pycom02g24500

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000257
Physical Location & Seq
Forward (+)
22652481 .. 22654658
2178 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24500.1

Sequence Viewer

Length: 2178 bp
ATGGCAAAGCACGGAGCTCTCCTGTTTTCTTATGCTTTCTCCATTATTCTCTTGCTCACTTTGAGTTTACCGTCTGAAGCCGCTGATCAGGAAGAAAGAAAGACGTATATTATCTACTTGGGATCACTTCCTAAGGATGAGCTGTTCTCGCCATTGTCTCACCACATTGGTATTCTCGAAGGAGTTACCGAAAGTAGCTCTGCTTCGAATCTCTTGGTCAGAAGCTACAAAAAAAGCTTCAACGGATTCGCTGCAAAGCTCACTGACCGAGAAGGAGAAAAGCTTGCTAAGATGAAGGAAGTTGTCTCCGTGTTTCCGAGCAAAACTTACAAACTCCAAACAACAAGATCCTGGGAATTCTTGGGCTTCAATGAGAAGCACAAACGAAATCCTACTGTCGAGAGCAATGTTATTATTGGTGTCATCGATTCTGGGATATGGCCTGAATCTAAAAGTTTTAGCGATGAAGGTTTTGGTCCAGCTCCAAAGAAGTGGAAAGGAGTTTGCAAAGGAGGCAAAAATTTCACTTGCAACAACAAGATCATTGGAGCTCGGTTTTACACTGATTCCTCAGCTAGAGATGAGAATGGTCATGGAACTCATACTGCCTCAACTGCAGCAGGAAATCCGGTAAAGGGTGTGAGCTTTTATGGACTGGGGCAGGGAACTGCAAGAGGAGGCGTTCCCTCTGCGAGGATTGCTGCATATAAAGTTTGCAATTCTAAAGGTTGCTCTTCCGAAGGGCTCTTGGGTGCTTTTGATGACGCTATTCATGATGGAGTTGACATCATTACAATTTCAATTGGAGCCGAAACTGCAGCCCCTTTGGATCAGGATCCTATAGCAATCGGTGCTCTTCATGCAATTGAGAAAGGGATACTAACCTTGCAATCCGCAGGAAATAATGGCCCTGACGGAGGTTCTGTATCAAGTGTAGCACCATGGATTCTTGCAGTTGCAGCAAGTAGCACAGATCGTCGCATTATTGACAAGGTTATTCTTGGAAACAGGACGCTTGTCGGTAGTTCAGTGAATGCTTTCAAATTAAATGGAACAAGTTTTCCGTTGATAGAAGGCAAAGATGCAGGTCAATGCTTGGATTGTGAATTAGGTTGCATGGACCCTAATTTAGCTAAGGGAAAGATTGTGGTATGTTCTGATGCTGGGGGCAGTATTGAAGCTCATCGAGTCGGCGCAATAGGTGCGCTAACTAGCACTACCAAACCCGATGTTTCTTTCATTGTCCCACTACCTGCAGCAAGTTTTGGCAGTAAAGATTTTGGCGCCGTCGAGTCATATCTCAGCTCCACAGCCAACCCTCGAGCAAAGATACTAAAAAGTGAAGTCATAAAAGATCTTGCTGCACCTACTATCGCTTCCTTCTCTTCACGGGGGCCTAATTCTATTTTACCTGAAATTATCAAGCCGGACATAACTGCCCCGGGGGTTGATATTTTGGCTGCATATTCACCTGTTGCTCCTCTCACAGAGAGCTCTGAAGACCAGAGGCATGTAAAATACAGTATCTTATCTGGAACCTCCATGTCTTGCCCGCATGCTGCTGGTGTCGCTGCGTATATCAAGACATTCCACCCCAATTGGTCTCCATCTGCTATCAAATCATCTATTCTTACTACAGCTGGGCCTGTGAATGGCACCAACAATTCCACCTCACCGGGCGAATTTTCGTATGGATCTGGGCATATCAATCCTATAAAGGCTGTAAATCCGGGGCTTGTGTACGAAACGTCTAAGAATGATTACATTAAGTTGCTATGCTCTATCTTGGATGAGTCCAAAGTTCGACTCATTTCTGGAGACAAAGGCCCTTGCTCTGCAAGTACTGGCAATAAATCGGCCAAGGATCTCAACTACCCTTCGCTGGCTGGTAATGTTTCTCCGGAAACTTCATTTTCGATTAAGTTTCCAAGAACAGTAAAAAATGTTGGCGTTGCAAACTCCACATACAAGGCAAAGATCATCTCAAAGCTGGGAGTTAACATCAAGGTGGCGCCTCAAGTTCTTTCCTTCAAGTCTTTGAATGAGAAAAAGACCTTTAATGTGACAGTTTCTGGAAGAGGTATGCCATATGGTTCGTATGTATCTGCGTCATTGGTGTGGACGGATGGCACTCACAGCGTTCGAAGTCCAATTCTTGTAACCAGCTTCTCGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

726

Amino Acids

76.24

Weight (kDa)

8.94

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 113 1.6e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 555 5.8e-46 Subtilase family
fn3_6 PF17766 622 - 720 4.6e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1076, 1261
AccB1I GGYRCC 3 cut(s) 1283, 1655, 2011
AccIII TCCGGA 1 cut(s) 1900
AciI CCGC 3 cut(s) 81, 894, 1553
AclWI GGATC 7 cut(s) 130, 342, 830, 837, 843, 1702, 1872
AcoI YGGCCR 1 cut(s) 1857
AcsI RAATTY 3 cut(s) 356, 520, 1682
AcuI CTGAAG 2 cut(s) 96, 1518
AcyI GRCGYC 2 cut(s) 1284, 2012
AfaI GTAC 2 cut(s) 1742, 1843
AfiI CCNNNNNNNGG 5 cut(s) 635, 693, 917, 1652, 1882
AgsI TTSAA 7 cut(s) 241, 370, 801, 1042, 1178, 2032, 2041
AjnI CCWGG 1 cut(s) 350
AloI GAACNNNNNNTCC 4 cut(s) 128, 160, 1045, 1077
Alw21I GWGCWC 4 cut(s) 19, 553, 856, 1496
Alw26I GTCTC 4 cut(s) 162, 310, 1608, 1812
AlwI GGATC 7 cut(s) 130, 342, 830, 837, 843, 1702, 1872
AlwNI CAGNNNCTG 1 cut(s) 2072
Ama87I CYCGRG 2 cut(s) 1320, 1441
Aor13HI TCCGGA 1 cut(s) 1900
AoxI GGCC 6 cut(s) 440, 907, 1394, 1643, 1825, 1857
ApoI RAATTY 3 cut(s) 356, 520, 1682
Asp700I GAANNNNTTC 1 cut(s) 1037
AspLEI GCGC 4 cut(s) 1196, 1207, 1286, 2014
AspS9I GGNCC 6 cut(s) 476, 908, 1120, 1394, 1643, 1826
AsuC2I CCSGG 4 cut(s) 1442, 1443, 1677, 1731
AsuHPI GGTGA 3 cut(s) 152, 1461, 1665
AsuII TTCGAA 2 cut(s) 206, 2143
AvaI CYCGRG 2 cut(s) 1320, 1441
AvaII GGWCC 2 cut(s) 476, 1120
AxyI CCTNAGG 1 cut(s) 132
BamHI GGATCC 1 cut(s) 835
BanI GGYRCC 3 cut(s) 1283, 1655, 2011
BanII GRGCYC 4 cut(s) 19, 553, 747, 1496
BarI GAAGNNNNNNTAC 2 cut(s) 187, 219
BbsI GAAGAC 1 cut(s) 1506
Bbv12I GWGCWC 4 cut(s) 19, 553, 856, 1496
BbvCI CCTCAGC 1 cut(s) 571
BccI CCATC 3 cut(s) 770, 1615, 2120
BceAI ACGGC 1 cut(s) 1271
BciT130I CCWGG 1 cut(s) 352
BciVI GTATCC 1 cut(s) 870
BclI TGATCA 1 cut(s) 85
BcnI CCSGG 4 cut(s) 1442, 1443, 1677, 1731
BcoDI GTCTC 4 cut(s) 162, 310, 1608, 1812
BfaI CTAG 2 cut(s) 576, 1212
BfmI CTRYAG 5 cut(s) 615, 816, 840, 1254, 1635
BfoI RGCGCY 2 cut(s) 1287, 2015
BfuAI ACCTGC 2 cut(s) 1076, 1261
BfuI GTATCC 1 cut(s) 870
BglII AGATCT 1 cut(s) 1354
BmcAI AGTACT 1 cut(s) 1843
Bme1390I CCNGG 5 cut(s) 352, 1442, 1443, 1677, 1731
Bme18I GGWCC 2 cut(s) 476, 1120
BmeT110I CYCGRG 2 cut(s) 1320, 1441
BmgT120I GGNCC 6 cut(s) 476, 908, 1120, 1394, 1643, 1826
BmiI GGNNCC 8 cut(s) 808, 837, 1122, 1285, 1395, 1537, 1657, 2013
BmrFI CCNGG 5 cut(s) 352, 1442, 1443, 1677, 1731
BmrI ACTGGG 1 cut(s) 665
BmsI GCATC 2 cut(s) 1072, 1150
BmuI ACTGGG 1 cut(s) 665
BoxI GACNNNNGTC 1 cut(s) 1016
BpiI GAAGAC 1 cut(s) 1506
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 1 cut(s) 1836
Bpu10I CCTNAGC 2 cut(s) 571, 1134
Bpu14I TTCGAA 2 cut(s) 206, 2143
BpuEI CTTGAG 1 cut(s) 2001
BpuMI CCSGG 4 cut(s) 1442, 1443, 1677, 1731
Bsa29I ATCGAT 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 834
BsaHI GRCGYC 2 cut(s) 1284, 2012
BsaI GGTCTC 1 cut(s) 1608
BsaJI CCNNGG 7 cut(s) 351, 941, 1440, 1441, 1442, 1730, 1860
BsaWI WCCGGW 2 cut(s) 628, 1900
BsaXI ACNNNNNCTCC 2 cut(s) 909, 939
Bsc4I CCNNNNNNNGG 5 cut(s) 635, 693, 917, 1652, 1882
Bse1I ACTGG 2 cut(s) 660, 1849
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 1 cut(s) 412
Bse8I GATNNNNATC 1 cut(s) 834
BseAI TCCGGA 1 cut(s) 1900
BseBI CCWGG 1 cut(s) 352
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 7 cut(s) 351, 941, 1440, 1441, 1442, 1730, 1860
BseGI GGATG 3 cut(s) 142, 1795, 2131
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 5 cut(s) 635, 693, 917, 1652, 1882
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 2 cut(s) 585, 1315
BseNI ACTGG 2 cut(s) 660, 1849
BseRI GAGGAG 2 cut(s) 690, 1470
BseYI CCCAGC 3 cut(s) 1163, 1640, 1990
BsgI GTGCAG 1 cut(s) 1347
BshFI GGCC 6 cut(s) 442, 909, 1396, 1645, 1827, 1859
BshNI GGYRCC 3 cut(s) 1283, 1655, 2011
BshVI ATCGAT 1 cut(s) 426
BsiHKAI GWGCWC 4 cut(s) 19, 553, 856, 1496
BsiHKCI CYCGRG 2 cut(s) 1320, 1441
BsiSI CCGG 6 cut(s) 629, 1427, 1442, 1676, 1730, 1901
BslFI GGGAC 1 cut(s) 1229
BslI CCNNNNNNNGG 5 cut(s) 635, 693, 917, 1652, 1882
BsmAI GTCTC 4 cut(s) 162, 310, 1608, 1812
BsmFI GGGAC 1 cut(s) 1229
BsmI GAATGC 1 cut(s) 1039
BsnI GGCC 6 cut(s) 442, 909, 1396, 1645, 1827, 1859
Bso31I GGTCTC 1 cut(s) 1608
BsoBI CYCGRG 2 cut(s) 1320, 1441
Bsp119I TTCGAA 2 cut(s) 206, 2143
Bsp1286I GDGCHC 5 cut(s) 19, 553, 747, 856, 1496
Bsp13I TCCGGA 1 cut(s) 1900
Bsp19I CCATGG 1 cut(s) 941
BspACI CCGC 3 cut(s) 81, 894, 1553
BspANI GGCC 6 cut(s) 442, 909, 1396, 1645, 1827, 1859
BspCNI CTCAG 2 cut(s) 584, 1314
BspDI ATCGAT 1 cut(s) 426
BspEI TCCGGA 1 cut(s) 1900
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 8 cut(s) 808, 837, 1122, 1285, 1395, 1537, 1657, 2013
BspMAI CTGCAG 3 cut(s) 619, 820, 1258
BspMI ACCTGC 2 cut(s) 1076, 1261
BspPI GGATC 7 cut(s) 130, 342, 830, 837, 843, 1702, 1872
BspQI GCTCTTC 2 cut(s) 739, 861
BspT104I TTCGAA 2 cut(s) 206, 2143
BspT107I GGYRCC 3 cut(s) 1283, 1655, 2011
BspTNI GGTCTC 1 cut(s) 1608
BsrDI GCAATG 1 cut(s) 412
BsrI ACTGG 2 cut(s) 660, 1849
BssECI CCNNGG 7 cut(s) 351, 941, 1440, 1441, 1442, 1730, 1860
BssNI GRCGYC 2 cut(s) 1284, 2012
BssT1I CCWWGG 2 cut(s) 941, 1860
Bst2UI CCWGG 1 cut(s) 352
Bst4CI ACNGT 5 cut(s) 72, 397, 1523, 1936, 2068
Bst6I CTCTTC 4 cut(s) 739, 861, 1390, 2071
BstACI GRCGYC 2 cut(s) 1284, 2012
BstAPI GCANNNNNTGC 2 cut(s) 851, 1202
BstBI TTCGAA 2 cut(s) 206, 2143
BstC8I GCNNGC 4 cut(s) 285, 1553, 1557, 1884
BstDEI CTNAG 6 cut(s) 132, 288, 571, 1134, 1301, 1752
BstDSI CCRYGG 1 cut(s) 941
BstENI CCTNNNNNAGG 2 cut(s) 691, 915
BstF5I GGATG 3 cut(s) 142, 1795, 2131
BstH2I RGCGCY 2 cut(s) 1287, 2015
BstHHI GCGC 4 cut(s) 1196, 1207, 1286, 2014
BstMAI GTCTC 4 cut(s) 162, 310, 1608, 1812
BstNI CCWGG 1 cut(s) 352
BstNSI RCATGY 2 cut(s) 1514, 1559
BstPAI GACNNNNGTC 1 cut(s) 1016
BstSCI CCNGG 5 cut(s) 350, 1440, 1441, 1675, 1729
BstSFI CTRYAG 5 cut(s) 615, 816, 840, 1254, 1635
BstV2I GAAGAC 1 cut(s) 1506
BstX2I RGATCY 5 cut(s) 347, 835, 1354, 1694, 1864
BstXI CCANNNNNNTGG 1 cut(s) 492
BstYI RGATCY 5 cut(s) 347, 835, 1354, 1694, 1864
Bsu15I ATCGAT 1 cut(s) 426
Bsu36I CCTNAGG 1 cut(s) 132
BsuI GTATCC 1 cut(s) 870
BsuRI GGCC 6 cut(s) 442, 909, 1396, 1645, 1827, 1859
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 941
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 3 cut(s) 142, 1795, 2131
BtsIMutI CAGTG 3 cut(s) 261, 561, 1035
BveI ACCTGC 2 cut(s) 1076, 1261
Cac8I GCNNGC 4 cut(s) 285, 1553, 1557, 1884
CaiI CAGNNNCTG 1 cut(s) 2072
CciI TCATGA 1 cut(s) 772
CfoI GCGC 4 cut(s) 1196, 1207, 1286, 2014
Cfr13I GGNCC 6 cut(s) 476, 908, 1120, 1394, 1643, 1826
Cfr9I CCCGGG 1 cut(s) 1441
ClaI ATCGAT 1 cut(s) 426
CseI GACGC 3 cut(s) 773, 1021, 2097
Csp6I GTAC 2 cut(s) 1741, 1842
CspCI CAANNNNNGTGG 2 cut(s) 1580, 1615
CviAII CATG 8 cut(s) 593, 773, 860, 942, 1117, 1511, 1543, 1556
CviQI GTAC 2 cut(s) 1741, 1842
DdeI CTNAG 6 cut(s) 132, 288, 571, 1134, 1301, 1752
DinI GGCGCC 2 cut(s) 1285, 2013
EaeI YGGCCR 1 cut(s) 1857
Eam1104I CTCTTC 4 cut(s) 739, 861, 1390, 2071
EarI CTCTTC 4 cut(s) 739, 861, 1390, 2071
Ecl136II GAGCTC 3 cut(s) 17, 551, 1494
Eco130I CCWWGG 2 cut(s) 941, 1860
Eco24I GRGCYC 4 cut(s) 19, 553, 747, 1496
Eco31I GGTCTC 1 cut(s) 1608
Eco47I GGWCC 2 cut(s) 476, 1120
Eco53kI GAGCTC 3 cut(s) 17, 551, 1494
Eco57I CTGAAG 2 cut(s) 96, 1518
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 2 cut(s) 1320, 1441
EcoICRI GAGCTC 3 cut(s) 17, 551, 1494
EcoNI CCTNNNNNAGG 2 cut(s) 691, 915
EcoO109I RGGNCCY 2 cut(s) 1394, 1826
EcoRI GAATTC 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 350
EcoT14I CCWWGG 2 cut(s) 941, 1860
EcoT38I GRGCYC 4 cut(s) 19, 553, 747, 1496
EgeI GGCGCC 2 cut(s) 1285, 2013
EheI GGCGCC 2 cut(s) 1285, 2013
ErhI CCWWGG 2 cut(s) 941, 1860
FaeI CATG 8 cut(s) 596, 776, 863, 945, 1120, 1514, 1546, 1559
FaqI GGGAC 1 cut(s) 1229
FatI CATG 8 cut(s) 592, 772, 859, 941, 1116, 1510, 1542, 1555
FauI CCCGC 1 cut(s) 1560
FauNDI CATATG 1 cut(s) 2089
FbaI TGATCA 1 cut(s) 85
FokI GGATG 3 cut(s) 149, 1802, 2138
FriOI GRGCYC 4 cut(s) 19, 553, 747, 1496
FspBI CTAG 2 cut(s) 576, 1212
GlaI GCGC 4 cut(s) 1195, 1206, 1285, 2013
GsaI CCCAGC 3 cut(s) 1167, 1644, 1994
GsuI CTGGAG 1 cut(s) 1836
HaeII RGCGCY 2 cut(s) 1287, 2015
HaeIII GGCC 6 cut(s) 442, 909, 1396, 1645, 1827, 1859
HapII CCGG 6 cut(s) 629, 1427, 1442, 1676, 1730, 1901
HgaI GACGC 3 cut(s) 773, 1021, 2097
HhaI GCGC 4 cut(s) 1196, 1207, 1286, 2014
Hin1I GRCGYC 2 cut(s) 1284, 2012
Hin1II CATG 8 cut(s) 596, 776, 863, 945, 1120, 1514, 1546, 1559
Hin6I GCGC 4 cut(s) 1194, 1205, 1284, 2012
HinP1I GCGC 4 cut(s) 1194, 1205, 1284, 2012
HincII GTYRAC 2 cut(s) 784, 1999
HindII GTYRAC 2 cut(s) 784, 1999
HindIII AAGCTT 2 cut(s) 235, 281
HpaI GTTAAC 1 cut(s) 1999
HpaII CCGG 6 cut(s) 629, 1427, 1442, 1676, 1730, 1901
HphI GGTGA 3 cut(s) 152, 1461, 1665
Hpy166II GTNNAC 5 cut(s) 68, 784, 1741, 1999, 2121
Hpy188I TCNGA 6 cut(s) 76, 221, 318, 739, 1159, 1498
Hpy8I GTNNAC 5 cut(s) 68, 784, 1741, 1999, 2121
Hpy99I CGWCG 2 cut(s) 981, 1292
HpyAV CCTTC 9 cut(s) 173, 266, 289, 461, 734, 1067, 1390, 1887, 2038
HpyCH4III ACNGT 5 cut(s) 72, 397, 1523, 1936, 2068
HpyCH4IV ACGT 2 cut(s) 104, 1748
HpyF3I CTNAG 6 cut(s) 132, 288, 571, 1134, 1301, 1752
HpySE526I ACGT 2 cut(s) 104, 1748
Hsp92I GRCGYC 2 cut(s) 1284, 2012
Hsp92II CATG 8 cut(s) 596, 776, 863, 945, 1120, 1514, 1546, 1559
HspAI GCGC 4 cut(s) 1194, 1205, 1284, 2012
KasI GGCGCC 2 cut(s) 1283, 2011
Kpn2I TCCGGA 1 cut(s) 1900
Ksp22I TGATCA 1 cut(s) 85
KspAI GTTAAC 1 cut(s) 1999
LguI GCTCTTC 2 cut(s) 739, 861
LmnI GCTCC 6 cut(s) 14, 487, 548, 806, 1310, 1483
LweI GCATC 2 cut(s) 1072, 1150
MaeI CTAG 2 cut(s) 576, 1212
MaeII ACGT 2 cut(s) 104, 1748
MaeIII GTNAC 3 cut(s) 184, 2062, 2158
MboII GAAGA 6 cut(s) 104, 726, 848, 1377, 1511, 2088
MfeI CAATTG 3 cut(s) 801, 864, 1597
MflI RGATCY 5 cut(s) 347, 835, 1354, 1694, 1864
MhlI GDGCHC 5 cut(s) 19, 553, 747, 856, 1496
Mly113I GGCGCC 2 cut(s) 1284, 2012
MlyI GAGTC 4 cut(s) 1197, 1301, 1800, 1802
MroI TCCGGA 1 cut(s) 1900
MroXI GAANNNNTTC 1 cut(s) 1037
MseI TTAA 5 cut(s) 1046, 1767, 1920, 1998, 2058
MslI CAYNNNNRTG 2 cut(s) 2006, 2116
MspA1I CMGCKG 2 cut(s) 83, 1640
MspI CCGG 6 cut(s) 629, 1427, 1442, 1676, 1730, 1901
MspR9I CCNGG 5 cut(s) 352, 1442, 1443, 1677, 1731
MunI CAATTG 3 cut(s) 801, 864, 1597
Mva1269I GAATGC 1 cut(s) 1039
MvaI CCWGG 1 cut(s) 352
NarI GGCGCC 2 cut(s) 1284, 2012
NciI CCSGG 4 cut(s) 1442, 1443, 1677, 1731
NcoI CCATGG 1 cut(s) 941
NdeI CATATG 1 cut(s) 2089
NlaIII CATG 8 cut(s) 596, 776, 863, 945, 1120, 1514, 1546, 1559
NlaIV GGNNCC 8 cut(s) 808, 837, 1122, 1285, 1395, 1537, 1657, 2013
NmeAIII GCCGAG 1 cut(s) 2150
NmuCI GTSAC 1 cut(s) 2062
NspI RCATGY 2 cut(s) 1514, 1559
NspV TTCGAA 2 cut(s) 206, 2143
PaeI GCATGC 1 cut(s) 1559
PaeR7I CTCGAG 1 cut(s) 1320
PagI TCATGA 1 cut(s) 772
PciSI GCTCTTC 2 cut(s) 739, 861
PctI GAATGC 1 cut(s) 1039
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 6 cut(s) 208, 246, 428, 446, 566, 946
PleI GAGTC 4 cut(s) 1196, 1300, 1800, 1801
PluTI GGCGCC 2 cut(s) 1287, 2015
PpsI GAGTC 4 cut(s) 1196, 1300, 1800, 1801
PshAI GACNNNNGTC 1 cut(s) 1016
Psp124BI GAGCTC 3 cut(s) 19, 553, 1496
Psp6I CCWGG 1 cut(s) 350
PspFI CCCAGC 3 cut(s) 1163, 1640, 1990
PspGI CCWGG 1 cut(s) 350
PspN4I GGNNCC 8 cut(s) 808, 837, 1122, 1285, 1395, 1537, 1657, 2013
PspPI GGNCC 6 cut(s) 476, 908, 1120, 1394, 1643, 1826
PspXI VCTCGAGB 1 cut(s) 1320
PstI CTGCAG 3 cut(s) 619, 820, 1258
PstNI CAGNNNCTG 1 cut(s) 2072
PsuI RGATCY 5 cut(s) 347, 835, 1354, 1694, 1864
PvuII CAGCTG 1 cut(s) 1640
RsaI GTAC 2 cut(s) 1742, 1843
RsaNI GTAC 2 cut(s) 1741, 1842
RseI CAYNNNNRTG 2 cut(s) 2006, 2116
SacI GAGCTC 3 cut(s) 19, 553, 1496
SapI GCTCTTC 2 cut(s) 739, 861
SaqAI TTAA 5 cut(s) 1046, 1767, 1920, 1998, 2058
Sau96I GGNCC 6 cut(s) 476, 908, 1120, 1394, 1643, 1826
ScaI AGTACT 1 cut(s) 1843
SchI GAGTC 4 cut(s) 1197, 1301, 1800, 1802
ScrFI CCNGG 5 cut(s) 352, 1442, 1443, 1677, 1731
SduI GDGCHC 5 cut(s) 19, 553, 747, 856, 1496
SfaNI GCATC 2 cut(s) 1072, 1150
SfcI CTRYAG 5 cut(s) 615, 816, 840, 1254, 1635
SfoI GGCGCC 2 cut(s) 1285, 2013
Sfr274I CTCGAG 1 cut(s) 1320
SfuI TTCGAA 2 cut(s) 206, 2143
SinI GGWCC 2 cut(s) 476, 1120
SlaI CTCGAG 1 cut(s) 1320
SmaI CCCGGG 1 cut(s) 1443
SmiMI CAYNNNNRTG 2 cut(s) 2006, 2116
SmlI CTYRAG 2 cut(s) 1320, 2016
SmoI CTYRAG 2 cut(s) 1320, 2016
SphI GCATGC 1 cut(s) 1559
SsiI CCGC 3 cut(s) 81, 894, 1553
SspDI GGCGCC 2 cut(s) 1283, 2011
SspMI CTAG 2 cut(s) 576, 1212
SstI GAGCTC 3 cut(s) 19, 553, 1496
StyD4I CCNGG 5 cut(s) 350, 1440, 1441, 1675, 1729
StyI CCWWGG 2 cut(s) 941, 1860
TaaI ACNGT 5 cut(s) 72, 397, 1523, 1936, 2068
TaiI ACGT 2 cut(s) 107, 1751
TaqII GACCGA 1 cut(s) 282
TatI WGTACW 1 cut(s) 1841
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 6 cut(s) 208, 246, 428, 446, 566, 946
Tru1I TTAA 5 cut(s) 1046, 1767, 1920, 1998, 2058
Tru9I TTAA 5 cut(s) 1046, 1767, 1920, 1998, 2058
TscAI CASTG 3 cut(s) 268, 568, 1035
TseFI GTSAC 1 cut(s) 2062
Tsp45I GTSAC 1 cut(s) 2062
TspDTI ATGAA 6 cut(s) 308, 480, 761, 848, 1228, 1899
TspGWI ACGGA 6 cut(s) 27, 258, 298, 930, 1053, 2138
TspMI CCCGGG 1 cut(s) 1441
TspRI CASTG 3 cut(s) 268, 568, 1035
VpaK11BI GGWCC 2 cut(s) 476, 1120
XagI CCTNNNNNAGG 2 cut(s) 691, 915
XapI RAATTY 3 cut(s) 356, 520, 1682
XceI RCATGY 2 cut(s) 1514, 1559
XhoI CTCGAG 1 cut(s) 1320
XmaI CCCGGG 1 cut(s) 1441
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 2 cut(s) 576, 1212
ZrmI AGTACT 1 cut(s) 1843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.