Rroxscaffold_4G00326570

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000257
Physical Location & Seq
Forward (+)
58748435 .. 58750639
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326570.1

Sequence Viewer

Length: 2205 bp
ATGGCTAATCATGGAGCTATCCTATTTTCATATGCTTTCCCCACTCTTATACTAACCTTGAGCTTTTTCCTGTGCAAAGCCATTGATGAAAACAGAACGATCCACATTGTGTACCTAGGCTCACTTCCTGAAAATGAGGTGTACTCACCAACGTCTCACCACCTTGGTTTACTCCAAAAAGTTGTCGACAGCAACTCCGCTGCAAATTTCTTGACAAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCCAATCTCACTGACTGGGAAAGGGAAAGGCTTGCTAACATGAAGGAAGTGGTCTCTGTCTTTCCTAGCAGAATTCTCCATCCTCAGACAACAAGATCTTGGGACTTTATTGGTTTCAGTGAGAAAATAAGACGGAATGCCAGTGTTGAGAGTGATACCATTATTGGTGTCATTGATTCTGGAATTTGGCCTGAATCGGAGAGCTTTAAAGATGATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGTGCTTGTGAGGGCGGCCAAAATTTCACTTGCAATAAGAAGCTAATTGGAGCCCGAGTTTACAGTTCATCTTCAGAGTCTGCAAGGGATGAACAAGGCCATGGAACCCACACAGCCTCAACTGCTGCAGGGAACACTGTAAAGGATGTGAGCTTTTATGGACTAGCAGGAGGTACTGCAAGAGGAGGCGTTCCCTCAGCGAGAATTGCTGTGTATAAAGTATGTGGTCAGGATGGGTGCCCTAGTGATGCTATATTGGCTGCTTTTGATGATGCTATTGCGGATGGAGCTGACATCATTACAGTTTCATTAGGAAGCGAGGAAGCATCTTTATTACAGTATGATCCTATTGCTATCGGTGCTTTCCATGCAATGGCAAAGGGGGTACTTACATCAAATTCTGCAGGCAACAGTGGTCCTGATGATTCAACTGTTTCAAGTGTAGCACCATGGATATTTACAGTTGCAGCAAGTAGTACTGATCGTAGGATCATTGACAAGGTTGTTCTCAAAAATGGGACGAACATGGTTGGGACTTCAGTGAACACTTTCAAATTAAATGGGACAAGTTTTCCATTGATCTATGGAAAAGATGCTTCAAGTAATTGCTCCGAGATCGAAGCTGGGAGCTGTTCAGATGGCTGCTTAGATAGTGGTTTAGTTAAGGGAAAAGTTGTGTTATGTGACGTGACTAGCGGAGTTGATGAGGCCTATGTTTCTGGAGCAGTAGGCTTAATTTTGAGAGCTGATTATGATGATGTTTCTGAAATTGTACCCTTTCCTACAACAGCTCTTGCCAACAAAGAGCATAGTCTGATAAAGTTGTACACGAACTCCACCAAAGATCCTCGAGTAAAAATACAAAGAAGTGAAGAAGCAAGGGATACTGCCGCACCTGTTGTTGCTTCATTCTCTTCACGTGGACCAAATTCAATTCTACCAGAAATTATCAAGCCAGATATAAGTGCCCCAGGGGTTACTATTTTGGCTGCCTTTTCACCTATTGCTTCAGTTACAGAGAGTCTTCAAGACACAAGGCGTGTCAAATATAGTATACTATCTGGAACCTCCATGTCTTGTCCCCACGCAGCTGGTGCAGCTGCTTATGTTAAAGCATTCCATCCTGATTGGTCTCCAGCAGCCATCAAATCATCTCTCATGACTACAGCTTCTCCCATGAATGTTACTGACAATAGCAGTGCTGCTGGTGAATTTGCTTATGGATCTGGACATATCAATCCTGTCAAAGCTATAGACCCAGGGCTTGTGTATGACGCTTCTAAGGAAGACTACATAAAGTTGCTATGCTCGGTCTTGGATCATGCCGATGTTAGACTTATATCAGGAGATAACAGCACTTGCTCTACAAGCTCTGACAAAGGATCTTTACAGGATCACAATTACCCTTCACTAGCAGCCGTTGTCACACCAAACAAATCATTTTCGTTGAAATTTAACAGAAAAGTTAAGAATGTTGGCCTTGCAAACTCCACTTACAAGGCCACAATATTCGCCAACTCTACTCAAGTTGACGTCAAAGTGGTGCCTCAAGTTCTTTCCTTCAAGTCCTTGAATGAGGAGAAGACTTTTGATGTGACAGTTGCCGGAAAAGGTTTGCCAGATGAAGTACAATCACATGTGTCTGCATCGCTGGTGTGGTCTGATGGAATTCATAGCGTTAGAAGTCCAATTCTTATCCACAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

734

Amino Acids

78.02

Weight (kDa)

6.12

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 110 1.3e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 575 1.3e-45 Subtilase family
fn3_6 PF17766 630 - 731 5.2e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2031
AccB1I GGYRCC 2 cut(s) 732, 2038
AccB7I CCANNNNNTGG 2 cut(s) 489, 868
AccI GTMKAC 2 cut(s) 186, 1549
AciI CCGC 5 cut(s) 198, 510, 776, 1191, 1386
AclWI GGATC 8 cut(s) 94, 833, 992, 1334, 1726, 1821, 1885, 1896
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 9 cut(s) 205, 321, 432, 517, 892, 1423, 1706, 1946, 2163
AcuI CTGAAG 3 cut(s) 552, 1017, 1488
AcvI CACGTG 1 cut(s) 1415
AcyI GRCGYC 1 cut(s) 2028
AdeI CACNNNGTG 1 cut(s) 109
AfaI GTAC 8 cut(s) 113, 143, 670, 882, 973, 1269, 1322, 2124
AfiI CCNNNNNNNGG 2 cut(s) 489, 868
AflIII ACRYGT 1 cut(s) 2131
AjiI CACGTC 1 cut(s) 1183
AjnI CCWGG 2 cut(s) 1465, 1753
Alw26I GTCTC 3 cut(s) 159, 307, 1632
AlwI GGATC 8 cut(s) 94, 833, 992, 1334, 1726, 1821, 1885, 1896
AlwNI CAGNNNCTG 1 cut(s) 575
Ama87I CYCGRG 2 cut(s) 549, 1344
AoxI GGCC 6 cut(s) 437, 511, 592, 1203, 1972, 1995
ApoI RAATTY 9 cut(s) 205, 321, 432, 517, 892, 1423, 1706, 1946, 2163
Asp700I GAANNNNTTC 1 cut(s) 1043
AspA2I CCTAGG 1 cut(s) 115
AspS9I GGNCC 3 cut(s) 473, 911, 1418
AsuHPI GGTGA 4 cut(s) 138, 149, 1485, 1715
AvaI CYCGRG 2 cut(s) 549, 1344
AvaII GGWCC 3 cut(s) 473, 911, 1418
AvrII CCTAGG 1 cut(s) 115
BaeGI GKGCMC 2 cut(s) 737, 1465
BanI GGYRCC 2 cut(s) 732, 2038
BanII GRGCYC 1 cut(s) 550
BbrPI CACGTG 1 cut(s) 1415
BbsI GAAGAC 3 cut(s) 1511, 1788, 2084
BbvCI CCTCAGC 1 cut(s) 691
BccI CCATC 8 cut(s) 336, 458, 722, 773, 1127, 1623, 1646, 2153
BceAI ACGGC 1 cut(s) 1898
BciT130I CCWGG 2 cut(s) 1467, 1755
BciVI GTATCC 1 cut(s) 1372
BcoDI GTCTC 3 cut(s) 159, 307, 1632
BfaI CTAG 6 cut(s) 116, 315, 659, 738, 1188, 1907
BfmI CTRYAG 4 cut(s) 621, 897, 1659, 1746
BfuI GTATCC 1 cut(s) 1372
BglII AGATCT 1 cut(s) 344
BlnI CCTAGG 1 cut(s) 115
BmcAI AGTACT 1 cut(s) 973
Bme1390I CCNGG 2 cut(s) 1467, 1755
Bme18I GGWCC 3 cut(s) 473, 911, 1418
BmeT110I CYCGRG 2 cut(s) 549, 1344
BmgBI CACGTC 1 cut(s) 1183
BmgT120I GGNCC 3 cut(s) 473, 911, 1418
BmiI GGNNCC 5 cut(s) 547, 601, 734, 1561, 2040
BmrFI CCNGG 2 cut(s) 1467, 1755
BmrI ACTGGG 1 cut(s) 274
BmsI GCATC 5 cut(s) 733, 757, 830, 1078, 2150
BmuI ACTGGG 1 cut(s) 274
BpiI GAAGAC 3 cut(s) 1511, 1788, 2084
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 2 cut(s) 1236, 1614
Bpu10I CCTNAGC 1 cut(s) 691
BpuEI CTTGAG 3 cut(s) 79, 2004, 2028
BsaAI YACGTR 1 cut(s) 1415
BsaHI GRCGYC 1 cut(s) 2028
BsaI GGTCTC 2 cut(s) 307, 1632
BsaJI CCNNGG 8 cut(s) 115, 163, 595, 944, 1465, 1466, 1753, 1754
BsaXI ACNNNNNCTCC 8 cut(s) 179, 209, 537, 567, 1313, 1343, 1651, 1681
Bsc4I CCNNNNNNNGG 2 cut(s) 489, 868
Bse1I ACTGG 2 cut(s) 269, 390
Bse3DI GCAATG 1 cut(s) 873
BseBI CCWGG 2 cut(s) 1467, 1755
BseDI CCNNGG 8 cut(s) 115, 163, 595, 944, 1465, 1466, 1753, 1754
BseGI GGATG 6 cut(s) 328, 589, 646, 733, 784, 1615
BseLI CCNNNNNNNGG 2 cut(s) 489, 868
BseMI GCAATG 1 cut(s) 873
BseMII CTCAG 2 cut(s) 347, 705
BseNI ACTGG 2 cut(s) 269, 390
BseRI GAGGAG 3 cut(s) 468, 693, 2087
BseSI GKGCMC 2 cut(s) 737, 1465
BseYI CCCAGC 1 cut(s) 1118
BsgI GTGCAG 1 cut(s) 1611
BshFI GGCC 6 cut(s) 439, 513, 594, 1205, 1974, 1997
BshNI GGYRCC 2 cut(s) 732, 2038
BsiHKCI CYCGRG 2 cut(s) 549, 1344
BsiSI CCGG 1 cut(s) 2100
BslFI GGGAC 5 cut(s) 365, 1027, 1042, 1072, 1560
BslI CCNNNNNNNGG 2 cut(s) 489, 868
BsmAI GTCTC 3 cut(s) 159, 307, 1632
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 5 cut(s) 365, 1027, 1042, 1072, 1560
BsmI GAATGC 2 cut(s) 391, 1610
BsnI GGCC 6 cut(s) 439, 513, 594, 1205, 1974, 1997
Bso31I GGTCTC 2 cut(s) 307, 1632
BsoBI CYCGRG 2 cut(s) 549, 1344
Bsp1286I GDGCHC 3 cut(s) 550, 737, 1465
Bsp1407I TGTACA 1 cut(s) 1320
Bsp19I CCATGG 2 cut(s) 595, 944
BspACI CCGC 5 cut(s) 198, 510, 776, 1191, 1386
BspANI GGCC 6 cut(s) 439, 513, 594, 1205, 1974, 1997
BspCNI CTCAG 2 cut(s) 346, 704
BspHI TCATGA 1 cut(s) 1653
BspLI GGNNCC 5 cut(s) 547, 601, 734, 1561, 2040
BspMAI CTGCAG 2 cut(s) 625, 901
BspPI GGATC 8 cut(s) 94, 833, 992, 1334, 1726, 1821, 1885, 1896
BspT107I GGYRCC 2 cut(s) 732, 2038
BspTNI GGTCTC 2 cut(s) 307, 1632
BsrDI GCAATG 1 cut(s) 873
BsrGI TGTACA 1 cut(s) 1320
BsrI ACTGG 2 cut(s) 269, 390
BssECI CCNNGG 8 cut(s) 115, 163, 595, 944, 1465, 1466, 1753, 1754
BssNAI GTATAC 1 cut(s) 1550
BssNI GRCGYC 1 cut(s) 2028
BssT1I CCWWGG 4 cut(s) 115, 163, 595, 944
Bst1107I GTATAC 1 cut(s) 1550
Bst2UI CCWGG 2 cut(s) 1467, 1755
Bst4CI ACNGT 8 cut(s) 560, 634, 799, 834, 908, 928, 958, 2095
Bst6I CTCTTC 1 cut(s) 1414
BstACI GRCGYC 1 cut(s) 2028
BstAPI GCANNNNNTGC 1 cut(s) 1589
BstAUI TGTACA 1 cut(s) 1320
BstBAI YACGTR 1 cut(s) 1415
BstC8I GCNNGC 2 cut(s) 282, 901
BstDEI CTNAG 4 cut(s) 333, 691, 1141, 1776
BstDSI CCRYGG 2 cut(s) 595, 944
BstF5I GGATG 6 cut(s) 328, 589, 646, 733, 784, 1615
BstMAI GTCTC 3 cut(s) 159, 307, 1632
BstMWI GCNNNNNNNGC 9 cut(s) 617, 701, 752, 782, 854, 863, 1589, 1592, 1863
BstNI CCWGG 2 cut(s) 1467, 1755
BstNSI RCATGY 1 cut(s) 2135
BstSCI CCNGG 2 cut(s) 1465, 1753
BstSFI CTRYAG 4 cut(s) 621, 897, 1659, 1746
BstSLI GKGCMC 2 cut(s) 737, 1465
BstV2I GAAGAC 3 cut(s) 1511, 1788, 2084
BstX2I RGATCY 4 cut(s) 344, 1339, 1718, 1877
BstXI CCANNNNNNTGG 1 cut(s) 1586
BstYI RGATCY 4 cut(s) 344, 1339, 1718, 1877
BstZ17I GTATAC 1 cut(s) 1550
BsuI GTATCC 1 cut(s) 1372
BsuRI GGCC 6 cut(s) 439, 513, 594, 1205, 1974, 1997
BtgI CCRYGG 2 cut(s) 595, 944
BtgZI GCGATG 1 cut(s) 2127
BtrI CACGTC 1 cut(s) 1183
BtsCI GGATG 6 cut(s) 328, 589, 646, 733, 784, 1615
BtsI GCAGTG 1 cut(s) 1699
BtsIMutI CAGTG 7 cut(s) 258, 373, 397, 630, 913, 1041, 1699
Cac8I GCNNGC 2 cut(s) 282, 901
CaiI CAGNNNCTG 1 cut(s) 575
CciI TCATGA 1 cut(s) 1653
Cfr13I GGNCC 3 cut(s) 473, 911, 1418
CseI GACGC 1 cut(s) 1778
Csp6I GTAC 8 cut(s) 112, 142, 669, 881, 972, 1268, 1321, 2123
CviQI GTAC 8 cut(s) 112, 142, 669, 881, 972, 1268, 1321, 2123
DdeI CTNAG 4 cut(s) 333, 691, 1141, 1776
DraI TTTAAA 1 cut(s) 457
DraIII CACNNNGTG 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 1414
EarI CTCTTC 1 cut(s) 1414
Eco130I CCWWGG 4 cut(s) 115, 163, 595, 944
Eco147I AGGCCT 1 cut(s) 1205
Eco24I GRGCYC 1 cut(s) 550
Eco31I GGTCTC 2 cut(s) 307, 1632
Eco47I GGWCC 3 cut(s) 473, 911, 1418
Eco57I CTGAAG 3 cut(s) 552, 1017, 1488
Eco72I CACGTG 1 cut(s) 1415
Eco88I CYCGRG 2 cut(s) 549, 1344
EcoRI GAATTC 2 cut(s) 321, 2163
EcoRII CCWGG 2 cut(s) 1465, 1753
EcoT14I CCWWGG 4 cut(s) 115, 163, 595, 944
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 4 cut(s) 115, 163, 595, 944
Esp3I CGTCTC 1 cut(s) 159
FaqI GGGAC 5 cut(s) 365, 1027, 1042, 1072, 1560
FauNDI CATATG 1 cut(s) 31
FblI GTMKAC 2 cut(s) 186, 1549
FokI GGATG 6 cut(s) 315, 596, 653, 740, 791, 1602
FriOI GRGCYC 1 cut(s) 550
FspBI CTAG 6 cut(s) 116, 315, 659, 738, 1188, 1907
GsaI CCCAGC 1 cut(s) 1122
GsuI CTGGAG 2 cut(s) 1236, 1614
HaeIII GGCC 6 cut(s) 439, 513, 594, 1205, 1974, 1997
HapII CCGG 1 cut(s) 2100
HgaI GACGC 1 cut(s) 1778
Hin1I GRCGYC 1 cut(s) 2028
HincII GTYRAC 2 cut(s) 187, 2026
HindII GTYRAC 2 cut(s) 187, 2026
HinfI GANTC 5 cut(s) 425, 443, 572, 920, 1516
HpaII CCGG 1 cut(s) 2100
HphI GGTGA 4 cut(s) 138, 149, 1485, 1715
Hpy188I TCNGA 9 cut(s) 336, 448, 571, 1108, 1132, 1261, 1311, 1870, 2158
HpyAV CCTTC 3 cut(s) 286, 1911, 2065
HpyCH4III ACNGT 8 cut(s) 560, 634, 799, 834, 908, 928, 958, 2095
HpyCH4IV ACGT 4 cut(s) 152, 1182, 1414, 2028
HpyF10VI GCNNNNNNNGC 9 cut(s) 617, 701, 752, 782, 854, 863, 1589, 1592, 1863
HpyF3I CTNAG 4 cut(s) 333, 691, 1141, 1776
HpySE526I ACGT 4 cut(s) 152, 1182, 1414, 2028
Hsp92I GRCGYC 1 cut(s) 2028
LmnI GCTCC 6 cut(s) 14, 545, 782, 1109, 1122, 1217
LweI GCATC 5 cut(s) 733, 757, 830, 1078, 2150
MaeI CTAG 6 cut(s) 116, 315, 659, 738, 1188, 1907
MaeII ACGT 4 cut(s) 152, 1182, 1414, 2028
MaeIII GTNAC 8 cut(s) 221, 1178, 1183, 1471, 1507, 1678, 1918, 2089
MboII GAAGA 6 cut(s) 558, 1379, 1401, 1511, 1793, 2089
MflI RGATCY 4 cut(s) 344, 1339, 1718, 1877
MhlI GDGCHC 3 cut(s) 550, 737, 1465
MlyI GAGTC 2 cut(s) 581, 1525
MroXI GAANNNNTTC 1 cut(s) 1043
MseI TTAA 7 cut(s) 456, 1052, 1158, 1229, 1605, 1950, 1962
MslI CAYNNNNRTG 1 cut(s) 1821
MspA1I CMGCKG 3 cut(s) 200, 1586, 1595
MspI CCGG 1 cut(s) 2100
MspR9I CCNGG 2 cut(s) 1467, 1755
Mva1269I GAATGC 2 cut(s) 391, 1610
MvaI CCWGG 2 cut(s) 1467, 1755
MwoI GCNNNNNNNGC 9 cut(s) 617, 701, 752, 782, 854, 863, 1589, 1592, 1863
NcoI CCATGG 2 cut(s) 595, 944
NdeI CATATG 1 cut(s) 31
NlaIV GGNNCC 5 cut(s) 547, 601, 734, 1561, 2040
NmuCI GTSAC 4 cut(s) 1178, 1183, 1918, 2089
NspI RCATGY 1 cut(s) 2135
PaeR7I CTCGAG 1 cut(s) 1344
PagI TCATGA 1 cut(s) 1653
PasI CCCWGGG 2 cut(s) 1466, 1754
PceI AGGCCT 1 cut(s) 1205
PciI ACATGT 1 cut(s) 2131
PctI GAATGC 2 cut(s) 391, 1610
PdmI GAANNNNTTC 1 cut(s) 1043
PfeI GAWTC 3 cut(s) 425, 443, 920
PflMI CCANNNNNTGG 2 cut(s) 489, 868
PleI GAGTC 2 cut(s) 580, 1524
PmaCI CACGTG 1 cut(s) 1415
PmlI CACGTG 1 cut(s) 1415
PpsI GAGTC 2 cut(s) 580, 1524
Ppu21I YACGTR 1 cut(s) 1415
PscI ACATGT 1 cut(s) 2131
Psp6I CCWGG 2 cut(s) 1465, 1753
PspCI CACGTG 1 cut(s) 1415
PspFI CCCAGC 1 cut(s) 1118
PspGI CCWGG 2 cut(s) 1465, 1753
PspN4I GGNNCC 5 cut(s) 547, 601, 734, 1561, 2040
PspPI GGNCC 3 cut(s) 473, 911, 1418
PspXI VCTCGAGB 1 cut(s) 1344
PstI CTGCAG 2 cut(s) 625, 901
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 4 cut(s) 344, 1339, 1718, 1877
PvuII CAGCTG 2 cut(s) 1586, 1595
RsaI GTAC 8 cut(s) 113, 143, 670, 882, 973, 1269, 1322, 2124
RsaNI GTAC 8 cut(s) 112, 142, 669, 881, 972, 1268, 1321, 2123
RseI CAYNNNNRTG 1 cut(s) 1821
SalI GTCGAC 1 cut(s) 185
SaqAI TTAA 7 cut(s) 456, 1052, 1158, 1229, 1605, 1950, 1962
Sau96I GGNCC 3 cut(s) 473, 911, 1418
ScaI AGTACT 1 cut(s) 973
SchI GAGTC 2 cut(s) 581, 1525
ScrFI CCNGG 2 cut(s) 1467, 1755
SduI GDGCHC 3 cut(s) 550, 737, 1465
SfaNI GCATC 5 cut(s) 733, 757, 830, 1078, 2150
SfcI CTRYAG 4 cut(s) 621, 897, 1659, 1746
Sfr274I CTCGAG 1 cut(s) 1344
SinI GGWCC 3 cut(s) 473, 911, 1418
SlaI CTCGAG 1 cut(s) 1344
SmiMI CAYNNNNRTG 1 cut(s) 1821
SmlI CTYRAG 4 cut(s) 58, 1344, 2019, 2043
SmoI CTYRAG 4 cut(s) 58, 1344, 2019, 2043
SseBI AGGCCT 1 cut(s) 1205
SsiI CCGC 5 cut(s) 198, 510, 776, 1191, 1386
SspI AATATT 1 cut(s) 2004
SspMI CTAG 6 cut(s) 116, 315, 659, 738, 1188, 1907
StuI AGGCCT 1 cut(s) 1205
StyD4I CCNGG 2 cut(s) 1465, 1753
StyI CCWWGG 4 cut(s) 115, 163, 595, 944
TaaI ACNGT 8 cut(s) 560, 634, 799, 834, 908, 928, 958, 2095
TaiI ACGT 4 cut(s) 155, 1185, 1417, 2031
TaqI TCGA 3 cut(s) 186, 1113, 1345
TaqII GACCGA 1 cut(s) 1795
TatI WGTACW 4 cut(s) 141, 971, 1320, 2122
TauI GCSGC 2 cut(s) 513, 1388
TfiI GAWTC 3 cut(s) 425, 443, 920
Tru1I TTAA 7 cut(s) 456, 1052, 1158, 1229, 1605, 1950, 1962
Tru9I TTAA 7 cut(s) 456, 1052, 1158, 1229, 1605, 1950, 1962
TscAI CASTG 7 cut(s) 265, 373, 397, 637, 913, 1041, 1699
TseFI GTSAC 4 cut(s) 1178, 1183, 1918, 2089
Tsp45I GTSAC 4 cut(s) 1178, 1183, 1918, 2089
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 7 cut(s) 265, 373, 397, 637, 913, 1041, 1699
Van91I CCANNNNNTGG 2 cut(s) 489, 868
VpaK11BI GGWCC 3 cut(s) 473, 911, 1418
XapI RAATTY 9 cut(s) 205, 321, 432, 517, 892, 1423, 1706, 1946, 2163
XceI RCATGY 1 cut(s) 2135
XhoI CTCGAG 1 cut(s) 1344
XmaJI CCTAGG 1 cut(s) 115
XmiI GTMKAC 2 cut(s) 186, 1549
XmnI GAANNNNTTC 1 cut(s) 1043
XspI CTAG 6 cut(s) 116, 315, 659, 738, 1188, 1907
ZraI GACGTC 1 cut(s) 2029
ZrmI AGTACT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.