Rh1BG047500

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000257
Physical Location & Seq
Reverse (-)
6536831 .. 6561868
25038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG047500.1

Sequence Viewer

Length: 2358 bp
ATGCCAAAGATGCATCGTAAGTTAAAGGAAATAAAATCTGGATGCATAATGGAGATGTGGTACTACGTTATTAATCTCAGTATAAACTATAAAGCACTCTTCACTAACAACATTTTAGAGCATCGATCAAAAACAATGGCTAAGCATGGAGCTATCCTATTTTCGTATGCTTTCCCCACTCTTATACTAACCTTGAGCTTTTTCCTGTGCGAAGCCATTGATGAAAACAGAACGATCCACATTGTGTACCTAGGTTCACTTCCTGATAATGAGGTGTACTCACCAACGTCTCACCACCTTGATTTACTCCAAAAAGTTGTCGACAGCAACTCCGCTGCAAATTTCTTGACAAGAAGTTACAAAAGGAGTTTCAGTGGATTTGCTGCCAAGCTCACTGACCGGGAAAGGGAAAGGCTTGCTAACATGAAGGAAGTGGTCTCTGTCTTTCCGAGCAGAATTCTCCATCCTCAGACAACAAGATCTTGGGACTTTATCGGTTTCAATGAGAAAATAAGACGGAATGCCAGTGTTGAGAGTGATACCATTATTGGTGTCATTGACTCTGGAATTTGGCCTGAATCGGAGAGTTTTAAAGATGATGGTTTTGGTCCTCCTCCCAAGAAATGGAAAGGTGCTTGTGAGGGCGGCCAAAATTTCACTTGCAATAAGAAGCTAATTGGAGCCCGGGTTTACAGTTCATCTTCAGAGTCTGCAAGGGATGAACAAGGCCATGGAACCCACACAGCCTCAACTGCTGCAGGGAACGCTGTAAAGGATGTGAGCTTTTATGGACTAGCAGGAGGTACTGCAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGTGTATAAAGTATGTGATGATCATGGGTGCCCTAGTGATGCTATATTGGCTGCTTTTGATGATGCTATTGCGGATGGAGCTGACATCATTACAGTTTCATTAGGAAGCCAGGAAGCATCTTTATTACAGTATGATCCTATTGCTATCGGTGCTTTCCATGCAATGGCAAAGGGGGTACTTACATCAAATTCCGCAGGCAACAGTGGTCCTGATGATTCAACTGTTTCAAGTGTAGCACCATGGATACTTACAGTTGCAGCAAGTAGTACTGATCGTAGGATCATTGACAAGGTTGTTCTCGGAAATGGGACAACCATGGTTGGGGCTTCAGTGAACACTTTCAAATTAAATGGGACAAGTTTTCCATTGATCTATGGAAAAGATGCTTCAAGTAATTGCTCCGAGATCGAAGCTGGGAGCTGTTCAGACGGCTGCTTAGACAGTGGTTTAGTTAAGGGAAAAGTTGTGTTATGTGACGTGACTAACGGAGTTGATGAGGCCTATGTTTCTGGAGCAGTAGGCTTAATTTTGAGAGTTGATTATGATGATGTTTCTGAAATTGTACCCTTTCCTACAACAGCTCTTGCCAACAAAGAGCATAGTCTGATAAAGTTGTACACAAACTCCACGAAAGATCCTCGAGTAAACATACAAAGAAGTGAAGAAGAAAGGGATACTGCCGCACCTGTTGTTGCTTCATTCTCTTCACGTGGACCAAATTCAATTCTACCAGAAATTATCAAGCCAGATATAAGCGCCCCAGGGGTTACTATTTTGGCTGCCTTTTCACCTATTGCTTCAGTTACAGAGAGTCTTCAAGACACAAGGCGTGTAAAATATAGTATACTATCTGGAACCTCCATGTCTTGTCCCCACGCAGCTGGTGCAGCTGCTTATGTTAAAGCATTCCATCCTGATTGGTCTCCAGCAGCCATCAAATCATCTCTTATGACTACAGCTTCTCCCATGAATGTTACTGACAATAGCAGTGCCGCTGGTGAATTTGCTTATGGATCTGGACATATCAATCCTGTCAAAGCTATAGACCCAGGGCTTGTGTATGAAGCTTCTAAGGATGACTACGTAAAGCTGCTATGCTCGGTCTTGGATCAGGCCAATGTTAGACTTATATCAGGAGATAACAGCACTTGTCCTACAGCTTCTGAACAAGGATCGGCAAAGGATCTCAACTACCCTTCAATATCAGCTGTTGTTACACCAATGACACGTTTTATGGTGAAAATACGTAGAAGAGTTACAAATGTTGGCCTTGCAAATTCCACTTACAAGGCCGAAATCATGCCAAACTCTCAAGTTGACATCAAAGTGGAGCCTCAAGTTCTTTGCTTCAAGTCCGTAAACGAGGAGAAGACTTTTGATTTGACCATTGTTGGAAGAGGGTTGCCAGATGGATCACATATGTCTTCGTCCTTGGTTTGGTCCGACGAAACTCATAAGGTTAAAAGTCCAGTTCTAGTGCAAAGCTTATCAGCAGCAGCATCATCACGGTTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

785

Amino Acids

84.25

Weight (kDa)

6.25

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 80 - 155 6.3e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 179 - 620 2.2e-45 Subtilase family
fn3_6 PF17766 675 - 773 5e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 867
AccB7I CCANNNNNTGG 2 cut(s) 624, 1003
AccI GTMKAC 2 cut(s) 321, 1684
AciI CCGC 6 cut(s) 333, 645, 911, 1032, 1521, 1833
AclWI GGATC 9 cut(s) 229, 968, 1127, 1469, 1861, 1956, 2020, 2031, 2260
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 8 cut(s) 340, 456, 567, 652, 1027, 1558, 1841, 2116
AcuI CTGAAG 3 cut(s) 687, 1152, 1623
AcvI CACGTG 1 cut(s) 1550
AdeI CACNNNGTG 1 cut(s) 244
AfaI GTAC 8 cut(s) 62, 248, 278, 805, 1017, 1108, 1404, 1457
AfiI CCNNNNNNNGG 5 cut(s) 406, 624, 1003, 1161, 2277
AflIII ACRYGT 1 cut(s) 2066
AgsI TTSAA 9 cut(s) 502, 1059, 1068, 1183, 1230, 1563, 1658, 2040, 2191
AjiI CACGTC 1 cut(s) 1318
AjnI CCWGG 3 cut(s) 948, 1600, 1888
Alw26I GTCTC 3 cut(s) 294, 442, 1767
AlwI GGATC 9 cut(s) 229, 968, 1127, 1469, 1861, 1956, 2020, 2031, 2260
AlwNI CAGNNNCTG 2 cut(s) 710, 2003
Ama87I CYCGRG 2 cut(s) 684, 1479
AoxI GGCC 7 cut(s) 572, 646, 727, 1338, 1953, 2107, 2130
ApoI RAATTY 8 cut(s) 340, 456, 567, 652, 1027, 1558, 1841, 2116
ArsI GACNNNNNNTTYG 2 cut(s) 2203, 2235
AseI ATTAAT 1 cut(s) 72
Asp700I GAANNNNTTC 1 cut(s) 1178
AspA2I CCTAGG 1 cut(s) 250
AspLEI GCGC 1 cut(s) 1598
AspS9I GGNCC 4 cut(s) 608, 1046, 1553, 2280
AsuC2I CCSGG 3 cut(s) 401, 685, 686
AsuHPI GGTGA 5 cut(s) 273, 284, 1620, 1850, 2089
AvaI CYCGRG 2 cut(s) 684, 1479
AvaII GGWCC 4 cut(s) 608, 1046, 1553, 2280
AvrII CCTAGG 1 cut(s) 250
BaeGI GKGCMC 1 cut(s) 872
BanI GGYRCC 1 cut(s) 867
BanII GRGCYC 1 cut(s) 685
BbrPI CACGTG 1 cut(s) 1550
BbsI GAAGAC 3 cut(s) 1646, 2216, 2256
BbvCI CCTCAGC 1 cut(s) 826
BccI CCATC 6 cut(s) 471, 593, 908, 1758, 1781, 2243
BceAI ACGGC 1 cut(s) 1285
BciT130I CCWGG 3 cut(s) 950, 1602, 1890
BciVI GTATCC 2 cut(s) 1077, 1507
BclI TGATCA 1 cut(s) 859
BcnI CCSGG 3 cut(s) 401, 685, 686
BcoDI GTCTC 3 cut(s) 294, 442, 1767
BfaI CTAG 4 cut(s) 251, 794, 873, 2315
BfmI CTRYAG 4 cut(s) 756, 1794, 1881, 1995
BfoI RGCGCY 1 cut(s) 1599
BfuI GTATCC 2 cut(s) 1077, 1507
BglII AGATCT 1 cut(s) 479
BlnI CCTAGG 1 cut(s) 250
BlpI GCTNAGC 1 cut(s) 141
BmcAI AGTACT 1 cut(s) 1108
Bme1390I CCNGG 6 cut(s) 401, 685, 686, 950, 1602, 1890
Bme18I GGWCC 4 cut(s) 608, 1046, 1553, 2280
BmeT110I CYCGRG 2 cut(s) 684, 1479
BmgBI CACGTC 1 cut(s) 1318
BmgT120I GGNCC 4 cut(s) 608, 1046, 1553, 2280
BmiI GGNNCC 6 cut(s) 682, 736, 822, 869, 1696, 2172
BmrFI CCNGG 6 cut(s) 401, 685, 686, 950, 1602, 1890
BmsI GCATC 8 cut(s) 22, 32, 130, 868, 892, 965, 1213, 2348
BpiI GAAGAC 3 cut(s) 1646, 2216, 2256
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 2 cut(s) 1371, 1749
Bpu10I CCTNAGC 1 cut(s) 826
Bpu1102I GCTNAGC 1 cut(s) 141
BpuEI CTTGAG 3 cut(s) 214, 2136, 2160
BpuMI CCSGG 3 cut(s) 401, 685, 686
Bsa29I ATCGAT 1 cut(s) 124
BsaAI YACGTR 3 cut(s) 1550, 1924, 2087
BsaI GGTCTC 2 cut(s) 442, 1767
Bsc4I CCNNNNNNNGG 5 cut(s) 406, 624, 1003, 1161, 2277
Bse1I ACTGG 2 cut(s) 525, 2309
Bse3DI GCAATG 1 cut(s) 1008
BseBI CCWGG 3 cut(s) 950, 1602, 1890
BseCI ATCGAT 1 cut(s) 124
BseGI GGATG 7 cut(s) 47, 463, 724, 781, 919, 1750, 1921
BseLI CCNNNNNNNGG 5 cut(s) 406, 624, 1003, 1161, 2277
BseMI GCAATG 1 cut(s) 1008
BseMII CTCAG 3 cut(s) 91, 482, 840
BseNI ACTGG 2 cut(s) 525, 2309
BseRI GAGGAG 3 cut(s) 603, 828, 2219
BseSI GKGCMC 1 cut(s) 872
BseYI CCCAGC 1 cut(s) 1253
BsgI GTGCAG 1 cut(s) 1746
BshFI GGCC 7 cut(s) 574, 648, 729, 1340, 1955, 2109, 2132
BshNI GGYRCC 1 cut(s) 867
BshVI ATCGAT 1 cut(s) 124
BsiHKCI CYCGRG 2 cut(s) 684, 1479
BsiSI CCGG 2 cut(s) 400, 685
BslFI GGGAC 4 cut(s) 500, 1162, 1207, 1695
BslI CCNNNNNNNGG 5 cut(s) 406, 624, 1003, 1161, 2277
BsmAI GTCTC 3 cut(s) 294, 442, 1767
BsmBI CGTCTC 1 cut(s) 294
BsmFI GGGAC 4 cut(s) 500, 1162, 1207, 1695
BsmI GAATGC 2 cut(s) 526, 1745
BsnI GGCC 7 cut(s) 574, 648, 729, 1340, 1955, 2109, 2132
Bso31I GGTCTC 2 cut(s) 442, 1767
BsoBI CYCGRG 2 cut(s) 684, 1479
Bsp1286I GDGCHC 2 cut(s) 685, 872
Bsp1407I TGTACA 1 cut(s) 1455
Bsp1720I GCTNAGC 1 cut(s) 141
Bsp19I CCATGG 3 cut(s) 730, 1079, 1155
BspACI CCGC 6 cut(s) 333, 645, 911, 1032, 1521, 1833
BspANI GGCC 7 cut(s) 574, 648, 729, 1340, 1955, 2109, 2132
BspCNI CTCAG 3 cut(s) 90, 481, 839
BspDI ATCGAT 1 cut(s) 124
BspLI GGNNCC 6 cut(s) 682, 736, 822, 869, 1696, 2172
BspMAI CTGCAG 1 cut(s) 760
BspPI GGATC 9 cut(s) 229, 968, 1127, 1469, 1861, 1956, 2020, 2031, 2260
BspT107I GGYRCC 1 cut(s) 867
BspTNI GGTCTC 2 cut(s) 442, 1767
BsrDI GCAATG 1 cut(s) 1008
BsrGI TGTACA 1 cut(s) 1455
BsrI ACTGG 2 cut(s) 525, 2309
BssNAI GTATAC 1 cut(s) 1685
BssT1I CCWWGG 5 cut(s) 250, 730, 1079, 1155, 2271
Bst1107I GTATAC 1 cut(s) 1685
Bst2UI CCWGG 3 cut(s) 950, 1602, 1890
Bst4CI ACNGT 8 cut(s) 695, 934, 969, 1043, 1063, 1093, 1283, 2349
Bst6I CTCTTC 4 cut(s) 104, 1549, 2086, 2230
BstAPI GCANNNNNTGC 1 cut(s) 1724
BstAUI TGTACA 1 cut(s) 1455
BstBAI YACGTR 3 cut(s) 1550, 1924, 2087
BstC8I GCNNGC 2 cut(s) 417, 1036
BstDEI CTNAG 6 cut(s) 77, 141, 468, 826, 1276, 1911
BstDSI CCRYGG 3 cut(s) 730, 1079, 1155
BstF5I GGATG 7 cut(s) 47, 463, 724, 781, 919, 1750, 1921
BstH2I RGCGCY 1 cut(s) 1599
BstHHI GCGC 1 cut(s) 1598
BstMAI GTCTC 3 cut(s) 294, 442, 1767
BstNI CCWGG 3 cut(s) 950, 1602, 1890
BstSCI CCNGG 6 cut(s) 399, 683, 684, 948, 1600, 1888
BstSFI CTRYAG 4 cut(s) 756, 1794, 1881, 1995
BstSLI GKGCMC 1 cut(s) 872
BstSNI TACGTA 2 cut(s) 1924, 2087
BstV2I GAAGAC 3 cut(s) 1646, 2216, 2256
BstX2I RGATCY 4 cut(s) 479, 1474, 1853, 2023
BstXI CCANNNNNNTGG 1 cut(s) 1721
BstYI RGATCY 4 cut(s) 479, 1474, 1853, 2023
BstZ17I GTATAC 1 cut(s) 1685
Bsu15I ATCGAT 1 cut(s) 124
BsuI GTATCC 2 cut(s) 1077, 1507
BsuRI GGCC 7 cut(s) 574, 648, 729, 1340, 1955, 2109, 2132
BsuTUI ATCGAT 1 cut(s) 124
BtgI CCRYGG 3 cut(s) 730, 1079, 1155
BtrI CACGTC 1 cut(s) 1318
BtsCI GGATG 7 cut(s) 47, 463, 724, 781, 919, 1750, 1921
BtsI GCAGTG 1 cut(s) 1834
BtsIMutI CAGTG 7 cut(s) 379, 393, 532, 1048, 1176, 1288, 1834
Cac8I GCNNGC 2 cut(s) 417, 1036
CaiI CAGNNNCTG 2 cut(s) 710, 2003
CfoI GCGC 1 cut(s) 1598
Cfr13I GGNCC 4 cut(s) 608, 1046, 1553, 2280
Cfr9I CCCGGG 1 cut(s) 684
ClaI ATCGAT 1 cut(s) 124
Csp6I GTAC 8 cut(s) 61, 247, 277, 804, 1016, 1107, 1403, 1456
CviQI GTAC 8 cut(s) 61, 247, 277, 804, 1016, 1107, 1403, 1456
DdeI CTNAG 6 cut(s) 77, 141, 468, 826, 1276, 1911
DraI TTTAAA 1 cut(s) 592
DraIII CACNNNGTG 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 4 cut(s) 104, 1549, 2086, 2230
EarI CTCTTC 4 cut(s) 104, 1549, 2086, 2230
Eco105I TACGTA 2 cut(s) 1924, 2087
Eco130I CCWWGG 5 cut(s) 250, 730, 1079, 1155, 2271
Eco147I AGGCCT 1 cut(s) 1340
Eco24I GRGCYC 1 cut(s) 685
Eco31I GGTCTC 2 cut(s) 442, 1767
Eco47I GGWCC 4 cut(s) 608, 1046, 1553, 2280
Eco57I CTGAAG 3 cut(s) 687, 1152, 1623
Eco72I CACGTG 1 cut(s) 1550
Eco88I CYCGRG 2 cut(s) 684, 1479
EcoRI GAATTC 1 cut(s) 456
EcoRII CCWGG 3 cut(s) 948, 1600, 1888
EcoT14I CCWWGG 5 cut(s) 250, 730, 1079, 1155, 2271
EcoT22I ATGCAT 2 cut(s) 15, 47
EcoT38I GRGCYC 1 cut(s) 685
ErhI CCWWGG 5 cut(s) 250, 730, 1079, 1155, 2271
Esp3I CGTCTC 1 cut(s) 294
FaqI GGGAC 4 cut(s) 500, 1162, 1207, 1695
FauNDI CATATG 1 cut(s) 2259
FbaI TGATCA 1 cut(s) 859
FblI GTMKAC 2 cut(s) 321, 1684
FokI GGATG 7 cut(s) 54, 450, 731, 788, 926, 1737, 1928
FriOI GRGCYC 1 cut(s) 685
FspBI CTAG 4 cut(s) 251, 794, 873, 2315
GlaI GCGC 1 cut(s) 1597
GsaI CCCAGC 1 cut(s) 1257
GsuI CTGGAG 2 cut(s) 1371, 1749
HaeII RGCGCY 1 cut(s) 1599
HaeIII GGCC 7 cut(s) 574, 648, 729, 1340, 1955, 2109, 2132
HapII CCGG 2 cut(s) 400, 685
HhaI GCGC 1 cut(s) 1598
Hin6I GCGC 1 cut(s) 1596
HinP1I GCGC 1 cut(s) 1596
HincII GTYRAC 2 cut(s) 322, 2158
HindII GTYRAC 2 cut(s) 322, 2158
HindIII AAGCTT 2 cut(s) 1905, 2323
HinfI GANTC 5 cut(s) 560, 578, 707, 1055, 1651
HpaII CCGG 2 cut(s) 400, 685
HphI GGTGA 5 cut(s) 273, 284, 1620, 1850, 2089
Hpy99I CGWCG 1 cut(s) 2288
HpyAV CCTTC 2 cut(s) 421, 2046
HpyCH4III ACNGT 8 cut(s) 695, 934, 969, 1043, 1063, 1093, 1283, 2349
HpyCH4IV ACGT 7 cut(s) 66, 287, 1317, 1549, 1923, 2068, 2086
HpyF3I CTNAG 6 cut(s) 77, 141, 468, 826, 1276, 1911
HpySE526I ACGT 7 cut(s) 66, 287, 1317, 1549, 1923, 2068, 2086
HspAI GCGC 1 cut(s) 1596
Ksp22I TGATCA 1 cut(s) 859
LmnI GCTCC 7 cut(s) 149, 680, 917, 1244, 1257, 1352, 2170
LweI GCATC 8 cut(s) 22, 32, 130, 868, 892, 965, 1213, 2348
MaeI CTAG 4 cut(s) 251, 794, 873, 2315
MaeII ACGT 7 cut(s) 66, 287, 1317, 1549, 1923, 2068, 2086
MaeIII GTNAC 8 cut(s) 356, 1313, 1318, 1606, 1642, 1813, 2053, 2095
MflI RGATCY 4 cut(s) 479, 1474, 1853, 2023
MhlI GDGCHC 2 cut(s) 685, 872
MlyI GAGTC 3 cut(s) 554, 716, 1660
MmeI TCCRAC 2 cut(s) 2212, 2307
Mph1103I ATGCAT 2 cut(s) 15, 47
MroXI GAANNNNTTC 1 cut(s) 1178
MseI TTAA 9 cut(s) 23, 72, 591, 1187, 1293, 1364, 1740, 2301, 2356
MslI CAYNNNNRTG 1 cut(s) 2165
MspA1I CMGCKG 5 cut(s) 335, 1721, 1730, 1835, 2048
MspI CCGG 2 cut(s) 400, 685
MspR9I CCNGG 6 cut(s) 401, 685, 686, 950, 1602, 1890
Mva1269I GAATGC 2 cut(s) 526, 1745
MvaI CCWGG 3 cut(s) 950, 1602, 1890
NciI CCSGG 3 cut(s) 401, 685, 686
NcoI CCATGG 3 cut(s) 730, 1079, 1155
NdeI CATATG 1 cut(s) 2259
NlaIV GGNNCC 6 cut(s) 682, 736, 822, 869, 1696, 2172
NmuCI GTSAC 2 cut(s) 1313, 1318
NsiI ATGCAT 2 cut(s) 15, 47
PaeR7I CTCGAG 1 cut(s) 1479
PasI CCCWGGG 2 cut(s) 1601, 1889
PceI AGGCCT 1 cut(s) 1340
PctI GAATGC 2 cut(s) 526, 1745
PdmI GAANNNNTTC 1 cut(s) 1178
PfeI GAWTC 2 cut(s) 578, 1055
PflMI CCANNNNNTGG 2 cut(s) 624, 1003
PleI GAGTC 3 cut(s) 554, 715, 1659
PmaCI CACGTG 1 cut(s) 1550
PmlI CACGTG 1 cut(s) 1550
PpsI GAGTC 3 cut(s) 554, 715, 1659
Ppu21I YACGTR 3 cut(s) 1550, 1924, 2087
PshBI ATTAAT 1 cut(s) 72
Psp6I CCWGG 3 cut(s) 948, 1600, 1888
PspCI CACGTG 1 cut(s) 1550
PspFI CCCAGC 1 cut(s) 1253
PspGI CCWGG 3 cut(s) 948, 1600, 1888
PspN4I GGNNCC 6 cut(s) 682, 736, 822, 869, 1696, 2172
PspPI GGNCC 4 cut(s) 608, 1046, 1553, 2280
PspXI VCTCGAGB 1 cut(s) 1479
PstI CTGCAG 1 cut(s) 760
PstNI CAGNNNCTG 2 cut(s) 710, 2003
PsuI RGATCY 4 cut(s) 479, 1474, 1853, 2023
PvuII CAGCTG 3 cut(s) 1721, 1730, 2048
RsaI GTAC 8 cut(s) 62, 248, 278, 805, 1017, 1108, 1404, 1457
RsaNI GTAC 8 cut(s) 61, 247, 277, 804, 1016, 1107, 1403, 1456
RseI CAYNNNNRTG 1 cut(s) 2165
SalI GTCGAC 1 cut(s) 320
SaqAI TTAA 9 cut(s) 23, 72, 591, 1187, 1293, 1364, 1740, 2301, 2356
Sau96I GGNCC 4 cut(s) 608, 1046, 1553, 2280
ScaI AGTACT 1 cut(s) 1108
SchI GAGTC 3 cut(s) 554, 716, 1660
ScrFI CCNGG 6 cut(s) 401, 685, 686, 950, 1602, 1890
SduI GDGCHC 2 cut(s) 685, 872
SfaNI GCATC 8 cut(s) 22, 32, 130, 868, 892, 965, 1213, 2348
SfcI CTRYAG 4 cut(s) 756, 1794, 1881, 1995
Sfr274I CTCGAG 1 cut(s) 1479
SinI GGWCC 4 cut(s) 608, 1046, 1553, 2280
SlaI CTCGAG 1 cut(s) 1479
SmaI CCCGGG 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 2165
SmlI CTYRAG 4 cut(s) 193, 1479, 2151, 2175
SmoI CTYRAG 4 cut(s) 193, 1479, 2151, 2175
SnaBI TACGTA 2 cut(s) 1924, 2087
SseBI AGGCCT 1 cut(s) 1340
SsiI CCGC 6 cut(s) 333, 645, 911, 1032, 1521, 1833
SspMI CTAG 4 cut(s) 251, 794, 873, 2315
StuI AGGCCT 1 cut(s) 1340
StyD4I CCNGG 6 cut(s) 399, 683, 684, 948, 1600, 1888
StyI CCWWGG 5 cut(s) 250, 730, 1079, 1155, 2271
TaaI ACNGT 8 cut(s) 695, 934, 969, 1043, 1063, 1093, 1283, 2349
TaiI ACGT 7 cut(s) 69, 290, 1320, 1552, 1926, 2071, 2089
TaqI TCGA 4 cut(s) 124, 321, 1248, 1480
TaqII GACCGA 1 cut(s) 1930
TatI WGTACW 3 cut(s) 276, 1106, 1455
TauI GCSGC 3 cut(s) 648, 1523, 1835
TfiI GAWTC 2 cut(s) 578, 1055
Tru1I TTAA 9 cut(s) 23, 72, 591, 1187, 1293, 1364, 1740, 2301, 2356
Tru9I TTAA 9 cut(s) 23, 72, 591, 1187, 1293, 1364, 1740, 2301, 2356
TscAI CASTG 7 cut(s) 379, 400, 532, 1048, 1176, 1288, 1834
TseFI GTSAC 2 cut(s) 1313, 1318
Tsp45I GTSAC 2 cut(s) 1313, 1318
TspDTI ATGAA 9 cut(s) 237, 440, 687, 735, 927, 1527, 1823, 1917, 2342
TspGWI ACGGA 3 cut(s) 532, 1341, 2185
TspMI CCCGGG 1 cut(s) 684
TspRI CASTG 7 cut(s) 379, 400, 532, 1048, 1176, 1288, 1834
Van91I CCANNNNNTGG 2 cut(s) 624, 1003
VpaK11BI GGWCC 4 cut(s) 608, 1046, 1553, 2280
VspI ATTAAT 1 cut(s) 72
XapI RAATTY 8 cut(s) 340, 456, 567, 652, 1027, 1558, 1841, 2116
XhoI CTCGAG 1 cut(s) 1479
XmaI CCCGGG 1 cut(s) 684
XmaJI CCTAGG 1 cut(s) 250
XmiI GTMKAC 2 cut(s) 321, 1684
XmnI GAANNNNTTC 1 cut(s) 1178
XspI CTAG 4 cut(s) 251, 794, 873, 2315
ZrmI AGTACT 1 cut(s) 1108
Zsp2I ATGCAT 2 cut(s) 15, 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.