MD11G1092000.v1.1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000257
Physical Location & Seq
Reverse (-)
7646580 .. 7650672
4093 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1092000.v1.1.491

Sequence Viewer

Length: 2112 bp
ATGGTGTTCACGACGTTGGAAGTTCATATCGTGTACTTGGGGTCTCTTCCTGACAGGGAGTTCTCGCCCTTGAATCACCACCATAGTATTCTGCAGAGAGTTGTTCAGACCAGTTCACCGGAAAATTTATTGGTACGAAGTTACAAAAGGAGTTTCAATGGGTTTGCTGCCAAACTCACTGACGAGGAAAGAGAGAAACTTGCTAGCATGAAAGAAGTTGTATCTGTCTTTCCAAGCACAACATTCCAACTGCACACGACGAGATCTTGGGACTTTATGGGTTTAGATGAGAAAACCAGTCGAAAGTCTCTGGTTGAGAGTGATATAATTGTGGGGGTTATTGACAGTGGAATATGGCCGGAATCTGAGAGCTTTAAAGATGAAGGTTTTGGTCCTCCTCCCAAGAGGTGGAAAGGTGCTTGTGAAGGTGGAAAAAATTTCACTTGCAACAACAAGCTCATTGGAGTTCGATTCTACGACACGGAGTCAGCAAGGGATGAAGACGGCCATGGAACTCACGTTGCCTCAATCGCAGCAGGCAATGCTGTTAAGGACGTAAGCTTTTATGGACTAGCAAATGGTACTGCAAGAGGAGGTGTTCCTTCTGCAAGAATAGCAGCATACAAAGTCTGTGCTAATACGTGCAGAATAGAAAGTATCTTGGCTGCCTTTGATGATGCTATTGCTGATGGAGTTGATGTCATTATGATTCAAATGGGAGCTGATTCGGGGATTCGATTTGATGAGGATCCTATGTTAATTGGTGCTTTTCATGCAATGAAGAAAGGGATACTGACCACACAGTCAGCAGGCAATGAAGGTCCCGGACCCGCTACTTCGTCAGATTTTGCACCATGGGTATTGCTCGTTGCAGCTAGTACCATAGATCGCCATATCATTGACAAGGTTGTTCTTGCTAACGGAAAGACACTTGTTGGGAATTCCGTCAACACTTTCACAACAAATGGAACAAGTTTTCCTCTAATATATGGAAAAGATGCTGCGACAAAAAATTGCACTCAACTAAATGCGGGACTCTGTCGTGAAGGTTGCCTGGACAGTGATTCTGTAAAGGGAAAGATAGTGGTATGCGATGCTGAATACGGAGATTATATGGCTTACGAAGCCAATGCATTTGGTTCACTTTTACATGAAAACGACAGAACAGCGTTTAATGTTCCCCTAATCCCTTCAACCGTGTTAATGGATGAAGACCATGATGCGGTCAAGTCCTACATGAACTCCACCAGAAACCCTCGGGCGAACATACTTAAAAGCGAAGTCATAAAAGACCCTTCTGCACCTATTGTTGCTTCCTACTCTTCACGTGGACCAAATCGATTTTTTCCTGATATTATTAAGCCAGATATTAGTGCACCGGGTTCTGAAATTTTGGCTGCATATTCACCTATTGCCTCTATCTCAGAATATGATGAAGACATGAGGCGTGTAAAATACAACTTGTTATCTGGAACCTCCATGTCTAGCCCACATGCAGCCGGTGCAGCCGCGTATGTTAAAGCATTCCACCCTGATTGGTCTCCGGCAGCCATCAAATCATCTCTTATGACAACTGCTTGGACCATGAATAGTACCAACAATTCTACCGGTGAATTTGCTTATGGATCTGGGCATATCAATCCTGTAGAAGCTGTAAACCCGGGGCTTGTGTATGAAGCTTCAGAAGAAGATTATATAAAGTTGTTGTGCACAGTGTTTGATGAAGGCAAAGTTAGACTCATATCAGGAGATAACAGCACGTGCCCTAAAGGTTCGGAAAAAGGGTATGCAAGGGATCTTAATTACCCTTCAATGGGAGCTAAAGTTGCAGAAATGGAGCCTTTTACAGTGAATTTTCATAGAAGAGTTAATAATGTTGGCCTCCTAAACTCTACTTACAGGGCCAGGATCTCCTCGAACTCCAAAGTTGATATCAAAGTGGTTCCTGAAGTTCTTTCGTTCGAGTCTTTGAACGAGGAGAGGACTTTTGATGTGATTGTCACTGGAAGCGGTTTGCCAGATCAATCTCAAGTGTCAGGATCGCTGGTTTGGACTGATGGTGTTCACAGCGTTAGAAGTCCAATTGTTGTTTATGTGTTCAATGGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

704

Amino Acids

76.22

Weight (kDa)

5.49

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 8 - 85 3.3e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 107 - 542 1.6e-40 Subtilase family
fn3_6 PF17766 599 - 697 3.2e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 802
AccB7I CCANNNNNTGG 1 cut(s) 408
AccII CGCG 1 cut(s) 1511
AciI CCGC 5 cut(s) 831, 1031, 1223, 1509, 2010
AclWI GGATC 6 cut(s) 743, 756, 1633, 1803, 1916, 2047
AcoI YGGCCR 2 cut(s) 356, 505
AcsI RAATTY 6 cut(s) 124, 436, 940, 1389, 1613, 1852
AcuI CTGAAG 2 cut(s) 1665, 1968
AcvI CACGTG 2 cut(s) 1328, 1761
AfaI GTAC 5 cut(s) 35, 135, 583, 880, 1594
AfiI CCNNNNNNNGG 4 cut(s) 408, 1222, 1813, 1814
AgeI ACCGGT 1 cut(s) 1607
AgsI TTSAA 7 cut(s) 73, 157, 713, 1194, 1812, 1972, 2101
AhdI GACNNNNNGTC 1 cut(s) 484
AjnI CCWGG 2 cut(s) 1053, 1904
AloI GAACNNNNNNTCC 2 cut(s) 961, 993
AluBI AGCT 8 cut(s) 372, 457, 561, 722, 875, 1652, 1679, 1820
AluI AGCT 8 cut(s) 372, 457, 561, 722, 875, 1652, 1679, 1820
Alw21I GWGCWC 2 cut(s) 1378, 1712
Alw26I GTCTC 3 cut(s) 48, 312, 1545
Alw44I GTGCAC 2 cut(s) 1374, 1708
AlwI GGATC 6 cut(s) 743, 756, 1633, 1803, 1916, 2047
Ama87I CYCGRG 2 cut(s) 1257, 1660
AoxI GGCC 4 cut(s) 356, 505, 1879, 1902
ApaLI GTGCAC 2 cut(s) 1374, 1708
ApoI RAATTY 6 cut(s) 124, 436, 940, 1389, 1613, 1852
AsiGI ACCGGT 1 cut(s) 1607
AspS9I GGNCC 6 cut(s) 392, 821, 827, 1331, 1581, 1902
AsuC2I CCSGG 4 cut(s) 825, 1380, 1661, 1662
AsuHPI GGTGA 4 cut(s) 68, 108, 1398, 1622
AsuNHI GCTAGC 1 cut(s) 203
AvaI CYCGRG 2 cut(s) 1257, 1660
AvaII GGWCC 5 cut(s) 392, 821, 827, 1331, 1581
BaeGI GKGCMC 3 cut(s) 1378, 1712, 1766
BamHI GGATCC 1 cut(s) 748
BbrPI CACGTG 2 cut(s) 1328, 1761
BbsI GAAGAC 3 cut(s) 507, 1218, 1443
Bbv12I GWGCWC 2 cut(s) 1378, 1712
BccI CCATC 3 cut(s) 683, 1559, 2051
BceAI ACGGC 1 cut(s) 520
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 2 cut(s) 1055, 1906
BciVI GTATCC 1 cut(s) 783
BcnI CCSGG 4 cut(s) 825, 1380, 1661, 1662
BcoDI GTCTC 3 cut(s) 48, 312, 1545
BfaI CTAG 4 cut(s) 204, 572, 876, 1485
BfmI CTRYAG 2 cut(s) 92, 1644
BfuI GTATCC 1 cut(s) 783
BglII AGATCT 1 cut(s) 263
Bme1390I CCNGG 6 cut(s) 825, 1055, 1380, 1661, 1662, 1906
Bme18I GGWCC 5 cut(s) 392, 821, 827, 1331, 1581
BmeRI GACNNNNNGTC 1 cut(s) 484
BmeT110I CYCGRG 2 cut(s) 1257, 1660
BmgT120I GGNCC 6 cut(s) 392, 821, 827, 1331, 1581, 1902
BmiI GGNNCC 6 cut(s) 750, 823, 829, 1474, 1839, 1944
BmrFI CCNGG 6 cut(s) 825, 1055, 1380, 1661, 1662, 1906
BmsI GCATC 4 cut(s) 667, 988, 1084, 1210
BmtI GCTAGC 1 cut(s) 207
BpiI GAAGAC 3 cut(s) 507, 1218, 1443
BpuEI CTTGAG 1 cut(s) 2013
BpuMI CCSGG 4 cut(s) 825, 1380, 1661, 1662
Bsa29I ATCGAT 1 cut(s) 1339
BsaAI YACGTR 3 cut(s) 642, 1328, 1761
BsaBI GATNNNNATC 1 cut(s) 747
BsaI GGTCTC 2 cut(s) 48, 1545
BsaJI CCNNGG 5 cut(s) 508, 854, 1256, 1660, 1661
BsaWI WCCGGW 2 cut(s) 118, 1607
BsaXI ACNNNNNCTCC 6 cut(s) 684, 714, 1226, 1256, 1809, 1839
Bsc4I CCNNNNNNNGG 4 cut(s) 408, 1222, 1813, 1814
Bse118I RCCGGY 2 cut(s) 1499, 1607
Bse1I ACTGG 3 cut(s) 111, 297, 2008
Bse3DI GCAATG 3 cut(s) 547, 783, 820
Bse8I GATNNNNATC 1 cut(s) 747
BseBI CCWGG 2 cut(s) 1055, 1906
BseCI ATCGAT 1 cut(s) 1339
BseDI CCNNGG 5 cut(s) 508, 854, 1256, 1660, 1661
BseGI GGATG 2 cut(s) 502, 1213
BseJI GATNNNNATC 1 cut(s) 747
BseLI CCNNNNNNNGG 4 cut(s) 408, 1222, 1813, 1814
BseMI GCAATG 3 cut(s) 547, 783, 820
BseMII CTCAG 2 cut(s) 357, 1437
BseNI ACTGG 3 cut(s) 111, 297, 2008
BseRI GAGGAG 4 cut(s) 387, 606, 1903, 1991
BseSI GKGCMC 3 cut(s) 1378, 1712, 1766
BsgI GTGCAG 4 cut(s) 236, 664, 1284, 1524
Bsh1236I CGCG 1 cut(s) 1511
BshFI GGCC 4 cut(s) 358, 507, 1881, 1904
BshTI ACCGGT 1 cut(s) 1607
BshVI ATCGAT 1 cut(s) 1339
BsiHKAI GWGCWC 2 cut(s) 1378, 1712
BsiHKCI CYCGRG 2 cut(s) 1257, 1660
BsiSI CCGG 8 cut(s) 119, 359, 825, 1379, 1500, 1544, 1608, 1661
BslFI GGGAC 3 cut(s) 284, 807, 1047
BslI CCNNNNNNNGG 4 cut(s) 408, 1222, 1813, 1814
BsmAI GTCTC 3 cut(s) 48, 312, 1545
BsmFI GGGAC 3 cut(s) 284, 807, 1047
BsmI GAATGC 1 cut(s) 1523
BsnI GGCC 4 cut(s) 358, 507, 1881, 1904
Bso31I GGTCTC 2 cut(s) 48, 1545
BsoBI CYCGRG 2 cut(s) 1257, 1660
Bsp1286I GDGCHC 3 cut(s) 1378, 1712, 1766
Bsp143I GATC 8 cut(s) 263, 748, 886, 1625, 1795, 1908, 2020, 2039
Bsp19I CCATGG 2 cut(s) 508, 854
BspACI CCGC 5 cut(s) 831, 1031, 1223, 1509, 2010
BspANI GGCC 4 cut(s) 358, 507, 1881, 1904
BspCNI CTCAG 2 cut(s) 358, 1436
BspDI ATCGAT 1 cut(s) 1339
BspFNI CGCG 1 cut(s) 1511
BspLI GGNNCC 6 cut(s) 750, 823, 829, 1474, 1839, 1944
BspMAI CTGCAG 1 cut(s) 96
BspOI GCTAGC 1 cut(s) 207
BspPI GGATC 6 cut(s) 743, 756, 1633, 1803, 1916, 2047
BspTNI GGTCTC 2 cut(s) 48, 1545
BsrDI GCAATG 3 cut(s) 547, 783, 820
BsrFI RCCGGY 2 cut(s) 1499, 1607
BsrI ACTGG 3 cut(s) 111, 297, 2008
BssAI RCCGGY 2 cut(s) 1499, 1607
BssECI CCNNGG 5 cut(s) 508, 854, 1256, 1660, 1661
BssMI GATC 8 cut(s) 263, 748, 886, 1625, 1795, 1908, 2020, 2039
BssT1I CCWWGG 2 cut(s) 508, 854
Bst2UI CCWGG 2 cut(s) 1055, 1906
Bst4CI ACNGT 6 cut(s) 347, 804, 1061, 1198, 1714, 1849
Bst6I CTCTTC 3 cut(s) 51, 1327, 1858
BstAPI GCANNNNNTGC 2 cut(s) 542, 1502
BstBAI YACGTR 3 cut(s) 642, 1328, 1761
BstC8I GCNNGC 3 cut(s) 205, 538, 811
BstDEI CTNAG 2 cut(s) 366, 1423
BstDSI CCRYGG 2 cut(s) 508, 854
BstF5I GGATG 2 cut(s) 502, 1213
BstFNI CGCG 1 cut(s) 1511
BstKTI GATC 8 cut(s) 266, 751, 889, 1628, 1798, 1911, 2023, 2042
BstMAI GTCTC 3 cut(s) 48, 312, 1545
BstMBI GATC 8 cut(s) 263, 748, 886, 1625, 1795, 1908, 2020, 2039
BstMWI GCNNNNNNNGC 8 cut(s) 530, 542, 614, 773, 1124, 1502, 1505, 1826
BstNI CCWGG 2 cut(s) 1055, 1906
BstNSI RCATGY 1 cut(s) 1496
BstSCI CCNGG 6 cut(s) 823, 1053, 1378, 1659, 1660, 1904
BstSFI CTRYAG 2 cut(s) 92, 1644
BstSLI GKGCMC 3 cut(s) 1378, 1712, 1766
BstUI CGCG 1 cut(s) 1511
BstV2I GAAGAC 3 cut(s) 507, 1218, 1443
BstX2I RGATCY 5 cut(s) 263, 748, 1625, 1795, 1908
BstYI RGATCY 5 cut(s) 263, 748, 1625, 1795, 1908
Bsu15I ATCGAT 1 cut(s) 1339
BsuI GTATCC 1 cut(s) 783
BsuRI GGCC 4 cut(s) 358, 507, 1881, 1904
BsuTUI ATCGAT 1 cut(s) 1339
BtgI CCRYGG 2 cut(s) 508, 854
BtgZI GCGATG 1 cut(s) 1107
BtsCI GGATG 2 cut(s) 502, 1213
BtsIMutI CAGTG 6 cut(s) 177, 352, 1066, 1719, 1854, 2001
Cac8I GCNNGC 3 cut(s) 205, 538, 811
Cfr10I RCCGGY 2 cut(s) 1499, 1607
Cfr13I GGNCC 6 cut(s) 392, 821, 827, 1331, 1581, 1902
Cfr9I CCCGGG 1 cut(s) 1660
ClaI ATCGAT 1 cut(s) 1339
Csp6I GTAC 5 cut(s) 34, 134, 582, 879, 1593
CspAI ACCGGT 1 cut(s) 1607
CspCI CAANNNNNGTGG 2 cut(s) 1517, 1552
CviQI GTAC 5 cut(s) 34, 134, 582, 879, 1593
DdeI CTNAG 2 cut(s) 366, 1423
DpnI GATC 8 cut(s) 265, 750, 888, 1627, 1797, 1910, 2022, 2041
DpnII GATC 8 cut(s) 263, 748, 886, 1625, 1795, 1908, 2020, 2039
DraI TTTAAA 1 cut(s) 376
DrdI GACNNNNNNGTC 1 cut(s) 802
DriI GACNNNNNGTC 1 cut(s) 484
DseDI GACNNNNNNGTC 1 cut(s) 802
EaeI YGGCCR 2 cut(s) 356, 505
Eam1104I CTCTTC 3 cut(s) 51, 1327, 1858
Eam1105I GACNNNNNGTC 1 cut(s) 484
EarI CTCTTC 3 cut(s) 51, 1327, 1858
Eco130I CCWWGG 2 cut(s) 508, 854
Eco31I GGTCTC 2 cut(s) 48, 1545
Eco32I GATATC 1 cut(s) 1933
Eco47I GGWCC 5 cut(s) 392, 821, 827, 1331, 1581
Eco57I CTGAAG 2 cut(s) 1665, 1968
Eco72I CACGTG 2 cut(s) 1328, 1761
Eco88I CYCGRG 2 cut(s) 1257, 1660
EcoO109I RGGNCCY 1 cut(s) 821
EcoRI GAATTC 1 cut(s) 940
EcoRII CCWGG 2 cut(s) 1053, 1904
EcoRV GATATC 1 cut(s) 1933
EcoT14I CCWWGG 2 cut(s) 508, 854
EcoT22I ATGCAT 1 cut(s) 1135
ErhI CCWWGG 2 cut(s) 508, 854
FaqI GGGAC 3 cut(s) 284, 807, 1047
FauI CCCGC 2 cut(s) 838, 1024
FokI GGATG 2 cut(s) 509, 1220
FspBI CTAG 4 cut(s) 204, 572, 876, 1485
HaeIII GGCC 4 cut(s) 358, 507, 1881, 1904
HapII CCGG 8 cut(s) 119, 359, 825, 1379, 1500, 1544, 1608, 1661
HincII GTYRAC 1 cut(s) 949
HindII GTYRAC 1 cut(s) 949
HindIII AAGCTT 2 cut(s) 559, 1677
HpaII CCGG 8 cut(s) 119, 359, 825, 1379, 1500, 1544, 1608, 1661
HphI GGTGA 4 cut(s) 68, 108, 1398, 1622
Hpy188I TCNGA 7 cut(s) 108, 367, 844, 1387, 1426, 1684, 1777
Hpy188III TCNNGA 8 cut(s) 10, 50, 1043, 1349, 1470, 1746, 1946, 2037
Hpy99I CGWCG 2 cut(s) 16, 262
HpyAV CCTTC 9 cut(s) 377, 419, 612, 812, 1040, 1200, 1305, 1718, 1818
HpyCH4III ACNGT 6 cut(s) 347, 804, 1061, 1198, 1714, 1849
HpyCH4IV ACGT 6 cut(s) 14, 519, 555, 641, 1327, 1760
HpyF10VI GCNNNNNNNGC 8 cut(s) 530, 542, 614, 773, 1124, 1502, 1505, 1826
HpyF3I CTNAG 2 cut(s) 366, 1423
HpySE526I ACGT 6 cut(s) 14, 519, 555, 641, 1327, 1760
Kzo9I GATC 8 cut(s) 263, 748, 886, 1625, 1795, 1908, 2020, 2039
LmnI GCTCC 3 cut(s) 719, 1817, 1837
LweI GCATC 4 cut(s) 667, 988, 1084, 1210
MaeI CTAG 4 cut(s) 204, 572, 876, 1485
MaeII ACGT 6 cut(s) 14, 519, 555, 641, 1327, 1760
MaeIII GTNAC 2 cut(s) 140, 1999
MalI GATC 8 cut(s) 265, 750, 888, 1627, 1797, 1910, 2022, 2041
MboI GATC 8 cut(s) 263, 748, 886, 1625, 1795, 1908, 2020, 2039
MboII GAAGA 9 cut(s) 38, 512, 793, 1223, 1314, 1448, 1697, 1700, 1875
MfeI CAATTG 1 cut(s) 2082
MflI RGATCY 5 cut(s) 263, 748, 1625, 1795, 1908
MhlI GDGCHC 3 cut(s) 1378, 1712, 1766
MlyI GAGTC 4 cut(s) 494, 1029, 1731, 1973
MmeI TCCRAC 1 cut(s) 271
Mph1103I ATGCAT 1 cut(s) 1135
MspI CCGG 8 cut(s) 119, 359, 825, 1379, 1500, 1544, 1608, 1661
MspR9I CCNGG 6 cut(s) 825, 1055, 1380, 1661, 1662, 1906
MunI CAATTG 1 cut(s) 2082
Mva1269I GAATGC 1 cut(s) 1523
MvaI CCWGG 2 cut(s) 1055, 1906
MvnI CGCG 1 cut(s) 1511
MwoI GCNNNNNNNGC 8 cut(s) 530, 542, 614, 773, 1124, 1502, 1505, 1826
NciI CCSGG 4 cut(s) 825, 1380, 1661, 1662
NcoI CCATGG 2 cut(s) 508, 854
NdeII GATC 8 cut(s) 263, 748, 886, 1625, 1795, 1908, 2020, 2039
NheI GCTAGC 1 cut(s) 203
NlaIV GGNNCC 6 cut(s) 750, 823, 829, 1474, 1839, 1944
NmuCI GTSAC 1 cut(s) 1999
NsiI ATGCAT 1 cut(s) 1135
NspI RCATGY 1 cut(s) 1496
PctI GAATGC 1 cut(s) 1523
PfeI GAWTC 7 cut(s) 73, 362, 471, 709, 725, 733, 1064
PflFI GACNNNGTC 1 cut(s) 1038
PflMI CCANNNNNTGG 1 cut(s) 408
PfoI TCCNGGA 1 cut(s) 823
PinAI ACCGGT 1 cut(s) 1607
PleI GAGTC 4 cut(s) 493, 1029, 1731, 1972
PmaCI CACGTG 2 cut(s) 1328, 1761
PmlI CACGTG 2 cut(s) 1328, 1761
PpsI GAGTC 4 cut(s) 493, 1029, 1731, 1972
Ppu21I YACGTR 3 cut(s) 642, 1328, 1761
PpuMI RGGWCCY 1 cut(s) 821
Psp5II RGGWCCY 1 cut(s) 821
Psp6I CCWGG 2 cut(s) 1053, 1904
PspCI CACGTG 2 cut(s) 1328, 1761
PspGI CCWGG 2 cut(s) 1053, 1904
PspN4I GGNNCC 6 cut(s) 750, 823, 829, 1474, 1839, 1944
PspPI GGNCC 6 cut(s) 392, 821, 827, 1331, 1581, 1902
PspPPI RGGWCCY 1 cut(s) 821
PstI CTGCAG 1 cut(s) 96
PsuI RGATCY 5 cut(s) 263, 748, 1625, 1795, 1908
PsyI GACNNNGTC 1 cut(s) 1038
RsaI GTAC 5 cut(s) 35, 135, 583, 880, 1594
RsaNI GTAC 5 cut(s) 34, 134, 582, 879, 1593
Sau3AI GATC 8 cut(s) 263, 748, 886, 1625, 1795, 1908, 2020, 2039
Sau96I GGNCC 6 cut(s) 392, 821, 827, 1331, 1581, 1902
SchI GAGTC 4 cut(s) 494, 1029, 1731, 1973
ScrFI CCNGG 6 cut(s) 825, 1055, 1380, 1661, 1662, 1906
SduI GDGCHC 3 cut(s) 1378, 1712, 1766
SfaNI GCATC 4 cut(s) 667, 988, 1084, 1210
SfcI CTRYAG 2 cut(s) 92, 1644
SinI GGWCC 5 cut(s) 392, 821, 827, 1331, 1581
SmaI CCCGGG 1 cut(s) 1662
SmlI CTYRAG 1 cut(s) 2028
SmoI CTYRAG 1 cut(s) 2028
SsiI CCGC 5 cut(s) 831, 1031, 1223, 1509, 2010
SspMI CTAG 4 cut(s) 204, 572, 876, 1485
StyD4I CCNGG 6 cut(s) 823, 1053, 1378, 1659, 1660, 1904
StyI CCWWGG 2 cut(s) 508, 854
TaaI ACNGT 6 cut(s) 347, 804, 1061, 1198, 1714, 1849
TaiI ACGT 6 cut(s) 17, 522, 558, 644, 1330, 1763
TaqI TCGA 6 cut(s) 301, 469, 736, 1339, 1916, 1962
TatI WGTACW 1 cut(s) 33
TauI GCSGC 1 cut(s) 1511
TfiI GAWTC 7 cut(s) 73, 362, 471, 709, 725, 733, 1064
TscAI CASTG 6 cut(s) 184, 352, 1066, 1719, 1854, 2008
TseFI GTSAC 1 cut(s) 1999
Tsp45I GTSAC 1 cut(s) 1999
TspGWI ACGGA 4 cut(s) 497, 934, 936, 1119
TspMI CCCGGG 1 cut(s) 1660
TspRI CASTG 6 cut(s) 184, 352, 1066, 1719, 1854, 2008
Tth111I GACNNNGTC 1 cut(s) 1038
Van91I CCANNNNNTGG 1 cut(s) 408
VneI GTGCAC 2 cut(s) 1374, 1708
VpaK11BI GGWCC 5 cut(s) 392, 821, 827, 1331, 1581
XapI RAATTY 6 cut(s) 124, 436, 940, 1389, 1613, 1852
XceI RCATGY 1 cut(s) 1496
XmaI CCCGGG 1 cut(s) 1660
XspI CTAG 4 cut(s) 204, 572, 876, 1485
Zsp2I ATGCAT 1 cut(s) 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.