Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000257

Physical Location & Seq

Genomic Coordinates Forward (+)

23759003 .. 23762438

Gene Structure 3436 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G58840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2142 bp

ATGGCGAGACGAGCAGATTCGTTTTGTCTCATATCATGTGTTCTTGTCTCGTTTGTAATTTCAGTCTCTGCAGTGACAGATGATTCTCAAGATAAACAAGTGTATGTTGTCTACATGGGCTCACTTCCGTCGTCTCGACTGGAATACACACCAATGTCGCATCATATGAGTATTCTTCAAGAGGTCACCGGAGAAAGTTCGGTGGAAGGTCGTTTGGTGAGAAGTTACAAGAGGAGTTTCAACGGTTTTGCGGCCCGACTCACTGAGTCAGAACGAGAAAGAGTAGCCGAAATGGAGGGAGTTGTGTCTGTGTTTCCGGACATTAACTACAAACTCCAAACGACGGCGTCTTGGGATTTCTTGGGGTTGAAGGAAGGAAAAAATACAAAGCGAAACTTGGCCATAGAAAGTGATACTATTATCGGATTCATCGACAGTGGGATTTGGCCAGAATCCGAGAGCTTTTCTGATAAAGGCTTTGGTCCTCCTCCTAAGAAATGGAAAGGTGTTTGTTCCGCCGGCAAAAACTTCACTTGTAACAACAAGTTGATTGGAGCAAGAGACTATACAAACGAAGGCACCAGGGACATTGAAGGCCACGGTACACACACAGCCTCAACAGCGGCTGGAAACGCAGTTAAGAACACAAGTTTCTACGGAATCGGTAATGGAACTGCGAGAGGTGGCGTTCCAGCCTCAAGAATCGCAGCTTACAAAGCCTGTAGCGAGATGGGGTGTACCACTGAATCTGTACTATCGGCGTTCGATGATGCGATAGCAGACGGTGTTGACCTCATCAGCATCTCTCTCGGGGCAAACTTAGTCCGCACGTACGAAACGGACCCGATAGCGATTGGGGCTTTTCACGCTATGGTCAAAGGTATTCTCACTGTGCAGTCAGCTGGTAACGGTGGTCCGAATCCGGGCTCAGTCATGAGTGTAGCACCATGGATCTTGACAGTTGCAGCAAGCAACACCAACCGTGGGTTTGTCACCAAAGTTGTACTCGGAAACGGCAAGACATTTGTCGGGAAATCATTGAACGCTTTTGATTTAAAAGGAAAGAATTATCCTCTGTATGGAGGTTCTACTGATGGACCTCTGCTTAGAGGGAAAATCTTGGTGAGTGAAGATAAAGTTAGTTCAGAAATCGTTGTTGCAAACATAAATGAAAATTACCATGACTATGCCTACGTTAGCATTCTGCCCTCCTCTGCTCTGTCAAAAGATGACTTTGACTCTGTCATCTCTTACGTTAACTCCACGAAGTCCCCGCACGGGACTGTTCTAAAAAGTGAAGCAATCTTTAACCAGGCAGCTCCTAAAGTTGCTGGATTCTCTTCTCGCGGTCCAAACACTATTGCGGTTGATATACTTAAGCCAGATGTAACAGCACCAGGAGTGGAGATTCTCGCTGCGTTTTCACCTTTGAATTCACCAGCTCAAGACAAAAGAGATAATAGACATGTAAAGTACTCTGTTCTGTCGGGAACTTCAATGTCTTGTCCACACGTTGCAGGCGTGGCTGCGTACATCAAGACGTTTCATCCTGAATGGTCTCCTTCCATGATCCAATCTGCCATCATGACAACTGCTTGGCCGATGAATGCTACCGGAACTGCGGTTGCATCAACCGAGTTTGCTTATGGAGCTGGACATGTTGACCCAATAGCAGCTATAAATCCAGGTCTCGTTTATGAAATAGGCAAATCAGACCACATCGCCTTTCTCTGTGGTTTGAACTACAATGCGACTTCTCTAAAACTCATCGCTGGTGAAGCTGTCACTTGCACTGGCAAGACCTTACCAAGAAACCTAAACTACCCTTCAATGTCAGCTAAATTGCCGAAATCTGAAAGCTCCTTCATCGTGACTTTCAACAGAACCGTAACCAACGTAGGTACCCCAAACTCAACATACAAATCAAAGATTGTCTTAAATCACGGATCTAACCTCAAAGTCGAGGTCTCCCCAAGTGTTCTATCTATGAAGTCAGTGAAGGAGAAGCAGTCTTTTACAGTGACTGTTTCAGGGAGCAATATCGACCCAAAACTGCCTTCTTCTGCAAATCTAATATGGTCTGATGGAACCCATAACGTGAGAAGCCCCATTGTTGTTTATACTTATAGTGTTAGTGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009719 GO:0009725 GO:0009753 GO:0010033 GO:0010035 GO:0010038 GO:0016787 GO:0017171 GO:0019538 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044464 GO:0046686 GO:0050896 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

713

Amino Acids

75.96

Weight (kDa)

8.18

Isoelectric Point (pI)

37.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	34 - 113	2.1e-19	Peptidase inhibitor I9
Peptidase_S8	PF00082	137 - 552	1.2e-43	Subtilase family
fn3_6	PF17766	605 - 706	3.1e-25	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca	FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica	MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica	Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis	pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis	RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata	RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora	Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii	Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa	Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha	Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana	Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1901
AccB1I	GGYRCC	2 cut(s)	578, 1901
AccI	GTMKAC	1 cut(s)	111
AccII	CGCG	1 cut(s)	1347
AccIII	TCCGGA	1 cut(s)	316
AciI	CCGC	8 cut(s)	251, 516, 623, 826, 1274, 1347, 1364, 1622
AclWI	GGATC	3 cut(s)	959, 1564, 1954
AcoI	YGGCCR	3 cut(s)	399, 446, 1598
AcsI	RAATTY	1 cut(s)	1432
AcyI	GRCGYC	1 cut(s)	347
AfaI	GTAC	8 cut(s)	604, 739, 753, 833, 1005, 1475, 1532, 1903
AfiI	CCNNNNNNNGG	5 cut(s)	343, 923, 984, 1079, 1278
AflII	CTTAAG	1 cut(s)	1376
AflIII	ACRYGT	3 cut(s)	1465, 1510, 1657
AjnI	CCWGG	4 cut(s)	581, 1311, 1396, 1684
AjuI	GAANNNNNNNTTGG	2 cut(s)	2041, 2073
Alw26I	GTCTC	8 cut(s)	32, 52, 70, 138, 555, 1563, 1694, 1972
AlwI	GGATC	3 cut(s)	959, 1564, 1954
AlwNI	CAGNNNCTG	3 cut(s)	68, 626, 2024
Ama87I	CYCGRG	1 cut(s)	809
Aor13HI	TCCGGA	1 cut(s)	316
AoxI	GGCC	5 cut(s)	252, 399, 446, 595, 1598
ApeKI	GCWGC	6 cut(s)	707, 965, 1316, 1415, 1526, 1673
ApoI	RAATTY	1 cut(s)	1432
ArsI	GACNNNNNNTTYG	2 cut(s)	1950, 1982
Asp718I	GGTACC	1 cut(s)	1901
AspS9I	GGNCC	6 cut(s)	253, 482, 841, 914, 1097, 1349
AsuC2I	CCSGG	1 cut(s)	924
AsuHPI	GGTGA	7 cut(s)	178, 229, 985, 1135, 1416, 1428, 1787
AvaI	CYCGRG	1 cut(s)	809
AvaII	GGWCC	5 cut(s)	482, 841, 914, 1097, 1349
BaeI	ACNNNNGTAYC	2 cut(s)	1885, 1918
BalI	TGGCCA	2 cut(s)	401, 448
BanI	GGYRCC	2 cut(s)	578, 1901
BanII	GRGCYC	2 cut(s)	122, 929
BbvI	GCAGC	6 cut(s)	719, 977, 1328, 1402, 1513, 1685
BccI	CCATC	4 cut(s)	724, 1088, 1589, 2078
BceAI	ACGGC	2 cut(s)	360, 1030
BcgI	CGANNNNNNTGC	2 cut(s)	790, 824
BciT130I	CCWGG	4 cut(s)	583, 1313, 1398, 1686
BcnI	CCSGG	1 cut(s)	924
BcoDI	GTCTC	8 cut(s)	32, 52, 70, 138, 555, 1563, 1694, 1972
BfmI	CTRYAG	2 cut(s)	69, 721
BfrI	CTTAAG	1 cut(s)	1376
BisI	GCNGC	8 cut(s)	252, 624, 708, 966, 1317, 1416, 1527, 1674
BlsI	GCNGC	8 cut(s)	253, 625, 709, 967, 1318, 1417, 1528, 1675
BmcAI	AGTACT	1 cut(s)	1475
Bme1390I	CCNGG	5 cut(s)	583, 924, 1313, 1398, 1686
Bme18I	GGWCC	5 cut(s)	482, 841, 914, 1097, 1349
BmeT110I	CYCGRG	1 cut(s)	809
BmgT120I	GGNCC	6 cut(s)	253, 482, 841, 914, 1097, 1349
BmiI	GGNNCC	4 cut(s)	580, 843, 1903, 2089
BmrFI	CCNGG	5 cut(s)	583, 924, 1313, 1398, 1686
BmsI	GCATC	4 cut(s)	169, 760, 810, 1637
BoxI	GACNNNNGTC	1 cut(s)	1025
BpuEI	CTTGAG	3 cut(s)	72, 682, 1428
BpuMI	CCSGG	1 cut(s)	924
BsaAI	YACGTR	1 cut(s)	831
BsaHI	GRCGYC	1 cut(s)	347
BsaI	GGTCTC	3 cut(s)	1563, 1694, 1972
BsaJI	CCNNGG	4 cut(s)	582, 598, 947, 982
BsaWI	WCCGGW	3 cut(s)	188, 316, 1613
BsaXI	ACNNNNNCTCC	4 cut(s)	1244, 1274, 1994, 2024
Bsc4I	CCNNNNNNNGG	5 cut(s)	343, 923, 984, 1079, 1278
Bse118I	RCCGGY	1 cut(s)	518
Bse1I	ACTGG	2 cut(s)	144, 1798
BseAI	TCCGGA	1 cut(s)	316
BseBI	CCWGG	4 cut(s)	583, 1313, 1398, 1686
BseDI	CCNNGG	4 cut(s)	582, 598, 947, 982
BseGI	GGATG	1 cut(s)	1546
BseLI	CCNNNNNNNGG	5 cut(s)	343, 923, 984, 1079, 1278
BseMII	CTCAG	2 cut(s)	255, 942
BseNI	ACTGG	2 cut(s)	144, 1798
BseRI	GAGGAG	3 cut(s)	247, 477, 1201
BseXI	GCAGC	6 cut(s)	719, 977, 1328, 1402, 1513, 1685
BsgI	GTGCAG	1 cut(s)	914
Bsh1236I	CGCG	1 cut(s)	1347
BshFI	GGCC	5 cut(s)	254, 401, 448, 597, 1600
BshNI	GGYRCC	2 cut(s)	578, 1901
BsiHKCI	CYCGRG	1 cut(s)	809
BsiSI	CCGG	5 cut(s)	189, 317, 519, 923, 1614
BsiWI	CGTACG	1 cut(s)	831
BslFI	GGGAC	3 cut(s)	599, 1255, 1294
BslI	CCNNNNNNNGG	5 cut(s)	343, 923, 984, 1079, 1278
BsmAI	GTCTC	8 cut(s)	32, 52, 70, 138, 555, 1563, 1694, 1972
BsmBI	CGTCTC	1 cut(s)	138
BsmFI	GGGAC	3 cut(s)	599, 1255, 1294
BsmI	GAATGC	2 cut(s)	1200, 1612
BsnI	GGCC	5 cut(s)	254, 401, 448, 597, 1600
Bso31I	GGTCTC	3 cut(s)	1563, 1694, 1972
BsoBI	CYCGRG	1 cut(s)	809
Bsp1286I	GDGCHC	2 cut(s)	122, 929
Bsp13I	TCCGGA	1 cut(s)	316
Bsp143I	GATC	3 cut(s)	951, 1569, 1946
Bsp19I	CCATGG	1 cut(s)	947
BspACI	CCGC	8 cut(s)	251, 516, 623, 826, 1274, 1347, 1364, 1622
BspANI	GGCC	5 cut(s)	254, 401, 448, 597, 1600
BspCNI	CTCAG	2 cut(s)	256, 941
BspEI	TCCGGA	1 cut(s)	316
BspFNI	CGCG	1 cut(s)	1347
BspHI	TCATGA	2 cut(s)	933, 1584
BspLI	GGNNCC	4 cut(s)	580, 843, 1903, 2089
BspMAI	CTGCAG	1 cut(s)	73
BspPI	GGATC	3 cut(s)	959, 1564, 1954
BspT107I	GGYRCC	2 cut(s)	578, 1901
BspTI	CTTAAG	1 cut(s)	1376
BspTNI	GGTCTC	3 cut(s)	1563, 1694, 1972
BsrFI	RCCGGY	1 cut(s)	518
BsrI	ACTGG	2 cut(s)	144, 1798
BssAI	RCCGGY	1 cut(s)	518
BssECI	CCNNGG	4 cut(s)	582, 598, 947, 982
BssMI	GATC	3 cut(s)	951, 1569, 1946
BssNI	GRCGYC	1 cut(s)	347
BssT1I	CCWWGG	1 cut(s)	947
Bst2UI	CCWGG	4 cut(s)	583, 1313, 1398, 1686
Bst6I	CTCTTC	1 cut(s)	1345
BstACI	GRCGYC	1 cut(s)	347
BstAFI	CTTAAG	1 cut(s)	1376
BstBAI	YACGTR	1 cut(s)	831
BstC8I	GCNNGC	3 cut(s)	520, 970, 1519
BstDEI	CTNAG	5 cut(s)	264, 492, 820, 928, 1106
BstDSI	CCRYGG	3 cut(s)	598, 947, 982
BstEII	GGTNACC	1 cut(s)	184
BstF5I	GGATG	1 cut(s)	1546
BstFNI	CGCG	1 cut(s)	1347
BstKTI	GATC	3 cut(s)	954, 1572, 1949
BstMAI	GTCTC	8 cut(s)	32, 52, 70, 138, 555, 1563, 1694, 1972
BstMBI	GATC	3 cut(s)	951, 1569, 1946
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 620, 632, 716, 857, 866, 1523, 1649, 1778
BstNI	CCWGG	4 cut(s)	583, 1313, 1398, 1686
BstNSI	RCATGY	2 cut(s)	1469, 1661
BstPAI	GACNNNNGTC	1 cut(s)	1025
BstPI	GGTNACC	1 cut(s)	184
BstSCI	CCNGG	5 cut(s)	581, 922, 1311, 1396, 1684
BstSFI	CTRYAG	2 cut(s)	69, 721
BstUI	CGCG	1 cut(s)	1347
BstV1I	GCAGC	6 cut(s)	719, 977, 1328, 1402, 1513, 1685
BstX2I	RGATCY	2 cut(s)	951, 1946
BstYI	RGATCY	2 cut(s)	951, 1946
BsuRI	GGCC	5 cut(s)	254, 401, 448, 597, 1600
BtgI	CCRYGG	3 cut(s)	598, 947, 982
BtgZI	GCGATG	2 cut(s)	1705, 1753
BtsCI	GGATG	1 cut(s)	1546
BtsI	GCAGTG	1 cut(s)	78
BtsIMutI	CAGTG	8 cut(s)	78, 261, 442, 741, 888, 1791, 2001, 2025
Cac8I	GCNNGC	3 cut(s)	520, 970, 1519
CaiI	CAGNNNCTG	3 cut(s)	68, 626, 2024
CciI	TCATGA	2 cut(s)	933, 1584
Cfr10I	RCCGGY	1 cut(s)	518
Cfr13I	GGNCC	6 cut(s)	253, 482, 841, 914, 1097, 1349
CseI	GACGC	1 cut(s)	336
Csp6I	GTAC	8 cut(s)	603, 738, 752, 832, 1004, 1474, 1531, 1902
CviAII	CATG	9 cut(s)	36, 115, 934, 948, 1181, 1466, 1567, 1585, 1658
CviQI	GTAC	8 cut(s)	603, 738, 752, 832, 1004, 1474, 1531, 1902
DdeI	CTNAG	5 cut(s)	264, 492, 820, 928, 1106
DpnI	GATC	3 cut(s)	953, 1571, 1948
DpnII	GATC	3 cut(s)	951, 1569, 1946
DraI	TTTAAA	1 cut(s)	1056
EaeI	YGGCCR	3 cut(s)	399, 446, 1598
Eam1104I	CTCTTC	1 cut(s)	1345
EarI	CTCTTC	1 cut(s)	1345
EciI	GGCGGA	1 cut(s)	505
Eco130I	CCWWGG	1 cut(s)	947
Eco24I	GRGCYC	2 cut(s)	122, 929
Eco31I	GGTCTC	3 cut(s)	1563, 1694, 1972
Eco47I	GGWCC	5 cut(s)	482, 841, 914, 1097, 1349
Eco88I	CYCGRG	1 cut(s)	809
Eco91I	GGTNACC	1 cut(s)	184
EcoO65I	GGTNACC	1 cut(s)	184
EcoRI	GAATTC	1 cut(s)	1432
EcoRII	CCWGG	4 cut(s)	581, 1311, 1396, 1684
EcoT14I	CCWWGG	1 cut(s)	947
EcoT38I	GRGCYC	2 cut(s)	122, 929
ErhI	CCWWGG	1 cut(s)	947
Esp3I	CGTCTC	1 cut(s)	138
FaeI	CATG	9 cut(s)	39, 118, 937, 951, 1184, 1469, 1570, 1588, 1661
FalI	AAGNNNNNCTT	4 cut(s)	380, 412, 1919, 1951
FaqI	GGGAC	3 cut(s)	599, 1255, 1294
FatI	CATG	9 cut(s)	35, 114, 933, 947, 1180, 1465, 1566, 1584, 1657
FauI	CCCGC	1 cut(s)	1281
FauNDI	CATATG	1 cut(s)	165
FblI	GTMKAC	1 cut(s)	111
Fnu4HI	GCNGC	8 cut(s)	252, 624, 708, 966, 1317, 1416, 1527, 1674
FokI	GGATG	1 cut(s)	1533
FriOI	GRGCYC	2 cut(s)	122, 929
Fsp4HI	GCNGC	8 cut(s)	252, 624, 708, 966, 1317, 1416, 1527, 1674
GluI	GCNGC	8 cut(s)	252, 624, 708, 966, 1317, 1416, 1527, 1674
HaeIII	GGCC	5 cut(s)	254, 401, 448, 597, 1600
HapII	CCGG	5 cut(s)	189, 317, 519, 923, 1614
HgaI	GACGC	1 cut(s)	336
Hin1I	GRCGYC	1 cut(s)	347
Hin1II	CATG	9 cut(s)	39, 118, 937, 951, 1184, 1469, 1570, 1588, 1661
HincII	GTYRAC	3 cut(s)	790, 1258, 1663
HindII	GTYRAC	3 cut(s)	790, 1258, 1663
HpaI	GTTAAC	1 cut(s)	1258
HpaII	CCGG	5 cut(s)	189, 317, 519, 923, 1614
HphI	GGTGA	7 cut(s)	178, 229, 985, 1135, 1416, 1428, 1787
Hpy166II	GTNNAC	7 cut(s)	112, 605, 738, 790, 1258, 1508, 1663
Hpy8I	GTNNAC	7 cut(s)	112, 605, 738, 790, 1258, 1508, 1663
Hpy99I	CGWCG	2 cut(s)	133, 346
HpyCH4IV	ACGT	7 cut(s)	830, 1194, 1254, 1512, 1541, 1896, 2097
HpyCH4V	TGCA	8 cut(s)	71, 895, 965, 1160, 1517, 1628, 1791, 2066
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 620, 632, 716, 857, 866, 1523, 1649, 1778
HpyF3I	CTNAG	5 cut(s)	264, 492, 820, 928, 1106
HpySE526I	ACGT	7 cut(s)	830, 1194, 1254, 1512, 1541, 1896, 2097
Hsp92I	GRCGYC	1 cut(s)	347
Hsp92II	CATG	9 cut(s)	39, 118, 937, 951, 1184, 1469, 1570, 1588, 1661
Kpn2I	TCCGGA	1 cut(s)	316
KpnI	GGTACC	1 cut(s)	1905
KroI	GCCGGC	1 cut(s)	518
KroNI	GCCGGC	1 cut(s)	520
KspAI	GTTAAC	1 cut(s)	1258
Kzo9I	GATC	3 cut(s)	951, 1569, 1946
LmnI	GCTCC	5 cut(s)	554, 1324, 1649, 1865, 2034
Lsp1109I	GCAGC	6 cut(s)	719, 977, 1328, 1402, 1513, 1685
LweI	GCATC	4 cut(s)	169, 760, 810, 1637
MaeII	ACGT	7 cut(s)	830, 1194, 1254, 1512, 1541, 1896, 2097
MalI	GATC	3 cut(s)	953, 1571, 1948
MboI	GATC	3 cut(s)	951, 1569, 1946
MboII	GAAGA	4 cut(s)	167, 1142, 1332, 2052
MflI	RGATCY	2 cut(s)	951, 1946
MhlI	GDGCHC	2 cut(s)	122, 929
MlsI	TGGCCA	2 cut(s)	401, 448
MluCI	AATT	5 cut(s)	57, 1066, 1174, 1432, 1841
MluNI	TGGCCA	2 cut(s)	401, 448
MlyI	GAGTC	3 cut(s)	252, 275, 1232
Mox20I	TGGCCA	2 cut(s)	401, 448
MroI	TCCGGA	1 cut(s)	316
MroNI	GCCGGC	1 cut(s)	518
MscI	TGGCCA	2 cut(s)	401, 448
MseI	TTAA	7 cut(s)	324, 639, 1055, 1257, 1308, 1377, 1937
MslI	CAYNNNNRTG	2 cut(s)	152, 1185
Msp20I	TGGCCA	2 cut(s)	401, 448
MspA1I	CMGCKG	2 cut(s)	623, 902
MspCI	CTTAAG	1 cut(s)	1376
MspI	CCGG	5 cut(s)	189, 317, 519, 923, 1614
MspR9I	CCNGG	5 cut(s)	583, 924, 1313, 1398, 1686
Mva1269I	GAATGC	2 cut(s)	1200, 1612
MvaI	CCWGG	4 cut(s)	583, 1313, 1398, 1686
MvnI	CGCG	1 cut(s)	1347
MwoI	GCNNNNNNNGC	9 cut(s)	11, 620, 632, 716, 857, 866, 1523, 1649, 1778
NaeI	GCCGGC	1 cut(s)	520
NciI	CCSGG	1 cut(s)	924
NcoI	CCATGG	1 cut(s)	947
NdeI	CATATG	1 cut(s)	165
NdeII	GATC	3 cut(s)	951, 1569, 1946
NgoMIV	GCCGGC	1 cut(s)	518
NlaIII	CATG	9 cut(s)	39, 118, 937, 951, 1184, 1469, 1570, 1588, 1661
NlaIV	GGNNCC	4 cut(s)	580, 843, 1903, 2089
NmuCI	GTSAC	7 cut(s)	73, 184, 991, 1783, 1870, 2020, 2135
NspI	RCATGY	2 cut(s)	1469, 1661
PagI	TCATGA	2 cut(s)	933, 1584
PciI	ACATGT	2 cut(s)	1465, 1657
PcsI	WCGNNNNNNNCGW	2 cut(s)	429, 1518
PctI	GAATGC	2 cut(s)	1200, 1612
PdiI	GCCGGC	1 cut(s)	520
Pfl23II	CGTACG	1 cut(s)	831
PflFI	GACNNNGTC	2 cut(s)	346, 1241
PkrI	GCNGC	8 cut(s)	253, 625, 709, 967, 1318, 1417, 1528, 1675
PleI	GAGTC	3 cut(s)	252, 274, 1232
PpsI	GAGTC	3 cut(s)	252, 274, 1232
Ppu21I	YACGTR	1 cut(s)	831
PscI	ACATGT	2 cut(s)	1465, 1657
PshAI	GACNNNNGTC	1 cut(s)	1025
Psp6I	CCWGG	4 cut(s)	581, 1311, 1396, 1684
PspEI	GGTNACC	1 cut(s)	184
PspGI	CCWGG	4 cut(s)	581, 1311, 1396, 1684
PspLI	CGTACG	1 cut(s)	831
PspN4I	GGNNCC	4 cut(s)	580, 843, 1903, 2089
PspPI	GGNCC	6 cut(s)	253, 482, 841, 914, 1097, 1349
PstI	CTGCAG	1 cut(s)	73
PstNI	CAGNNNCTG	3 cut(s)	68, 626, 2024
PsuI	RGATCY	2 cut(s)	951, 1946
PsyI	GACNNNGTC	2 cut(s)	346, 1241
PvuII	CAGCTG	1 cut(s)	902
RsaI	GTAC	8 cut(s)	604, 739, 753, 833, 1005, 1475, 1532, 1903
RsaNI	GTAC	8 cut(s)	603, 738, 752, 832, 1004, 1474, 1531, 1902
RseI	CAYNNNNRTG	2 cut(s)	152, 1185
SaqAI	TTAA	7 cut(s)	324, 639, 1055, 1257, 1308, 1377, 1937
SatI	GCNGC	8 cut(s)	252, 624, 708, 966, 1317, 1416, 1527, 1674
Sau3AI	GATC	3 cut(s)	951, 1569, 1946
Sau96I	GGNCC	6 cut(s)	253, 482, 841, 914, 1097, 1349
ScaI	AGTACT	1 cut(s)	1475
SchI	GAGTC	3 cut(s)	252, 275, 1232
ScrFI	CCNGG	5 cut(s)	583, 924, 1313, 1398, 1686
SduI	GDGCHC	2 cut(s)	122, 929
SfaNI	GCATC	4 cut(s)	169, 760, 810, 1637
SfcI	CTRYAG	2 cut(s)	69, 721
SinI	GGWCC	5 cut(s)	482, 841, 914, 1097, 1349
SmiMI	CAYNNNNRTG	2 cut(s)	152, 1185
SmlI	CTYRAG	4 cut(s)	87, 697, 1376, 1443
SmoI	CTYRAG	4 cut(s)	87, 697, 1376, 1443
Sse9I	AATT	5 cut(s)	57, 1066, 1174, 1432, 1841
SsiI	CCGC	8 cut(s)	251, 516, 623, 826, 1274, 1347, 1364, 1622
StyD4I	CCNGG	5 cut(s)	581, 922, 1311, 1396, 1684
StyI	CCWWGG	1 cut(s)	947
TaiI	ACGT	7 cut(s)	833, 1197, 1257, 1515, 1544, 1899, 2100
TaqI	TCGA	5 cut(s)	136, 432, 765, 1962, 2043
TasI	AATT	5 cut(s)	57, 1066, 1174, 1432, 1841
TatI	WGTACW	3 cut(s)	751, 1003, 1473
TauI	GCSGC	2 cut(s)	254, 626
Tru1I	TTAA	7 cut(s)	324, 639, 1055, 1257, 1308, 1377, 1937
Tru9I	TTAA	7 cut(s)	324, 639, 1055, 1257, 1308, 1377, 1937
TscAI	CASTG	8 cut(s)	78, 268, 442, 748, 895, 1798, 2001, 2025
TseFI	GTSAC	7 cut(s)	73, 184, 991, 1783, 1870, 2020, 2135
TseI	GCWGC	6 cut(s)	707, 965, 1316, 1415, 1526, 1673
Tsp45I	GTSAC	7 cut(s)	73, 184, 991, 1783, 1870, 2020, 2135
TspDTI	ATGAA	7 cut(s)	418, 1185, 1535, 1619, 1713, 1855, 2003
TspGWI	ACGGA	4 cut(s)	117, 672, 854, 1959
TspRI	CASTG	8 cut(s)	78, 268, 442, 748, 895, 1798, 2001, 2025
Tth111I	GACNNNGTC	2 cut(s)	346, 1241
Vha464I	CTTAAG	1 cut(s)	1376
VpaK11BI	GGWCC	5 cut(s)	482, 841, 914, 1097, 1349
XapI	RAATTY	1 cut(s)	1432
XceI	RCATGY	2 cut(s)	1469, 1661
XmiI	GTMKAC	1 cut(s)	111
ZrmI	AGTACT	1 cut(s)	1475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.