MD02G1289500.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000257
Physical Location & Seq
Forward (+)
34577143 .. 34579326
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1289500.v1.1.491

Sequence Viewer

Length: 2184 bp
ATGGCAAAGCATGGAGCTTTCCTGTTTTCTTATGCTTTCTCCATTATTCTCTTGCTCACTGTGAGTTTACCGTCTGAAGCCGCTGATCAGGAAGAAAGAAAGACATACATTATCTACTTGGGATCACTTCCTAAGGATGAGCTGTTCTCGCCATTGTCTCACCACATTGGTATTCTCGAAAGAGTTACCGAGAGCAGCTCTGCTTCGAATCTCTTGGTCAGAAGCTACAAAAAAAGCTTCAACGGATTCGCTGCAAAGCTCACTGACCGAGAAGGAGAAACGCTTGCTAAGATGAAGGAAGTTGTCTCTGTGTTTCCGAGCAAAACTTACAAACTCCAAACAACAAGATCCTGGGAATTCTTGGGCTTCAATGAGAAGCACAAACGAAATCCTACTGTCGAGAGCAATGTTATTATTGGTGTCATCGATAGTGGGATATGGCCTGAATCTAAAAGTTTTAGCGATGAAGGTTTTGGTCCAGCTCCGAAGAAGTGGAAAGGAGTTTGCAAAGGAGGCAAAAATTTCACTTGCAACAACAAGATCATTGGAGCTCGGTTTTACACTGATTCCTCAGCCAGAGATGAGAATGGTCATGGAACTCATACTGCCTCAACCGCAGCAGGAAATCCGGTAAAGGGTGTGAGCTTTTATGGACTGGGGCAGGGAACTGCAAGAGGAGGCGTTCCCTCTGCGAGGATTGCTGCATATAAAGTTTGCAATTCTGAAGGTTGCTCTTCAGAAGGGCTCTTGGGTGCTTTTGATGACGCTATTCATGATGGAGTTGACATCATTACAATTTCAATTGGAGCCTACTCTGCAGAACCTTTGGATCAGGATCCTATAGCAATCGGTGCTCTTCATGCAATTGAGAAAGGGATACTAACCTTGCAATCTGCAGGAAATAGTGGTCCTGACGGAGGTTCTGTAGCAAGTGTAGCACCATGGATTCTTGCAGTTGCAGCAAGTAGCACAGATCGTCGCATTATTGACAAGGTTATTCTTGGAAACAGAAGGACGCTTGTCGGTAGTTCAGTGAATGCTTTCAAATTAAATGGAACAAGTTTTCCGTTGATAGAAGGAAAAAAGGCAAGTCATTGCTTCTTGGATTGTGAAATAGGTTGCCTGGACCCTAATTTAGCTAAGGGAAAGATTGTGGTATGTTCTGCTGCTAGGGGCAGTATTGAGGCTTATCGAGTCGGTGCAATAGGTGCGCTAACTAGCACTACCGAACCCGATGTTTCTTTCATTGTCCCACTACCTGCAGCAAGTTTTGACAGTAAAGATTTTGGCGCTGTCGAGTCGTATCTCAGCTCCACAGCCAACCCTCGAGCAAAGATACTAAAAAGTGAAGTCATAAAAGATCTTGCTGCACCTACTATCGCTTCCTTCTCTTCACGGGGGCCTAATTCTATTTTACCTGAAATTATCAAGCCGGACATAACTGCCCCGGGGGTTGATATTTTGGCTGCATATTCACCTGTTGCTCCTCTCATAGAGAGCTCTGAAGACCAGAGGCATGTAAAATACAGTATCTTATCTGGAACCTCCATGTCTTGCCCGCATGCTGCTGGTGTCGCTGCGTATATCAAGACATTCCACCCCAACTGGTCCCCATCTGCTATCAAATCATCTATTCTTACTACAGCTGGGCCTGTGAATGGCACCAACAATTCCACCTCACCGGGTGAATTTTCGTATGGATCTGGGCATATCAATCCTGTAAAAGCTGTAAATCCAGGGCTTGTGTACGAAACTTCTAAGAATGATTACATTAAGATGCTATGCTCGACCTTGGATGAGTCCAAAGTTCGACTTATTTCTGGAGACAAAGCCCCTTGCTCTGCAAGTACTGGCAATAAATCGGCCAAGGATCTAAACTACCCTTCACTGGCTAGTAATGTTTCACCAGGAACTTCATTTTCGGTTAAGTTTCAAAGAACAGTAAAAAATGTTGGCGTTGCAAACTCCACATACAAGGCAAAGATCATCTCAAAGCCGGGAGTTAACATCAAGGTGGTGCCTCAAGTTCTTTCCTTCAAGTCTTTGAATGAGAAAAAGACCTTCAATGTGACAGTTTCCGGAAGAGGTATGCCAAATCGTTCGCATGTATCTGCGTCGTTGGTGTGGACGGATGGCACGCACAGCGTTCGAAGTCCAATTCTTGTAACCAGCTTCTCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

728

Amino Acids

76.88

Weight (kDa)

9.05

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 113 7.1e-16 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 557 1e-45 Subtilase family
fn3_6 PF17766 624 - 722 1.4e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1267
AccB1I GGYRCC 2 cut(s) 1661, 2017
AccIII TCCGGA 1 cut(s) 2078
AciI CCGC 3 cut(s) 81, 615, 1559
AclWI GGATC 7 cut(s) 130, 342, 830, 837, 843, 1708, 1878
AcoI YGGCCR 1 cut(s) 1863
AcsI RAATTY 3 cut(s) 356, 520, 1688
AcuI CTGAAG 4 cut(s) 96, 720, 744, 1524
AdeI CACNNNGTG 1 cut(s) 1685
AfaI GTAC 2 cut(s) 1748, 1849
AfiI CCNNNNNNNGG 5 cut(s) 635, 693, 917, 1658, 1888
AgsI TTSAA 8 cut(s) 241, 370, 801, 1045, 1934, 2038, 2047, 2065
AjnI CCWGG 4 cut(s) 350, 1122, 1735, 1906
AloI GAACNNNNNNTCC 4 cut(s) 128, 160, 1048, 1080
Alw21I GWGCWC 3 cut(s) 553, 856, 1502
Alw26I GTCTC 3 cut(s) 162, 310, 1818
AlwI GGATC 7 cut(s) 130, 342, 830, 837, 843, 1708, 1878
Ama87I CYCGRG 2 cut(s) 1326, 1447
Aor13HI TCCGGA 1 cut(s) 2078
AoxI GGCC 4 cut(s) 440, 1400, 1649, 1863
ApoI RAATTY 3 cut(s) 356, 520, 1688
Asp700I GAANNNNTTC 1 cut(s) 1040
AspLEI GCGC 2 cut(s) 1213, 1292
AspS9I GGNCC 6 cut(s) 476, 908, 1126, 1400, 1608, 1649
AsuC2I CCSGG 4 cut(s) 1448, 1449, 1683, 1998
AsuHPI GGTGA 5 cut(s) 152, 1467, 1671, 1697, 1896
AsuII TTCGAA 2 cut(s) 206, 2149
AvaI CYCGRG 2 cut(s) 1326, 1447
AvaII GGWCC 4 cut(s) 476, 908, 1126, 1608
AxyI CCTNAGG 1 cut(s) 132
BamHI GGATCC 1 cut(s) 835
BanI GGYRCC 2 cut(s) 1661, 2017
BanII GRGCYC 3 cut(s) 553, 747, 1502
BbsI GAAGAC 1 cut(s) 1512
Bbv12I GWGCWC 3 cut(s) 553, 856, 1502
BbvCI CCTCAGC 1 cut(s) 571
BccI CCATC 3 cut(s) 770, 1621, 2126
BcgI CGANNNNNNTGC 2 cut(s) 2129, 2163
BciT130I CCWGG 4 cut(s) 352, 1124, 1737, 1908
BciVI GTATCC 1 cut(s) 870
BclI TGATCA 1 cut(s) 85
BcnI CCSGG 4 cut(s) 1448, 1449, 1683, 1998
BcoDI GTCTC 3 cut(s) 162, 310, 1818
BfaI CTAG 3 cut(s) 1170, 1218, 1893
BfmI CTRYAG 6 cut(s) 816, 840, 894, 924, 1260, 1641
BfoI RGCGCY 1 cut(s) 1293
BfuAI ACCTGC 1 cut(s) 1267
BfuI GTATCC 1 cut(s) 870
BglII AGATCT 1 cut(s) 1360
BmcAI AGTACT 1 cut(s) 1849
Bme1390I CCNGG 8 cut(s) 352, 1124, 1448, 1449, 1683, 1737, 1908, 1998
Bme18I GGWCC 4 cut(s) 476, 908, 1126, 1608
BmeT110I CYCGRG 2 cut(s) 1326, 1447
BmgT120I GGNCC 6 cut(s) 476, 908, 1126, 1400, 1608, 1649
BmiI GGNNCC 8 cut(s) 808, 837, 1128, 1401, 1543, 1610, 1663, 2019
BmrFI CCNGG 8 cut(s) 352, 1124, 1448, 1449, 1683, 1737, 1908, 1998
BmrI ACTGGG 1 cut(s) 665
BmsI GCATC 1 cut(s) 1767
BmuI ACTGGG 1 cut(s) 665
BoxI GACNNNNGTC 1 cut(s) 1019
BpiI GAAGAC 1 cut(s) 1512
BplI GAGNNNNNCTC 4 cut(s) 131, 163, 182, 214
BpmI CTGGAG 1 cut(s) 1842
Bpu10I CCTNAGC 2 cut(s) 571, 1140
Bpu14I TTCGAA 2 cut(s) 206, 2149
BpuEI CTTGAG 1 cut(s) 2007
BpuMI CCSGG 4 cut(s) 1448, 1449, 1683, 1998
Bsa29I ATCGAT 1 cut(s) 426
BsaBI GATNNNNATC 1 cut(s) 834
BsaJI CCNNGG 8 cut(s) 351, 941, 1446, 1447, 1448, 1736, 1791, 1866
BsaWI WCCGGW 2 cut(s) 628, 2078
BsaXI ACNNNNNCTCC 2 cut(s) 909, 939
Bsc4I CCNNNNNNNGG 5 cut(s) 635, 693, 917, 1658, 1888
Bse1I ACTGG 4 cut(s) 660, 1610, 1855, 1893
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 2 cut(s) 412, 1093
Bse8I GATNNNNATC 1 cut(s) 834
BseAI TCCGGA 1 cut(s) 2078
BseBI CCWGG 4 cut(s) 352, 1124, 1737, 1908
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 8 cut(s) 351, 941, 1446, 1447, 1448, 1736, 1791, 1866
BseGI GGATG 3 cut(s) 142, 1801, 2137
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 5 cut(s) 635, 693, 917, 1658, 1888
BseMI GCAATG 2 cut(s) 412, 1093
BseMII CTCAG 2 cut(s) 585, 1321
BseNI ACTGG 4 cut(s) 660, 1610, 1855, 1893
BseRI GAGGAG 2 cut(s) 690, 1476
BseYI CCCAGC 1 cut(s) 1646
BsgI GTGCAG 1 cut(s) 1353
BshFI GGCC 4 cut(s) 442, 1402, 1651, 1865
BshNI GGYRCC 2 cut(s) 1661, 2017
BshVI ATCGAT 1 cut(s) 426
BsiHKAI GWGCWC 3 cut(s) 553, 856, 1502
BsiHKCI CYCGRG 2 cut(s) 1326, 1447
BsiSI CCGG 6 cut(s) 629, 1433, 1448, 1682, 1997, 2079
BslFI GGGAC 2 cut(s) 1235, 1594
BslI CCNNNNNNNGG 5 cut(s) 635, 693, 917, 1658, 1888
BsmAI GTCTC 3 cut(s) 162, 310, 1818
BsmFI GGGAC 2 cut(s) 1235, 1594
BsmI GAATGC 1 cut(s) 1042
BsnI GGCC 4 cut(s) 442, 1402, 1651, 1865
BsoBI CYCGRG 2 cut(s) 1326, 1447
Bsp119I TTCGAA 2 cut(s) 206, 2149
Bsp1286I GDGCHC 4 cut(s) 553, 747, 856, 1502
Bsp13I TCCGGA 1 cut(s) 2078
Bsp19I CCATGG 1 cut(s) 941
BspACI CCGC 3 cut(s) 81, 615, 1559
BspANI GGCC 4 cut(s) 442, 1402, 1651, 1865
BspCNI CTCAG 2 cut(s) 584, 1320
BspDI ATCGAT 1 cut(s) 426
BspEI TCCGGA 1 cut(s) 2078
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 8 cut(s) 808, 837, 1128, 1401, 1543, 1610, 1663, 2019
BspMAI CTGCAG 3 cut(s) 820, 898, 1264
BspMI ACCTGC 1 cut(s) 1267
BspPI GGATC 7 cut(s) 130, 342, 830, 837, 843, 1708, 1878
BspQI GCTCTTC 2 cut(s) 739, 861
BspT104I TTCGAA 2 cut(s) 206, 2149
BspT107I GGYRCC 2 cut(s) 1661, 2017
BsrDI GCAATG 2 cut(s) 412, 1093
BsrI ACTGG 4 cut(s) 660, 1610, 1855, 1893
BssECI CCNNGG 8 cut(s) 351, 941, 1446, 1447, 1448, 1736, 1791, 1866
BssT1I CCWWGG 3 cut(s) 941, 1791, 1866
Bst2UI CCWGG 4 cut(s) 352, 1124, 1737, 1908
Bst4CI ACNGT 7 cut(s) 61, 72, 397, 1277, 1529, 1942, 2074
Bst6I CTCTTC 4 cut(s) 739, 861, 1396, 2077
BstAPI GCANNNNNTGC 2 cut(s) 851, 1208
BstBI TTCGAA 2 cut(s) 206, 2149
BstC8I GCNNGC 4 cut(s) 285, 1559, 1563, 2138
BstDEI CTNAG 6 cut(s) 132, 288, 571, 1140, 1307, 1758
BstDSI CCRYGG 1 cut(s) 941
BstENI CCTNNNNNAGG 2 cut(s) 691, 915
BstF5I GGATG 3 cut(s) 142, 1801, 2137
BstH2I RGCGCY 1 cut(s) 1293
BstHHI GCGC 2 cut(s) 1213, 1292
BstMAI GTCTC 3 cut(s) 162, 310, 1818
BstNI CCWGG 4 cut(s) 352, 1124, 1737, 1908
BstNSI RCATGY 3 cut(s) 1520, 1565, 2108
BstPAI GACNNNNGTC 1 cut(s) 1019
BstSCI CCNGG 8 cut(s) 350, 1122, 1446, 1447, 1681, 1735, 1906, 1996
BstSFI CTRYAG 6 cut(s) 816, 840, 894, 924, 1260, 1641
BstV2I GAAGAC 1 cut(s) 1512
BstX2I RGATCY 5 cut(s) 347, 835, 1360, 1700, 1870
BstYI RGATCY 5 cut(s) 347, 835, 1360, 1700, 1870
Bsu15I ATCGAT 1 cut(s) 426
Bsu36I CCTNAGG 1 cut(s) 132
BsuI GTATCC 1 cut(s) 870
BsuRI GGCC 4 cut(s) 442, 1402, 1651, 1865
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 941
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 3 cut(s) 142, 1801, 2137
BtsIMutI CAGTG 5 cut(s) 57, 261, 561, 1038, 1886
BveI ACCTGC 1 cut(s) 1267
Cac8I GCNNGC 4 cut(s) 285, 1559, 1563, 2138
CciI TCATGA 1 cut(s) 772
CfoI GCGC 2 cut(s) 1213, 1292
Cfr13I GGNCC 6 cut(s) 476, 908, 1126, 1400, 1608, 1649
Cfr9I CCCGGG 1 cut(s) 1447
ClaI ATCGAT 1 cut(s) 426
CseI GACGC 3 cut(s) 773, 1024, 2103
Csp6I GTAC 2 cut(s) 1747, 1848
CviAII CATG 9 cut(s) 11, 593, 773, 860, 942, 1517, 1549, 1562, 2105
CviQI GTAC 2 cut(s) 1747, 1848
DdeI CTNAG 6 cut(s) 132, 288, 571, 1140, 1307, 1758
DraIII CACNNNGTG 1 cut(s) 1685
EaeI YGGCCR 1 cut(s) 1863
Eam1104I CTCTTC 4 cut(s) 739, 861, 1396, 2077
EarI CTCTTC 4 cut(s) 739, 861, 1396, 2077
Ecl136II GAGCTC 2 cut(s) 551, 1500
Eco130I CCWWGG 3 cut(s) 941, 1791, 1866
Eco24I GRGCYC 3 cut(s) 553, 747, 1502
Eco47I GGWCC 4 cut(s) 476, 908, 1126, 1608
Eco53kI GAGCTC 2 cut(s) 551, 1500
Eco57I CTGAAG 4 cut(s) 96, 720, 744, 1524
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 2 cut(s) 1326, 1447
EcoICRI GAGCTC 2 cut(s) 551, 1500
EcoNI CCTNNNNNAGG 2 cut(s) 691, 915
EcoO109I RGGNCCY 1 cut(s) 1400
EcoRI GAATTC 1 cut(s) 356
EcoRII CCWGG 4 cut(s) 350, 1122, 1735, 1906
EcoT14I CCWWGG 3 cut(s) 941, 1791, 1866
EcoT38I GRGCYC 3 cut(s) 553, 747, 1502
ErhI CCWWGG 3 cut(s) 941, 1791, 1866
FaeI CATG 9 cut(s) 14, 596, 776, 863, 945, 1520, 1552, 1565, 2108
FalI AAGNNNNNCTT 2 cut(s) 1797, 1829
FaqI GGGAC 2 cut(s) 1235, 1594
FatI CATG 9 cut(s) 10, 592, 772, 859, 941, 1516, 1548, 1561, 2104
FauI CCCGC 1 cut(s) 1566
FbaI TGATCA 1 cut(s) 85
FokI GGATG 3 cut(s) 149, 1808, 2144
FriOI GRGCYC 3 cut(s) 553, 747, 1502
FspBI CTAG 3 cut(s) 1170, 1218, 1893
GlaI GCGC 2 cut(s) 1212, 1291
GsaI CCCAGC 1 cut(s) 1650
GsuI CTGGAG 1 cut(s) 1842
HaeII RGCGCY 1 cut(s) 1293
HaeIII GGCC 4 cut(s) 442, 1402, 1651, 1865
HapII CCGG 6 cut(s) 629, 1433, 1448, 1682, 1997, 2079
HgaI GACGC 3 cut(s) 773, 1024, 2103
HhaI GCGC 2 cut(s) 1213, 1292
Hin1II CATG 9 cut(s) 14, 596, 776, 863, 945, 1520, 1552, 1565, 2108
Hin6I GCGC 2 cut(s) 1211, 1290
HinP1I GCGC 2 cut(s) 1211, 1290
HincII GTYRAC 2 cut(s) 784, 2005
HindII GTYRAC 2 cut(s) 784, 2005
HindIII AAGCTT 1 cut(s) 235
HinfI GANTC 8 cut(s) 208, 246, 446, 566, 946, 1194, 1298, 1799
HpaI GTTAAC 1 cut(s) 2005
HpaII CCGG 6 cut(s) 629, 1433, 1448, 1682, 1997, 2079
HphI GGTGA 5 cut(s) 152, 1467, 1671, 1697, 1896
Hpy166II GTNNAC 5 cut(s) 68, 784, 1747, 2005, 2127
Hpy188I TCNGA 7 cut(s) 76, 221, 318, 486, 724, 739, 1504
Hpy8I GTNNAC 5 cut(s) 68, 784, 1747, 2005, 2127
Hpy99I CGWCG 2 cut(s) 981, 2119
HpyCH4III ACNGT 7 cut(s) 61, 72, 397, 1277, 1529, 1942, 2074
HpyF3I CTNAG 6 cut(s) 132, 288, 571, 1140, 1307, 1758
Hsp92II CATG 9 cut(s) 14, 596, 776, 863, 945, 1520, 1552, 1565, 2108
HspAI GCGC 2 cut(s) 1211, 1290
Kpn2I TCCGGA 1 cut(s) 2078
Ksp22I TGATCA 1 cut(s) 85
KspAI GTTAAC 1 cut(s) 2005
LguI GCTCTTC 2 cut(s) 739, 861
LmnI GCTCC 6 cut(s) 14, 487, 548, 806, 1316, 1489
LweI GCATC 1 cut(s) 1767
MaeI CTAG 3 cut(s) 1170, 1218, 1893
MaeIII GTNAC 3 cut(s) 184, 2068, 2164
MboII GAAGA 7 cut(s) 104, 499, 726, 848, 1383, 1517, 2094
MfeI CAATTG 2 cut(s) 801, 864
MflI RGATCY 5 cut(s) 347, 835, 1360, 1700, 1870
MhlI GDGCHC 4 cut(s) 553, 747, 856, 1502
MlyI GAGTC 3 cut(s) 1203, 1307, 1808
MroI TCCGGA 1 cut(s) 2078
MroXI GAANNNNTTC 1 cut(s) 1040
MseI TTAA 4 cut(s) 1049, 1773, 1926, 2004
MslI CAYNNNNRTG 2 cut(s) 1775, 2012
MspA1I CMGCKG 2 cut(s) 83, 1646
MspI CCGG 6 cut(s) 629, 1433, 1448, 1682, 1997, 2079
MspR9I CCNGG 8 cut(s) 352, 1124, 1448, 1449, 1683, 1737, 1908, 1998
MunI CAATTG 2 cut(s) 801, 864
Mva1269I GAATGC 1 cut(s) 1042
MvaI CCWGG 4 cut(s) 352, 1124, 1737, 1908
NciI CCSGG 4 cut(s) 1448, 1449, 1683, 1998
NcoI CCATGG 1 cut(s) 941
NlaIII CATG 9 cut(s) 14, 596, 776, 863, 945, 1520, 1552, 1565, 2108
NlaIV GGNNCC 8 cut(s) 808, 837, 1128, 1401, 1543, 1610, 1663, 2019
NmuCI GTSAC 1 cut(s) 2068
NspI RCATGY 3 cut(s) 1520, 1565, 2108
NspV TTCGAA 2 cut(s) 206, 2149
PaeI GCATGC 1 cut(s) 1565
PaeR7I CTCGAG 1 cut(s) 1326
PagI TCATGA 1 cut(s) 772
PciSI GCTCTTC 2 cut(s) 739, 861
PctI GAATGC 1 cut(s) 1042
PdmI GAANNNNTTC 1 cut(s) 1040
PfeI GAWTC 5 cut(s) 208, 246, 446, 566, 946
PleI GAGTC 3 cut(s) 1202, 1306, 1807
PpsI GAGTC 3 cut(s) 1202, 1306, 1807
PshAI GACNNNNGTC 1 cut(s) 1019
Psp124BI GAGCTC 2 cut(s) 553, 1502
Psp6I CCWGG 4 cut(s) 350, 1122, 1735, 1906
PspFI CCCAGC 1 cut(s) 1646
PspGI CCWGG 4 cut(s) 350, 1122, 1735, 1906
PspN4I GGNNCC 8 cut(s) 808, 837, 1128, 1401, 1543, 1610, 1663, 2019
PspPI GGNCC 6 cut(s) 476, 908, 1126, 1400, 1608, 1649
PspXI VCTCGAGB 1 cut(s) 1326
PstI CTGCAG 3 cut(s) 820, 898, 1264
PsuI RGATCY 5 cut(s) 347, 835, 1360, 1700, 1870
PvuII CAGCTG 1 cut(s) 1646
RsaI GTAC 2 cut(s) 1748, 1849
RsaNI GTAC 2 cut(s) 1747, 1848
RseI CAYNNNNRTG 2 cut(s) 1775, 2012
SacI GAGCTC 2 cut(s) 553, 1502
SapI GCTCTTC 2 cut(s) 739, 861
SaqAI TTAA 4 cut(s) 1049, 1773, 1926, 2004
Sau96I GGNCC 6 cut(s) 476, 908, 1126, 1400, 1608, 1649
ScaI AGTACT 1 cut(s) 1849
SchI GAGTC 3 cut(s) 1203, 1307, 1808
ScrFI CCNGG 8 cut(s) 352, 1124, 1448, 1449, 1683, 1737, 1908, 1998
SduI GDGCHC 4 cut(s) 553, 747, 856, 1502
SfaNI GCATC 1 cut(s) 1767
SfcI CTRYAG 6 cut(s) 816, 840, 894, 924, 1260, 1641
Sfr274I CTCGAG 1 cut(s) 1326
SfuI TTCGAA 2 cut(s) 206, 2149
SinI GGWCC 4 cut(s) 476, 908, 1126, 1608
SlaI CTCGAG 1 cut(s) 1326
SmaI CCCGGG 1 cut(s) 1449
SmiMI CAYNNNNRTG 2 cut(s) 1775, 2012
SmlI CTYRAG 2 cut(s) 1326, 2022
SmoI CTYRAG 2 cut(s) 1326, 2022
SphI GCATGC 1 cut(s) 1565
SsiI CCGC 3 cut(s) 81, 615, 1559
SspMI CTAG 3 cut(s) 1170, 1218, 1893
SstI GAGCTC 2 cut(s) 553, 1502
StyD4I CCNGG 8 cut(s) 350, 1122, 1446, 1447, 1681, 1735, 1906, 1996
StyI CCWWGG 3 cut(s) 941, 1791, 1866
TaaI ACNGT 7 cut(s) 61, 72, 397, 1277, 1529, 1942, 2074
TaqII GACCGA 1 cut(s) 282
TatI WGTACW 1 cut(s) 1847
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 5 cut(s) 208, 246, 446, 566, 946
Tru1I TTAA 4 cut(s) 1049, 1773, 1926, 2004
Tru9I TTAA 4 cut(s) 1049, 1773, 1926, 2004
TscAI CASTG 5 cut(s) 64, 268, 568, 1038, 1893
TseFI GTSAC 1 cut(s) 2068
Tsp45I GTSAC 1 cut(s) 2068
TspDTI ATGAA 6 cut(s) 308, 480, 761, 848, 1234, 1905
TspGWI ACGGA 4 cut(s) 258, 930, 1056, 2144
TspMI CCCGGG 1 cut(s) 1447
TspRI CASTG 5 cut(s) 64, 268, 568, 1038, 1893
VpaK11BI GGWCC 4 cut(s) 476, 908, 1126, 1608
XagI CCTNNNNNAGG 2 cut(s) 691, 915
XapI RAATTY 3 cut(s) 356, 520, 1688
XceI RCATGY 3 cut(s) 1520, 1565, 2108
XhoI CTCGAG 1 cut(s) 1326
XmaI CCCGGG 1 cut(s) 1447
XmnI GAANNNNTTC 1 cut(s) 1040
XspI CTAG 3 cut(s) 1170, 1218, 1893
ZrmI AGTACT 1 cut(s) 1849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.