MD02G1288900.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000257
Physical Location & Seq
Forward (+)
34531887 .. 34533035
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1288900.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGAAGGAAGTTGTCTCTGTTTTTCCGAGCACAACTTACAAACTCCAAACCACAAGATCTTGGGAGTTTTTGGGTTTCAATGAGAAACTCAAGCGTAATCCTACTGTCGAGAGCAATGTTATTGTTGGTGTCATCGATACCGGGATATGGCCTGAATCCGAAAGTTTTAGCGATGAAGGTTTTGGTCCAGCTCCAAAGAAGTGGAAAGGAGTTTGCAAAGGAGGCAAAAATTTCACTTGCAACAACAAAATCATTGGAGCTCGGTTTTACGTTGGTTCCTCAGCTAGAGATGAGATTGGTCATGGAACTCATACTGCCTCAACCGTAGCAGGAACTCCCGTAAAGGGTGTGAGCTTTTATGGACTGGCGCACGGAACTGCAACAGGAGGCGTTCCCTCTGCGAGAATTGCTGAATATAAAGTTTGCGATTCTGACGAGTGCGCTACAGACAAGATATTGGCTGCTTTTGATGATGCTATTGCTGATGGAGTTGACATCATCACAATTTCAATTGGAAACTCAATTGTAGTCCCTTTCGATCAGGATCCTATAGCAATCGGTGCTTTTCATGCACTGGAGAAAGGGATACTAACCTCTCAGTCTGCAGGGAATGACGGCCCTTATATGGGTACAATATCAAGTGTAGCACCATGGATTCTTACAGTTGCAGCAAGTAGCACAGACCGTCACATCATTGACAAGGTTGTTCTTGGAAACGGAAGGACACTTGTCGGGAATTCTGTGAACGCTTTTGATTTAAATGGAACAAGTTTTCCCTTGATAGAAGGCAAAGATGCATCGAGTGAATGCGGCGATTGTGATGAAGGTTGCCTAGACCCTAACTTAGTTAAGGGAAAGATTGTGGTATGTCGTTGGCCTATAGGCATTATCGAGGCTCGTCGAGTTGGAGCAATAGGTGCAGTAACTAACAGTACCATACTCGATGTTTCTCTCATTGTCCCACTACCTGCAGCATGTTTTAGAAGCAAAGATTTTGACGTTGTCAAGTCCTACGTCAACTCCACAGTCAAACCTCGAGCGAAACTACTAAAAAGTGAAGTCATAAAAGTCTTGCCGCACCTACAGTCGCTTCCTTCTCTTCACGGGGGCCCAATCGTATTTTATTTGAAATTATCAAGCCGGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

383

Amino Acids

40.52

Weight (kDa)

6.66

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 38 - 228 4.2e-20 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 976
AciI CCGC 2 cut(s) 810, 1076
AclWI GGATC 2 cut(s) 539, 552
AcsI RAATTY 2 cut(s) 229, 736
AfaI GTAC 2 cut(s) 631, 934
AfiI CCNNNNNNNGG 4 cut(s) 147, 344, 625, 626
AgsI TTSAA 3 cut(s) 79, 510, 1129
AloI GAACNNNNNNTCC 2 cut(s) 757, 789
AluBI AGCT 4 cut(s) 191, 260, 284, 354
AluI AGCT 4 cut(s) 191, 260, 284, 354
Alw21I GWGCWC 2 cut(s) 32, 262
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 2 cut(s) 539, 552
Ama87I CYCGRG 1 cut(s) 1035
AoxI GGCC 4 cut(s) 149, 616, 875, 1108
ApaI GGGCCC 1 cut(s) 1112
ApeKI GCWGC 3 cut(s) 461, 668, 971
ApoI RAATTY 2 cut(s) 229, 736
AspLEI GCGC 2 cut(s) 370, 443
AspS9I GGNCC 4 cut(s) 185, 617, 1108, 1109
AsuC2I CCSGG 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 1035
AvaII GGWCC 1 cut(s) 185
BaeGI GKGCMC 1 cut(s) 1112
BamHI GGATCC 1 cut(s) 544
BanII GRGCYC 2 cut(s) 262, 1112
Bbv12I GWGCWC 2 cut(s) 32, 262
BbvCI CCTCAGC 1 cut(s) 280
BbvI GCAGC 3 cut(s) 448, 680, 983
BccI CCATC 1 cut(s) 479
BceAI ACGGC 1 cut(s) 631
BciVI GTATCC 1 cut(s) 579
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 2 cut(s) 285, 833
BfmI CTRYAG 6 cut(s) 444, 549, 603, 879, 969, 1082
BfuAI ACCTGC 1 cut(s) 976
BfuI GTATCC 1 cut(s) 579
BglII AGATCT 1 cut(s) 56
BisI GCNGC 5 cut(s) 462, 669, 811, 972, 1076
BlsI GCNGC 5 cut(s) 463, 670, 812, 973, 1077
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 1 cut(s) 1035
BmgT120I GGNCC 4 cut(s) 185, 617, 1108, 1109
BmiI GGNNCC 4 cut(s) 277, 546, 1109, 1110
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 3 cut(s) 463, 784, 806
BoxI GACNNNNGTC 1 cut(s) 728
BpmI CTGGAG 1 cut(s) 596
Bpu10I CCTNAGC 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 74
BpuMI CCSGG 1 cut(s) 142
Bsa29I ATCGAT 1 cut(s) 135
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 1004, 1034
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 344, 625, 626
Bse1I ACTGG 2 cut(s) 369, 579
Bse3DI GCAATG 1 cut(s) 121
Bse8I GATNNNNATC 1 cut(s) 543
BseCI ATCGAT 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 650
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 4 cut(s) 147, 344, 625, 626
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 2 cut(s) 294, 611
BseNI ACTGG 2 cut(s) 369, 579
BseSI GKGCMC 1 cut(s) 1112
BseXI GCAGC 3 cut(s) 448, 680, 983
BsgI GTGCAG 1 cut(s) 939
BshFI GGCC 4 cut(s) 151, 618, 877, 1110
BshVI ATCGAT 1 cut(s) 135
BsiHKAI GWGCWC 2 cut(s) 32, 262
BsiHKCI CYCGRG 1 cut(s) 1035
BsiSI CCGG 2 cut(s) 141, 1141
BslFI GGGAC 2 cut(s) 515, 944
BslI CCNNNNNNNGG 4 cut(s) 147, 344, 625, 626
BsmAI GTCTC 1 cut(s) 19
BsmFI GGGAC 2 cut(s) 515, 944
BsmI GAATGC 1 cut(s) 812
BsnI GGCC 4 cut(s) 151, 618, 877, 1110
BsoBI CYCGRG 1 cut(s) 1035
Bsp120I GGGCCC 1 cut(s) 1108
Bsp1286I GDGCHC 3 cut(s) 32, 262, 1112
Bsp143I GATC 3 cut(s) 56, 538, 544
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 2 cut(s) 810, 1076
BspANI GGCC 4 cut(s) 151, 618, 877, 1110
BspCNI CTCAG 2 cut(s) 293, 610
BspDI ATCGAT 1 cut(s) 135
BspLI GGNNCC 4 cut(s) 277, 546, 1109, 1110
BspMAI CTGCAG 2 cut(s) 607, 973
BspMI ACCTGC 1 cut(s) 976
BspPI GGATC 2 cut(s) 539, 552
BsrDI GCAATG 1 cut(s) 121
BsrI ACTGG 2 cut(s) 369, 579
BssECI CCNNGG 1 cut(s) 650
BssMI GATC 3 cut(s) 56, 538, 544
BssT1I CCWWGG 1 cut(s) 650
Bst4CI ACNGT 7 cut(s) 106, 325, 664, 686, 932, 1027, 1086
Bst6I CTCTTC 1 cut(s) 1104
BstAPI GCANNNNNTGC 2 cut(s) 560, 917
BstDEI CTNAG 3 cut(s) 280, 597, 844
BstDSI CCRYGG 1 cut(s) 650
BstHHI GCGC 2 cut(s) 370, 443
BstKTI GATC 3 cut(s) 59, 541, 547
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 3 cut(s) 56, 538, 544
BstMWI GCNNNNNNNGC 5 cut(s) 222, 407, 560, 569, 917
BstNSI RCATGY 1 cut(s) 978
BstPAI GACNNNNGTC 1 cut(s) 728
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 6 cut(s) 444, 549, 603, 879, 969, 1082
BstSLI GKGCMC 1 cut(s) 1112
BstV1I GCAGC 3 cut(s) 448, 680, 983
BstX2I RGATCY 2 cut(s) 56, 544
BstXI CCANNNNNNTGG 1 cut(s) 201
BstYI RGATCY 2 cut(s) 56, 544
Bsu15I ATCGAT 1 cut(s) 135
BsuI GTATCC 1 cut(s) 579
BsuRI GGCC 4 cut(s) 151, 618, 877, 1110
BsuTUI ATCGAT 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 650
BtgZI GCGATG 1 cut(s) 186
BtsIMutI CAGTG 1 cut(s) 572
BveI ACCTGC 1 cut(s) 976
CfoI GCGC 2 cut(s) 370, 443
Cfr13I GGNCC 4 cut(s) 185, 617, 1108, 1109
ClaI ATCGAT 1 cut(s) 135
Csp6I GTAC 2 cut(s) 630, 933
CviAII CATG 4 cut(s) 302, 569, 651, 975
CviQI GTAC 2 cut(s) 630, 933
DdeI CTNAG 3 cut(s) 280, 597, 844
DpnI GATC 3 cut(s) 58, 540, 546
DpnII GATC 3 cut(s) 56, 538, 544
DraI TTTAAA 1 cut(s) 759
Eam1104I CTCTTC 1 cut(s) 1104
EarI CTCTTC 1 cut(s) 1104
Ecl136II GAGCTC 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 650
Eco24I GRGCYC 2 cut(s) 262, 1112
Eco47I GGWCC 1 cut(s) 185
Eco53kI GAGCTC 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 1035
EcoICRI GAGCTC 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 1108
EcoRI GAATTC 1 cut(s) 736
EcoT14I CCWWGG 1 cut(s) 650
EcoT22I ATGCAT 1 cut(s) 799
EcoT38I GRGCYC 2 cut(s) 262, 1112
ErhI CCWWGG 1 cut(s) 650
FaeI CATG 4 cut(s) 305, 572, 654, 978
FaqI GGGAC 2 cut(s) 515, 944
FatI CATG 4 cut(s) 301, 568, 650, 974
Fnu4HI GCNGC 5 cut(s) 462, 669, 811, 972, 1076
FriOI GRGCYC 2 cut(s) 262, 1112
Fsp4HI GCNGC 5 cut(s) 462, 669, 811, 972, 1076
FspBI CTAG 2 cut(s) 285, 833
GlaI GCGC 2 cut(s) 369, 442
GluI GCNGC 5 cut(s) 462, 669, 811, 972, 1076
GsuI CTGGAG 1 cut(s) 596
HaeIII GGCC 4 cut(s) 151, 618, 877, 1110
HapII CCGG 2 cut(s) 141, 1141
HhaI GCGC 2 cut(s) 370, 443
Hin1II CATG 4 cut(s) 305, 572, 654, 978
Hin6I GCGC 2 cut(s) 368, 441
HinP1I GCGC 2 cut(s) 368, 441
HincII GTYRAC 2 cut(s) 493, 1018
HindII GTYRAC 2 cut(s) 493, 1018
HinfI GANTC 3 cut(s) 155, 428, 655
HpaII CCGG 2 cut(s) 141, 1141
Hpy166II GTNNAC 3 cut(s) 493, 745, 1018
Hpy188I TCNGA 3 cut(s) 27, 160, 433
Hpy188III TCNNGA 3 cut(s) 109, 542, 733
Hpy8I GTNNAC 3 cut(s) 493, 745, 1018
Hpy99I CGWCG 1 cut(s) 903
HpyAV CCTTC 5 cut(s) 170, 714, 779, 818, 1104
HpyCH4III ACNGT 7 cut(s) 106, 325, 664, 686, 932, 1027, 1086
HpyCH4IV ACGT 3 cut(s) 270, 999, 1014
HpyCH4V TGCA 9 cut(s) 216, 240, 380, 572, 605, 668, 797, 920, 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 222, 407, 560, 569, 917
HpyF3I CTNAG 3 cut(s) 280, 597, 844
HpySE526I ACGT 3 cut(s) 270, 999, 1014
Hsp92II CATG 4 cut(s) 305, 572, 654, 978
HspAI GCGC 2 cut(s) 368, 441
Kzo9I GATC 3 cut(s) 56, 538, 544
LmnI GCTCC 3 cut(s) 196, 257, 908
Lsp1109I GCAGC 3 cut(s) 448, 680, 983
LweI GCATC 3 cut(s) 463, 784, 806
MaeI CTAG 2 cut(s) 285, 833
MaeII ACGT 3 cut(s) 270, 999, 1014
MaeIII GTNAC 2 cut(s) 686, 922
MalI GATC 3 cut(s) 58, 540, 546
MboI GATC 3 cut(s) 56, 538, 544
MboII GAAGA 1 cut(s) 1091
MfeI CAATTG 2 cut(s) 510, 522
MflI RGATCY 2 cut(s) 56, 544
MhlI GDGCHC 3 cut(s) 32, 262, 1112
MluCI AATT 7 cut(s) 229, 405, 504, 510, 522, 736, 1130
MmeI TCCRAC 1 cut(s) 886
MnlI CCTC 8 cut(s) 215, 289, 328, 380, 406, 604, 886, 1044
Mph1103I ATGCAT 1 cut(s) 799
MseI TTAA 2 cut(s) 758, 849
MspI CCGG 2 cut(s) 141, 1141
MspR9I CCNGG 1 cut(s) 142
MunI CAATTG 2 cut(s) 510, 522
Mva1269I GAATGC 1 cut(s) 812
MwoI GCNNNNNNNGC 5 cut(s) 222, 407, 560, 569, 917
NciI CCSGG 1 cut(s) 142
NcoI CCATGG 1 cut(s) 650
NdeII GATC 3 cut(s) 56, 538, 544
NlaIII CATG 4 cut(s) 305, 572, 654, 978
NlaIV GGNNCC 4 cut(s) 277, 546, 1109, 1110
NmuCI GTSAC 1 cut(s) 686
NsiI ATGCAT 1 cut(s) 799
NspI RCATGY 1 cut(s) 978
PaeR7I CTCGAG 1 cut(s) 1035
PctI GAATGC 1 cut(s) 812
PfeI GAWTC 3 cut(s) 155, 428, 655
PflFI GACNNNGTC 1 cut(s) 1001
PkrI GCNGC 5 cut(s) 463, 670, 812, 973, 1077
PshAI GACNNNNGTC 1 cut(s) 728
Psp124BI GAGCTC 1 cut(s) 262
PspN4I GGNNCC 4 cut(s) 277, 546, 1109, 1110
PspOMI GGGCCC 1 cut(s) 1108
PspPI GGNCC 4 cut(s) 185, 617, 1108, 1109
PspXI VCTCGAGB 1 cut(s) 1035
PstI CTGCAG 2 cut(s) 607, 973
PsuI RGATCY 2 cut(s) 56, 544
PsyI GACNNNGTC 1 cut(s) 1001
RsaI GTAC 2 cut(s) 631, 934
RsaNI GTAC 2 cut(s) 630, 933
SacI GAGCTC 1 cut(s) 262
SaqAI TTAA 2 cut(s) 758, 849
SatI GCNGC 5 cut(s) 462, 669, 811, 972, 1076
Sau3AI GATC 3 cut(s) 56, 538, 544
Sau96I GGNCC 4 cut(s) 185, 617, 1108, 1109
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 3 cut(s) 32, 262, 1112
SfaNI GCATC 3 cut(s) 463, 784, 806
SfcI CTRYAG 6 cut(s) 444, 549, 603, 879, 969, 1082
Sfr274I CTCGAG 1 cut(s) 1035
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 1035
SmiI ATTTAAAT 1 cut(s) 759
SmlI CTYRAG 2 cut(s) 89, 1035
SmoI CTYRAG 2 cut(s) 89, 1035
Sse9I AATT 7 cut(s) 229, 405, 504, 510, 522, 736, 1130
SsiI CCGC 2 cut(s) 810, 1076
SspMI CTAG 2 cut(s) 285, 833
SstI GAGCTC 1 cut(s) 262
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 650
SwaI ATTTAAAT 1 cut(s) 759
TaaI ACNGT 7 cut(s) 106, 325, 664, 686, 932, 1027, 1086
TaiI ACGT 3 cut(s) 273, 1002, 1017
TaqI TCGA 8 cut(s) 108, 135, 537, 800, 891, 901, 942, 1036
TasI AATT 7 cut(s) 229, 405, 504, 510, 522, 736, 1130
TauI GCSGC 2 cut(s) 813, 1078
TfiI GAWTC 3 cut(s) 155, 428, 655
Tru1I TTAA 2 cut(s) 758, 849
Tru9I TTAA 2 cut(s) 758, 849
TscAI CASTG 1 cut(s) 579
TseFI GTSAC 1 cut(s) 686
TseI GCWGC 3 cut(s) 461, 668, 971
Tsp45I GTSAC 1 cut(s) 686
TspDTI ATGAA 4 cut(s) 17, 189, 557, 837
TspGWI ACGGA 2 cut(s) 387, 732
TspRI CASTG 1 cut(s) 579
Tth111I GACNNNGTC 1 cut(s) 1001
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 2 cut(s) 229, 736
XceI RCATGY 1 cut(s) 978
XhoI CTCGAG 1 cut(s) 1035
XspI CTAG 2 cut(s) 285, 833
Zsp2I ATGCAT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.