Rorug01G0037800

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000257
Physical Location & Seq
Forward (+)
6230436 .. 6238766
8331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0037800.1

Sequence Viewer

Length: 2226 bp
ATGAACCCTGAAAGTCTGATAGCTTCGGCGGCCATAAACGTAGGCCTGGCCATCGTGATTCTCTCACTCTTCTCCATCCTGAAGAAGCAACCCTCGTACGCCCCTATCTACTACCCTCGCCGCCTCTCTCAGTCGCAGTCATCGCAGCCGTCGCAGCGCCACCGCCAAAACATCCCGGATTTCGATGAAGCCTTCACTGTCCGCCGCTTCCTCCCTTCCCTCTCCTGGATTCGCCGCGCCTACCGCGTCTCCGAGGATGAGATCCTCGAGACCAGTGGCCTCGACGCCCTCATCATCATCAGGCTCTTCAAGTTTGGTATCAAATTCTTTTTGGTTTGCTCTCTTGTGGGATTGGCAGTACTTGTTCCGGTAAACTACTGTGGCCAGGGTCATCTCATAGCCCACCAGAGTCATCTAGCTGTGAGCCACCATTCGTTGGATCTCTTCAGTATATCTAACATCAGTCCAGGTTCTAACAGGCTTTGGGTTCATTGTTCATGCTTATGGTTTATATCTCTGTATGGAGTATATCTACTGTACAAGGAATATAATGAGATTTTGTCTAAAAGGATTCAACAACTTCGTAACATTAGGCATCAGCCTGATCAGTTCACTGTTCTAGTCCGGGAAATTCCCTTCTGCAATGATCACAGATCTCGCGGATGTTGTGTTGATAAATTCTTCTCTAAGCATCATCCATATGCTTATCATTCTTATCAGATTTTGTACGATGGAAAAGAATTTCAGGAGTTGCTGGCAAAATCCATTGAAAGAAAGATAGAGGACTTGAGCAAGCGGTCCATGGGAAAGAAACAAAACGAGACCATGGGAAATAAGAAACATAATGAGACGCCTTTGCTCTCTAATCATTCTCATCAAGATGCTGCTAAATTAGCTTCACATGAGGAAAAGCTTGAAGAACTTCATCATAAGATACACCAGTTACAGTCTGAAAATATGCTTAAAGAAAAGGAGTTACCTGTTGCTTTTGTTACATTCAAGTCTCGGTTGGGTGCAGCACTAGTTTCCCAATCTCAGCAGCATGCAGATCCACTTCTTTGGATAACTGACATGGCTCCAGAACCGAGGGATGTATCTTGGAGAAATCTGGTGATACCATACAGAGTTTTGCCACTTTGCAGGCTAGGTGTCATTGTTGCAGCCTCTCTTCTTACAATTTTCTTTGCAATCCCAGTCACTGCTGTCCAGGGTATAGCCAAGTTTGAGAAACTAAAAAAATGGTTTCCTCCAGCCATGGCTTTACAGTTGATACCAGGGTTAAGCTCTATCATAACAGGATATCTTCCAAGTGCTGTTCTGAAAGGGTTCATATACATTGTTCCTTTTGCAATGCTTGGAATGGCTAAACTAGCTGGCTGTATATCCAAAAGCAAGGAGGAAATAAAAGCTTCCAACATGGTTTTTTATTTTTTGGTTGGAAATGTGTTCTTTTGGAGCTTGTTATCTGGGTCTTTACTAGATGAAATTGGGCAATCTTTTACTCATCCGAAAGATATTCCTGGTCACCTTGCTATTGCTGTTTCTGCTCAAGCAGACTTCTTTGTGACATATATTTTGACAGATGGACTGTCAGGGTTTTCTTTGGAGATCCTTCAACCAGGTTTACTTTGTTGGGATGCTATAAAGTCTTGTACATATGGCCGGGGGAAAGAGAAAACTCCTTATCTTTACTCATTACCTTACTACAGAATCATTCCTTCAGTCTCACTGTCTGTGCTGATTGGCATGGTATATGCGGTTGTCACACCACTTCTGCTCCCATTTCTGATTGGCTACTTCTGTCTGGGCTATGTTGTATATATAAATCAGATTGAAGATGTGTATGAAACAGTTTATGACACATGTGGACAGTATTGGCCATACATCCACCATTATATTCTCATTGCACTCGTTCTTATGCAAATTACAATGATTGGTCTTTTCGGTCTGAAATCAAAGCCCGCTGCATCCATTTCCACAATTCCACTTCTCTTGCTGACATTGATGTTTAATGAATATTGCAAGATTCATTTTCTTCCTACATTCCGCCTTTTATCCATTCAGAATGCCAAGGAATTTGATGAACTTGATGTGAAGTGCAATCAGATGGAGTTTAACCATGAGAGTGCCAGCATTGCTTATTGCCCACCTTGTTTGCGTCCCTTGAACGTGATGGAATCAGAGCCAAGCTATTCACCTTCCATAGTTTCTACATCATCGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

741

Amino Acids

83.96

Weight (kDa)

8.56

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 6 - 182 4.7e-39 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 203 - 369 9.4e-24 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 382 - 648 1.6e-74 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 436
AccII CGCG 3 cut(s) 237, 246, 660
AclWI GGATC 4 cut(s) 256, 447, 1043, 1603
AcoI YGGCCR 5 cut(s) 30, 48, 382, 1660, 1877
AcsI RAATTY 5 cut(s) 323, 630, 677, 740, 2075
AcuI CTGAAG 3 cut(s) 101, 430, 1704
AcyI GRCGYC 2 cut(s) 285, 851
AfaI GTAC 5 cut(s) 98, 360, 539, 728, 1654
AfiI CCNNNNNNNGG 2 cut(s) 225, 436
AflIII ACRYGT 1 cut(s) 1862
AgsI TTSAA 8 cut(s) 310, 575, 770, 917, 1000, 1616, 1835, 2167
AhlI ACTAGT 1 cut(s) 1021
AjnI CCWGG 8 cut(s) 45, 224, 384, 466, 1206, 1273, 1519, 1618
AluBI AGCT 9 cut(s) 23, 419, 896, 913, 1284, 1373, 1409, 1458, 2190
AluI AGCT 9 cut(s) 23, 419, 896, 913, 1284, 1373, 1409, 1458, 2190
Alw26I GTCTC 6 cut(s) 253, 263, 815, 842, 1008, 1729
AlwI GGATC 4 cut(s) 256, 447, 1043, 1603
AlwNI CAGNNNCTG 1 cut(s) 1199
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 7 cut(s) 30, 43, 48, 277, 382, 1660, 1877
ApeKI GCWGC 7 cut(s) 145, 154, 884, 1016, 1039, 1160, 1964
ApoI RAATTY 5 cut(s) 323, 630, 677, 740, 2075
ArsI GACNNNNNNTTYG 2 cut(s) 1558, 1590
Asp700I GAANNNNTTC 2 cut(s) 921, 1325
AspLEI GCGC 2 cut(s) 159, 239
AspS9I GGNCC 1 cut(s) 798
AsuC2I CCSGG 3 cut(s) 176, 626, 1664
AsuHPI GGTGA 3 cut(s) 1123, 1517, 2187
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 798
BaeI ACNNNNGTAYC 4 cut(s) 926, 959, 1262, 1295
BalI TGGCCA 3 cut(s) 50, 384, 1879
BbvI GCAGC 7 cut(s) 157, 166, 871, 1028, 1051, 1172, 1951
BccI CCATC 6 cut(s) 59, 83, 725, 1577, 2101, 2167
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 8 cut(s) 47, 226, 386, 468, 1208, 1275, 1521, 1620
BclI TGATCA 2 cut(s) 604, 646
BcnI CCSGG 3 cut(s) 176, 626, 1664
BcoDI GTCTC 6 cut(s) 253, 263, 815, 842, 1008, 1729
BcuI ACTAGT 1 cut(s) 1021
BfaI CTAG 6 cut(s) 416, 620, 1022, 1145, 1370, 1478
BfmI CTRYAG 1 cut(s) 1705
BfoI RGCGCY 1 cut(s) 160
BglII AGATCT 1 cut(s) 653
BmcAI AGTACT 1 cut(s) 360
Bme18I GGWCC 1 cut(s) 798
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 1 cut(s) 798
BmiI GGNNCC 1 cut(s) 1077
BmrI ACTGGG 1 cut(s) 1187
BmsI GCATC 5 cut(s) 604, 700, 871, 1627, 1976
BmuI ACTGGG 1 cut(s) 1187
BpmI CTGGAG 2 cut(s) 1062, 1233
BpuEI CTTGAG 2 cut(s) 808, 1533
BpuMI CCSGG 3 cut(s) 176, 626, 1664
Bsa29I ATCGAT 1 cut(s) 2219
BsaHI GRCGYC 2 cut(s) 285, 851
BsaI GGTCTC 2 cut(s) 263, 815
BsaWI WCCGGW 1 cut(s) 367
BsaXI ACNNNNNCTCC 4 cut(s) 233, 263, 1761, 1791
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 436
Bse1I ACTGG 3 cut(s) 273, 940, 1193
Bse3DI GCAATG 4 cut(s) 649, 1356, 1902, 2133
BseBI CCWGG 8 cut(s) 47, 226, 386, 468, 1208, 1275, 1521, 1620
BseCI ATCGAT 1 cut(s) 2219
BseLI CCNNNNNNNGG 2 cut(s) 225, 436
BseMI GCAATG 4 cut(s) 649, 1356, 1902, 2133
BseMII CTCAG 2 cut(s) 143, 1049
BseNI ACTGG 3 cut(s) 273, 940, 1193
BseXI GCAGC 7 cut(s) 157, 166, 871, 1028, 1051, 1172, 1951
BsgI GTGCAG 1 cut(s) 1035
Bsh1236I CGCG 3 cut(s) 237, 246, 660
BshFI GGCC 7 cut(s) 32, 45, 50, 279, 384, 1662, 1879
BshVI ATCGAT 1 cut(s) 2219
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 4 cut(s) 176, 368, 625, 1663
BsiWI CGTACG 1 cut(s) 96
BslFI GGGAC 1 cut(s) 2145
BslI CCNNNNNNNGG 2 cut(s) 225, 436
BsmAI GTCTC 6 cut(s) 253, 263, 815, 842, 1008, 1729
BsmBI CGTCTC 2 cut(s) 253, 842
BsmFI GGGAC 1 cut(s) 2145
BsmI GAATGC 1 cut(s) 2071
BsnI GGCC 7 cut(s) 32, 45, 50, 279, 384, 1662, 1879
Bso31I GGTCTC 2 cut(s) 263, 815
BsoBI CYCGRG 1 cut(s) 266
Bsp1407I TGTACA 2 cut(s) 537, 1652
Bsp143I GATC 7 cut(s) 261, 439, 604, 646, 653, 1048, 1608
Bsp19I CCATGG 3 cut(s) 801, 825, 1254
BspANI GGCC 7 cut(s) 32, 45, 50, 279, 384, 1662, 1879
BspCNI CTCAG 2 cut(s) 142, 1048
BspDI ATCGAT 1 cut(s) 2219
BspFNI CGCG 3 cut(s) 237, 246, 660
BspLI GGNNCC 1 cut(s) 1077
BspPI GGATC 4 cut(s) 256, 447, 1043, 1603
BspQI GCTCTTC 1 cut(s) 311
BspTNI GGTCTC 2 cut(s) 263, 815
BsrDI GCAATG 4 cut(s) 649, 1356, 1902, 2133
BsrGI TGTACA 2 cut(s) 537, 1652
BsrI ACTGG 3 cut(s) 273, 940, 1193
BssMI GATC 7 cut(s) 261, 439, 604, 646, 653, 1048, 1608
BssNI GRCGYC 2 cut(s) 285, 851
BssT1I CCWWGG 4 cut(s) 801, 825, 1254, 2070
Bst2UI CCWGG 8 cut(s) 47, 226, 386, 468, 1208, 1275, 1521, 1620
Bst6I CTCTTC 4 cut(s) 74, 311, 449, 1173
BstACI GRCGYC 2 cut(s) 285, 851
BstAUI TGTACA 2 cut(s) 537, 1652
BstC8I GCNNGC 7 cut(s) 756, 794, 1044, 1142, 1375, 1962, 2131
BstDEI CTNAG 3 cut(s) 129, 687, 1035
BstDSI CCRYGG 3 cut(s) 801, 825, 1254
BstEII GGTNACC 1 cut(s) 1523
BstFNI CGCG 3 cut(s) 237, 246, 660
BstH2I RGCGCY 1 cut(s) 160
BstHHI GCGC 2 cut(s) 159, 239
BstKTI GATC 7 cut(s) 264, 442, 607, 649, 656, 1051, 1611
BstMAI GTCTC 6 cut(s) 253, 263, 815, 842, 1008, 1729
BstMBI GATC 7 cut(s) 261, 439, 604, 646, 653, 1048, 1608
BstMWI GCNNNNNNNGC 9 cut(s) 29, 142, 151, 154, 243, 893, 1370, 1544, 2135
BstNI CCWGG 8 cut(s) 47, 226, 386, 468, 1208, 1275, 1521, 1620
BstNSI RCATGY 2 cut(s) 1046, 1866
BstPI GGTNACC 1 cut(s) 1523
BstSFI CTRYAG 1 cut(s) 1705
BstUI CGCG 3 cut(s) 237, 246, 660
BstV1I GCAGC 7 cut(s) 157, 166, 871, 1028, 1051, 1172, 1951
BstX2I RGATCY 5 cut(s) 261, 439, 653, 1048, 1608
BstXI CCANNNNNNTGG 1 cut(s) 1059
BstYI RGATCY 5 cut(s) 261, 439, 653, 1048, 1608
Bsu15I ATCGAT 1 cut(s) 2219
BsuRI GGCC 7 cut(s) 32, 45, 50, 279, 384, 1662, 1879
BsuTUI ATCGAT 1 cut(s) 2219
BtgI CCRYGG 3 cut(s) 801, 825, 1254
BtgZI GCGATG 1 cut(s) 126
BtsI GCAGTG 1 cut(s) 1197
BtsIMutI CAGTG 5 cut(s) 195, 280, 612, 1197, 1727
Cac8I GCNNGC 7 cut(s) 756, 794, 1044, 1142, 1375, 1962, 2131
CaiI CAGNNNCTG 1 cut(s) 1199
CfoI GCGC 2 cut(s) 159, 239
Cfr13I GGNCC 1 cut(s) 798
ClaI ATCGAT 1 cut(s) 2219
CseI GACGC 4 cut(s) 235, 293, 859, 2147
CsiI ACCWGGT 1 cut(s) 1618
Csp6I GTAC 5 cut(s) 97, 359, 538, 727, 1653
CspCI CAANNNNNGTGG 4 cut(s) 1974, 2009, 2136, 2171
CviQI GTAC 5 cut(s) 97, 359, 538, 727, 1653
DdeI CTNAG 3 cut(s) 129, 687, 1035
DpnI GATC 7 cut(s) 263, 441, 606, 648, 655, 1050, 1610
DpnII GATC 7 cut(s) 261, 439, 604, 646, 653, 1048, 1608
EaeI YGGCCR 5 cut(s) 30, 48, 382, 1660, 1877
Eam1104I CTCTTC 4 cut(s) 74, 311, 449, 1173
EarI CTCTTC 4 cut(s) 74, 311, 449, 1173
EciI GGCGGA 2 cut(s) 191, 2036
Eco130I CCWWGG 4 cut(s) 801, 825, 1254, 2070
Eco147I AGGCCT 1 cut(s) 45
Eco31I GGTCTC 2 cut(s) 263, 815
Eco32I GATATC 1 cut(s) 1301
Eco47I GGWCC 1 cut(s) 798
Eco57I CTGAAG 3 cut(s) 101, 430, 1704
Eco88I CYCGRG 1 cut(s) 266
Eco91I GGTNACC 1 cut(s) 1523
EcoO65I GGTNACC 1 cut(s) 1523
EcoRII CCWGG 8 cut(s) 45, 224, 384, 466, 1206, 1273, 1519, 1618
EcoRV GATATC 1 cut(s) 1301
EcoT14I CCWWGG 4 cut(s) 801, 825, 1254, 2070
ErhI CCWWGG 4 cut(s) 801, 825, 1254, 2070
Esp3I CGTCTC 2 cut(s) 253, 842
FaqI GGGAC 1 cut(s) 2145
FauI CCCGC 1 cut(s) 1969
FauNDI CATATG 2 cut(s) 700, 1657
FbaI TGATCA 2 cut(s) 604, 646
FspBI CTAG 6 cut(s) 416, 620, 1022, 1145, 1370, 1478
GlaI GCGC 2 cut(s) 158, 238
GsuI CTGGAG 2 cut(s) 1062, 1233
HaeII RGCGCY 1 cut(s) 160
HaeIII GGCC 7 cut(s) 32, 45, 50, 279, 384, 1662, 1879
HapII CCGG 4 cut(s) 176, 368, 625, 1663
HgaI GACGC 4 cut(s) 235, 293, 859, 2147
HhaI GCGC 2 cut(s) 159, 239
Hin1I GRCGYC 2 cut(s) 285, 851
Hin6I GCGC 2 cut(s) 157, 237
HinP1I GCGC 2 cut(s) 157, 237
HindIII AAGCTT 2 cut(s) 911, 1407
HinfI GANTC 7 cut(s) 58, 229, 409, 571, 1710, 2026, 2177
HpaII CCGG 4 cut(s) 176, 368, 625, 1663
HphI GGTGA 3 cut(s) 1123, 1517, 2187
Hpy166II GTNNAC 4 cut(s) 373, 612, 1625, 1868
Hpy188III TCNNGA 6 cut(s) 55, 79, 268, 746, 878, 1079
Hpy8I GTNNAC 4 cut(s) 373, 612, 1625, 1868
Hpy99I CGWCG 2 cut(s) 154, 287
HpyAV CCTTC 6 cut(s) 202, 225, 646, 1622, 1728, 2208
HpyCH4IV ACGT 2 cut(s) 39, 2169
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 142, 151, 154, 243, 893, 1370, 1544, 2135
HpyF3I CTNAG 3 cut(s) 129, 687, 1035
HpySE526I ACGT 2 cut(s) 39, 2169
Hsp92I GRCGYC 2 cut(s) 285, 851
HspAI GCGC 2 cut(s) 157, 237
Ksp22I TGATCA 2 cut(s) 604, 646
Kzo9I GATC 7 cut(s) 261, 439, 604, 646, 653, 1048, 1608
LguI GCTCTTC 1 cut(s) 311
LmnI GCTCC 3 cut(s) 1081, 1455, 1782
Lsp1109I GCAGC 7 cut(s) 157, 166, 871, 1028, 1051, 1172, 1951
LweI GCATC 5 cut(s) 604, 700, 871, 1627, 1976
MabI ACCWGGT 1 cut(s) 1618
MaeI CTAG 6 cut(s) 416, 620, 1022, 1145, 1370, 1478
MaeII ACGT 2 cut(s) 39, 2169
MaeIII GTNAC 8 cut(s) 584, 942, 975, 991, 1195, 1523, 1564, 1762
MalI GATC 7 cut(s) 263, 441, 606, 648, 655, 1050, 1610
MboI GATC 7 cut(s) 261, 439, 604, 646, 653, 1048, 1608
MflI RGATCY 5 cut(s) 261, 439, 653, 1048, 1608
MlsI TGGCCA 3 cut(s) 50, 384, 1879
MluNI TGGCCA 3 cut(s) 50, 384, 1879
MlyI GAGTC 1 cut(s) 418
MmeI TCCRAC 3 cut(s) 417, 1417, 1437
Mox20I TGGCCA 3 cut(s) 50, 384, 1879
MroXI GAANNNNTTC 2 cut(s) 921, 1325
MscI TGGCCA 3 cut(s) 50, 384, 1879
MseI TTAA 5 cut(s) 963, 1280, 2010, 2115, 2224
MslI CAYNNNNRTG 4 cut(s) 502, 699, 879, 2124
Msp20I TGGCCA 3 cut(s) 50, 384, 1879
MspA1I CMGCKG 1 cut(s) 1964
MspI CCGG 4 cut(s) 176, 368, 625, 1663
Mva1269I GAATGC 1 cut(s) 2071
MvaI CCWGG 8 cut(s) 47, 226, 386, 468, 1208, 1275, 1521, 1620
MvnI CGCG 3 cut(s) 237, 246, 660
MwoI GCNNNNNNNGC 9 cut(s) 29, 142, 151, 154, 243, 893, 1370, 1544, 2135
NciI CCSGG 3 cut(s) 176, 626, 1664
NcoI CCATGG 3 cut(s) 801, 825, 1254
NdeI CATATG 2 cut(s) 700, 1657
NdeII GATC 7 cut(s) 261, 439, 604, 646, 653, 1048, 1608
NlaIV GGNNCC 1 cut(s) 1077
NmuCI GTSAC 4 cut(s) 1195, 1523, 1564, 1762
NspI RCATGY 2 cut(s) 1046, 1866
PaeI GCATGC 1 cut(s) 1046
PaeR7I CTCGAG 1 cut(s) 266
PceI AGGCCT 1 cut(s) 45
PciI ACATGT 1 cut(s) 1862
PciSI GCTCTTC 1 cut(s) 311
PctI GAATGC 1 cut(s) 2071
PdmI GAANNNNTTC 2 cut(s) 921, 1325
PfeI GAWTC 6 cut(s) 58, 229, 571, 1710, 2026, 2177
Pfl23II CGTACG 1 cut(s) 96
PflMI CCANNNNNTGG 1 cut(s) 436
PfoI TCCNGGA 3 cut(s) 174, 224, 624
PleI GAGTC 1 cut(s) 417
PpsI GAGTC 1 cut(s) 417
PscI ACATGT 1 cut(s) 1862
Psp6I CCWGG 8 cut(s) 45, 224, 384, 466, 1206, 1273, 1519, 1618
PspEI GGTNACC 1 cut(s) 1523
PspGI CCWGG 8 cut(s) 45, 224, 384, 466, 1206, 1273, 1519, 1618
PspLI CGTACG 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 1077
PspPI GGNCC 1 cut(s) 798
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 5 cut(s) 261, 439, 653, 1048, 1608
RsaI GTAC 5 cut(s) 98, 360, 539, 728, 1654
RsaNI GTAC 5 cut(s) 97, 359, 538, 727, 1653
RseI CAYNNNNRTG 4 cut(s) 502, 699, 879, 2124
SapI GCTCTTC 1 cut(s) 311
SaqAI TTAA 5 cut(s) 963, 1280, 2010, 2115, 2224
Sau3AI GATC 7 cut(s) 261, 439, 604, 646, 653, 1048, 1608
Sau96I GGNCC 1 cut(s) 798
ScaI AGTACT 1 cut(s) 360
SchI GAGTC 1 cut(s) 418
SexAI ACCWGGT 1 cut(s) 1618
SfaNI GCATC 5 cut(s) 604, 700, 871, 1627, 1976
SfcI CTRYAG 1 cut(s) 1705
Sfr274I CTCGAG 1 cut(s) 266
SinI GGWCC 1 cut(s) 798
SlaI CTCGAG 1 cut(s) 266
SmiMI CAYNNNNRTG 4 cut(s) 502, 699, 879, 2124
SmlI CTYRAG 3 cut(s) 266, 787, 1548
SmoI CTYRAG 3 cut(s) 266, 787, 1548
SpeI ACTAGT 1 cut(s) 1021
SphI GCATGC 1 cut(s) 1046
SseBI AGGCCT 1 cut(s) 45
SspI AATATT 1 cut(s) 2018
SspMI CTAG 6 cut(s) 416, 620, 1022, 1145, 1370, 1478
StuI AGGCCT 1 cut(s) 45
StyI CCWWGG 4 cut(s) 801, 825, 1254, 2070
TaiI ACGT 2 cut(s) 42, 2172
TaqI TCGA 4 cut(s) 183, 267, 282, 2219
TaqII GACCGA 1 cut(s) 1934
TatI WGTACW 3 cut(s) 358, 537, 1652
TauI GCSGC 4 cut(s) 32, 123, 207, 237
TfiI GAWTC 6 cut(s) 58, 229, 571, 1710, 2026, 2177
Tru1I TTAA 5 cut(s) 963, 1280, 2010, 2115, 2224
Tru9I TTAA 5 cut(s) 963, 1280, 2010, 2115, 2224
TscAI CASTG 5 cut(s) 202, 280, 619, 1204, 1734
TseFI GTSAC 4 cut(s) 1195, 1523, 1564, 1762
TseI GCWGC 7 cut(s) 145, 154, 884, 1016, 1039, 1160, 1964
Tsp45I GTSAC 4 cut(s) 1195, 1523, 1564, 1762
TspRI CASTG 5 cut(s) 202, 280, 619, 1204, 1734
Van91I CCANNNNNTGG 1 cut(s) 436
VpaK11BI GGWCC 1 cut(s) 798
XapI RAATTY 5 cut(s) 323, 630, 677, 740, 2075
XceI RCATGY 2 cut(s) 1046, 1866
XhoI CTCGAG 1 cut(s) 266
XmnI GAANNNNTTC 2 cut(s) 921, 1325
XspI CTAG 6 cut(s) 416, 620, 1022, 1145, 1370, 1478
ZrmI AGTACT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.