Rroxscaffold_4G00326580

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000257
Physical Location & Seq
Forward (+)
58752169 .. 58752887
719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326580.1

Sequence Viewer

Length: 669 bp
ATGGCAAAGCATAGAGCTCTCCAGGTTTCCTATGTTGTCCCCACTCTTATACTAACCTTGAGCTTCTTCTTCTGCAAAGCCATCGATGAAAACAGAAAGCTCCATATCGTGTACCTGGGGTCACTTCCTGATAAAAGAGTTGTCGAGAGCAACTCCACTTCCAATTTTCTTACAAGAAGTTACCAAAGGAGTTTCAATGGATTTGGTGCATACCTAACTGGCCGGGAAAGGGAAAGGCTTGCTAGCATGATAGAAGTAGTGTCTGTATTTCCAAGCACAACTTTCCAACTTCAAACGACAAGATCTTGGGACTTTCTCGGTTTCAATGAGAAAATCCAACGAAATGACAGCGTTGAGAGTGATATCATTATTGGTGTCATTGACTCTGGAATTTGGCCTGATTCGGAGAGCTTCAAAGACGATGGTTTTGGTCCTCCTCCCAAGAAGTGGAAAGGTGCTTGTGTAGGTGGCAAAAATTTTACTTGTAATAATAAGCTCATTGGTGCTCGGTTTTACAAATCATCCTCTCAGTCTGCAAGGGATGAAACTGGCCATGGAACACACACTGCCTCAACAGAAGCCGGGAATGCTGTAAAGGATGTCAGCTTTTATGGTATAGCCCAAGGCATTGCAAGAGGGGGAGTTCCCTCAGCGAGAGTTGCTGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

222

Amino Acids

24.42

Weight (kDa)

9.28

Isoelectric Point (pI)

39.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 47 - 98 3e-08 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 447
AcoI YGGCCR 2 cut(s) 220, 550
AcsI RAATTY 2 cut(s) 390, 475
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 229, 447
AgsI TTSAA 4 cut(s) 196, 293, 325, 415
AjnI CCWGG 2 cut(s) 21, 114
AluBI AGCT 6 cut(s) 17, 63, 100, 411, 496, 606
AluI AGCT 6 cut(s) 17, 63, 100, 411, 496, 606
Alw21I GWGCWC 2 cut(s) 19, 508
AoxI GGCC 3 cut(s) 220, 395, 550
ApeKI GCWGC 1 cut(s) 662
ApoI RAATTY 2 cut(s) 390, 475
ArsI GACNNNNNNTTYG 2 cut(s) 410, 442
AspS9I GGNCC 1 cut(s) 431
AsuC2I CCSGG 2 cut(s) 224, 583
AsuNHI GCTAGC 1 cut(s) 242
AvaII GGWCC 1 cut(s) 431
BalI TGGCCA 1 cut(s) 552
BanII GRGCYC 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 19, 508
BbvCI CCTCAGC 1 cut(s) 649
BbvI GCAGC 1 cut(s) 649
BccI CCATC 2 cut(s) 89, 416
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BciT130I CCWGG 2 cut(s) 23, 116
BcnI CCSGG 2 cut(s) 224, 583
BfaI CTAG 1 cut(s) 243
BglII AGATCT 1 cut(s) 302
BisI GCNGC 1 cut(s) 663
BlsI GCNGC 1 cut(s) 664
Bme1390I CCNGG 4 cut(s) 23, 116, 224, 583
Bme18I GGWCC 1 cut(s) 431
BmgT120I GGNCC 1 cut(s) 431
BmrFI CCNGG 4 cut(s) 23, 116, 224, 583
BmtI GCTAGC 1 cut(s) 246
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpmI CTGGAG 1 cut(s) 5
Bpu10I CCTNAGC 1 cut(s) 649
BpuEI CTTGAG 1 cut(s) 79
BpuMI CCSGG 2 cut(s) 224, 583
Bsa29I ATCGAT 1 cut(s) 84
BsaJI CCNNGG 3 cut(s) 115, 553, 622
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 447
Bse1I ACTGG 2 cut(s) 223, 553
Bse3DI GCAATG 1 cut(s) 627
BseBI CCWGG 2 cut(s) 23, 116
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 3 cut(s) 115, 553, 622
BseGI GGATG 3 cut(s) 521, 547, 604
BseLI CCNNNNNNNGG 2 cut(s) 229, 447
BseMI GCAATG 1 cut(s) 627
BseMII CTCAG 2 cut(s) 542, 663
BseNI ACTGG 2 cut(s) 223, 553
BseRI GAGGAG 1 cut(s) 426
BseXI GCAGC 1 cut(s) 649
BshFI GGCC 3 cut(s) 222, 397, 552
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 2 cut(s) 19, 508
BsiSI CCGG 2 cut(s) 223, 582
BslFI GGGAC 2 cut(s) 23, 323
BslI CCNNNNNNNGG 2 cut(s) 229, 447
BsmFI GGGAC 2 cut(s) 23, 323
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 3 cut(s) 222, 397, 552
Bsp1286I GDGCHC 2 cut(s) 19, 508
Bsp143I GATC 1 cut(s) 302
Bsp19I CCATGG 1 cut(s) 553
BspANI GGCC 3 cut(s) 222, 397, 552
BspCNI CTCAG 2 cut(s) 541, 662
BspDI ATCGAT 1 cut(s) 84
BspOI GCTAGC 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 627
BsrI ACTGG 2 cut(s) 223, 553
BssECI CCNNGG 3 cut(s) 115, 553, 622
BssMI GATC 1 cut(s) 302
BssT1I CCWWGG 2 cut(s) 553, 622
Bst2UI CCWGG 2 cut(s) 23, 116
BstC8I GCNNGC 2 cut(s) 240, 244
BstDEI CTNAG 2 cut(s) 528, 649
BstDSI CCRYGG 1 cut(s) 553
BstF5I GGATG 3 cut(s) 521, 547, 604
BstKTI GATC 1 cut(s) 305
BstMBI GATC 1 cut(s) 302
BstMWI GCNNNNNNNGC 2 cut(s) 587, 659
BstNI CCWGG 2 cut(s) 23, 116
BstSCI CCNGG 4 cut(s) 21, 114, 222, 581
BstV1I GCAGC 1 cut(s) 649
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
Bsu15I ATCGAT 1 cut(s) 84
BsuRI GGCC 3 cut(s) 222, 397, 552
BsuTUI ATCGAT 1 cut(s) 84
BtgI CCRYGG 1 cut(s) 553
BtsCI GGATG 3 cut(s) 521, 547, 604
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 1 cut(s) 564
Cac8I GCNNGC 2 cut(s) 240, 244
Cfr13I GGNCC 1 cut(s) 431
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 1 cut(s) 112
CviAII CATG 2 cut(s) 247, 554
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 2 cut(s) 528, 649
DpnI GATC 1 cut(s) 304
DpnII GATC 1 cut(s) 302
EaeI YGGCCR 2 cut(s) 220, 550
Ecl136II GAGCTC 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 553, 622
Eco24I GRGCYC 1 cut(s) 19
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 431
Eco53kI GAGCTC 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 21, 114
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 2 cut(s) 553, 622
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 2 cut(s) 553, 622
FaeI CATG 2 cut(s) 250, 557
FaiI YATR 9 cut(s) 12, 33, 50, 105, 211, 248, 555, 612, 617
FalI AAGNNNNNCTT 2 cut(s) 265, 297
FaqI GGGAC 2 cut(s) 23, 323
FatI CATG 2 cut(s) 246, 553
Fnu4HI GCNGC 1 cut(s) 663
FokI GGATG 3 cut(s) 508, 554, 611
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 1 cut(s) 663
FspBI CTAG 1 cut(s) 243
GluI GCNGC 1 cut(s) 663
GsuI CTGGAG 1 cut(s) 5
HaeIII GGCC 3 cut(s) 222, 397, 552
HapII CCGG 2 cut(s) 223, 582
Hin1II CATG 2 cut(s) 250, 557
HinfI GANTC 2 cut(s) 383, 401
HpaII CCGG 2 cut(s) 223, 582
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 1 cut(s) 406
Hpy188III TCNNGA 3 cut(s) 128, 145, 387
Hpy8I GTNNAC 1 cut(s) 112
HpyCH4V TGCA 4 cut(s) 75, 209, 536, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 587, 659
HpyF3I CTNAG 2 cut(s) 528, 649
Hsp92II CATG 2 cut(s) 250, 557
Kzo9I GATC 1 cut(s) 302
LmnI GCTCC 1 cut(s) 105
Lsp1109I GCAGC 1 cut(s) 649
MaeI CTAG 1 cut(s) 243
MaeIII GTNAC 2 cut(s) 120, 179
MalI GATC 1 cut(s) 304
MboI GATC 1 cut(s) 302
MboII GAAGA 2 cut(s) 58, 61
MflI RGATCY 1 cut(s) 302
MhlI GDGCHC 2 cut(s) 19, 508
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 3 cut(s) 163, 390, 475
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 1 cut(s) 377
MmeI TCCRAC 2 cut(s) 310, 361
MnlI CCTC 6 cut(s) 444, 447, 535, 580, 629, 658
Mox20I TGGCCA 1 cut(s) 552
MscI TGGCCA 1 cut(s) 552
Msp20I TGGCCA 1 cut(s) 552
MspI CCGG 2 cut(s) 223, 582
MspR9I CCNGG 4 cut(s) 23, 116, 224, 583
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 2 cut(s) 23, 116
MwoI GCNNNNNNNGC 2 cut(s) 587, 659
NciI CCSGG 2 cut(s) 224, 583
NcoI CCATGG 1 cut(s) 553
NdeII GATC 1 cut(s) 302
NheI GCTAGC 1 cut(s) 242
NlaIII CATG 2 cut(s) 250, 557
NmuCI GTSAC 1 cut(s) 120
PctI GAATGC 1 cut(s) 592
PfeI GAWTC 1 cut(s) 401
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 1 cut(s) 664
PleI GAGTC 1 cut(s) 377
PpsI GAGTC 1 cut(s) 377
Psp124BI GAGCTC 1 cut(s) 19
Psp6I CCWGG 2 cut(s) 21, 114
PspGI CCWGG 2 cut(s) 21, 114
PspPI GGNCC 1 cut(s) 431
PsuI RGATCY 1 cut(s) 302
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SacI GAGCTC 1 cut(s) 19
SatI GCNGC 1 cut(s) 663
Sau3AI GATC 1 cut(s) 302
Sau96I GGNCC 1 cut(s) 431
SchI GAGTC 1 cut(s) 377
ScrFI CCNGG 4 cut(s) 23, 116, 224, 583
SduI GDGCHC 2 cut(s) 19, 508
SinI GGWCC 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 58
SmoI CTYRAG 1 cut(s) 58
Sse9I AATT 3 cut(s) 163, 390, 475
SspMI CTAG 1 cut(s) 243
SstI GAGCTC 1 cut(s) 19
StyD4I CCNGG 4 cut(s) 21, 114, 222, 581
StyI CCWWGG 2 cut(s) 553, 622
TaqI TCGA 2 cut(s) 84, 144
TasI AATT 3 cut(s) 163, 390, 475
TfiI GAWTC 1 cut(s) 401
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 1 cut(s) 120
TseI GCWGC 1 cut(s) 662
Tsp45I GTSAC 1 cut(s) 120
TspDTI ATGAA 2 cut(s) 102, 558
TspRI CASTG 1 cut(s) 571
Van91I CCANNNNNTGG 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 431
XapI RAATTY 2 cut(s) 390, 475
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.