Prupe.6G067700_v2.0.a1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000257
Physical Location & Seq
Reverse (-)
4657222 .. 4660801
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G067700.1

Sequence Viewer

Length: 2217 bp
ATGGCTAAGCATTTAGCACTTCTGTTTTATCATGCCTTCACCATTCTCATCCTAACTATGAGTTTATTGTCCAAAGCCACTTCTGAAGATAGAGAGGTCTCTATTGTGTACTTGGGATCACTTCCTCATGGAGTGTACTCTCCATTGTCTCACCACCACAGTATTCTACAGAGAGTTATCCAGGGAAGTTCTCTTGAAAGTTTATTAGTGAGAAGTTACAAACGGAGTTTCAATGGATTCGCTGCCAAGCTCACTGACCAGGAAAGAGAAAAACTTGCAAACATGAAGGAAGTGGTCTCTGTCTTTCCAAGCAGAACATATCAACTCCAAACAACAAGATCTTGGGACTTTATGGGTTTCGATGAGAAAATCAATCGAAATGCCACTGTTGAGAGTGACATTATTGTTGGTGTAATTGATAGTGGAATTTGGCCTGAATCAGAGAGCTTTACAGATGTAGGTTTTGGTCCTGCTCCTAAGAGGTGGAAAGGTGCTTGTAAAGGTGGCAAAAATTTCACTTGCAACAACAAACTCATTGGAGCTCGATATTACACAGCAGAGTCTGCAAGGGATGGTACAGGTCATGGAACCCACACTGCCTCAACGGCAGCTGGGAATGGCGTAAAGGATGCAAGCTTTTATGGACTAGCACAAGGTACTGCTAGAGGAGGAGTACCGTCTGCGAGAATTGCAGCATATAAAGTTTGTGCTCCAACGTGCATGGCACACAATATTTTGGCCGCTTTTGACGATGCTATTAATGATGGAGTTGACATTATTACCATTTCAATTGGACTTGGTTTTATGGAAGATGTCTATGGGGATGCTATTTCCATTGGTGCTTTTCATGCAATGGAAAATGGGATACTAACCACAAACTCTGCCGGCAACAATGGTCCTTCAGATGGTACTGTGTCAAGTCCAGCTCCCTGGATATTGACAGTTGCAGCAAGTAGCATAGATCGTCGGATCATTGACAAAGTTGTTTTTGGAATGGGTACAACAGTAGTTGGGAATTCTGTGAACTCTTTTACATTAAATGGAACAAGTTTCCCTCTGGTACATGGAAAAGATGTTTCAAGTAATTGCACAGAGGAAGACGCAGGATATTGTAAAGAAGGTTGCCTGGACAGTCAATTAGTAAAGGGAAAGATTGTGCTATGTGATCGATACACTAGTGCAATTCCTGAGGCGTATAAAGCTGGCGCACTAGGTTCAGTTGTACTTAATTTCTACAACGATGATGATCTTTCTTTGATTCTCCCATTAGCTGCGACGGCTTTACACCCGAAAGAGTATAGTGTGATGATGTCCTACATGAACTCCACTAGAGATCCTCAAGGAACCATACTAAAAAGTGAACACATAAAAGATCCTGCTGCACCTCATGTCGCTTTCTTCTCTTCACGTGGACCAAATCTAATTTTACCGGAAATTATCAAGCCGGATATAAGTGCCCCAGGAGTACAAATTCTGGCTGCATATTCGCCTGATGCTTCCATCTCAGAGTTTGAAGACAAGAGGCATGTAAAATACAAGATCATGTCTGGAACCTCCATGGCTTGCCCCCATGCTGCTGGCGTAGCTGCATACGTTAAAAGCTTCCACCCTGACTGGTCTCCGGCCACCATCAAATCATCCCTTATGACTACTGCTTGGCCCATGAATGATACCAGCACCAAGAGCAACATTTCCACTGGTGAATTTGCTTATGGATCGGGACACATCAATCCGGTAAAAGCTATAAACCCGGGGCTAGTTTATGAAGCTTCAAAAGAAGACTACATAAGGTTGCTATGCATGAAATATGATGAAGGCAAAGTTAGACTTGTATCAGGTGATAACAGCACATGCCCTACAGATAAAGGGTCACCACTGGATCATAACTACCCTTCAATGGCAGCTAAAGTTACCCCAATGAATCCTTTCGCGGTTAAATTTCATAGAAGAGTTAAAAATGTTGGCCTTGCAAACTCCAGTTACAAGGCCACAATCTTTCAAGACTCCAAAGTTGAGATCAAAGTGGTGCCTGAAATTCTTTCCTTCAAGTCCTTGAATGAGGAGAAGGCTTTTGATGTGACTGTGGCTGGAAGCGGTGTGCCTTTTGGATCACAAGTTTCTACACAGCTGGTTTGGTTTGATGGTGCTCACAGTGTTAGAAGCCCAATTGTTGTATACACTGCAGCTATGCAAATGCAGGATCAATATCCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

739

Amino Acids

79.63

Weight (kDa)

6.6

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2026
AccI GTMKAC 1 cut(s) 2175
AccII CGCG 1 cut(s) 1931
AciI CCGC 3 cut(s) 741, 1931, 2094
AclWI GGATC 8 cut(s) 124, 977, 1328, 1367, 1723, 1887, 2116, 2208
AcoI YGGCCR 2 cut(s) 738, 1623
AcsI RAATTY 7 cut(s) 426, 511, 1015, 1470, 1703, 1937, 2034
AcuI CTGAAG 2 cut(s) 105, 885
AcvI CACGTG 1 cut(s) 1409
AfiI CCNNNNNNNGG 2 cut(s) 905, 1897
AhlI ACTAGT 1 cut(s) 1175
AjnI CCWGG 5 cut(s) 180, 258, 929, 1125, 1459
Alw21I GWGCWC 3 cut(s) 544, 712, 2149
Alw26I GTCTC 4 cut(s) 103, 153, 301, 1623
AlwI GGATC 8 cut(s) 124, 977, 1328, 1367, 1723, 1887, 2116, 2208
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 1750
AoxI GGCC 6 cut(s) 431, 738, 1623, 1658, 1963, 1986
ApoI RAATTY 7 cut(s) 426, 511, 1015, 1470, 1703, 1937, 2034
AseI ATTAAT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 1925
AspLEI GCGC 1 cut(s) 1208
AspS9I GGNCC 4 cut(s) 467, 896, 1412, 1659
AsuC2I CCSGG 2 cut(s) 1751, 1752
AsuHPI GGTGA 5 cut(s) 31, 143, 1712, 1850, 1863
AvaI CYCGRG 1 cut(s) 1750
AvaII GGWCC 3 cut(s) 467, 896, 1412
AxyI CCTNAGG 1 cut(s) 1188
BaeGI GKGCMC 1 cut(s) 1459
BaeI ACNNNNGTAYC 4 cut(s) 990, 1023, 1162, 1195
BanI GGYRCC 1 cut(s) 2026
BanII GRGCYC 1 cut(s) 544
BbrPI CACGTG 1 cut(s) 1409
BbsI GAAGAC 3 cut(s) 1104, 1521, 1785
Bbv12I GWGCWC 3 cut(s) 544, 712, 2149
BccI CCATC 6 cut(s) 566, 758, 899, 1508, 1637, 2135
BceAI ACGGC 2 cut(s) 621, 1293
BciT130I CCWGG 5 cut(s) 182, 260, 931, 1127, 1461
BciVI GTATCC 1 cut(s) 858
BcnI CCSGG 2 cut(s) 1751, 1752
BcoDI GTCTC 4 cut(s) 103, 153, 301, 1623
BcuI ACTAGT 1 cut(s) 1175
BfaI CTAG 6 cut(s) 647, 663, 1176, 1211, 1329, 1757
BfmI CTRYAG 3 cut(s) 167, 1857, 2181
BfuI GTATCC 1 cut(s) 858
BglI GCCNNNNNGGC 1 cut(s) 605
BglII AGATCT 1 cut(s) 338
BlpI GCTNAGC 1 cut(s) 6
Bme1390I CCNGG 7 cut(s) 182, 260, 931, 1127, 1461, 1751, 1752
Bme18I GGWCC 3 cut(s) 467, 896, 1412
BmeT110I CYCGRG 1 cut(s) 1750
BmgT120I GGNCC 4 cut(s) 467, 896, 1412, 1659
BmiI GGNNCC 4 cut(s) 589, 1345, 1552, 2028
BmrFI CCNGG 7 cut(s) 182, 260, 931, 1127, 1461, 1751, 1752
BmsI GCATC 4 cut(s) 619, 742, 814, 1483
BpiI GAAGAC 3 cut(s) 1104, 1521, 1785
BpmI CTGGAG 1 cut(s) 1960
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 1323
BpuMI CCSGG 2 cut(s) 1751, 1752
Bsa29I ATCGAT 1 cut(s) 1168
BsaAI YACGTR 1 cut(s) 1409
BsaBI GATNNNNATC 2 cut(s) 1245, 2205
BsaI GGTCTC 3 cut(s) 103, 301, 1623
BsaJI CCNNGG 6 cut(s) 181, 929, 1459, 1557, 1750, 1751
BsaWI WCCGGW 2 cut(s) 1429, 1732
BsaXI ACNNNNNCTCC 2 cut(s) 1307, 1337
Bsc4I CCNNNNNNNGG 2 cut(s) 905, 1897
Bse118I RCCGGY 1 cut(s) 884
Bse1I ACTGG 4 cut(s) 1619, 1702, 1881, 1977
Bse21I CCTNAGG 1 cut(s) 1188
Bse3DI GCAATG 1 cut(s) 858
Bse8I GATNNNNATC 2 cut(s) 1245, 2205
BseBI CCWGG 5 cut(s) 182, 260, 931, 1127, 1461
BseCI ATCGAT 1 cut(s) 1168
BseDI CCNNGG 6 cut(s) 181, 929, 1459, 1557, 1750, 1751
BseGI GGATG 5 cut(s) 48, 577, 634, 829, 1637
BseJI GATNNNNATC 2 cut(s) 1245, 2205
BseLI CCNNNNNNNGG 2 cut(s) 905, 1897
BseMI GCAATG 1 cut(s) 858
BseMII CTCAG 2 cut(s) 1179, 1518
BseNI ACTGG 4 cut(s) 1619, 1702, 1881, 1977
BseRI GAGGAG 3 cut(s) 681, 684, 2075
BseSI GKGCMC 1 cut(s) 1459
BseYI CCCAGC 1 cut(s) 611
BsgI GTGCAG 1 cut(s) 1365
Bsh1236I CGCG 1 cut(s) 1931
BshFI GGCC 6 cut(s) 433, 740, 1625, 1660, 1965, 1988
BshNI GGYRCC 1 cut(s) 2026
BshVI ATCGAT 1 cut(s) 1168
BsiHKAI GWGCWC 3 cut(s) 544, 712, 2149
BsiHKCI CYCGRG 1 cut(s) 1750
BsiSI CCGG 6 cut(s) 885, 1430, 1445, 1622, 1733, 1751
BslFI GGGAC 2 cut(s) 359, 1734
BslI CCNNNNNNNGG 2 cut(s) 905, 1897
BsmAI GTCTC 4 cut(s) 103, 153, 301, 1623
BsmFI GGGAC 2 cut(s) 359, 1734
BsnI GGCC 6 cut(s) 433, 740, 1625, 1660, 1965, 1988
Bso31I GGTCTC 3 cut(s) 103, 301, 1623
BsoBI CYCGRG 1 cut(s) 1750
Bsp1286I GDGCHC 4 cut(s) 544, 712, 1459, 2149
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 1557
BspACI CCGC 3 cut(s) 741, 1931, 2094
BspANI GGCC 6 cut(s) 433, 740, 1625, 1660, 1965, 1988
BspCNI CTCAG 2 cut(s) 1180, 1517
BspDI ATCGAT 1 cut(s) 1168
BspFNI CGCG 1 cut(s) 1931
BspLI GGNNCC 4 cut(s) 589, 1345, 1552, 2028
BspMAI CTGCAG 1 cut(s) 2185
BspPI GGATC 8 cut(s) 124, 977, 1328, 1367, 1723, 1887, 2116, 2208
BspT107I GGYRCC 1 cut(s) 2026
BspTNI GGTCTC 3 cut(s) 103, 301, 1623
BsrDI GCAATG 1 cut(s) 858
BsrFI RCCGGY 1 cut(s) 884
BsrI ACTGG 4 cut(s) 1619, 1702, 1881, 1977
BssAI RCCGGY 1 cut(s) 884
BssECI CCNNGG 6 cut(s) 181, 929, 1459, 1557, 1750, 1751
BssNAI GTATAC 1 cut(s) 2176
BssT1I CCWWGG 1 cut(s) 1557
Bst1107I GTATAC 1 cut(s) 2176
Bst2UI CCWGG 5 cut(s) 182, 260, 931, 1127, 1461
Bst4CI ACNGT 9 cut(s) 161, 388, 678, 913, 943, 1006, 1133, 2083, 2153
Bst6I CTCTTC 2 cut(s) 1408, 1942
BstAPI GCANNNNNTGC 1 cut(s) 563
BstBAI YACGTR 1 cut(s) 1409
BstC8I GCNNGC 5 cut(s) 634, 886, 1204, 1564, 1579
BstDEI CTNAG 4 cut(s) 6, 477, 1188, 1504
BstDSI CCRYGG 1 cut(s) 1557
BstEII GGTNACC 1 cut(s) 1869
BstF5I GGATG 5 cut(s) 48, 577, 634, 829, 1637
BstFNI CGCG 1 cut(s) 1931
BstHHI GCGC 1 cut(s) 1208
BstMAI GTCTC 4 cut(s) 103, 153, 301, 1623
BstMWI GCNNNNNNNGC 8 cut(s) 563, 605, 689, 848, 1199, 1277, 1583, 1683
BstNI CCWGG 5 cut(s) 182, 260, 931, 1127, 1461
BstNSI RCATGY 2 cut(s) 1529, 1854
BstPI GGTNACC 1 cut(s) 1869
BstSCI CCNGG 7 cut(s) 180, 258, 929, 1125, 1459, 1749, 1750
BstSFI CTRYAG 3 cut(s) 167, 1857, 2181
BstSLI GKGCMC 1 cut(s) 1459
BstUI CGCG 1 cut(s) 1931
BstV2I GAAGAC 3 cut(s) 1104, 1521, 1785
BstX2I RGATCY 3 cut(s) 338, 1333, 1372
BstXI CCANNNNNNTGG 2 cut(s) 930, 1577
BstYI RGATCY 3 cut(s) 338, 1333, 1372
BstZ17I GTATAC 1 cut(s) 2176
Bsu15I ATCGAT 1 cut(s) 1168
Bsu36I CCTNAGG 1 cut(s) 1188
BsuI GTATCC 1 cut(s) 858
BsuRI GGCC 6 cut(s) 433, 740, 1625, 1660, 1965, 1988
BsuTUI ATCGAT 1 cut(s) 1168
BtgI CCRYGG 1 cut(s) 1557
BtsCI GGATG 5 cut(s) 48, 577, 634, 829, 1637
BtsI GCAGTG 2 cut(s) 594, 2178
BtsIMutI CAGTG 7 cut(s) 252, 384, 594, 1695, 1874, 2158, 2178
Cac8I GCNNGC 5 cut(s) 634, 886, 1204, 1564, 1579
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 1 cut(s) 1208
Cfr10I RCCGGY 1 cut(s) 884
Cfr13I GGNCC 4 cut(s) 467, 896, 1412, 1659
Cfr9I CCCGGG 1 cut(s) 1750
ClaI ATCGAT 1 cut(s) 1168
CseI GACGC 1 cut(s) 1109
DdeI CTNAG 4 cut(s) 6, 477, 1188, 1504
EaeI YGGCCR 2 cut(s) 738, 1623
Eam1104I CTCTTC 2 cut(s) 1408, 1942
EarI CTCTTC 2 cut(s) 1408, 1942
Ecl136II GAGCTC 1 cut(s) 542
Eco130I CCWWGG 1 cut(s) 1557
Eco24I GRGCYC 1 cut(s) 544
Eco31I GGTCTC 3 cut(s) 103, 301, 1623
Eco47I GGWCC 3 cut(s) 467, 896, 1412
Eco53kI GAGCTC 1 cut(s) 542
Eco57I CTGAAG 2 cut(s) 105, 885
Eco72I CACGTG 1 cut(s) 1409
Eco81I CCTNAGG 1 cut(s) 1188
Eco88I CYCGRG 1 cut(s) 1750
Eco91I GGTNACC 1 cut(s) 1869
EcoICRI GAGCTC 1 cut(s) 542
EcoO65I GGTNACC 1 cut(s) 1869
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 5 cut(s) 180, 258, 929, 1125, 1459
EcoT14I CCWWGG 1 cut(s) 1557
EcoT22I ATGCAT 1 cut(s) 1802
EcoT38I GRGCYC 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 1557
FalI AAGNNNNNCTT 2 cut(s) 1812, 1844
FaqI GGGAC 2 cut(s) 359, 1734
FblI GTMKAC 1 cut(s) 2175
FokI GGATG 5 cut(s) 35, 584, 641, 836, 1624
FriOI GRGCYC 1 cut(s) 544
FspBI CTAG 6 cut(s) 647, 663, 1176, 1211, 1329, 1757
GlaI GCGC 1 cut(s) 1207
GsaI CCCAGC 1 cut(s) 615
GsuI CTGGAG 1 cut(s) 1960
HaeIII GGCC 6 cut(s) 433, 740, 1625, 1660, 1965, 1988
HapII CCGG 6 cut(s) 885, 1430, 1445, 1622, 1733, 1751
HgaI GACGC 1 cut(s) 1109
HhaI GCGC 1 cut(s) 1208
Hin6I GCGC 1 cut(s) 1206
HinP1I GCGC 1 cut(s) 1206
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HindIII AAGCTT 3 cut(s) 634, 1600, 1767
HinfI GANTC 6 cut(s) 237, 437, 560, 1258, 1921, 2003
HpaII CCGG 6 cut(s) 885, 1430, 1445, 1622, 1733, 1751
HphI GGTGA 5 cut(s) 31, 143, 1712, 1850, 1863
Hpy166II GTNNAC 7 cut(s) 109, 136, 772, 1024, 1361, 1412, 2176
Hpy188I TCNGA 5 cut(s) 85, 442, 904, 969, 1507
Hpy188III TCNNGA 5 cut(s) 194, 1187, 1548, 1719, 2000
Hpy8I GTNNAC 7 cut(s) 109, 136, 772, 1024, 1361, 1412, 2176
Hpy99I CGWCG 2 cut(s) 969, 1279
HpyAV CCTTC 8 cut(s) 46, 280, 909, 1112, 1808, 1902, 2053, 2059
HpyCH4III ACNGT 9 cut(s) 161, 388, 678, 913, 943, 1006, 1133, 2083, 2153
HpyCH4IV ACGT 3 cut(s) 716, 1408, 1593
HpyF10VI GCNNNNNNNGC 8 cut(s) 563, 605, 689, 848, 1199, 1277, 1583, 1683
HpyF3I CTNAG 4 cut(s) 6, 477, 1188, 1504
HpySE526I ACGT 3 cut(s) 716, 1408, 1593
HspAI GCGC 1 cut(s) 1206
KroI GCCGGC 1 cut(s) 884
KroNI GCCGGC 1 cut(s) 886
LmnI GCTCC 4 cut(s) 478, 539, 715, 931
LweI GCATC 4 cut(s) 619, 742, 814, 1483
MaeI CTAG 6 cut(s) 647, 663, 1176, 1211, 1329, 1757
MaeII ACGT 3 cut(s) 716, 1408, 1593
MaeIII GTNAC 6 cut(s) 215, 395, 1869, 1909, 1979, 2077
MboII GAAGA 8 cut(s) 98, 821, 1109, 1390, 1395, 1526, 1790, 1959
MfeI CAATTG 2 cut(s) 789, 2166
MflI RGATCY 3 cut(s) 338, 1333, 1372
MhlI GDGCHC 4 cut(s) 544, 712, 1459, 2149
MlyI GAGTC 2 cut(s) 569, 1997
MmeI TCCRAC 2 cut(s) 737, 947
Mph1103I ATGCAT 1 cut(s) 1802
MroNI GCCGGC 1 cut(s) 884
MroXI GAANNNNTTC 1 cut(s) 1925
MseI TTAA 7 cut(s) 759, 1037, 1227, 1596, 1935, 1953, 2215
MspA1I CMGCKG 2 cut(s) 611, 2128
MspI CCGG 6 cut(s) 885, 1430, 1445, 1622, 1733, 1751
MspR9I CCNGG 7 cut(s) 182, 260, 931, 1127, 1461, 1751, 1752
MunI CAATTG 2 cut(s) 789, 2166
MvaI CCWGG 5 cut(s) 182, 260, 931, 1127, 1461
MvnI CGCG 1 cut(s) 1931
MwoI GCNNNNNNNGC 8 cut(s) 563, 605, 689, 848, 1199, 1277, 1583, 1683
NaeI GCCGGC 1 cut(s) 886
NciI CCSGG 2 cut(s) 1751, 1752
NcoI CCATGG 1 cut(s) 1557
NgoMIV GCCGGC 1 cut(s) 884
NlaIV GGNNCC 4 cut(s) 589, 1345, 1552, 2028
NmuCI GTSAC 3 cut(s) 395, 1869, 2077
NsiI ATGCAT 1 cut(s) 1802
NspI RCATGY 2 cut(s) 1529, 1854
PdiI GCCGGC 1 cut(s) 886
PdmI GAANNNNTTC 1 cut(s) 1925
PfeI GAWTC 4 cut(s) 237, 437, 1258, 1921
PleI GAGTC 2 cut(s) 568, 1997
PmaCI CACGTG 1 cut(s) 1409
PmlI CACGTG 1 cut(s) 1409
PpsI GAGTC 2 cut(s) 568, 1997
Ppu21I YACGTR 1 cut(s) 1409
PshBI ATTAAT 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 544
Psp6I CCWGG 5 cut(s) 180, 258, 929, 1125, 1459
PspCI CACGTG 1 cut(s) 1409
PspEI GGTNACC 1 cut(s) 1869
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 5 cut(s) 180, 258, 929, 1125, 1459
PspN4I GGNNCC 4 cut(s) 589, 1345, 1552, 2028
PspPI GGNCC 4 cut(s) 467, 896, 1412, 1659
PstI CTGCAG 1 cut(s) 2185
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 3 cut(s) 338, 1333, 1372
PvuII CAGCTG 2 cut(s) 611, 2128
SacI GAGCTC 1 cut(s) 544
SaqAI TTAA 7 cut(s) 759, 1037, 1227, 1596, 1935, 1953, 2215
Sau96I GGNCC 4 cut(s) 467, 896, 1412, 1659
SchI GAGTC 2 cut(s) 569, 1997
ScrFI CCNGG 7 cut(s) 182, 260, 931, 1127, 1461, 1751, 1752
SduI GDGCHC 4 cut(s) 544, 712, 1459, 2149
SfaNI GCATC 4 cut(s) 619, 742, 814, 1483
SfcI CTRYAG 3 cut(s) 167, 1857, 2181
SinI GGWCC 3 cut(s) 467, 896, 1412
SmaI CCCGGG 1 cut(s) 1752
SmlI CTYRAG 1 cut(s) 1338
SmoI CTYRAG 1 cut(s) 1338
SpeI ACTAGT 1 cut(s) 1175
SsiI CCGC 3 cut(s) 741, 1931, 2094
SspI AATATT 1 cut(s) 733
SspMI CTAG 6 cut(s) 647, 663, 1176, 1211, 1329, 1757
SstI GAGCTC 1 cut(s) 544
StyD4I CCNGG 7 cut(s) 180, 258, 929, 1125, 1459, 1749, 1750
StyI CCWWGG 1 cut(s) 1557
TaaI ACNGT 9 cut(s) 161, 388, 678, 913, 943, 1006, 1133, 2083, 2153
TaiI ACGT 3 cut(s) 719, 1411, 1596
TaqI TCGA 4 cut(s) 360, 376, 544, 1168
TatI WGTACW 4 cut(s) 108, 135, 1222, 1465
TauI GCSGC 1 cut(s) 743
TfiI GAWTC 4 cut(s) 237, 437, 1258, 1921
Tru1I TTAA 7 cut(s) 759, 1037, 1227, 1596, 1935, 1953, 2215
Tru9I TTAA 7 cut(s) 759, 1037, 1227, 1596, 1935, 1953, 2215
TscAI CASTG 7 cut(s) 259, 391, 601, 1702, 1881, 2158, 2185
TseFI GTSAC 3 cut(s) 395, 1869, 2077
Tsp45I GTSAC 3 cut(s) 395, 1869, 2077
TspDTI ATGAA 9 cut(s) 299, 836, 1334, 1679, 1779, 1817, 1827, 1931, 1934
TspGWI ACGGA 1 cut(s) 238
TspMI CCCGGG 1 cut(s) 1750
TspRI CASTG 7 cut(s) 259, 391, 601, 1702, 1881, 2158, 2185
VpaK11BI GGWCC 3 cut(s) 467, 896, 1412
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 7 cut(s) 426, 511, 1015, 1470, 1703, 1937, 2034
XceI RCATGY 2 cut(s) 1529, 1854
XmaI CCCGGG 1 cut(s) 1750
XmiI GTMKAC 1 cut(s) 2175
XmnI GAANNNNTTC 1 cut(s) 1925
XspI CTAG 6 cut(s) 647, 663, 1176, 1211, 1329, 1757
Zsp2I ATGCAT 1 cut(s) 1802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.