MD02G1289400.v1.1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000257
Physical Location & Seq
Forward (+)
34560968 .. 34565318
4351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1289400.v1.1.491

Sequence Viewer

Length: 2676 bp
ATGTCTTGCCCCCATGCTGCTGGCGCAGCTGCCTATATACAAGCATTCCACCCTGATTGGTCTGCAGCAGCCATCAAATCATCTCTTATGACTACAGGTGAATTTGCTCATGGTTCTGGACATATCAATCCTGTAAAAGCTATAAATCCGGGCCTTGTGTACGAAACATCTGAAGCTGATTACATAAGGGTGCTCTGCTCAACCTTGGATGAGGCCAAAGTTAGACTTGTATCGGGAGATAACAGCTCTTGTCCTATAGGCTCTGACAAAGGATCGGCGAAAGATCTCAACTATCCTTCTCTAGCTGCTTTTGTTTCCTTGAATGAGGAGAAGACTTTCACTGTGACCGTTGTTGGGAAAGATATACAAGATGCATCACATGTGTCTGCATCGCTGGTTTGGTCTGATGGAACACATACCGCTACAAGTCCAATTCTTGTACACAGTCATCGAAACAAAACAATGGCAAAGCACGGAGCTCTATTGGTTTCTTGTGCTTTCTCCACTATTCTCATACTCTCTGTGAGCTTACCGTGTAGAGCCATTGATCAGGAAGATAGAAAGACATACATTGTCTACTTGGGATCACTTCCTAAGGATGAGTTATTCTCGCCGTTGTCTCACCACATTGACATTCTCGAAAGCGTCGTGGAGAGCAGCTCTGCTTCAAATCTATTGGTAAGAACCTACAAAAGGAGCTTCAACGGATTTGCTGCCAAGCTCACTGAGCAAGAAAGAGAAAGGCTTGCTAACATGAAGGAAGTTGTCTCTGTGTTTCCAAGCACGAGTTACAAACCCCAAACAACAAGATCTTGGGACTTTATCGGGTTCAATGAGAATATCAAACGAAACCGTACTGTCGAGAGTGATGTCATTATTGGTGTGATCGACAGTGGAATCTGGCCTGAATCGGAGAGCTTTAAAGACAAAGGTTTTGGTCCTGCTCCCAAGAAGTGGAAAGGTGCTTGTCAAGGAGGCAAAAATTTCACTTGCAACAACAAGATCATTGGAGCTCGATTTTACACTAATTCCACGGCCAGAGATGATGTTGGTCATGGAACTCATACTGCCTCAACTGCAGCAGGGAACCCCGTAAAGGGTGTGAGCTTTTATGGAATAGCACAAGGCACCGCAAGAGGAGGCGTTCCCTTAGCTAGAATTGCTACATACAAAGTTTGCGGTTCTAACGGGTGCAATACAGATGGGCTCTTGGCTGCTTTTGATGACGCCATCGCCGATGGAGTTGACATCATTACAATTTCAGTTGGAACCGATTTTACACCTCCTTTCGAGCAGGATCCTATTGCAATCGGTGCTTTTCATGCAATGGAGAAAGGGATACTTACCTTGCATTCTGCAGGCAATAACGGCCCTCAAGAGGGTTCTGTATCAAGTGTAGCACCATGGATTCTTACAGTTGCAGCAAGTAGCACAGATCGTCGGATAATTGACAAGGCTGTTCTTGGAAATGGAAGGACGCTTGTCGGAAATTCAGTGAATGCTTTCAAATTAAATGGAACAAGTTTTCCGTTGATAGAAGGAAAAAATGCGTCAAGTCAATGCTCGGATTGTAAAGCAGCCTGCCTAGACCCTGAATTAGTTAAGGGAAAGATTGTGGTATGTCTTCGTGAGGGTGGCAGTATTGTGGCTCATCAAGTCGGTGCAATAGGTGCAATTACAATCAGTTCTGCACCTGATGTTTCTTTCATTGTCCCACTACCCGCAGCAACTATTAACGTTGAAGATTATTTAATTCTCGTTTCCTACCTCAACTCCACAGCCAAACCTCGGGCGAACATACTGAAAAGTGAAGCCATAAAAGATCTTGCTGCACCTATTGTTGCTTCCTTCTCTTCGCGGGGGCCTAATTTTATTCTACCTGAAATTATCAAGCCAGATATAACAGCCCCGGGGGTTGATATTTTGGCTGCATTTTCACCTGTCGTTGGTGTCACAGGTTCCCCTAAAGACAAAAGGCATGTAAAATACAGCTTACTATCTGGAACCTCCATGGCTTGCCCCCATGCTGCTGGTGCTGCTGCATATATAAGAACATTCCACCCAGATTGGTCTCCGGCAGCCATCAAATCATCTCTTATGACTACAGCTTGGCTCATGAATAATACTGACTATTATTATCCTGGTGAATTTGCTTATGGTTCTGGACATATCAATCCTCTCAAGGCTGTTGATCCTGGGCTTGTGTTCGAAACTTCTAGAGGCGATTACATAAAGTTGCTCTGCTCAATCTTGGATGAGGCCAAAGTTATACTTATATCAGGAGATAACAGCACCTGTCCTACAGGCTCTGAGAAAGGATCATCAAAGGATCTCAATTATCCTTCAATAGCTGCTAATGTTACACCAATGGAATCATTTGCGATTAAATTCAGCAGAACAGTTAAAAATGTTGGCCTCACAAACTCTACTTTCAAGGCTAAAATCCTCACAAATTCGAAGGCAAGATTCCTACCAACTACGGAAGTTGACATCAAAGTGACTCCTGAAGTTCTTTCGTTCAAGTCCTTGAATGAGGAGAAGGCTTTTACTGTGACCGTTGTTGGAAAAGATTTTCCGAGCGGATCACATCTGTCTGCGTCACTGGTTTGGTCCGATGGAACTCATACTGTTAGAAGTCCAGTTATTGTACGCAGTGTACAGAGTGTCAGAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

892

Amino Acids

94.72

Weight (kDa)

7.82

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 189 - 265 9.6e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 289 - 709 3.8e-43 Subtilase family
fn3_6 PF17766 777 - 883 1.1e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1127
AccB7I CCANNNNNTGG 1 cut(s) 954
AccBSI CCGCTC 1 cut(s) 2580
AccI GTMKAC 1 cut(s) 576
AccII CGCG 1 cut(s) 1858
AciI CCGC 6 cut(s) 420, 1131, 1179, 1722, 1858, 2580
AclI AACGTT 1 cut(s) 1737
AclWI GGATC 8 cut(s) 280, 592, 1292, 1305, 2186, 2326, 2337, 2590
AcoI YGGCCR 1 cut(s) 1035
AcsI RAATTY 7 cut(s) 101, 982, 1489, 2147, 2387, 2452, 2670
AcuI CTGAAG 2 cut(s) 192, 2526
AcyI GRCGYC 1 cut(s) 1227
AfaI GTAC 5 cut(s) 161, 441, 856, 2649, 2658
AfiI CCNNNNNNNGG 9 cut(s) 354, 693, 954, 1096, 1097, 1378, 1379, 1788, 1946
AflIII ACRYGT 1 cut(s) 379
AjnI CCWGG 2 cut(s) 2140, 2194
AloI GAACNNNNNNTCC 2 cut(s) 1510, 1542
Alw21I GWGCWC 3 cut(s) 195, 481, 1015
Alw26I GTCTC 3 cut(s) 624, 772, 2076
AlwI GGATC 8 cut(s) 280, 592, 1292, 1305, 2186, 2326, 2337, 2590
AlwNI CAGNNNCTG 2 cut(s) 2295, 2309
Ama87I CYCGRG 2 cut(s) 1788, 1909
AoxI GGCC 8 cut(s) 151, 213, 902, 1035, 1369, 1862, 2259, 2413
ApoI RAATTY 7 cut(s) 101, 982, 1489, 2147, 2387, 2452, 2670
ArsI GACNNNNNNTTYG 2 cut(s) 917, 949
Asp700I GAANNNNTTC 2 cut(s) 335, 1502
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 5 cut(s) 151, 938, 1370, 1862, 2610
AsuC2I CCSGG 3 cut(s) 150, 1910, 1911
AsuHPI GGTGA 4 cut(s) 110, 614, 1929, 2156
AsuII TTCGAA 2 cut(s) 2208, 2456
AvaI CYCGRG 2 cut(s) 1788, 1909
AvaII GGWCC 2 cut(s) 938, 2610
AxyI CCTNAGG 1 cut(s) 594
BamHI GGATCC 1 cut(s) 1297
BanI GGYRCC 1 cut(s) 1127
BanII GRGCYC 3 cut(s) 481, 1015, 1209
BauI CACGAG 1 cut(s) 784
BbsI GAAGAC 2 cut(s) 338, 1616
Bbv12I GWGCWC 3 cut(s) 195, 481, 1015
BccI CCATC 7 cut(s) 80, 401, 1196, 1232, 1238, 2090, 2609
BceAI ACGGC 3 cut(s) 598, 1050, 1384
BciT130I CCWGG 2 cut(s) 2142, 2196
BciVI GTATCC 1 cut(s) 1332
BclI TGATCA 1 cut(s) 547
BcnI CCSGG 3 cut(s) 150, 1910, 1911
BcoDI GTCTC 3 cut(s) 624, 772, 2076
BfaI CTAG 4 cut(s) 302, 1155, 1586, 2217
BfmI CTRYAG 7 cut(s) 63, 93, 255, 1077, 1356, 2103, 2301
BfuI GTATCC 1 cut(s) 1332
BglII AGATCT 3 cut(s) 283, 809, 1822
Bme1390I CCNGG 5 cut(s) 150, 1910, 1911, 2142, 2196
Bme18I GGWCC 2 cut(s) 938, 2610
BmeT110I CYCGRG 2 cut(s) 1788, 1909
BmgT120I GGNCC 5 cut(s) 151, 938, 1370, 1862, 2610
BmiI GGNNCC 7 cut(s) 1088, 1129, 1270, 1299, 1863, 1960, 2005
BmrFI CCNGG 5 cut(s) 150, 1910, 1911, 2142, 2196
BmsI GCATC 3 cut(s) 361, 383, 398
BoxI GACNNNNGTC 1 cut(s) 1481
BpiI GAAGAC 2 cut(s) 338, 1616
BplI GAGNNNNNCTC 4 cut(s) 593, 625, 644, 676
Bpu10I CCTNAGC 1 cut(s) 1150
Bpu14I TTCGAA 2 cut(s) 2208, 2456
BpuEI CTTGAG 2 cut(s) 1359, 2165
BpuMI CCSGG 3 cut(s) 150, 1910, 1911
BsaHI GRCGYC 1 cut(s) 1227
BsaI GGTCTC 1 cut(s) 2076
BsaJI CCNNGG 9 cut(s) 204, 1032, 1403, 1787, 1908, 1909, 1910, 2010, 2195
BsaXI ACNNNNNCTCC 2 cut(s) 1757, 1787
Bsc4I CCNNNNNNNGG 9 cut(s) 354, 693, 954, 1096, 1097, 1378, 1379, 1788, 1946
Bse1I ACTGG 2 cut(s) 2607, 2639
Bse21I CCTNAGG 1 cut(s) 594
Bse3DI GCAATG 1 cut(s) 1332
BseBI CCWGG 2 cut(s) 2142, 2196
BseDI CCNNGG 9 cut(s) 204, 1032, 1403, 1787, 1908, 1909, 1910, 2010, 2195
BseGI GGATG 3 cut(s) 214, 604, 2260
BseLI CCNNNNNNNGG 9 cut(s) 354, 693, 954, 1096, 1097, 1378, 1379, 1788, 1946
BseMI GCAATG 1 cut(s) 1332
BseMII CTCAG 2 cut(s) 717, 2301
BseNI ACTGG 2 cut(s) 2607, 2639
BseRI GAGGAG 3 cut(s) 341, 1152, 2549
BsgI GTGCAG 2 cut(s) 1674, 1815
Bsh1236I CGCG 1 cut(s) 1858
BshFI GGCC 8 cut(s) 153, 215, 904, 1037, 1371, 1864, 2261, 2415
BshNI GGYRCC 1 cut(s) 1127
BsiHKAI GWGCWC 3 cut(s) 195, 481, 1015
BsiHKCI CYCGRG 2 cut(s) 1788, 1909
BsiSI CCGG 3 cut(s) 149, 1910, 2075
BslFI GGGAC 2 cut(s) 830, 1697
BslI CCNNNNNNNGG 9 cut(s) 354, 693, 954, 1096, 1097, 1378, 1379, 1788, 1946
BsmAI GTCTC 3 cut(s) 624, 772, 2076
BsmFI GGGAC 2 cut(s) 830, 1697
BsmI GAATGC 3 cut(s) 44, 1351, 1504
BsnI GGCC 8 cut(s) 153, 215, 904, 1037, 1371, 1864, 2261, 2415
Bso31I GGTCTC 1 cut(s) 2076
BsoBI CYCGRG 2 cut(s) 1788, 1909
Bsp119I TTCGAA 2 cut(s) 2208, 2456
Bsp1286I GDGCHC 4 cut(s) 195, 481, 1015, 1209
Bsp1407I TGTACA 2 cut(s) 439, 2656
Bsp19I CCATGG 2 cut(s) 1403, 2010
BspACI CCGC 6 cut(s) 420, 1131, 1179, 1722, 1858, 2580
BspANI GGCC 8 cut(s) 153, 215, 904, 1037, 1371, 1864, 2261, 2415
BspCNI CTCAG 2 cut(s) 718, 2302
BspFNI CGCG 1 cut(s) 1858
BspHI TCATGA 1 cut(s) 2115
BspLI GGNNCC 7 cut(s) 1088, 1129, 1270, 1299, 1863, 1960, 2005
BspMAI CTGCAG 3 cut(s) 67, 1081, 1360
BspPI GGATC 8 cut(s) 280, 592, 1292, 1305, 2186, 2326, 2337, 2590
BspT104I TTCGAA 2 cut(s) 2208, 2456
BspT107I GGYRCC 1 cut(s) 1127
BspTNI GGTCTC 1 cut(s) 2076
BsrBI CCGCTC 1 cut(s) 2580
BsrDI GCAATG 1 cut(s) 1332
BsrGI TGTACA 2 cut(s) 439, 2656
BsrI ACTGG 2 cut(s) 2607, 2639
BssECI CCNNGG 9 cut(s) 204, 1032, 1403, 1787, 1908, 1909, 1910, 2010, 2195
BssNI GRCGYC 1 cut(s) 1227
BssSI CACGAG 1 cut(s) 784
BssT1I CCWWGG 3 cut(s) 204, 1403, 2010
Bst2BI CACGAG 1 cut(s) 784
Bst2UI CCWGG 2 cut(s) 2142, 2196
Bst6I CTCTTC 1 cut(s) 1858
BstACI GRCGYC 1 cut(s) 1227
BstAPI GCANNNNNTGC 2 cut(s) 1313, 1670
BstAUI TGTACA 2 cut(s) 439, 2656
BstBI TTCGAA 2 cut(s) 2208, 2456
BstC8I GCNNGC 5 cut(s) 22, 747, 1360, 1582, 2017
BstDEI CTNAG 4 cut(s) 594, 726, 1150, 2310
BstDSI CCRYGG 3 cut(s) 1032, 1403, 2010
BstENI CCTNNNNNAGG 1 cut(s) 691
BstF5I GGATG 3 cut(s) 214, 604, 2260
BstFNI CGCG 1 cut(s) 1858
BstHHI GCGC 1 cut(s) 26
BstMAI GTCTC 3 cut(s) 624, 772, 2076
BstNI CCWGG 2 cut(s) 2142, 2196
BstNSI RCATGY 2 cut(s) 383, 1982
BstPAI GACNNNNGTC 1 cut(s) 1481
BstSCI CCNGG 5 cut(s) 148, 1908, 1909, 2140, 2194
BstSFI CTRYAG 7 cut(s) 63, 93, 255, 1077, 1356, 2103, 2301
BstUI CGCG 1 cut(s) 1858
BstV2I GAAGAC 2 cut(s) 338, 1616
BstX2I RGATCY 5 cut(s) 283, 809, 1297, 1822, 2329
BstXI CCANNNNNNTGG 2 cut(s) 20, 2030
BstYI RGATCY 5 cut(s) 283, 809, 1297, 1822, 2329
Bsu36I CCTNAGG 1 cut(s) 594
BsuI GTATCC 1 cut(s) 1332
BsuRI GGCC 8 cut(s) 153, 215, 904, 1037, 1371, 1864, 2261, 2415
BtgI CCRYGG 3 cut(s) 1032, 1403, 2010
BtgZI GCGATG 2 cut(s) 375, 1216
BtsCI GGATG 3 cut(s) 214, 604, 2260
BtsI GCAGTG 1 cut(s) 2659
BtsIMutI CAGTG 6 cut(s) 339, 723, 898, 1500, 2600, 2659
Cac8I GCNNGC 5 cut(s) 22, 747, 1360, 1582, 2017
CaiI CAGNNNCTG 2 cut(s) 2295, 2309
CciI TCATGA 1 cut(s) 2115
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 5 cut(s) 151, 938, 1370, 1862, 2610
Cfr9I CCCGGG 1 cut(s) 1909
CseI GACGC 5 cut(s) 634, 1235, 1486, 1539, 2586
Csp6I GTAC 5 cut(s) 160, 440, 855, 2648, 2657
CspCI CAANNNNNGTGG 4 cut(s) 38, 73, 2048, 2083
CviQI GTAC 5 cut(s) 160, 440, 855, 2648, 2657
DdeI CTNAG 4 cut(s) 594, 726, 1150, 2310
DraI TTTAAA 1 cut(s) 922
EaeI YGGCCR 1 cut(s) 1035
Eam1104I CTCTTC 1 cut(s) 1858
EarI CTCTTC 1 cut(s) 1858
Ecl136II GAGCTC 2 cut(s) 479, 1013
Eco130I CCWWGG 3 cut(s) 204, 1403, 2010
Eco24I GRGCYC 3 cut(s) 481, 1015, 1209
Eco31I GGTCTC 1 cut(s) 2076
Eco47I GGWCC 2 cut(s) 938, 2610
Eco53kI GAGCTC 2 cut(s) 479, 1013
Eco57I CTGAAG 2 cut(s) 192, 2526
Eco81I CCTNAGG 1 cut(s) 594
Eco88I CYCGRG 2 cut(s) 1788, 1909
EcoICRI GAGCTC 2 cut(s) 479, 1013
EcoNI CCTNNNNNAGG 1 cut(s) 691
EcoO109I RGGNCCY 1 cut(s) 1862
EcoRII CCWGG 2 cut(s) 2140, 2194
EcoT14I CCWWGG 3 cut(s) 204, 1403, 2010
EcoT22I ATGCAT 1 cut(s) 376
EcoT38I GRGCYC 3 cut(s) 481, 1015, 1209
ErhI CCWWGG 3 cut(s) 204, 1403, 2010
FalI AAGNNNNNCTT 6 cut(s) 210, 242, 1326, 1358, 2256, 2288
FaqI GGGAC 2 cut(s) 830, 1697
FauI CCCGC 2 cut(s) 1729, 1851
FbaI TGATCA 1 cut(s) 547
FblI GTMKAC 1 cut(s) 576
FokI GGATG 3 cut(s) 221, 611, 2267
FriOI GRGCYC 3 cut(s) 481, 1015, 1209
FspBI CTAG 4 cut(s) 302, 1155, 1586, 2217
GlaI GCGC 1 cut(s) 25
HaeIII GGCC 8 cut(s) 153, 215, 904, 1037, 1371, 1864, 2261, 2415
HapII CCGG 3 cut(s) 149, 1910, 2075
HgaI GACGC 5 cut(s) 634, 1235, 1486, 1539, 2586
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 1227
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 2 cut(s) 1246, 2488
HindII GTYRAC 2 cut(s) 1246, 2488
HinfI GANTC 6 cut(s) 897, 908, 1408, 2372, 2466, 2500
HpaII CCGG 3 cut(s) 149, 1910, 2075
HphI GGTGA 4 cut(s) 110, 614, 1929, 2156
Hpy166II GTNNAC 6 cut(s) 160, 442, 577, 1246, 2488, 2657
Hpy8I GTNNAC 6 cut(s) 160, 442, 577, 1246, 2488, 2657
Hpy99I CGWCG 2 cut(s) 650, 1443
HpyAV CCTTC 8 cut(s) 306, 751, 1467, 1532, 1858, 2352, 2452, 2533
HpyCH4IV ACGT 1 cut(s) 1737
HpyF3I CTNAG 4 cut(s) 594, 726, 1150, 2310
HpySE526I ACGT 1 cut(s) 1737
Hsp92I GRCGYC 1 cut(s) 1227
HspAI GCGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 547
LmnI GCTCC 4 cut(s) 476, 696, 949, 1010
LweI GCATC 3 cut(s) 361, 383, 398
MaeI CTAG 4 cut(s) 302, 1155, 1586, 2217
MaeII ACGT 1 cut(s) 1737
MaeIII GTNAC 7 cut(s) 343, 788, 1951, 2359, 2497, 2551, 2598
MbiI CCGCTC 1 cut(s) 2580
MboII GAAGA 5 cut(s) 343, 566, 1616, 1754, 1845
MflI RGATCY 5 cut(s) 283, 809, 1297, 1822, 2329
MhlI GDGCHC 4 cut(s) 195, 481, 1015, 1209
MlyI GAGTC 1 cut(s) 2494
MmeI TCCRAC 4 cut(s) 1246, 1421, 1465, 2542
Mph1103I ATGCAT 1 cut(s) 376
MroXI GAANNNNTTC 2 cut(s) 335, 1502
MseI TTAA 8 cut(s) 921, 1511, 1602, 1734, 1751, 2385, 2403, 2674
MslI CAYNNNNRTG 2 cut(s) 188, 2495
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 3 cut(s) 149, 1910, 2075
MspR9I CCNGG 5 cut(s) 150, 1910, 1911, 2142, 2196
Mva1269I GAATGC 3 cut(s) 44, 1351, 1504
MvaI CCWGG 2 cut(s) 2142, 2196
MvnI CGCG 1 cut(s) 1858
NciI CCSGG 3 cut(s) 150, 1910, 1911
NcoI CCATGG 2 cut(s) 1403, 2010
NlaIV GGNNCC 7 cut(s) 1088, 1129, 1270, 1299, 1863, 1960, 2005
NmuCI GTSAC 5 cut(s) 343, 1951, 2497, 2551, 2598
NsiI ATGCAT 1 cut(s) 376
NspI RCATGY 2 cut(s) 383, 1982
NspV TTCGAA 2 cut(s) 2208, 2456
PagI TCATGA 1 cut(s) 2115
PciI ACATGT 1 cut(s) 379
PcsI WCGNNNNNNNCGW 2 cut(s) 645, 1233
PctI GAATGC 3 cut(s) 44, 1351, 1504
PdmI GAANNNNTTC 2 cut(s) 335, 1502
PfeI GAWTC 5 cut(s) 897, 908, 1408, 2372, 2466
PflMI CCANNNNNTGG 1 cut(s) 954
PleI GAGTC 1 cut(s) 2494
PpsI GAGTC 1 cut(s) 2494
PscI ACATGT 1 cut(s) 379
PshAI GACNNNNGTC 1 cut(s) 1481
Psp124BI GAGCTC 2 cut(s) 481, 1015
Psp1406I AACGTT 1 cut(s) 1737
Psp6I CCWGG 2 cut(s) 2140, 2194
PspGI CCWGG 2 cut(s) 2140, 2194
PspN4I GGNNCC 7 cut(s) 1088, 1129, 1270, 1299, 1863, 1960, 2005
PspPI GGNCC 5 cut(s) 151, 938, 1370, 1862, 2610
PsrI GAACNNNNNNTAC 2 cut(s) 1261, 1293
PstI CTGCAG 3 cut(s) 67, 1081, 1360
PstNI CAGNNNCTG 2 cut(s) 2295, 2309
PsuI RGATCY 5 cut(s) 283, 809, 1297, 1822, 2329
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 5 cut(s) 161, 441, 856, 2649, 2658
RsaNI GTAC 5 cut(s) 160, 440, 855, 2648, 2657
RseI CAYNNNNRTG 2 cut(s) 188, 2495
SacI GAGCTC 2 cut(s) 481, 1015
SaqAI TTAA 8 cut(s) 921, 1511, 1602, 1734, 1751, 2385, 2403, 2674
Sau96I GGNCC 5 cut(s) 151, 938, 1370, 1862, 2610
SchI GAGTC 1 cut(s) 2494
ScrFI CCNGG 5 cut(s) 150, 1910, 1911, 2142, 2196
SduI GDGCHC 4 cut(s) 195, 481, 1015, 1209
SfaNI GCATC 3 cut(s) 361, 383, 398
SfcI CTRYAG 7 cut(s) 63, 93, 255, 1077, 1356, 2103, 2301
SfuI TTCGAA 2 cut(s) 2208, 2456
SinI GGWCC 2 cut(s) 938, 2610
SmaI CCCGGG 1 cut(s) 1911
SmiMI CAYNNNNRTG 2 cut(s) 188, 2495
SmlI CTYRAG 2 cut(s) 1374, 2180
SmoI CTYRAG 2 cut(s) 1374, 2180
SsiI CCGC 6 cut(s) 420, 1131, 1179, 1722, 1858, 2580
SspMI CTAG 4 cut(s) 302, 1155, 1586, 2217
SstI GAGCTC 2 cut(s) 481, 1015
StyD4I CCNGG 5 cut(s) 148, 1908, 1909, 2140, 2194
StyI CCWWGG 3 cut(s) 204, 1403, 2010
TaiI ACGT 1 cut(s) 1740
TaqI TCGA 8 cut(s) 451, 639, 861, 888, 1015, 1290, 2208, 2456
TatI WGTACW 2 cut(s) 439, 2656
TfiI GAWTC 5 cut(s) 897, 908, 1408, 2372, 2466
Tru1I TTAA 8 cut(s) 921, 1511, 1602, 1734, 1751, 2385, 2403, 2674
Tru9I TTAA 8 cut(s) 921, 1511, 1602, 1734, 1751, 2385, 2403, 2674
TscAI CASTG 6 cut(s) 346, 730, 898, 1500, 2607, 2659
TseFI GTSAC 5 cut(s) 343, 1951, 2497, 2551, 2598
Tsp45I GTSAC 5 cut(s) 343, 1951, 2497, 2551, 2598
TspDTI ATGAA 4 cut(s) 770, 1310, 1696, 2132
TspGWI ACGGA 4 cut(s) 489, 720, 1518, 2495
TspMI CCCGGG 1 cut(s) 1909
TspRI CASTG 6 cut(s) 346, 730, 898, 1500, 2607, 2659
Van91I CCANNNNNTGG 1 cut(s) 954
VpaK11BI GGWCC 2 cut(s) 938, 2610
XagI CCTNNNNNAGG 1 cut(s) 691
XapI RAATTY 7 cut(s) 101, 982, 1489, 2147, 2387, 2452, 2670
XbaI TCTAGA 1 cut(s) 2216
XceI RCATGY 2 cut(s) 383, 1982
XmaI CCCGGG 1 cut(s) 1909
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 2 cut(s) 335, 1502
XspI CTAG 4 cut(s) 302, 1155, 1586, 2217
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.