Rmu_sc0002263.1_g000074

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002263.1
OG0000257
Physical Location & Seq
Reverse (-)
283616 .. 285829
2214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002263.1_g000074.1.cds

Sequence Viewer

Length: 2214 bp
atggccaagcatggagcattcctattctcttatgctttctctactctcatacttactatgagcttattgtgcaaggccattgatcatgaagataaaaaggttcatatagtgtacttggggtcacttcctgatgatgaggagtactcgccaagttcccatcacctcgatatactacaaagagttgttgatggcatttctgctgaaaattgcttgataaggagttaccaaaggagcttcaatggatttgctgcaaagctcagtgatcatgaaaaagaaaagattgctaacatgggggaggtgatctccgtctttcctagcagaacgttccaacttcaaacgacgaggtcatgggactttatgggtctcaagctgacaagtcctcgaaaagtcactgctgagagtaatgttattgttggtgtgattgacactggaattgaccctgattcggagagctttagagatgaaggttttggtgctgctcctatgaagtggaaaggtgtttgtgaaggtggagaaaatttcagttgcaacaagaagataattggagctcgtttttacggagcagcagagtctgcaagggatgaagacggccatggaacccacactgcctcaacggcagcagggaatgaggtaaggggtgtgagcttttacggattagaaccaggtacagcaagaggaggggttccctcagcgagaattgctgcgtataaagtctgtggggctacagggtgctcttcagaggctatattggctgcttttgacgatgcaattgctgatggagttgacattgtgtcagtctcacttggaagtgcaagtccccttccttttgataatgatcctatagcaattggttcttatcatgcaatgacgagagggatattgacatcaaactctgcaggcaacagtggtcctggagctggtactgtaacaagtgtagcgccgtggatgcttgcagttgcagcaagtagcaaagatcgtcggatcattgacaaggccgttcttggaagtaggacactagtaggggcttcagtgaactctttcatattgaaaggtagttttccactgatatatggaaaagatgcttcaagtaactgccccgaatactatgctaggaattgtgcagatggttgcctagacagtaattcagttcggaacaagatcgtgctatgtgaccggcactctggattttatgaggctgatcgagctggtgcacgaggcgtaattttctacaatcccatacctgatgatgcttctcttgtcccaatgcctgctgtgggtttgagatccgaagactataatggggccatatcttacatgaactcgacaaaagatcctcaagcaagcatatcaaagagtgaaaacataaaagatccagctgcacctgaagttgtttctttctcttcgcgtggacctaatcaaattttacctgaaatcattaagccagatgtaactggcccgggagtgaccattatagctgcatatccgagtgatgttcctgtcacaagtggctctagagacacaaggcgggtcaagtacaatatactatccggaacctcaatgtcttgcccccacgctactggtgtagctgcatatgtaaaagagttccaccctgattggtctccatctgccattaaatctgctcttatgactacagcttggcctatgaatcatacaaccaacagctcatgcccgggtgaatttgcatatggatccggtcatatcaatcctctaaaagctattgacccagggcttgtgtatgatgcttctgcagaggattatattaagttactctgcttggacatggacgaggcgagagtcagacttatatcaggagataacagcacttgtcctacagcttctgaacaaggatcggcaaaggatctcaactacccttcaatatcagctattgttacaccaatgacacgttttacggtgacaatacgtagaagagttacaaatgttggccttgcaaattccacttacaaggccgaaatcatgccaaactctcaagttgaaatcaaagtggagcctcaagttctttccttcaagtccgtgaacgaggagaagacttttgatttgaccattgttggaagagggttgccagatggatcacatatgtcttcgtccttggtttggtccgacggaactcataaggttaaaagtccagttctagtgcaaagcttatcagcagcagcatcatcacggtttcattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

737

Amino Acids

78.64

Weight (kDa)

5.97

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1405
AccIII TCCGGA 1 cut(s) 1547
AciI CCGC 1 cut(s) 1525
AclI AACGTT 1 cut(s) 323
AcoI YGGCCR 2 cut(s) 3, 589
AcsI RAATTY 4 cut(s) 517, 1419, 1697, 1972
AcuI CTGAAG 3 cut(s) 720, 1011, 1404
AfaI GTAC 5 cut(s) 113, 143, 667, 922, 1535
AfiI CCNNNNNNNGG 4 cut(s) 445, 917, 1274, 2133
AflIII ACRYGT 1 cut(s) 1922
AgsI TTSAA 7 cut(s) 238, 335, 1048, 1086, 1896, 2015, 2047
AhdI GACNNNNNGTC 1 cut(s) 790
AhlI ACTAGT 1 cut(s) 1015
AjnI CCWGG 3 cut(s) 661, 910, 1744
Alw21I GWGCWC 3 cut(s) 550, 734, 1213
Alw26I GTCTC 4 cut(s) 368, 802, 1509, 1623
Alw44I GTGCAC 1 cut(s) 1209
AlwNI CAGNNNCTG 2 cut(s) 572, 1859
Ama87I CYCGRG 2 cut(s) 1456, 1690
Aor13HI TCCGGA 1 cut(s) 1547
AoxI GGCC 9 cut(s) 3, 75, 589, 993, 1302, 1453, 1658, 1963, 1986
ApaLI GTGCAC 1 cut(s) 1209
ApoI RAATTY 4 cut(s) 517, 1419, 1697, 1972
ArsI GACNNNNNNTTYG 2 cut(s) 2059, 2091
Asp700I GAANNNNTTC 1 cut(s) 1037
AspLEI GCGC 1 cut(s) 940
AspS9I GGNCC 5 cut(s) 908, 1302, 1409, 1454, 2136
AsuC2I CCSGG 4 cut(s) 1457, 1458, 1691, 1692
AsuHPI GGTGA 4 cut(s) 152, 310, 1706, 1945
AvaI CYCGRG 2 cut(s) 1456, 1690
AvaII GGWCC 3 cut(s) 908, 1409, 2136
BaeGI GKGCMC 1 cut(s) 1213
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1709
BanII GRGCYC 1 cut(s) 550
BauI CACGAG 1 cut(s) 1212
BbsI GAAGAC 4 cut(s) 591, 1296, 2072, 2112
Bbv12I GWGCWC 3 cut(s) 550, 734, 1213
BbvCI CCTCAGC 1 cut(s) 688
BccI CCATC 6 cut(s) 165, 182, 770, 1118, 1630, 2099
BceAI ACGGC 4 cut(s) 604, 630, 925, 980
BcgI CGANNNNNNTGC 4 cut(s) 752, 786, 1088, 1122
BciT130I CCWGG 3 cut(s) 663, 912, 1746
BclI TGATCA 2 cut(s) 82, 262
BcnI CCSGG 4 cut(s) 1457, 1458, 1691, 1692
BcoDI GTCTC 4 cut(s) 368, 802, 1509, 1623
BcuI ACTAGT 1 cut(s) 1015
BfaI CTAG 6 cut(s) 315, 1016, 1110, 1133, 1512, 2171
BfmI CTRYAG 6 cut(s) 723, 840, 894, 1650, 1767, 1851
BfoI RGCGCY 1 cut(s) 941
BglI GCCNNNNNGGC 1 cut(s) 614
BmcAI AGTACT 1 cut(s) 143
Bme1390I CCNGG 7 cut(s) 663, 912, 1457, 1458, 1691, 1692, 1746
Bme18I GGWCC 3 cut(s) 908, 1409, 2136
BmeRI GACNNNNNGTC 1 cut(s) 790
BmeT110I CYCGRG 2 cut(s) 1456, 1690
BmgT120I GGNCC 5 cut(s) 908, 1302, 1409, 1454, 2136
BmiI GGNNCC 6 cut(s) 598, 684, 1303, 1552, 1711, 2028
BmrFI CCNGG 7 cut(s) 663, 912, 1457, 1458, 1691, 1692, 1746
BmsI GCATC 6 cut(s) 754, 936, 1069, 1237, 1750, 2204
BpiI GAAGAC 4 cut(s) 591, 1296, 2072, 2112
BplI GAGNNNNNCTC 4 cut(s) 128, 160, 287, 319
BpmI CTGGAG 1 cut(s) 933
Bpu10I CCTNAGC 1 cut(s) 688
BpuEI CTTGAG 4 cut(s) 350, 1320, 1992, 2016
BpuMI CCSGG 4 cut(s) 1457, 1458, 1691, 1692
BsaAI YACGTR 1 cut(s) 1943
BsaBI GATNNNNATC 1 cut(s) 834
BsaI GGTCTC 2 cut(s) 368, 1623
BsaJI CCNNGG 7 cut(s) 592, 941, 1456, 1690, 1744, 1745, 2127
BsaWI WCCGGW 2 cut(s) 1547, 1712
Bsc4I CCNNNNNNNGG 4 cut(s) 445, 917, 1274, 2133
Bse118I RCCGGY 1 cut(s) 1173
Bse1I ACTGG 4 cut(s) 433, 1456, 1582, 2165
Bse3DI GCAATG 1 cut(s) 870
Bse8I GATNNNNATC 1 cut(s) 834
BseAI TCCGGA 1 cut(s) 1547
BseBI CCWGG 3 cut(s) 663, 912, 1746
BseDI CCNNGG 7 cut(s) 592, 941, 1456, 1690, 1744, 1745, 2127
BseGI GGATG 2 cut(s) 586, 951
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 4 cut(s) 445, 917, 1274, 2133
BseMI GCAATG 1 cut(s) 870
BseMII CTCAG 3 cut(s) 271, 387, 702
BseNI ACTGG 4 cut(s) 433, 1456, 1582, 2165
BseRI GAGGAG 3 cut(s) 152, 690, 2075
BseSI GKGCMC 1 cut(s) 1213
BsgI GTGCAG 2 cut(s) 1140, 1362
Bsh1236I CGCG 1 cut(s) 1405
BshFI GGCC 9 cut(s) 5, 77, 591, 995, 1304, 1455, 1660, 1965, 1988
BsiHKAI GWGCWC 3 cut(s) 550, 734, 1213
BsiHKCI CYCGRG 2 cut(s) 1456, 1690
BsiSI CCGG 5 cut(s) 1174, 1457, 1548, 1691, 1713
BslFI GGGAC 3 cut(s) 365, 801, 1244
BslI CCNNNNNNNGG 4 cut(s) 445, 917, 1274, 2133
BsmAI GTCTC 4 cut(s) 368, 802, 1509, 1623
BsmFI GGGAC 3 cut(s) 365, 801, 1244
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 9 cut(s) 5, 77, 591, 995, 1304, 1455, 1660, 1965, 1988
Bso31I GGTCTC 2 cut(s) 368, 1623
BsoBI CYCGRG 2 cut(s) 1456, 1690
Bsp1286I GDGCHC 3 cut(s) 550, 734, 1213
Bsp13I TCCGGA 1 cut(s) 1547
Bsp19I CCATGG 1 cut(s) 592
BspACI CCGC 1 cut(s) 1525
BspANI GGCC 9 cut(s) 5, 77, 591, 995, 1304, 1455, 1660, 1965, 1988
BspCNI CTCAG 3 cut(s) 270, 388, 701
BspEI TCCGGA 1 cut(s) 1547
BspFNI CGCG 1 cut(s) 1405
BspHI TCATGA 2 cut(s) 85, 265
BspLI GGNNCC 6 cut(s) 598, 684, 1303, 1552, 1711, 2028
BspMAI CTGCAG 2 cut(s) 898, 1771
BspQI GCTCTTC 1 cut(s) 739
BspTNI GGTCTC 2 cut(s) 368, 1623
BsrDI GCAATG 1 cut(s) 870
BsrFI RCCGGY 1 cut(s) 1173
BsrI ACTGG 4 cut(s) 433, 1456, 1582, 2165
BssAI RCCGGY 1 cut(s) 1173
BssECI CCNNGG 7 cut(s) 592, 941, 1456, 1690, 1744, 1745, 2127
BssSI CACGAG 1 cut(s) 1212
BssT1I CCWWGG 2 cut(s) 592, 2127
Bst2BI CACGAG 1 cut(s) 1212
Bst2UI CCWGG 3 cut(s) 663, 912, 1746
Bst4CI ACNGT 5 cut(s) 905, 925, 1139, 1933, 2205
Bst6I CTCTTC 4 cut(s) 739, 1405, 1942, 2086
BstAPI GCANNNNNTGC 1 cut(s) 572
BstBAI YACGTR 1 cut(s) 1943
BstC8I GCNNGC 4 cut(s) 898, 951, 1269, 1342
BstDEI CTNAG 3 cut(s) 257, 396, 688
BstDSI CCRYGG 2 cut(s) 592, 941
BstF5I GGATG 2 cut(s) 586, 951
BstFNI CGCG 1 cut(s) 1405
BstH2I RGCGCY 1 cut(s) 941
BstHHI GCGC 1 cut(s) 940
BstMAI GTCTC 4 cut(s) 368, 802, 1509, 1623
BstMWI GCNNNNNNNGC 8 cut(s) 69, 572, 614, 698, 749, 946, 959, 1202
BstNI CCWGG 3 cut(s) 663, 912, 1746
BstSCI CCNGG 7 cut(s) 661, 910, 1455, 1456, 1689, 1690, 1744
BstSFI CTRYAG 6 cut(s) 723, 840, 894, 1650, 1767, 1851
BstSLI GKGCMC 1 cut(s) 1213
BstSNI TACGTA 1 cut(s) 1943
BstUI CGCG 1 cut(s) 1405
BstV2I GAAGAC 4 cut(s) 591, 1296, 2072, 2112
BstX2I RGATCY 5 cut(s) 1283, 1330, 1369, 1709, 1879
BstXI CCANNNNNNTGG 1 cut(s) 1577
BstYI RGATCY 5 cut(s) 1283, 1330, 1369, 1709, 1879
BsuRI GGCC 9 cut(s) 5, 77, 591, 995, 1304, 1455, 1660, 1965, 1988
BtgI CCRYGG 2 cut(s) 592, 941
BtsCI GGATG 2 cut(s) 586, 951
BtsI GCAGTG 2 cut(s) 390, 603
BtsIMutI CAGTG 7 cut(s) 265, 390, 426, 603, 910, 1035, 1061
Cac8I GCNNGC 4 cut(s) 898, 951, 1269, 1342
CaiI CAGNNNCTG 2 cut(s) 572, 1859
CciI TCATGA 2 cut(s) 85, 265
CfoI GCGC 1 cut(s) 940
Cfr10I RCCGGY 1 cut(s) 1173
Cfr13I GGNCC 5 cut(s) 908, 1302, 1409, 1454, 2136
Cfr9I CCCGGG 2 cut(s) 1456, 1690
CsiI ACCWGGT 1 cut(s) 661
Csp6I GTAC 5 cut(s) 112, 142, 666, 921, 1534
CspCI CAANNNNNGTGG 2 cut(s) 1595, 1630
CviQI GTAC 5 cut(s) 112, 142, 666, 921, 1534
DdeI CTNAG 3 cut(s) 257, 396, 688
DriI GACNNNNNGTC 1 cut(s) 790
EaeI YGGCCR 2 cut(s) 3, 589
Eam1104I CTCTTC 4 cut(s) 739, 1405, 1942, 2086
Eam1105I GACNNNNNGTC 1 cut(s) 790
EarI CTCTTC 4 cut(s) 739, 1405, 1942, 2086
Ecl136II GAGCTC 1 cut(s) 548
Eco105I TACGTA 1 cut(s) 1943
Eco130I CCWWGG 2 cut(s) 592, 2127
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 2 cut(s) 368, 1623
Eco47I GGWCC 3 cut(s) 908, 1409, 2136
Eco53kI GAGCTC 1 cut(s) 548
Eco57I CTGAAG 3 cut(s) 720, 1011, 1404
Eco88I CYCGRG 2 cut(s) 1456, 1690
EcoICRI GAGCTC 1 cut(s) 548
EcoRII CCWGG 3 cut(s) 661, 910, 1744
EcoT14I CCWWGG 2 cut(s) 592, 2127
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 2 cut(s) 592, 2127
FaqI GGGAC 3 cut(s) 365, 801, 1244
FauI CCCGC 1 cut(s) 1518
FauNDI CATATG 3 cut(s) 1591, 1705, 2115
FbaI TGATCA 2 cut(s) 82, 262
FokI GGATG 2 cut(s) 593, 958
FriOI GRGCYC 1 cut(s) 550
FspBI CTAG 6 cut(s) 315, 1016, 1110, 1133, 1512, 2171
GlaI GCGC 1 cut(s) 939
GsuI CTGGAG 1 cut(s) 933
HaeII RGCGCY 1 cut(s) 941
HaeIII GGCC 9 cut(s) 5, 77, 591, 995, 1304, 1455, 1660, 1965, 1988
HapII CCGG 5 cut(s) 1174, 1457, 1548, 1691, 1713
HhaI GCGC 1 cut(s) 940
Hin6I GCGC 1 cut(s) 938
HinP1I GCGC 1 cut(s) 938
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HindIII AAGCTT 1 cut(s) 2179
HinfI GANTC 4 cut(s) 443, 569, 1666, 1815
HpaII CCGG 5 cut(s) 1174, 1457, 1548, 1691, 1713
HphI GGTGA 4 cut(s) 152, 310, 1706, 1945
Hpy166II GTNNAC 6 cut(s) 112, 784, 1033, 1211, 1409, 2056
Hpy188I TCNGA 9 cut(s) 448, 739, 981, 1152, 1288, 1485, 1820, 1861, 2140
Hpy188III TCNNGA 7 cut(s) 86, 128, 266, 1182, 1512, 1548, 1830
Hpy8I GTNNAC 6 cut(s) 112, 784, 1033, 1211, 1409, 2056
Hpy99I CGWCG 3 cut(s) 343, 981, 2144
HpyAV CCTTC 5 cut(s) 458, 500, 830, 1902, 2053
HpyCH4III ACNGT 5 cut(s) 905, 925, 1139, 1933, 2205
HpyCH4IV ACGT 3 cut(s) 323, 1924, 1942
HpyF10VI GCNNNNNNNGC 8 cut(s) 69, 572, 614, 698, 749, 946, 959, 1202
HpyF3I CTNAG 3 cut(s) 257, 396, 688
HpySE526I ACGT 3 cut(s) 323, 1924, 1942
HspAI GCGC 1 cut(s) 938
Kpn2I TCCGGA 1 cut(s) 1547
Ksp22I TGATCA 2 cut(s) 82, 262
LguI GCTCTTC 1 cut(s) 739
LmnI GCTCC 7 cut(s) 14, 231, 484, 545, 560, 914, 2026
LweI GCATC 6 cut(s) 754, 936, 1069, 1237, 1750, 2204
MabI ACCWGGT 1 cut(s) 661
MaeI CTAG 6 cut(s) 315, 1016, 1110, 1133, 1512, 2171
MaeII ACGT 3 cut(s) 323, 1924, 1942
MfeI CAATTG 2 cut(s) 768, 846
MflI RGATCY 5 cut(s) 1283, 1330, 1369, 1709, 1879
MhlI GDGCHC 3 cut(s) 550, 734, 1213
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 578, 1824
MmeI TCCRAC 4 cut(s) 352, 959, 2068, 2163
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 1547
MroXI GAANNNNTTC 1 cut(s) 1037
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 1437, 1632, 1782, 2157, 2212
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1376
MspI CCGG 5 cut(s) 1174, 1457, 1548, 1691, 1713
MspR9I CCNGG 7 cut(s) 663, 912, 1457, 1458, 1691, 1692, 1746
MunI CAATTG 2 cut(s) 768, 846
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 3 cut(s) 663, 912, 1746
MvnI CGCG 1 cut(s) 1405
MwoI GCNNNNNNNGC 8 cut(s) 69, 572, 614, 698, 749, 946, 959, 1202
NciI CCSGG 4 cut(s) 1457, 1458, 1691, 1692
NcoI CCATGG 1 cut(s) 592
NdeI CATATG 3 cut(s) 1591, 1705, 2115
NlaIV GGNNCC 6 cut(s) 598, 684, 1303, 1552, 1711, 2028
NmuCI GTSAC 6 cut(s) 120, 388, 1169, 1462, 1498, 1933
PagI TCATGA 2 cut(s) 85, 265
PasI CCCWGGG 1 cut(s) 1745
PciSI GCTCTTC 1 cut(s) 739
PctI GAATGC 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 2 cut(s) 443, 1666
PflFI GACNNNGTC 1 cut(s) 343
PfoI TCCNGGA 1 cut(s) 910
PleI GAGTC 2 cut(s) 577, 1823
PpsI GAGTC 2 cut(s) 577, 1823
Ppu21I YACGTR 1 cut(s) 1943
Psp124BI GAGCTC 1 cut(s) 550
Psp1406I AACGTT 1 cut(s) 323
Psp6I CCWGG 3 cut(s) 661, 910, 1744
PspGI CCWGG 3 cut(s) 661, 910, 1744
PspN4I GGNNCC 6 cut(s) 598, 684, 1303, 1552, 1711, 2028
PspPI GGNCC 5 cut(s) 908, 1302, 1409, 1454, 2136
PstI CTGCAG 2 cut(s) 898, 1771
PstNI CAGNNNCTG 2 cut(s) 572, 1859
PsuI RGATCY 5 cut(s) 1283, 1330, 1369, 1709, 1879
PsyI GACNNNGTC 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 1376
RsaI GTAC 5 cut(s) 113, 143, 667, 922, 1535
RsaNI GTAC 5 cut(s) 112, 142, 666, 921, 1534
SacI GAGCTC 1 cut(s) 550
SapI GCTCTTC 1 cut(s) 739
SaqAI TTAA 5 cut(s) 1437, 1632, 1782, 2157, 2212
Sau96I GGNCC 5 cut(s) 908, 1302, 1409, 1454, 2136
ScaI AGTACT 1 cut(s) 143
SchI GAGTC 2 cut(s) 578, 1824
ScrFI CCNGG 7 cut(s) 663, 912, 1457, 1458, 1691, 1692, 1746
SduI GDGCHC 3 cut(s) 550, 734, 1213
SexAI ACCWGGT 1 cut(s) 661
SfaNI GCATC 6 cut(s) 754, 936, 1069, 1237, 1750, 2204
SfcI CTRYAG 6 cut(s) 723, 840, 894, 1650, 1767, 1851
SinI GGWCC 3 cut(s) 908, 1409, 2136
SmaI CCCGGG 2 cut(s) 1458, 1692
SmlI CTYRAG 4 cut(s) 365, 1335, 2007, 2031
SmoI CTYRAG 4 cut(s) 365, 1335, 2007, 2031
SnaBI TACGTA 1 cut(s) 1943
SpeI ACTAGT 1 cut(s) 1015
SsiI CCGC 1 cut(s) 1525
SspMI CTAG 6 cut(s) 315, 1016, 1110, 1133, 1512, 2171
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 7 cut(s) 661, 910, 1455, 1456, 1689, 1690, 1744
StyI CCWWGG 2 cut(s) 592, 2127
TaaI ACNGT 5 cut(s) 905, 925, 1139, 1933, 2205
TaiI ACGT 3 cut(s) 326, 1927, 1945
TaqI TCGA 4 cut(s) 165, 382, 1201, 1322
TatI WGTACW 3 cut(s) 111, 141, 1533
TfiI GAWTC 2 cut(s) 443, 1666
Tru1I TTAA 5 cut(s) 1437, 1632, 1782, 2157, 2212
Tru9I TTAA 5 cut(s) 1437, 1632, 1782, 2157, 2212
TscAI CASTG 7 cut(s) 265, 397, 433, 610, 910, 1035, 1068
TseFI GTSAC 6 cut(s) 120, 388, 1169, 1462, 1498, 1933
Tsp45I GTSAC 6 cut(s) 120, 388, 1169, 1462, 1498, 1933
TspGWI ACGGA 5 cut(s) 295, 573, 666, 2041, 2157
TspMI CCCGGG 2 cut(s) 1456, 1690
TspRI CASTG 7 cut(s) 265, 397, 433, 610, 910, 1035, 1068
Tth111I GACNNNGTC 1 cut(s) 343
VneI GTGCAC 1 cut(s) 1209
VpaK11BI GGWCC 3 cut(s) 908, 1409, 2136
XapI RAATTY 4 cut(s) 517, 1419, 1697, 1972
XbaI TCTAGA 1 cut(s) 1511
XmaI CCCGGG 2 cut(s) 1456, 1690
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 6 cut(s) 315, 1016, 1110, 1133, 1512, 2171
ZrmI AGTACT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.