Rh5AG439100

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000257
Physical Location & Seq
Forward (+)
75503699 .. 75512032
8334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG439100.1

Sequence Viewer

Length: 1026 bp
ATGGTCTATATTGTGTACTTGGGGTCACTTTCTGATGGGTTGTACTCGCCGTCATCTCACCATCTTAGTATTCTACAGAGAGTTGTCCAGGACAGTTCTCCTAAAAATGTTTTGGTAAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCCAGACTCACTGACCAGGAAAGAGAAAAACTTTCTAAAATGAAGGAAGTAGTCTCTGTTTTTCCAAGCAGAACATTGCAACTTCAAACAACAAGATCTTGGGACTTCATGGGATTCAATGAGAAAATTGGTCGAAATGCCACAGTTGAGAGTGATATTATTGTGGGTGTCATTGACAGTGGAATTTGGCCTGAATCTGAGAGTTTTAAGGATGAAGGTTTTGGTCCTCCACCCAAGAAGTGGAAAGGTGCTTGTGAAGGGGGCAAAAAATTTACTTGCAACAACAAGCTTATTGGAGCTCGGTATTACTCGTCAACAGAGTCTGCAAGGGATGACACAGGTCACGGAACCCACACTGCCTCAACGGCAGCAGGAAATGCTGTAAGAGATGTAAGCTTTTATGGACTAGCACAAGGTACTGCAAGAGGAGGAGTTCCCTCTGCAAGAATTGCAGCATACAAAGTCTGTACCCCGGGCACATTGTGTAGTGCACATGATACCCTGGCTGCTTTTGACGATGCAATTGCTGATGGAGTCGATATTATTACAGCTTCAGTTGCATTTAGTATGATACAAGAATTCGATGTGGATCCTCTTGCAATTGGTACTTTTCACGCAATGGAGAAAGGGATACTAACCTCACATGCTGCGGGCAACAATGGACCTTCCGGTGCTACTGTAACAAGTGTAGCACCTTGGGTGCTTACAGTTGCAGCAAGTAGCATGGACCGTCGGATCATTGACAAGGTTATCCTTGGAAATGGAAGGACAGTAATTGGGAATTCTGTGAACTCCTTCACATTAAATGGTACAAGTTTTCCTCTGATACATGGAAAAAATGCTTCAAAGAACTGCACAGAAACAATTGCGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

341

Amino Acids

36.36

Weight (kDa)

8.21

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 2 - 79 5.2e-18 Peptidase inhibitor I9
Peptidase_S8 PF00082 101 - 291 4.9e-20 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 390
AciI CCGC 2 cut(s) 800, 1019
AclWI GGATC 3 cut(s) 734, 747, 893
AcsI RAATTY 4 cut(s) 333, 419, 728, 931
AcuI CTGAAG 1 cut(s) 687
AdeI CACNNNGTG 1 cut(s) 633
AfaI GTAC 6 cut(s) 17, 44, 568, 619, 757, 961
AfiI CCNNNNNNNGG 1 cut(s) 390
AgsI TTSAA 4 cut(s) 139, 236, 268, 996
AjnI CCWGG 3 cut(s) 87, 165, 651
AluBI AGCT 4 cut(s) 439, 449, 546, 701
AluI AGCT 4 cut(s) 439, 449, 546, 701
Alw21I GWGCWC 2 cut(s) 451, 643
Alw26I GTCTC 1 cut(s) 208
Alw44I GTGCAC 1 cut(s) 639
AlwI GGATC 3 cut(s) 734, 747, 893
AlwNI CAGNNNCTG 1 cut(s) 473
Ama87I CYCGRG 1 cut(s) 622
AoxI GGCC 1 cut(s) 338
ApaLI GTGCAC 1 cut(s) 639
ApeKI GCWGC 6 cut(s) 149, 518, 602, 656, 797, 863
ApoI RAATTY 4 cut(s) 333, 419, 728, 931
Asp700I GAANNNNTTC 1 cut(s) 944
AspS9I GGNCC 3 cut(s) 374, 812, 877
AsuC2I CCSGG 2 cut(s) 623, 624
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 3 cut(s) 374, 812, 877
BaeGI GKGCMC 2 cut(s) 629, 643
BamHI GGATCC 1 cut(s) 739
BanII GRGCYC 1 cut(s) 451
Bbv12I GWGCWC 2 cut(s) 451, 643
BbvI GCAGC 6 cut(s) 136, 530, 614, 643, 784, 875
BccI CCATC 3 cut(s) 29, 69, 674
BceAI ACGGC 2 cut(s) 34, 531
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 3 cut(s) 89, 167, 653
BciVI GTATCC 1 cut(s) 774
BcnI CCSGG 2 cut(s) 623, 624
BcoDI GTCTC 1 cut(s) 208
BfaI CTAG 1 cut(s) 557
BfmI CTRYAG 1 cut(s) 74
BfuI GTATCC 1 cut(s) 774
BglI GCCNNNNNGGC 1 cut(s) 515
BglII AGATCT 1 cut(s) 245
BisI GCNGC 6 cut(s) 150, 519, 603, 657, 798, 864
BlsI GCNGC 6 cut(s) 151, 520, 604, 658, 799, 865
Bme1390I CCNGG 5 cut(s) 89, 167, 623, 624, 653
Bme18I GGWCC 3 cut(s) 374, 812, 877
BmeT110I CYCGRG 1 cut(s) 622
BmgT120I GGNCC 3 cut(s) 374, 812, 877
BmiI GGNNCC 2 cut(s) 499, 741
BmrFI CCNGG 5 cut(s) 89, 167, 623, 624, 653
BmsI GCATC 1 cut(s) 658
BoxI GACNNNNGTC 1 cut(s) 489
BpuMI CCSGG 2 cut(s) 623, 624
BsaBI GATNNNNATC 1 cut(s) 738
BsaJI CCNNGG 5 cut(s) 621, 622, 651, 845, 904
BsaWI WCCGGW 1 cut(s) 818
Bsc4I CCNNNNNNNGG 1 cut(s) 390
Bse3DI GCAATG 2 cut(s) 224, 774
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 3 cut(s) 89, 167, 653
BseDI CCNNGG 5 cut(s) 621, 622, 651, 845, 904
BseGI GGATG 2 cut(s) 367, 487
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 1 cut(s) 390
BseMI GCAATG 2 cut(s) 224, 774
BseMII CTCAG 1 cut(s) 339
BseRI GAGGAG 2 cut(s) 591, 594
BseSI GKGCMC 2 cut(s) 629, 643
BseXI GCAGC 6 cut(s) 136, 530, 614, 643, 784, 875
BsgI GTGCAG 1 cut(s) 988
BshFI GGCC 1 cut(s) 340
BsiHKAI GWGCWC 2 cut(s) 451, 643
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 2 cut(s) 623, 819
BslFI GGGAC 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 390
BsmAI GTCTC 1 cut(s) 208
BsmFI GGGAC 1 cut(s) 266
BsnI GGCC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 622
Bsp1286I GDGCHC 3 cut(s) 451, 629, 643
Bsp143I GATC 3 cut(s) 245, 739, 885
BspACI CCGC 2 cut(s) 800, 1019
BspANI GGCC 1 cut(s) 340
BspCNI CTCAG 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 499, 741
BspPI GGATC 3 cut(s) 734, 747, 893
BsrDI GCAATG 2 cut(s) 224, 774
BssECI CCNNGG 5 cut(s) 621, 622, 651, 845, 904
BssMI GATC 3 cut(s) 245, 739, 885
BssT1I CCWWGG 2 cut(s) 845, 904
Bst2UI CCWGG 3 cut(s) 89, 167, 653
Bst4CI ACNGT 7 cut(s) 95, 295, 329, 829, 859, 881, 922
BstAPI GCANNNNNTGC 2 cut(s) 527, 599
BstC8I GCNNGC 1 cut(s) 802
BstDEI CTNAG 2 cut(s) 65, 348
BstF5I GGATG 2 cut(s) 367, 487
BstKTI GATC 3 cut(s) 248, 742, 888
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 3 cut(s) 245, 739, 885
BstMWI GCNNNNNNNGC 4 cut(s) 515, 527, 599, 707
BstNI CCWGG 3 cut(s) 89, 167, 653
BstNSI RCATGY 1 cut(s) 797
BstPAI GACNNNNGTC 1 cut(s) 489
BstSCI CCNGG 5 cut(s) 87, 165, 621, 622, 651
BstSFI CTRYAG 1 cut(s) 74
BstSLI GKGCMC 2 cut(s) 629, 643
BstV1I GCAGC 6 cut(s) 136, 530, 614, 643, 784, 875
BstX2I RGATCY 2 cut(s) 245, 739
BstYI RGATCY 2 cut(s) 245, 739
BsuI GTATCC 1 cut(s) 774
BsuRI GGCC 1 cut(s) 340
BtsCI GGATG 2 cut(s) 367, 487
BtsI GCAGTG 1 cut(s) 504
BtsIMutI CAGTG 3 cut(s) 159, 334, 504
Cac8I GCNNGC 1 cut(s) 802
CaiI CAGNNNCTG 1 cut(s) 473
Cfr13I GGNCC 3 cut(s) 374, 812, 877
Cfr9I CCCGGG 1 cut(s) 622
Csp6I GTAC 6 cut(s) 16, 43, 567, 618, 756, 960
CviAII CATG 5 cut(s) 259, 644, 794, 874, 980
CviJI RGCY 6 cut(s) 340, 439, 449, 546, 656, 701
CviKI_1 RGCY 6 cut(s) 340, 439, 449, 546, 656, 701
CviQI GTAC 6 cut(s) 16, 43, 567, 618, 756, 960
DdeI CTNAG 2 cut(s) 65, 348
DpnI GATC 3 cut(s) 247, 741, 887
DpnII GATC 3 cut(s) 245, 739, 885
DraIII CACNNNGTG 1 cut(s) 633
Ecl136II GAGCTC 1 cut(s) 449
Eco130I CCWWGG 2 cut(s) 845, 904
Eco24I GRGCYC 1 cut(s) 451
Eco47I GGWCC 3 cut(s) 374, 812, 877
Eco53kI GAGCTC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 687
Eco88I CYCGRG 1 cut(s) 622
EcoICRI GAGCTC 1 cut(s) 449
EcoRI GAATTC 2 cut(s) 728, 931
EcoRII CCWGG 3 cut(s) 87, 165, 651
EcoT14I CCWWGG 2 cut(s) 845, 904
EcoT38I GRGCYC 1 cut(s) 451
ErhI CCWWGG 2 cut(s) 845, 904
FaeI CATG 5 cut(s) 262, 647, 797, 877, 983
FaiI YATR 9 cut(s) 9, 260, 552, 607, 645, 719, 795, 875, 981
FaqI GGGAC 1 cut(s) 266
FatI CATG 5 cut(s) 258, 643, 793, 873, 979
FauI CCCGC 2 cut(s) 793, 1012
Fnu4HI GCNGC 6 cut(s) 150, 519, 603, 657, 798, 864
FokI GGATG 2 cut(s) 374, 494
FriOI GRGCYC 1 cut(s) 451
Fsp4HI GCNGC 6 cut(s) 150, 519, 603, 657, 798, 864
FspBI CTAG 1 cut(s) 557
GluI GCNGC 6 cut(s) 150, 519, 603, 657, 798, 864
HaeIII GGCC 1 cut(s) 340
HapII CCGG 2 cut(s) 623, 819
Hin1II CATG 5 cut(s) 262, 647, 797, 877, 983
HincII GTYRAC 1 cut(s) 465
HindII GTYRAC 1 cut(s) 465
HindIII AAGCTT 2 cut(s) 437, 544
HinfI GANTC 5 cut(s) 156, 264, 344, 470, 684
HpaII CCGG 2 cut(s) 623, 819
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 4 cut(s) 16, 465, 641, 940
Hpy188I TCNGA 4 cut(s) 34, 349, 885, 975
Hpy8I GTNNAC 4 cut(s) 16, 465, 641, 940
Hpy99I CGWCG 1 cut(s) 885
HpyAV CCTTC 6 cut(s) 187, 359, 401, 825, 909, 955
HpyCH4III ACNGT 7 cut(s) 95, 295, 329, 829, 859, 881, 922
HpyF10VI GCNNNNNNNGC 4 cut(s) 515, 527, 599, 707
HpyF3I CTNAG 2 cut(s) 65, 348
Hsp92II CATG 5 cut(s) 262, 647, 797, 877, 983
Kzo9I GATC 3 cut(s) 245, 739, 885
LmnI GCTCC 1 cut(s) 446
Lsp1109I GCAGC 6 cut(s) 136, 530, 614, 643, 784, 875
LweI GCATC 1 cut(s) 658
MaeI CTAG 1 cut(s) 557
MaeIII GTNAC 4 cut(s) 24, 122, 491, 829
MalI GATC 3 cut(s) 247, 741, 887
MboI GATC 3 cut(s) 245, 739, 885
MfeI CAATTG 3 cut(s) 672, 750, 1014
MflI RGATCY 2 cut(s) 245, 739
MhlI GDGCHC 3 cut(s) 451, 629, 643
MlyI GAGTC 3 cut(s) 150, 479, 693
MmeI TCCRAC 1 cut(s) 863
MnlI CCTC 8 cut(s) 387, 520, 569, 572, 598, 753, 799, 981
MroXI GAANNNNTTC 1 cut(s) 944
MseI TTAA 2 cut(s) 357, 953
MspI CCGG 2 cut(s) 623, 819
MspR9I CCNGG 5 cut(s) 89, 167, 623, 624, 653
MunI CAATTG 3 cut(s) 672, 750, 1014
MvaI CCWGG 3 cut(s) 89, 167, 653
MwoI GCNNNNNNNGC 4 cut(s) 515, 527, 599, 707
NciI CCSGG 2 cut(s) 623, 624
NdeII GATC 3 cut(s) 245, 739, 885
NlaIII CATG 5 cut(s) 262, 647, 797, 877, 983
NlaIV GGNNCC 2 cut(s) 499, 741
NmuCI GTSAC 2 cut(s) 24, 491
NspI RCATGY 1 cut(s) 797
PdmI GAANNNNTTC 1 cut(s) 944
PfeI GAWTC 2 cut(s) 264, 344
PflMI CCANNNNNTGG 1 cut(s) 390
PfoI TCCNGGA 1 cut(s) 87
PkrI GCNGC 6 cut(s) 151, 520, 604, 658, 799, 865
PleI GAGTC 3 cut(s) 150, 478, 692
PpsI GAGTC 3 cut(s) 150, 478, 692
PshAI GACNNNNGTC 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 451
Psp6I CCWGG 3 cut(s) 87, 165, 651
PspGI CCWGG 3 cut(s) 87, 165, 651
PspN4I GGNNCC 2 cut(s) 499, 741
PspPI GGNCC 3 cut(s) 374, 812, 877
PstNI CAGNNNCTG 1 cut(s) 473
PsuI RGATCY 2 cut(s) 245, 739
RsaI GTAC 6 cut(s) 17, 44, 568, 619, 757, 961
RsaNI GTAC 6 cut(s) 16, 43, 567, 618, 756, 960
SacI GAGCTC 1 cut(s) 451
SaqAI TTAA 2 cut(s) 357, 953
SatI GCNGC 6 cut(s) 150, 519, 603, 657, 798, 864
Sau3AI GATC 3 cut(s) 245, 739, 885
Sau96I GGNCC 3 cut(s) 374, 812, 877
SchI GAGTC 3 cut(s) 150, 479, 693
ScrFI CCNGG 5 cut(s) 89, 167, 623, 624, 653
SduI GDGCHC 3 cut(s) 451, 629, 643
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 1 cut(s) 74
SinI GGWCC 3 cut(s) 374, 812, 877
SmaI CCCGGG 1 cut(s) 624
SsiI CCGC 2 cut(s) 800, 1019
SspMI CTAG 1 cut(s) 557
SstI GAGCTC 1 cut(s) 451
StyD4I CCNGG 5 cut(s) 87, 165, 621, 622, 651
StyI CCWWGG 2 cut(s) 845, 904
TaaI ACNGT 7 cut(s) 95, 295, 329, 829, 859, 881, 922
TaqI TCGA 3 cut(s) 283, 687, 732
TatI WGTACW 2 cut(s) 15, 42
TfiI GAWTC 2 cut(s) 264, 344
Tru1I TTAA 2 cut(s) 357, 953
Tru9I TTAA 2 cut(s) 357, 953
TscAI CASTG 3 cut(s) 166, 334, 511
TseFI GTSAC 2 cut(s) 24, 491
TseI GCWGC 6 cut(s) 149, 518, 602, 656, 797, 863
Tsp45I GTSAC 2 cut(s) 24, 491
TspDTI ATGAA 3 cut(s) 206, 247, 378
TspGWI ACGGA 1 cut(s) 510
TspMI CCCGGG 1 cut(s) 622
TspRI CASTG 3 cut(s) 166, 334, 511
Van91I CCANNNNNTGG 1 cut(s) 390
VneI GTGCAC 1 cut(s) 639
VpaK11BI GGWCC 3 cut(s) 374, 812, 877
XapI RAATTY 4 cut(s) 333, 419, 728, 931
XceI RCATGY 1 cut(s) 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 387
XmaI CCCGGG 1 cut(s) 622
XmnI GAANNNNTTC 1 cut(s) 944
XspI CTAG 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.