Rroxscaffold_4G00326700

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000257
Physical Location & Seq
Forward (+)
58842022 .. 58844178
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326700.1

Sequence Viewer

Length: 2157 bp
ATGAGCTTATTGTGCAACGCCATTGATCATGAAGATAGAAAGGTTCATATAGTGTATCTGGGGTCACTTCCTGATGAGGAGGAGTACTCGCCAAGGTCTCGTCACCTCGATATACTACAAAGAGTTGTTGATGTCAGTTCTGCTGAAAATTGCTTGATAAGGAGCTACCAAAGGAGCTTCAATGGATTTGCTGCAAAGCTCAGTGATCAAGAAAAAGAAAAGATTGCTAACATGGAGGAGGTGGTCTCCGTCTTTCCTAGCAGAACCTTCCAACTTCAAACGACGAGGTCATGGGACTTCATGGGTCTCAAGCTGTCAAGTCCTCGAAAAGCCACTGCTGAGAGTAATGTTATTGTTGCTGTGATTGACACTGGAATTGACCCTGATTCGGAGAGCTTTAGAGATGAAGGTTTTGGTGCTGCTCCTGTGAAGTGGAAAGGTGTTTGTGAAGGTGGCGAAAATTTCAGTTGCAACAACAAGATAATTGGAGCTCGTTTTTACGAACCAGCAGAGTCTGCAAGGGACGAAGACGGCCATGGAACCCACACTGCCTCAACGGCAGTAGGCAATGAGGTAAGGGATGTAAGCTTTTACGGATTAGAACCAGGTACAGCAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGCGTATAAAGTCTGTGGGGCTACAGGGTGCTCTTCAGAGGCTATATTGGCTGCTTTTGACGATGCAATTGCTGATGGAGTTGACATTGTGTCAGTCTCACTTGGAAGTGCAAGTCCCCTTCCTTTTGATAATGATCCTATAGCAATTGGTTCTTATCATGCAATGAAGAGAGGGATATTGACATCAAACTCTGCAGGCAACAGTGGTCCTGGAGCTGGTACTGTAACAAGTGTAGCGCCATGGATGCTTGCAGTTGCAGCAAGTAGCAAAGATCGTCGGATCATTGACAAGGCCGTTCTTGGAAGTAGGACACTAGTAGGGGCTTCAGTGAACTCTTTCATATTGAAAGGTAGTTTTCCACTGATATATGGAAAAGATGCTTCAAGTAACTGCCCCGAATACTATGCTAGGACTTGTGCAGATGGTTGCCTAGACAGTAATTCAGTTCGGAACAAGATTGTGCTATGTGACCGGCCCGCTGGATTTTATGAGGCTGATCGAGCTGGTGCACAAGGCACAATTTTCTACAATCCCATACCTGATGATGCTTCTCTTGTCCCAATGCCTGCTGTGGGTTTGAGATCCGAAGACTATAATGTGGCCATATCTTACATGAACTCGACAAAAGATCCTCAAGCAAGCATATCAAAGAGTGAAAACGTAAAAGATCCCGCTGCACCTGAAGTTGTTTCTTTCTCTTCGCGTGGACCTAATCAAATTTTACCTGAAATTATTAAACCAGATGTAACTGGCCCGGGAGTGACCATTATAGCTGCATATCCGAGTGATGTTCCTGTCACAAGTGGCTCTAGAGACACAAGGCGGGTCAAGTACAATATACTATCCGGAACCTCCATGTCTTGCCCCCACGCTACTGGTGTAGCTGCATATGTAAAAGAGTTCCACCCTGATTGGTCTCCATCTGCCATTAAATCTGCTGTTATGACTACAGCTTGGCCTATGAATCATACAACCAACAGCTCATGCCCCGGTGAATTTGCATATGGATCCGGTCATATCAATCCTCTAAAAGCTATTGACCCAGGGCTTGTGTATGATGCTTCTGCAGAGGATTACATTAAGTTACTCTGTTTGGGCATGGACGAGGCGAGAGTCAGACTTATATCAGGAGATAACAGCACTTGCCCTACAGCTTCTGAACAAGGATCGGCAAAGGATCTCAACTATCCTTCAATATCAGCTATTGTTACACCAATGACACGTTTTACGGTGAAAATACGTAGAAGAGTTACAAATGTTGGCCTTGCAAATTCCACTTACAAGGCCGAAATCATGCCAAACTCTCAAGTTGACATCAAAGTGGAGCCTCAAGTTCTTTGCTTCAAGTCCGTAAACGAGGAGAAGACTTTTGATTTGACCATTGTTGGAAGAGGGTTGCCAGATGGATCACATATGTCTTCGTCCTTGGTTTGGTCCGACGAAACTCATAAGGTTAAAATTCCAGTTCTAGTGCAAAGCTTATCAGCAGCAGCATCATCACGGTTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

718

Amino Acids

76.84

Weight (kDa)

5.58

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 15 - 93 2.2e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 115 - 539 7.6e-45 Subtilase family
fn3_6 PF17766 608 - 706 4.7e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1348
AccIII TCCGGA 1 cut(s) 1490
AciI CCGC 3 cut(s) 1122, 1317, 1468
AcoI YGGCCR 2 cut(s) 532, 1245
AcsI RAATTY 5 cut(s) 460, 1362, 1640, 1915, 2103
AcuI CTGAAG 3 cut(s) 663, 954, 1347
AfaI GTAC 4 cut(s) 86, 610, 865, 1478
AfiI CCNNNNNNNGG 4 cut(s) 388, 860, 1217, 2076
AflIII ACRYGT 1 cut(s) 1865
AgsI TTSAA 6 cut(s) 181, 278, 991, 1029, 1839, 1990
AhdI GACNNNNNGTC 1 cut(s) 733
AhlI ACTAGT 1 cut(s) 958
AjnI CCWGG 3 cut(s) 604, 853, 1687
Alw21I GWGCWC 3 cut(s) 493, 677, 1156
Alw26I GTCTC 6 cut(s) 102, 250, 311, 745, 1452, 1566
Alw44I GTGCAC 1 cut(s) 1152
AlwNI CAGNNNCTG 2 cut(s) 515, 1802
Ama87I CYCGRG 1 cut(s) 1399
Aor13HI TCCGGA 1 cut(s) 1490
AoxI GGCC 8 cut(s) 532, 936, 1118, 1245, 1396, 1601, 1906, 1929
ApaLI GTGCAC 1 cut(s) 1152
ApoI RAATTY 5 cut(s) 460, 1362, 1640, 1915, 2103
ArsI GACNNNNNNTTYG 2 cut(s) 2002, 2034
Asp700I GAANNNNTTC 1 cut(s) 980
AspLEI GCGC 1 cut(s) 883
AspS9I GGNCC 5 cut(s) 851, 1119, 1352, 1397, 2079
AsuC2I CCSGG 3 cut(s) 1400, 1401, 1635
AsuHPI GGTGA 3 cut(s) 95, 1649, 1888
AvaI CYCGRG 1 cut(s) 1399
AvaII GGWCC 3 cut(s) 851, 1352, 2079
BaeGI GKGCMC 1 cut(s) 1156
BalI TGGCCA 1 cut(s) 1247
BamHI GGATCC 1 cut(s) 1652
BanII GRGCYC 1 cut(s) 493
BbsI GAAGAC 4 cut(s) 534, 1239, 2015, 2055
Bbv12I GWGCWC 3 cut(s) 493, 677, 1156
BbvCI CCTCAGC 1 cut(s) 631
BccI CCATC 4 cut(s) 713, 1061, 1573, 2042
BceAI ACGGC 3 cut(s) 547, 573, 923
BcgI CGANNNNNNTGC 4 cut(s) 695, 729, 1031, 1065
BciT130I CCWGG 3 cut(s) 606, 855, 1689
BclI TGATCA 2 cut(s) 25, 205
BcnI CCSGG 3 cut(s) 1400, 1401, 1635
BcoDI GTCTC 6 cut(s) 102, 250, 311, 745, 1452, 1566
BcuI ACTAGT 1 cut(s) 958
BfaI CTAG 6 cut(s) 258, 959, 1053, 1076, 1455, 2114
BfmI CTRYAG 6 cut(s) 666, 783, 837, 1593, 1710, 1794
BfoI RGCGCY 1 cut(s) 884
BglI GCCNNNNNGGC 1 cut(s) 557
BmcAI AGTACT 1 cut(s) 86
Bme1390I CCNGG 6 cut(s) 606, 855, 1400, 1401, 1635, 1689
Bme18I GGWCC 3 cut(s) 851, 1352, 2079
BmeRI GACNNNNNGTC 1 cut(s) 733
BmeT110I CYCGRG 1 cut(s) 1399
BmgT120I GGNCC 5 cut(s) 851, 1119, 1352, 1397, 2079
BmiI GGNNCC 5 cut(s) 541, 627, 1495, 1654, 1971
BmrFI CCNGG 6 cut(s) 606, 855, 1400, 1401, 1635, 1689
BmsI GCATC 6 cut(s) 697, 879, 1012, 1180, 1693, 2147
BpiI GAAGAC 4 cut(s) 534, 1239, 2015, 2055
BplI GAGNNNNNCTC 4 cut(s) 71, 103, 230, 262
BpmI CTGGAG 1 cut(s) 876
Bpu10I CCTNAGC 1 cut(s) 631
BpuEI CTTGAG 4 cut(s) 293, 1263, 1935, 1959
BpuMI CCSGG 3 cut(s) 1400, 1401, 1635
BsaAI YACGTR 1 cut(s) 1886
BsaBI GATNNNNATC 1 cut(s) 777
BsaI GGTCTC 4 cut(s) 102, 250, 311, 1566
BsaJI CCNNGG 8 cut(s) 92, 535, 884, 1399, 1633, 1687, 1688, 2070
BsaWI WCCGGW 2 cut(s) 1490, 1655
Bsc4I CCNNNNNNNGG 4 cut(s) 388, 860, 1217, 2076
Bse118I RCCGGY 1 cut(s) 1116
Bse1I ACTGG 4 cut(s) 376, 1399, 1525, 2108
Bse3DI GCAATG 2 cut(s) 574, 813
Bse8I GATNNNNATC 1 cut(s) 777
BseAI TCCGGA 1 cut(s) 1490
BseBI CCWGG 3 cut(s) 606, 855, 1689
BseDI CCNNGG 8 cut(s) 92, 535, 884, 1399, 1633, 1687, 1688, 2070
BseGI GGATG 2 cut(s) 586, 894
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 4 cut(s) 388, 860, 1217, 2076
BseMI GCAATG 2 cut(s) 574, 813
BseMII CTCAG 3 cut(s) 214, 330, 645
BseNI ACTGG 4 cut(s) 376, 1399, 1525, 2108
BseRI GAGGAG 5 cut(s) 92, 95, 251, 633, 2018
BseSI GKGCMC 1 cut(s) 1156
BsgI GTGCAG 2 cut(s) 1083, 1305
Bsh1236I CGCG 1 cut(s) 1348
BshFI GGCC 8 cut(s) 534, 938, 1120, 1247, 1398, 1603, 1908, 1931
BsiHKAI GWGCWC 3 cut(s) 493, 677, 1156
BsiHKCI CYCGRG 1 cut(s) 1399
BsiSI CCGG 5 cut(s) 1117, 1400, 1491, 1635, 1656
BslFI GGGAC 4 cut(s) 308, 536, 744, 1187
BslI CCNNNNNNNGG 4 cut(s) 388, 860, 1217, 2076
BsmAI GTCTC 6 cut(s) 102, 250, 311, 745, 1452, 1566
BsmFI GGGAC 4 cut(s) 308, 536, 744, 1187
BsnI GGCC 8 cut(s) 534, 938, 1120, 1247, 1398, 1603, 1908, 1931
Bso31I GGTCTC 4 cut(s) 102, 250, 311, 1566
BsoBI CYCGRG 1 cut(s) 1399
Bsp1286I GDGCHC 3 cut(s) 493, 677, 1156
Bsp13I TCCGGA 1 cut(s) 1490
Bsp19I CCATGG 2 cut(s) 535, 884
BspACI CCGC 3 cut(s) 1122, 1317, 1468
BspANI GGCC 8 cut(s) 534, 938, 1120, 1247, 1398, 1603, 1908, 1931
BspCNI CTCAG 3 cut(s) 213, 331, 644
BspEI TCCGGA 1 cut(s) 1490
BspFNI CGCG 1 cut(s) 1348
BspHI TCATGA 1 cut(s) 28
BspLI GGNNCC 5 cut(s) 541, 627, 1495, 1654, 1971
BspMAI CTGCAG 2 cut(s) 841, 1714
BspQI GCTCTTC 1 cut(s) 682
BspTNI GGTCTC 4 cut(s) 102, 250, 311, 1566
BsrDI GCAATG 2 cut(s) 574, 813
BsrFI RCCGGY 1 cut(s) 1116
BsrI ACTGG 4 cut(s) 376, 1399, 1525, 2108
BssAI RCCGGY 1 cut(s) 1116
BssECI CCNNGG 8 cut(s) 92, 535, 884, 1399, 1633, 1687, 1688, 2070
BssT1I CCWWGG 4 cut(s) 92, 535, 884, 2070
Bst2UI CCWGG 3 cut(s) 606, 855, 1689
Bst4CI ACNGT 5 cut(s) 848, 868, 1082, 1876, 2148
Bst6I CTCTTC 5 cut(s) 682, 806, 1348, 1885, 2029
BstAPI GCANNNNNTGC 1 cut(s) 515
BstBAI YACGTR 1 cut(s) 1886
BstC8I GCNNGC 5 cut(s) 841, 894, 1122, 1212, 1285
BstDEI CTNAG 3 cut(s) 200, 339, 631
BstDSI CCRYGG 2 cut(s) 535, 884
BstF5I GGATG 2 cut(s) 586, 894
BstFNI CGCG 1 cut(s) 1348
BstH2I RGCGCY 1 cut(s) 884
BstHHI GCGC 1 cut(s) 883
BstMAI GTCTC 6 cut(s) 102, 250, 311, 745, 1452, 1566
BstMWI GCNNNNNNNGC 8 cut(s) 12, 515, 557, 641, 692, 889, 902, 1145
BstNI CCWGG 3 cut(s) 606, 855, 1689
BstSCI CCNGG 6 cut(s) 604, 853, 1398, 1399, 1633, 1687
BstSFI CTRYAG 6 cut(s) 666, 783, 837, 1593, 1710, 1794
BstSLI GKGCMC 1 cut(s) 1156
BstSNI TACGTA 1 cut(s) 1886
BstUI CGCG 1 cut(s) 1348
BstV2I GAAGAC 4 cut(s) 534, 1239, 2015, 2055
BstX2I RGATCY 5 cut(s) 1226, 1273, 1312, 1652, 1822
BstXI CCANNNNNNTGG 1 cut(s) 1520
BstYI RGATCY 5 cut(s) 1226, 1273, 1312, 1652, 1822
BsuRI GGCC 8 cut(s) 534, 938, 1120, 1247, 1398, 1603, 1908, 1931
BtgI CCRYGG 2 cut(s) 535, 884
BtsCI GGATG 2 cut(s) 586, 894
BtsI GCAGTG 2 cut(s) 333, 546
BtsIMutI CAGTG 7 cut(s) 208, 333, 369, 546, 853, 978, 1004
Cac8I GCNNGC 5 cut(s) 841, 894, 1122, 1212, 1285
CaiI CAGNNNCTG 2 cut(s) 515, 1802
CciI TCATGA 1 cut(s) 28
CfoI GCGC 1 cut(s) 883
Cfr10I RCCGGY 1 cut(s) 1116
Cfr13I GGNCC 5 cut(s) 851, 1119, 1352, 1397, 2079
Cfr9I CCCGGG 1 cut(s) 1399
CsiI ACCWGGT 1 cut(s) 604
Csp6I GTAC 4 cut(s) 85, 609, 864, 1477
CspCI CAANNNNNGTGG 2 cut(s) 1538, 1573
CviQI GTAC 4 cut(s) 85, 609, 864, 1477
DdeI CTNAG 3 cut(s) 200, 339, 631
DriI GACNNNNNGTC 1 cut(s) 733
EaeI YGGCCR 2 cut(s) 532, 1245
Eam1104I CTCTTC 5 cut(s) 682, 806, 1348, 1885, 2029
Eam1105I GACNNNNNGTC 1 cut(s) 733
EarI CTCTTC 5 cut(s) 682, 806, 1348, 1885, 2029
Ecl136II GAGCTC 1 cut(s) 491
Eco105I TACGTA 1 cut(s) 1886
Eco130I CCWWGG 4 cut(s) 92, 535, 884, 2070
Eco24I GRGCYC 1 cut(s) 493
Eco31I GGTCTC 4 cut(s) 102, 250, 311, 1566
Eco47I GGWCC 3 cut(s) 851, 1352, 2079
Eco53kI GAGCTC 1 cut(s) 491
Eco57I CTGAAG 3 cut(s) 663, 954, 1347
Eco88I CYCGRG 1 cut(s) 1399
EcoICRI GAGCTC 1 cut(s) 491
EcoRII CCWGG 3 cut(s) 604, 853, 1687
EcoT14I CCWWGG 4 cut(s) 92, 535, 884, 2070
EcoT38I GRGCYC 1 cut(s) 493
ErhI CCWWGG 4 cut(s) 92, 535, 884, 2070
FaqI GGGAC 4 cut(s) 308, 536, 744, 1187
FauI CCCGC 3 cut(s) 1129, 1324, 1461
FauNDI CATATG 3 cut(s) 1534, 1648, 2058
FbaI TGATCA 2 cut(s) 25, 205
FokI GGATG 2 cut(s) 593, 901
FriOI GRGCYC 1 cut(s) 493
FspBI CTAG 6 cut(s) 258, 959, 1053, 1076, 1455, 2114
GlaI GCGC 1 cut(s) 882
GsuI CTGGAG 1 cut(s) 876
HaeII RGCGCY 1 cut(s) 884
HaeIII GGCC 8 cut(s) 534, 938, 1120, 1247, 1398, 1603, 1908, 1931
HapII CCGG 5 cut(s) 1117, 1400, 1491, 1635, 1656
HhaI GCGC 1 cut(s) 883
Hin6I GCGC 1 cut(s) 881
HinP1I GCGC 1 cut(s) 881
HincII GTYRAC 2 cut(s) 727, 1957
HindII GTYRAC 2 cut(s) 727, 1957
HindIII AAGCTT 2 cut(s) 586, 2122
HinfI GANTC 4 cut(s) 386, 512, 1609, 1758
HpaII CCGG 5 cut(s) 1117, 1400, 1491, 1635, 1656
HphI GGTGA 3 cut(s) 95, 1649, 1888
Hpy166II GTNNAC 6 cut(s) 727, 976, 1154, 1352, 1957, 1999
Hpy188I TCNGA 9 cut(s) 391, 682, 924, 1095, 1231, 1428, 1763, 1804, 2083
Hpy188III TCNNGA 6 cut(s) 29, 71, 209, 1455, 1491, 1773
Hpy8I GTNNAC 6 cut(s) 727, 976, 1154, 1352, 1957, 1999
Hpy99I CGWCG 3 cut(s) 286, 924, 2087
HpyAV CCTTC 5 cut(s) 277, 401, 443, 773, 1845
HpyCH4III ACNGT 5 cut(s) 848, 868, 1082, 1876, 2148
HpyCH4IV ACGT 3 cut(s) 1305, 1867, 1885
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 515, 557, 641, 692, 889, 902, 1145
HpyF3I CTNAG 3 cut(s) 200, 339, 631
HpySE526I ACGT 3 cut(s) 1305, 1867, 1885
HspAI GCGC 1 cut(s) 881
Kpn2I TCCGGA 1 cut(s) 1490
Ksp22I TGATCA 2 cut(s) 25, 205
LguI GCTCTTC 1 cut(s) 682
LmnI GCTCC 6 cut(s) 162, 174, 427, 488, 857, 1969
LweI GCATC 6 cut(s) 697, 879, 1012, 1180, 1693, 2147
MabI ACCWGGT 1 cut(s) 604
MaeI CTAG 6 cut(s) 258, 959, 1053, 1076, 1455, 2114
MaeII ACGT 3 cut(s) 1305, 1867, 1885
MfeI CAATTG 2 cut(s) 711, 789
MflI RGATCY 5 cut(s) 1226, 1273, 1312, 1652, 1822
MhlI GDGCHC 3 cut(s) 493, 677, 1156
MlsI TGGCCA 1 cut(s) 1247
MluNI TGGCCA 1 cut(s) 1247
MlyI GAGTC 2 cut(s) 521, 1767
MmeI TCCRAC 4 cut(s) 295, 902, 2011, 2106
Mox20I TGGCCA 1 cut(s) 1247
MroI TCCGGA 1 cut(s) 1490
MroXI GAANNNNTTC 1 cut(s) 980
MscI TGGCCA 1 cut(s) 1247
MseI TTAA 5 cut(s) 1380, 1575, 1725, 2100, 2155
MslI CAYNNNNRTG 1 cut(s) 1964
Msp20I TGGCCA 1 cut(s) 1247
MspA1I CMGCKG 2 cut(s) 1124, 1319
MspI CCGG 5 cut(s) 1117, 1400, 1491, 1635, 1656
MspR9I CCNGG 6 cut(s) 606, 855, 1400, 1401, 1635, 1689
MunI CAATTG 2 cut(s) 711, 789
MvaI CCWGG 3 cut(s) 606, 855, 1689
MvnI CGCG 1 cut(s) 1348
MwoI GCNNNNNNNGC 8 cut(s) 12, 515, 557, 641, 692, 889, 902, 1145
NciI CCSGG 3 cut(s) 1400, 1401, 1635
NcoI CCATGG 2 cut(s) 535, 884
NdeI CATATG 3 cut(s) 1534, 1648, 2058
NlaIV GGNNCC 5 cut(s) 541, 627, 1495, 1654, 1971
NmuCI GTSAC 5 cut(s) 63, 101, 1112, 1405, 1441
PagI TCATGA 1 cut(s) 28
PasI CCCWGGG 1 cut(s) 1688
PciSI GCTCTTC 1 cut(s) 682
PdmI GAANNNNTTC 1 cut(s) 980
PfeI GAWTC 2 cut(s) 386, 1609
PflFI GACNNNGTC 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 853
PleI GAGTC 2 cut(s) 520, 1766
PpsI GAGTC 2 cut(s) 520, 1766
Ppu21I YACGTR 1 cut(s) 1886
Psp124BI GAGCTC 1 cut(s) 493
Psp6I CCWGG 3 cut(s) 604, 853, 1687
PspGI CCWGG 3 cut(s) 604, 853, 1687
PspN4I GGNNCC 5 cut(s) 541, 627, 1495, 1654, 1971
PspPI GGNCC 5 cut(s) 851, 1119, 1352, 1397, 2079
PstI CTGCAG 2 cut(s) 841, 1714
PstNI CAGNNNCTG 2 cut(s) 515, 1802
PsuI RGATCY 5 cut(s) 1226, 1273, 1312, 1652, 1822
PsyI GACNNNGTC 1 cut(s) 286
RsaI GTAC 4 cut(s) 86, 610, 865, 1478
RsaNI GTAC 4 cut(s) 85, 609, 864, 1477
RseI CAYNNNNRTG 1 cut(s) 1964
SacI GAGCTC 1 cut(s) 493
SapI GCTCTTC 1 cut(s) 682
SaqAI TTAA 5 cut(s) 1380, 1575, 1725, 2100, 2155
Sau96I GGNCC 5 cut(s) 851, 1119, 1352, 1397, 2079
ScaI AGTACT 1 cut(s) 86
SchI GAGTC 2 cut(s) 521, 1767
ScrFI CCNGG 6 cut(s) 606, 855, 1400, 1401, 1635, 1689
SduI GDGCHC 3 cut(s) 493, 677, 1156
SexAI ACCWGGT 1 cut(s) 604
SfaNI GCATC 6 cut(s) 697, 879, 1012, 1180, 1693, 2147
SfcI CTRYAG 6 cut(s) 666, 783, 837, 1593, 1710, 1794
SinI GGWCC 3 cut(s) 851, 1352, 2079
SmaI CCCGGG 1 cut(s) 1401
SmiMI CAYNNNNRTG 1 cut(s) 1964
SmlI CTYRAG 4 cut(s) 308, 1278, 1950, 1974
SmoI CTYRAG 4 cut(s) 308, 1278, 1950, 1974
SnaBI TACGTA 1 cut(s) 1886
SpeI ACTAGT 1 cut(s) 958
SsiI CCGC 3 cut(s) 1122, 1317, 1468
SspMI CTAG 6 cut(s) 258, 959, 1053, 1076, 1455, 2114
SstI GAGCTC 1 cut(s) 493
StyD4I CCNGG 6 cut(s) 604, 853, 1398, 1399, 1633, 1687
StyI CCWWGG 4 cut(s) 92, 535, 884, 2070
TaaI ACNGT 5 cut(s) 848, 868, 1082, 1876, 2148
TaiI ACGT 3 cut(s) 1308, 1870, 1888
TaqI TCGA 4 cut(s) 108, 325, 1144, 1265
TatI WGTACW 2 cut(s) 84, 1476
TfiI GAWTC 2 cut(s) 386, 1609
Tru1I TTAA 5 cut(s) 1380, 1575, 1725, 2100, 2155
Tru9I TTAA 5 cut(s) 1380, 1575, 1725, 2100, 2155
TscAI CASTG 7 cut(s) 208, 340, 376, 553, 853, 978, 1011
TseFI GTSAC 5 cut(s) 63, 101, 1112, 1405, 1441
Tsp45I GTSAC 5 cut(s) 63, 101, 1112, 1405, 1441
TspDTI ATGAA 9 cut(s) 35, 45, 289, 420, 824, 973, 1274, 1622, 2141
TspGWI ACGGA 3 cut(s) 238, 609, 1984
TspMI CCCGGG 1 cut(s) 1399
TspRI CASTG 7 cut(s) 208, 340, 376, 553, 853, 978, 1011
Tth111I GACNNNGTC 1 cut(s) 286
VneI GTGCAC 1 cut(s) 1152
VpaK11BI GGWCC 3 cut(s) 851, 1352, 2079
XapI RAATTY 5 cut(s) 460, 1362, 1640, 1915, 2103
XbaI TCTAGA 1 cut(s) 1454
XmaI CCCGGG 1 cut(s) 1399
XmnI GAANNNNTTC 1 cut(s) 980
XspI CTAG 6 cut(s) 258, 959, 1053, 1076, 1455, 2114
ZrmI AGTACT 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.