Rw1G004750

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000257
Physical Location & Seq
Reverse (-)
9100889 .. 9110067
9179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004750.1

Sequence Viewer

Length: 1551 bp
ATGTCTGGTGTGTACTTGATACTTTTAGAAAATGAAAAGGTCCATATTGTGTACCTGGAATCACTTCCTGATGATGAGGTCTATTCATACATGTCTCATCACCTTGGTATACTAGAAAGAGTACGTTGTTGGGAGAAACTCTGCTGCAAAGTTCTTGACTATAAGAGTTACCAAAGGAGCTTCAATGGATTTGCTGCAAAGCTCAGTGATCATGAAAAAGAAAAGATTGCTAACATGGGGGAGGTGATCTCCGTCTTTCCTAGCAGAACGTTCCAACTTCAAACGACGAGGTCATGGGACTTTATGGGTCTCAAGCTGACAAGTCCTCGAAAAGTCACTGCTGAGAGTAATGTTATTGTTGGTGTGATTGACACTGGAATTGACCCTGATTCGGAGAGCTTTAGAGATGAAGGTTTTGGTGCTGCTCCTATGAAGTGGAAAGGTGTTTGTGAAGGTGGAGAAAATTTCAGTTGCAACAAGAAGATAATTGGAGCTCGTTTTTACGAACCAGCAGAGTCTGCAAAGGATGAAGACGGCCATGGAACCCACACTGCCTCAACGGCAGCAGGGAATGAGGTAAGGGATGTGAGCTTTTACGGATTAGAACCAGGTACAGCAAGAGGAGGGGTTCCCTCAGCGAGAATTGCTGCGTATAAAGTCTGTGGGCCTACAGGGTGCTCTTCAGAGGCTATATTGGCTGCTTTTGACGATGCAATTGCTGATGGAGTTGACATTGTGTCAGTCTCACTTGGAAGTGCAAGTCCCCTTCCTTTTGATAATGATCCTATAGCAATTGGTTCTTATCATGCAATGACGAGAGGGATATTGACATCAAACTCTGCAGGCAACAGTGGTCCTGGAGCTGGTACTGTAACAAGTGTAGCGCCGTGGATGCTTGCAGTTGCAGCAAGTAGCAAAGATCGTCGGATCATTGACAAGTTCCACCCTGATTGGTCTCCATCTGCCATTAAATCTGCTCTTATGACTACAGCTTGGCCTATGAATCATACAACCAACAGCTCATGCCCGGGTGAATTTGCATATGGATCCGGTCATATCAATCCTCTAAAAGCTATTGACCCAGGGCTTGTGTATGATGCTTCTGCGGAGGATTATATTAAGTTACTCTGCTTGGACATGGACGAGGCGAGAGTCAGACTTATATCAGGAGATAACAGCACTTGTCCTACAGCTTCTGAACAAAGATCGGCAAAGGATCTCAACTACCCTTCAATATCAGCTATTGTTACACCAATGACACGTTTTACGGTGACAATACGTAGAAGAGTTACAAATGTTGGCCTTGCAAATTCCACTTACAAGGCCGAAATCATGCCAAACTCTCAAGTTGAAATCAAAGTGGAGCCTCAAGTTCTTTCCTTCAAGTCCGTGAACCAGGAGAAGACTTTTGATTTGACCATTGTTGGAAGAGGGTTGCCAGATGGATCACATATGTCTTCGTCCTTGGTTTGGTCCGACGGAACTCATAAGGTTAAAAGTCCAGTTCTAGTGCAAAGCTTATCAGCAGCAGCATCATCACGGTTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

516

Amino Acids

55.72

Weight (kDa)

5.85

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 15 - 94 1.7e-09 Peptidase inhibitor I9
Peptidase_S8 PF00082 116 - 303 7.9e-23 Subtilase family
fn3_6 PF17766 406 - 504 4.9e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 109
AciI CCGC 1 cut(s) 1106
AclI AACGTT 1 cut(s) 269
AclWI GGATC 6 cut(s) 776, 935, 1041, 1054, 1224, 1453
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 3 cut(s) 463, 1034, 1309
AcuI CTGAAG 1 cut(s) 666
AfaI GTAC 5 cut(s) 14, 53, 123, 613, 868
AfiI CCNNNNNNNGG 3 cut(s) 391, 863, 1470
AflIII ACRYGT 2 cut(s) 90, 1259
AgsI TTSAA 5 cut(s) 184, 281, 1233, 1352, 1384
AhdI GACNNNNNGTC 1 cut(s) 736
AjnI CCWGG 5 cut(s) 54, 607, 856, 1081, 1395
Alw21I GWGCWC 2 cut(s) 496, 680
Alw26I GTCTC 4 cut(s) 99, 314, 748, 960
AlwI GGATC 6 cut(s) 776, 935, 1041, 1054, 1224, 1453
AlwNI CAGNNNCTG 2 cut(s) 518, 1196
Ama87I CYCGRG 1 cut(s) 1027
AoxI GGCC 5 cut(s) 535, 665, 995, 1300, 1323
ApeKI GCWGC 9 cut(s) 144, 194, 422, 563, 647, 698, 905, 1526, 1529
ApoI RAATTY 3 cut(s) 463, 1034, 1309
ArsI GACNNNNNNTTYG 2 cut(s) 1396, 1428
Asp700I GAANNNNTTC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 886
AspS9I GGNCC 4 cut(s) 40, 665, 854, 1473
AsuC2I CCSGG 2 cut(s) 1028, 1029
AsuHPI GGTGA 4 cut(s) 92, 256, 1043, 1282
AvaI CYCGRG 1 cut(s) 1027
AvaII GGWCC 3 cut(s) 40, 854, 1473
BamHI GGATCC 1 cut(s) 1046
BanII GRGCYC 1 cut(s) 496
BbsI GAAGAC 3 cut(s) 537, 1409, 1449
Bbv12I GWGCWC 2 cut(s) 496, 680
BbvCI CCTCAGC 1 cut(s) 634
BbvI GCAGC 9 cut(s) 131, 181, 409, 575, 634, 685, 917, 1538, 1541
BccI CCATC 3 cut(s) 716, 967, 1436
BceAI ACGGC 3 cut(s) 550, 576, 871
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 5 cut(s) 56, 609, 858, 1083, 1397
BclI TGATCA 1 cut(s) 208
BcnI CCSGG 2 cut(s) 1028, 1029
BcoDI GTCTC 4 cut(s) 99, 314, 748, 960
BfaI CTAG 3 cut(s) 113, 261, 1508
BfmI CTRYAG 5 cut(s) 669, 786, 840, 987, 1188
BfoI RGCGCY 1 cut(s) 887
BglI GCCNNNNNGGC 1 cut(s) 560
BisI GCNGC 9 cut(s) 145, 195, 423, 564, 648, 699, 906, 1527, 1530
BlsI GCNGC 9 cut(s) 146, 196, 424, 565, 649, 700, 907, 1528, 1531
Bme1390I CCNGG 7 cut(s) 56, 609, 858, 1028, 1029, 1083, 1397
Bme18I GGWCC 3 cut(s) 40, 854, 1473
BmeRI GACNNNNNGTC 1 cut(s) 736
BmeT110I CYCGRG 1 cut(s) 1027
BmgT120I GGNCC 4 cut(s) 40, 665, 854, 1473
BmiI GGNNCC 4 cut(s) 544, 630, 1048, 1365
BmrFI CCNGG 7 cut(s) 56, 609, 858, 1028, 1029, 1083, 1397
BmsI GCATC 4 cut(s) 700, 882, 1087, 1541
BpiI GAAGAC 3 cut(s) 537, 1409, 1449
BplI GAGNNNNNCTC 2 cut(s) 233, 265
BpmI CTGGAG 1 cut(s) 879
Bpu10I CCTNAGC 1 cut(s) 634
BpuEI CTTGAG 3 cut(s) 296, 1329, 1353
BpuMI CCSGG 2 cut(s) 1028, 1029
BsaAI YACGTR 1 cut(s) 1280
BsaBI GATNNNNATC 1 cut(s) 780
BsaI GGTCTC 2 cut(s) 314, 960
BsaJI CCNNGG 7 cut(s) 103, 538, 887, 1027, 1081, 1082, 1464
BsaWI WCCGGW 1 cut(s) 1049
Bsc4I CCNNNNNNNGG 3 cut(s) 391, 863, 1470
Bse1I ACTGG 2 cut(s) 379, 1502
Bse3DI GCAATG 1 cut(s) 816
Bse8I GATNNNNATC 1 cut(s) 780
BseBI CCWGG 5 cut(s) 56, 609, 858, 1083, 1397
BseDI CCNNGG 7 cut(s) 103, 538, 887, 1027, 1081, 1082, 1464
BseGI GGATG 3 cut(s) 532, 589, 897
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 3 cut(s) 391, 863, 1470
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 3 cut(s) 217, 333, 648
BseNI ACTGG 2 cut(s) 379, 1502
BseRI GAGGAG 1 cut(s) 636
BseXI GCAGC 9 cut(s) 131, 181, 409, 575, 634, 685, 917, 1538, 1541
BshFI GGCC 5 cut(s) 537, 667, 997, 1302, 1325
BsiHKAI GWGCWC 2 cut(s) 496, 680
BsiHKCI CYCGRG 1 cut(s) 1027
BsiSI CCGG 2 cut(s) 1028, 1050
BslFI GGGAC 2 cut(s) 311, 747
BslI CCNNNNNNNGG 3 cut(s) 391, 863, 1470
BsmAI GTCTC 4 cut(s) 99, 314, 748, 960
BsmFI GGGAC 2 cut(s) 311, 747
BsnI GGCC 5 cut(s) 537, 667, 997, 1302, 1325
Bso31I GGTCTC 2 cut(s) 314, 960
BsoBI CYCGRG 1 cut(s) 1027
Bsp1286I GDGCHC 2 cut(s) 496, 680
Bsp143I GATC 9 cut(s) 208, 246, 781, 919, 927, 1046, 1205, 1216, 1445
Bsp19I CCATGG 1 cut(s) 538
BspACI CCGC 1 cut(s) 1106
BspANI GGCC 5 cut(s) 537, 667, 997, 1302, 1325
BspCNI CTCAG 3 cut(s) 216, 334, 647
BspHI TCATGA 1 cut(s) 211
BspLI GGNNCC 4 cut(s) 544, 630, 1048, 1365
BspMAI CTGCAG 1 cut(s) 844
BspPI GGATC 6 cut(s) 776, 935, 1041, 1054, 1224, 1453
BspQI GCTCTTC 1 cut(s) 685
BspTNI GGTCTC 2 cut(s) 314, 960
BsrDI GCAATG 1 cut(s) 816
BsrI ACTGG 2 cut(s) 379, 1502
BssECI CCNNGG 7 cut(s) 103, 538, 887, 1027, 1081, 1082, 1464
BssMI GATC 9 cut(s) 208, 246, 781, 919, 927, 1046, 1205, 1216, 1445
BssNAI GTATAC 1 cut(s) 110
BssT1I CCWWGG 3 cut(s) 103, 538, 1464
Bst1107I GTATAC 1 cut(s) 110
Bst2UI CCWGG 5 cut(s) 56, 609, 858, 1083, 1397
Bst4CI ACNGT 4 cut(s) 851, 871, 1270, 1542
Bst6I CTCTTC 3 cut(s) 685, 1279, 1423
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 1280
BstC8I GCNNGC 2 cut(s) 844, 897
BstDEI CTNAG 3 cut(s) 203, 342, 634
BstDSI CCRYGG 2 cut(s) 538, 887
BstF5I GGATG 3 cut(s) 532, 589, 897
BstH2I RGCGCY 1 cut(s) 887
BstHHI GCGC 1 cut(s) 886
BstKTI GATC 9 cut(s) 211, 249, 784, 922, 930, 1049, 1208, 1219, 1448
BstMAI GTCTC 4 cut(s) 99, 314, 748, 960
BstMBI GATC 9 cut(s) 208, 246, 781, 919, 927, 1046, 1205, 1216, 1445
BstMWI GCNNNNNNNGC 6 cut(s) 518, 560, 644, 695, 892, 905
BstNI CCWGG 5 cut(s) 56, 609, 858, 1083, 1397
BstNSI RCATGY 1 cut(s) 94
BstSCI CCNGG 7 cut(s) 54, 607, 856, 1026, 1027, 1081, 1395
BstSFI CTRYAG 5 cut(s) 669, 786, 840, 987, 1188
BstSNI TACGTA 1 cut(s) 1280
BstV1I GCAGC 9 cut(s) 131, 181, 409, 575, 634, 685, 917, 1538, 1541
BstV2I GAAGAC 3 cut(s) 537, 1409, 1449
BstX2I RGATCY 2 cut(s) 1046, 1216
BstYI RGATCY 2 cut(s) 1046, 1216
BstZ17I GTATAC 1 cut(s) 110
BsuRI GGCC 5 cut(s) 537, 667, 997, 1302, 1325
BtgI CCRYGG 2 cut(s) 538, 887
BtsCI GGATG 3 cut(s) 532, 589, 897
BtsI GCAGTG 2 cut(s) 336, 549
BtsIMutI CAGTG 5 cut(s) 211, 336, 372, 549, 856
Cac8I GCNNGC 2 cut(s) 844, 897
CaiI CAGNNNCTG 2 cut(s) 518, 1196
CciI TCATGA 1 cut(s) 211
CfoI GCGC 1 cut(s) 886
Cfr13I GGNCC 4 cut(s) 40, 665, 854, 1473
Cfr9I CCCGGG 1 cut(s) 1027
CsiI ACCWGGT 1 cut(s) 607
Csp6I GTAC 5 cut(s) 13, 52, 122, 612, 867
CspCI CAANNNNNGTGG 2 cut(s) 932, 967
CviAII CATG 9 cut(s) 91, 212, 235, 294, 539, 806, 1023, 1138, 1333
CviQI GTAC 5 cut(s) 13, 52, 122, 612, 867
DdeI CTNAG 3 cut(s) 203, 342, 634
DpnI GATC 9 cut(s) 210, 248, 783, 921, 929, 1048, 1207, 1218, 1447
DpnII GATC 9 cut(s) 208, 246, 781, 919, 927, 1046, 1205, 1216, 1445
DriI GACNNNNNGTC 1 cut(s) 736
EaeI YGGCCR 1 cut(s) 535
Eam1104I CTCTTC 3 cut(s) 685, 1279, 1423
Eam1105I GACNNNNNGTC 1 cut(s) 736
EarI CTCTTC 3 cut(s) 685, 1279, 1423
Ecl136II GAGCTC 1 cut(s) 494
Eco105I TACGTA 1 cut(s) 1280
Eco130I CCWWGG 3 cut(s) 103, 538, 1464
Eco24I GRGCYC 1 cut(s) 496
Eco31I GGTCTC 2 cut(s) 314, 960
Eco47I GGWCC 3 cut(s) 40, 854, 1473
Eco53kI GAGCTC 1 cut(s) 494
Eco57I CTGAAG 1 cut(s) 666
Eco88I CYCGRG 1 cut(s) 1027
EcoICRI GAGCTC 1 cut(s) 494
EcoRII CCWGG 5 cut(s) 54, 607, 856, 1081, 1395
EcoT14I CCWWGG 3 cut(s) 103, 538, 1464
EcoT38I GRGCYC 1 cut(s) 496
ErhI CCWWGG 3 cut(s) 103, 538, 1464
FaeI CATG 9 cut(s) 94, 215, 238, 297, 542, 809, 1026, 1141, 1336
FaqI GGGAC 2 cut(s) 311, 747
FatI CATG 9 cut(s) 90, 211, 234, 293, 538, 805, 1022, 1137, 1332
FauNDI CATATG 2 cut(s) 1042, 1452
FbaI TGATCA 1 cut(s) 208
FblI GTMKAC 1 cut(s) 109
Fnu4HI GCNGC 9 cut(s) 145, 195, 423, 564, 648, 699, 906, 1527, 1530
FokI GGATG 3 cut(s) 539, 596, 904
FriOI GRGCYC 1 cut(s) 496
Fsp4HI GCNGC 9 cut(s) 145, 195, 423, 564, 648, 699, 906, 1527, 1530
FspBI CTAG 3 cut(s) 113, 261, 1508
GlaI GCGC 1 cut(s) 885
GluI GCNGC 9 cut(s) 145, 195, 423, 564, 648, 699, 906, 1527, 1530
GsuI CTGGAG 1 cut(s) 879
HaeII RGCGCY 1 cut(s) 887
HaeIII GGCC 5 cut(s) 537, 667, 997, 1302, 1325
HapII CCGG 2 cut(s) 1028, 1050
HhaI GCGC 1 cut(s) 886
Hin1II CATG 9 cut(s) 94, 215, 238, 297, 542, 809, 1026, 1141, 1336
Hin6I GCGC 1 cut(s) 884
HinP1I GCGC 1 cut(s) 884
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 1 cut(s) 1516
HinfI GANTC 5 cut(s) 59, 389, 515, 1003, 1152
HpaII CCGG 2 cut(s) 1028, 1050
HphI GGTGA 4 cut(s) 92, 256, 1043, 1282
Hpy166II GTNNAC 5 cut(s) 13, 52, 110, 730, 1393
Hpy188I TCNGA 6 cut(s) 394, 685, 927, 1157, 1198, 1477
Hpy188III TCNNGA 4 cut(s) 68, 155, 212, 1167
Hpy8I GTNNAC 5 cut(s) 13, 52, 110, 730, 1393
Hpy99I CGWCG 3 cut(s) 289, 927, 1481
HpyAV CCTTC 5 cut(s) 404, 446, 776, 1239, 1390
HpyCH4III ACNGT 4 cut(s) 851, 871, 1270, 1542
HpyCH4IV ACGT 4 cut(s) 124, 269, 1261, 1279
HpyF10VI GCNNNNNNNGC 6 cut(s) 518, 560, 644, 695, 892, 905
HpyF3I CTNAG 3 cut(s) 203, 342, 634
HpySE526I ACGT 4 cut(s) 124, 269, 1261, 1279
Hsp92II CATG 9 cut(s) 94, 215, 238, 297, 542, 809, 1026, 1141, 1336
HspAI GCGC 1 cut(s) 884
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 9 cut(s) 208, 246, 781, 919, 927, 1046, 1205, 1216, 1445
LguI GCTCTTC 1 cut(s) 685
LmnI GCTCC 5 cut(s) 177, 430, 491, 860, 1363
Lsp1109I GCAGC 9 cut(s) 131, 181, 409, 575, 634, 685, 917, 1538, 1541
LweI GCATC 4 cut(s) 700, 882, 1087, 1541
MabI ACCWGGT 1 cut(s) 607
MaeI CTAG 3 cut(s) 113, 261, 1508
MaeII ACGT 4 cut(s) 124, 269, 1261, 1279
MaeIII GTNAC 7 cut(s) 167, 334, 871, 1122, 1246, 1270, 1288
MalI GATC 9 cut(s) 210, 248, 783, 921, 929, 1048, 1207, 1218, 1447
MboI GATC 9 cut(s) 208, 246, 781, 919, 927, 1046, 1205, 1216, 1445
MboII GAAGA 7 cut(s) 493, 542, 672, 1296, 1414, 1440, 1449
MfeI CAATTG 2 cut(s) 714, 792
MflI RGATCY 2 cut(s) 1046, 1216
MhlI GDGCHC 2 cut(s) 496, 680
MluCI AATT 8 cut(s) 378, 463, 486, 642, 714, 792, 1034, 1309
MlyI GAGTC 2 cut(s) 524, 1161
MmeI TCCRAC 4 cut(s) 298, 905, 1405, 1500
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 4 cut(s) 969, 1119, 1494, 1549
MspI CCGG 2 cut(s) 1028, 1050
MspR9I CCNGG 7 cut(s) 56, 609, 858, 1028, 1029, 1083, 1397
MunI CAATTG 2 cut(s) 714, 792
MvaI CCWGG 5 cut(s) 56, 609, 858, 1083, 1397
MwoI GCNNNNNNNGC 6 cut(s) 518, 560, 644, 695, 892, 905
NciI CCSGG 2 cut(s) 1028, 1029
NcoI CCATGG 1 cut(s) 538
NdeI CATATG 2 cut(s) 1042, 1452
NdeII GATC 9 cut(s) 208, 246, 781, 919, 927, 1046, 1205, 1216, 1445
NlaIII CATG 9 cut(s) 94, 215, 238, 297, 542, 809, 1026, 1141, 1336
NlaIV GGNNCC 4 cut(s) 544, 630, 1048, 1365
NmuCI GTSAC 2 cut(s) 334, 1270
NspI RCATGY 1 cut(s) 94
PagI TCATGA 1 cut(s) 211
PasI CCCWGGG 1 cut(s) 1082
PciI ACATGT 1 cut(s) 90
PciSI GCTCTTC 1 cut(s) 685
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 3 cut(s) 59, 389, 1003
PflFI GACNNNGTC 1 cut(s) 289
PfoI TCCNGGA 1 cut(s) 856
PkrI GCNGC 9 cut(s) 146, 196, 424, 565, 649, 700, 907, 1528, 1531
PleI GAGTC 2 cut(s) 523, 1160
PpsI GAGTC 2 cut(s) 523, 1160
Ppu21I YACGTR 1 cut(s) 1280
PscI ACATGT 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 496
Psp1406I AACGTT 1 cut(s) 269
Psp6I CCWGG 5 cut(s) 54, 607, 856, 1081, 1395
PspGI CCWGG 5 cut(s) 54, 607, 856, 1081, 1395
PspN4I GGNNCC 4 cut(s) 544, 630, 1048, 1365
PspPI GGNCC 4 cut(s) 40, 665, 854, 1473
PstI CTGCAG 1 cut(s) 844
PstNI CAGNNNCTG 2 cut(s) 518, 1196
PsuI RGATCY 2 cut(s) 1046, 1216
PsyI GACNNNGTC 1 cut(s) 289
RsaI GTAC 5 cut(s) 14, 53, 123, 613, 868
RsaNI GTAC 5 cut(s) 13, 52, 122, 612, 867
SacI GAGCTC 1 cut(s) 496
SapI GCTCTTC 1 cut(s) 685
SaqAI TTAA 4 cut(s) 969, 1119, 1494, 1549
SatI GCNGC 9 cut(s) 145, 195, 423, 564, 648, 699, 906, 1527, 1530
Sau3AI GATC 9 cut(s) 208, 246, 781, 919, 927, 1046, 1205, 1216, 1445
Sau96I GGNCC 4 cut(s) 40, 665, 854, 1473
SchI GAGTC 2 cut(s) 524, 1161
ScrFI CCNGG 7 cut(s) 56, 609, 858, 1028, 1029, 1083, 1397
SduI GDGCHC 2 cut(s) 496, 680
SexAI ACCWGGT 1 cut(s) 607
SfaNI GCATC 4 cut(s) 700, 882, 1087, 1541
SfcI CTRYAG 5 cut(s) 669, 786, 840, 987, 1188
SinI GGWCC 3 cut(s) 40, 854, 1473
SmaI CCCGGG 1 cut(s) 1029
SmlI CTYRAG 3 cut(s) 311, 1344, 1368
SmoI CTYRAG 3 cut(s) 311, 1344, 1368
SnaBI TACGTA 1 cut(s) 1280
Sse9I AATT 8 cut(s) 378, 463, 486, 642, 714, 792, 1034, 1309
SsiI CCGC 1 cut(s) 1106
SspMI CTAG 3 cut(s) 113, 261, 1508
SstI GAGCTC 1 cut(s) 496
StyD4I CCNGG 7 cut(s) 54, 607, 856, 1026, 1027, 1081, 1395
StyI CCWWGG 3 cut(s) 103, 538, 1464
TaaI ACNGT 4 cut(s) 851, 871, 1270, 1542
TaiI ACGT 4 cut(s) 127, 272, 1264, 1282
TaqI TCGA 1 cut(s) 328
TasI AATT 8 cut(s) 378, 463, 486, 642, 714, 792, 1034, 1309
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 3 cut(s) 59, 389, 1003
Tru1I TTAA 4 cut(s) 969, 1119, 1494, 1549
Tru9I TTAA 4 cut(s) 969, 1119, 1494, 1549
TscAI CASTG 5 cut(s) 211, 343, 379, 556, 856
TseFI GTSAC 2 cut(s) 334, 1270
TseI GCWGC 9 cut(s) 144, 194, 422, 563, 647, 698, 905, 1526, 1529
Tsp45I GTSAC 2 cut(s) 334, 1270
TspDTI ATGAA 8 cut(s) 48, 75, 228, 423, 446, 543, 1016, 1535
TspGWI ACGGA 4 cut(s) 241, 612, 1378, 1494
TspMI CCCGGG 1 cut(s) 1027
TspRI CASTG 5 cut(s) 211, 343, 379, 556, 856
Tth111I GACNNNGTC 1 cut(s) 289
VpaK11BI GGWCC 3 cut(s) 40, 854, 1473
XapI RAATTY 3 cut(s) 463, 1034, 1309
XceI RCATGY 1 cut(s) 94
XmaI CCCGGG 1 cut(s) 1027
XmiI GTMKAC 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 3 cut(s) 113, 261, 1508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.