Rw1G004760

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000257
Physical Location & Seq
Reverse (-)
9110081 .. 9112303
2223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004760.1

Sequence Viewer

Length: 1800 bp
ATGGCAAAGCATAGAGCTCTCCAGGTTTCCTATGTTGTCCCCACTCTTATACTAACCTTGAGCTTCTTCTTCTGCAAAGCCATCGATGAAAACAGAAAGCTCCATATCGTGTACCTGGGGTCACTTCCTGATAGTGAGGTGTACTCACCATTCAACTCCACTTCCAATTTTTTTACAAGAAGTAATTACCAAAGGAGTTTCAATGGATTTGCTGCATACCTAATTGACCGGGAAAGGGAAAGGCTTGCTAGCATGATAGAAGTAGTCTCTGTATTTCCAAGCACAACTTTCCAACTTCAAACGACAAGATCTTGGGACTTTCTCGGTTTCAATGAGAAAATCCAACGAAATGACAGCGTTGAGAGTGATATCATTATTGGTGTCATTGACTCTGGAACTTGGCCTGATTCGGAGTGCTTCAAAGACAATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGTGCTTGTGTAGGTGGCAAAAATTTTACTTGTAATAATAAGCTCATTGGTGCTCGGTTTTACAACTCATCCTCTCAGTCTACAAGGGATGAAACTGGCCATGGAACACACACTGCCTCATCAGCAGCCGGGAATGCTGTAAAGGATGTCAGCTTTTATGGTATAGCCCAAGGCATTGCAAGAGGGGGAGTTCCCTCAGCGAGAGTTGCTGCGTATAGAGCTATCTTGGCTGCTTTTGACGATGCTATTGCCGATGGAGTTGATATCATTACAATTCCAATAGGAAGCCCGGATGTAACTGTACTACTGCAGCATGATCCAATTGCTGTCGGATCTTTCCATGCAATGGAAAAGGGGATACTTACATCAAACTCGGCAGGCAATAATGGTCCTAGAGCTAGTACCGTAACAAGTGTAGCACCATGGTTACTTACAGTTGCAGCAAGTACTACAGATCTGAACTCTTTCCAAATGACTGGGACAAGTTTTCCATTAATATATGGAAAAGATTGTATACAAGACTGCTTAGACCGTGGTTTAGTTAAGGGAAAGGTTGTGTTATATCCACAAGTAAACATACTAAAAAGTGAAGCCGAAAGAGATGCTGCTGCACCTATTGTTGCTTCCTTCTCATCTCCCAGATATAAGGCCCCAGGGGTAGCTATATTGGCTGCTTATTCACCTATTGCTTCAATCACAGACAACCCTGAAGACAAGAGGCATGTAAATTACAGTAGACTTTCCGGAACCTCCATGTCTTGCCCCACGCCACTGGTGCAGCAGCATATGTTTAAGAGTTCCACCCTGATTGCTTCTCCCATGAATTTTACCTACAATGATTATAATGCCGCTCGTGAATTTGCTTGTGGATCTGGACATATCAATCCTGTCAAAGCTATAGACCCGGGGCTTGTATATGAAGCTTCTAAGGAAGACTACATAAATGTTAGACTTATATCAGGATATAACAGCACTTGCTCTACAGGCTCTGACAAAGGATCTTTACATGATCAAAATTACCCATCGCTGGCAGCTGCTGGCACACCAAAGAAACCTTTTTCATTCAAATTTCACAGAAAAGTTCAGAATGTTGGCCTTGCAAACTCCACCTACAAGGCCAAAATATTCGCCGGCAACTCTACTGATGAAGCTGACATCAAAGTGGTGCCTGAAGTCCTTTCCTTCAAGTGCTTGAATGAAGAGAAGACTTTTGATGTGACTGTTGTTGGAAAAGGTTTGTCAGAAGAAGTACAATCACATGTCTCTGCATCGCTGGTGTGGTCTGATGGGATTCATAGCGTTAGAAGTCCAATTCTCATCCACAGAAGTTTTCTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

599

Amino Acids

64.96

Weight (kDa)

7.94

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 61 - 100 1.9e-06 Peptidase inhibitor I9
Peptidase_S8 PF00082 122 - 408 1.7e-16 Subtilase family
fn3_6 PF17766 491 - 593 6e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1302
AccB1I GGYRCC 1 cut(s) 1624
AccB7I CCANNNNNTGG 2 cut(s) 453, 805
AccBSI CCGCTC 1 cut(s) 1310
AccI GTMKAC 3 cut(s) 539, 973, 1195
AccIII TCCGGA 1 cut(s) 1202
AciI CCGC 1 cut(s) 1308
AclWI GGATC 4 cut(s) 770, 799, 1336, 1465
AcoI YGGCCR 1 cut(s) 556
AcsI RAATTY 4 cut(s) 481, 1282, 1316, 1526
AcuI CTGAAG 2 cut(s) 1188, 1650
AfaI GTAC 6 cut(s) 113, 143, 762, 862, 907, 1710
AfiI CCNNNNNNNGG 4 cut(s) 235, 453, 805, 1486
AflIII ACRYGT 1 cut(s) 1717
AgsI TTSAA 9 cut(s) 154, 202, 299, 331, 421, 1152, 1525, 1645, 1654
AjnI CCWGG 3 cut(s) 21, 114, 1111
Alw21I GWGCWC 2 cut(s) 19, 514
Alw26I GTCTC 2 cut(s) 271, 1726
AlwI GGATC 4 cut(s) 770, 799, 1336, 1465
AlwNI CAGNNNCTG 2 cut(s) 1448, 1496
Ama87I CYCGRG 1 cut(s) 1363
Aor13HI TCCGGA 1 cut(s) 1202
AoxI GGCC 5 cut(s) 401, 556, 1107, 1552, 1575
ApoI RAATTY 4 cut(s) 481, 1282, 1316, 1526
ArsI GACNNNNNNTTYG 2 cut(s) 416, 448
AseI ATTAAT 1 cut(s) 953
Asp700I GAANNNNTTC 1 cut(s) 923
AspS9I GGNCC 3 cut(s) 437, 848, 1108
AsuC2I CCSGG 5 cut(s) 230, 589, 749, 1364, 1365
AsuHPI GGTGA 2 cut(s) 138, 1131
AsuNHI GCTAGC 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 1363
AvaII GGWCC 2 cut(s) 437, 848
BalI TGGCCA 1 cut(s) 558
BanI GGYRCC 1 cut(s) 1624
BanII GRGCYC 1 cut(s) 19
BauI CACGAG 1 cut(s) 1311
BbsI GAAGAC 3 cut(s) 1176, 1398, 1670
Bbv12I GWGCWC 2 cut(s) 19, 514
BbvCI CCTCAGC 1 cut(s) 655
BccI CCATC 4 cut(s) 89, 707, 1489, 1739
BcgI CGANNNNNNTGC 6 cut(s) 64, 98, 689, 723, 1043, 1077
BciT130I CCWGG 3 cut(s) 23, 116, 1113
BciVI GTATCC 1 cut(s) 810
BclI TGATCA 1 cut(s) 1468
BcnI CCSGG 5 cut(s) 230, 589, 749, 1364, 1365
BcoDI GTCTC 2 cut(s) 271, 1726
BfaI CTAG 3 cut(s) 249, 852, 858
BfmI CTRYAG 4 cut(s) 767, 909, 1356, 1440
BfuI GTATCC 1 cut(s) 810
BglII AGATCT 2 cut(s) 308, 913
BmcAI AGTACT 1 cut(s) 907
Bme1390I CCNGG 8 cut(s) 23, 116, 230, 589, 749, 1113, 1364, 1365
Bme18I GGWCC 2 cut(s) 437, 848
BmeT110I CYCGRG 1 cut(s) 1363
BmgT120I GGNCC 3 cut(s) 437, 848, 1108
BmiI GGNNCC 3 cut(s) 1110, 1207, 1626
BmrFI CCNGG 8 cut(s) 23, 116, 230, 589, 749, 1113, 1364, 1365
BmrI ACTGGG 1 cut(s) 945
BmsI GCATC 3 cut(s) 691, 1051, 1736
BmtI GCTAGC 1 cut(s) 252
BmuI ACTGGG 1 cut(s) 945
BpiI GAAGAC 3 cut(s) 1176, 1398, 1670
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 1 cut(s) 5
Bpu10I CCTNAGC 1 cut(s) 655
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 5 cut(s) 230, 589, 749, 1364, 1365
Bsa29I ATCGAT 1 cut(s) 84
BsaJI CCNNGG 9 cut(s) 115, 559, 628, 881, 991, 1111, 1112, 1363, 1364
BsaWI WCCGGW 1 cut(s) 1202
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 453, 805, 1486
Bse118I RCCGGY 1 cut(s) 1589
Bse1I ACTGG 3 cut(s) 559, 940, 1236
Bse3DI GCAATG 2 cut(s) 633, 810
BseAI TCCGGA 1 cut(s) 1202
BseBI CCWGG 3 cut(s) 23, 116, 1113
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 9 cut(s) 115, 559, 628, 881, 991, 1111, 1112, 1363, 1364
BseGI GGATG 5 cut(s) 527, 553, 610, 757, 1776
BseLI CCNNNNNNNGG 4 cut(s) 235, 453, 805, 1486
BseMI GCAATG 2 cut(s) 633, 810
BseMII CTCAG 2 cut(s) 548, 669
BseNI ACTGG 3 cut(s) 559, 940, 1236
BseRI GAGGAG 1 cut(s) 432
BsgI GTGCAG 2 cut(s) 1053, 1256
BshFI GGCC 5 cut(s) 403, 558, 1109, 1554, 1577
BshNI GGYRCC 1 cut(s) 1624
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 2 cut(s) 19, 514
BsiHKCI CYCGRG 1 cut(s) 1363
BsiSI CCGG 6 cut(s) 229, 588, 749, 1203, 1364, 1590
BslFI GGGAC 3 cut(s) 23, 329, 952
BslI CCNNNNNNNGG 4 cut(s) 235, 453, 805, 1486
BsmAI GTCTC 2 cut(s) 271, 1726
BsmFI GGGAC 3 cut(s) 23, 329, 952
BsmI GAATGC 1 cut(s) 598
BsnI GGCC 5 cut(s) 403, 558, 1109, 1554, 1577
BsoBI CYCGRG 1 cut(s) 1363
Bsp1286I GDGCHC 2 cut(s) 19, 514
Bsp13I TCCGGA 1 cut(s) 1202
Bsp143I GATC 7 cut(s) 308, 775, 791, 913, 1328, 1457, 1468
Bsp19I CCATGG 2 cut(s) 559, 881
BspACI CCGC 1 cut(s) 1308
BspANI GGCC 5 cut(s) 403, 558, 1109, 1554, 1577
BspCNI CTCAG 2 cut(s) 547, 668
BspDI ATCGAT 1 cut(s) 84
BspEI TCCGGA 1 cut(s) 1202
BspLI GGNNCC 3 cut(s) 1110, 1207, 1626
BspMAI CTGCAG 1 cut(s) 771
BspOI GCTAGC 1 cut(s) 252
BspPI GGATC 4 cut(s) 770, 799, 1336, 1465
BspT107I GGYRCC 1 cut(s) 1624
BsrBI CCGCTC 1 cut(s) 1310
BsrDI GCAATG 2 cut(s) 633, 810
BsrFI RCCGGY 1 cut(s) 1589
BsrI ACTGG 3 cut(s) 559, 940, 1236
BssAI RCCGGY 1 cut(s) 1589
BssECI CCNNGG 9 cut(s) 115, 559, 628, 881, 991, 1111, 1112, 1363, 1364
BssMI GATC 7 cut(s) 308, 775, 791, 913, 1328, 1457, 1468
BssNAI GTATAC 1 cut(s) 974
BssSI CACGAG 1 cut(s) 1311
BssT1I CCWWGG 3 cut(s) 559, 628, 881
Bst1107I GTATAC 1 cut(s) 974
Bst2BI CACGAG 1 cut(s) 1311
Bst2UI CCWGG 3 cut(s) 23, 116, 1113
Bst4CI ACNGT 6 cut(s) 760, 865, 895, 992, 1193, 1681
Bst6I CTCTTC 1 cut(s) 1653
BstC8I GCNNGC 6 cut(s) 246, 250, 838, 1488, 1498, 1591
BstDEI CTNAG 4 cut(s) 534, 655, 985, 1386
BstDSI CCRYGG 3 cut(s) 559, 881, 991
BstF5I GGATG 5 cut(s) 527, 553, 610, 757, 1776
BstKTI GATC 7 cut(s) 311, 778, 794, 916, 1331, 1460, 1471
BstMAI GTCTC 2 cut(s) 271, 1726
BstMBI GATC 7 cut(s) 308, 775, 791, 913, 1328, 1457, 1468
BstMWI GCNNNNNNNGC 8 cut(s) 581, 593, 665, 677, 686, 1127, 1234, 1443
BstNI CCWGG 3 cut(s) 23, 116, 1113
BstNSI RCATGY 2 cut(s) 1184, 1721
BstSCI CCNGG 8 cut(s) 21, 114, 228, 587, 747, 1111, 1362, 1363
BstSFI CTRYAG 4 cut(s) 767, 909, 1356, 1440
BstV2I GAAGAC 3 cut(s) 1176, 1398, 1670
BstX2I RGATCY 5 cut(s) 308, 791, 913, 1328, 1457
BstXI CCANNNNNNTGG 2 cut(s) 935, 1231
BstYI RGATCY 5 cut(s) 308, 791, 913, 1328, 1457
BstZ17I GTATAC 1 cut(s) 974
Bsu15I ATCGAT 1 cut(s) 84
BsuI GTATCC 1 cut(s) 810
BsuRI GGCC 5 cut(s) 403, 558, 1109, 1554, 1577
BsuTUI ATCGAT 1 cut(s) 84
BtgI CCRYGG 3 cut(s) 559, 881, 991
BtgZI GCGATG 2 cut(s) 1467, 1713
BtsCI GGATG 5 cut(s) 527, 553, 610, 757, 1776
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 2 cut(s) 570, 1229
Cac8I GCNNGC 6 cut(s) 246, 250, 838, 1488, 1498, 1591
CaiI CAGNNNCTG 2 cut(s) 1448, 1496
Cfr10I RCCGGY 1 cut(s) 1589
Cfr13I GGNCC 3 cut(s) 437, 848, 1108
Cfr9I CCCGGG 1 cut(s) 1363
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 6 cut(s) 112, 142, 761, 861, 906, 1709
CspCI CAANNNNNGTGG 2 cut(s) 1249, 1284
CviQI GTAC 6 cut(s) 112, 142, 761, 861, 906, 1709
DdeI CTNAG 4 cut(s) 534, 655, 985, 1386
DpnI GATC 7 cut(s) 310, 777, 793, 915, 1330, 1459, 1470
DpnII GATC 7 cut(s) 308, 775, 791, 913, 1328, 1457, 1468
EaeI YGGCCR 1 cut(s) 556
Eam1104I CTCTTC 1 cut(s) 1653
EarI CTCTTC 1 cut(s) 1653
Ecl136II GAGCTC 1 cut(s) 17
Eco130I CCWWGG 3 cut(s) 559, 628, 881
Eco24I GRGCYC 1 cut(s) 19
Eco32I GATATC 2 cut(s) 370, 724
Eco47I GGWCC 2 cut(s) 437, 848
Eco53kI GAGCTC 1 cut(s) 17
Eco57I CTGAAG 2 cut(s) 1188, 1650
Eco88I CYCGRG 1 cut(s) 1363
EcoICRI GAGCTC 1 cut(s) 17
EcoO109I RGGNCCY 1 cut(s) 1108
EcoRII CCWGG 3 cut(s) 21, 114, 1111
EcoRV GATATC 2 cut(s) 370, 724
EcoT14I CCWWGG 3 cut(s) 559, 628, 881
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 3 cut(s) 559, 628, 881
FalI AAGNNNNNCTT 2 cut(s) 271, 303
FaqI GGGAC 3 cut(s) 23, 329, 952
FauNDI CATATG 1 cut(s) 1245
FbaI TGATCA 1 cut(s) 1468
FblI GTMKAC 3 cut(s) 539, 973, 1195
FokI GGATG 5 cut(s) 514, 560, 617, 764, 1763
FriOI GRGCYC 1 cut(s) 19
FspBI CTAG 3 cut(s) 249, 852, 858
GsuI CTGGAG 1 cut(s) 5
HaeIII GGCC 5 cut(s) 403, 558, 1109, 1554, 1577
HapII CCGG 6 cut(s) 229, 588, 749, 1203, 1364, 1590
HindIII AAGCTT 1 cut(s) 1380
HinfI GANTC 3 cut(s) 389, 407, 1750
HpaII CCGG 6 cut(s) 229, 588, 749, 1203, 1364, 1590
HphI GGTGA 2 cut(s) 138, 1131
Hpy166II GTNNAC 6 cut(s) 112, 142, 540, 974, 1033, 1196
Hpy188I TCNGA 7 cut(s) 412, 791, 918, 1450, 1545, 1702, 1744
Hpy188III TCNNGA 6 cut(s) 128, 393, 1203, 1313, 1332, 1419
Hpy8I GTNNAC 6 cut(s) 112, 142, 540, 974, 1033, 1196
HpyAV CCTTC 2 cut(s) 1096, 1651
HpyCH4III ACNGT 6 cut(s) 760, 865, 895, 992, 1193, 1681
HpyF10VI GCNNNNNNNGC 8 cut(s) 581, 593, 665, 677, 686, 1127, 1234, 1443
HpyF3I CTNAG 4 cut(s) 534, 655, 985, 1386
Kpn2I TCCGGA 1 cut(s) 1202
KroI GCCGGC 1 cut(s) 1589
KroNI GCCGGC 1 cut(s) 1591
Ksp22I TGATCA 1 cut(s) 1468
Kzo9I GATC 7 cut(s) 308, 775, 791, 913, 1328, 1457, 1468
LmnI GCTCC 1 cut(s) 105
LweI GCATC 3 cut(s) 691, 1051, 1736
MaeI CTAG 3 cut(s) 249, 852, 858
MaeIII GTNAC 5 cut(s) 120, 754, 865, 885, 1675
MalI GATC 7 cut(s) 310, 777, 793, 915, 1330, 1459, 1470
MbiI CCGCTC 1 cut(s) 1310
MboI GATC 7 cut(s) 308, 775, 791, 913, 1328, 1457, 1468
MboII GAAGA 7 cut(s) 58, 61, 1181, 1403, 1670, 1675, 1715
MfeI CAATTG 1 cut(s) 780
MflI RGATCY 5 cut(s) 308, 791, 913, 1328, 1457
MhlI GDGCHC 2 cut(s) 19, 514
MlsI TGGCCA 1 cut(s) 558
MluNI TGGCCA 1 cut(s) 558
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 4 cut(s) 316, 367, 769, 1666
MnlI CCTC 9 cut(s) 130, 450, 453, 541, 586, 635, 664, 1170, 1219
Mox20I TGGCCA 1 cut(s) 558
MroI TCCGGA 1 cut(s) 1202
MroNI GCCGGC 1 cut(s) 1589
MroXI GAANNNNTTC 1 cut(s) 923
MscI TGGCCA 1 cut(s) 558
MseI TTAA 5 cut(s) 953, 1002, 1251, 1794, 1798
MslI CAYNNNNRTG 1 cut(s) 1619
Msp20I TGGCCA 1 cut(s) 558
MspA1I CMGCKG 1 cut(s) 1493
MspI CCGG 6 cut(s) 229, 588, 749, 1203, 1364, 1590
MspR9I CCNGG 8 cut(s) 23, 116, 230, 589, 749, 1113, 1364, 1365
MunI CAATTG 1 cut(s) 780
Mva1269I GAATGC 1 cut(s) 598
MvaI CCWGG 3 cut(s) 23, 116, 1113
MwoI GCNNNNNNNGC 8 cut(s) 581, 593, 665, 677, 686, 1127, 1234, 1443
NaeI GCCGGC 1 cut(s) 1591
NciI CCSGG 5 cut(s) 230, 589, 749, 1364, 1365
NcoI CCATGG 2 cut(s) 559, 881
NdeI CATATG 1 cut(s) 1245
NdeII GATC 7 cut(s) 308, 775, 791, 913, 1328, 1457, 1468
NgoMIV GCCGGC 1 cut(s) 1589
NheI GCTAGC 1 cut(s) 248
NlaIV GGNNCC 3 cut(s) 1110, 1207, 1626
NmeAIII GCCGAG 1 cut(s) 812
NmuCI GTSAC 2 cut(s) 120, 1675
NspI RCATGY 2 cut(s) 1184, 1721
PacI TTAATTAA 1 cut(s) 1798
PasI CCCWGGG 1 cut(s) 1112
PciI ACATGT 1 cut(s) 1717
PctI GAATGC 1 cut(s) 598
PdiI GCCGGC 1 cut(s) 1591
PdmI GAANNNNTTC 1 cut(s) 923
PfeI GAWTC 2 cut(s) 407, 1750
PflMI CCANNNNNTGG 2 cut(s) 453, 805
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
PscI ACATGT 1 cut(s) 1717
PshBI ATTAAT 1 cut(s) 953
PsiI TTATAA 1 cut(s) 1302
Psp124BI GAGCTC 1 cut(s) 19
Psp6I CCWGG 3 cut(s) 21, 114, 1111
PspGI CCWGG 3 cut(s) 21, 114, 1111
PspN4I GGNNCC 3 cut(s) 1110, 1207, 1626
PspPI GGNCC 3 cut(s) 437, 848, 1108
PstI CTGCAG 1 cut(s) 771
PstNI CAGNNNCTG 2 cut(s) 1448, 1496
PsuI RGATCY 5 cut(s) 308, 791, 913, 1328, 1457
PvuII CAGCTG 1 cut(s) 1493
RsaI GTAC 6 cut(s) 113, 143, 762, 862, 907, 1710
RsaNI GTAC 6 cut(s) 112, 142, 761, 861, 906, 1709
RseI CAYNNNNRTG 1 cut(s) 1619
SacI GAGCTC 1 cut(s) 19
SaqAI TTAA 5 cut(s) 953, 1002, 1251, 1794, 1798
Sau3AI GATC 7 cut(s) 308, 775, 791, 913, 1328, 1457, 1468
Sau96I GGNCC 3 cut(s) 437, 848, 1108
ScaI AGTACT 1 cut(s) 907
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 8 cut(s) 23, 116, 230, 589, 749, 1113, 1364, 1365
SduI GDGCHC 2 cut(s) 19, 514
SfaNI GCATC 3 cut(s) 691, 1051, 1736
SfcI CTRYAG 4 cut(s) 767, 909, 1356, 1440
SinI GGWCC 2 cut(s) 437, 848
SmaI CCCGGG 1 cut(s) 1365
SmiMI CAYNNNNRTG 1 cut(s) 1619
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
SsiI CCGC 1 cut(s) 1308
SspI AATATT 1 cut(s) 1584
SspMI CTAG 3 cut(s) 249, 852, 858
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 8 cut(s) 21, 114, 228, 587, 747, 1111, 1362, 1363
StyI CCWWGG 3 cut(s) 559, 628, 881
TaaI ACNGT 6 cut(s) 760, 865, 895, 992, 1193, 1681
TaqI TCGA 1 cut(s) 84
TatI WGTACW 4 cut(s) 141, 760, 905, 1708
TauI GCSGC 1 cut(s) 1310
TfiI GAWTC 2 cut(s) 407, 1750
Tru1I TTAA 5 cut(s) 953, 1002, 1251, 1794, 1798
Tru9I TTAA 5 cut(s) 953, 1002, 1251, 1794, 1798
TscAI CASTG 2 cut(s) 577, 1236
TseFI GTSAC 2 cut(s) 120, 1675
Tsp45I GTSAC 2 cut(s) 120, 1675
TspDTI ATGAA 8 cut(s) 102, 564, 1295, 1392, 1509, 1620, 1671, 1742
TspMI CCCGGG 1 cut(s) 1363
TspRI CASTG 2 cut(s) 577, 1236
Van91I CCANNNNNTGG 2 cut(s) 453, 805
VpaK11BI GGWCC 2 cut(s) 437, 848
VspI ATTAAT 1 cut(s) 953
XapI RAATTY 4 cut(s) 481, 1282, 1316, 1526
XceI RCATGY 2 cut(s) 1184, 1721
XmaI CCCGGG 1 cut(s) 1363
XmiI GTMKAC 3 cut(s) 539, 973, 1195
XmnI GAANNNNTTC 1 cut(s) 923
XspI CTAG 3 cut(s) 249, 852, 858
ZrmI AGTACT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.