subtilase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 108 |
| AarI | CACCTGC | 1 cut(s) | 1466 |
| Acc36I | ACCTGC | 2 cut(s) | 761, 1466 |
| AccI | GTMKAC | 1 cut(s) | 52 |
| AccII | CGCG | 1 cut(s) | 1299 |
| AciI | CCGC | 9 cut(s) | 359, 515, 588, 610, 849, 1299, 1484, 1536, 1551 |
| AclWI | GGATC | 3 cut(s) | 1609, 1773, 1802 |
| AcoI | YGGCCR | 2 cut(s) | 320, 961 |
| AcsI | RAATTY | 3 cut(s) | 401, 446, 1589 |
| AcuI | CTGAAG | 2 cut(s) | 1886, 2009 |
| AfaI | GTAC | 4 cut(s) | 26, 553, 853, 1868 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 800 |
| AflII | CTTAAG | 1 cut(s) | 1568 |
| AflIII | ACRYGT | 2 cut(s) | 969, 1739 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1733 |
| AjnI | CCWGG | 2 cut(s) | 1348, 1941 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1884, 1916 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 848, 880, 1098, 1130 |
| Alw21I | GWGCWC | 1 cut(s) | 1348 |
| Alw26I | GTCTC | 4 cut(s) | 256, 706, 1521, 1801 |
| Alw44I | GTGCAC | 1 cut(s) | 1344 |
| AlwI | GGATC | 3 cut(s) | 1609, 1773, 1802 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 163 |
| Ama87I | CYCGRG | 1 cut(s) | 1580 |
| AoxI | GGCC | 7 cut(s) | 320, 394, 475, 791, 961, 1301, 1606 |
| ApaLI | GTGCAC | 1 cut(s) | 1344 |
| ApeKI | GCWGC | 8 cut(s) | 80, 131, 503, 524, 612, 635, 842, 1367 |
| ApoI | RAATTY | 3 cut(s) | 401, 446, 1589 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 335, 367, 1127, 1159 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 982 |
| AspA2I | CCTAGG | 1 cut(s) | 1069 |
| AspLEI | GCGC | 1 cut(s) | 1142 |
| AspS9I | GGNCC | 4 cut(s) | 356, 791, 856, 1301 |
| AsuC2I | CCSGG | 1 cut(s) | 1638 |
| AsuHPI | GGTGA | 4 cut(s) | 21, 32, 1048, 1496 |
| AsuNHI | GCTAGC | 1 cut(s) | 527 |
| AvaI | CYCGRG | 1 cut(s) | 1580 |
| AvaII | GGWCC | 2 cut(s) | 356, 856 |
| AvrII | CCTAGG | 1 cut(s) | 1069 |
| AxyI | CCTNAGG | 2 cut(s) | 1410, 1723 |
| BaeGI | GKGCMC | 1 cut(s) | 1348 |
| BauI | CACGAG | 1 cut(s) | 558 |
| Bbv12I | GWGCWC | 1 cut(s) | 1348 |
| BbvI | GCAGC | 8 cut(s) | 67, 118, 490, 511, 599, 622, 854, 1354 |
| BccI | CCATC | 6 cut(s) | 51, 653, 1291, 1979, 1988, 2024 |
| BceAI | ACGGC | 3 cut(s) | 516, 783, 976 |
| BciT130I | CCWGG | 2 cut(s) | 1350, 1943 |
| BclI | TGATCA | 1 cut(s) | 796 |
| BcnI | CCSGG | 1 cut(s) | 1638 |
| BcoDI | GTCTC | 4 cut(s) | 256, 706, 1521, 1801 |
| BfaI | CTAG | 3 cut(s) | 432, 528, 1070 |
| BfmI | CTRYAG | 2 cut(s) | 723, 1215 |
| BfrI | CTTAAG | 1 cut(s) | 1568 |
| BfuAI | ACCTGC | 2 cut(s) | 761, 1466 |
| BlnI | CCTAGG | 1 cut(s) | 1069 |
| BmcAI | AGTACT | 1 cut(s) | 26 |
| Bme1390I | CCNGG | 3 cut(s) | 1350, 1638, 1943 |
| Bme18I | GGWCC | 2 cut(s) | 356, 856 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1733 |
| BmeT110I | CYCGRG | 1 cut(s) | 1580 |
| BmgT120I | GGNCC | 4 cut(s) | 356, 791, 856, 1301 |
| BmiI | GGNNCC | 4 cut(s) | 382, 484, 1099, 1152 |
| BmrFI | CCNGG | 3 cut(s) | 1350, 1638, 1943 |
| BmsI | GCATC | 2 cut(s) | 92, 637 |
| BmtI | GCTAGC | 1 cut(s) | 531 |
| BpmI | CTGGAG | 2 cut(s) | 1116, 1964 |
| BpuEI | CTTGAG | 2 cut(s) | 129, 531 |
| BpuMI | CCSGG | 1 cut(s) | 1638 |
| BsaAI | YACGTR | 1 cut(s) | 1866 |
| BsaBI | GATNNNNATC | 1 cut(s) | 683 |
| BsaI | GGTCTC | 1 cut(s) | 1521 |
| BsaJI | CCNNGG | 7 cut(s) | 478, 573, 824, 1069, 1248, 1521, 1636 |
| BsaWI | WCCGGW | 2 cut(s) | 1378, 1894 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1098, 1128, 2026, 2056 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 800 |
| Bse118I | RCCGGY | 2 cut(s) | 858, 998 |
| Bse1I | ACTGG | 1 cut(s) | 1517 |
| Bse21I | CCTNAGG | 2 cut(s) | 1410, 1723 |
| Bse3DI | GCAATG | 3 cut(s) | 651, 753, 1124 |
| Bse8I | GATNNNNATC | 1 cut(s) | 683 |
| BseBI | CCWGG | 2 cut(s) | 1350, 1943 |
| BseDI | CCNNGG | 7 cut(s) | 478, 573, 824, 1069, 1248, 1521, 1636 |
| BseGI | GGATG | 2 cut(s) | 834, 1999 |
| BseJI | GATNNNNATC | 1 cut(s) | 683 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 800 |
| BseMI | GCAATG | 3 cut(s) | 651, 753, 1124 |
| BseMII | CTCAG | 8 cut(s) | 6, 61, 321, 471, 702, 1401, 1737, 1919 |
| BseNI | ACTGG | 1 cut(s) | 1517 |
| BseRI | GAGGAG | 2 cut(s) | 1265, 1961 |
| BseSI | GKGCMC | 1 cut(s) | 1348 |
| BseXI | GCAGC | 8 cut(s) | 67, 118, 490, 511, 599, 622, 854, 1354 |
| BseYI | CCCAGC | 1 cut(s) | 779 |
| BsgI | GTGCAG | 1 cut(s) | 1707 |
| Bsh1236I | CGCG | 1 cut(s) | 1299 |
| Bsh1285I | CGRYCG | 1 cut(s) | 954 |
| BshFI | GGCC | 7 cut(s) | 322, 396, 477, 793, 963, 1303, 1608 |
| BsiEI | CGRYCG | 1 cut(s) | 954 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1348 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1580 |
| BsiSI | CCGG | 6 cut(s) | 323, 859, 999, 1379, 1638, 1895 |
| BslFI | GGGAC | 2 cut(s) | 248, 952 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 800 |
| BsmAI | GTCTC | 4 cut(s) | 256, 706, 1521, 1801 |
| BsmBI | CGTCTC | 2 cut(s) | 256, 706 |
| BsmFI | GGGAC | 2 cut(s) | 248, 952 |
| BsnI | GGCC | 7 cut(s) | 322, 396, 477, 793, 963, 1303, 1608 |
| Bso31I | GGTCTC | 1 cut(s) | 1521 |
| BsoBI | CYCGRG | 1 cut(s) | 1580 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1348 |
| Bsp143I | GATC | 8 cut(s) | 227, 678, 796, 951, 1047, 1601, 1765, 1807 |
| Bsp19I | CCATGG | 2 cut(s) | 478, 824 |
| BspACI | CCGC | 9 cut(s) | 359, 515, 588, 610, 849, 1299, 1484, 1536, 1551 |
| BspANI | GGCC | 7 cut(s) | 322, 396, 477, 793, 963, 1303, 1608 |
| BspCNI | CTCAG | 8 cut(s) | 7, 60, 322, 470, 701, 1402, 1736, 1920 |
| BspFNI | CGCG | 1 cut(s) | 1299 |
| BspHI | TCATGA | 1 cut(s) | 208 |
| BspLI | GGNNCC | 4 cut(s) | 382, 484, 1099, 1152 |
| BspMAI | CTGCAG | 1 cut(s) | 1219 |
| BspMI | ACCTGC | 2 cut(s) | 761, 1466 |
| BspOI | GCTAGC | 1 cut(s) | 531 |
| BspPI | GGATC | 3 cut(s) | 1609, 1773, 1802 |
| BspTI | CTTAAG | 1 cut(s) | 1568 |
| BspTNI | GGTCTC | 1 cut(s) | 1521 |
| BsrDI | GCAATG | 3 cut(s) | 651, 753, 1124 |
| BsrFI | RCCGGY | 2 cut(s) | 858, 998 |
| BsrI | ACTGG | 1 cut(s) | 1517 |
| BssAI | RCCGGY | 2 cut(s) | 858, 998 |
| BssECI | CCNNGG | 7 cut(s) | 478, 573, 824, 1069, 1248, 1521, 1636 |
| BssMI | GATC | 8 cut(s) | 227, 678, 796, 951, 1047, 1601, 1765, 1807 |
| BssNAI | GTATAC | 1 cut(s) | 53 |
| BssSI | CACGAG | 1 cut(s) | 558 |
| BssT1I | CCWWGG | 3 cut(s) | 478, 824, 1069 |
| Bst1107I | GTATAC | 1 cut(s) | 53 |
| Bst2BI | CACGAG | 1 cut(s) | 558 |
| Bst2UI | CCWGG | 2 cut(s) | 1350, 1943 |
| Bst4CI | ACNGT | 5 cut(s) | 311, 539, 838, 1837, 2042 |
| Bst6I | CTCTTC | 2 cut(s) | 1171, 1804 |
| BstAFI | CTTAAG | 1 cut(s) | 1568 |
| BstBAI | YACGTR | 1 cut(s) | 1866 |
| BstC8I | GCNNGC | 4 cut(s) | 409, 529, 847, 1301 |
| BstDSI | CCRYGG | 2 cut(s) | 478, 824 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 798 |
| BstF5I | GGATG | 2 cut(s) | 834, 1999 |
| BstFNI | CGCG | 1 cut(s) | 1299 |
| BstHHI | GCGC | 1 cut(s) | 1142 |
| BstKTI | GATC | 8 cut(s) | 230, 681, 799, 954, 1050, 1604, 1768, 1810 |
| BstMAI | GTCTC | 4 cut(s) | 256, 706, 1521, 1801 |
| BstMBI | GATC | 8 cut(s) | 227, 678, 796, 951, 1047, 1601, 1765, 1807 |
| BstMCI | CGRYCG | 1 cut(s) | 954 |
| BstNI | CCWGG | 2 cut(s) | 1350, 1943 |
| BstNSI | RCATGY | 1 cut(s) | 1743 |
| BstSCI | CCNGG | 3 cut(s) | 1348, 1636, 1941 |
| BstSFI | CTRYAG | 2 cut(s) | 723, 1215 |
| BstSLI | GKGCMC | 1 cut(s) | 1348 |
| BstSNI | TACGTA | 1 cut(s) | 1866 |
| BstUI | CGCG | 1 cut(s) | 1299 |
| BstV1I | GCAGC | 8 cut(s) | 67, 118, 490, 511, 599, 622, 854, 1354 |
| BstX2I | RGATCY | 1 cut(s) | 1765 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 372 |
| BstYI | RGATCY | 1 cut(s) | 1765 |
| BstZ17I | GTATAC | 1 cut(s) | 53 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1410, 1723 |
| BsuRI | GGCC | 7 cut(s) | 322, 396, 477, 793, 963, 1303, 1608 |
| BtgI | CCRYGG | 2 cut(s) | 478, 824 |
| BtgZI | GCGATG | 1 cut(s) | 69 |
| BtsCI | GGATG | 2 cut(s) | 834, 1999 |
| BtsI | GCAGTG | 1 cut(s) | 489 |
| BtsIMutI | CAGTG | 4 cut(s) | 195, 316, 489, 2047 |
| BveI | ACCTGC | 2 cut(s) | 761, 1466 |
| Cac8I | GCNNGC | 4 cut(s) | 409, 529, 847, 1301 |
| CaiI | CAGNNNCTG | 1 cut(s) | 163 |
| CciI | TCATGA | 1 cut(s) | 208 |
| CfoI | GCGC | 1 cut(s) | 1142 |
| Cfr10I | RCCGGY | 2 cut(s) | 858, 998 |
| Cfr13I | GGNCC | 4 cut(s) | 356, 791, 856, 1301 |
| CpoI | CGGWCCG | 1 cut(s) | 856 |
| CseI | GACGC | 1 cut(s) | 483 |
| Csp6I | GTAC | 4 cut(s) | 25, 552, 852, 1867 |
| CspI | CGGWCCG | 1 cut(s) | 856 |
| CviAII | CATG | 7 cut(s) | 209, 231, 479, 825, 1469, 1561, 1740 |
| CviQI | GTAC | 4 cut(s) | 25, 552, 852, 1867 |
| DpnI | GATC | 8 cut(s) | 229, 680, 798, 953, 1049, 1603, 1767, 1809 |
| DpnII | GATC | 8 cut(s) | 227, 678, 796, 951, 1047, 1601, 1765, 1807 |
| DraI | TTTAAA | 1 cut(s) | 1828 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1733 |
| EaeI | YGGCCR | 2 cut(s) | 320, 961 |
| Eam1104I | CTCTTC | 2 cut(s) | 1171, 1804 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1733 |
| EarI | CTCTTC | 2 cut(s) | 1171, 1804 |
| EciI | GGCGGA | 1 cut(s) | 348 |
| Eco105I | TACGTA | 1 cut(s) | 1866 |
| Eco130I | CCWWGG | 3 cut(s) | 478, 824, 1069 |
| Eco31I | GGTCTC | 1 cut(s) | 1521 |
| Eco47I | GGWCC | 2 cut(s) | 356, 856 |
| Eco57I | CTGAAG | 2 cut(s) | 1886, 2009 |
| Eco81I | CCTNAGG | 2 cut(s) | 1410, 1723 |
| Eco88I | CYCGRG | 1 cut(s) | 1580 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 798 |
| EcoRII | CCWGG | 2 cut(s) | 1348, 1941 |
| EcoT14I | CCWWGG | 3 cut(s) | 478, 824, 1069 |
| ErhI | CCWWGG | 3 cut(s) | 478, 824, 1069 |
| Esp3I | CGTCTC | 2 cut(s) | 256, 706 |
| FaeI | CATG | 7 cut(s) | 212, 234, 482, 828, 1472, 1564, 1743 |
| FaqI | GGGAC | 2 cut(s) | 248, 952 |
| FatI | CATG | 7 cut(s) | 208, 230, 478, 824, 1468, 1560, 1739 |
| FauI | CCCGC | 1 cut(s) | 1292 |
| FauNDI | CATATG | 2 cut(s) | 1276, 1597 |
| FbaI | TGATCA | 1 cut(s) | 796 |
| FblI | GTMKAC | 1 cut(s) | 52 |
| FokI | GGATG | 2 cut(s) | 841, 2006 |
| FspBI | CTAG | 3 cut(s) | 432, 528, 1070 |
| GlaI | GCGC | 1 cut(s) | 1141 |
| GsaI | CCCAGC | 1 cut(s) | 783 |
| GsuI | CTGGAG | 2 cut(s) | 1116, 1964 |
| HaeIII | GGCC | 7 cut(s) | 322, 396, 477, 793, 963, 1303, 1608 |
| HapII | CCGG | 6 cut(s) | 323, 859, 999, 1379, 1638, 1895 |
| HgaI | GACGC | 1 cut(s) | 483 |
| HhaI | GCGC | 1 cut(s) | 1142 |
| Hin1II | CATG | 7 cut(s) | 212, 234, 482, 828, 1472, 1564, 1743 |
| Hin6I | GCGC | 1 cut(s) | 1140 |
| HinP1I | GCGC | 1 cut(s) | 1140 |
| HincII | GTYRAC | 1 cut(s) | 498 |
| HindII | GTYRAC | 1 cut(s) | 498 |
| HindIII | AAGCTT | 1 cut(s) | 1083 |
| HpaII | CCGG | 6 cut(s) | 323, 859, 999, 1379, 1638, 1895 |
| HphI | GGTGA | 4 cut(s) | 21, 32, 1048, 1496 |
| Hpy166II | GTNNAC | 4 cut(s) | 53, 498, 958, 1346 |
| Hpy188III | TCNNGA | 9 cut(s) | 14, 209, 676, 949, 975, 1133, 1327, 1725, 1805 |
| Hpy8I | GTNNAC | 4 cut(s) | 53, 498, 958, 1346 |
| Hpy99I | CGWCG | 1 cut(s) | 347 |
| HpyAV | CCTTC | 4 cut(s) | 341, 467, 1401, 1984 |
| HpyCH4III | ACNGT | 5 cut(s) | 311, 539, 838, 1837, 2042 |
| HpyCH4IV | ACGT | 7 cut(s) | 699, 969, 1338, 1734, 1790, 1823, 1865 |
| HpySE526I | ACGT | 7 cut(s) | 699, 969, 1338, 1734, 1790, 1823, 1865 |
| Hsp92II | CATG | 7 cut(s) | 212, 234, 482, 828, 1472, 1564, 1743 |
| HspAI | GCGC | 1 cut(s) | 1140 |
| Ksp22I | TGATCA | 1 cut(s) | 796 |
| Kzo9I | GATC | 8 cut(s) | 227, 678, 796, 951, 1047, 1601, 1765, 1807 |
| LmnI | GCTCC | 5 cut(s) | 114, 428, 1097, 1276, 1897 |
| Lsp1109I | GCAGC | 8 cut(s) | 67, 118, 490, 511, 599, 622, 854, 1354 |
| LweI | GCATC | 2 cut(s) | 92, 637 |
| MaeI | CTAG | 3 cut(s) | 432, 528, 1070 |
| MaeII | ACGT | 7 cut(s) | 699, 969, 1338, 1734, 1790, 1823, 1865 |
| MaeIII | GTNAC | 8 cut(s) | 887, 1058, 1631, 1735, 1819, 1837, 1966, 2036 |
| MalI | GATC | 8 cut(s) | 229, 680, 798, 953, 1049, 1603, 1767, 1809 |
| MboI | GATC | 8 cut(s) | 227, 678, 796, 951, 1047, 1601, 1765, 1807 |
| MboII | GAAGA | 7 cut(s) | 1057, 1158, 1284, 1585, 1676, 1791, 1963 |
| MflI | RGATCY | 1 cut(s) | 1765 |
| MhlI | GDGCHC | 1 cut(s) | 1348 |
| MluCI | AATT | 7 cut(s) | 401, 446, 706, 979, 1589, 1611, 1771 |
| MlyI | GAGTC | 6 cut(s) | 163, 214, 449, 885, 1116, 2044 |
| MmeI | TCCRAC | 4 cut(s) | 223, 277, 1828, 1915 |
| MroXI | GAANNNNTTC | 1 cut(s) | 982 |
| MseI | TTAA | 8 cut(s) | 21, 713, 902, 918, 1569, 1614, 1827, 2080 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1454 |
| MspA1I | CMGCKG | 3 cut(s) | 612, 779, 849 |
| MspCI | CTTAAG | 1 cut(s) | 1568 |
| MspI | CCGG | 6 cut(s) | 323, 859, 999, 1379, 1638, 1895 |
| MspR9I | CCNGG | 3 cut(s) | 1350, 1638, 1943 |
| MvaI | CCWGG | 2 cut(s) | 1350, 1943 |
| MvnI | CGCG | 1 cut(s) | 1299 |
| NciI | CCSGG | 1 cut(s) | 1638 |
| NcoI | CCATGG | 2 cut(s) | 478, 824 |
| NdeI | CATATG | 2 cut(s) | 1276, 1597 |
| NdeII | GATC | 8 cut(s) | 227, 678, 796, 951, 1047, 1601, 1765, 1807 |
| NheI | GCTAGC | 1 cut(s) | 527 |
| NlaIII | CATG | 7 cut(s) | 212, 234, 482, 828, 1472, 1564, 1743 |
| NlaIV | GGNNCC | 4 cut(s) | 382, 484, 1099, 1152 |
| NmeAIII | GCCGAG | 3 cut(s) | 175, 554, 1502 |
| NmuCI | GTSAC | 7 cut(s) | 1058, 1631, 1735, 1819, 1837, 1966, 2036 |
| NspI | RCATGY | 1 cut(s) | 1743 |
| PagI | TCATGA | 1 cut(s) | 208 |
| PaqCI | CACCTGC | 1 cut(s) | 1466 |
| PciI | ACATGT | 1 cut(s) | 1739 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 249, 966 |
| PdmI | GAANNNNTTC | 1 cut(s) | 982 |
| PfeI | GAWTC | 9 cut(s) | 126, 315, 326, 759, 1211, 1360, 1700, 1851, 1932 |
| PflFI | GACNNNGTC | 2 cut(s) | 239, 875 |
| PfoI | TCCNGGA | 1 cut(s) | 1941 |
| Ple19I | CGATCG | 1 cut(s) | 954 |
| PleI | GAGTC | 6 cut(s) | 162, 213, 449, 884, 1115, 2043 |
| PpsI | GAGTC | 6 cut(s) | 162, 213, 449, 884, 1115, 2043 |
| Ppu21I | YACGTR | 1 cut(s) | 1866 |
| PscI | ACATGT | 1 cut(s) | 1739 |
| PsiI | TTATAA | 1 cut(s) | 108 |
| Psp6I | CCWGG | 2 cut(s) | 1348, 1941 |
| PspFI | CCCAGC | 1 cut(s) | 779 |
| PspGI | CCWGG | 2 cut(s) | 1348, 1941 |
| PspN4I | GGNNCC | 4 cut(s) | 382, 484, 1099, 1152 |
| PspPI | GGNCC | 4 cut(s) | 356, 791, 856, 1301 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1908, 1940 |
| PstI | CTGCAG | 1 cut(s) | 1219 |
| PstNI | CAGNNNCTG | 1 cut(s) | 163 |
| PsuI | RGATCY | 1 cut(s) | 1765 |
| PsyI | GACNNNGTC | 2 cut(s) | 239, 875 |
| PvuI | CGATCG | 1 cut(s) | 954 |
| PvuII | CAGCTG | 1 cut(s) | 779 |
| RsaI | GTAC | 4 cut(s) | 26, 553, 853, 1868 |
| RsaNI | GTAC | 4 cut(s) | 25, 552, 852, 1867 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1454 |
| Rsr2I | CGGWCCG | 1 cut(s) | 856 |
| RsrII | CGGWCCG | 1 cut(s) | 856 |
| SaqAI | TTAA | 8 cut(s) | 21, 713, 902, 918, 1569, 1614, 1827, 2080 |
| Sau3AI | GATC | 8 cut(s) | 227, 678, 796, 951, 1047, 1601, 1765, 1807 |
| Sau96I | GGNCC | 4 cut(s) | 356, 791, 856, 1301 |
| ScaI | AGTACT | 1 cut(s) | 26 |
| SchI | GAGTC | 6 cut(s) | 163, 214, 449, 885, 1116, 2044 |
| ScrFI | CCNGG | 3 cut(s) | 1350, 1638, 1943 |
| SduI | GDGCHC | 1 cut(s) | 1348 |
| SfaNI | GCATC | 2 cut(s) | 92, 637 |
| SfcI | CTRYAG | 2 cut(s) | 723, 1215 |
| SinI | GGWCC | 2 cut(s) | 356, 856 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1454 |
| SmlI | CTYRAG | 3 cut(s) | 144, 546, 1568 |
| SmoI | CTYRAG | 3 cut(s) | 144, 546, 1568 |
| SnaBI | TACGTA | 1 cut(s) | 1866 |
| Sse9I | AATT | 7 cut(s) | 401, 446, 706, 979, 1589, 1611, 1771 |
| SsiI | CCGC | 9 cut(s) | 359, 515, 588, 610, 849, 1299, 1484, 1536, 1551 |
| SspMI | CTAG | 3 cut(s) | 432, 528, 1070 |
| StyD4I | CCNGG | 3 cut(s) | 1348, 1636, 1941 |
| StyI | CCWWGG | 3 cut(s) | 478, 824, 1069 |
| TaaI | ACNGT | 5 cut(s) | 311, 539, 838, 1837, 2042 |
| TaiI | ACGT | 7 cut(s) | 702, 972, 1341, 1737, 1793, 1826, 1868 |
| TaqI | TCGA | 2 cut(s) | 677, 974 |
| TasI | AATT | 7 cut(s) | 401, 446, 706, 979, 1589, 1611, 1771 |
| TatI | WGTACW | 1 cut(s) | 24 |
| TauI | GCSGC | 2 cut(s) | 590, 1487 |
| TfiI | GAWTC | 9 cut(s) | 126, 315, 326, 759, 1211, 1360, 1700, 1851, 1932 |
| Tru1I | TTAA | 8 cut(s) | 21, 713, 902, 918, 1569, 1614, 1827, 2080 |
| Tru9I | TTAA | 8 cut(s) | 21, 713, 902, 918, 1569, 1614, 1827, 2080 |
| TscAI | CASTG | 4 cut(s) | 195, 316, 496, 2047 |
| TseFI | GTSAC | 7 cut(s) | 1058, 1631, 1735, 1819, 1837, 1966, 2036 |
| TseI | GCWGC | 8 cut(s) | 80, 131, 503, 524, 612, 635, 842, 1367 |
| Tsp45I | GTSAC | 7 cut(s) | 1058, 1631, 1735, 1819, 1837, 1966, 2036 |
| TspDTI | ATGAA | 5 cut(s) | 188, 225, 856, 1577, 1665 |
| TspGWI | ACGGA | 2 cut(s) | 869, 906 |
| TspRI | CASTG | 4 cut(s) | 195, 316, 496, 2047 |
| Tth111I | GACNNNGTC | 2 cut(s) | 239, 875 |
| Vha464I | CTTAAG | 1 cut(s) | 1568 |
| VneI | GTGCAC | 1 cut(s) | 1344 |
| VpaK11BI | GGWCC | 2 cut(s) | 356, 856 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 798 |
| XapI | RAATTY | 3 cut(s) | 401, 446, 1589 |
| XceI | RCATGY | 1 cut(s) | 1743 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 65 |
| XmaJI | CCTAGG | 1 cut(s) | 1069 |
| XmiI | GTMKAC | 1 cut(s) | 52 |
| XmnI | GAANNNNTTC | 1 cut(s) | 982 |
| XspI | CTAG | 3 cut(s) | 432, 528, 1070 |
| ZrmI | AGTACT | 1 cut(s) | 26 |