Subtilisin-like serine endopeptidase family protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 571 |
| AccII | CGCG | 1 cut(s) | 1405 |
| AccIII | TCCGGA | 1 cut(s) | 1547 |
| AciI | CCGC | 2 cut(s) | 1525, 1977 |
| AcoI | YGGCCR | 2 cut(s) | 589, 1302 |
| AcsI | RAATTY | 4 cut(s) | 517, 1419, 1697, 1972 |
| AcuI | CTGAAG | 3 cut(s) | 720, 1011, 1404 |
| AfaI | GTAC | 6 cut(s) | 113, 143, 667, 922, 1535, 2126 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 445, 917, 1274 |
| AflIII | ACRYGT | 1 cut(s) | 1922 |
| AgsI | TTSAA | 4 cut(s) | 238, 335, 1048, 2047 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 790 |
| AhlI | ACTAGT | 1 cut(s) | 1015 |
| AjnI | CCWGG | 3 cut(s) | 661, 910, 1744 |
| Alw21I | GWGCWC | 3 cut(s) | 550, 734, 1213 |
| Alw26I | GTCTC | 4 cut(s) | 368, 802, 1509, 1623 |
| Alw44I | GTGCAC | 1 cut(s) | 1209 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 572, 1845, 1859 |
| Ama87I | CYCGRG | 1 cut(s) | 1456 |
| Aor13HI | TCCGGA | 1 cut(s) | 1547 |
| AoxI | GGCC | 9 cut(s) | 75, 589, 993, 1175, 1302, 1453, 1658, 1963, 1986 |
| ApaLI | GTGCAC | 1 cut(s) | 1209 |
| ApeKI | GCWGC | 9 cut(s) | 248, 476, 617, 701, 752, 959, 1376, 1475, 1586 |
| ApoI | RAATTY | 4 cut(s) | 517, 1419, 1697, 1972 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2059, 2091 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1037 |
| AspLEI | GCGC | 1 cut(s) | 940 |
| AspS9I | GGNCC | 5 cut(s) | 153, 908, 1176, 1409, 1454 |
| AsuC2I | CCSGG | 3 cut(s) | 1457, 1458, 1692 |
| AsuHPI | GGTGA | 4 cut(s) | 152, 310, 1706, 1945 |
| AvaI | CYCGRG | 1 cut(s) | 1456 |
| AvaII | GGWCC | 3 cut(s) | 153, 908, 1409 |
| BaeGI | GKGCMC | 1 cut(s) | 1213 |
| BalI | TGGCCA | 1 cut(s) | 1304 |
| BamHI | GGATCC | 1 cut(s) | 1709 |
| BanII | GRGCYC | 1 cut(s) | 550 |
| BbsI | GAAGAC | 4 cut(s) | 591, 1296, 2072, 2112 |
| Bbv12I | GWGCWC | 3 cut(s) | 550, 734, 1213 |
| BbvCI | CCTCAGC | 1 cut(s) | 688 |
| BbvI | GCAGC | 9 cut(s) | 235, 463, 629, 688, 739, 971, 1363, 1462, 1573 |
| BccI | CCATC | 6 cut(s) | 165, 182, 770, 1118, 1630, 2099 |
| BceAI | ACGGC | 4 cut(s) | 604, 630, 925, 980 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 752, 786, 1088, 1122 |
| BciT130I | CCWGG | 3 cut(s) | 663, 912, 1746 |
| BclI | TGATCA | 2 cut(s) | 82, 262 |
| BcnI | CCSGG | 3 cut(s) | 1457, 1458, 1692 |
| BcoDI | GTCTC | 4 cut(s) | 368, 802, 1509, 1623 |
| BcuI | ACTAGT | 1 cut(s) | 1015 |
| BfaI | CTAG | 5 cut(s) | 315, 1016, 1110, 1133, 1512 |
| BfmI | CTRYAG | 7 cut(s) | 564, 723, 840, 894, 1650, 1767, 1851 |
| BfoI | RGCGCY | 1 cut(s) | 941 |
| BfuAI | ACCTGC | 1 cut(s) | 571 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 614 |
| BisI | GCNGC | 9 cut(s) | 249, 477, 618, 702, 753, 960, 1377, 1476, 1587 |
| BlpI | GCTNAGC | 1 cut(s) | 6 |
| BlsI | GCNGC | 9 cut(s) | 250, 478, 619, 703, 754, 961, 1378, 1477, 1588 |
| BmcAI | AGTACT | 1 cut(s) | 143 |
| Bme1390I | CCNGG | 6 cut(s) | 663, 912, 1457, 1458, 1692, 1746 |
| Bme18I | GGWCC | 3 cut(s) | 153, 908, 1409 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 790 |
| BmeT110I | CYCGRG | 1 cut(s) | 1456 |
| BmgT120I | GGNCC | 5 cut(s) | 153, 908, 1176, 1409, 1454 |
| BmiI | GGNNCC | 6 cut(s) | 155, 598, 684, 1552, 1711, 2028 |
| BmrFI | CCNGG | 6 cut(s) | 663, 912, 1457, 1458, 1692, 1746 |
| BmsI | GCATC | 6 cut(s) | 754, 893, 936, 1069, 1237, 1750 |
| BpiI | GAAGAC | 4 cut(s) | 591, 1296, 2072, 2112 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 128, 160, 287, 319 |
| BpmI | CTGGAG | 1 cut(s) | 933 |
| Bpu10I | CCTNAGC | 1 cut(s) | 688 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 6 |
| BpuEI | CTTGAG | 4 cut(s) | 350, 1320, 1992, 2016 |
| BpuMI | CCSGG | 3 cut(s) | 1457, 1458, 1692 |
| BsaAI | YACGTR | 1 cut(s) | 1943 |
| BsaBI | GATNNNNATC | 1 cut(s) | 834 |
| BsaI | GGTCTC | 2 cut(s) | 368, 1623 |
| BsaJI | CCNNGG | 7 cut(s) | 149, 592, 941, 1456, 1690, 1744, 1745 |
| BsaWI | WCCGGW | 2 cut(s) | 1547, 1712 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 445, 917, 1274 |
| Bse118I | RCCGGY | 1 cut(s) | 1173 |
| Bse1I | ACTGG | 3 cut(s) | 433, 1456, 1582 |
| Bse3DI | GCAATG | 1 cut(s) | 870 |
| Bse8I | GATNNNNATC | 1 cut(s) | 834 |
| BseAI | TCCGGA | 1 cut(s) | 1547 |
| BseBI | CCWGG | 3 cut(s) | 663, 912, 1746 |
| BseDI | CCNNGG | 7 cut(s) | 149, 592, 941, 1456, 1690, 1744, 1745 |
| BseGI | GGATG | 3 cut(s) | 586, 643, 951 |
| BseJI | GATNNNNATC | 1 cut(s) | 834 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 445, 917, 1274 |
| BseMI | GCAATG | 1 cut(s) | 870 |
| BseMII | CTCAG | 3 cut(s) | 271, 387, 702 |
| BseNI | ACTGG | 3 cut(s) | 433, 1456, 1582 |
| BseRI | GAGGAG | 3 cut(s) | 152, 690, 2075 |
| BseSI | GKGCMC | 1 cut(s) | 1213 |
| BseXI | GCAGC | 9 cut(s) | 235, 463, 629, 688, 739, 971, 1363, 1462, 1573 |
| BsgI | GTGCAG | 2 cut(s) | 1140, 1362 |
| Bsh1236I | CGCG | 1 cut(s) | 1405 |
| BshFI | GGCC | 9 cut(s) | 77, 591, 995, 1177, 1304, 1455, 1660, 1965, 1988 |
| BsiHKAI | GWGCWC | 3 cut(s) | 550, 734, 1213 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1456 |
| BsiSI | CCGG | 5 cut(s) | 1174, 1457, 1548, 1692, 1713 |
| BslFI | GGGAC | 4 cut(s) | 139, 365, 801, 1244 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 445, 917, 1274 |
| BsmAI | GTCTC | 4 cut(s) | 368, 802, 1509, 1623 |
| BsmFI | GGGAC | 4 cut(s) | 139, 365, 801, 1244 |
| BsmI | GAATGC | 1 cut(s) | 17 |
| BsnI | GGCC | 9 cut(s) | 77, 591, 995, 1177, 1304, 1455, 1660, 1965, 1988 |
| Bso31I | GGTCTC | 2 cut(s) | 368, 1623 |
| BsoBI | CYCGRG | 1 cut(s) | 1456 |
| Bsp1286I | GDGCHC | 3 cut(s) | 550, 734, 1213 |
| Bsp13I | TCCGGA | 1 cut(s) | 1547 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 6 |
| Bsp19I | CCATGG | 1 cut(s) | 592 |
| BspACI | CCGC | 2 cut(s) | 1525, 1977 |
| BspANI | GGCC | 9 cut(s) | 77, 591, 995, 1177, 1304, 1455, 1660, 1965, 1988 |
| BspCNI | CTCAG | 3 cut(s) | 270, 388, 701 |
| BspEI | TCCGGA | 1 cut(s) | 1547 |
| BspFNI | CGCG | 1 cut(s) | 1405 |
| BspHI | TCATGA | 3 cut(s) | 85, 265, 1644 |
| BspLI | GGNNCC | 6 cut(s) | 155, 598, 684, 1552, 1711, 2028 |
| BspMAI | CTGCAG | 3 cut(s) | 568, 898, 1771 |
| BspMI | ACCTGC | 1 cut(s) | 571 |
| BspQI | GCTCTTC | 1 cut(s) | 739 |
| BspTNI | GGTCTC | 2 cut(s) | 368, 1623 |
| BsrDI | GCAATG | 1 cut(s) | 870 |
| BsrFI | RCCGGY | 1 cut(s) | 1173 |
| BsrI | ACTGG | 3 cut(s) | 433, 1456, 1582 |
| BssAI | RCCGGY | 1 cut(s) | 1173 |
| BssECI | CCNNGG | 7 cut(s) | 149, 592, 941, 1456, 1690, 1744, 1745 |
| BssT1I | CCWWGG | 2 cut(s) | 149, 592 |
| Bst2UI | CCWGG | 3 cut(s) | 663, 912, 1746 |
| Bst4CI | ACNGT | 4 cut(s) | 905, 925, 1139, 1933 |
| Bst6I | CTCTTC | 4 cut(s) | 739, 1405, 1942, 2086 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 572 |
| BstBAI | YACGTR | 1 cut(s) | 1943 |
| BstC8I | GCNNGC | 4 cut(s) | 898, 951, 1269, 1342 |
| BstDEI | CTNAG | 4 cut(s) | 6, 257, 396, 688 |
| BstDSI | CCRYGG | 2 cut(s) | 592, 941 |
| BstF5I | GGATG | 3 cut(s) | 586, 643, 951 |
| BstFNI | CGCG | 1 cut(s) | 1405 |
| BstH2I | RGCGCY | 1 cut(s) | 941 |
| BstHHI | GCGC | 1 cut(s) | 940 |
| BstMAI | GTCTC | 4 cut(s) | 368, 802, 1509, 1623 |
| BstNI | CCWGG | 3 cut(s) | 663, 912, 1746 |
| BstSCI | CCNGG | 6 cut(s) | 661, 910, 1455, 1456, 1690, 1744 |
| BstSFI | CTRYAG | 7 cut(s) | 564, 723, 840, 894, 1650, 1767, 1851 |
| BstSLI | GKGCMC | 1 cut(s) | 1213 |
| BstSNI | TACGTA | 1 cut(s) | 1943 |
| BstUI | CGCG | 1 cut(s) | 1405 |
| BstV1I | GCAGC | 9 cut(s) | 235, 463, 629, 688, 739, 971, 1363, 1462, 1573 |
| BstV2I | GAAGAC | 4 cut(s) | 591, 1296, 2072, 2112 |
| BstX2I | RGATCY | 5 cut(s) | 1283, 1330, 1369, 1709, 1879 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1577 |
| BstYI | RGATCY | 5 cut(s) | 1283, 1330, 1369, 1709, 1879 |
| BsuRI | GGCC | 9 cut(s) | 77, 591, 995, 1177, 1304, 1455, 1660, 1965, 1988 |
| BtgI | CCRYGG | 2 cut(s) | 592, 941 |
| BtsCI | GGATG | 3 cut(s) | 586, 643, 951 |
| BtsI | GCAGTG | 2 cut(s) | 390, 603 |
| BtsIMutI | CAGTG | 7 cut(s) | 265, 390, 426, 603, 910, 1035, 1061 |
| BveI | ACCTGC | 1 cut(s) | 571 |
| Cac8I | GCNNGC | 4 cut(s) | 898, 951, 1269, 1342 |
| CaiI | CAGNNNCTG | 3 cut(s) | 572, 1845, 1859 |
| CciI | TCATGA | 3 cut(s) | 85, 265, 1644 |
| CfoI | GCGC | 1 cut(s) | 940 |
| Cfr10I | RCCGGY | 1 cut(s) | 1173 |
| Cfr13I | GGNCC | 5 cut(s) | 153, 908, 1176, 1409, 1454 |
| Cfr9I | CCCGGG | 1 cut(s) | 1456 |
| CsiI | ACCWGGT | 1 cut(s) | 661 |
| Csp6I | GTAC | 6 cut(s) | 112, 142, 666, 921, 1534, 2125 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1595, 1630 |
| CviQI | GTAC | 6 cut(s) | 112, 142, 666, 921, 1534, 2125 |
| DdeI | CTNAG | 4 cut(s) | 6, 257, 396, 688 |
| DriI | GACNNNNNGTC | 1 cut(s) | 790 |
| EaeI | YGGCCR | 2 cut(s) | 589, 1302 |
| Eam1104I | CTCTTC | 4 cut(s) | 739, 1405, 1942, 2086 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 790 |
| EarI | CTCTTC | 4 cut(s) | 739, 1405, 1942, 2086 |
| Ecl136II | GAGCTC | 1 cut(s) | 548 |
| Eco105I | TACGTA | 1 cut(s) | 1943 |
| Eco130I | CCWWGG | 2 cut(s) | 149, 592 |
| Eco24I | GRGCYC | 1 cut(s) | 550 |
| Eco31I | GGTCTC | 2 cut(s) | 368, 1623 |
| Eco47I | GGWCC | 3 cut(s) | 153, 908, 1409 |
| Eco53kI | GAGCTC | 1 cut(s) | 548 |
| Eco57I | CTGAAG | 3 cut(s) | 720, 1011, 1404 |
| Eco88I | CYCGRG | 1 cut(s) | 1456 |
| EcoICRI | GAGCTC | 1 cut(s) | 548 |
| EcoO109I | RGGNCCY | 1 cut(s) | 153 |
| EcoRII | CCWGG | 3 cut(s) | 661, 910, 1744 |
| EcoT14I | CCWWGG | 2 cut(s) | 149, 592 |
| EcoT38I | GRGCYC | 1 cut(s) | 550 |
| ErhI | CCWWGG | 2 cut(s) | 149, 592 |
| FaqI | GGGAC | 4 cut(s) | 139, 365, 801, 1244 |
| FauI | CCCGC | 1 cut(s) | 1518 |
| FauNDI | CATATG | 3 cut(s) | 1591, 1705, 2115 |
| FbaI | TGATCA | 2 cut(s) | 82, 262 |
| Fnu4HI | GCNGC | 9 cut(s) | 249, 477, 618, 702, 753, 960, 1377, 1476, 1587 |
| FokI | GGATG | 3 cut(s) | 593, 650, 958 |
| FriOI | GRGCYC | 1 cut(s) | 550 |
| Fsp4HI | GCNGC | 9 cut(s) | 249, 477, 618, 702, 753, 960, 1377, 1476, 1587 |
| FspBI | CTAG | 5 cut(s) | 315, 1016, 1110, 1133, 1512 |
| GlaI | GCGC | 1 cut(s) | 939 |
| GluI | GCNGC | 9 cut(s) | 249, 477, 618, 702, 753, 960, 1377, 1476, 1587 |
| GsuI | CTGGAG | 1 cut(s) | 933 |
| HaeII | RGCGCY | 1 cut(s) | 941 |
| HaeIII | GGCC | 9 cut(s) | 77, 591, 995, 1177, 1304, 1455, 1660, 1965, 1988 |
| HapII | CCGG | 5 cut(s) | 1174, 1457, 1548, 1692, 1713 |
| HhaI | GCGC | 1 cut(s) | 940 |
| Hin6I | GCGC | 1 cut(s) | 938 |
| HinP1I | GCGC | 1 cut(s) | 938 |
| HincII | GTYRAC | 2 cut(s) | 784, 2014 |
| HindII | GTYRAC | 2 cut(s) | 784, 2014 |
| HindIII | AAGCTT | 1 cut(s) | 643 |
| HinfI | GANTC | 4 cut(s) | 443, 569, 1666, 1815 |
| HpaII | CCGG | 5 cut(s) | 1174, 1457, 1548, 1692, 1713 |
| HphI | GGTGA | 4 cut(s) | 152, 310, 1706, 1945 |
| Hpy166II | GTNNAC | 7 cut(s) | 112, 784, 1033, 1211, 1409, 2014, 2056 |
| Hpy188I | TCNGA | 7 cut(s) | 448, 739, 981, 1288, 1485, 1861, 2140 |
| Hpy188III | TCNNGA | 8 cut(s) | 86, 128, 266, 1182, 1512, 1548, 1645, 1830 |
| Hpy8I | GTNNAC | 7 cut(s) | 112, 784, 1033, 1211, 1409, 2014, 2056 |
| Hpy99I | CGWCG | 2 cut(s) | 343, 981 |
| HpyAV | CCTTC | 6 cut(s) | 334, 458, 500, 830, 1902, 2053 |
| HpyCH4III | ACNGT | 4 cut(s) | 905, 925, 1139, 1933 |
| HpyCH4IV | ACGT | 2 cut(s) | 1924, 1942 |
| HpyF3I | CTNAG | 4 cut(s) | 6, 257, 396, 688 |
| HpySE526I | ACGT | 2 cut(s) | 1924, 1942 |
| HspAI | GCGC | 1 cut(s) | 938 |
| Kpn2I | TCCGGA | 1 cut(s) | 1547 |
| Ksp22I | TGATCA | 2 cut(s) | 82, 262 |
| LguI | GCTCTTC | 1 cut(s) | 739 |
| LmnI | GCTCC | 6 cut(s) | 14, 231, 484, 545, 914, 2026 |
| Lsp1109I | GCAGC | 9 cut(s) | 235, 463, 629, 688, 739, 971, 1363, 1462, 1573 |
| LweI | GCATC | 6 cut(s) | 754, 893, 936, 1069, 1237, 1750 |
| MabI | ACCWGGT | 1 cut(s) | 661 |
| MaeI | CTAG | 5 cut(s) | 315, 1016, 1110, 1133, 1512 |
| MaeII | ACGT | 2 cut(s) | 1924, 1942 |
| MfeI | CAATTG | 2 cut(s) | 768, 846 |
| MflI | RGATCY | 5 cut(s) | 1283, 1330, 1369, 1709, 1879 |
| MhlI | GDGCHC | 3 cut(s) | 550, 734, 1213 |
| MlsI | TGGCCA | 1 cut(s) | 1304 |
| MluNI | TGGCCA | 1 cut(s) | 1304 |
| MlyI | GAGTC | 2 cut(s) | 578, 1824 |
| MmeI | TCCRAC | 2 cut(s) | 959, 2068 |
| Mox20I | TGGCCA | 1 cut(s) | 1304 |
| MroI | TCCGGA | 1 cut(s) | 1547 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1037 |
| MscI | TGGCCA | 1 cut(s) | 1304 |
| MseI | TTAA | 3 cut(s) | 1437, 1632, 1782 |
| Msp20I | TGGCCA | 1 cut(s) | 1304 |
| MspA1I | CMGCKG | 1 cut(s) | 1376 |
| MspI | CCGG | 5 cut(s) | 1174, 1457, 1548, 1692, 1713 |
| MspR9I | CCNGG | 6 cut(s) | 663, 912, 1457, 1458, 1692, 1746 |
| MunI | CAATTG | 2 cut(s) | 768, 846 |
| Mva1269I | GAATGC | 1 cut(s) | 17 |
| MvaI | CCWGG | 3 cut(s) | 663, 912, 1746 |
| MvnI | CGCG | 1 cut(s) | 1405 |
| NciI | CCSGG | 3 cut(s) | 1457, 1458, 1692 |
| NcoI | CCATGG | 1 cut(s) | 592 |
| NdeI | CATATG | 3 cut(s) | 1591, 1705, 2115 |
| NlaIV | GGNNCC | 6 cut(s) | 155, 598, 684, 1552, 1711, 2028 |
| NmuCI | GTSAC | 5 cut(s) | 120, 388, 1169, 1462, 1498 |
| PagI | TCATGA | 3 cut(s) | 85, 265, 1644 |
| PasI | CCCWGGG | 1 cut(s) | 1745 |
| PciSI | GCTCTTC | 1 cut(s) | 739 |
| PctI | GAATGC | 1 cut(s) | 17 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1037 |
| PfeI | GAWTC | 2 cut(s) | 443, 1666 |
| PflFI | GACNNNGTC | 1 cut(s) | 343 |
| PfoI | TCCNGGA | 1 cut(s) | 910 |
| PkrI | GCNGC | 9 cut(s) | 250, 478, 619, 703, 754, 961, 1378, 1477, 1588 |
| PleI | GAGTC | 2 cut(s) | 577, 1823 |
| PpsI | GAGTC | 2 cut(s) | 577, 1823 |
| Ppu21I | YACGTR | 1 cut(s) | 1943 |
| PpuMI | RGGWCCY | 1 cut(s) | 153 |
| Psp124BI | GAGCTC | 1 cut(s) | 550 |
| Psp5II | RGGWCCY | 1 cut(s) | 153 |
| Psp6I | CCWGG | 3 cut(s) | 661, 910, 1744 |
| PspGI | CCWGG | 3 cut(s) | 661, 910, 1744 |
| PspN4I | GGNNCC | 6 cut(s) | 155, 598, 684, 1552, 1711, 2028 |
| PspPI | GGNCC | 5 cut(s) | 153, 908, 1176, 1409, 1454 |
| PspPPI | RGGWCCY | 1 cut(s) | 153 |
| PstI | CTGCAG | 3 cut(s) | 568, 898, 1771 |
| PstNI | CAGNNNCTG | 3 cut(s) | 572, 1845, 1859 |
| PsuI | RGATCY | 5 cut(s) | 1283, 1330, 1369, 1709, 1879 |
| PsyI | GACNNNGTC | 1 cut(s) | 343 |
| PvuII | CAGCTG | 1 cut(s) | 1376 |
| RsaI | GTAC | 6 cut(s) | 113, 143, 667, 922, 1535, 2126 |
| RsaNI | GTAC | 6 cut(s) | 112, 142, 666, 921, 1534, 2125 |
| SacI | GAGCTC | 1 cut(s) | 550 |
| SapI | GCTCTTC | 1 cut(s) | 739 |
| SaqAI | TTAA | 3 cut(s) | 1437, 1632, 1782 |
| SatI | GCNGC | 9 cut(s) | 249, 477, 618, 702, 753, 960, 1377, 1476, 1587 |
| Sau96I | GGNCC | 5 cut(s) | 153, 908, 1176, 1409, 1454 |
| ScaI | AGTACT | 1 cut(s) | 143 |
| SchI | GAGTC | 2 cut(s) | 578, 1824 |
| ScrFI | CCNGG | 6 cut(s) | 663, 912, 1457, 1458, 1692, 1746 |
| SduI | GDGCHC | 3 cut(s) | 550, 734, 1213 |
| SexAI | ACCWGGT | 1 cut(s) | 661 |
| SfaNI | GCATC | 6 cut(s) | 754, 893, 936, 1069, 1237, 1750 |
| SfcI | CTRYAG | 7 cut(s) | 564, 723, 840, 894, 1650, 1767, 1851 |
| SinI | GGWCC | 3 cut(s) | 153, 908, 1409 |
| SmaI | CCCGGG | 1 cut(s) | 1458 |
| SmlI | CTYRAG | 4 cut(s) | 365, 1335, 2007, 2031 |
| SmoI | CTYRAG | 4 cut(s) | 365, 1335, 2007, 2031 |
| SnaBI | TACGTA | 1 cut(s) | 1943 |
| SpeI | ACTAGT | 1 cut(s) | 1015 |
| SsiI | CCGC | 2 cut(s) | 1525, 1977 |
| SspMI | CTAG | 5 cut(s) | 315, 1016, 1110, 1133, 1512 |
| SstI | GAGCTC | 1 cut(s) | 550 |
| StyD4I | CCNGG | 6 cut(s) | 661, 910, 1455, 1456, 1690, 1744 |
| StyI | CCWWGG | 2 cut(s) | 149, 592 |
| TaaI | ACNGT | 4 cut(s) | 905, 925, 1139, 1933 |
| TaiI | ACGT | 2 cut(s) | 1927, 1945 |
| TaqI | TCGA | 4 cut(s) | 165, 382, 1201, 1322 |
| TatI | WGTACW | 3 cut(s) | 111, 141, 1533 |
| TfiI | GAWTC | 2 cut(s) | 443, 1666 |
| Tru1I | TTAA | 3 cut(s) | 1437, 1632, 1782 |
| Tru9I | TTAA | 3 cut(s) | 1437, 1632, 1782 |
| TscAI | CASTG | 7 cut(s) | 265, 397, 433, 610, 910, 1035, 1068 |
| TseFI | GTSAC | 5 cut(s) | 120, 388, 1169, 1462, 1498 |
| TseI | GCWGC | 9 cut(s) | 248, 476, 617, 701, 752, 959, 1376, 1475, 1586 |
| Tsp45I | GTSAC | 5 cut(s) | 120, 388, 1169, 1462, 1498 |
| TspDTI | ATGAA | 9 cut(s) | 92, 102, 282, 477, 500, 597, 1030, 1331, 1679 |
| TspGWI | ACGGA | 3 cut(s) | 295, 666, 2041 |
| TspMI | CCCGGG | 1 cut(s) | 1456 |
| TspRI | CASTG | 7 cut(s) | 265, 397, 433, 610, 910, 1035, 1068 |
| Tth111I | GACNNNGTC | 1 cut(s) | 343 |
| VneI | GTGCAC | 1 cut(s) | 1209 |
| VpaK11BI | GGWCC | 3 cut(s) | 153, 908, 1409 |
| XapI | RAATTY | 4 cut(s) | 517, 1419, 1697, 1972 |
| XbaI | TCTAGA | 1 cut(s) | 1511 |
| XmaI | CCCGGG | 1 cut(s) | 1456 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1037 |
| XspI | CTAG | 5 cut(s) | 315, 1016, 1110, 1133, 1512 |
| ZrmI | AGTACT | 1 cut(s) | 143 |