Rw1G004770

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000257
Physical Location & Seq
Reverse (-)
9113826 .. 9116222
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004770.1

Sequence Viewer

Length: 2319 bp
ATGGACAATATGAATTTGAATTGTTATGAAGGAAAAAAAAATCATATTTTGGTTAACTGCAATGCCAAAGATGAATCGTATGTTAAGGGAAATAAAATCTGGATGCATAATGGAGATGTGCATGTAGCTATCCTATTTTCTTATGCTTTCCCCACTCTTATACTAACCTTCAACTTCCTCCTGTGCCAAGCCATTGATGAAAATAGAACGGTCCACATTGTGTACCTTGGTTCACTTCCTGATAATGAGGTGTACTCACCAATGTCTCACCACCTTGGTTTACTCCAAAAAGTTGTCGACAGCAACTCCGCAGCAAATTTCTTGACAAGAAGTTACAAAAGGAGTTTCAGTGGATTTGCTGCAAAGCTCACTGACCGGGAAAGGGAAAGGCTTGCTAACATGAAGGAAGTAGTCTCTGTATTTCCAAGCAGAATTCTCCATCCTCAGACAACAAGATCTTGGGACTTTATCGGTTTCCATGAGAAAATAAAACGGAATGTCAGTGTTGAGAGTGATACCATTATTGGTGTCATTGACACTGGAATTTGGCCTGAATCGGAGAGCTTTAAAGATGATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGTGCTTGTGAAGGTGGCCAAAATTTCACTTGCAACAAGAAGCTAATTGGAGCTCGGTTTTACAATTCATCTTCAGAATCTGCAAGGGATGAACAAGGCCATGGAACCCACACAGCCTCAACTGCAGCAGGGAACGCTATAAAGGATGTGAGCTTTTATGGACTAGCACAAGGTACTACAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGCGTATAAAGTATGTGGTGCTTTTGGGTGCCCTACTGATGCTATATTGGCTGCCTTTGATGATGCTATTGCCGATGGAGTTGACATCATTACCGTTTCATTAGGAAACTCGGTTGCATCTTTATTACAGCATGACCCTATTGCTATCGGGGCTTTCCATGCAATGGCAAAGGGGATACTTACGTCAAATTCTGCAGGCAACGGTGGTCCTGTTGATTCAAGTGTTTCAAGTGTAGCACCATGGATACTTACAATTGCAGCGAGTAGTACTGATCGTAGGATCATTGACAAGGTTGTTCTCGGAAATGGGACGACCATGGTTGGGGCTTCAGTGAACACTTTCAAATTAAATGGGACAAGTTTTCCATTGATCTATGGAAAAGATGCTTCAAGTAATTGCTCCGAGTTCGAAGCTGGGAGATGTTCATACGGCTGCTTAGATAGTGGTTTAGTTAAGGGAAAGGTTGTGTTATGTGACGTGCCTAACAGAGTTGATGAGGCCTATGTTTCTGGAGCAGTAGGCTTCATTTTGAGAGTTGATTATGATGATGTTTCTGAAATTGCACCCTTTCCTACAACAGCTCTTGCCAACAAAGAGCATAGTCTGATAAAGTTGTACACGAACTCCACGAAAGATCCTCGAGTAAACATACTAAGAAGTGAAGAAGAAAGGGATACAAACGCACCTGTTGTTGCTTCCTTCTCTTCACGTGGACCAAATTTAATTCTACCAGAAATTATCAAGCCAGATATAAGCGCCCCAGGGGTTACTATTTTGGCTGCCTTTTCACCTATTGCTTCAGTTACAGAGAGTCTTCAAGACACAAGGCATGTCAAATATAGTATACTATCTGGAACCTCTATGTCTTGTCCGCATGCGGCTGGTGCAGCTGCATATGTTAAAGCATTCCATCCTAATTGGTCTCCAGCATCCATCAAATCATCTCTTATGACTACAGCTTCTCCCATGAATGTTACTGACAATAGCAATGCCGCTGGTGAATTTGCTTATGGATCTGGACATATCAATCCTGTCAAAGCTATAGACCCAGGGCTTGTGTATGAAGCTTCCAAGGAAGACTACGTAAAGTTGCTATGCTCGGTCTTGGATCAGGCCGATGTTAGACTTATATCAGGAGATAACAGCACTTGCTCTACAGGCTCTGACAAAGGATCTTTACAGGATCACAATTACCCTTCACTAGCAGCCGTTGTCACACCAAACAAATCTTTTTCGTTCAAATTTCACAGAAAAGTTAAGAATGTTGGCCTTGCAAACTCCACTTACAAGGCCACAATATTCGCCAACTCTACTCAAGTTGACGTCAAAGTGGTGCCTCAAGTTCTTTCCTTCAAGTCCTTGAATGAGGAGAAGACTTTTGATGTGACAGTTACCGGAAAAGGTTTGCCAGATGAAGTACAATCACATGTGTCTGCATCTCTGGTGTGGTCTGATGGAATTCATAACGTTAGAAGTCCAATTCTTATCCACAAAAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

772

Amino Acids

82.95

Weight (kDa)

6.74

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 71 - 147 2.1e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 171 - 612 2.1e-45 Subtilase family
fn3_6 PF17766 667 - 768 2.7e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2142
AccB1I GGYRCC 2 cut(s) 843, 2149
AccB7I CCANNNNNTGG 2 cut(s) 600, 979
AccI GTMKAC 2 cut(s) 297, 1660
AciI CCGC 4 cut(s) 309, 1688, 1694, 1809
AclI AACGTT 1 cut(s) 2283
AclWI GGATC 6 cut(s) 1103, 1445, 1837, 1932, 1996, 2007
AcoI YGGCCR 1 cut(s) 622
AcuI CTGAAG 3 cut(s) 663, 1128, 1599
AcvI CACGTG 1 cut(s) 1526
AcyI GRCGYC 1 cut(s) 2139
AdeI CACNNNGTG 1 cut(s) 220
AfaI GTAC 6 cut(s) 224, 254, 781, 1084, 1433, 2235
AfiI CCNNNNNNNGG 4 cut(s) 382, 600, 979, 1137
AflIII ACRYGT 1 cut(s) 2242
AjiI CACGTC 1 cut(s) 1294
AjnI CCWGG 2 cut(s) 1576, 1864
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 3 cut(s) 270, 418, 1743
AlwI GGATC 6 cut(s) 1103, 1445, 1837, 1932, 1996, 2007
AlwNI CAGNNNCTG 2 cut(s) 686, 1979
Ama87I CYCGRG 1 cut(s) 1455
AoxI GGCC 7 cut(s) 548, 622, 703, 1314, 1929, 2083, 2106
Asp700I GAANNNNTTC 1 cut(s) 1154
AspLEI GCGC 1 cut(s) 1574
AspS9I GGNCC 4 cut(s) 211, 584, 1022, 1529
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 4 cut(s) 249, 260, 1596, 1826
AsuII TTCGAA 1 cut(s) 1224
AvaI CYCGRG 1 cut(s) 1455
AvaII GGWCC 4 cut(s) 211, 584, 1022, 1529
BaeGI GKGCMC 1 cut(s) 848
BaeI ACNNNNGTAYC 2 cut(s) 983, 1016
BalI TGGCCA 1 cut(s) 624
BanI GGYRCC 2 cut(s) 843, 2149
BanII GRGCYC 1 cut(s) 661
BbrPI CACGTG 1 cut(s) 1526
BbsI GAAGAC 3 cut(s) 1622, 1899, 2195
Bbv12I GWGCWC 1 cut(s) 661
BbvCI CCTCAGC 1 cut(s) 802
BccI CCATC 6 cut(s) 447, 569, 884, 1734, 1757, 2264
BceAI ACGGC 2 cut(s) 1261, 2009
BciT130I CCWGG 2 cut(s) 1578, 1866
BciVI GTATCC 3 cut(s) 984, 1053, 1483
BcnI CCSGG 1 cut(s) 377
BcoDI GTCTC 3 cut(s) 270, 418, 1743
BfaI CTAG 2 cut(s) 770, 2018
BfmI CTRYAG 5 cut(s) 729, 1008, 1770, 1857, 1971
BfoI RGCGCY 1 cut(s) 1575
BfuI GTATCC 3 cut(s) 984, 1053, 1483
BglII AGATCT 1 cut(s) 455
BmcAI AGTACT 1 cut(s) 1084
Bme1390I CCNGG 3 cut(s) 377, 1578, 1866
Bme18I GGWCC 4 cut(s) 211, 584, 1022, 1529
BmeT110I CYCGRG 1 cut(s) 1455
BmgBI CACGTC 1 cut(s) 1294
BmgT120I GGNCC 4 cut(s) 211, 584, 1022, 1529
BmiI GGNNCC 5 cut(s) 712, 798, 845, 1672, 2151
BmrFI CCNGG 3 cut(s) 377, 1578, 1866
BmsI GCATC 7 cut(s) 93, 844, 868, 941, 1189, 1754, 2261
BpiI GAAGAC 3 cut(s) 1622, 1899, 2195
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpmI CTGGAG 2 cut(s) 1347, 1725
Bpu10I CCTNAGC 1 cut(s) 802
Bpu14I TTCGAA 1 cut(s) 1224
BpuEI CTTGAG 2 cut(s) 2115, 2139
BpuMI CCSGG 1 cut(s) 377
BsaAI YACGTR 2 cut(s) 1526, 1900
BsaHI GRCGYC 1 cut(s) 2139
BsaI GGTCTC 1 cut(s) 1743
BsaWI WCCGGW 1 cut(s) 2210
BsaXI ACNNNNNCTCC 8 cut(s) 290, 320, 334, 364, 1424, 1454, 1762, 1792
Bsc4I CCNNNNNNNGG 4 cut(s) 382, 600, 979, 1137
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 3 cut(s) 67, 984, 1810
BseBI CCWGG 2 cut(s) 1578, 1866
BseGI GGATG 6 cut(s) 108, 439, 700, 757, 1726, 1745
BseLI CCNNNNNNNGG 4 cut(s) 382, 600, 979, 1137
BseMI GCAATG 3 cut(s) 67, 984, 1810
BseMII CTCAG 2 cut(s) 458, 816
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 3 cut(s) 579, 804, 2198
BseSI GKGCMC 1 cut(s) 848
BseYI CCCAGC 1 cut(s) 1229
BsgI GTGCAG 1 cut(s) 1722
BshFI GGCC 7 cut(s) 550, 624, 705, 1316, 1931, 2085, 2108
BshNI GGYRCC 2 cut(s) 843, 2149
BsiHKAI GWGCWC 1 cut(s) 661
BsiHKCI CYCGRG 1 cut(s) 1455
BsiSI CCGG 2 cut(s) 376, 2211
BslFI GGGAC 3 cut(s) 476, 1138, 1183
BslI CCNNNNNNNGG 4 cut(s) 382, 600, 979, 1137
BsmAI GTCTC 3 cut(s) 270, 418, 1743
BsmFI GGGAC 3 cut(s) 476, 1138, 1183
BsmI GAATGC 1 cut(s) 1721
BsnI GGCC 7 cut(s) 550, 624, 705, 1316, 1931, 2085, 2108
Bso31I GGTCTC 1 cut(s) 1743
BsoBI CYCGRG 1 cut(s) 1455
Bsp119I TTCGAA 1 cut(s) 1224
Bsp1286I GDGCHC 2 cut(s) 661, 848
Bsp1407I TGTACA 1 cut(s) 1431
Bsp143I GATC 9 cut(s) 455, 1087, 1095, 1185, 1450, 1829, 1924, 1988, 1999
Bsp19I CCATGG 3 cut(s) 706, 1055, 1131
BspACI CCGC 4 cut(s) 309, 1688, 1694, 1809
BspANI GGCC 7 cut(s) 550, 624, 705, 1316, 1931, 2085, 2108
BspCNI CTCAG 2 cut(s) 457, 815
BspLI GGNNCC 5 cut(s) 712, 798, 845, 1672, 2151
BspMAI CTGCAG 2 cut(s) 733, 1012
BspPI GGATC 6 cut(s) 1103, 1445, 1837, 1932, 1996, 2007
BspT104I TTCGAA 1 cut(s) 1224
BspT107I GGYRCC 2 cut(s) 843, 2149
BspTNI GGTCTC 1 cut(s) 1743
BsrDI GCAATG 3 cut(s) 67, 984, 1810
BsrGI TGTACA 1 cut(s) 1431
BsrI ACTGG 1 cut(s) 544
BssMI GATC 9 cut(s) 455, 1087, 1095, 1185, 1450, 1829, 1924, 1988, 1999
BssNAI GTATAC 1 cut(s) 1661
BssNI GRCGYC 1 cut(s) 2139
BssT1I CCWWGG 6 cut(s) 226, 274, 706, 1055, 1131, 1887
Bst1107I GTATAC 1 cut(s) 1661
Bst2UI CCWGG 2 cut(s) 1578, 1866
Bst4CI ACNGT 4 cut(s) 211, 910, 1019, 2206
Bst6I CTCTTC 1 cut(s) 1525
BstACI GRCGYC 1 cut(s) 2139
BstAUI TGTACA 1 cut(s) 1431
BstBAI YACGTR 2 cut(s) 1526, 1900
BstBI TTCGAA 1 cut(s) 1224
BstC8I GCNNGC 3 cut(s) 393, 1012, 1692
BstDEI CTNAG 4 cut(s) 444, 802, 1252, 1469
BstDSI CCRYGG 3 cut(s) 706, 1055, 1131
BstF5I GGATG 6 cut(s) 108, 439, 700, 757, 1726, 1745
BstH2I RGCGCY 1 cut(s) 1575
BstHHI GCGC 1 cut(s) 1574
BstKTI GATC 9 cut(s) 458, 1090, 1098, 1188, 1453, 1832, 1927, 1991, 2002
BstMAI GTCTC 3 cut(s) 270, 418, 1743
BstMBI GATC 9 cut(s) 455, 1087, 1095, 1185, 1450, 1829, 1924, 1988, 1999
BstMWI GCNNNNNNNGC 9 cut(s) 728, 740, 812, 863, 965, 974, 1700, 1703, 1974
BstNI CCWGG 2 cut(s) 1578, 1866
BstNSI RCATGY 4 cut(s) 125, 1649, 1694, 2246
BstSCI CCNGG 3 cut(s) 375, 1576, 1864
BstSFI CTRYAG 5 cut(s) 729, 1008, 1770, 1857, 1971
BstSLI GKGCMC 1 cut(s) 848
BstSNI TACGTA 1 cut(s) 1900
BstV2I GAAGAC 3 cut(s) 1622, 1899, 2195
BstX2I RGATCY 4 cut(s) 455, 1450, 1829, 1988
BstYI RGATCY 4 cut(s) 455, 1450, 1829, 1988
BstZ17I GTATAC 1 cut(s) 1661
BsuI GTATCC 3 cut(s) 984, 1053, 1483
BsuRI GGCC 7 cut(s) 550, 624, 705, 1316, 1931, 2085, 2108
BtgI CCRYGG 3 cut(s) 706, 1055, 1131
BtrI CACGTC 1 cut(s) 1294
BtsCI GGATG 6 cut(s) 108, 439, 700, 757, 1726, 1745
BtsIMutI CAGTG 5 cut(s) 355, 369, 508, 537, 1152
Cac8I GCNNGC 3 cut(s) 393, 1012, 1692
CaiI CAGNNNCTG 2 cut(s) 686, 1979
CfoI GCGC 1 cut(s) 1574
Cfr13I GGNCC 4 cut(s) 211, 584, 1022, 1529
Csp6I GTAC 6 cut(s) 223, 253, 780, 1083, 1432, 2234
CviQI GTAC 6 cut(s) 223, 253, 780, 1083, 1432, 2234
DdeI CTNAG 4 cut(s) 444, 802, 1252, 1469
DpnI GATC 9 cut(s) 457, 1089, 1097, 1187, 1452, 1831, 1926, 1990, 2001
DpnII GATC 9 cut(s) 455, 1087, 1095, 1185, 1450, 1829, 1924, 1988, 1999
DraI TTTAAA 1 cut(s) 568
DraIII CACNNNGTG 1 cut(s) 220
EaeI YGGCCR 1 cut(s) 622
Eam1104I CTCTTC 1 cut(s) 1525
EarI CTCTTC 1 cut(s) 1525
Ecl136II GAGCTC 1 cut(s) 659
Eco105I TACGTA 1 cut(s) 1900
Eco130I CCWWGG 6 cut(s) 226, 274, 706, 1055, 1131, 1887
Eco147I AGGCCT 1 cut(s) 1316
Eco24I GRGCYC 1 cut(s) 661
Eco31I GGTCTC 1 cut(s) 1743
Eco47I GGWCC 4 cut(s) 211, 584, 1022, 1529
Eco53kI GAGCTC 1 cut(s) 659
Eco57I CTGAAG 3 cut(s) 663, 1128, 1599
Eco72I CACGTG 1 cut(s) 1526
Eco88I CYCGRG 1 cut(s) 1455
EcoICRI GAGCTC 1 cut(s) 659
EcoRI GAATTC 2 cut(s) 432, 2274
EcoRII CCWGG 2 cut(s) 1576, 1864
EcoT14I CCWWGG 6 cut(s) 226, 274, 706, 1055, 1131, 1887
EcoT22I ATGCAT 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 661
ErhI CCWWGG 6 cut(s) 226, 274, 706, 1055, 1131, 1887
FaqI GGGAC 3 cut(s) 476, 1138, 1183
FauNDI CATATG 1 cut(s) 1711
FblI GTMKAC 2 cut(s) 297, 1660
FokI GGATG 6 cut(s) 115, 426, 707, 764, 1713, 1732
FriOI GRGCYC 1 cut(s) 661
FspBI CTAG 2 cut(s) 770, 2018
GlaI GCGC 1 cut(s) 1573
GsaI CCCAGC 1 cut(s) 1233
GsuI CTGGAG 2 cut(s) 1347, 1725
HaeII RGCGCY 1 cut(s) 1575
HaeIII GGCC 7 cut(s) 550, 624, 705, 1316, 1931, 2085, 2108
HapII CCGG 2 cut(s) 376, 2211
HhaI GCGC 1 cut(s) 1574
Hin1I GRCGYC 1 cut(s) 2139
Hin6I GCGC 1 cut(s) 1572
HinP1I GCGC 1 cut(s) 1572
HincII GTYRAC 4 cut(s) 55, 298, 898, 2137
HindII GTYRAC 4 cut(s) 55, 298, 898, 2137
HindIII AAGCTT 1 cut(s) 1881
HinfI GANTC 5 cut(s) 74, 554, 683, 1031, 1627
HpaI GTTAAC 1 cut(s) 55
HpaII CCGG 2 cut(s) 376, 2211
HphI GGTGA 4 cut(s) 249, 260, 1596, 1826
Hpy188I TCNGA 9 cut(s) 447, 559, 682, 1118, 1219, 1372, 1422, 1981, 2269
Hpy188III TCNNGA 8 cut(s) 100, 239, 322, 1326, 1634, 1668, 1833, 1950
HpyAV CCTTC 7 cut(s) 23, 178, 397, 611, 1525, 2022, 2176
HpyCH4III ACNGT 4 cut(s) 211, 910, 1019, 2206
HpyCH4IV ACGT 6 cut(s) 998, 1293, 1525, 1899, 2139, 2283
HpyF10VI GCNNNNNNNGC 9 cut(s) 728, 740, 812, 863, 965, 974, 1700, 1703, 1974
HpyF3I CTNAG 4 cut(s) 444, 802, 1252, 1469
HpySE526I ACGT 6 cut(s) 998, 1293, 1525, 1899, 2139, 2283
Hsp92I GRCGYC 1 cut(s) 2139
HspAI GCGC 1 cut(s) 1572
KspAI GTTAAC 1 cut(s) 55
Kzo9I GATC 9 cut(s) 455, 1087, 1095, 1185, 1450, 1829, 1924, 1988, 1999
LmnI GCTCC 3 cut(s) 656, 1220, 1328
LweI GCATC 7 cut(s) 93, 844, 868, 941, 1189, 1754, 2261
MaeI CTAG 2 cut(s) 770, 2018
MaeII ACGT 6 cut(s) 998, 1293, 1525, 1899, 2139, 2283
MaeIII GTNAC 8 cut(s) 332, 1289, 1582, 1618, 1789, 2029, 2200, 2206
MalI GATC 9 cut(s) 457, 1089, 1097, 1187, 1452, 1831, 1926, 1990, 2001
MboI GATC 9 cut(s) 455, 1087, 1095, 1185, 1450, 1829, 1924, 1988, 1999
MboII GAAGA 7 cut(s) 669, 1490, 1493, 1512, 1622, 1904, 2200
MfeI CAATTG 1 cut(s) 1068
MflI RGATCY 4 cut(s) 455, 1450, 1829, 1988
MhlI GDGCHC 2 cut(s) 661, 848
MlsI TGGCCA 1 cut(s) 624
MluNI TGGCCA 1 cut(s) 624
MlyI GAGTC 1 cut(s) 1636
Mox20I TGGCCA 1 cut(s) 624
Mph1103I ATGCAT 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 1154
MscI TGGCCA 1 cut(s) 624
MseI TTAA 8 cut(s) 54, 84, 567, 1163, 1269, 1538, 1716, 2073
Msp20I TGGCCA 1 cut(s) 624
MspA1I CMGCKG 2 cut(s) 1706, 1811
MspI CCGG 2 cut(s) 376, 2211
MspR9I CCNGG 3 cut(s) 377, 1578, 1866
MunI CAATTG 1 cut(s) 1068
Mva1269I GAATGC 1 cut(s) 1721
MvaI CCWGG 2 cut(s) 1578, 1866
MwoI GCNNNNNNNGC 9 cut(s) 728, 740, 812, 863, 965, 974, 1700, 1703, 1974
NciI CCSGG 1 cut(s) 377
NcoI CCATGG 3 cut(s) 706, 1055, 1131
NdeI CATATG 1 cut(s) 1711
NdeII GATC 9 cut(s) 455, 1087, 1095, 1185, 1450, 1829, 1924, 1988, 1999
NlaIV GGNNCC 5 cut(s) 712, 798, 845, 1672, 2151
NmuCI GTSAC 3 cut(s) 1289, 2029, 2200
NsiI ATGCAT 1 cut(s) 108
NspI RCATGY 4 cut(s) 125, 1649, 1694, 2246
NspV TTCGAA 1 cut(s) 1224
PaeI GCATGC 1 cut(s) 1694
PaeR7I CTCGAG 1 cut(s) 1455
PasI CCCWGGG 2 cut(s) 1577, 1865
PceI AGGCCT 1 cut(s) 1316
PciI ACATGT 1 cut(s) 2242
PcsI WCGNNNNNNNCGW 1 cut(s) 1442
PctI GAATGC 1 cut(s) 1721
PdmI GAANNNNTTC 1 cut(s) 1154
PfeI GAWTC 4 cut(s) 74, 554, 683, 1031
PflMI CCANNNNNTGG 2 cut(s) 600, 979
PleI GAGTC 1 cut(s) 1635
PmaCI CACGTG 1 cut(s) 1526
PmlI CACGTG 1 cut(s) 1526
PpsI GAGTC 1 cut(s) 1635
Ppu21I YACGTR 2 cut(s) 1526, 1900
PscI ACATGT 1 cut(s) 2242
Psp124BI GAGCTC 1 cut(s) 661
Psp1406I AACGTT 1 cut(s) 2283
Psp6I CCWGG 2 cut(s) 1576, 1864
PspCI CACGTG 1 cut(s) 1526
PspFI CCCAGC 1 cut(s) 1229
PspGI CCWGG 2 cut(s) 1576, 1864
PspN4I GGNNCC 5 cut(s) 712, 798, 845, 1672, 2151
PspPI GGNCC 4 cut(s) 211, 584, 1022, 1529
PspXI VCTCGAGB 1 cut(s) 1455
PstI CTGCAG 2 cut(s) 733, 1012
PstNI CAGNNNCTG 2 cut(s) 686, 1979
PsuI RGATCY 4 cut(s) 455, 1450, 1829, 1988
PvuII CAGCTG 1 cut(s) 1706
RsaI GTAC 6 cut(s) 224, 254, 781, 1084, 1433, 2235
RsaNI GTAC 6 cut(s) 223, 253, 780, 1083, 1432, 2234
SacI GAGCTC 1 cut(s) 661
SalI GTCGAC 1 cut(s) 296
SaqAI TTAA 8 cut(s) 54, 84, 567, 1163, 1269, 1538, 1716, 2073
Sau3AI GATC 9 cut(s) 455, 1087, 1095, 1185, 1450, 1829, 1924, 1988, 1999
Sau96I GGNCC 4 cut(s) 211, 584, 1022, 1529
ScaI AGTACT 1 cut(s) 1084
SchI GAGTC 1 cut(s) 1636
ScrFI CCNGG 3 cut(s) 377, 1578, 1866
SduI GDGCHC 2 cut(s) 661, 848
SfaNI GCATC 7 cut(s) 93, 844, 868, 941, 1189, 1754, 2261
SfcI CTRYAG 5 cut(s) 729, 1008, 1770, 1857, 1971
Sfr274I CTCGAG 1 cut(s) 1455
SfuI TTCGAA 1 cut(s) 1224
SinI GGWCC 4 cut(s) 211, 584, 1022, 1529
SlaI CTCGAG 1 cut(s) 1455
SmlI CTYRAG 3 cut(s) 1455, 2130, 2154
SmoI CTYRAG 3 cut(s) 1455, 2130, 2154
SnaBI TACGTA 1 cut(s) 1900
SphI GCATGC 1 cut(s) 1694
SseBI AGGCCT 1 cut(s) 1316
SsiI CCGC 4 cut(s) 309, 1688, 1694, 1809
SspI AATATT 1 cut(s) 2115
SspMI CTAG 2 cut(s) 770, 2018
SstI GAGCTC 1 cut(s) 661
StuI AGGCCT 1 cut(s) 1316
StyD4I CCNGG 3 cut(s) 375, 1576, 1864
StyI CCWWGG 6 cut(s) 226, 274, 706, 1055, 1131, 1887
TaaI ACNGT 4 cut(s) 211, 910, 1019, 2206
TaiI ACGT 6 cut(s) 1001, 1296, 1528, 1902, 2142, 2286
TaqI TCGA 3 cut(s) 297, 1224, 1456
TaqII GACCGA 1 cut(s) 1906
TatI WGTACW 4 cut(s) 252, 1082, 1431, 2233
TauI GCSGC 2 cut(s) 1697, 1811
TfiI GAWTC 4 cut(s) 74, 554, 683, 1031
Tru1I TTAA 8 cut(s) 54, 84, 567, 1163, 1269, 1538, 1716, 2073
Tru9I TTAA 8 cut(s) 54, 84, 567, 1163, 1269, 1538, 1716, 2073
TscAI CASTG 5 cut(s) 355, 376, 508, 544, 1152
TseFI GTSAC 3 cut(s) 1289, 2029, 2200
Tsp45I GTSAC 3 cut(s) 1289, 2029, 2200
TspGWI ACGGA 1 cut(s) 508
TspRI CASTG 5 cut(s) 355, 376, 508, 544, 1152
Van91I CCANNNNNTGG 2 cut(s) 600, 979
VpaK11BI GGWCC 4 cut(s) 211, 584, 1022, 1529
XceI RCATGY 4 cut(s) 125, 1649, 1694, 2246
XhoI CTCGAG 1 cut(s) 1455
XmiI GTMKAC 2 cut(s) 297, 1660
XmnI GAANNNNTTC 1 cut(s) 1154
XspI CTAG 2 cut(s) 770, 2018
ZraI GACGTC 1 cut(s) 2140
ZrmI AGTACT 1 cut(s) 1084
Zsp2I ATGCAT 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.