Rroxscaffold_4G00326670

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000257
Physical Location & Seq
Forward (+)
58820097 .. 58830635
10539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326670.1

Sequence Viewer

Length: 666 bp
ATGGAGTTGACATTATTAAAAATTTACAATAACAGTCCTGATGAGTTAATCTCATTCAATAAGGATCCTCCAGCAATTGATGCTTTTCATGCAACGGGGAAAGGGATACTAACCACAAACCCTTCAGGAAATGATGGCCAACTTGGTAACACAGAAAGTCCAACGCCATGGATGCTTACAGTTGCAGCAAGTAGCACAGACCGTCGGATCTTTGACAAGCTAGGTTGTTCTTGGAAAGGAAGCACGCTAGTTGGGATTCCATTGAACCCTTTCACATTAAATGGAACAAATTTTCCATTGGGATATGGAGACAGTCACTACTCTCAGTCCTCAGCCGGGAATTGTTCAGCTGGCTGCCTAGACACCGGCATACTAAAGAGTGAAGACATCAATAATCCTGCTGCACCTTTTGTCGCTTCATTCTCTTCATGTGGACCTAATCAAATTTTACCAGAAATTAGTAAGCCAGATATAACTGCCCCAGGGTTAGATATAACTGCTCTGAAATTCCCATGTTTTGAGTTATTAGAAGGGAAGGAGAATTTGAACACGTTCGTCCTTCTTTCAAGAGCAAGGGTGAGTCATAGCCTTCGCCAACGCCCGAATGCGAAGTTCTTGTCGAAAATAGTGTCCTTGGTTGCGAATTTGGTAGCCTATGCCGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

221

Amino Acids

23.56

Weight (kDa)

5.9

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 59, 72, 215
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 6 cut(s) 21, 289, 444, 506, 541, 643
AcuI CTGAAG 1 cut(s) 108
AfiI CCNNNNNNNGG 2 cut(s) 336, 660
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 4 cut(s) 58, 265, 547, 567
AjnI CCWGG 1 cut(s) 481
AloI GAACNNNNNNTCC 2 cut(s) 277, 309
AluBI AGCT 2 cut(s) 220, 350
AluI AGCT 2 cut(s) 220, 350
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 3 cut(s) 59, 72, 215
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 3 cut(s) 185, 354, 401
ApoI RAATTY 6 cut(s) 21, 289, 444, 506, 541, 643
ArsI GACNNNNNNTTYG 4 cut(s) 602, 614, 634, 646
Asp700I GAANNNNTTC 2 cut(s) 269, 551
AspS9I GGNCC 1 cut(s) 434
AsuC2I CCSGG 2 cut(s) 337, 661
AsuHPI GGTGA 1 cut(s) 589
AvaII GGWCC 1 cut(s) 434
BalI TGGCCA 1 cut(s) 138
BamHI GGATCC 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 390
BbvCI CCTCAGC 1 cut(s) 331
BbvI GCAGC 3 cut(s) 197, 341, 388
BccI CCATC 1 cut(s) 128
BciT130I CCWGG 1 cut(s) 483
BciVI GTATCC 1 cut(s) 99
BcnI CCSGG 2 cut(s) 337, 661
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 3 cut(s) 221, 248, 359
BfuI GTATCC 1 cut(s) 99
BisI GCNGC 3 cut(s) 186, 355, 402
BlsI GCNGC 3 cut(s) 187, 356, 403
Bme1390I CCNGG 3 cut(s) 337, 483, 661
Bme18I GGWCC 1 cut(s) 434
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 1 cut(s) 66
BmrFI CCNGG 3 cut(s) 337, 483, 661
BmsI GCATC 2 cut(s) 70, 162
BpiI GAAGAC 1 cut(s) 390
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 1 cut(s) 54
Bpu10I CCTNAGC 1 cut(s) 331
BpuMI CCSGG 2 cut(s) 337, 661
BsaJI CCNNGG 4 cut(s) 167, 481, 482, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 336, 660
Bse118I RCCGGY 1 cut(s) 365
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 4 cut(s) 167, 481, 482, 633
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 336, 660
BseMII CTCAG 2 cut(s) 338, 345
BseXI GCAGC 3 cut(s) 197, 341, 388
BsgI GTGCAG 1 cut(s) 387
BshFI GGCC 1 cut(s) 138
BsiSI CCGG 3 cut(s) 336, 366, 660
BslI CCNNNNNNNGG 2 cut(s) 336, 660
BsmAI GTCTC 1 cut(s) 303
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 1 cut(s) 138
Bsp143I GATC 2 cut(s) 64, 207
Bsp19I CCATGG 1 cut(s) 167
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 2 cut(s) 337, 344
BspLI GGNNCC 1 cut(s) 66
BspPI GGATC 3 cut(s) 59, 72, 215
BsrFI RCCGGY 1 cut(s) 365
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 4 cut(s) 167, 481, 482, 633
BssMI GATC 2 cut(s) 64, 207
BssT1I CCWWGG 2 cut(s) 167, 633
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 4 cut(s) 35, 181, 203, 314
Bst6I CTCTTC 1 cut(s) 430
BstAPI GCANNNNNTGC 1 cut(s) 80
BstC8I GCNNGC 2 cut(s) 245, 352
BstDEI CTNAG 2 cut(s) 324, 331
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 177
BstKTI GATC 2 cut(s) 67, 210
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 2 cut(s) 64, 207
BstMWI GCNNNNNNNGC 3 cut(s) 80, 89, 172
BstNI CCWGG 1 cut(s) 483
BstSCI CCNGG 3 cut(s) 335, 481, 659
BstV1I GCAGC 3 cut(s) 197, 341, 388
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 2 cut(s) 64, 207
BstXI CCANNNNNNTGG 1 cut(s) 168
BstYI RGATCY 2 cut(s) 64, 207
BsuI GTATCC 1 cut(s) 99
BsuRI GGCC 1 cut(s) 138
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 1 cut(s) 177
Cac8I GCNNGC 2 cut(s) 245, 352
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 434
CviAII CATG 4 cut(s) 89, 168, 429, 513
CviJI RGCY 8 cut(s) 138, 220, 335, 350, 354, 466, 588, 653
CviKI_1 RGCY 8 cut(s) 138, 220, 335, 350, 354, 466, 588, 653
DdeI CTNAG 2 cut(s) 324, 331
DpnI GATC 2 cut(s) 66, 209
DpnII GATC 2 cut(s) 64, 207
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 430
EarI CTCTTC 1 cut(s) 430
Eco130I CCWWGG 2 cut(s) 167, 633
Eco47I GGWCC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 2 cut(s) 167, 633
ErhI CCWWGG 2 cut(s) 167, 633
FaeI CATG 4 cut(s) 92, 171, 432, 516
FatI CATG 4 cut(s) 88, 167, 428, 512
Fnu4HI GCNGC 3 cut(s) 186, 355, 402
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 3 cut(s) 186, 355, 402
FspBI CTAG 3 cut(s) 221, 248, 359
GluI GCNGC 3 cut(s) 186, 355, 402
GsuI CTGGAG 1 cut(s) 54
HaeIII GGCC 1 cut(s) 138
HapII CCGG 3 cut(s) 336, 366, 660
Hin1II CATG 4 cut(s) 92, 171, 432, 516
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 2 cut(s) 256, 580
HpaII CCGG 3 cut(s) 336, 366, 660
HphI GGTGA 1 cut(s) 589
Hpy166II GTNNAC 2 cut(s) 9, 434
Hpy188I TCNGA 2 cut(s) 207, 504
Hpy188III TCNNGA 3 cut(s) 38, 126, 567
Hpy8I GTNNAC 2 cut(s) 9, 434
Hpy99I CGWCG 1 cut(s) 207
HpyAV CCTTC 5 cut(s) 132, 524, 529, 569, 599
HpyCH4III ACNGT 4 cut(s) 35, 181, 203, 314
HpyCH4IV ACGT 1 cut(s) 551
HpyCH4V TGCA 3 cut(s) 92, 185, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 89, 172
HpyF3I CTNAG 2 cut(s) 324, 331
HpySE526I ACGT 1 cut(s) 551
Hsp92II CATG 4 cut(s) 92, 171, 432, 516
Kzo9I GATC 2 cut(s) 64, 207
Lsp1109I GCAGC 3 cut(s) 197, 341, 388
LweI GCATC 2 cut(s) 70, 162
MaeI CTAG 3 cut(s) 221, 248, 359
MaeII ACGT 1 cut(s) 551
MaeIII GTNAC 2 cut(s) 146, 314
MalI GATC 2 cut(s) 66, 209
MboI GATC 2 cut(s) 64, 207
MboII GAAGA 2 cut(s) 395, 417
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 2 cut(s) 64, 207
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 9 cut(s) 21, 75, 289, 340, 444, 456, 506, 541, 643
MluNI TGGCCA 1 cut(s) 138
MlyI GAGTC 1 cut(s) 589
MmeI TCCRAC 2 cut(s) 185, 185
MnlI CCTC 2 cut(s) 78, 340
Mox20I TGGCCA 1 cut(s) 138
MroXI GAANNNNTTC 2 cut(s) 269, 551
MscI TGGCCA 1 cut(s) 138
MseI TTAA 3 cut(s) 17, 47, 278
Msp20I TGGCCA 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 3 cut(s) 336, 366, 660
MspR9I CCNGG 3 cut(s) 337, 483, 661
MunI CAATTG 1 cut(s) 75
Mva1269I GAATGC 1 cut(s) 610
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 3 cut(s) 80, 89, 172
NciI CCSGG 2 cut(s) 337, 661
NcoI CCATGG 1 cut(s) 167
NdeII GATC 2 cut(s) 64, 207
NlaIII CATG 4 cut(s) 92, 171, 432, 516
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 1 cut(s) 314
PasI CCCWGGG 1 cut(s) 482
PctI GAATGC 1 cut(s) 610
PdmI GAANNNNTTC 2 cut(s) 269, 551
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 3 cut(s) 187, 356, 403
PleI GAGTC 1 cut(s) 588
PpsI GAGTC 1 cut(s) 588
Psp6I CCWGG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 1 cut(s) 434
PsuI RGATCY 2 cut(s) 64, 207
PvuII CAGCTG 1 cut(s) 350
SaqAI TTAA 3 cut(s) 17, 47, 278
SatI GCNGC 3 cut(s) 186, 355, 402
Sau3AI GATC 2 cut(s) 64, 207
Sau96I GGNCC 1 cut(s) 434
SchI GAGTC 1 cut(s) 589
ScrFI CCNGG 3 cut(s) 337, 483, 661
SetI ASST 6 cut(s) 222, 226, 352, 409, 439, 554
SfaNI GCATC 2 cut(s) 70, 162
SinI GGWCC 1 cut(s) 434
Sse9I AATT 9 cut(s) 21, 75, 289, 340, 444, 456, 506, 541, 643
SspMI CTAG 3 cut(s) 221, 248, 359
StyD4I CCNGG 3 cut(s) 335, 481, 659
StyI CCWWGG 2 cut(s) 167, 633
TaaI ACNGT 4 cut(s) 35, 181, 203, 314
TaiI ACGT 1 cut(s) 554
TaqI TCGA 1 cut(s) 620
TasI AATT 9 cut(s) 21, 75, 289, 340, 444, 456, 506, 541, 643
TfiI GAWTC 1 cut(s) 256
Tru1I TTAA 3 cut(s) 17, 47, 278
Tru9I TTAA 3 cut(s) 17, 47, 278
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 3 cut(s) 185, 354, 401
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 3 cut(s) 77, 408, 417
VpaK11BI GGWCC 1 cut(s) 434
XapI RAATTY 6 cut(s) 21, 289, 444, 506, 541, 643
XmnI GAANNNNTTC 2 cut(s) 269, 551
XspI CTAG 3 cut(s) 221, 248, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.