pycom02g24440

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000257
Physical Location & Seq
Forward (+)
22616999 .. 22620506
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g24440.1

Sequence Viewer

Length: 2496 bp
ATGATGGACGCCATGGCCAACATTGCCATCCGATACCTCTGTGACTTAGGGAAGATGTCGAGTTTTTACGCCAATTTGGCGGGAAGAACCAAGTCTAATATGTTTGATATTATTAGGGTGCTGGAGGATTTGGAGTCATCGCAAGGGTTTTTGGGTGCGGCAGAGATGGGTCATTGTCTTACAGAGTTCGGGATTGTAAGAGGCATTGTGGAGTATGTAGGTTCGGCTGAGGAGATCCCCTTTGCACACCCGAATTTCGAGCAAATTGGGGATGCACCATCGAGTAAGCATTTGCCCAATTGGTTGCCGGGAAGGGTGAGGGTATTGACAAATCAAAATTGGATAGGATCTTATTCCAAATTTAAACATTTGGAATTGTTCATCCTGCATGGAAAGAAATTAAATTTCATGCCATGGAAATTGTGGTCAAGGGATAAAATCGTCTGGATATCAGTGAATAAGATTCTTGTTGTCCTTCATCCAAATATAAAAGACTGGCCAGTGGTTACGATCGTTCAGCATCAAAACAAAACAATGGCAAAGCGCGGAGATCTCCTGGTTTCTTATGCTTTGTCCATTATTTTCATACTCGTCGTGAGCTTACCGTGTGAAGCCGTCGATCATGGCGATAGAAAGACATATATTGTGTACTTGGGATCACTTCCTAAGGATGACGTGTTCTCGCCATTGTCTCATCACATTGGTATTCTCGAAAGGGTTACCGATAGTAGCTCTGCTTCGAATCTATTGGTAAGAAGCTACAAAAGAAGCTTCAACGGATTCGCTGCAAAGCTCACCGACCGAGAAAGAGAAAGGCTTGCTAAGATGAAGGAAGTTGTCTCTTTTTTGGGTTTCAATGAGAAACTCAAGCGTAATCCTACTGTCGAGAGCAATGTTATTGTTGGTGTCATCGATACTGGGATATGGCCTGAATCGGAAAGTTTTAGCGATGAAGGTTTTGGTCCAGCTCCAAAGAAGTGGAAAGGAGTTTGCAAAGGAGGCAAAAATTTCACTTGCAACAAGACGATCATTGGAGCTCGGTTTTACGTTGGTTCCTCAGCTAGAGATGAGATTGGTCATGGAAGTCATACTGCCTCAACCGTAGCAGGAATTCCGGTAAAGGGTGTGAGCTTTTACGGACTGGCGCACGGAACTGCAACAGGAGGCGTTCCCTCTGCGAGAATTGCTGAATATAAAGTTTGCGGTTCTAACGAGTGTGCTACAGACAAGATATTGGCTGCTTTTGATGATGCAATTGCTGATGGAGTTGACATCATCACAATTTCAATTGGAGACTCAATTGTAGTCCCTTTCGATCAGGATCCTATAGCAATCGGTGCTTTTCATGCACTGGAGAAAGGGATACTAACCTCTCAGTCTGCAGGGAACGACGGCCCTTATATGGGTACTGTATCAAGTGTAGCACCATGGATTCTTACAGTTGCAGCAAGTAGCATAGACCGTCACATCATTGACAAGGTTGTTCTTGGAAACGGAAGGACACTTGTCGGGAATTCTGTGAACGCTTTTGATTTAAATGGAACAAGTTTTCCCTTGATAGAAGGCAAAGACGCGTCGAGTGAATGCGGCGATTGTGATGAAGGTTGCCTAGACCCTAACTTAGTTAAGGGAAAGATTGTGGTATGTCGTTGGCCTATAGGCATTATCGAGGCTCGTCGAGTTGGAGCAATAGGTGCAGTAACTGACAGTACCATACCCGATGTTTCTCCCATTGTCCCACTACCTGCAGCATGTTTTAGAAGCAAAGATTTTGACGTTGTCAAGTCCTACGTCAACTCCACAGGCAAACCTCGAGCGAAACTACTAAAAAGTGAAGTCATAAAAGATCTTGCCGCACCTACTGTCGCTTCCTTCTCTTCACGGGGGCCCAATCGTATTTTATTTGAGATTATCAAGCCGGACATAACTGCCCCGGGGGTTGATATTTTGGCTGCATATTCACCTGTTGCTCCTGTCACAGAGAGCTTTGAAGACCGGAGGCACGTGAAATACAGTTTCCTATCTGGAACTTCCATGGCTTGCCCGCATGCTGCTGGTGTCGCTGCGTATATCAAAACATTCCACCCCAACTGGTCTCCAGCTGCCATCAAATCATCTATCATGACTACGGCTGGGCCTGCGAATGGCACTGACGATTCCTACACGCCCGGTGTATTTTCGTATGGTTCTGGGCAGATCGATAATCAATTCGGCGGAACTCTAAAAGCTTTAAATCCGGGCCTTGTGTATGAAACTTCTAAGGATGATTACATTAAGTTGCTCTGCTCGATCTTGGACGAGTCCAAAGTTCGACTTATTTCTGGAGACAAACGCCCTTGCCCTGCAAAGAATGACAAAATATCGGCCAAAGATCTCAACTACCCTTCAATGGCTGTTAACATTCTTGCAGAGACTTCGTTTTCGATTCAGTTTCAAAGAACAGTTAAAAATGTTGGCCATGCAAACTCCATTTACAAGGCAAAGACTGTTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

832

Amino Acids

89.91

Weight (kDa)

8.19

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 213 - 281 2.2e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 297 - 720 4.4e-42 Subtilase family
fn3_6 PF17766 791 - 829 1.5e-07 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1753
AccII CGCG 2 cut(s) 546, 1574
AciI CCGC 8 cut(s) 80, 158, 546, 1203, 1587, 1854, 2045, 2214
AclWI GGATC 5 cut(s) 229, 355, 664, 1316, 1329
AcoI YGGCCR 4 cut(s) 15, 497, 2364, 2455
AcsI RAATTY 6 cut(s) 253, 359, 403, 1006, 1110, 1513
AcvI CACGTG 1 cut(s) 2005
AcyI GRCGYC 1 cut(s) 9
AfaI GTAC 3 cut(s) 650, 1408, 1711
AfiI CCNNNNNNNGG 5 cut(s) 1121, 1402, 1403, 2144, 2389
AflIII ACRYGT 2 cut(s) 675, 1572
AgsI TTSAA 7 cut(s) 775, 856, 1287, 1991, 2388, 2435, 2492
AjiI CACGTC 1 cut(s) 676
AjnI CCWGG 1 cut(s) 555
AloI GAACNNNNNNTCC 4 cut(s) 662, 694, 1534, 1566
Alw21I GWGCWC 1 cut(s) 1039
Alw26I GTCTC 6 cut(s) 696, 844, 1287, 2100, 2319, 2405
AlwI GGATC 5 cut(s) 229, 355, 664, 1316, 1329
AlwNI CAGNNNCTG 1 cut(s) 1703
Ama87I CYCGRG 2 cut(s) 1812, 1933
ApaI GGGCCC 1 cut(s) 1890
ApeKI GCWGC 8 cut(s) 785, 1238, 1445, 1748, 1952, 2051, 2063, 2102
ApoI RAATTY 6 cut(s) 253, 359, 403, 1006, 1110, 1513
ArsI GACNNNNNNTTYG 2 cut(s) 2404, 2436
AspLEI GCGC 2 cut(s) 546, 1147
AspS9I GGNCC 6 cut(s) 962, 1394, 1886, 1887, 2135, 2239
AsuC2I CCSGG 5 cut(s) 309, 1934, 1935, 2169, 2238
AsuHPI GGTGA 3 cut(s) 328, 787, 1953
AsuII TTCGAA 1 cut(s) 740
AvaI CYCGRG 2 cut(s) 1812, 1933
AvaII GGWCC 1 cut(s) 962
AxyI CCTNAGG 1 cut(s) 666
BaeGI GKGCMC 1 cut(s) 1890
BaeI ACNNNNGTAYC 2 cut(s) 25, 58
BalI TGGCCA 3 cut(s) 17, 499, 2457
BamHI GGATCC 1 cut(s) 1321
BanII GRGCYC 2 cut(s) 1039, 1890
BarI GAAGNNNNNNTAC 2 cut(s) 721, 753
BbrPI CACGTG 1 cut(s) 2005
BbsI GAAGAC 1 cut(s) 1998
Bbv12I GWGCWC 1 cut(s) 1039
BbvCI CCTCAGC 2 cut(s) 228, 1057
BbvI GCAGC 8 cut(s) 772, 1225, 1457, 1760, 1939, 2038, 2050, 2089
BccI CCATC 5 cut(s) 35, 160, 286, 1256, 2114
BceAI ACGGC 3 cut(s) 599, 1408, 2145
BcgI CGANNNNNNTGC 2 cut(s) 2397, 2431
BciT130I CCWGG 1 cut(s) 557
BciVI GTATCC 1 cut(s) 1356
BcnI CCSGG 5 cut(s) 309, 1934, 1935, 2169, 2238
BcoDI GTCTC 6 cut(s) 696, 844, 1287, 2100, 2319, 2405
BfaI CTAG 2 cut(s) 1062, 1610
BfmI CTRYAG 5 cut(s) 1221, 1326, 1380, 1656, 1746
BfuAI ACCTGC 1 cut(s) 1753
BfuI GTATCC 1 cut(s) 1356
BglI GCCNNNNNGGC 1 cut(s) 77
BglII AGATCT 3 cut(s) 550, 1846, 2371
Bme1390I CCNGG 6 cut(s) 309, 557, 1934, 1935, 2169, 2238
Bme18I GGWCC 1 cut(s) 962
BmeT110I CYCGRG 2 cut(s) 1812, 1933
BmgBI CACGTC 1 cut(s) 676
BmgT120I GGNCC 6 cut(s) 962, 1394, 1886, 1887, 2135, 2239
BmiI GGNNCC 4 cut(s) 1054, 1323, 1887, 1888
BmrFI CCNGG 6 cut(s) 309, 557, 1934, 1935, 2169, 2238
BmrI ACTGGG 1 cut(s) 927
BmsI GCATC 3 cut(s) 262, 529, 1240
BmuI ACTGGG 1 cut(s) 927
BoxI GACNNNNGTC 1 cut(s) 1505
BpiI GAAGAC 1 cut(s) 1998
BpmI CTGGAG 4 cut(s) 143, 1373, 2082, 2343
Bpu10I CCTNAGC 2 cut(s) 228, 1057
Bpu14I TTCGAA 1 cut(s) 740
BpuEI CTTGAG 1 cut(s) 851
BpuMI CCSGG 5 cut(s) 309, 1934, 1935, 2169, 2238
Bsa29I ATCGAT 2 cut(s) 912, 2199
BsaAI YACGTR 1 cut(s) 2005
BsaBI GATNNNNATC 1 cut(s) 1320
BsaHI GRCGYC 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 2100
BsaJI CCNNGG 7 cut(s) 12, 413, 1427, 1932, 1933, 1934, 2034
BsaWI WCCGGW 2 cut(s) 1114, 1995
BsaXI ACNNNNNCTCC 2 cut(s) 1781, 1811
Bsc4I CCNNNNNNNGG 5 cut(s) 1121, 1402, 1403, 2144, 2389
Bse1I ACTGG 6 cut(s) 500, 500, 922, 1146, 1356, 2096
Bse21I CCTNAGG 1 cut(s) 666
Bse3DI GCAATG 2 cut(s) 21, 898
Bse8I GATNNNNATC 1 cut(s) 1320
BseBI CCWGG 1 cut(s) 557
BseCI ATCGAT 2 cut(s) 912, 2199
BseDI CCNNGG 7 cut(s) 12, 413, 1427, 1932, 1933, 1934, 2034
BseGI GGATG 6 cut(s) 27, 277, 381, 478, 676, 2269
BseJI GATNNNNATC 1 cut(s) 1320
BseLI CCNNNNNNNGG 5 cut(s) 1121, 1402, 1403, 2144, 2389
BseMI GCAATG 2 cut(s) 21, 898
BseMII CTCAG 3 cut(s) 219, 1071, 1388
BseNI ACTGG 6 cut(s) 500, 500, 922, 1146, 1356, 2096
BseRI GAGGAG 1 cut(s) 245
BseSI GKGCMC 1 cut(s) 1890
BseXI GCAGC 8 cut(s) 772, 1225, 1457, 1760, 1939, 2038, 2050, 2089
BseYI CCCAGC 1 cut(s) 2132
BsgI GTGCAG 1 cut(s) 1716
Bsh1236I CGCG 2 cut(s) 546, 1574
Bsh1285I CGRYCG 2 cut(s) 513, 802
BshVI ATCGAT 2 cut(s) 912, 2199
BsiEI CGRYCG 2 cut(s) 513, 802
BsiHKAI GWGCWC 1 cut(s) 1039
BsiHKCI CYCGRG 2 cut(s) 1812, 1933
BsiSI CCGG 7 cut(s) 308, 1115, 1919, 1934, 1996, 2169, 2237
BslFI GGGAC 2 cut(s) 1292, 1721
BslI CCNNNNNNNGG 5 cut(s) 1121, 1402, 1403, 2144, 2389
BsmAI GTCTC 6 cut(s) 696, 844, 1287, 2100, 2319, 2405
BsmFI GGGAC 2 cut(s) 1292, 1721
BsmI GAATGC 1 cut(s) 1589
Bso31I GGTCTC 1 cut(s) 2100
BsoBI CYCGRG 2 cut(s) 1812, 1933
Bsp119I TTCGAA 1 cut(s) 740
Bsp120I GGGCCC 1 cut(s) 1886
Bsp1286I GDGCHC 2 cut(s) 1039, 1890
Bsp19I CCATGG 4 cut(s) 12, 413, 1427, 2034
BspACI CCGC 8 cut(s) 80, 158, 546, 1203, 1587, 1854, 2045, 2214
BspCNI CTCAG 3 cut(s) 220, 1070, 1387
BspDI ATCGAT 2 cut(s) 912, 2199
BspFNI CGCG 2 cut(s) 546, 1574
BspHI TCATGA 1 cut(s) 2121
BspLI GGNNCC 4 cut(s) 1054, 1323, 1887, 1888
BspMAI CTGCAG 2 cut(s) 1384, 1750
BspMI ACCTGC 1 cut(s) 1753
BspPI GGATC 5 cut(s) 229, 355, 664, 1316, 1329
BspT104I TTCGAA 1 cut(s) 740
BspTNI GGTCTC 1 cut(s) 2100
BsrDI GCAATG 2 cut(s) 21, 898
BsrI ACTGG 6 cut(s) 500, 500, 922, 1146, 1356, 2096
BssECI CCNNGG 7 cut(s) 12, 413, 1427, 1932, 1933, 1934, 2034
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 4 cut(s) 12, 413, 1427, 2034
Bst2UI CCWGG 1 cut(s) 557
Bst6I CTCTTC 1 cut(s) 1882
BstACI GRCGYC 1 cut(s) 9
BstAPI GCANNNNNTGC 2 cut(s) 1337, 1694
BstBAI YACGTR 1 cut(s) 2005
BstBI TTCGAA 1 cut(s) 740
BstC8I GCNNGC 5 cut(s) 819, 2041, 2045, 2049, 2139
BstDEI CTNAG 8 cut(s) 46, 228, 666, 822, 1057, 1374, 1621, 2259
BstDSI CCRYGG 4 cut(s) 12, 413, 1427, 2034
BstEII GGTNACC 1 cut(s) 718
BstF5I GGATG 6 cut(s) 27, 277, 381, 478, 676, 2269
BstFNI CGCG 2 cut(s) 546, 1574
BstHHI GCGC 2 cut(s) 546, 1147
BstMAI GTCTC 6 cut(s) 696, 844, 1287, 2100, 2319, 2405
BstMCI CGRYCG 2 cut(s) 513, 802
BstMWI GCNNNNNNNGC 9 cut(s) 23, 77, 999, 1184, 1337, 1346, 1694, 2060, 2138
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 2 cut(s) 1755, 2051
BstPAI GACNNNNGTC 1 cut(s) 1505
BstPI GGTNACC 1 cut(s) 718
BstSCI CCNGG 6 cut(s) 307, 555, 1932, 1933, 2167, 2236
BstSFI CTRYAG 5 cut(s) 1221, 1326, 1380, 1656, 1746
BstSLI GKGCMC 1 cut(s) 1890
BstUI CGCG 2 cut(s) 546, 1574
BstV1I GCAGC 8 cut(s) 772, 1225, 1457, 1760, 1939, 2038, 2050, 2089
BstV2I GAAGAC 1 cut(s) 1998
BstX2I RGATCY 6 cut(s) 234, 347, 550, 1321, 1846, 2371
BstXI CCANNNNNNTGG 1 cut(s) 978
BstYI RGATCY 6 cut(s) 234, 347, 550, 1321, 1846, 2371
Bsu15I ATCGAT 2 cut(s) 912, 2199
Bsu36I CCTNAGG 1 cut(s) 666
BsuI GTATCC 1 cut(s) 1356
BsuTUI ATCGAT 2 cut(s) 912, 2199
BtgI CCRYGG 4 cut(s) 12, 413, 1427, 2034
BtgZI GCGATG 2 cut(s) 123, 963
BtrI CACGTC 1 cut(s) 676
BtsCI GGATG 6 cut(s) 27, 277, 381, 478, 676, 2269
BtsIMutI CAGTG 4 cut(s) 459, 507, 1349, 2148
BveI ACCTGC 1 cut(s) 1753
Cac8I GCNNGC 5 cut(s) 819, 2041, 2045, 2049, 2139
CaiI CAGNNNCTG 1 cut(s) 1703
CciI TCATGA 1 cut(s) 2121
CfoI GCGC 2 cut(s) 546, 1147
Cfr13I GGNCC 6 cut(s) 962, 1394, 1886, 1887, 2135, 2239
Cfr9I CCCGGG 1 cut(s) 1933
ClaI ATCGAT 2 cut(s) 912, 2199
CseI GACGC 3 cut(s) 17, 1563, 1580
Csp6I GTAC 3 cut(s) 649, 1407, 1710
CviQI GTAC 3 cut(s) 649, 1407, 1710
DdeI CTNAG 8 cut(s) 46, 228, 666, 822, 1057, 1374, 1621, 2259
DraI TTTAAA 3 cut(s) 364, 1536, 2232
EaeI YGGCCR 4 cut(s) 15, 497, 2364, 2455
Eam1104I CTCTTC 1 cut(s) 1882
EarI CTCTTC 1 cut(s) 1882
EciI GGCGGA 1 cut(s) 2229
Ecl136II GAGCTC 1 cut(s) 1037
Eco130I CCWWGG 4 cut(s) 12, 413, 1427, 2034
Eco24I GRGCYC 2 cut(s) 1039, 1890
Eco31I GGTCTC 1 cut(s) 2100
Eco32I GATATC 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 962
Eco53kI GAGCTC 1 cut(s) 1037
Eco72I CACGTG 1 cut(s) 2005
Eco81I CCTNAGG 1 cut(s) 666
Eco88I CYCGRG 2 cut(s) 1812, 1933
Eco91I GGTNACC 1 cut(s) 718
EcoICRI GAGCTC 1 cut(s) 1037
EcoO109I RGGNCCY 1 cut(s) 1886
EcoO65I GGTNACC 1 cut(s) 718
EcoRI GAATTC 2 cut(s) 1110, 1513
EcoRII CCWGG 1 cut(s) 555
EcoRV GATATC 1 cut(s) 450
EcoT14I CCWWGG 4 cut(s) 12, 413, 1427, 2034
EcoT38I GRGCYC 2 cut(s) 1039, 1890
ErhI CCWWGG 4 cut(s) 12, 413, 1427, 2034
FalI AAGNNNNNCTT 2 cut(s) 2298, 2330
FaqI GGGAC 2 cut(s) 1292, 1721
FauI CCCGC 2 cut(s) 73, 2052
FokI GGATG 6 cut(s) 14, 284, 368, 465, 683, 2276
FriOI GRGCYC 2 cut(s) 1039, 1890
FspBI CTAG 2 cut(s) 1062, 1610
GlaI GCGC 2 cut(s) 545, 1146
GsaI CCCAGC 1 cut(s) 2136
GsuI CTGGAG 4 cut(s) 143, 1373, 2082, 2343
HapII CCGG 7 cut(s) 308, 1115, 1919, 1934, 1996, 2169, 2237
HgaI GACGC 3 cut(s) 17, 1563, 1580
HhaI GCGC 2 cut(s) 546, 1147
Hin1I GRCGYC 1 cut(s) 9
Hin6I GCGC 2 cut(s) 544, 1145
HinP1I GCGC 2 cut(s) 544, 1145
HincII GTYRAC 3 cut(s) 1270, 1795, 2398
HindII GTYRAC 3 cut(s) 1270, 1795, 2398
HindIII AAGCTT 2 cut(s) 769, 2226
HpaI GTTAAC 1 cut(s) 2398
HpaII CCGG 7 cut(s) 308, 1115, 1919, 1934, 1996, 2169, 2237
HphI GGTGA 3 cut(s) 328, 787, 1953
Hpy166II GTNNAC 5 cut(s) 649, 1270, 1522, 1795, 2398
Hpy188I TCNGA 2 cut(s) 32, 937
Hpy8I GTNNAC 5 cut(s) 649, 1270, 1522, 1795, 2398
Hpy99I CGWCG 5 cut(s) 596, 620, 1394, 1579, 1680
HpyAV CCTTC 9 cut(s) 306, 485, 823, 947, 1491, 1556, 1595, 1882, 2394
HpyCH4IV ACGT 5 cut(s) 675, 1047, 1776, 1791, 2004
HpyF10VI GCNNNNNNNGC 9 cut(s) 23, 77, 999, 1184, 1337, 1346, 1694, 2060, 2138
HpyF3I CTNAG 8 cut(s) 46, 228, 666, 822, 1057, 1374, 1621, 2259
HpySE526I ACGT 5 cut(s) 675, 1047, 1776, 1791, 2004
Hsp92I GRCGYC 1 cut(s) 9
HspAI GCGC 2 cut(s) 544, 1145
KspAI GTTAAC 1 cut(s) 2398
LmnI GCTCC 4 cut(s) 973, 1034, 1685, 1975
Lsp1109I GCAGC 8 cut(s) 772, 1225, 1457, 1760, 1939, 2038, 2050, 2089
LweI GCATC 3 cut(s) 262, 529, 1240
MaeI CTAG 2 cut(s) 1062, 1610
MaeII ACGT 5 cut(s) 675, 1047, 1776, 1791, 2004
MaeIII GTNAC 6 cut(s) 41, 505, 718, 1463, 1699, 1975
MboII GAAGA 4 cut(s) 64, 96, 1869, 2003
MfeI CAATTG 4 cut(s) 298, 1254, 1287, 1299
MflI RGATCY 6 cut(s) 234, 347, 550, 1321, 1846, 2371
MhlI GDGCHC 2 cut(s) 1039, 1890
MlsI TGGCCA 3 cut(s) 17, 499, 2457
MluI ACGCGT 1 cut(s) 1572
MluNI TGGCCA 3 cut(s) 17, 499, 2457
MlyI GAGTC 3 cut(s) 143, 1289, 2309
MmeI TCCRAC 1 cut(s) 1663
Mox20I TGGCCA 3 cut(s) 17, 499, 2457
MscI TGGCCA 3 cut(s) 17, 499, 2457
MseI TTAA 8 cut(s) 363, 401, 1535, 1626, 2231, 2274, 2397, 2445
Msp20I TGGCCA 3 cut(s) 17, 499, 2457
MspA1I CMGCKG 1 cut(s) 2102
MspI CCGG 7 cut(s) 308, 1115, 1919, 1934, 1996, 2169, 2237
MspR9I CCNGG 6 cut(s) 309, 557, 1934, 1935, 2169, 2238
MunI CAATTG 4 cut(s) 298, 1254, 1287, 1299
Mva1269I GAATGC 1 cut(s) 1589
MvaI CCWGG 1 cut(s) 557
MvnI CGCG 2 cut(s) 546, 1574
MwoI GCNNNNNNNGC 9 cut(s) 23, 77, 999, 1184, 1337, 1346, 1694, 2060, 2138
NciI CCSGG 5 cut(s) 309, 1934, 1935, 2169, 2238
NcoI CCATGG 4 cut(s) 12, 413, 1427, 2034
NlaIV GGNNCC 4 cut(s) 1054, 1323, 1887, 1888
NmuCI GTSAC 3 cut(s) 41, 1463, 1975
NspI RCATGY 2 cut(s) 1755, 2051
NspV TTCGAA 1 cut(s) 740
PaeI GCATGC 1 cut(s) 2051
PaeR7I CTCGAG 1 cut(s) 1812
PagI TCATGA 1 cut(s) 2121
PcsI WCGNNNNNNNCGW 1 cut(s) 624
PctI GAATGC 1 cut(s) 1589
PfeI GAWTC 7 cut(s) 463, 742, 780, 932, 1432, 2156, 2425
PflFI GACNNNGTC 1 cut(s) 1778
Ple19I CGATCG 1 cut(s) 513
PleI GAGTC 3 cut(s) 142, 1289, 2308
PmaCI CACGTG 1 cut(s) 2005
PmlI CACGTG 1 cut(s) 2005
PpsI GAGTC 3 cut(s) 142, 1289, 2308
Ppu21I YACGTR 1 cut(s) 2005
PshAI GACNNNNGTC 1 cut(s) 1505
Psp124BI GAGCTC 1 cut(s) 1039
Psp6I CCWGG 1 cut(s) 555
PspCI CACGTG 1 cut(s) 2005
PspEI GGTNACC 1 cut(s) 718
PspFI CCCAGC 1 cut(s) 2132
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 4 cut(s) 1054, 1323, 1887, 1888
PspOMI GGGCCC 1 cut(s) 1886
PspPI GGNCC 6 cut(s) 962, 1394, 1886, 1887, 2135, 2239
PspXI VCTCGAGB 1 cut(s) 1812
PstI CTGCAG 2 cut(s) 1384, 1750
PstNI CAGNNNCTG 1 cut(s) 1703
PsuI RGATCY 6 cut(s) 234, 347, 550, 1321, 1846, 2371
PsyI GACNNNGTC 1 cut(s) 1778
PvuI CGATCG 1 cut(s) 513
PvuII CAGCTG 1 cut(s) 2102
RsaI GTAC 3 cut(s) 650, 1408, 1711
RsaNI GTAC 3 cut(s) 649, 1407, 1710
SacI GAGCTC 1 cut(s) 1039
SaqAI TTAA 8 cut(s) 363, 401, 1535, 1626, 2231, 2274, 2397, 2445
Sau96I GGNCC 6 cut(s) 962, 1394, 1886, 1887, 2135, 2239
SchI GAGTC 3 cut(s) 143, 1289, 2309
ScrFI CCNGG 6 cut(s) 309, 557, 1934, 1935, 2169, 2238
SduI GDGCHC 2 cut(s) 1039, 1890
SfaNI GCATC 3 cut(s) 262, 529, 1240
SfcI CTRYAG 5 cut(s) 1221, 1326, 1380, 1656, 1746
Sfr274I CTCGAG 1 cut(s) 1812
SfuI TTCGAA 1 cut(s) 740
SinI GGWCC 1 cut(s) 962
SlaI CTCGAG 1 cut(s) 1812
SmaI CCCGGG 1 cut(s) 1935
SmiI ATTTAAAT 1 cut(s) 1536
SmlI CTYRAG 2 cut(s) 866, 1812
SmoI CTYRAG 2 cut(s) 866, 1812
SphI GCATGC 1 cut(s) 2051
SsiI CCGC 8 cut(s) 80, 158, 546, 1203, 1587, 1854, 2045, 2214
SspMI CTAG 2 cut(s) 1062, 1610
SstI GAGCTC 1 cut(s) 1039
StyD4I CCNGG 6 cut(s) 307, 555, 1932, 1933, 2167, 2236
StyI CCWWGG 4 cut(s) 12, 413, 1427, 2034
SwaI ATTTAAAT 1 cut(s) 1536
TaiI ACGT 5 cut(s) 678, 1050, 1779, 1794, 2007
TaqII GACCGA 1 cut(s) 816
TatI WGTACW 1 cut(s) 648
TauI GCSGC 3 cut(s) 161, 1590, 1856
TfiI GAWTC 7 cut(s) 463, 742, 780, 932, 1432, 2156, 2425
Tru1I TTAA 8 cut(s) 363, 401, 1535, 1626, 2231, 2274, 2397, 2445
Tru9I TTAA 8 cut(s) 363, 401, 1535, 1626, 2231, 2274, 2397, 2445
TscAI CASTG 4 cut(s) 459, 507, 1356, 2155
TseFI GTSAC 3 cut(s) 41, 1463, 1975
TseI GCWGC 8 cut(s) 785, 1238, 1445, 1748, 1952, 2051, 2063, 2102
Tsp45I GTSAC 3 cut(s) 41, 1463, 1975
TspDTI ATGAA 9 cut(s) 370, 397, 467, 574, 842, 966, 1334, 1614, 2265
TspGWI ACGGA 4 cut(s) 792, 1152, 1164, 1509
TspMI CCCGGG 1 cut(s) 1933
TspRI CASTG 4 cut(s) 459, 507, 1356, 2155
Tth111I GACNNNGTC 1 cut(s) 1778
VpaK11BI GGWCC 1 cut(s) 962
XapI RAATTY 6 cut(s) 253, 359, 403, 1006, 1110, 1513
XceI RCATGY 2 cut(s) 1755, 2051
XcmI CCANNNNNNNNNTGG 1 cut(s) 420
XhoI CTCGAG 1 cut(s) 1812
XmaI CCCGGG 1 cut(s) 1933
XspI CTAG 2 cut(s) 1062, 1610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.