subtilase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 533 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 2117 |
| AatII | GACGTC | 1 cut(s) | 341 |
| Acc36I | ACCTGC | 3 cut(s) | 533, 1282, 1994 |
| Acc65I | GGTACC | 1 cut(s) | 2153 |
| AccB1I | GGYRCC | 1 cut(s) | 2153 |
| AccI | GTMKAC | 3 cut(s) | 105, 780, 1722 |
| AccII | CGCG | 3 cut(s) | 129, 131, 764 |
| AciI | CCGC | 7 cut(s) | 131, 699, 893, 903, 1346, 1734, 1794 |
| AclI | AACGTT | 1 cut(s) | 1952 |
| AclWI | GGATC | 3 cut(s) | 950, 1468, 2020 |
| AcoI | YGGCCR | 6 cut(s) | 390, 437, 586, 864, 1731, 1902 |
| AcyI | GRCGYC | 3 cut(s) | 338, 417, 1104 |
| AdeI | CACNNNGTG | 1 cut(s) | 1124 |
| AfaI | GTAC | 7 cut(s) | 268, 571, 595, 1061, 1535, 1927, 2155 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 723, 1156, 1280 |
| AflIII | ACRYGT | 2 cut(s) | 1525, 1717 |
| AgsI | TTSAA | 8 cut(s) | 170, 232, 809, 1375, 1557, 1634, 1896, 1945 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1303 |
| AhlI | ACTAGT | 1 cut(s) | 581 |
| AjiI | CACGTC | 2 cut(s) | 1526, 1679 |
| AjnI | CCWGG | 3 cut(s) | 245, 1462, 1744 |
| Alw21I | GWGCWC | 1 cut(s) | 1853 |
| Alw26I | GTCTC | 7 cut(s) | 70, 129, 546, 928, 1311, 1318, 1623 |
| AlwI | GGATC | 3 cut(s) | 950, 1468, 2020 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 617 |
| Ama87I | CYCGRG | 1 cut(s) | 1831 |
| AoxI | GGCC | 8 cut(s) | 390, 437, 586, 684, 864, 1658, 1731, 1902 |
| ApeKI | GCWGC | 9 cut(s) | 14, 242, 614, 956, 1085, 1115, 1481, 1586, 2100 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 452, 484 |
| AseI | ATTAAT | 1 cut(s) | 272 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1037 |
| Asp718I | GGTACC | 1 cut(s) | 2153 |
| AspLEI | GCGC | 3 cut(s) | 131, 937, 1129 |
| AspS9I | GGNCC | 4 cut(s) | 473, 905, 1181, 1415 |
| AsuC2I | CCSGG | 1 cut(s) | 1021 |
| AsuHPI | GGTGA | 9 cut(s) | 220, 370, 514, 781, 976, 1256, 1482, 1511, 1847 |
| AsuII | TTCGAA | 2 cut(s) | 825, 1811 |
| AvaI | CYCGRG | 1 cut(s) | 1831 |
| AvaII | GGWCC | 4 cut(s) | 473, 905, 1181, 1415 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 78, 111 |
| BalI | TGGCCA | 3 cut(s) | 392, 588, 866 |
| BanI | GGYRCC | 1 cut(s) | 2153 |
| BauI | CACGAG | 1 cut(s) | 1410 |
| BbsI | GAAGAC | 2 cut(s) | 741, 1820 |
| Bbv12I | GWGCWC | 1 cut(s) | 1853 |
| BbvI | GCAGC | 8 cut(s) | 254, 626, 968, 1097, 1127, 1468, 1573, 2112 |
| BccI | CCATC | 6 cut(s) | 800, 823, 1433, 1608, 1649, 1723 |
| BceAI | ACGGC | 5 cut(s) | 250, 618, 1021, 1128, 1134 |
| BciT130I | CCWGG | 3 cut(s) | 247, 1464, 1746 |
| BcnI | CCSGG | 1 cut(s) | 1021 |
| BcoDI | GTCTC | 7 cut(s) | 70, 129, 546, 928, 1311, 1318, 1623 |
| BcuI | ACTAGT | 1 cut(s) | 581 |
| BfaI | CTAG | 8 cut(s) | 11, 582, 690, 1067, 1242, 1611, 1875, 2040 |
| BfmI | CTRYAG | 1 cut(s) | 1083 |
| BfoI | RGCGCY | 1 cut(s) | 938 |
| BfuAI | ACCTGC | 3 cut(s) | 533, 1282, 1994 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1112 |
| BlpI | GCTNAGC | 1 cut(s) | 2021 |
| BmcAI | AGTACT | 1 cut(s) | 1535 |
| Bme1390I | CCNGG | 4 cut(s) | 247, 1021, 1464, 1746 |
| Bme18I | GGWCC | 4 cut(s) | 473, 905, 1181, 1415 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1303 |
| BmeT110I | CYCGRG | 1 cut(s) | 1831 |
| BmgBI | CACGTC | 2 cut(s) | 1526, 1679 |
| BmgT120I | GGNCC | 4 cut(s) | 473, 905, 1181, 1415 |
| BmiI | GGNNCC | 4 cut(s) | 41, 664, 1918, 2155 |
| BmrFI | CCNGG | 4 cut(s) | 247, 1021, 1464, 1746 |
| BmrI | ACTGGG | 1 cut(s) | 438 |
| BmsI | GCATC | 3 cut(s) | 1692, 1697, 2034 |
| BmuI | ACTGGG | 1 cut(s) | 438 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1961 |
| BpiI | GAAGAC | 2 cut(s) | 741, 1820 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1671 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2021 |
| Bpu14I | TTCGAA | 2 cut(s) | 825, 1811 |
| BpuEI | CTTGAG | 1 cut(s) | 1427 |
| BpuMI | CCSGG | 1 cut(s) | 1021 |
| BsaBI | GATNNNNATC | 1 cut(s) | 842 |
| BsaHI | GRCGYC | 3 cut(s) | 338, 417, 1104 |
| BsaI | GGTCTC | 1 cut(s) | 1623 |
| BsaJI | CCNNGG | 3 cut(s) | 589, 938, 973 |
| BsaWI | WCCGGW | 1 cut(s) | 506 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 723, 1156, 1280 |
| Bse1I | ACTGG | 1 cut(s) | 433 |
| Bse3DI | GCAATG | 1 cut(s) | 664 |
| Bse8I | GATNNNNATC | 1 cut(s) | 842 |
| BseBI | CCWGG | 3 cut(s) | 247, 1464, 1746 |
| BseDI | CCNNGG | 3 cut(s) | 589, 938, 973 |
| BseGI | GGATG | 1 cut(s) | 815 |
| BseJI | GATNNNNATC | 1 cut(s) | 842 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 723, 1156, 1280 |
| BseMI | GCAATG | 1 cut(s) | 664 |
| BseMII | CTCAG | 2 cut(s) | 81, 246 |
| BseNI | ACTGG | 1 cut(s) | 433 |
| BseRI | GAGGAG | 2 cut(s) | 238, 468 |
| BseXI | GCAGC | 8 cut(s) | 254, 626, 968, 1097, 1127, 1468, 1573, 2112 |
| BsgI | GTGCAG | 1 cut(s) | 697 |
| Bsh1236I | CGCG | 3 cut(s) | 129, 131, 764 |
| Bsh1285I | CGRYCG | 3 cut(s) | 195, 846, 1695 |
| BshFI | GGCC | 8 cut(s) | 392, 439, 588, 686, 866, 1660, 1733, 1904 |
| BshNI | GGYRCC | 1 cut(s) | 2153 |
| BsiEI | CGRYCG | 3 cut(s) | 195, 846, 1695 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1853 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1831 |
| BsiSI | CCGG | 5 cut(s) | 440, 507, 683, 1020, 1448 |
| BslFI | GGGAC | 6 cut(s) | 359, 590, 1312, 1689, 2105, 2157 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 723, 1156, 1280 |
| BsmAI | GTCTC | 7 cut(s) | 70, 129, 546, 928, 1311, 1318, 1623 |
| BsmBI | CGTCTC | 2 cut(s) | 129, 1318 |
| BsmFI | GGGAC | 6 cut(s) | 359, 590, 1312, 1689, 2105, 2157 |
| BsmI | GAATGC | 2 cut(s) | 1039, 1256 |
| BsnI | GGCC | 8 cut(s) | 392, 439, 588, 686, 866, 1660, 1733, 1904 |
| Bso31I | GGTCTC | 1 cut(s) | 1623 |
| BsoBI | CYCGRG | 1 cut(s) | 1831 |
| Bsp119I | TTCGAA | 2 cut(s) | 825, 1811 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1853 |
| Bsp143I | GATC | 6 cut(s) | 151, 192, 843, 942, 1473, 2012 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2021 |
| Bsp19I | CCATGG | 1 cut(s) | 938 |
| BspACI | CCGC | 7 cut(s) | 131, 699, 893, 903, 1346, 1734, 1794 |
| BspANI | GGCC | 8 cut(s) | 392, 439, 588, 686, 866, 1660, 1733, 1904 |
| BspCNI | CTCAG | 2 cut(s) | 80, 247 |
| BspFNI | CGCG | 3 cut(s) | 129, 131, 764 |
| BspHI | TCATGA | 1 cut(s) | 1644 |
| BspLI | GGNNCC | 4 cut(s) | 41, 664, 1918, 2155 |
| BspMAI | CTGCAG | 1 cut(s) | 1087 |
| BspMI | ACCTGC | 3 cut(s) | 533, 1282, 1994 |
| BspPI | GGATC | 3 cut(s) | 950, 1468, 2020 |
| BspT104I | TTCGAA | 2 cut(s) | 825, 1811 |
| BspT107I | GGYRCC | 1 cut(s) | 2153 |
| BspTNI | GGTCTC | 1 cut(s) | 1623 |
| BsrDI | GCAATG | 1 cut(s) | 664 |
| BsrI | ACTGG | 1 cut(s) | 433 |
| BssECI | CCNNGG | 3 cut(s) | 589, 938, 973 |
| BssMI | GATC | 6 cut(s) | 151, 192, 843, 942, 1473, 2012 |
| BssNI | GRCGYC | 3 cut(s) | 338, 417, 1104 |
| BssSI | CACGAG | 1 cut(s) | 1410 |
| BssT1I | CCWWGG | 1 cut(s) | 938 |
| Bst2BI | CACGAG | 1 cut(s) | 1410 |
| Bst2UI | CCWGG | 3 cut(s) | 247, 1464, 1746 |
| Bst6I | CTCTTC | 1 cut(s) | 1411 |
| BstACI | GRCGYC | 3 cut(s) | 338, 417, 1104 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1124 |
| BstBI | TTCGAA | 2 cut(s) | 825, 1811 |
| BstC8I | GCNNGC | 3 cut(s) | 961, 1131, 1970 |
| BstDEI | CTNAG | 6 cut(s) | 67, 255, 483, 1671, 2016, 2021 |
| BstDSI | CCRYGG | 3 cut(s) | 589, 938, 973 |
| BstF5I | GGATG | 1 cut(s) | 815 |
| BstFNI | CGCG | 3 cut(s) | 129, 131, 764 |
| BstH2I | RGCGCY | 1 cut(s) | 938 |
| BstHHI | GCGC | 3 cut(s) | 131, 937, 1129 |
| BstKTI | GATC | 6 cut(s) | 154, 195, 846, 945, 1476, 2015 |
| BstMAI | GTCTC | 7 cut(s) | 70, 129, 546, 928, 1311, 1318, 1623 |
| BstMBI | GATC | 6 cut(s) | 151, 192, 843, 942, 1473, 2012 |
| BstMCI | CGRYCG | 3 cut(s) | 195, 846, 1695 |
| BstNI | CCWGG | 3 cut(s) | 247, 1464, 1746 |
| BstNSI | RCATGY | 2 cut(s) | 932, 1721 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1961 |
| BstSCI | CCNGG | 4 cut(s) | 245, 1019, 1462, 1744 |
| BstSFI | CTRYAG | 1 cut(s) | 1083 |
| BstUI | CGCG | 3 cut(s) | 129, 131, 764 |
| BstV1I | GCAGC | 8 cut(s) | 254, 626, 968, 1097, 1127, 1468, 1573, 2112 |
| BstV2I | GAAGAC | 2 cut(s) | 741, 1820 |
| BstX2I | RGATCY | 3 cut(s) | 942, 1473, 2012 |
| BstYI | RGATCY | 3 cut(s) | 942, 1473, 2012 |
| BsuRI | GGCC | 8 cut(s) | 392, 439, 588, 686, 866, 1660, 1733, 1904 |
| BtgI | CCRYGG | 3 cut(s) | 589, 938, 973 |
| BtgZI | GCGATG | 3 cut(s) | 1411, 1669, 1765 |
| BtrI | CACGTC | 2 cut(s) | 1526, 1679 |
| BtsCI | GGATG | 1 cut(s) | 815 |
| BtsIMutI | CAGTG | 6 cut(s) | 252, 426, 879, 1035, 2067, 2088 |
| BveI | ACCTGC | 3 cut(s) | 533, 1282, 1994 |
| Cac8I | GCNNGC | 3 cut(s) | 961, 1131, 1970 |
| CaiI | CAGNNNCTG | 1 cut(s) | 617 |
| CciI | TCATGA | 1 cut(s) | 1644 |
| CfoI | GCGC | 3 cut(s) | 131, 937, 1129 |
| Cfr13I | GGNCC | 4 cut(s) | 473, 905, 1181, 1415 |
| CseI | GACGC | 4 cut(s) | 6, 406, 770, 1112 |
| Csp6I | GTAC | 7 cut(s) | 267, 570, 594, 1060, 1534, 1926, 2154 |
| CviQI | GTAC | 7 cut(s) | 267, 570, 594, 1060, 1534, 1926, 2154 |
| DdeI | CTNAG | 6 cut(s) | 67, 255, 483, 1671, 2016, 2021 |
| DpnI | GATC | 6 cut(s) | 153, 194, 845, 944, 1475, 2014 |
| DpnII | GATC | 6 cut(s) | 151, 192, 843, 942, 1473, 2012 |
| DraI | TTTAAA | 2 cut(s) | 1287, 1740 |
| DraIII | CACNNNGTG | 1 cut(s) | 1124 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2117 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1303 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2117 |
| EaeI | YGGCCR | 6 cut(s) | 390, 437, 586, 864, 1731, 1902 |
| Eam1104I | CTCTTC | 1 cut(s) | 1411 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1303 |
| EarI | CTCTTC | 1 cut(s) | 1411 |
| Eco130I | CCWWGG | 1 cut(s) | 938 |
| Eco31I | GGTCTC | 1 cut(s) | 1623 |
| Eco47I | GGWCC | 4 cut(s) | 473, 905, 1181, 1415 |
| Eco88I | CYCGRG | 1 cut(s) | 1831 |
| EcoRII | CCWGG | 3 cut(s) | 245, 1462, 1744 |
| EcoT14I | CCWWGG | 1 cut(s) | 938 |
| ErhI | CCWWGG | 1 cut(s) | 938 |
| Esp3I | CGTCTC | 2 cut(s) | 129, 1318 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 371, 403 |
| FaqI | GGGAC | 6 cut(s) | 359, 590, 1312, 1689, 2105, 2157 |
| FauI | CCCGC | 1 cut(s) | 886 |
| FblI | GTMKAC | 3 cut(s) | 105, 780, 1722 |
| FokI | GGATG | 1 cut(s) | 802 |
| FspBI | CTAG | 8 cut(s) | 11, 582, 690, 1067, 1242, 1611, 1875, 2040 |
| GlaI | GCGC | 3 cut(s) | 130, 936, 1128 |
| HaeII | RGCGCY | 1 cut(s) | 938 |
| HaeIII | GGCC | 8 cut(s) | 392, 439, 588, 686, 866, 1660, 1733, 1904 |
| HapII | CCGG | 5 cut(s) | 440, 507, 683, 1020, 1448 |
| HgaI | GACGC | 4 cut(s) | 6, 406, 770, 1112 |
| HhaI | GCGC | 3 cut(s) | 131, 937, 1129 |
| Hin1I | GRCGYC | 3 cut(s) | 338, 417, 1104 |
| Hin6I | GCGC | 3 cut(s) | 129, 935, 1127 |
| HinP1I | GCGC | 3 cut(s) | 129, 935, 1127 |
| HincII | GTYRAC | 3 cut(s) | 1723, 1956, 2110 |
| HindII | GTYRAC | 3 cut(s) | 1723, 1956, 2110 |
| HindIII | AAGCTT | 1 cut(s) | 2018 |
| HpaII | CCGG | 5 cut(s) | 440, 507, 683, 1020, 1448 |
| HphI | GGTGA | 9 cut(s) | 220, 370, 514, 781, 976, 1256, 1482, 1511, 1847 |
| Hpy188III | TCNNGA | 5 cut(s) | 690, 1548, 1597, 1645, 1914 |
| Hpy99I | CGWCG | 5 cut(s) | 337, 340, 1253, 1967, 2108 |
| HpyF3I | CTNAG | 6 cut(s) | 67, 255, 483, 1671, 2016, 2021 |
| Hsp92I | GRCGYC | 3 cut(s) | 338, 417, 1104 |
| HspAI | GCGC | 3 cut(s) | 129, 935, 1127 |
| KpnI | GGTACC | 1 cut(s) | 2157 |
| Kzo9I | GATC | 6 cut(s) | 151, 192, 843, 942, 1473, 2012 |
| LmnI | GCTCC | 5 cut(s) | 45, 848, 1214, 1709, 1856 |
| Lsp1109I | GCAGC | 8 cut(s) | 254, 626, 968, 1097, 1127, 1468, 1573, 2112 |
| LweI | GCATC | 3 cut(s) | 1692, 1697, 2034 |
| MaeI | CTAG | 8 cut(s) | 11, 582, 690, 1067, 1242, 1611, 1875, 2040 |
| MalI | GATC | 6 cut(s) | 153, 194, 845, 944, 1475, 2014 |
| MboI | GATC | 6 cut(s) | 151, 192, 843, 942, 1473, 2012 |
| MboII | GAAGA | 8 cut(s) | 95, 158, 741, 839, 1363, 1398, 1523, 1825 |
| MflI | RGATCY | 3 cut(s) | 942, 1473, 2012 |
| MhlI | GDGCHC | 1 cut(s) | 1853 |
| MlsI | TGGCCA | 3 cut(s) | 392, 588, 866 |
| MluCI | AATT | 5 cut(s) | 273, 809, 1760, 1907, 2139 |
| MluNI | TGGCCA | 3 cut(s) | 392, 588, 866 |
| MlyI | GAGTC | 3 cut(s) | 266, 1334, 2105 |
| MmeI | TCCRAC | 2 cut(s) | 483, 1192 |
| Mox20I | TGGCCA | 3 cut(s) | 392, 588, 866 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1037 |
| MscI | TGGCCA | 3 cut(s) | 392, 588, 866 |
| MseI | TTAA | 7 cut(s) | 272, 1286, 1302, 1739, 1821, 1884, 2055 |
| MslI | CAYNNNNRTG | 1 cut(s) | 356 |
| Msp20I | TGGCCA | 3 cut(s) | 392, 588, 866 |
| MspA1I | CMGCKG | 2 cut(s) | 617, 903 |
| MspI | CCGG | 5 cut(s) | 440, 507, 683, 1020, 1448 |
| MspR9I | CCNGG | 4 cut(s) | 247, 1021, 1464, 1746 |
| Mva1269I | GAATGC | 2 cut(s) | 1039, 1256 |
| MvaI | CCWGG | 3 cut(s) | 247, 1464, 1746 |
| MvnI | CGCG | 3 cut(s) | 129, 131, 764 |
| NciI | CCSGG | 1 cut(s) | 1021 |
| NcoI | CCATGG | 1 cut(s) | 938 |
| NdeII | GATC | 6 cut(s) | 151, 192, 843, 942, 1473, 2012 |
| NlaIV | GGNNCC | 4 cut(s) | 41, 664, 1918, 2155 |
| NmuCI | GTSAC | 6 cut(s) | 175, 1100, 1936, 2006, 2032, 2086 |
| NspI | RCATGY | 2 cut(s) | 932, 1721 |
| NspV | TTCGAA | 2 cut(s) | 825, 1811 |
| PagI | TCATGA | 1 cut(s) | 1644 |
| PaqCI | CACCTGC | 1 cut(s) | 533 |
| PciI | ACATGT | 1 cut(s) | 1717 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 420, 1692 |
| PctI | GAATGC | 2 cut(s) | 1039, 1256 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1037 |
| PfeI | GAWTC | 8 cut(s) | 7, 443, 651, 693, 719, 874, 1334, 1630 |
| PflFI | GACNNNGTC | 1 cut(s) | 337 |
| PfoI | TCCNGGA | 1 cut(s) | 1744 |
| Ple19I | CGATCG | 2 cut(s) | 195, 846 |
| PleI | GAGTC | 3 cut(s) | 265, 1333, 2105 |
| PpsI | GAGTC | 3 cut(s) | 265, 1333, 2105 |
| PscI | ACATGT | 1 cut(s) | 1717 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1961 |
| PshBI | ATTAAT | 1 cut(s) | 272 |
| Psp1406I | AACGTT | 1 cut(s) | 1952 |
| Psp6I | CCWGG | 3 cut(s) | 245, 1462, 1744 |
| PspGI | CCWGG | 3 cut(s) | 245, 1462, 1744 |
| PspN4I | GGNNCC | 4 cut(s) | 41, 664, 1918, 2155 |
| PspPI | GGNCC | 4 cut(s) | 473, 905, 1181, 1415 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1909, 1941 |
| PstI | CTGCAG | 1 cut(s) | 1087 |
| PstNI | CAGNNNCTG | 1 cut(s) | 617 |
| PsuI | RGATCY | 3 cut(s) | 942, 1473, 2012 |
| PsyI | GACNNNGTC | 1 cut(s) | 337 |
| PvuI | CGATCG | 2 cut(s) | 195, 846 |
| PvuII | CAGCTG | 1 cut(s) | 617 |
| RsaI | GTAC | 7 cut(s) | 268, 571, 595, 1061, 1535, 1927, 2155 |
| RsaNI | GTAC | 7 cut(s) | 267, 570, 594, 1060, 1534, 1926, 2154 |
| RseI | CAYNNNNRTG | 1 cut(s) | 356 |
| SalI | GTCGAC | 1 cut(s) | 1721 |
| SaqAI | TTAA | 7 cut(s) | 272, 1286, 1302, 1739, 1821, 1884, 2055 |
| Sau3AI | GATC | 6 cut(s) | 151, 192, 843, 942, 1473, 2012 |
| Sau96I | GGNCC | 4 cut(s) | 473, 905, 1181, 1415 |
| ScaI | AGTACT | 1 cut(s) | 1535 |
| SchI | GAGTC | 3 cut(s) | 266, 1334, 2105 |
| ScrFI | CCNGG | 4 cut(s) | 247, 1021, 1464, 1746 |
| SduI | GDGCHC | 1 cut(s) | 1853 |
| SfaNI | GCATC | 3 cut(s) | 1692, 1697, 2034 |
| SfcI | CTRYAG | 1 cut(s) | 1083 |
| SfuI | TTCGAA | 2 cut(s) | 825, 1811 |
| SinI | GGWCC | 4 cut(s) | 473, 905, 1181, 1415 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 356 |
| SmlI | CTYRAG | 1 cut(s) | 1442 |
| SmoI | CTYRAG | 1 cut(s) | 1442 |
| SpeI | ACTAGT | 1 cut(s) | 581 |
| Sse9I | AATT | 5 cut(s) | 273, 809, 1760, 1907, 2139 |
| SsiI | CCGC | 7 cut(s) | 131, 699, 893, 903, 1346, 1734, 1794 |
| SspMI | CTAG | 8 cut(s) | 11, 582, 690, 1067, 1242, 1611, 1875, 2040 |
| StyD4I | CCNGG | 4 cut(s) | 245, 1019, 1462, 1744 |
| StyI | CCWWGG | 1 cut(s) | 938 |
| TaqI | TCGA | 9 cut(s) | 191, 195, 423, 756, 825, 1041, 1691, 1722, 1811 |
| TaqII | GACCGA | 1 cut(s) | 1709 |
| TasI | AATT | 5 cut(s) | 273, 809, 1760, 1907, 2139 |
| TatI | WGTACW | 1 cut(s) | 1533 |
| TauI | GCSGC | 4 cut(s) | 701, 1349, 1736, 1797 |
| TfiI | GAWTC | 8 cut(s) | 7, 443, 651, 693, 719, 874, 1334, 1630 |
| Tru1I | TTAA | 7 cut(s) | 272, 1286, 1302, 1739, 1821, 1884, 2055 |
| Tru9I | TTAA | 7 cut(s) | 272, 1286, 1302, 1739, 1821, 1884, 2055 |
| TscAI | CASTG | 6 cut(s) | 259, 433, 886, 1035, 2067, 2095 |
| TseFI | GTSAC | 6 cut(s) | 175, 1100, 1936, 2006, 2032, 2086 |
| TseI | GCWGC | 9 cut(s) | 14, 242, 614, 956, 1085, 1115, 1481, 1586, 2100 |
| Tsp45I | GTSAC | 6 cut(s) | 175, 1100, 1936, 2006, 2032, 2086 |
| TspDTI | ATGAA | 6 cut(s) | 96, 340, 1061, 1080, 1773, 1814 |
| TspGWI | ACGGA | 2 cut(s) | 1376, 2025 |
| TspRI | CASTG | 6 cut(s) | 259, 433, 886, 1035, 2067, 2095 |
| Tth111I | GACNNNGTC | 1 cut(s) | 337 |
| VpaK11BI | GGWCC | 4 cut(s) | 473, 905, 1181, 1415 |
| VspI | ATTAAT | 1 cut(s) | 272 |
| XbaI | TCTAGA | 1 cut(s) | 689 |
| XceI | RCATGY | 2 cut(s) | 932, 1721 |
| XmiI | GTMKAC | 3 cut(s) | 105, 780, 1722 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1037 |
| XspI | CTAG | 8 cut(s) | 11, 582, 690, 1067, 1242, 1611, 1875, 2040 |
| ZraI | GACGTC | 1 cut(s) | 339 |
| ZrmI | AGTACT | 1 cut(s) | 1535 |