FvH4_7g02840

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000257
Physical Location & Seq
Reverse (-)
3167223 .. 3169640
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02840.t1

Sequence Viewer

Length: 2214 bp
ATGGCCAAGAATGGGGTTCTCGTGTTCTCTTATATTTTCCCAATTCTCATGCTCAATATGAGCTTGTTAGTTTGCATAAGCGCCATTGATGAAAACAGAAAGGTCTATATAGTATACATGGGCTCACTCCCCTCTAACGAGTCGTACTCGCCCTTGTCTCACCACCTTAATATATTAGAAAGAGTTGTCAAGAATGGTTCCGCTGCAAATCTCTTGATGAGAAGTTACAGAAGGAGTTTCAATGGATTTGCTGCCAAGCTCACTGACAATGAAAGGCAAGCGCTTGCTAACATGAAGGAAGTAGTGTCTGTCTTTCCAAGCACAACTCTCCAGCTTCAAACAACAAGATCCTGGGACTTCATAGGTCTGAACGAAACAACTCATCGAAATGCTACAGTCGAGAGTGATATCATTATTGGTGTCATTGACACAGGAATATGGCCTGAATCGGAGAGCTTTAAAGATGACGGATTTGCTCCGGCTCCAAAGAAGTGGAAAGGTGCGTGTAAAGGTGGCGAAAACTTCACATGCAACAACAAGATCATTGGAGCTCGGTTTTACGGACCAATACAGTCTGCAAGGGATGAAGAAGGCCATGGAACTCACACTGCCTCAACGGCAGCAGGAAATGACGTGAAGGCTGTGAGCTTTTATAAACTAGCACAAGGCGCAGCAAGAGGCGCAGTTCCCTCTGCGAGAATTGCTACGTATGCAGTCTGCAGTGCTAATCTAGGATGCTCTACAGAAAGTGTCTTGGCTGCTTTTGACGATGCTATTGCTGACGGTGTTGACGTCATTACAATTTCACTTGGAGGAATGTTTGCAATGCCTTTCCAGGAAGATGTGATCGCAATCGGTGCTTTCCATGCATCGGTGAAAGGGATACTAACCACAAACTCTGCAGGCAACAGTGGTCCTATCGCTGGTTCTGTTACAAGCGTGGCACCATGGTTGCTCTCAGTGGCCGCTAGTAGCATAGATCGTCGAATTATTGACAAGGTCATTCTTGGGAATGGAACCACAGTTGTTGGGCTATCAGTGAATTCTTTCACATTAAATGGAACAGGTTTCCCATTGATCTATGGAAAAGCTGCAGCTTCAAGGAACTGCTCGGAGTTCGCTGCGGGGGGATGTCACTCAGGCTGCCTGGATGGTGATTTAGTTAAGGGAAAGATTGTCTTGTGTGATGAAATCTCTGGACATGATGTCGCTTATGAAGCTGGAGCAGTAGGTTCAATTTTAAACGCTGATTGGGTTGATTTTCCTGTCATTCTAGCTCAACCTGCAACAATATTGAGAGATCATGAGTATAATGCAATCAAGTCCTACATGAATTCCACTGAAGATGCTCGAGCAAACATATTAAAAAGTGAAGCCGAAAGAGATGATACTGCGCCTGTTATTGCTTCCTTCTCTGCACGAGGACCAAATATAATACTACCTGAAATAATCAAGCCAGACATAAGTGCTCCAGGGGTTACTATTTTGGCTGCCTATTCACCTGTTGCTCCAGTTACAAACAGTCCTGAAGACAAGAGGCATGTAAAGTACAGTATAATGTCTGGAACCTCCATGTCTTGTCCGCATGCGGCTGGTGCAGCTGCATATGTCAAAGCATTCCACCCTGACTGGTCTCCAGCAGCCATCAAATCATCTCTTATGACTACAGCTTGGCCTATGAATGTTACTAACGACTCCCCTGGTGAATTTGCCTATGGTTCTGGACATATCAATCCTGTCCAAGCTATAAACCCAGGACTGGTTTATGAAGCTTCTAAGGAAGATTACATAAAGCTCCTCTGCTCAATATTGGGGGAGAGCATGGTTAGACTTATATCAGGAGATAACAGCACATGCCCTACACACTCGGACAAAGACTCGCCAAAGGATCACAATTACCCATCATTTGCAGGTGTTGTTCCAGTAAAGAAAGCTTTCAGTCTGCAATTTCACAGAGTTGTTAAAAATGTTGGCTTTGCAAACTCCACTTACAGTGCCAAGATCTTTGGAAAAAACACTCAAGTGGATATCAAAGTGGTGCCTGAAGTTCTTTCCTTCGAGTCCTTGAATCAGGAAAAACCATTTGATGTCACCCTTGTTGGACCAGGATTGTCGGATGATTCACAACCACATGTGTCTGCGTCGCTCGTATGGTCTGATGGAACTCATAGTGTTAGAAGTCCAATTCTCATCCATAGCCAAAAAGAATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

738

Amino Acids

78.55

Weight (kDa)

5.96

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 113 3.4e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 567 1.3e-40 Subtilase family
fn3_6 PF17766 630 - 731 3.1e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 654
AarI CACCTGC 1 cut(s) 1901
AatII GACGTC 1 cut(s) 795
Acc36I ACCTGC 2 cut(s) 1291, 1901
AccB1I GGYRCC 2 cut(s) 943, 2038
AccI GTMKAC 1 cut(s) 114
AciI CCGC 5 cut(s) 201, 966, 1124, 1583, 1589
AclWI GGATC 2 cut(s) 342, 1896
AcoI YGGCCR 2 cut(s) 3, 963
AcsI RAATTY 3 cut(s) 1042, 1333, 1706
AcuI CTGAAG 3 cut(s) 1362, 1548, 2064
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 2 cut(s) 146, 1550
AfeI AGCGCT 1 cut(s) 282
AfiI CCNNNNNNNGG 4 cut(s) 12, 871, 923, 1759
AflIII ACRYGT 1 cut(s) 2131
AgsI TTSAA 5 cut(s) 241, 338, 1101, 1236, 2068
AhdI GACNNNNNGTC 1 cut(s) 1205
AjiI CACGTC 1 cut(s) 634
AjnI CCWGG 7 cut(s) 350, 834, 1146, 1471, 1699, 1753, 2104
AleI CACNNNNGTG 1 cut(s) 2021
Alw21I GWGCWC 2 cut(s) 553, 1471
Alw26I GTCTC 2 cut(s) 162, 1638
AlwI GGATC 2 cut(s) 342, 1896
Ama87I CYCGRG 1 cut(s) 1350
Aor51HI AGCGCT 1 cut(s) 282
AoxI GGCC 5 cut(s) 3, 440, 592, 963, 1673
ApoI RAATTY 3 cut(s) 1042, 1333, 1706
Asp700I GAANNNNTTC 2 cut(s) 1046, 1934
AspLEI GCGC 5 cut(s) 83, 283, 671, 683, 1396
AspS9I GGNCC 4 cut(s) 563, 914, 1424, 2102
AsuHPI GGTGA 6 cut(s) 152, 886, 1166, 1491, 1715, 2083
AvaI CYCGRG 1 cut(s) 1350
AvaII GGWCC 4 cut(s) 563, 914, 1424, 2102
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 943, 2038
BanII GRGCYC 2 cut(s) 125, 553
BauI CACGAG 2 cut(s) 20, 1419
BbsI GAAGAC 1 cut(s) 1536
Bbv12I GWGCWC 2 cut(s) 553, 1471
BccI CCATC 4 cut(s) 1145, 1652, 1909, 2153
BceAI ACGGC 1 cut(s) 633
BcgI CGANNNNNNTGC 2 cut(s) 758, 792
BciT130I CCWGG 7 cut(s) 352, 836, 1148, 1473, 1701, 1755, 2106
BciVI GTATCC 1 cut(s) 876
BcoDI GTCTC 2 cut(s) 162, 1638
BfaI CTAG 4 cut(s) 659, 731, 969, 1274
BfmI CTRYAG 6 cut(s) 393, 718, 741, 900, 1092, 1665
BfoI RGCGCY 2 cut(s) 84, 284
BfuAI ACCTGC 2 cut(s) 1291, 1901
BfuI GTATCC 1 cut(s) 876
BglI GCCNNNNNGGC 1 cut(s) 617
BglII AGATCT 1 cut(s) 2001
Bme1390I CCNGG 7 cut(s) 352, 836, 1148, 1473, 1701, 1755, 2106
Bme18I GGWCC 4 cut(s) 563, 914, 1424, 2102
BmeRI GACNNNNNGTC 1 cut(s) 1205
BmeT110I CYCGRG 1 cut(s) 1350
BmgBI CACGTC 1 cut(s) 634
BmgT120I GGNCC 4 cut(s) 563, 914, 1424, 2102
BmiI GGNNCC 6 cut(s) 199, 483, 945, 1018, 1567, 2040
BmrFI CCNGG 7 cut(s) 352, 836, 1148, 1473, 1701, 1755, 2106
BmsI GCATC 4 cut(s) 725, 760, 878, 1336
BpiI GAAGAC 1 cut(s) 1536
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 5 cut(s) 314, 1242, 1455, 1494, 1620
BpuEI CTTGAG 1 cut(s) 2004
BsaAI YACGTR 1 cut(s) 708
BsaBI GATNNNNATC 1 cut(s) 851
BsaHI GRCGYC 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 1638
BsaJI CCNNGG 6 cut(s) 351, 595, 947, 1472, 1699, 1753
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 871, 923, 1759
Bse1I ACTGG 4 cut(s) 1511, 1634, 1764, 1922
Bse3DI GCAATG 1 cut(s) 831
Bse8I GATNNNNATC 1 cut(s) 851
BseBI CCWGG 7 cut(s) 352, 836, 1148, 1473, 1701, 1755, 2106
BseDI CCNNGG 6 cut(s) 351, 595, 947, 1472, 1699, 1753
BseGI GGATG 6 cut(s) 589, 740, 1136, 1156, 2122, 2190
BseJI GATNNNNATC 1 cut(s) 851
BseLI CCNNNNNNNGG 4 cut(s) 12, 871, 923, 1759
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 2 cut(s) 972, 1152
BseNI ACTGG 4 cut(s) 1511, 1634, 1764, 1922
BseRI GAGGAG 1 cut(s) 1787
BsgI GTGCAG 2 cut(s) 1401, 1617
BshFI GGCC 5 cut(s) 5, 442, 594, 965, 1675
BshNI GGYRCC 2 cut(s) 943, 2038
BsiHKAI GWGCWC 2 cut(s) 553, 1471
BsiHKCI CYCGRG 1 cut(s) 1350
BsiSI CCGG 1 cut(s) 479
BslFI GGGAC 1 cut(s) 368
BslI CCNNNNNNNGG 4 cut(s) 12, 871, 923, 1759
BsmAI GTCTC 2 cut(s) 162, 1638
BsmFI GGGAC 1 cut(s) 368
BsmI GAATGC 1 cut(s) 1616
BsnI GGCC 5 cut(s) 5, 442, 594, 965, 1675
Bso31I GGTCTC 1 cut(s) 1638
BsoBI CYCGRG 1 cut(s) 1350
Bsp1286I GDGCHC 3 cut(s) 125, 553, 1471
Bsp143I GATC 8 cut(s) 347, 540, 846, 979, 1077, 1300, 1888, 2001
Bsp19I CCATGG 2 cut(s) 595, 947
BspACI CCGC 5 cut(s) 201, 966, 1124, 1583, 1589
BspANI GGCC 5 cut(s) 5, 442, 594, 965, 1675
BspCNI CTCAG 2 cut(s) 971, 1151
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 6 cut(s) 199, 483, 945, 1018, 1567, 2040
BspMAI CTGCAG 3 cut(s) 722, 904, 1096
BspMI ACCTGC 2 cut(s) 1291, 1901
BspPI GGATC 2 cut(s) 342, 1896
BspT107I GGYRCC 2 cut(s) 943, 2038
BspTNI GGTCTC 1 cut(s) 1638
BsrDI GCAATG 1 cut(s) 831
BsrI ACTGG 4 cut(s) 1511, 1634, 1764, 1922
BssECI CCNNGG 6 cut(s) 351, 595, 947, 1472, 1699, 1753
BssMI GATC 8 cut(s) 347, 540, 846, 979, 1077, 1300, 1888, 2001
BssNAI GTATAC 1 cut(s) 115
BssNI GRCGYC 1 cut(s) 792
BssSI CACGAG 2 cut(s) 20, 1419
BssT1I CCWWGG 2 cut(s) 595, 947
Bst1107I GTATAC 1 cut(s) 115
Bst2BI CACGAG 2 cut(s) 20, 1419
Bst2UI CCWGG 7 cut(s) 352, 836, 1148, 1473, 1701, 1755, 2106
Bst4CI ACNGT 8 cut(s) 397, 573, 785, 911, 1024, 1523, 1553, 1994
BstACI GRCGYC 1 cut(s) 792
BstAPI GCANNNNNTGC 1 cut(s) 857
BstBAI YACGTR 1 cut(s) 708
BstC8I GCNNGC 4 cut(s) 279, 285, 904, 1587
BstDEI CTNAG 3 cut(s) 958, 1138, 1776
BstDSI CCRYGG 2 cut(s) 595, 947
BstF5I GGATG 6 cut(s) 589, 740, 1136, 1156, 2122, 2190
BstH2I RGCGCY 2 cut(s) 84, 284
BstHHI GCGC 5 cut(s) 83, 283, 671, 683, 1396
BstKTI GATC 8 cut(s) 350, 543, 849, 982, 1080, 1303, 1891, 2004
BstMAI GTCTC 2 cut(s) 162, 1638
BstMBI GATC 8 cut(s) 347, 540, 846, 979, 1077, 1300, 1888, 2001
BstNI CCWGG 7 cut(s) 352, 836, 1148, 1473, 1701, 1755, 2106
BstNSI RCATGY 5 cut(s) 531, 1544, 1589, 1857, 2135
BstSCI CCNGG 7 cut(s) 350, 834, 1146, 1471, 1699, 1753, 2104
BstSFI CTRYAG 6 cut(s) 393, 718, 741, 900, 1092, 1665
BstSNI TACGTA 1 cut(s) 708
BstV2I GAAGAC 1 cut(s) 1536
BstX2I RGATCY 2 cut(s) 347, 2001
BstXI CCANNNNNNTGG 1 cut(s) 492
BstYI RGATCY 2 cut(s) 347, 2001
BstZ17I GTATAC 1 cut(s) 115
BsuI GTATCC 1 cut(s) 876
BsuRI GGCC 5 cut(s) 5, 442, 594, 965, 1675
BtgI CCRYGG 2 cut(s) 595, 947
BtrI CACGTC 1 cut(s) 634
BtsCI GGATG 6 cut(s) 589, 740, 1136, 1156, 2122, 2190
BtsI GCAGTG 2 cut(s) 606, 727
BtsIMutI CAGTG 8 cut(s) 261, 606, 727, 916, 966, 1044, 1338, 1999
BveI ACCTGC 2 cut(s) 1291, 1901
Cac8I GCNNGC 4 cut(s) 279, 285, 904, 1587
CciI TCATGA 1 cut(s) 1303
CfoI GCGC 5 cut(s) 83, 283, 671, 683, 1396
Cfr13I GGNCC 4 cut(s) 563, 914, 1424, 2102
CseI GACGC 1 cut(s) 2130
Csp6I GTAC 2 cut(s) 145, 1549
CspCI CAANNNNNGTGG 2 cut(s) 1009, 1044
CviQI GTAC 2 cut(s) 145, 1549
DdeI CTNAG 3 cut(s) 958, 1138, 1776
DpnI GATC 8 cut(s) 349, 542, 848, 981, 1079, 1302, 1890, 2003
DpnII GATC 8 cut(s) 347, 540, 846, 979, 1077, 1300, 1888, 2001
DraI TTTAAA 2 cut(s) 460, 1242
DriI GACNNNNNGTC 1 cut(s) 1205
EaeI YGGCCR 2 cut(s) 3, 963
Eam1105I GACNNNNNGTC 1 cut(s) 1205
Ecl136II GAGCTC 1 cut(s) 551
Eco105I TACGTA 1 cut(s) 708
Eco130I CCWWGG 2 cut(s) 595, 947
Eco24I GRGCYC 2 cut(s) 125, 553
Eco31I GGTCTC 1 cut(s) 1638
Eco32I GATATC 2 cut(s) 409, 2029
Eco47I GGWCC 4 cut(s) 563, 914, 1424, 2102
Eco47III AGCGCT 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 551
Eco57I CTGAAG 3 cut(s) 1362, 1548, 2064
Eco88I CYCGRG 1 cut(s) 1350
EcoICRI GAGCTC 1 cut(s) 551
EcoRI GAATTC 2 cut(s) 1042, 1333
EcoRII CCWGG 7 cut(s) 350, 834, 1146, 1471, 1699, 1753, 2104
EcoRV GATATC 2 cut(s) 409, 2029
EcoT14I CCWWGG 2 cut(s) 595, 947
EcoT22I ATGCAT 1 cut(s) 871
EcoT38I GRGCYC 2 cut(s) 125, 553
ErhI CCWWGG 2 cut(s) 595, 947
FalI AAGNNNNNCTT 2 cut(s) 1163, 1195
FaqI GGGAC 1 cut(s) 368
FauI CCCGC 1 cut(s) 1117
FauNDI CATATG 1 cut(s) 1606
FblI GTMKAC 1 cut(s) 114
FokI GGATG 6 cut(s) 596, 747, 1143, 1163, 2129, 2177
FriOI GRGCYC 2 cut(s) 125, 553
FspBI CTAG 4 cut(s) 659, 731, 969, 1274
GlaI GCGC 5 cut(s) 82, 282, 670, 682, 1395
GsuI CTGGAG 5 cut(s) 314, 1242, 1455, 1494, 1620
HaeII RGCGCY 2 cut(s) 84, 284
HaeIII GGCC 5 cut(s) 5, 442, 594, 965, 1675
HapII CCGG 1 cut(s) 479
HgaI GACGC 1 cut(s) 2130
HhaI GCGC 5 cut(s) 83, 283, 671, 683, 1396
Hin1I GRCGYC 1 cut(s) 792
Hin6I GCGC 5 cut(s) 81, 281, 669, 681, 1394
HinP1I GCGC 5 cut(s) 81, 281, 669, 681, 1394
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HindIII AAGCTT 2 cut(s) 1770, 1932
HinfI GANTC 7 cut(s) 140, 446, 1694, 1877, 2060, 2068, 2120
HpaII CCGG 1 cut(s) 479
HphI GGTGA 6 cut(s) 152, 886, 1166, 1491, 1715, 2083
Hpy166II GTNNAC 2 cut(s) 115, 790
Hpy188I TCNGA 6 cut(s) 369, 451, 1114, 1870, 2116, 2158
Hpy8I GTNNAC 2 cut(s) 115, 790
Hpy99I CGWCG 2 cut(s) 987, 2146
HpyAV CCTTC 6 cut(s) 225, 289, 584, 631, 1420, 2065
HpyCH4III ACNGT 8 cut(s) 397, 573, 785, 911, 1024, 1523, 1553, 1994
HpyCH4IV ACGT 3 cut(s) 633, 707, 792
HpyF3I CTNAG 3 cut(s) 958, 1138, 1776
HpySE526I ACGT 3 cut(s) 633, 707, 792
Hsp92I GRCGYC 1 cut(s) 792
HspAI GCGC 5 cut(s) 81, 281, 669, 681, 1394
Kzo9I GATC 8 cut(s) 347, 540, 846, 979, 1077, 1300, 1888, 2001
LmnI GCTCC 7 cut(s) 481, 487, 548, 1223, 1474, 1513, 1800
LweI GCATC 4 cut(s) 725, 760, 878, 1336
MaeI CTAG 4 cut(s) 659, 731, 969, 1274
MaeII ACGT 3 cut(s) 633, 707, 792
MaeIII GTNAC 7 cut(s) 224, 931, 1133, 1477, 1513, 1684, 2089
MalI GATC 8 cut(s) 349, 542, 848, 981, 1079, 1302, 1890, 2003
MboI GATC 8 cut(s) 347, 540, 846, 979, 1077, 1300, 1888, 2001
MboII GAAGA 5 cut(s) 599, 851, 1355, 1541, 1793
MflI RGATCY 2 cut(s) 347, 2001
MhlI GDGCHC 3 cut(s) 125, 553, 1471
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 149, 1688, 1871, 2069
MmeI TCCRAC 2 cut(s) 2080, 2094
MnlI CCTC 9 cut(s) 142, 622, 671, 700, 806, 1415, 1530, 1579, 1808
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 871
MroXI GAANNNNTTC 2 cut(s) 1046, 1934
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 168, 459, 1055, 1164, 1241, 1364, 1962, 2212
MslI CAYNNNNRTG 2 cut(s) 387, 2021
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 203, 1601
MspI CCGG 1 cut(s) 479
MspR9I CCNGG 7 cut(s) 352, 836, 1148, 1473, 1701, 1755, 2106
Mva1269I GAATGC 1 cut(s) 1616
MvaI CCWGG 7 cut(s) 352, 836, 1148, 1473, 1701, 1755, 2106
NcoI CCATGG 2 cut(s) 595, 947
NdeI CATATG 1 cut(s) 1606
NdeII GATC 8 cut(s) 347, 540, 846, 979, 1077, 1300, 1888, 2001
NlaIV GGNNCC 6 cut(s) 199, 483, 945, 1018, 1567, 2040
NmuCI GTSAC 2 cut(s) 1133, 2089
NsiI ATGCAT 1 cut(s) 871
NspI RCATGY 5 cut(s) 531, 1544, 1589, 1857, 2135
OliI CACNNNNGTG 1 cut(s) 2021
PaeI GCATGC 1 cut(s) 1589
PaeR7I CTCGAG 1 cut(s) 1350
PagI TCATGA 1 cut(s) 1303
PaqCI CACCTGC 1 cut(s) 1901
PciI ACATGT 1 cut(s) 2131
PcsI WCGNNNNNNNCGW 1 cut(s) 789
PctI GAATGC 1 cut(s) 1616
PdmI GAANNNNTTC 2 cut(s) 1046, 1934
PfeI GAWTC 3 cut(s) 446, 2068, 2120
PflFI GACNNNGTC 1 cut(s) 998
PfoI TCCNGGA 1 cut(s) 834
PleI GAGTC 4 cut(s) 148, 1688, 1871, 2068
PpsI GAGTC 4 cut(s) 148, 1688, 1871, 2068
Ppu21I YACGTR 1 cut(s) 708
PscI ACATGT 1 cut(s) 2131
PsiI TTATAA 1 cut(s) 654
Psp124BI GAGCTC 1 cut(s) 553
Psp6I CCWGG 7 cut(s) 350, 834, 1146, 1471, 1699, 1753, 2104
PspGI CCWGG 7 cut(s) 350, 834, 1146, 1471, 1699, 1753, 2104
PspN4I GGNNCC 6 cut(s) 199, 483, 945, 1018, 1567, 2040
PspPI GGNCC 4 cut(s) 563, 914, 1424, 2102
PspXI VCTCGAGB 1 cut(s) 1350
PstI CTGCAG 3 cut(s) 722, 904, 1096
PsuI RGATCY 2 cut(s) 347, 2001
PsyI GACNNNGTC 1 cut(s) 998
PvuII CAGCTG 1 cut(s) 1601
RsaI GTAC 2 cut(s) 146, 1550
RsaNI GTAC 2 cut(s) 145, 1549
RseI CAYNNNNRTG 2 cut(s) 387, 2021
SacI GAGCTC 1 cut(s) 553
SaqAI TTAA 8 cut(s) 168, 459, 1055, 1164, 1241, 1364, 1962, 2212
Sau3AI GATC 8 cut(s) 347, 540, 846, 979, 1077, 1300, 1888, 2001
Sau96I GGNCC 4 cut(s) 563, 914, 1424, 2102
SchI GAGTC 4 cut(s) 149, 1688, 1871, 2069
ScrFI CCNGG 7 cut(s) 352, 836, 1148, 1473, 1701, 1755, 2106
SduI GDGCHC 3 cut(s) 125, 553, 1471
SfaNI GCATC 4 cut(s) 725, 760, 878, 1336
SfcI CTRYAG 6 cut(s) 393, 718, 741, 900, 1092, 1665
Sfr274I CTCGAG 1 cut(s) 1350
SinI GGWCC 4 cut(s) 563, 914, 1424, 2102
SlaI CTCGAG 1 cut(s) 1350
SmiMI CAYNNNNRTG 2 cut(s) 387, 2021
SmlI CTYRAG 2 cut(s) 1350, 2019
SmoI CTYRAG 2 cut(s) 1350, 2019
SnaBI TACGTA 1 cut(s) 708
SphI GCATGC 1 cut(s) 1589
SsiI CCGC 5 cut(s) 201, 966, 1124, 1583, 1589
SspI AATATT 3 cut(s) 1293, 1809, 2210
SspMI CTAG 4 cut(s) 659, 731, 969, 1274
SstI GAGCTC 1 cut(s) 553
StyD4I CCNGG 7 cut(s) 350, 834, 1146, 1471, 1699, 1753, 2104
StyI CCWWGG 2 cut(s) 595, 947
TaaI ACNGT 8 cut(s) 397, 573, 785, 911, 1024, 1523, 1553, 1994
TaiI ACGT 3 cut(s) 636, 710, 795
TaqI TCGA 5 cut(s) 385, 399, 985, 1351, 2058
TatI WGTACW 1 cut(s) 1548
TauI GCSGC 2 cut(s) 968, 1592
TfiI GAWTC 3 cut(s) 446, 2068, 2120
Tru1I TTAA 8 cut(s) 168, 459, 1055, 1164, 1241, 1364, 1962, 2212
Tru9I TTAA 8 cut(s) 168, 459, 1055, 1164, 1241, 1364, 1962, 2212
TscAI CASTG 8 cut(s) 268, 613, 727, 916, 966, 1044, 1345, 1999
TseFI GTSAC 2 cut(s) 1133, 2089
Tsp45I GTSAC 2 cut(s) 1133, 2089
TspGWI ACGGA 2 cut(s) 483, 576
TspRI CASTG 8 cut(s) 268, 613, 727, 916, 966, 1044, 1345, 1999
Tth111I GACNNNGTC 1 cut(s) 998
VpaK11BI GGWCC 4 cut(s) 563, 914, 1424, 2102
XapI RAATTY 3 cut(s) 1042, 1333, 1706
XceI RCATGY 5 cut(s) 531, 1544, 1589, 1857, 2135
XhoI CTCGAG 1 cut(s) 1350
XmiI GTMKAC 1 cut(s) 114
XmnI GAANNNNTTC 2 cut(s) 1046, 1934
XspI CTAG 4 cut(s) 659, 731, 969, 1274
ZraI GACGTC 1 cut(s) 793
Zsp2I ATGCAT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.