MD07G1037700.v1.1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000257
Physical Location & Seq
Reverse (-)
3089964 .. 3090506
543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1037700.v1.1.491

Sequence Viewer

Length: 543 bp
ATGTTTTGGTGCGTATTGTGCACAGGCTGCCTAAAAAGTAGTTTAGTTAAGGAAAAGATTGTGGTGTGTGATTCATACAATGGAGATAGTGAAGCTCGTCGAGCCGGTGCACAAGGTGCTATTTTAACCAATTGGAATAACGAAGCTTCTATTGTCCCACTACCTGCAACAACTTTAAGTAGTGAAGATAATTATGCGGTCAAGTCCTACATCAACTCAACAACAGCCGCTCGAGCCAACATATTAAAAACCGAAGCAATAAAAGATCACGCTGCACCTGTTGTTGCTTCCTCTTCTTCGTTGGGGCCAAATTTAATTTTACCTGAAATTATCAAGCCAGATATAACTGCCCCAGGGGTGAATATCTTGGCTGCATTTTCGCCTGTCGCTCGTGTAACAGATACCCCTGAAGACAAAAGGCATGCAAAATACAGCTTTCTGTCTGGAACCTCCATGTCTTGCCCCCACGCGCTGGTTCCGCAGCATATGTTCGAACATTCCACCCTGATTGGTCTCCTCCGGCCATCAAATAATCTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

181

Amino Acids

19.25

Weight (kDa)

6.95

Isoelectric Point (pI)

57.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 21 - 157 2.4e-09 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 172
AccB7I CCANNNNNTGG 1 cut(s) 472
AccBSI CCGCTC 1 cut(s) 230
AccII CGCG 1 cut(s) 470
AciI CCGC 3 cut(s) 197, 228, 479
AcoI YGGCCR 1 cut(s) 521
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 429
AdeI CACNNNGTG 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 472
AjnI CCWGG 1 cut(s) 352
AluBI AGCT 3 cut(s) 95, 146, 435
AluI AGCT 3 cut(s) 95, 146, 435
Alw21I GWGCWC 2 cut(s) 23, 112
Alw26I GTCTC 1 cut(s) 518
Alw44I GTGCAC 2 cut(s) 19, 108
Ama87I CYCGRG 1 cut(s) 231
AoxI GGCC 2 cut(s) 305, 521
ApaLI GTGCAC 2 cut(s) 19, 108
ApeKI GCWGC 4 cut(s) 27, 272, 371, 481
ApoI RAATTY 1 cut(s) 310
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 370
AsuII TTCGAA 1 cut(s) 492
AvaI CYCGRG 1 cut(s) 231
BaeGI GKGCMC 2 cut(s) 23, 112
BauI CACGAG 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 2 cut(s) 23, 112
BbvI GCAGC 4 cut(s) 14, 259, 358, 493
BccI CCATC 1 cut(s) 532
BciT130I CCWGG 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 518
BfuAI ACCTGC 1 cut(s) 172
BisI GCNGC 5 cut(s) 28, 228, 273, 372, 482
BlsI GCNGC 5 cut(s) 29, 229, 274, 373, 483
Bme1390I CCNGG 1 cut(s) 354
BmeT110I CYCGRG 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 3 cut(s) 306, 448, 477
BmrFI CCNGG 1 cut(s) 354
BpiI GAAGAC 1 cut(s) 417
Bpu14I TTCGAA 1 cut(s) 492
BsaI GGTCTC 1 cut(s) 518
BsaJI CCNNGG 2 cut(s) 352, 353
Bsc4I CCNNNNNNNGG 1 cut(s) 472
Bse118I RCCGGY 1 cut(s) 104
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 2 cut(s) 352, 353
BseLI CCNNNNNNNGG 1 cut(s) 472
BseRI GAGGAG 1 cut(s) 506
BseSI GKGCMC 2 cut(s) 23, 112
BseXI GCAGC 4 cut(s) 14, 259, 358, 493
BsgI GTGCAG 1 cut(s) 258
Bsh1236I CGCG 1 cut(s) 470
BshFI GGCC 2 cut(s) 307, 523
BsiHKAI GWGCWC 2 cut(s) 23, 112
BsiHKCI CYCGRG 1 cut(s) 231
BsiSI CCGG 2 cut(s) 105, 520
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 1 cut(s) 472
BsmAI GTCTC 1 cut(s) 518
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 2 cut(s) 307, 523
Bso31I GGTCTC 1 cut(s) 518
BsoBI CYCGRG 1 cut(s) 231
Bsp119I TTCGAA 1 cut(s) 492
Bsp1286I GDGCHC 2 cut(s) 23, 112
Bsp143I GATC 1 cut(s) 265
BspACI CCGC 3 cut(s) 197, 228, 479
BspANI GGCC 2 cut(s) 307, 523
BspFNI CGCG 1 cut(s) 470
BspLI GGNNCC 3 cut(s) 306, 448, 477
BspMI ACCTGC 1 cut(s) 172
BspT104I TTCGAA 1 cut(s) 492
BspTNI GGTCTC 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 230
BsrFI RCCGGY 1 cut(s) 104
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 2 cut(s) 352, 353
BssMI GATC 1 cut(s) 265
BssSI CACGAG 1 cut(s) 390
Bst2BI CACGAG 1 cut(s) 390
Bst2UI CCWGG 1 cut(s) 354
Bst6I CTCTTC 1 cut(s) 298
BstAPI GCANNNNNTGC 2 cut(s) 27, 116
BstBI TTCGAA 1 cut(s) 492
BstC8I GCNNGC 1 cut(s) 423
BstFNI CGCG 1 cut(s) 470
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 1 cut(s) 518
BstMBI GATC 1 cut(s) 265
BstMWI GCNNNNNNNGC 6 cut(s) 18, 27, 101, 116, 233, 478
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 352
BstSLI GKGCMC 2 cut(s) 23, 112
BstUI CGCG 1 cut(s) 470
BstV1I GCAGC 4 cut(s) 14, 259, 358, 493
BstV2I GAAGAC 1 cut(s) 417
BsuRI GGCC 2 cut(s) 307, 523
BveI ACCTGC 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 423
CfoI GCGC 1 cut(s) 472
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 305
CspCI CAANNNNNGTGG 2 cut(s) 490, 525
CviAII CATG 2 cut(s) 422, 454
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
DraIII CACNNNGTG 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 521
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
Eco31I GGTCTC 1 cut(s) 518
Eco57I CTGAAG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 352
FaeI CATG 2 cut(s) 425, 457
FaiI YATR 9 cut(s) 76, 195, 242, 344, 423, 455, 486, 488, 541
FaqI GGGAC 1 cut(s) 140
FatI CATG 2 cut(s) 421, 453
FauNDI CATATG 1 cut(s) 486
Fnu4HI GCNGC 5 cut(s) 28, 228, 273, 372, 482
Fsp4HI GCNGC 5 cut(s) 28, 228, 273, 372, 482
GlaI GCGC 1 cut(s) 471
GluI GCNGC 5 cut(s) 28, 228, 273, 372, 482
HaeIII GGCC 2 cut(s) 307, 523
HapII CCGG 2 cut(s) 105, 520
HhaI GCGC 1 cut(s) 472
Hin1II CATG 2 cut(s) 425, 457
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HindIII AAGCTT 1 cut(s) 144
HinfI GANTC 1 cut(s) 71
HpaII CCGG 2 cut(s) 105, 520
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 2 cut(s) 21, 110
Hpy188III TCNNGA 1 cut(s) 444
Hpy8I GTNNAC 2 cut(s) 21, 110
Hpy99I CGWCG 1 cut(s) 102
HpyCH4V TGCA 6 cut(s) 21, 110, 167, 275, 374, 425
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 27, 101, 116, 233, 478
Hsp92II CATG 2 cut(s) 425, 457
HspAI GCGC 1 cut(s) 470
Kzo9I GATC 1 cut(s) 265
Lsp1109I GCAGC 4 cut(s) 14, 259, 358, 493
MaeIII GTNAC 1 cut(s) 394
MalI GATC 1 cut(s) 267
MbiI CCGCTC 1 cut(s) 230
MboI GATC 1 cut(s) 265
MboII GAAGA 4 cut(s) 197, 285, 288, 422
MfeI CAATTG 1 cut(s) 130
MhlI GDGCHC 2 cut(s) 23, 112
MluCI AATT 5 cut(s) 130, 190, 310, 315, 327
MnlI CCTC 3 cut(s) 301, 460, 527
MseI TTAA 5 cut(s) 48, 125, 176, 245, 314
MspI CCGG 2 cut(s) 105, 520
MspR9I CCNGG 1 cut(s) 354
MunI CAATTG 1 cut(s) 130
MvaI CCWGG 1 cut(s) 354
MvnI CGCG 1 cut(s) 470
MwoI GCNNNNNNNGC 6 cut(s) 18, 27, 101, 116, 233, 478
NdeI CATATG 1 cut(s) 486
NdeII GATC 1 cut(s) 265
NlaIII CATG 2 cut(s) 425, 457
NlaIV GGNNCC 3 cut(s) 306, 448, 477
NspI RCATGY 1 cut(s) 425
NspV TTCGAA 1 cut(s) 492
PaeI GCATGC 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 231
PasI CCCWGGG 1 cut(s) 353
PfeI GAWTC 1 cut(s) 71
PflMI CCANNNNNTGG 1 cut(s) 472
PkrI GCNGC 5 cut(s) 29, 229, 274, 373, 483
Psp6I CCWGG 1 cut(s) 352
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 3 cut(s) 306, 448, 477
PspPI GGNCC 1 cut(s) 305
PspXI VCTCGAGB 1 cut(s) 231
SaqAI TTAA 5 cut(s) 48, 125, 176, 245, 314
SatI GCNGC 5 cut(s) 28, 228, 273, 372, 482
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 1 cut(s) 305
ScrFI CCNGG 1 cut(s) 354
SduI GDGCHC 2 cut(s) 23, 112
SetI ASST 8 cut(s) 97, 118, 148, 166, 280, 325, 437, 452
Sfr274I CTCGAG 1 cut(s) 231
SfuI TTCGAA 1 cut(s) 492
SlaI CTCGAG 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
SphI GCATGC 1 cut(s) 425
Sse9I AATT 5 cut(s) 130, 190, 310, 315, 327
SsiI CCGC 3 cut(s) 197, 228, 479
StyD4I CCNGG 1 cut(s) 352
TaqI TCGA 3 cut(s) 100, 232, 492
TasI AATT 5 cut(s) 130, 190, 310, 315, 327
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 1 cut(s) 71
Tru1I TTAA 5 cut(s) 48, 125, 176, 245, 314
Tru9I TTAA 5 cut(s) 48, 125, 176, 245, 314
TseI GCWGC 4 cut(s) 27, 272, 371, 481
TspDTI ATGAA 1 cut(s) 63
Van91I CCANNNNNTGG 1 cut(s) 472
VneI GTGCAC 2 cut(s) 19, 108
XapI RAATTY 1 cut(s) 310
XceI RCATGY 1 cut(s) 425
XhoI CTCGAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.