subtilase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1241 |
| Acc65I | GGTACC | 1 cut(s) | 1898 |
| AccB1I | GGYRCC | 1 cut(s) | 1898 |
| AccI | GTMKAC | 1 cut(s) | 1659 |
| AccII | CGCG | 2 cut(s) | 1344, 1452 |
| AccIII | TCCGGA | 2 cut(s) | 309, 1484 |
| AclWI | GGATC | 2 cut(s) | 74, 1951 |
| AcoI | YGGCCR | 3 cut(s) | 440, 867, 1651 |
| AcyI | GRCGYC | 1 cut(s) | 341 |
| AfaI | GTAC | 7 cut(s) | 598, 747, 1076, 1472, 1479, 1529, 1900 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 337, 726, 978, 1275 |
| AflII | CTTAAG | 2 cut(s) | 1132, 1373 |
| AflIII | ACRYGT | 2 cut(s) | 1462, 1507 |
| AgsI | TTSAA | 6 cut(s) | 173, 235, 1090, 1702, 1876, 1996 |
| AjiI | CACGTC | 1 cut(s) | 1463 |
| AjnI | CCWGG | 2 cut(s) | 1393, 1681 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2038, 2070 |
| AleI | CACNNNNGTG | 1 cut(s) | 926 |
| Alw21I | GWGCWC | 1 cut(s) | 891 |
| Alw26I | GTCTC | 8 cut(s) | 29, 41, 67, 132, 549, 802, 1249, 1560 |
| Alw44I | GTGCAC | 1 cut(s) | 887 |
| AlwI | GGATC | 2 cut(s) | 74, 1951 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 620, 2021 |
| Ama87I | CYCGRG | 1 cut(s) | 803 |
| Aor13HI | TCCGGA | 2 cut(s) | 309, 1484 |
| AoxI | GGCC | 5 cut(s) | 246, 440, 867, 1202, 1651 |
| ApaLI | GTGCAC | 1 cut(s) | 887 |
| ApeKI | GCWGC | 5 cut(s) | 701, 959, 1412, 1523, 2031 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1869, 1901 |
| AsiSI | GCGATCGC | 1 cut(s) | 771 |
| Asp718I | GGTACC | 1 cut(s) | 1898 |
| AspLEI | GCGC | 2 cut(s) | 940, 1452 |
| AspS9I | GGNCC | 7 cut(s) | 247, 476, 580, 908, 1203, 1266, 1346 |
| AsuHPI | GGTGA | 8 cut(s) | 16, 172, 223, 1114, 1413, 1784, 1855, 1879 |
| AsuII | TTCGAA | 1 cut(s) | 1748 |
| AvaI | CYCGRG | 1 cut(s) | 803 |
| AvaII | GGWCC | 5 cut(s) | 476, 580, 908, 1266, 1346 |
| BaeGI | GKGCMC | 1 cut(s) | 891 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1120, 1153, 1882, 1915 |
| BalI | TGGCCA | 3 cut(s) | 442, 869, 1653 |
| BanI | GGYRCC | 1 cut(s) | 1898 |
| BanII | GRGCYC | 1 cut(s) | 119 |
| Bbv12I | GWGCWC | 1 cut(s) | 891 |
| BbvI | GCAGC | 5 cut(s) | 713, 971, 1399, 1510, 2043 |
| BccI | CCATC | 4 cut(s) | 1444, 1586, 2075, 2117 |
| BceAI | ACGGC | 4 cut(s) | 354, 380, 925, 1763 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2020, 2054 |
| BciT130I | CCWGG | 2 cut(s) | 1395, 1683 |
| BciVI | GTATCC | 2 cut(s) | 631, 1074 |
| BcoDI | GTCTC | 8 cut(s) | 29, 41, 67, 132, 549, 802, 1249, 1560 |
| BfaI | CTAG | 3 cut(s) | 576, 693, 1971 |
| BfmI | CTRYAG | 1 cut(s) | 1167 |
| BfoI | RGCGCY | 1 cut(s) | 941 |
| BfrI | CTTAAG | 2 cut(s) | 1132, 1373 |
| BfuI | GTATCC | 2 cut(s) | 631, 1074 |
| BglII | AGATCT | 1 cut(s) | 1113 |
| BisI | GCNGC | 8 cut(s) | 618, 702, 960, 1413, 1524, 1671, 1732, 2032 |
| BlsI | GCNGC | 8 cut(s) | 619, 703, 961, 1414, 1525, 1672, 1733, 2033 |
| BmcAI | AGTACT | 1 cut(s) | 1472 |
| Bme1390I | CCNGG | 2 cut(s) | 1395, 1683 |
| Bme18I | GGWCC | 5 cut(s) | 476, 580, 908, 1266, 1346 |
| BmeT110I | CYCGRG | 1 cut(s) | 803 |
| BmgBI | CACGTC | 1 cut(s) | 1463 |
| BmgT120I | GGNCC | 7 cut(s) | 247, 476, 580, 908, 1203, 1266, 1346 |
| BmiI | GGNNCC | 4 cut(s) | 581, 1205, 1268, 1900 |
| BmrFI | CCNGG | 2 cut(s) | 1395, 1683 |
| BmrI | ACTGGG | 1 cut(s) | 1626 |
| BmsI | GCATC | 3 cut(s) | 754, 1196, 1765 |
| BmuI | ACTGGG | 1 cut(s) | 1626 |
| Bpu14I | TTCGAA | 1 cut(s) | 1748 |
| BpuEI | CTTGAG | 2 cut(s) | 69, 1091 |
| BsaHI | GRCGYC | 1 cut(s) | 341 |
| BsaI | GGTCTC | 1 cut(s) | 1560 |
| BsaJI | CCNNGG | 4 cut(s) | 586, 941, 1342, 1894 |
| BsaWI | WCCGGW | 3 cut(s) | 182, 309, 1484 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1241, 1271, 1703, 1733 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 337, 726, 978, 1275 |
| Bse118I | RCCGGY | 1 cut(s) | 896 |
| Bse1I | ACTGG | 2 cut(s) | 1615, 1621 |
| Bse3DI | GCAATG | 1 cut(s) | 667 |
| BseAI | TCCGGA | 2 cut(s) | 309, 1484 |
| BseBI | CCWGG | 2 cut(s) | 1395, 1683 |
| BseDI | CCNNGG | 4 cut(s) | 586, 941, 1342, 1894 |
| BseGI | GGATG | 2 cut(s) | 951, 1543 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 337, 726, 978, 1275 |
| BseMI | GCAATG | 1 cut(s) | 667 |
| BseMII | CTCAG | 5 cut(s) | 78, 249, 906, 1284, 1431 |
| BseNI | ACTGG | 2 cut(s) | 1615, 1621 |
| BseRI | GAGGAG | 3 cut(s) | 17, 241, 471 |
| BseSI | GKGCMC | 1 cut(s) | 891 |
| BseXI | GCAGC | 5 cut(s) | 713, 971, 1399, 1510, 2043 |
| BsgI | GTGCAG | 1 cut(s) | 700 |
| Bsh1236I | CGCG | 2 cut(s) | 1344, 1452 |
| Bsh1285I | CGRYCG | 2 cut(s) | 771, 1632 |
| BshFI | GGCC | 5 cut(s) | 248, 442, 869, 1204, 1653 |
| BshNI | GGYRCC | 1 cut(s) | 1898 |
| BsiEI | CGRYCG | 2 cut(s) | 771, 1632 |
| BsiHKAI | GWGCWC | 1 cut(s) | 891 |
| BsiHKCI | CYCGRG | 1 cut(s) | 803 |
| BsiSI | CCGG | 4 cut(s) | 183, 310, 897, 1485 |
| BslFI | GGGAC | 3 cut(s) | 362, 593, 1252 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 337, 726, 978, 1275 |
| BsmAI | GTCTC | 8 cut(s) | 29, 41, 67, 132, 549, 802, 1249, 1560 |
| BsmBI | CGTCTC | 3 cut(s) | 132, 802, 1249 |
| BsmFI | GGGAC | 3 cut(s) | 362, 593, 1252 |
| BsmI | GAATGC | 2 cut(s) | 1042, 1609 |
| BsnI | GGCC | 5 cut(s) | 248, 442, 869, 1204, 1653 |
| Bso31I | GGTCTC | 1 cut(s) | 1560 |
| BsoBI | CYCGRG | 1 cut(s) | 803 |
| Bsp119I | TTCGAA | 1 cut(s) | 1748 |
| Bsp1286I | GDGCHC | 2 cut(s) | 119, 891 |
| Bsp13I | TCCGGA | 2 cut(s) | 309, 1484 |
| Bsp143I | GATC | 6 cut(s) | 79, 543, 768, 1045, 1113, 1943 |
| BspANI | GGCC | 5 cut(s) | 248, 442, 869, 1204, 1653 |
| BspCNI | CTCAG | 5 cut(s) | 77, 250, 905, 1285, 1432 |
| BspEI | TCCGGA | 2 cut(s) | 309, 1484 |
| BspFNI | CGCG | 2 cut(s) | 1344, 1452 |
| BspHI | TCATGA | 1 cut(s) | 1581 |
| BspLI | GGNNCC | 4 cut(s) | 581, 1205, 1268, 1900 |
| BspPI | GGATC | 2 cut(s) | 74, 1951 |
| BspT104I | TTCGAA | 1 cut(s) | 1748 |
| BspT107I | GGYRCC | 1 cut(s) | 1898 |
| BspTI | CTTAAG | 2 cut(s) | 1132, 1373 |
| BspTNI | GGTCTC | 1 cut(s) | 1560 |
| BsrDI | GCAATG | 1 cut(s) | 667 |
| BsrFI | RCCGGY | 1 cut(s) | 896 |
| BsrI | ACTGG | 2 cut(s) | 1615, 1621 |
| BssAI | RCCGGY | 1 cut(s) | 896 |
| BssECI | CCNNGG | 4 cut(s) | 586, 941, 1342, 1894 |
| BssMI | GATC | 6 cut(s) | 79, 543, 768, 1045, 1113, 1943 |
| BssNI | GRCGYC | 1 cut(s) | 341 |
| BssT1I | CCWWGG | 2 cut(s) | 586, 1894 |
| Bst2UI | CCWGG | 2 cut(s) | 1395, 1683 |
| Bst6I | CTCTTC | 1 cut(s) | 1342 |
| BstACI | GRCGYC | 1 cut(s) | 341 |
| BstAFI | CTTAAG | 2 cut(s) | 1132, 1373 |
| BstBI | TTCGAA | 1 cut(s) | 1748 |
| BstC8I | GCNNGC | 1 cut(s) | 1651 |
| BstDEI | CTNAG | 7 cut(s) | 64, 258, 486, 892, 1293, 1440, 1947 |
| BstDSI | CCRYGG | 2 cut(s) | 941, 1342 |
| BstEII | GGTNACC | 1 cut(s) | 178 |
| BstF5I | GGATG | 2 cut(s) | 951, 1543 |
| BstFNI | CGCG | 2 cut(s) | 1344, 1452 |
| BstH2I | RGCGCY | 1 cut(s) | 941 |
| BstHHI | GCGC | 2 cut(s) | 940, 1452 |
| BstKTI | GATC | 6 cut(s) | 82, 546, 771, 1048, 1116, 1946 |
| BstMAI | GTCTC | 8 cut(s) | 29, 41, 67, 132, 549, 802, 1249, 1560 |
| BstMBI | GATC | 6 cut(s) | 79, 543, 768, 1045, 1113, 1943 |
| BstMCI | CGRYCG | 2 cut(s) | 771, 1632 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 626, 851, 860, 902, 1646 |
| BstNI | CCWGG | 2 cut(s) | 1395, 1683 |
| BstPI | GGTNACC | 1 cut(s) | 178 |
| BstSCI | CCNGG | 2 cut(s) | 1393, 1681 |
| BstSFI | CTRYAG | 1 cut(s) | 1167 |
| BstSLI | GKGCMC | 1 cut(s) | 891 |
| BstUI | CGCG | 2 cut(s) | 1344, 1452 |
| BstV1I | GCAGC | 5 cut(s) | 713, 971, 1399, 1510, 2043 |
| BstX2I | RGATCY | 2 cut(s) | 1113, 1943 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 593 |
| BstYI | RGATCY | 2 cut(s) | 1113, 1943 |
| BsuI | GTATCC | 2 cut(s) | 631, 1074 |
| BsuRI | GGCC | 5 cut(s) | 248, 442, 869, 1204, 1653 |
| BtgI | CCRYGG | 2 cut(s) | 941, 1342 |
| BtgZI | GCGATG | 1 cut(s) | 750 |
| BtrI | CACGTC | 1 cut(s) | 1463 |
| BtsCI | GGATG | 2 cut(s) | 951, 1543 |
| BtsI | GCAGTG | 1 cut(s) | 1795 |
| BtsIMutI | CAGTG | 6 cut(s) | 255, 436, 882, 1038, 1795, 2022 |
| Cac8I | GCNNGC | 1 cut(s) | 1651 |
| CaiI | CAGNNNCTG | 2 cut(s) | 620, 2021 |
| CciI | TCATGA | 1 cut(s) | 1581 |
| CfoI | GCGC | 2 cut(s) | 940, 1452 |
| Cfr10I | RCCGGY | 1 cut(s) | 896 |
| Cfr13I | GGNCC | 7 cut(s) | 247, 476, 580, 908, 1203, 1266, 1346 |
| Cfr42I | CCGCGG | 1 cut(s) | 1345 |
| CseI | GACGC | 2 cut(s) | 330, 784 |
| Csp6I | GTAC | 7 cut(s) | 597, 746, 1075, 1471, 1478, 1528, 1899 |
| CviAII | CATG | 7 cut(s) | 33, 355, 593, 1582, 1655, 1735, 1940 |
| CviQI | GTAC | 7 cut(s) | 597, 746, 1075, 1471, 1478, 1528, 1899 |
| DdeI | CTNAG | 7 cut(s) | 64, 258, 486, 892, 1293, 1440, 1947 |
| DpnI | GATC | 6 cut(s) | 81, 545, 770, 1047, 1115, 1945 |
| DpnII | GATC | 6 cut(s) | 79, 543, 768, 1045, 1113, 1943 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1241 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1241 |
| EaeI | YGGCCR | 3 cut(s) | 440, 867, 1651 |
| Eam1104I | CTCTTC | 1 cut(s) | 1342 |
| EarI | CTCTTC | 1 cut(s) | 1342 |
| Eco130I | CCWWGG | 2 cut(s) | 586, 1894 |
| Eco24I | GRGCYC | 1 cut(s) | 119 |
| Eco31I | GGTCTC | 1 cut(s) | 1560 |
| Eco47I | GGWCC | 5 cut(s) | 476, 580, 908, 1266, 1346 |
| Eco88I | CYCGRG | 1 cut(s) | 803 |
| Eco91I | GGTNACC | 1 cut(s) | 178 |
| EcoO109I | RGGNCCY | 2 cut(s) | 580, 1266 |
| EcoO65I | GGTNACC | 1 cut(s) | 178 |
| EcoRII | CCWGG | 2 cut(s) | 1393, 1681 |
| EcoT14I | CCWWGG | 2 cut(s) | 586, 1894 |
| EcoT22I | ATGCAT | 1 cut(s) | 1189 |
| EcoT38I | GRGCYC | 1 cut(s) | 119 |
| ErhI | CCWWGG | 2 cut(s) | 586, 1894 |
| Esp3I | CGTCTC | 3 cut(s) | 132, 802, 1249 |
| FaeI | CATG | 7 cut(s) | 36, 358, 596, 1585, 1658, 1738, 1943 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 374, 406, 984, 1016, 1916, 1948 |
| FaqI | GGGAC | 3 cut(s) | 362, 593, 1252 |
| FatI | CATG | 7 cut(s) | 32, 354, 592, 1581, 1654, 1734, 1939 |
| FauI | CCCGC | 2 cut(s) | 1278, 1349 |
| FblI | GTMKAC | 1 cut(s) | 1659 |
| Fnu4HI | GCNGC | 8 cut(s) | 618, 702, 960, 1413, 1524, 1671, 1732, 2032 |
| FokI | GGATG | 2 cut(s) | 958, 1530 |
| FriOI | GRGCYC | 1 cut(s) | 119 |
| Fsp4HI | GCNGC | 8 cut(s) | 618, 702, 960, 1413, 1524, 1671, 1732, 2032 |
| FspBI | CTAG | 3 cut(s) | 576, 693, 1971 |
| GlaI | GCGC | 2 cut(s) | 939, 1451 |
| GluI | GCNGC | 8 cut(s) | 618, 702, 960, 1413, 1524, 1671, 1732, 2032 |
| HaeII | RGCGCY | 1 cut(s) | 941 |
| HaeIII | GGCC | 5 cut(s) | 248, 442, 869, 1204, 1653 |
| HapII | CCGG | 4 cut(s) | 183, 310, 897, 1485 |
| HgaI | GACGC | 2 cut(s) | 330, 784 |
| HhaI | GCGC | 2 cut(s) | 940, 1452 |
| Hin1I | GRCGYC | 1 cut(s) | 341 |
| Hin1II | CATG | 7 cut(s) | 36, 358, 596, 1585, 1658, 1738, 1943 |
| Hin6I | GCGC | 2 cut(s) | 938, 1450 |
| HinP1I | GCGC | 2 cut(s) | 938, 1450 |
| HincII | GTYRAC | 3 cut(s) | 784, 951, 1660 |
| HindII | GTYRAC | 3 cut(s) | 784, 951, 1660 |
| HindIII | AAGCTT | 1 cut(s) | 643 |
| HpaI | GTTAAC | 1 cut(s) | 951 |
| HpaII | CCGG | 4 cut(s) | 183, 310, 897, 1485 |
| HphI | GGTGA | 8 cut(s) | 16, 172, 223, 1114, 1413, 1784, 1855, 1879 |
| Hpy166II | GTNNAC | 7 cut(s) | 612, 784, 889, 951, 1433, 1505, 1660 |
| Hpy8I | GTNNAC | 7 cut(s) | 612, 784, 889, 951, 1433, 1505, 1660 |
| Hpy99I | CGWCG | 1 cut(s) | 340 |
| HpyCH4IV | ACGT | 5 cut(s) | 608, 744, 1462, 1509, 2094 |
| HpyCH4V | TGCA | 7 cut(s) | 717, 830, 889, 959, 1187, 1788, 2063 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 626, 851, 860, 902, 1646 |
| HpyF3I | CTNAG | 7 cut(s) | 64, 258, 486, 892, 1293, 1440, 1947 |
| HpySE526I | ACGT | 5 cut(s) | 608, 744, 1462, 1509, 2094 |
| Hsp92I | GRCGYC | 1 cut(s) | 341 |
| Hsp92II | CATG | 7 cut(s) | 36, 358, 596, 1585, 1658, 1738, 1943 |
| HspAI | GCGC | 2 cut(s) | 938, 1450 |
| Kpn2I | TCCGGA | 2 cut(s) | 309, 1484 |
| KpnI | GGTACC | 1 cut(s) | 1902 |
| KspAI | GTTAAC | 1 cut(s) | 951 |
| KspI | CCGCGG | 1 cut(s) | 1345 |
| Kzo9I | GATC | 6 cut(s) | 79, 543, 768, 1045, 1113, 1943 |
| LmnI | GCTCC | 3 cut(s) | 548, 1646, 1862 |
| Lsp1109I | GCAGC | 5 cut(s) | 713, 971, 1399, 1510, 2043 |
| LweI | GCATC | 3 cut(s) | 754, 1196, 1765 |
| MaeI | CTAG | 3 cut(s) | 576, 693, 1971 |
| MaeII | ACGT | 5 cut(s) | 608, 744, 1462, 1509, 2094 |
| MaeIII | GTNAC | 9 cut(s) | 178, 218, 530, 899, 1510, 1867, 1885, 2017, 2126 |
| MalI | GATC | 6 cut(s) | 81, 545, 770, 1047, 1115, 1945 |
| MboI | GATC | 6 cut(s) | 79, 543, 768, 1045, 1113, 1943 |
| MboII | GAAGA | 2 cut(s) | 161, 1329 |
| MfeI | CAATTG | 1 cut(s) | 840 |
| MflI | RGATCY | 2 cut(s) | 1113, 1943 |
| MhlI | GDGCHC | 2 cut(s) | 119, 891 |
| MlsI | TGGCCA | 3 cut(s) | 442, 869, 1653 |
| MluCI | AATT | 3 cut(s) | 69, 840, 1764 |
| MluNI | TGGCCA | 3 cut(s) | 442, 869, 1653 |
| MlyI | GAGTC | 3 cut(s) | 269, 705, 1229 |
| MmeI | TCCRAC | 1 cut(s) | 941 |
| Mox20I | TGGCCA | 3 cut(s) | 442, 869, 1653 |
| Mph1103I | ATGCAT | 1 cut(s) | 1189 |
| MroI | TCCGGA | 2 cut(s) | 309, 1484 |
| MscI | TGGCCA | 3 cut(s) | 442, 869, 1653 |
| MseI | TTAA | 9 cut(s) | 950, 986, 1133, 1254, 1287, 1305, 1374, 1758, 1934 |
| MslI | CAYNNNNRTG | 4 cut(s) | 146, 359, 926, 2111 |
| Msp20I | TGGCCA | 3 cut(s) | 442, 869, 1653 |
| MspA1I | CMGCKG | 2 cut(s) | 617, 1344 |
| MspCI | CTTAAG | 2 cut(s) | 1132, 1373 |
| MspI | CCGG | 4 cut(s) | 183, 310, 897, 1485 |
| MspR9I | CCNGG | 2 cut(s) | 1395, 1683 |
| MunI | CAATTG | 1 cut(s) | 840 |
| Mva1269I | GAATGC | 2 cut(s) | 1042, 1609 |
| MvaI | CCWGG | 2 cut(s) | 1395, 1683 |
| MvnI | CGCG | 2 cut(s) | 1344, 1452 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 626, 851, 860, 902, 1646 |
| NdeII | GATC | 6 cut(s) | 79, 543, 768, 1045, 1113, 1943 |
| NlaIII | CATG | 7 cut(s) | 36, 358, 596, 1585, 1658, 1738, 1943 |
| NlaIV | GGNNCC | 4 cut(s) | 581, 1205, 1268, 1900 |
| NmeAIII | GCCGAG | 2 cut(s) | 224, 1180 |
| NmuCI | GTSAC | 4 cut(s) | 178, 1867, 1885, 2017 |
| NsiI | ATGCAT | 1 cut(s) | 1189 |
| NspV | TTCGAA | 1 cut(s) | 1748 |
| OliI | CACNNNNGTG | 1 cut(s) | 926 |
| PagI | TCATGA | 1 cut(s) | 1581 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 423, 1515 |
| PctI | GAATGC | 2 cut(s) | 1042, 1609 |
| PfeI | GAWTC | 9 cut(s) | 154, 420, 446, 877, 913, 1094, 1405, 1625, 1847 |
| PflFI | GACNNNGTC | 1 cut(s) | 340 |
| PfoI | TCCNGGA | 1 cut(s) | 1681 |
| PkrI | GCNGC | 8 cut(s) | 619, 703, 961, 1414, 1525, 1672, 1733, 2033 |
| Ple19I | CGATCG | 1 cut(s) | 771 |
| PleI | GAGTC | 3 cut(s) | 268, 704, 1229 |
| PpsI | GAGTC | 3 cut(s) | 268, 704, 1229 |
| PpuMI | RGGWCCY | 2 cut(s) | 580, 1266 |
| Psp5II | RGGWCCY | 2 cut(s) | 580, 1266 |
| Psp6I | CCWGG | 2 cut(s) | 1393, 1681 |
| PspEI | GGTNACC | 1 cut(s) | 178 |
| PspGI | CCWGG | 2 cut(s) | 1393, 1681 |
| PspN4I | GGNNCC | 4 cut(s) | 581, 1205, 1268, 1900 |
| PspPI | GGNCC | 7 cut(s) | 247, 476, 580, 908, 1203, 1266, 1346 |
| PspPPI | RGGWCCY | 2 cut(s) | 580, 1266 |
| PstNI | CAGNNNCTG | 2 cut(s) | 620, 2021 |
| PsuI | RGATCY | 2 cut(s) | 1113, 1943 |
| PsyI | GACNNNGTC | 1 cut(s) | 340 |
| PvuI | CGATCG | 1 cut(s) | 771 |
| RgaI | GCGATCGC | 1 cut(s) | 771 |
| RsaI | GTAC | 7 cut(s) | 598, 747, 1076, 1472, 1479, 1529, 1900 |
| RsaNI | GTAC | 7 cut(s) | 597, 746, 1075, 1471, 1478, 1528, 1899 |
| RseI | CAYNNNNRTG | 4 cut(s) | 146, 359, 926, 2111 |
| SacII | CCGCGG | 1 cut(s) | 1345 |
| SalI | GTCGAC | 1 cut(s) | 1658 |
| SaqAI | TTAA | 9 cut(s) | 950, 986, 1133, 1254, 1287, 1305, 1374, 1758, 1934 |
| SatI | GCNGC | 8 cut(s) | 618, 702, 960, 1413, 1524, 1671, 1732, 2032 |
| Sau3AI | GATC | 6 cut(s) | 79, 543, 768, 1045, 1113, 1943 |
| Sau96I | GGNCC | 7 cut(s) | 247, 476, 580, 908, 1203, 1266, 1346 |
| ScaI | AGTACT | 1 cut(s) | 1472 |
| SchI | GAGTC | 3 cut(s) | 269, 705, 1229 |
| ScrFI | CCNGG | 2 cut(s) | 1395, 1683 |
| SduI | GDGCHC | 2 cut(s) | 119, 891 |
| SfaAI | GCGATCGC | 1 cut(s) | 771 |
| SfaNI | GCATC | 3 cut(s) | 754, 1196, 1765 |
| SfcI | CTRYAG | 1 cut(s) | 1167 |
| Sfr303I | CCGCGG | 1 cut(s) | 1345 |
| SfuI | TTCGAA | 1 cut(s) | 1748 |
| SgfI | GCGATCGC | 1 cut(s) | 771 |
| SgrBI | CCGCGG | 1 cut(s) | 1345 |
| SinI | GGWCC | 5 cut(s) | 476, 580, 908, 1266, 1346 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 146, 359, 926, 2111 |
| SmlI | CTYRAG | 4 cut(s) | 84, 1070, 1132, 1373 |
| SmoI | CTYRAG | 4 cut(s) | 84, 1070, 1132, 1373 |
| Sse9I | AATT | 3 cut(s) | 69, 840, 1764 |
| SspI | AATATT | 1 cut(s) | 166 |
| SspMI | CTAG | 3 cut(s) | 576, 693, 1971 |
| StyD4I | CCNGG | 2 cut(s) | 1393, 1681 |
| StyI | CCWWGG | 2 cut(s) | 586, 1894 |
| TaiI | ACGT | 5 cut(s) | 611, 747, 1465, 1512, 2097 |
| TaqI | TCGA | 7 cut(s) | 152, 426, 1628, 1659, 1748, 1826, 2040 |
| TaqII | GACCGA | 1 cut(s) | 1646 |
| TasI | AATT | 3 cut(s) | 69, 840, 1764 |
| TatI | WGTACW | 2 cut(s) | 1470, 1477 |
| TauI | GCSGC | 3 cut(s) | 620, 1673, 1734 |
| TfiI | GAWTC | 9 cut(s) | 154, 420, 446, 877, 913, 1094, 1405, 1625, 1847 |
| Tru1I | TTAA | 9 cut(s) | 950, 986, 1133, 1254, 1287, 1305, 1374, 1758, 1934 |
| Tru9I | TTAA | 9 cut(s) | 950, 986, 1133, 1254, 1287, 1305, 1374, 1758, 1934 |
| TscAI | CASTG | 6 cut(s) | 262, 436, 889, 1038, 1795, 2022 |
| TseFI | GTSAC | 4 cut(s) | 178, 1867, 1885, 2017 |
| TseI | GCWGC | 5 cut(s) | 701, 959, 1412, 1523, 2031 |
| Tsp45I | GTSAC | 4 cut(s) | 178, 1867, 1885, 2017 |
| TspDTI | ATGAA | 9 cut(s) | 343, 377, 412, 1146, 1150, 1532, 1616, 1751, 2000 |
| TspGWI | ACGGA | 3 cut(s) | 114, 1092, 1552 |
| TspRI | CASTG | 6 cut(s) | 262, 436, 889, 1038, 1795, 2022 |
| Tth111I | GACNNNGTC | 1 cut(s) | 340 |
| Vha464I | CTTAAG | 2 cut(s) | 1132, 1373 |
| VneI | GTGCAC | 1 cut(s) | 887 |
| VpaK11BI | GGWCC | 5 cut(s) | 476, 580, 908, 1266, 1346 |
| XbaI | TCTAGA | 1 cut(s) | 692 |
| XmiI | GTMKAC | 1 cut(s) | 1659 |
| XspI | CTAG | 3 cut(s) | 576, 693, 1971 |
| ZrmI | AGTACT | 1 cut(s) | 1472 |
| Zsp2I | ATGCAT | 1 cut(s) | 1189 |