RLG00000030338

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000257
Physical Location & Seq
Forward (+)
59789507 .. 59791593
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030338

Sequence Viewer

Length: 1674 bp
ATGGCAAAGCATAGAGCTCTCCAGGTTTCCTATGTTTGCCCCACTCTTATACTAACCTTGAGCTTCTTCTTCTGCAAAGCCATCGATGAAAACAGAAAGCTCCATATCGTGTACCTGGGGTCACTTCCTGATAGTGAGGTGTACTCACCATTGTCTCACCATCTTGGTATACTAGAAAGAGTTGTCGAGAGCAACTCCACTTCCAATTTTCTTACAAGAAACTTCAATGGATTTGCTGCATACCTCACTGACCGGGAAAGGGAAAGGCTTGCTAGCATGAAAGAAGTAGTCTCTGTATTTCCAAGCACAGCTTTCCAACTTCAAACGACAAGATCTTGGGACTTTCTTGGTTTCAATGAGAAAATCCAACGAAATGACAGCGTTGAGAGTAATATCATTATTGGTGTCATTGACTCTGGAATTTGGCCTGATTCGGAGAGCTTCAAAGACGATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGCAGCCGGGAATGCTGTAAAGGATGTCAGCTTTTATGGTATAGCCGAAGGCATTGCAAGAGGAGGAGTTCCCTCAGCAAGAGTTGCTGCATATAGAGTCTGCGGTGACCAAGGATGCCCTACAGAGGCTATCTTGGCTGCTATTGCCGATGGAGTTGATATCATTACAATTTCAATAGGAAGCCCGGATGTAACTGTACTACTGCAGCATGATCCAATTGCTATCAGATCTTTCCATGCAATGGCAAACGGGATACTTACATCAAACTCGGCAGGCAATAATGGTCCTCGAGCTAGTACCATAACAAGTGTAGCACCATGTTACTTACAGTTGCAGCAAGTACTACAGATCGTAGAATCATTGACAAGAGTTGACTGGGATGACGATTCGCCCATTTTCGCCCTTCCTGCAACAACTTTGAGTGACAAAGAGTATAATGTGATCAAGTCTTATTACATGAACTCCACGAAAAATCCACGAGTAAACATACTAAAATGTGAAGCCGAAAGAGATGCTGCTGCACCTGTTGTTGCTTCCTTCTCATCACGTGGACCTAGTCAAATTTTACCAGAAATTATCAAGCCAGATATAAGTGCCCCAGGGGTAGCTATATCGGCTGCTTATTCACCTATTGCTTCAATCACAGACAACCCTGAAGACAAGAGGCATGTAAATTACAGTATACCATCCGGAACCTCCATGTCTTGCCCCCACGCCACTGCAGCCATCAAATCATCTCTTATGACTACAGCTTCTCCCATGAATTTTACCTACAATGATTATAACGCCGCTGCTGAATTTGCTTGTGGATGTGGACATATCAATCCTGTCAAAGCTATAGACCCGGGGCTTGTATATGAAGCTTCTAAGGAAGACTACATAAAGTTGCTCTGCTTGGTCTTAAACCAGACCAGTGTTAGACTTATATCAGGAGATAACAGCACTTGCTCTACAGGCTCTGACAAAGGATCTTTACATGATCACAATTACCCATCGCTGGCAGGTCCTGTCACACCAAAGAAACCTTTTTCATTCAAATTTCACAGAAAAGTTAAGAATGTTGGCCTTGCAAACTCCACCTACAAGGCCAAAATATTCGCCGGCAACTCTACTGATGAAGCTGACATCAAAGTGGTGCCTGAAGTCCTTTCCTTCAAGTGCTTGAATGAAGAGAAGACTTTGCTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

558

Amino Acids

60.38

Weight (kDa)

6.09

Isoelectric Point (pI)

36.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 107 5.7e-08 Peptidase inhibitor I9
Peptidase_S8 PF00082 178 - 404 1.4e-20 Subtilase family
fn3_6 PF17766 488 - 554 1.2e-09 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1266
Acc36I ACCTGC 1 cut(s) 1475
AccB1I GGYRCC 1 cut(s) 1618
AccB7I CCANNNNNTGG 2 cut(s) 477, 724
AccI GTMKAC 2 cut(s) 169, 1165
AccIII TCCGGA 1 cut(s) 1172
AciI CCGC 2 cut(s) 585, 1272
AclWI GGATC 2 cut(s) 689, 1459
AcsI RAATTY 5 cut(s) 420, 1044, 1246, 1280, 1520
AcuI CTGAAG 2 cut(s) 1158, 1644
AcvI CACGTG 1 cut(s) 1031
AfaI GTAC 5 cut(s) 113, 143, 681, 781, 825
AfiI CCNNNNNNNGG 5 cut(s) 259, 477, 607, 724, 1480
AjnI CCWGG 3 cut(s) 21, 114, 1081
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 159, 295
AlwI GGATC 2 cut(s) 689, 1459
AlwNI CAGNNNCTG 2 cut(s) 1442, 1490
Ama87I CYCGRG 2 cut(s) 771, 1327
Aor13HI TCCGGA 1 cut(s) 1172
AoxI GGCC 3 cut(s) 425, 1546, 1569
ApoI RAATTY 5 cut(s) 420, 1044, 1246, 1280, 1520
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AspS9I GGNCC 4 cut(s) 461, 767, 1034, 1487
AsuC2I CCSGG 5 cut(s) 254, 490, 668, 1328, 1329
AsuHPI GGTGA 4 cut(s) 138, 149, 599, 1101
AsuNHI GCTAGC 1 cut(s) 272
AvaI CYCGRG 2 cut(s) 771, 1327
AvaII GGWCC 4 cut(s) 461, 767, 1034, 1487
BaeGI GKGCMC 1 cut(s) 1081
BanI GGYRCC 1 cut(s) 1618
BanII GRGCYC 1 cut(s) 19
BauI CACGAG 1 cut(s) 960
BbrPI CACGTG 1 cut(s) 1031
BbsI GAAGAC 3 cut(s) 1146, 1362, 1664
Bbv12I GWGCWC 1 cut(s) 19
BbvCI CCTCAGC 1 cut(s) 556
BccI CCATC 7 cut(s) 89, 168, 446, 626, 1177, 1217, 1483
BcgI CGANNNNNNTGC 6 cut(s) 64, 98, 518, 552, 977, 1011
BciT130I CCWGG 3 cut(s) 23, 116, 1083
BciVI GTATCC 1 cut(s) 729
BclI TGATCA 2 cut(s) 924, 1462
BcnI CCSGG 5 cut(s) 254, 490, 668, 1328, 1329
BcoDI GTCTC 2 cut(s) 159, 295
BfaI CTAG 4 cut(s) 173, 273, 777, 1038
BfmI CTRYAG 7 cut(s) 603, 686, 827, 1203, 1230, 1320, 1434
BfuAI ACCTGC 1 cut(s) 1475
BfuI GTATCC 1 cut(s) 729
BglII AGATCT 2 cut(s) 332, 710
BmcAI AGTACT 1 cut(s) 825
Bme1390I CCNGG 8 cut(s) 23, 116, 254, 490, 668, 1083, 1328, 1329
Bme18I GGWCC 4 cut(s) 461, 767, 1034, 1487
BmeT110I CYCGRG 2 cut(s) 771, 1327
BmgT120I GGNCC 4 cut(s) 461, 767, 1034, 1487
BmiI GGNNCC 2 cut(s) 1177, 1620
BmrFI CCNGG 8 cut(s) 23, 116, 254, 490, 668, 1083, 1328, 1329
BmrI ACTGGG 1 cut(s) 868
BmsI GCATC 2 cut(s) 587, 985
BmtI GCTAGC 1 cut(s) 276
BmuI ACTGGG 1 cut(s) 868
BpiI GAAGAC 3 cut(s) 1146, 1362, 1664
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 179, 211
BpmI CTGGAG 1 cut(s) 5
Bpu10I CCTNAGC 1 cut(s) 556
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 5 cut(s) 254, 490, 668, 1328, 1329
Bsa29I ATCGAT 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 1031
BsaJI CCNNGG 6 cut(s) 115, 592, 1081, 1082, 1327, 1328
BsaWI WCCGGW 1 cut(s) 1172
BsaXI ACNNNNNCTCC 4 cut(s) 929, 959, 1222, 1252
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 477, 607, 724, 1480
Bse118I RCCGGY 1 cut(s) 1583
Bse1I ACTGG 2 cut(s) 863, 1395
Bse3DI GCAATG 2 cut(s) 534, 729
BseAI TCCGGA 1 cut(s) 1172
BseBI CCWGG 3 cut(s) 23, 116, 1083
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 6 cut(s) 115, 592, 1081, 1082, 1327, 1328
BseGI GGATG 6 cut(s) 511, 602, 676, 868, 1169, 1298
BseLI CCNNNNNNNGG 5 cut(s) 259, 477, 607, 724, 1480
BseMI GCAATG 2 cut(s) 534, 729
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 2 cut(s) 863, 1395
BseRI GAGGAG 3 cut(s) 456, 558, 561
BseSI GKGCMC 1 cut(s) 1081
BsgI GTGCAG 1 cut(s) 987
BshFI GGCC 3 cut(s) 427, 1548, 1571
BshNI GGYRCC 1 cut(s) 1618
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 19
BsiHKCI CYCGRG 2 cut(s) 771, 1327
BsiSI CCGG 6 cut(s) 253, 489, 668, 1173, 1328, 1584
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 5 cut(s) 259, 477, 607, 724, 1480
BsmAI GTCTC 2 cut(s) 159, 295
BsmFI GGGAC 1 cut(s) 353
BsmI GAATGC 1 cut(s) 499
BsnI GGCC 3 cut(s) 427, 1548, 1571
BsoBI CYCGRG 2 cut(s) 771, 1327
Bsp1286I GDGCHC 2 cut(s) 19, 1081
Bsp13I TCCGGA 1 cut(s) 1172
Bsp143I GATC 7 cut(s) 332, 694, 710, 831, 924, 1451, 1462
BspACI CCGC 2 cut(s) 585, 1272
BspANI GGCC 3 cut(s) 427, 1548, 1571
BspCNI CTCAG 1 cut(s) 569
BspDI ATCGAT 1 cut(s) 84
BspEI TCCGGA 1 cut(s) 1172
BspLI GGNNCC 2 cut(s) 1177, 1620
BspMAI CTGCAG 2 cut(s) 690, 1207
BspMI ACCTGC 1 cut(s) 1475
BspOI GCTAGC 1 cut(s) 276
BspPI GGATC 2 cut(s) 689, 1459
BspT107I GGYRCC 1 cut(s) 1618
BsrDI GCAATG 2 cut(s) 534, 729
BsrFI RCCGGY 1 cut(s) 1583
BsrI ACTGG 2 cut(s) 863, 1395
BssAI RCCGGY 1 cut(s) 1583
BssECI CCNNGG 6 cut(s) 115, 592, 1081, 1082, 1327, 1328
BssMI GATC 7 cut(s) 332, 694, 710, 831, 924, 1451, 1462
BssNAI GTATAC 2 cut(s) 170, 1166
BssSI CACGAG 1 cut(s) 960
BssT1I CCWWGG 1 cut(s) 592
Bst1107I GTATAC 2 cut(s) 170, 1166
Bst2BI CACGAG 1 cut(s) 960
Bst2UI CCWGG 3 cut(s) 23, 116, 1083
Bst4CI ACNGT 3 cut(s) 679, 813, 1163
Bst6I CTCTTC 1 cut(s) 1647
BstAPI GCANNNNNTGC 1 cut(s) 566
BstBAI YACGTR 1 cut(s) 1031
BstC8I GCNNGC 5 cut(s) 270, 274, 757, 1482, 1585
BstDEI CTNAG 2 cut(s) 556, 1350
BstEII GGTNACC 1 cut(s) 587
BstF5I GGATG 6 cut(s) 511, 602, 676, 868, 1169, 1298
BstKTI GATC 7 cut(s) 335, 697, 713, 834, 927, 1454, 1465
BstMAI GTCTC 2 cut(s) 159, 295
BstMBI GATC 7 cut(s) 332, 694, 710, 831, 924, 1451, 1462
BstMWI GCNNNNNNNGC 9 cut(s) 494, 566, 617, 626, 890, 1097, 1205, 1283, 1437
BstNI CCWGG 3 cut(s) 23, 116, 1083
BstNSI RCATGY 1 cut(s) 1154
BstPI GGTNACC 1 cut(s) 587
BstSCI CCNGG 8 cut(s) 21, 114, 252, 488, 666, 1081, 1326, 1327
BstSFI CTRYAG 7 cut(s) 603, 686, 827, 1203, 1230, 1320, 1434
BstSLI GKGCMC 1 cut(s) 1081
BstV2I GAAGAC 3 cut(s) 1146, 1362, 1664
BstX2I RGATCY 3 cut(s) 332, 710, 1451
BstYI RGATCY 3 cut(s) 332, 710, 1451
BstZ17I GTATAC 2 cut(s) 170, 1166
Bsu15I ATCGAT 1 cut(s) 84
BsuI GTATCC 1 cut(s) 729
BsuRI GGCC 3 cut(s) 427, 1548, 1571
BsuTUI ATCGAT 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 1461
BtsCI GGATG 6 cut(s) 511, 602, 676, 868, 1169, 1298
BtsI GCAGTG 1 cut(s) 1200
BtsIMutI CAGTG 3 cut(s) 246, 1200, 1402
BveI ACCTGC 1 cut(s) 1475
Cac8I GCNNGC 5 cut(s) 270, 274, 757, 1482, 1585
CaiI CAGNNNCTG 2 cut(s) 1442, 1490
Cfr10I RCCGGY 1 cut(s) 1583
Cfr13I GGNCC 4 cut(s) 461, 767, 1034, 1487
Cfr9I CCCGGG 1 cut(s) 1327
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 5 cut(s) 112, 142, 680, 780, 824
CviAII CATG 9 cut(s) 277, 692, 719, 801, 940, 1151, 1183, 1243, 1460
CviQI GTAC 5 cut(s) 112, 142, 680, 780, 824
DdeI CTNAG 2 cut(s) 556, 1350
DpnI GATC 7 cut(s) 334, 696, 712, 833, 926, 1453, 1464
DpnII GATC 7 cut(s) 332, 694, 710, 831, 924, 1451, 1462
Eam1104I CTCTTC 1 cut(s) 1647
EarI CTCTTC 1 cut(s) 1647
Ecl136II GAGCTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 592
Eco24I GRGCYC 1 cut(s) 19
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 4 cut(s) 461, 767, 1034, 1487
Eco53kI GAGCTC 1 cut(s) 17
Eco57I CTGAAG 2 cut(s) 1158, 1644
Eco72I CACGTG 1 cut(s) 1031
Eco88I CYCGRG 2 cut(s) 771, 1327
Eco91I GGTNACC 1 cut(s) 587
EcoICRI GAGCTC 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 1487
EcoO65I GGTNACC 1 cut(s) 587
EcoRII CCWGG 3 cut(s) 21, 114, 1081
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 592
FaeI CATG 9 cut(s) 280, 695, 722, 804, 943, 1154, 1186, 1246, 1463
FalI AAGNNNNNCTT 2 cut(s) 295, 327
FaqI GGGAC 1 cut(s) 353
FatI CATG 9 cut(s) 276, 691, 718, 800, 939, 1150, 1182, 1242, 1459
FbaI TGATCA 2 cut(s) 924, 1462
FblI GTMKAC 2 cut(s) 169, 1165
FokI GGATG 6 cut(s) 518, 609, 683, 875, 1156, 1305
FriOI GRGCYC 1 cut(s) 19
FspBI CTAG 4 cut(s) 173, 273, 777, 1038
GsuI CTGGAG 1 cut(s) 5
HaeIII GGCC 3 cut(s) 427, 1548, 1571
HapII CCGG 6 cut(s) 253, 489, 668, 1173, 1328, 1584
Hin1II CATG 9 cut(s) 280, 695, 722, 804, 943, 1154, 1186, 1246, 1463
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 1 cut(s) 1344
HinfI GANTC 5 cut(s) 413, 431, 579, 839, 869
HpaII CCGG 6 cut(s) 253, 489, 668, 1173, 1328, 1584
HphI GGTGA 4 cut(s) 138, 149, 599, 1101
Hpy166II GTNNAC 8 cut(s) 112, 142, 170, 856, 967, 1034, 1166, 1298
Hpy188I TCNGA 3 cut(s) 436, 710, 1444
Hpy188III TCNNGA 5 cut(s) 128, 187, 417, 1173, 1413
Hpy8I GTNNAC 8 cut(s) 112, 142, 170, 856, 967, 1034, 1166, 1298
HpyAV CCTTC 4 cut(s) 524, 896, 1030, 1645
HpyCH4III ACNGT 3 cut(s) 679, 813, 1163
HpyCH4IV ACGT 1 cut(s) 1030
HpyF10VI GCNNNNNNNGC 9 cut(s) 494, 566, 617, 626, 890, 1097, 1205, 1283, 1437
HpyF3I CTNAG 2 cut(s) 556, 1350
HpySE526I ACGT 1 cut(s) 1030
Hsp92II CATG 9 cut(s) 280, 695, 722, 804, 943, 1154, 1186, 1246, 1463
Kpn2I TCCGGA 1 cut(s) 1172
KroI GCCGGC 1 cut(s) 1583
KroNI GCCGGC 1 cut(s) 1585
Ksp22I TGATCA 2 cut(s) 924, 1462
Kzo9I GATC 7 cut(s) 332, 694, 710, 831, 924, 1451, 1462
LmnI GCTCC 1 cut(s) 105
LweI GCATC 2 cut(s) 587, 985
MaeI CTAG 4 cut(s) 173, 273, 777, 1038
MaeII ACGT 1 cut(s) 1030
MaeIII GTNAC 6 cut(s) 120, 587, 673, 803, 905, 1492
MalI GATC 7 cut(s) 334, 696, 712, 833, 926, 1453, 1464
MboI GATC 7 cut(s) 332, 694, 710, 831, 924, 1451, 1462
MboII GAAGA 6 cut(s) 58, 61, 1151, 1367, 1664, 1669
MfeI CAATTG 1 cut(s) 699
MflI RGATCY 3 cut(s) 332, 710, 1451
MhlI GDGCHC 2 cut(s) 19, 1081
MlyI GAGTC 2 cut(s) 407, 588
MmeI TCCRAC 2 cut(s) 340, 391
MroI TCCGGA 1 cut(s) 1172
MroNI GCCGGC 1 cut(s) 1583
MseI TTAA 2 cut(s) 1385, 1536
MslI CAYNNNNRTG 1 cut(s) 1613
MspA1I CMGCKG 1 cut(s) 1274
MspI CCGG 6 cut(s) 253, 489, 668, 1173, 1328, 1584
MspR9I CCNGG 8 cut(s) 23, 116, 254, 490, 668, 1083, 1328, 1329
MunI CAATTG 1 cut(s) 699
Mva1269I GAATGC 1 cut(s) 499
MvaI CCWGG 3 cut(s) 23, 116, 1083
MwoI GCNNNNNNNGC 9 cut(s) 494, 566, 617, 626, 890, 1097, 1205, 1283, 1437
NaeI GCCGGC 1 cut(s) 1585
NciI CCSGG 5 cut(s) 254, 490, 668, 1328, 1329
NdeII GATC 7 cut(s) 332, 694, 710, 831, 924, 1451, 1462
NgoMIV GCCGGC 1 cut(s) 1583
NheI GCTAGC 1 cut(s) 272
NlaIII CATG 9 cut(s) 280, 695, 722, 804, 943, 1154, 1186, 1246, 1463
NlaIV GGNNCC 2 cut(s) 1177, 1620
NmeAIII GCCGAG 1 cut(s) 731
NmuCI GTSAC 4 cut(s) 120, 587, 905, 1492
NspI RCATGY 1 cut(s) 1154
PaeR7I CTCGAG 1 cut(s) 771
PasI CCCWGGG 1 cut(s) 1082
PctI GAATGC 1 cut(s) 499
PdiI GCCGGC 1 cut(s) 1585
PfeI GAWTC 3 cut(s) 431, 839, 869
PflFI GACNNNGTC 1 cut(s) 1038
PflMI CCANNNNNTGG 2 cut(s) 477, 724
PleI GAGTC 2 cut(s) 407, 587
PmaCI CACGTG 1 cut(s) 1031
PmlI CACGTG 1 cut(s) 1031
PpsI GAGTC 2 cut(s) 407, 587
Ppu21I YACGTR 1 cut(s) 1031
PpuMI RGGWCCY 1 cut(s) 1487
PsiI TTATAA 1 cut(s) 1266
Psp124BI GAGCTC 1 cut(s) 19
Psp5II RGGWCCY 1 cut(s) 1487
Psp6I CCWGG 3 cut(s) 21, 114, 1081
PspCI CACGTG 1 cut(s) 1031
PspEI GGTNACC 1 cut(s) 587
PspGI CCWGG 3 cut(s) 21, 114, 1081
PspN4I GGNNCC 2 cut(s) 1177, 1620
PspPI GGNCC 4 cut(s) 461, 767, 1034, 1487
PspPPI RGGWCCY 1 cut(s) 1487
PspXI VCTCGAGB 1 cut(s) 771
PstI CTGCAG 2 cut(s) 690, 1207
PstNI CAGNNNCTG 2 cut(s) 1442, 1490
PsuI RGATCY 3 cut(s) 332, 710, 1451
PsyI GACNNNGTC 1 cut(s) 1038
RsaI GTAC 5 cut(s) 113, 143, 681, 781, 825
RsaNI GTAC 5 cut(s) 112, 142, 680, 780, 824
RseI CAYNNNNRTG 1 cut(s) 1613
SacI GAGCTC 1 cut(s) 19
SaqAI TTAA 2 cut(s) 1385, 1536
Sau3AI GATC 7 cut(s) 332, 694, 710, 831, 924, 1451, 1462
Sau96I GGNCC 4 cut(s) 461, 767, 1034, 1487
ScaI AGTACT 1 cut(s) 825
SchI GAGTC 2 cut(s) 407, 588
ScrFI CCNGG 8 cut(s) 23, 116, 254, 490, 668, 1083, 1328, 1329
SduI GDGCHC 2 cut(s) 19, 1081
SfaNI GCATC 2 cut(s) 587, 985
SfcI CTRYAG 7 cut(s) 603, 686, 827, 1203, 1230, 1320, 1434
Sfr274I CTCGAG 1 cut(s) 771
SinI GGWCC 4 cut(s) 461, 767, 1034, 1487
SlaI CTCGAG 1 cut(s) 771
SmaI CCCGGG 1 cut(s) 1329
SmiMI CAYNNNNRTG 1 cut(s) 1613
SmlI CTYRAG 2 cut(s) 58, 771
SmoI CTYRAG 2 cut(s) 58, 771
SsiI CCGC 2 cut(s) 585, 1272
SspI AATATT 1 cut(s) 1578
SspMI CTAG 4 cut(s) 173, 273, 777, 1038
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 8 cut(s) 21, 114, 252, 488, 666, 1081, 1326, 1327
StyI CCWWGG 1 cut(s) 592
TaaI ACNGT 3 cut(s) 679, 813, 1163
TaiI ACGT 1 cut(s) 1033
TaqI TCGA 3 cut(s) 84, 186, 772
TatI WGTACW 3 cut(s) 141, 679, 823
TauI GCSGC 1 cut(s) 1274
TfiI GAWTC 3 cut(s) 431, 839, 869
Tru1I TTAA 2 cut(s) 1385, 1536
Tru9I TTAA 2 cut(s) 1385, 1536
TscAI CASTG 3 cut(s) 253, 1207, 1402
TseFI GTSAC 4 cut(s) 120, 587, 905, 1492
Tsp45I GTSAC 4 cut(s) 120, 587, 905, 1492
TspDTI ATGAA 8 cut(s) 102, 293, 956, 1259, 1356, 1503, 1614, 1665
TspMI CCCGGG 1 cut(s) 1327
TspRI CASTG 3 cut(s) 253, 1207, 1402
Tth111I GACNNNGTC 1 cut(s) 1038
Van91I CCANNNNNTGG 2 cut(s) 477, 724
VpaK11BI GGWCC 4 cut(s) 461, 767, 1034, 1487
XapI RAATTY 5 cut(s) 420, 1044, 1246, 1280, 1520
XceI RCATGY 1 cut(s) 1154
XhoI CTCGAG 1 cut(s) 771
XmaI CCCGGG 1 cut(s) 1327
XmiI GTMKAC 2 cut(s) 169, 1165
XspI CTAG 4 cut(s) 173, 273, 777, 1038
ZrmI AGTACT 1 cut(s) 825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.