Prupe.2G033100_v2.0.a1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000257
Physical Location & Seq
Reverse (-)
3559080 .. 3561221
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G033100.1

Sequence Viewer

Length: 2142 bp
ATGAGCTTATTGTGCAAAGCCATTGATGATGAAGACAGAAAGCCATATATTGTCTACTTGGGATCACTTCCTAATGATGAGGCCTTCTCACCATTGTCTCACCAAATCGGTATACTCGAAAGAGTTGTGGAGAGCACTTCTGCTTCTGATTTCTTGATAAGAAGCTACAAAAGGAGCTTCAATGGGTTTGCTGCCAAGCTCACTGACCAAGAAATAGAAAGGCTTGCTAACATGAAGGAAGTAGTCTCTGTCTTTCCAAGCACAAACTTCCAACTTCACACAACAAGATCTTGGGATTTTACTGGTCTCAATGACGAAACCAAACGAAATTCTACTACTGAGAGTGATGTTGTCATTGGTGTAATCGACACTGGAATTTGGCCCGAATCGCAGAGCTTCAATGACGAAGGTTTTAGTCCGGCTCCCAAGAAGTGGAAAGGTGTTTGTGAAGGTGGCAAAAATTTCACTTGCAACAACAAGATCATTGGAGCTCGGCGTTACAACTCATCATCAGCAAGGGATGAAATTGGACATGGATCCCACACTGCTTCAACGGCAGCAGGGAATGCCGTTAAGGGCGCGAGCTTTTATGGTCTAGCACAAGGTACTGCAAGAGGAGGAGTTCCCTCTGCGAGAATTGCTGCCTATAAAGTCTGCGAATTCGATAAGTGCCCCGGAGAGGCTATCATGGCTGCTTTTGACGACGCTATTGCTGATGGAGTTGACATCATTACAATTTCACTTGGAGGCACTTTCGTCACTTCTTTCGATAAGGATCCTATAGCCATTGGCAGTTTTCATGCAATGAAGAAAGGGATACTAACCGCACATTCTGCAGGCAACAGTGGCCCTGAAGAGGGTACCGTAGTAAGTGTAGAACCATGGGTACTTACAGTTGCAGCAAGTGGCACAGATCGCCGGATCATTGACAAGGTTGTTCTTGGAAATGGAAGGACACTCATCGGGAATTCGGTGAACTCTTTCACATCCAATGGAACAAGTTATCCTTTGGTATATGGAAAAGATGCTACAAGTCATTGCTCCAATTTCGATGCTCAGAGTTGTTTAGCAGGCTGCATAGACAGTGATTTAGTCAAGGGAAAGATTTTGGTATGTGATGCGTCTGATGGAGATATAGTGGCTCGTCAAGCTGGTGCACGAGGTTCCATTGTAATCAGTCCCAGTGAGGATGTTGCTTTTATTGTCCCACTACCTGCAACAGGTTTAAGCATCAAAGATTATGAGGGGCTCAAGTCCTACCTGAACTCCACAAAACATGCTAAAGCCAACATATTAAAAAGTGAAGCCATAAAAGATACTGCTGCACCAATTGTTGTTTCTTTCTCTTCCCGTGGACCGAATTCGATTTTACCAGAAATTATTAAACCAGACATAAGCGCCCCAGGAGTAGATATTTTGGCTGCATTTTCGCCTGTTGCTGCTATCACAGATAGCCCTGACGACAGGAGGCATGTCAAATACAGTCTACTATCTGGAACCTCCATGGCTTGTCCCCATGCGGCTGCTGCAGCTGCATATATTAGAACATTCCACCCTGAATGGTCTCCAGCAGCCATCAAATCATCTCTTATGACTACAGCTTGGCCCATGAACCACACCGACGACGTTTCTCCTGCTGAATTTGCTCATGGATCTGGACACATCAATCCTGTAACAGCTCTAGACCCTGGCCTCGTGTACGAAACTTCTGAAGGTGATCACATTAAGTTGCTCTGCTCGTTCCTGGATGATGCAAGAGTTAAACTTATATCGGGAGAGAACAGCTCTTGTCCTAAAGGCTCTGAGAAAGGATCACCAAAGGATTTCAATTACCCTTCGCTGGCTGCTGTTGTTAAACCAGTGACATCTTTTACTATTAACTTTAATAGAACAGTTAAAAACGTTGGCCTTGCAAACTCTACCTACAAGGCCAAAATCCTCCCGGATTCTAAAGTTGACATCAAAGTAGTACCTCAAGTTCTGTCATTCAAGTCCTTGAATGAGGAGAAGACTTTCACCGTGACAGTCGTTGGAAAAGGCTTGCCGGTTGGATCACATGTGTCTGCATCGCTGGTTTGGTATGATGGAACTCATAGAGTTAGAAGTCCAATTCTTGTACACAGCAAAGCAGCTTCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

714

Amino Acids

75.85

Weight (kDa)

6.33

Isoelectric Point (pI)

35.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1222
Acc65I GGTACC 1 cut(s) 860
AccB1I GGYRCC 1 cut(s) 860
AccB7I CCANNNNNTGG 1 cut(s) 432
AccI GTMKAC 3 cut(s) 54, 112, 1486
AccII CGCG 1 cut(s) 581
AciI CCGC 2 cut(s) 825, 1520
AclI AACGTT 1 cut(s) 1902
AclWI GGATC 9 cut(s) 70, 531, 544, 770, 783, 929, 1660, 1819, 2059
AcsI RAATTY 7 cut(s) 328, 375, 460, 659, 967, 1360, 1640
AcuI CTGAAG 2 cut(s) 873, 1731
AfaI GTAC 6 cut(s) 607, 862, 888, 1700, 1971, 2118
AfiI CCNNNNNNNGG 6 cut(s) 432, 679, 856, 857, 1220, 1840
AflIII ACRYGT 1 cut(s) 2056
AgsI TTSAA 6 cut(s) 181, 400, 552, 1828, 1990, 1999
AjnI CCWGG 3 cut(s) 1402, 1687, 1743
AjuI GAANNNNNNNTTGG 2 cut(s) 1321, 1353
AloI GAACNNNNNNTCC 2 cut(s) 988, 1020
Alw21I GWGCWC 3 cut(s) 137, 493, 1159
Alw26I GTCTC 4 cut(s) 102, 250, 311, 1569
Alw44I GTGCAC 1 cut(s) 1155
AlwI GGATC 9 cut(s) 70, 531, 544, 770, 783, 929, 1660, 1819, 2059
AoxI GGCC 7 cut(s) 81, 380, 847, 1604, 1690, 1906, 1929
ApaLI GTGCAC 1 cut(s) 1155
ApoI RAATTY 7 cut(s) 328, 375, 460, 659, 967, 1360, 1640
Asp700I GAANNNNTTC 2 cut(s) 980, 2012
Asp718I GGTACC 1 cut(s) 860
AspLEI GCGC 2 cut(s) 581, 1400
AspS9I GGNCC 4 cut(s) 381, 848, 1355, 1605
AsuC2I CCSGG 2 cut(s) 675, 1943
AsuHPI GGTGA 6 cut(s) 81, 92, 985, 1727, 1806, 2008
AvaII GGWCC 1 cut(s) 1355
BaeGI GKGCMC 2 cut(s) 674, 1159
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
BamHI GGATCC 2 cut(s) 536, 775
BanI GGYRCC 1 cut(s) 860
BanII GRGCYC 2 cut(s) 493, 1251
BauI CACGAG 2 cut(s) 1158, 1694
BbsI GAAGAC 2 cut(s) 39, 2015
Bbv12I GWGCWC 3 cut(s) 137, 493, 1159
BccI CCATC 4 cut(s) 710, 1121, 1583, 2078
BceAI ACGGC 2 cut(s) 554, 570
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BciT130I CCWGG 3 cut(s) 1404, 1689, 1745
BciVI GTATCC 1 cut(s) 810
BclI TGATCA 1 cut(s) 1717
BcnI CCSGG 2 cut(s) 675, 1943
BcoDI GTCTC 4 cut(s) 102, 250, 311, 1569
BfaI CTAG 2 cut(s) 596, 1682
BfmI CTRYAG 4 cut(s) 780, 834, 1527, 1596
BfoI RGCGCY 1 cut(s) 1401
BfuAI ACCTGC 1 cut(s) 1222
BfuI GTATCC 1 cut(s) 810
BglII AGATCT 1 cut(s) 287
Bme1390I CCNGG 5 cut(s) 675, 1404, 1689, 1745, 1943
Bme18I GGWCC 1 cut(s) 1355
BmgT120I GGNCC 4 cut(s) 381, 848, 1355, 1605
BmiI GGNNCC 6 cut(s) 423, 538, 777, 862, 1165, 1498
BmrFI CCNGG 5 cut(s) 675, 1404, 1689, 1745, 1943
BmrI ACTGGG 1 cut(s) 1176
BmsI GCATC 6 cut(s) 1015, 1042, 1108, 1239, 1741, 2075
BmuI ACTGGG 1 cut(s) 1176
BpiI GAAGAC 2 cut(s) 39, 2015
BplI GAGNNNNNCTC 4 cut(s) 71, 103, 1769, 1801
BpmI CTGGAG 1 cut(s) 1551
BpuEI CTTGAG 2 cut(s) 1235, 1959
BpuMI CCSGG 2 cut(s) 675, 1943
BsaBI GATNNNNATC 1 cut(s) 774
BsaI GGTCTC 2 cut(s) 311, 1569
BsaJI CCNNGG 6 cut(s) 673, 881, 1351, 1402, 1503, 1687
BsaXI ACNNNNNCTCC 4 cut(s) 1122, 1152, 1250, 1280
Bsc4I CCNNNNNNNGG 6 cut(s) 432, 679, 856, 857, 1220, 1840
Bse118I RCCGGY 1 cut(s) 2044
Bse1I ACTGG 4 cut(s) 307, 376, 1182, 1859
Bse3DI GCAATG 2 cut(s) 810, 1036
Bse8I GATNNNNATC 1 cut(s) 774
BseBI CCWGG 3 cut(s) 1404, 1689, 1745
BseDI CCNNGG 6 cut(s) 673, 881, 1351, 1402, 1503, 1687
BseGI GGATG 4 cut(s) 526, 986, 1195, 1753
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 6 cut(s) 432, 679, 856, 857, 1220, 1840
BseMI GCAATG 2 cut(s) 810, 1036
BseMII CTCAG 3 cut(s) 330, 1070, 1794
BseNI ACTGG 4 cut(s) 307, 376, 1182, 1859
BseRI GAGGAG 3 cut(s) 630, 633, 2018
BseSI GKGCMC 2 cut(s) 674, 1159
BsgI GTGCAG 1 cut(s) 1308
Bsh1236I CGCG 1 cut(s) 581
BshFI GGCC 7 cut(s) 83, 382, 849, 1606, 1692, 1908, 1931
BshNI GGYRCC 1 cut(s) 860
BsiHKAI GWGCWC 3 cut(s) 137, 493, 1159
BsiSI CCGG 5 cut(s) 419, 675, 919, 1943, 2045
BslFI GGGAC 3 cut(s) 1164, 1190, 1497
BslI CCNNNNNNNGG 6 cut(s) 432, 679, 856, 857, 1220, 1840
BsmAI GTCTC 4 cut(s) 102, 250, 311, 1569
BsmFI GGGAC 3 cut(s) 1164, 1190, 1497
BsmI GAATGC 1 cut(s) 571
BsnI GGCC 7 cut(s) 83, 382, 849, 1606, 1692, 1908, 1931
Bso31I GGTCTC 2 cut(s) 311, 1569
Bsp1286I GDGCHC 5 cut(s) 137, 493, 674, 1159, 1251
Bsp1407I TGTACA 1 cut(s) 2116
Bsp19I CCATGG 2 cut(s) 881, 1503
BspACI CCGC 2 cut(s) 825, 1520
BspANI GGCC 7 cut(s) 83, 382, 849, 1606, 1692, 1908, 1931
BspCNI CTCAG 3 cut(s) 331, 1069, 1795
BspFNI CGCG 1 cut(s) 581
BspLI GGNNCC 6 cut(s) 423, 538, 777, 862, 1165, 1498
BspMAI CTGCAG 2 cut(s) 838, 1531
BspMI ACCTGC 1 cut(s) 1222
BspPI GGATC 9 cut(s) 70, 531, 544, 770, 783, 929, 1660, 1819, 2059
BspT107I GGYRCC 1 cut(s) 860
BspTNI GGTCTC 2 cut(s) 311, 1569
BsrDI GCAATG 2 cut(s) 810, 1036
BsrFI RCCGGY 1 cut(s) 2044
BsrGI TGTACA 1 cut(s) 2116
BsrI ACTGG 4 cut(s) 307, 376, 1182, 1859
BssAI RCCGGY 1 cut(s) 2044
BssECI CCNNGG 6 cut(s) 673, 881, 1351, 1402, 1503, 1687
BssNAI GTATAC 1 cut(s) 113
BssSI CACGAG 2 cut(s) 1158, 1694
BssT1I CCWWGG 2 cut(s) 881, 1503
Bst1107I GTATAC 1 cut(s) 113
Bst2BI CACGAG 2 cut(s) 1158, 1694
Bst2UI CCWGG 3 cut(s) 1404, 1689, 1745
Bst4CI ACNGT 8 cut(s) 845, 865, 895, 1085, 1484, 1894, 2020, 2026
Bst6I CTCTTC 2 cut(s) 849, 1351
BstAPI GCANNNNNTGC 2 cut(s) 566, 833
BstAUI TGTACA 1 cut(s) 2116
BstC8I GCNNGC 6 cut(s) 225, 583, 838, 1072, 1842, 2042
BstDEI CTNAG 3 cut(s) 339, 1056, 1803
BstDSI CCRYGG 3 cut(s) 881, 1351, 1503
BstENI CCTNNNNNAGG 1 cut(s) 1218
BstF5I GGATG 4 cut(s) 526, 986, 1195, 1753
BstFNI CGCG 1 cut(s) 581
BstH2I RGCGCY 1 cut(s) 1401
BstHHI GCGC 2 cut(s) 581, 1400
BstMAI GTCTC 4 cut(s) 102, 250, 311, 1569
BstNI CCWGG 3 cut(s) 1404, 1689, 1745
BstNSI RCATGY 3 cut(s) 1280, 1475, 2060
BstSCI CCNGG 5 cut(s) 673, 1402, 1687, 1743, 1941
BstSFI CTRYAG 4 cut(s) 780, 834, 1527, 1596
BstSLI GKGCMC 2 cut(s) 674, 1159
BstUI CGCG 1 cut(s) 581
BstV2I GAAGAC 2 cut(s) 39, 2015
BstX2I RGATCY 4 cut(s) 287, 536, 775, 1652
BstYI RGATCY 4 cut(s) 287, 536, 775, 1652
BstZ17I GTATAC 1 cut(s) 113
BsuI GTATCC 1 cut(s) 810
BsuRI GGCC 7 cut(s) 83, 382, 849, 1606, 1692, 1908, 1931
BtgI CCRYGG 3 cut(s) 881, 1351, 1503
BtgZI GCGATG 1 cut(s) 2052
BtsCI GGATG 4 cut(s) 526, 986, 1195, 1753
BtsI GCAGTG 1 cut(s) 543
BtsIMutI CAGTG 7 cut(s) 201, 369, 543, 850, 1090, 1189, 1866
BveI ACCTGC 1 cut(s) 1222
Cac8I GCNNGC 6 cut(s) 225, 583, 838, 1072, 1842, 2042
CfoI GCGC 2 cut(s) 581, 1400
Cfr10I RCCGGY 1 cut(s) 2044
Cfr13I GGNCC 4 cut(s) 381, 848, 1355, 1605
CseI GACGC 2 cut(s) 713, 1110
Csp6I GTAC 6 cut(s) 606, 861, 887, 1699, 1970, 2117
CviQI GTAC 6 cut(s) 606, 861, 887, 1699, 1970, 2117
DdeI CTNAG 3 cut(s) 339, 1056, 1803
Eam1104I CTCTTC 2 cut(s) 849, 1351
EarI CTCTTC 2 cut(s) 849, 1351
Ecl136II GAGCTC 1 cut(s) 491
Eco130I CCWWGG 2 cut(s) 881, 1503
Eco147I AGGCCT 1 cut(s) 83
Eco24I GRGCYC 2 cut(s) 493, 1251
Eco31I GGTCTC 2 cut(s) 311, 1569
Eco47I GGWCC 1 cut(s) 1355
Eco53kI GAGCTC 1 cut(s) 491
Eco57I CTGAAG 2 cut(s) 873, 1731
EcoICRI GAGCTC 1 cut(s) 491
EcoNI CCTNNNNNAGG 1 cut(s) 1218
EcoRI GAATTC 3 cut(s) 659, 967, 1360
EcoRII CCWGG 3 cut(s) 1402, 1687, 1743
EcoT14I CCWWGG 2 cut(s) 881, 1503
EcoT38I GRGCYC 2 cut(s) 493, 1251
ErhI CCWWGG 2 cut(s) 881, 1503
FalI AAGNNNNNCTT 2 cut(s) 991, 1023
FaqI GGGAC 3 cut(s) 1164, 1190, 1497
FbaI TGATCA 1 cut(s) 1717
FblI GTMKAC 3 cut(s) 54, 112, 1486
FokI GGATG 4 cut(s) 533, 973, 1202, 1760
FriOI GRGCYC 2 cut(s) 493, 1251
FspBI CTAG 2 cut(s) 596, 1682
GlaI GCGC 2 cut(s) 580, 1399
GsuI CTGGAG 1 cut(s) 1551
HaeII RGCGCY 1 cut(s) 1401
HaeIII GGCC 7 cut(s) 83, 382, 849, 1606, 1692, 1908, 1931
HapII CCGG 5 cut(s) 419, 675, 919, 1943, 2045
HgaI GACGC 2 cut(s) 713, 1110
HhaI GCGC 2 cut(s) 581, 1400
Hin6I GCGC 2 cut(s) 579, 1398
HinP1I GCGC 2 cut(s) 579, 1398
HincII GTYRAC 2 cut(s) 724, 1957
HindII GTYRAC 2 cut(s) 724, 1957
HinfI GANTC 2 cut(s) 386, 1946
HpaII CCGG 5 cut(s) 419, 675, 919, 1943, 2045
HphI GGTGA 6 cut(s) 81, 92, 985, 1727, 1806, 2008
Hpy188I TCNGA 5 cut(s) 148, 1059, 1126, 1711, 1804
Hpy188III TCNNGA 6 cut(s) 154, 964, 1494, 1656, 1682, 1773
Hpy99I CGWCG 3 cut(s) 707, 1625, 1628
HpyAV CCTTC 7 cut(s) 94, 229, 401, 443, 945, 1706, 1845
HpyCH4III ACNGT 8 cut(s) 845, 865, 895, 1085, 1484, 1894, 2020, 2026
HpyCH4IV ACGT 2 cut(s) 1626, 1902
HpyF3I CTNAG 3 cut(s) 339, 1056, 1803
HpySE526I ACGT 2 cut(s) 1626, 1902
HspAI GCGC 2 cut(s) 579, 1398
KpnI GGTACC 1 cut(s) 864
Ksp22I TGATCA 1 cut(s) 1717
LmnI GCTCC 4 cut(s) 174, 427, 488, 1046
LweI GCATC 6 cut(s) 1015, 1042, 1108, 1239, 1741, 2075
MaeI CTAG 2 cut(s) 596, 1682
MaeII ACGT 2 cut(s) 1626, 1902
MaeIII GTNAC 5 cut(s) 497, 757, 1672, 1861, 2020
MboII GAAGA 5 cut(s) 44, 820, 866, 1338, 2020
MfeI CAATTG 1 cut(s) 1329
MflI RGATCY 4 cut(s) 287, 536, 775, 1652
MhlI GDGCHC 5 cut(s) 137, 493, 674, 1159, 1251
MmeI TCCRAC 3 cut(s) 295, 2011, 2029
MroXI GAANNNNTTC 2 cut(s) 980, 2012
MspA1I CMGCKG 1 cut(s) 1532
MspI CCGG 5 cut(s) 419, 675, 919, 1943, 2045
MspR9I CCNGG 5 cut(s) 675, 1404, 1689, 1745, 1943
MunI CAATTG 1 cut(s) 1329
Mva1269I GAATGC 1 cut(s) 571
MvaI CCWGG 3 cut(s) 1404, 1689, 1745
MvnI CGCG 1 cut(s) 581
NciI CCSGG 2 cut(s) 675, 1943
NcoI CCATGG 2 cut(s) 881, 1503
NlaIV GGNNCC 6 cut(s) 423, 538, 777, 862, 1165, 1498
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 3 cut(s) 757, 1861, 2020
NspI RCATGY 3 cut(s) 1280, 1475, 2060
PceI AGGCCT 1 cut(s) 83
PciI ACATGT 1 cut(s) 2056
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 1 cut(s) 571
PdmI GAANNNNTTC 2 cut(s) 980, 2012
PfeI GAWTC 2 cut(s) 386, 1946
PflMI CCANNNNNTGG 1 cut(s) 432
PfoI TCCNGGA 2 cut(s) 1743, 1941
PscI ACATGT 1 cut(s) 2056
Psp124BI GAGCTC 1 cut(s) 493
Psp1406I AACGTT 1 cut(s) 1902
Psp6I CCWGG 3 cut(s) 1402, 1687, 1743
PspGI CCWGG 3 cut(s) 1402, 1687, 1743
PspN4I GGNNCC 6 cut(s) 423, 538, 777, 862, 1165, 1498
PspPI GGNCC 4 cut(s) 381, 848, 1355, 1605
PsrI GAACNNNNNNTAC 4 cut(s) 870, 902, 1962, 1994
PstI CTGCAG 2 cut(s) 838, 1531
PsuI RGATCY 4 cut(s) 287, 536, 775, 1652
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 6 cut(s) 607, 862, 888, 1700, 1971, 2118
RsaNI GTAC 6 cut(s) 606, 861, 887, 1699, 1970, 2117
SacI GAGCTC 1 cut(s) 493
Sau96I GGNCC 4 cut(s) 381, 848, 1355, 1605
ScrFI CCNGG 5 cut(s) 675, 1404, 1689, 1745, 1943
SduI GDGCHC 5 cut(s) 137, 493, 674, 1159, 1251
SfaNI GCATC 6 cut(s) 1015, 1042, 1108, 1239, 1741, 2075
SfcI CTRYAG 4 cut(s) 780, 834, 1527, 1596
SinI GGWCC 1 cut(s) 1355
SmlI CTYRAG 2 cut(s) 1250, 1974
SmoI CTYRAG 2 cut(s) 1250, 1974
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 2 cut(s) 825, 1520
SspMI CTAG 2 cut(s) 596, 1682
SstI GAGCTC 1 cut(s) 493
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 5 cut(s) 673, 1402, 1687, 1743, 1941
StyI CCWWGG 2 cut(s) 881, 1503
TaaI ACNGT 8 cut(s) 845, 865, 895, 1085, 1484, 1894, 2020, 2026
TaiI ACGT 2 cut(s) 1629, 1905
TaqI TCGA 6 cut(s) 117, 366, 663, 768, 1050, 1364
TaqII GACCGA 1 cut(s) 1372
TatI WGTACW 1 cut(s) 2116
TauI GCSGC 1 cut(s) 1523
TfiI GAWTC 2 cut(s) 386, 1946
TscAI CASTG 7 cut(s) 208, 376, 550, 850, 1090, 1189, 1866
TseFI GTSAC 3 cut(s) 757, 1861, 2020
Tsp45I GTSAC 3 cut(s) 757, 1861, 2020
TspDTI ATGAA 7 cut(s) 45, 248, 537, 788, 821, 1625, 2124
TspRI CASTG 7 cut(s) 208, 376, 550, 850, 1090, 1189, 1866
Van91I CCANNNNNTGG 1 cut(s) 432
VneI GTGCAC 1 cut(s) 1155
VpaK11BI GGWCC 1 cut(s) 1355
XagI CCTNNNNNAGG 1 cut(s) 1218
XapI RAATTY 7 cut(s) 328, 375, 460, 659, 967, 1360, 1640
XbaI TCTAGA 1 cut(s) 1681
XceI RCATGY 3 cut(s) 1280, 1475, 2060
XmiI GTMKAC 3 cut(s) 54, 112, 1486
XmnI GAANNNNTTC 2 cut(s) 980, 2012
XspI CTAG 2 cut(s) 596, 1682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.