Subtilisin-like serine endopeptidase family protein
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1364 |
| AarI | CACCTGC | 1 cut(s) | 1621 |
| Acc36I | ACCTGC | 2 cut(s) | 1204, 1621 |
| AccI | GTMKAC | 2 cut(s) | 21, 1392 |
| AciI | CCGC | 1 cut(s) | 648 |
| AclI | AACGTT | 1 cut(s) | 2082 |
| AcoI | YGGCCR | 1 cut(s) | 1586 |
| AcsI | RAATTY | 5 cut(s) | 233, 342, 427, 737, 1961 |
| AcuI | CTGAAG | 2 cut(s) | 927, 1125 |
| AcvI | CACGTG | 1 cut(s) | 1334 |
| AdeI | CACNNNGTG | 1 cut(s) | 658 |
| AfaI | GTAC | 4 cut(s) | 50, 577, 1463, 2107 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1032, 1819 |
| AflIII | ACRYGT | 1 cut(s) | 1639 |
| AgsI | TTSAA | 7 cut(s) | 148, 238, 368, 522, 1818, 1975, 1984 |
| AhlI | ACTAGT | 1 cut(s) | 931 |
| AjiI | CACGTC | 2 cut(s) | 1007, 1939 |
| AjnI | CCWGG | 3 cut(s) | 93, 174, 1384 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1303, 1335 |
| Alw21I | GWGCWC | 3 cut(s) | 230, 460, 1138 |
| Alw26I | GTCTC | 5 cut(s) | 66, 217, 1192, 1551, 1735 |
| Alw44I | GTGCAC | 1 cut(s) | 1134 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1781 |
| Ama87I | CYCGRG | 1 cut(s) | 1263 |
| AoxI | GGCC | 3 cut(s) | 347, 1586, 1888 |
| ApaLI | GTGCAC | 1 cut(s) | 1134 |
| ApoI | RAATTY | 5 cut(s) | 233, 342, 427, 737, 1961 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 362, 394 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 103, 953 |
| AspLEI | GCGC | 1 cut(s) | 1384 |
| AspS9I | GGNCC | 2 cut(s) | 821, 1337 |
| AsuC2I | CCSGG | 2 cut(s) | 1301, 1668 |
| AsuHPI | GGTGA | 4 cut(s) | 45, 56, 1181, 1785 |
| AvaI | CYCGRG | 1 cut(s) | 1263 |
| AvaII | GGWCC | 2 cut(s) | 821, 1337 |
| BaeGI | GKGCMC | 1 cut(s) | 1138 |
| BalI | TGGCCA | 1 cut(s) | 1588 |
| BamHI | GGATCC | 1 cut(s) | 748 |
| BanII | GRGCYC | 2 cut(s) | 460, 1439 |
| BbrPI | CACGTG | 1 cut(s) | 1334 |
| Bbv12I | GWGCWC | 3 cut(s) | 230, 460, 1138 |
| BccI | CCATC | 8 cut(s) | 64, 224, 683, 722, 1542, 1558, 1925, 2063 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 916, 950 |
| BciT130I | CCWGG | 3 cut(s) | 95, 176, 1386 |
| BciVI | GTATCC | 1 cut(s) | 783 |
| BclI | TGATCA | 1 cut(s) | 123 |
| BcnI | CCSGG | 2 cut(s) | 1301, 1668 |
| BcoDI | GTCTC | 5 cut(s) | 66, 217, 1192, 1551, 1735 |
| BcuI | ACTAGT | 1 cut(s) | 931 |
| BfaI | CTAG | 4 cut(s) | 566, 932, 1139, 1254 |
| BfmI | CTRYAG | 5 cut(s) | 476, 643, 1659, 1773, 1824 |
| BfuAI | ACCTGC | 2 cut(s) | 1204, 1621 |
| BfuI | GTATCC | 1 cut(s) | 783 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1776 |
| BglII | AGATCT | 2 cut(s) | 254, 724 |
| Bme1390I | CCNGG | 5 cut(s) | 95, 176, 1301, 1386, 1668 |
| Bme18I | GGWCC | 2 cut(s) | 821, 1337 |
| BmeT110I | CYCGRG | 1 cut(s) | 1263 |
| BmgBI | CACGTC | 2 cut(s) | 1007, 1939 |
| BmgT120I | GGNCC | 2 cut(s) | 821, 1337 |
| BmiI | GGNNCC | 3 cut(s) | 390, 750, 1480 |
| BmrFI | CCNGG | 5 cut(s) | 95, 176, 1301, 1386, 1668 |
| BmsI | GCATC | 4 cut(s) | 667, 988, 1054, 1084 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 93, 125 |
| BpuMI | CCSGG | 2 cut(s) | 1301, 1668 |
| BsaAI | YACGTR | 1 cut(s) | 1334 |
| BsaI | GGTCTC | 1 cut(s) | 1551 |
| BsaJI | CCNNGG | 5 cut(s) | 70, 94, 777, 854, 1667 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1232, 1262 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1032, 1819 |
| Bse118I | RCCGGY | 1 cut(s) | 827 |
| Bse1I | ACTGG | 3 cut(s) | 1171, 2061, 2093 |
| Bse3DI | GCAATG | 3 cut(s) | 421, 681, 820 |
| BseBI | CCWGG | 3 cut(s) | 95, 176, 1386 |
| BseDI | CCNNGG | 5 cut(s) | 70, 94, 777, 854, 1667 |
| BseGI | GGATG | 1 cut(s) | 1534 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1032, 1819 |
| BseMI | GCAATG | 3 cut(s) | 421, 681, 820 |
| BseMII | CTCAG | 1 cut(s) | 1608 |
| BseNI | ACTGG | 3 cut(s) | 1171, 2061, 2093 |
| BseRI | GAGGAG | 3 cut(s) | 600, 603, 2003 |
| BseSI | GKGCMC | 1 cut(s) | 1138 |
| BseYI | CCCAGC | 1 cut(s) | 1031 |
| BsgI | GTGCAG | 3 cut(s) | 563, 867, 1290 |
| BshFI | GGCC | 3 cut(s) | 349, 1588, 1890 |
| BsiHKAI | GWGCWC | 3 cut(s) | 230, 460, 1138 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1263 |
| BsiSI | CCGG | 3 cut(s) | 828, 1301, 1667 |
| BslFI | GGGAC | 1 cut(s) | 952 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1032, 1819 |
| BsmAI | GTCTC | 5 cut(s) | 66, 217, 1192, 1551, 1735 |
| BsmBI | CGTCTC | 1 cut(s) | 66 |
| BsmFI | GGGAC | 1 cut(s) | 952 |
| BsmI | GAATGC | 2 cut(s) | 1019, 1097 |
| BsnI | GGCC | 3 cut(s) | 349, 1588, 1890 |
| Bso31I | GGTCTC | 1 cut(s) | 1551 |
| BsoBI | CYCGRG | 1 cut(s) | 1263 |
| Bsp1286I | GDGCHC | 4 cut(s) | 230, 460, 1138, 1439 |
| Bsp19I | CCATGG | 1 cut(s) | 777 |
| BspACI | CCGC | 1 cut(s) | 648 |
| BspANI | GGCC | 3 cut(s) | 349, 1588, 1890 |
| BspCNI | CTCAG | 1 cut(s) | 1609 |
| BspHI | TCATGA | 1 cut(s) | 1009 |
| BspLI | GGNNCC | 3 cut(s) | 390, 750, 1480 |
| BspMAI | CTGCAG | 2 cut(s) | 480, 1828 |
| BspMI | ACCTGC | 2 cut(s) | 1204, 1621 |
| BspQI | GCTCTTC | 1 cut(s) | 1988 |
| BspTNI | GGTCTC | 1 cut(s) | 1551 |
| BsrDI | GCAATG | 3 cut(s) | 421, 681, 820 |
| BsrFI | RCCGGY | 1 cut(s) | 827 |
| BsrI | ACTGG | 3 cut(s) | 1171, 2061, 2093 |
| BssAI | RCCGGY | 1 cut(s) | 827 |
| BssECI | CCNNGG | 5 cut(s) | 70, 94, 777, 854, 1667 |
| BssNAI | GTATAC | 1 cut(s) | 22 |
| BssT1I | CCWWGG | 3 cut(s) | 70, 777, 854 |
| Bst1107I | GTATAC | 1 cut(s) | 22 |
| Bst2UI | CCWGG | 3 cut(s) | 95, 176, 1386 |
| Bst4CI | ACNGT | 6 cut(s) | 46, 838, 868, 1061, 1417, 1876 |
| Bst6I | CTCTTC | 3 cut(s) | 94, 1333, 1988 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 608 |
| BstBAI | YACGTR | 1 cut(s) | 1334 |
| BstC8I | GCNNGC | 3 cut(s) | 412, 811, 829 |
| BstDEI | CTNAG | 2 cut(s) | 25, 1617 |
| BstDSI | CCRYGG | 1 cut(s) | 777 |
| BstF5I | GGATG | 1 cut(s) | 1534 |
| BstHHI | GCGC | 1 cut(s) | 1384 |
| BstMAI | GTCTC | 5 cut(s) | 66, 217, 1192, 1551, 1735 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 420, 524, 608, 1127, 1511, 1514, 1622, 1776 |
| BstNI | CCWGG | 3 cut(s) | 95, 176, 1386 |
| BstNSI | RCATGY | 2 cut(s) | 1457, 1643 |
| BstSCI | CCNGG | 5 cut(s) | 93, 174, 1299, 1384, 1666 |
| BstSFI | CTRYAG | 5 cut(s) | 476, 643, 1659, 1773, 1824 |
| BstSLI | GKGCMC | 1 cut(s) | 1138 |
| BstX2I | RGATCY | 7 cut(s) | 254, 724, 748, 1258, 1631, 1663, 1801 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1939 |
| BstYI | RGATCY | 7 cut(s) | 254, 724, 748, 1258, 1631, 1663, 1801 |
| BstZ17I | GTATAC | 1 cut(s) | 22 |
| BsuI | GTATCC | 1 cut(s) | 783 |
| BsuRI | GGCC | 3 cut(s) | 349, 1588, 1890 |
| BtgI | CCRYGG | 1 cut(s) | 777 |
| BtrI | CACGTC | 2 cut(s) | 1007, 1939 |
| BtsCI | GGATG | 1 cut(s) | 1534 |
| BtsI | GCAGTG | 2 cut(s) | 473, 513 |
| BtsIMutI | CAGTG | 5 cut(s) | 473, 513, 951, 1066, 2054 |
| BveI | ACCTGC | 2 cut(s) | 1204, 1621 |
| Cac8I | GCNNGC | 3 cut(s) | 412, 811, 829 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1781 |
| CciI | TCATGA | 1 cut(s) | 1009 |
| CfoI | GCGC | 1 cut(s) | 1384 |
| Cfr10I | RCCGGY | 1 cut(s) | 827 |
| Cfr13I | GGNCC | 2 cut(s) | 821, 1337 |
| Csp6I | GTAC | 4 cut(s) | 49, 576, 1462, 2106 |
| CviQI | GTAC | 4 cut(s) | 49, 576, 1462, 2106 |
| DdeI | CTNAG | 2 cut(s) | 25, 1617 |
| DraI | TTTAAA | 1 cut(s) | 1152 |
| DraIII | CACNNNGTG | 1 cut(s) | 658 |
| EaeI | YGGCCR | 1 cut(s) | 1586 |
| Eam1104I | CTCTTC | 3 cut(s) | 94, 1333, 1988 |
| EarI | CTCTTC | 3 cut(s) | 94, 1333, 1988 |
| Ecl136II | GAGCTC | 1 cut(s) | 458 |
| Eco130I | CCWWGG | 3 cut(s) | 70, 777, 854 |
| Eco24I | GRGCYC | 2 cut(s) | 460, 1439 |
| Eco31I | GGTCTC | 1 cut(s) | 1551 |
| Eco32I | GATATC | 2 cut(s) | 1749, 1945 |
| Eco47I | GGWCC | 2 cut(s) | 821, 1337 |
| Eco53kI | GAGCTC | 1 cut(s) | 458 |
| Eco57I | CTGAAG | 2 cut(s) | 927, 1125 |
| Eco72I | CACGTG | 1 cut(s) | 1334 |
| Eco88I | CYCGRG | 1 cut(s) | 1263 |
| EcoICRI | GAGCTC | 1 cut(s) | 458 |
| EcoO109I | RGGNCCY | 1 cut(s) | 821 |
| EcoRII | CCWGG | 3 cut(s) | 93, 174, 1384 |
| EcoRV | GATATC | 2 cut(s) | 1749, 1945 |
| EcoT14I | CCWWGG | 3 cut(s) | 70, 777, 854 |
| EcoT22I | ATGCAT | 1 cut(s) | 1099 |
| EcoT38I | GRGCYC | 2 cut(s) | 460, 1439 |
| ErhI | CCWWGG | 3 cut(s) | 70, 777, 854 |
| Esp3I | CGTCTC | 1 cut(s) | 66 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1038, 1070 |
| FaqI | GGGAC | 1 cut(s) | 952 |
| FbaI | TGATCA | 1 cut(s) | 123 |
| FblI | GTMKAC | 2 cut(s) | 21, 1392 |
| FokI | GGATG | 1 cut(s) | 1521 |
| FriOI | GRGCYC | 2 cut(s) | 460, 1439 |
| FspBI | CTAG | 4 cut(s) | 566, 932, 1139, 1254 |
| GlaI | GCGC | 1 cut(s) | 1383 |
| GsaI | CCCAGC | 1 cut(s) | 1035 |
| HaeIII | GGCC | 3 cut(s) | 349, 1588, 1890 |
| HapII | CCGG | 3 cut(s) | 828, 1301, 1667 |
| HhaI | GCGC | 1 cut(s) | 1384 |
| Hin6I | GCGC | 1 cut(s) | 1382 |
| HinP1I | GCGC | 1 cut(s) | 1382 |
| HincII | GTYRAC | 1 cut(s) | 697 |
| HindII | GTYRAC | 1 cut(s) | 697 |
| HinfI | GANTC | 6 cut(s) | 273, 353, 635, 1022, 1342, 2089 |
| HpaII | CCGG | 3 cut(s) | 828, 1301, 1667 |
| HphI | GGTGA | 4 cut(s) | 45, 56, 1181, 1785 |
| Hpy166II | GTNNAC | 8 cut(s) | 13, 22, 49, 697, 949, 1136, 1337, 1393 |
| Hpy188III | TCNNGA | 7 cut(s) | 223, 368, 937, 1010, 1476, 1635, 1752 |
| Hpy8I | GTNNAC | 8 cut(s) | 13, 22, 49, 697, 949, 1136, 1337, 1393 |
| Hpy99I | CGWCG | 1 cut(s) | 894 |
| HpyAV | CCTTC | 5 cut(s) | 196, 812, 1333, 1824, 1981 |
| HpyCH4III | ACNGT | 6 cut(s) | 46, 838, 868, 1061, 1417, 1876 |
| HpyCH4IV | ACGT | 4 cut(s) | 1006, 1333, 1938, 2082 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 420, 524, 608, 1127, 1511, 1514, 1622, 1776 |
| HpyF3I | CTNAG | 2 cut(s) | 25, 1617 |
| HpySE526I | ACGT | 4 cut(s) | 1006, 1333, 1938, 2082 |
| HspAI | GCGC | 1 cut(s) | 1382 |
| KroI | GCCGGC | 1 cut(s) | 827 |
| KroNI | GCCGGC | 1 cut(s) | 829 |
| Ksp22I | TGATCA | 1 cut(s) | 123 |
| LguI | GCTCTTC | 1 cut(s) | 1988 |
| LmnI | GCTCC | 2 cut(s) | 394, 455 |
| LweI | GCATC | 4 cut(s) | 667, 988, 1054, 1084 |
| MaeI | CTAG | 4 cut(s) | 566, 932, 1139, 1254 |
| MaeII | ACGT | 4 cut(s) | 1006, 1333, 1938, 2082 |
| MaeIII | GTNAC | 2 cut(s) | 30, 2052 |
| MfeI | CAATTG | 3 cut(s) | 759, 1311, 1540 |
| MflI | RGATCY | 7 cut(s) | 254, 724, 748, 1258, 1631, 1663, 1801 |
| MhlI | GDGCHC | 4 cut(s) | 230, 460, 1138, 1439 |
| MlsI | TGGCCA | 1 cut(s) | 1588 |
| MluNI | TGGCCA | 1 cut(s) | 1588 |
| MlyI | GAGTC | 2 cut(s) | 1016, 2098 |
| MmeI | TCCRAC | 3 cut(s) | 825, 872, 1996 |
| Mox20I | TGGCCA | 1 cut(s) | 1588 |
| Mph1103I | ATGCAT | 1 cut(s) | 1099 |
| MroNI | GCCGGC | 1 cut(s) | 827 |
| MroXI | GAANNNNTTC | 2 cut(s) | 103, 953 |
| MscI | TGGCCA | 1 cut(s) | 1588 |
| MseI | TTAA | 9 cut(s) | 962, 1071, 1151, 1208, 1277, 1524, 1806, 1860, 1878 |
| Msp20I | TGGCCA | 1 cut(s) | 1588 |
| MspI | CCGG | 3 cut(s) | 828, 1301, 1667 |
| MspR9I | CCNGG | 5 cut(s) | 95, 176, 1301, 1386, 1668 |
| MunI | CAATTG | 3 cut(s) | 759, 1311, 1540 |
| Mva1269I | GAATGC | 2 cut(s) | 1019, 1097 |
| MvaI | CCWGG | 3 cut(s) | 95, 176, 1386 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 420, 524, 608, 1127, 1511, 1514, 1622, 1776 |
| NaeI | GCCGGC | 1 cut(s) | 829 |
| NciI | CCSGG | 2 cut(s) | 1301, 1668 |
| NcoI | CCATGG | 1 cut(s) | 777 |
| NgoMIV | GCCGGC | 1 cut(s) | 827 |
| NlaIV | GGNNCC | 3 cut(s) | 390, 750, 1480 |
| NmuCI | GTSAC | 2 cut(s) | 30, 2052 |
| NsiI | ATGCAT | 1 cut(s) | 1099 |
| NspI | RCATGY | 2 cut(s) | 1457, 1643 |
| PaeR7I | CTCGAG | 1 cut(s) | 1263 |
| PagI | TCATGA | 1 cut(s) | 1009 |
| PaqCI | CACCTGC | 1 cut(s) | 1621 |
| PciI | ACATGT | 1 cut(s) | 1639 |
| PciSI | GCTCTTC | 1 cut(s) | 1988 |
| PctI | GAATGC | 2 cut(s) | 1019, 1097 |
| PdiI | GCCGGC | 1 cut(s) | 829 |
| PdmI | GAANNNNTTC | 2 cut(s) | 103, 953 |
| PfeI | GAWTC | 4 cut(s) | 273, 353, 635, 1342 |
| PleI | GAGTC | 2 cut(s) | 1016, 2097 |
| PmaCI | CACGTG | 1 cut(s) | 1334 |
| PmlI | CACGTG | 1 cut(s) | 1334 |
| PpsI | GAGTC | 2 cut(s) | 1016, 2097 |
| Ppu21I | YACGTR | 1 cut(s) | 1334 |
| PpuMI | RGGWCCY | 1 cut(s) | 821 |
| PscI | ACATGT | 1 cut(s) | 1639 |
| PsiI | TTATAA | 1 cut(s) | 1364 |
| Psp124BI | GAGCTC | 1 cut(s) | 460 |
| Psp1406I | AACGTT | 1 cut(s) | 2082 |
| Psp5II | RGGWCCY | 1 cut(s) | 821 |
| Psp6I | CCWGG | 3 cut(s) | 93, 174, 1384 |
| PspCI | CACGTG | 1 cut(s) | 1334 |
| PspFI | CCCAGC | 1 cut(s) | 1031 |
| PspGI | CCWGG | 3 cut(s) | 93, 174, 1384 |
| PspN4I | GGNNCC | 3 cut(s) | 390, 750, 1480 |
| PspPI | GGNCC | 2 cut(s) | 821, 1337 |
| PspPPI | RGGWCCY | 1 cut(s) | 821 |
| PspXI | VCTCGAGB | 1 cut(s) | 1263 |
| PstI | CTGCAG | 2 cut(s) | 480, 1828 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1781 |
| PsuI | RGATCY | 7 cut(s) | 254, 724, 748, 1258, 1631, 1663, 1801 |
| RsaI | GTAC | 4 cut(s) | 50, 577, 1463, 2107 |
| RsaNI | GTAC | 4 cut(s) | 49, 576, 1462, 2106 |
| SacI | GAGCTC | 1 cut(s) | 460 |
| SapI | GCTCTTC | 1 cut(s) | 1988 |
| SaqAI | TTAA | 9 cut(s) | 962, 1071, 1151, 1208, 1277, 1524, 1806, 1860, 1878 |
| Sau96I | GGNCC | 2 cut(s) | 821, 1337 |
| SchI | GAGTC | 2 cut(s) | 1016, 2098 |
| ScrFI | CCNGG | 5 cut(s) | 95, 176, 1301, 1386, 1668 |
| SduI | GDGCHC | 4 cut(s) | 230, 460, 1138, 1439 |
| SfaNI | GCATC | 4 cut(s) | 667, 988, 1054, 1084 |
| SfcI | CTRYAG | 5 cut(s) | 476, 643, 1659, 1773, 1824 |
| Sfr274I | CTCGAG | 1 cut(s) | 1263 |
| SinI | GGWCC | 2 cut(s) | 821, 1337 |
| SlaI | CTCGAG | 1 cut(s) | 1263 |
| SmlI | CTYRAG | 1 cut(s) | 1263 |
| SmoI | CTYRAG | 1 cut(s) | 1263 |
| SpeI | ACTAGT | 1 cut(s) | 931 |
| SsiI | CCGC | 1 cut(s) | 648 |
| SspMI | CTAG | 4 cut(s) | 566, 932, 1139, 1254 |
| SstI | GAGCTC | 1 cut(s) | 460 |
| StyD4I | CCNGG | 5 cut(s) | 93, 174, 1299, 1384, 1666 |
| StyI | CCWWGG | 3 cut(s) | 70, 777, 854 |
| TaaI | ACNGT | 6 cut(s) | 46, 838, 868, 1061, 1417, 1876 |
| TaiI | ACGT | 4 cut(s) | 1009, 1336, 1941, 2085 |
| TaqI | TCGA | 5 cut(s) | 306, 672, 730, 1264, 1412 |
| TaqII | GACCGA | 1 cut(s) | 1354 |
| TatI | WGTACW | 1 cut(s) | 48 |
| TfiI | GAWTC | 4 cut(s) | 273, 353, 635, 1342 |
| Tru1I | TTAA | 9 cut(s) | 962, 1071, 1151, 1208, 1277, 1524, 1806, 1860, 1878 |
| Tru9I | TTAA | 9 cut(s) | 962, 1071, 1151, 1208, 1277, 1524, 1806, 1860, 1878 |
| TscAI | CASTG | 5 cut(s) | 480, 520, 951, 1066, 2061 |
| TseFI | GTSAC | 2 cut(s) | 30, 2052 |
| Tsp45I | GTSAC | 2 cut(s) | 30, 2052 |
| TspGWI | ACGGA | 2 cut(s) | 576, 1176 |
| TspRI | CASTG | 5 cut(s) | 480, 520, 951, 1066, 2061 |
| VneI | GTGCAC | 1 cut(s) | 1134 |
| VpaK11BI | GGWCC | 2 cut(s) | 821, 1337 |
| XapI | RAATTY | 5 cut(s) | 233, 342, 427, 737, 1961 |
| XceI | RCATGY | 2 cut(s) | 1457, 1643 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1472 |
| XhoI | CTCGAG | 1 cut(s) | 1263 |
| XmiI | GTMKAC | 2 cut(s) | 21, 1392 |
| XmnI | GAANNNNTTC | 2 cut(s) | 103, 953 |
| XspI | CTAG | 4 cut(s) | 566, 932, 1139, 1254 |
| Zsp2I | ATGCAT | 1 cut(s) | 1099 |