphI GGTGA 5 cut(s) 149, 250, 413, 2086, 2153
Hpy166II GTNNAC 7 cut(s) 103, 112, 365, 941, 1070, 1294, 1762
Hpy188I TCNGA 7 cut(s) 129, 218, 745, 1111, 1870, 2017, 2152
Hpy188III TCNNGA 3 cut(s) 86, 1560, 1839
Hpy8I GTNNAC 7 cut(s) 103, 112, 365, 941, 1070, 1294, 1762
HpyAV CCTTC 6 cut(s) 222, 286, 506, 1130, 1417, 1911
HpyCH4III ACNGT 6 cut(s) 133, 911, 931, 961, 1301, 2095
HpyCH4IV ACGT 5 cut(s) 375, 1018, 1605, 1853, 2166
HpyF3I CTNAG 4 cut(s) 6, 1108, 1513, 1743
HpySE526I ACGT 5 cut(s) 375, 1018, 1605, 1853, 2166
HspAI GCGC 3 cut(s) 265, 824, 1537
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 8 cut(s) 543, 727, 841, 979, 1210, 1277, 1381, 1888
LmnI GCTCC 5 cut(s) 14, 551, 686, 694, 1871
LweI GCATC 4 cut(s) 725, 984, 1756, 1940
MaeI CTAG 5 cut(s) 173, 662, 1034, 1145, 1953
MaeII ACGT 5 cut(s) 375, 1018, 1605, 1853, 2166
MalI GATC 8 cut(s) 545, 729, 843, 981, 1212, 1279, 1383, 1890
MbiI CCGCTC 1 cut(s) 1287
MboI GATC 8 cut(s) 543, 727, 841, 979, 1210, 1277, 1381, 1888
MboII GAAGA 9 cut(s) 58, 101, 652, 848, 886, 1098, 1404, 1790, 1968
MflI RGATCY 1 cut(s) 1381
MhlI GDGCHC 6 cut(s) 19, 556, 691, 1072, 1468, 1859
MlsI TGGCCA 3 cut(s) 519, 1316, 1974
MluNI TGGCCA 3 cut(s) 519, 1316, 1974
MlyI GAGTC 5 cut(s) 584, 1108, 1167, 1813, 1824
MmeI TCCRAC 3 cut(s) 152, 476, 1893
Mox20I TGGCCA 3 cut(s) 519, 1316, 1974
MroXI GAANNNNTTC 1 cut(s) 2081
MscI TGGCCA 3 cut(s) 519, 1316, 1974
MseI TTAA 5 cut(s) 528, 1238, 1608, 1788, 1938
MslI CAYNNNNRTG 2 cut(s) 946, 2033
Msp20I TGGCCA 3 cut(s) 519, 1316, 1974
MspI CCGG 4 cut(s) 435, 1454, 1622, 1751
MspR9I CCNGG 8 cut(s) 693, 918, 1470, 1622, 1698, 1752, 2008, 2106
Mva1269I GAATGC 2 cut(s) 1048, 1249
MvaI CCWGG 6 cut(s) 693, 918, 1470, 1698, 2008, 2106
MvnI CGCG 5 cut(s) 267, 707, 824, 1417, 1539
NciI CCSGG 2 cut(s) 1622, 1752
NcoI CCATGG 1 cut(s) 947
NdeII GATC 8 cut(s) 543, 727, 841, 979, 1210, 1277, 1381, 1888
NheI GCTAGC 1 cut(s) 1033
NlaIV GGNNCC 4 cut(s) 859, 1564, 1867, 2040
NmuCI GTSAC 7 cut(s) 120, 376, 1100, 2089, 2128, 2136, 2159
NspI RCATGY 2 cut(s) 972, 1804
PaeR7I CTCGAG 2 cut(s) 1347, 1821
PceI AGGCCT 1 cut(s) 2109
PcsI WCGNNNNNNNCGW 1 cut(s) 2091
PctI GAATGC 2 cut(s) 1048, 1249
PdmI GAANNNNTTC 1 cut(s) 2081
PfeI GAWTC 3 cut(s) 449, 702, 1052
PflFI GACNNNGTC 3 cut(s) 1157, 2134, 2146
PflMI CCANNNNNTGG 1 cut(s) 495
PfoI TCCNGGA 1 cut(s) 2006
Ple19I CGATCG 1 cut(s) 982
PleI GAGTC 5 cut(s) 583, 1107, 1166, 1813, 1824
PmaCI CACGTG 1 cut(s) 1854
PmlI CACGTG 1 cut(s) 1854
PpsI GAGTC 5 cut(s) 583, 1107, 1166, 1813, 1824
Ppu21I YACGTR 1 cut(s) 1854
PsiI TTATAA 2 cut(s) 1308, 1809
Psp124BI GAGCTC 3 cut(s) 19, 556, 691
Psp6I CCWGG 6 cut(s) 691, 916, 1468, 1696, 2006, 2104
PspCI CACGTG 1 cut(s) 1854
PspGI CCWGG 6 cut(s) 691, 916, 1468, 1696, 2006, 2104
PspN4I GGNNCC 4 cut(s) 859, 1564, 1867, 2040
PspPI GGNCC 5 cut(s) 479, 914, 1421, 1671, 2102
PspXI VCTCGAGB 2 cut(s) 1347, 1821
PsuI RGATCY 1 cut(s) 1381
PsyI GACNNNGTC 3 cut(s) 1157, 2134, 2146
PvuI CGATCG 1 cut(s) 982
RseI CAYNNNNRTG 2 cut(s) 946, 2033
SacI GAGCTC 3 cut(s) 19, 556, 691
SaqAI TTAA 5 cut(s) 528, 1238, 1608, 1788, 1938
Sau3AI GATC 8 cut(s) 543, 727, 841, 979, 1210, 1277, 1381, 1888
Sau96I GGNCC 5 cut(s) 479, 914, 1421, 1671, 2102
SchI GAGTC 5 cut(s) 584, 1108, 1167, 1813, 1824
ScrFI CCNGG 8 cut(s) 693, 918, 1470, 1622, 1698, 1752, 2008, 2106
SduI GDGCHC 6 cut(s) 19, 556, 691, 1072, 1468, 1859
SfaNI GCATC 4 cut(s) 725, 984, 1756, 1940
SfcI CTRYAG 1 cut(s) 1662
Sfr274I CTCGAG 2 cut(s) 1347, 1821
SinI GGWCC 3 cut(s) 479, 1421, 2102
SlaI CTCGAG 2 cut(s) 1347, 1821
SmiMI CAYNNNNRTG 2 cut(s) 946, 2033
SmlI CTYRAG 2 cut(s) 1347, 1821
SmoI CTYRAG 2 cut(s) 1347, 1821
SseBI AGGCCT 1 cut(s) 2109
SsiI CCGC 5 cut(s) 399, 707, 1232, 1287, 1389
SspMI CTAG 5 cut(s) 173, 662, 1034, 1145, 1953
SstI GAGCTC 3 cut(s) 19, 556, 691
StuI AGGCCT 1 cut(s) 2109
StyD4I CCNGG 8 cut(s) 691, 916, 1468, 1620, 1696, 1750, 2006, 2104
StyI CCWWGG 2 cut(s) 947, 996
TaaI ACNGT 6 cut(s) 133, 911, 931, 961, 1301, 2095
TaiI ACGT 5 cut(s) 378, 1021, 1608, 1856, 2169
TaqI TCGA 8 cut(s) 186, 556, 765, 978, 1097, 1213, 1348, 1822
TatI WGTACW 6 cut(s) 111, 141, 773, 938, 1545, 1552
TauI GCSGC 1 cut(s) 710
TfiI GAWTC 3 cut(s) 449, 702, 1052
Tru1I TTAA 5 cut(s) 528, 1238, 1608, 1788, 1938
Tru9I TTAA 5 cut(s) 528, 1238, 1608, 1788, 1938
TscAI CASTG 7 cut(s) 400, 616, 730, 916, 1109, 1306, 2145
TseFI GTSAC 7 cut(s) 120, 376, 1100, 2089, 2128, 2136, 2159
Tsp45I GTSAC 7 cut(s) 120, 376, 1100, 2089, 2128, 2136, 2159
TspDTI ATGAA 6 cut(s) 305, 798, 854, 887, 979, 1691
TspGWI ACGGA 2 cut(s) 240, 2104
TspRI CASTG 7 cut(s) 400, 616, 730, 916, 1109, 1306, 2145
Tth111I GACNNNGTC 3 cut(s) 1157, 2134, 2146
Van91I CCANNNNNTGG 1 cut(s) 495
VneI GTGCAC 1 cut(s) 1068
VpaK11BI GGWCC 3 cut(s) 479, 1421, 2102
XapI RAATTY 8 cut(s) 438, 523, 986, 1443, 1613, 1703, 1946, 2011
XceI RCATGY 2 cut(s) 972, 1804
XhoI CTCGAG 2 cut(s) 1347, 1821
XmnI GAANNNNTTC 1 cut(s) 2081
XspI CTAG 5 cut(s) 173, 662, 1034, 1145, 1953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.