Rmu_sc0008378.1_g000013

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008378.1
OG0000257
Physical Location & Seq
Reverse (-)
45660 .. 51771
6112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008378.1_g000013.1.cds

Sequence Viewer

Length: 2778 bp
atgacaacgtcagcgcctcctggagatcgaactagctcctccgccgccttctccacagccggcaaactctatcactgccaggtcatggccggggacaagtttattctgatcaacgatggattcagcggccttgatcttcctttggctctaaggcttgagaaggagatgttgaggacgacgaggagatataattatcattctacgccggaatcccgacaccagtccggtagccggattcccgattccggtcaccggtcaccggatttcggtcaccggtgggcggccggccactggtcaccggagtcgggcaaggtccatattgtgtacatggggtcacttccatataaggagtactctccattttctcatcaccatgatatgaaggaagtagtatctgtcttcccgagcacagcattacaacttcaaacaagaagatcttgggacttcatgggtttcactgagaaaattcatagacgaggaaatgttgagagtgatattattgtggatgcagtgattgactctggaatttggcctgaatcagaaagcttcaatgacgaagtttttggtcctcctcctaagaagtggaaaggtgcttgtatgggcggcttcaatttcacttgcaacaatttgagcttattgtgcaaagccaatgatgaagataggaaggttcacattgtatacttagggtcacttcctaatacggtgtactcaccatcgtctcaccaccttggtatattacagaaagttgtccagggaagaggttctctaaaaaattacctgatcagaagctacaaaaggagtttcaatggatttgctgccaatctgactgaccaggaaagagaaaaacttgttaacatgaaggaagtagtctccatcttccagagcacaaaattcaaactccaaacaacaagatcttgggattttatgggattccatgagtgtatcaaccgaaatgctactatcgaaagtgatgtaattattggtgttattgatagtggaatttggcctgaatcagagagctttcaagacaaaggctttggtctggctcctaaaaagtggaaaggtgcttgcaatggaggcaagaatttcacttgcaacaacaaactcattggagctcggtattactcatcactgcagtctgcaagagatgaagttggtcatggaagccacactgcttcaacagcagcagggaatgttgtgaagggtgttagcttttacggactagcacaaggtactgcaagaggaggagttccctctgcaagaattgcagcatatcaagtatgtgatttggattcgttctgtagcggacacgatgtgttggctgctttcgatgatgccattgctgatggagttgacatcattacactttccatcggaacagatcttcgatctgaattttacaaggatcctattgcaattggttcttttcatgccatggcaaaagggatactaacctccaactctgctggcaatgaaggtcctgcaggcgatactgtgtcaagtgcagcaccttggacacttacagttgcagcaagtagcattgatcgtcgaatcattgacaaggttgttcttggaaatggaagcatactagtcgggacttcagtgaacactttcacattaaatggatcaagttttcctctgatatatggaaaagatgcttcacgtcatgaatgctcggactcccaagctgggttatgtgatgaagattgccttgacagtgattcagttaagggaaagattgtgctatgtgatgcattcgctgaagcagattttgtggctcataaagctggtgcactaggttctattttaaataatgacggaactggtgatttttctaatgttgtctcattacctgcaatagctttaagctatgaaaactataatgcagtcaagttttacatgaactccactagagatcctcgagcaaacatattaagaagtgaagtcataaatgacccggctgcaccaattgtcgcttccttctcttcacgtggacccaatccaattacacctgacattataaagccagatataactgcgcctggtgtagatattttggctgcatattcgactgttgcctcaatttcagagagccccgaagacaagaggcatgtaaagtaccatatattatctggaacctccatgtcttgccctcatgctgctgctgttgctgcgtatgttaaatcattccatcccaattggtctccatcagctataaaatcatctcttatgactactgcttggccaatgaataataccaacaatgcacctgctgagtttgcttatggatctggacatgtcaatcctgtaaaagctatagatcccgggcttgtttatgaaacttctaaagaagatttcataaacctactttgcatggactatgatgaagccaaagtgagactgatatcaggagataacagcacttgcccaacaggctctgataaaggatcaccaaaggatcttaactacccttcaatggctgcagcaaacgttacaccgaagaaaccttttatgattaactttcatagaacagttaaaaatgttggccttgcaaacactacttacaatgccaccatcttccaaagttccaaagacgtggatatcaaagttgtgcctgaatttctatccttcaagtccttgaatgaggagaagagctttgatgtggctgttgttggaaaaggtttgataggtggatcactaatttcttcgtcactggtttggtctgatggtgttcataacgttaggagtccagttgtggtgcgtactgaaatagcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

925

Amino Acids

100.08

Weight (kDa)

6.48

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2021
AarI CACCTGC 1 cut(s) 2278
Acc36I ACCTGC 2 cut(s) 1861, 2278
AccB7I CCANNNNNTGG 1 cut(s) 85
AccI GTMKAC 1 cut(s) 678
AciI CCGC 6 cut(s) 42, 45, 126, 281, 603, 1305
AclI AACGTT 2 cut(s) 2490, 2739
AclWI GGATC 9 cut(s) 1400, 1413, 1633, 1910, 2296, 2315, 2455, 2466, 2701
AcoI YGGCCR 4 cut(s) 87, 282, 286, 2243
AcsI RAATTY 7 cut(s) 465, 525, 890, 999, 1084, 1394, 2618
AcuI CTGAAG 2 cut(s) 1584, 1782
AcvI CACGTG 1 cut(s) 1991
AdeI CACNNNGTG 1 cut(s) 1315
AfaI GTAC 6 cut(s) 326, 353, 707, 1234, 2120, 2764
AflIII ACRYGT 1 cut(s) 2296
AgeI ACCGGT 2 cut(s) 252, 273
AhlI ACTAGT 1 cut(s) 1588
AjiI CACGTC 2 cut(s) 1664, 2596
AjnI CCWGG 5 cut(s) 19, 78, 750, 831, 2041
AjuI GAANNNNNNNTTGG 2 cut(s) 1960, 1992
Alw21I GWGCWC 4 cut(s) 410, 887, 1117, 1795
Alw26I GTCTC 5 cut(s) 723, 874, 1849, 2208, 2392
Alw44I GTGCAC 1 cut(s) 1791
AlwI GGATC 9 cut(s) 1400, 1413, 1633, 1910, 2296, 2315, 2455, 2466, 2701
AlwNI CAGNNNCTG 1 cut(s) 2438
Ama87I CYCGRG 3 cut(s) 403, 1920, 2324
AoxI GGCC 8 cut(s) 87, 127, 282, 286, 530, 1004, 2243, 2545
ApaLI GTGCAC 1 cut(s) 1791
ApoI RAATTY 7 cut(s) 465, 525, 890, 999, 1084, 1394, 2618
ArsI GACNNNNNNTTYG 4 cut(s) 545, 577, 1019, 1051
AsiGI ACCGGT 2 cut(s) 252, 273
Asp700I GAANNNNTTC 2 cut(s) 760, 1610
AspLEI GCGC 2 cut(s) 16, 2041
AspS9I GGNCC 4 cut(s) 313, 566, 1478, 1994
AsuC2I CCSGG 4 cut(s) 91, 1958, 2325, 2326
AsuHPI GGTGA 9 cut(s) 242, 249, 263, 288, 362, 702, 713, 1838, 2442
AvaI CYCGRG 3 cut(s) 403, 1920, 2324
AvaII GGWCC 4 cut(s) 313, 566, 1478, 1994
BaeGI GKGCMC 1 cut(s) 1795
BalI TGGCCA 1 cut(s) 2245
BamHI GGATCC 1 cut(s) 1405
BanII GRGCYC 2 cut(s) 1117, 2096
BbrPI CACGTG 1 cut(s) 1991
BbsI GAAGAC 2 cut(s) 391, 2106
Bbv12I GWGCWC 4 cut(s) 410, 887, 1117, 1795
BccI CCATC 9 cut(s) 110, 721, 881, 1340, 1379, 2199, 2215, 2582, 2720
BcgI CGANNNNNNTGC 2 cut(s) 1573, 1607
BciT130I CCWGG 5 cut(s) 21, 80, 752, 833, 2043
BciVI GTATCC 1 cut(s) 1440
BclI TGATCA 2 cut(s) 108, 780
BcnI CCSGG 4 cut(s) 91, 1958, 2325, 2326
BcoDI GTCTC 5 cut(s) 723, 874, 1849, 2208, 2392
BcuI ACTAGT 1 cut(s) 1588
BfaI CTAG 5 cut(s) 33, 1223, 1589, 1796, 1911
BfmI CTRYAG 5 cut(s) 1133, 1300, 1482, 2316, 2481
BfoI RGCGCY 1 cut(s) 17
BfuAI ACCTGC 2 cut(s) 1861, 2278
BfuI GTATCC 1 cut(s) 1440
BglI GCCNNNNNGGC 1 cut(s) 2433
BglII AGATCT 3 cut(s) 434, 911, 1381
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 9 cut(s) 21, 80, 91, 752, 833, 1958, 2043, 2325, 2326
Bme18I GGWCC 4 cut(s) 313, 566, 1478, 1994
BmeT110I CYCGRG 3 cut(s) 403, 1920, 2324
BmgBI CACGTC 2 cut(s) 1664, 2596
BmgT120I GGNCC 4 cut(s) 313, 566, 1478, 1994
BmiI GGNNCC 4 cut(s) 1047, 1407, 1996, 2137
BmrFI CCNGG 9 cut(s) 21, 80, 91, 752, 833, 1958, 2043, 2325, 2326
BmsI GCATC 4 cut(s) 496, 1324, 1645, 1741
BoxI GACNNNNGTC 1 cut(s) 220
BpiI GAAGAC 2 cut(s) 391, 2106
BpmI CTGGAG 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 4 cut(s) 91, 1958, 2325, 2326
BsaAI YACGTR 1 cut(s) 1991
BsaI GGTCTC 1 cut(s) 2208
BsaJI CCNNGG 6 cut(s) 90, 727, 751, 1434, 1511, 2324
BsaWI WCCGGW 6 cut(s) 224, 245, 252, 259, 273, 298
BsaXI ACNNNNNCTCC 2 cut(s) 1889, 1919
Bse118I RCCGGY 4 cut(s) 59, 252, 273, 284
Bse1I ACTGG 5 cut(s) 220, 296, 1828, 2718, 2750
Bse3DI GCAATG 3 cut(s) 1078, 1338, 1477
BseBI CCWGG 5 cut(s) 21, 80, 752, 833, 2043
BseDI CCNNGG 6 cut(s) 90, 727, 751, 1434, 1511, 2324
BseGI GGATG 2 cut(s) 511, 2191
BseMI GCAATG 3 cut(s) 1078, 1338, 1477
BseMII CTCAG 2 cut(s) 450, 2265
BseNI ACTGG 5 cut(s) 220, 296, 1828, 2718, 2750
BseRI GAGGAG 6 cut(s) 28, 196, 561, 1257, 1260, 2660
BseSI GKGCMC 1 cut(s) 1795
BseX3I CGGCCG 1 cut(s) 282
BseYI CCCAGC 1 cut(s) 1688
BsgI GTGCAG 2 cut(s) 1524, 1947
Bsh1285I CGRYCG 1 cut(s) 285
BshFI GGCC 8 cut(s) 89, 129, 284, 288, 532, 1006, 2245, 2547
BshTI ACCGGT 2 cut(s) 252, 273
BsiEI CGRYCG 1 cut(s) 285
BsiHKAI GWGCWC 4 cut(s) 410, 887, 1117, 1795
BsiHKCI CYCGRG 3 cut(s) 403, 1920, 2324
BslFI GGGAC 3 cut(s) 107, 455, 1609
BsmAI GTCTC 5 cut(s) 723, 874, 1849, 2208, 2392
BsmBI CGTCTC 1 cut(s) 723
BsmFI GGGAC 3 cut(s) 107, 455, 1609
BsmI GAATGC 2 cut(s) 1676, 1754
BsnI GGCC 8 cut(s) 89, 129, 284, 288, 532, 1006, 2245, 2547
Bso31I GGTCTC 1 cut(s) 2208
BsoBI CYCGRG 3 cut(s) 403, 1920, 2324
Bsp1286I GDGCHC 5 cut(s) 410, 887, 1117, 1795, 2096
Bsp1407I TGTACA 1 cut(s) 324
Bsp19I CCATGG 1 cut(s) 1434
BspACI CCGC 6 cut(s) 42, 45, 126, 281, 603, 1305
BspANI GGCC 8 cut(s) 89, 129, 284, 288, 532, 1006, 2245, 2547
BspCNI CTCAG 2 cut(s) 451, 2266
BspHI TCATGA 1 cut(s) 1666
BspLI GGNNCC 4 cut(s) 1047, 1407, 1996, 2137
BspMAI CTGCAG 3 cut(s) 1137, 1486, 2485
BspMI ACCTGC 2 cut(s) 1861, 2278
BspPI GGATC 9 cut(s) 1400, 1413, 1633, 1910, 2296, 2315, 2455, 2466, 2701
BspQI GCTCTTC 1 cut(s) 2645
BspTNI GGTCTC 1 cut(s) 2208
BsrDI GCAATG 3 cut(s) 1078, 1338, 1477
BsrFI RCCGGY 4 cut(s) 59, 252, 273, 284
BsrGI TGTACA 1 cut(s) 324
BsrI ACTGG 5 cut(s) 220, 296, 1828, 2718, 2750
BssAI RCCGGY 4 cut(s) 59, 252, 273, 284
BssECI CCNNGG 6 cut(s) 90, 727, 751, 1434, 1511, 2324
BssNAI GTATAC 1 cut(s) 679
BssT1I CCWWGG 3 cut(s) 727, 1434, 1511
Bst1107I GTATAC 1 cut(s) 679
Bst2UI CCWGG 5 cut(s) 21, 80, 752, 833, 2043
Bst4CI ACNGT 6 cut(s) 703, 1495, 1525, 1718, 2074, 2533
Bst6I CTCTTC 3 cut(s) 751, 1990, 2645
BstAPI GCANNNNNTGC 1 cut(s) 1265
BstAUI TGTACA 1 cut(s) 324
BstBAI YACGTR 1 cut(s) 1991
BstC8I GCNNGC 5 cut(s) 61, 286, 1069, 1468, 1486
BstDEI CTNAG 5 cut(s) 149, 459, 576, 682, 2274
BstDSI CCRYGG 1 cut(s) 1434
BstEII GGTNACC 4 cut(s) 248, 255, 269, 294
BstF5I GGATG 2 cut(s) 511, 2191
BstH2I RGCGCY 1 cut(s) 17
BstHHI GCGC 2 cut(s) 16, 2041
BstMAI GTCTC 5 cut(s) 723, 874, 1849, 2208, 2392
BstMCI CGRYCG 1 cut(s) 285
BstMWI GCNNNNNNNGC 9 cut(s) 639, 1077, 1181, 1265, 1784, 2168, 2171, 2279, 2433
BstNI CCWGG 5 cut(s) 21, 80, 752, 833, 2043
BstNSI RCATGY 2 cut(s) 2114, 2300
BstPAI GACNNNNGTC 1 cut(s) 220
BstPI GGTNACC 4 cut(s) 248, 255, 269, 294
BstSCI CCNGG 9 cut(s) 19, 78, 89, 750, 831, 1956, 2041, 2323, 2324
BstSFI CTRYAG 5 cut(s) 1133, 1300, 1482, 2316, 2481
BstSLI GKGCMC 1 cut(s) 1795
BstV2I GAAGAC 2 cut(s) 391, 2106
BstX2I RGATCY 8 cut(s) 434, 911, 1381, 1405, 1915, 2288, 2320, 2458
BstXI CCANNNNNNTGG 1 cut(s) 2596
BstYI RGATCY 8 cut(s) 434, 911, 1381, 1405, 1915, 2288, 2320, 2458
BstZ17I GTATAC 1 cut(s) 679
BstZI CGGCCG 1 cut(s) 282
BsuI GTATCC 1 cut(s) 1440
BsuRI GGCC 8 cut(s) 89, 129, 284, 288, 532, 1006, 2245, 2547
BtgI CCRYGG 1 cut(s) 1434
BtrI CACGTC 2 cut(s) 1664, 2596
BtsCI GGATG 2 cut(s) 511, 2191
BtsI GCAGTG 4 cut(s) 73, 516, 1130, 1170
BtsIMutI CAGTG 9 cut(s) 73, 289, 456, 516, 1130, 1170, 1608, 1723, 2711
BveI ACCTGC 2 cut(s) 1861, 2278
Cac8I GCNNGC 5 cut(s) 61, 286, 1069, 1468, 1486
CaiI CAGNNNCTG 1 cut(s) 2438
CciI TCATGA 1 cut(s) 1666
CfoI GCGC 2 cut(s) 16, 2041
Cfr10I RCCGGY 4 cut(s) 59, 252, 273, 284
Cfr13I GGNCC 4 cut(s) 313, 566, 1478, 1994
Cfr9I CCCGGG 1 cut(s) 2324
Csp6I GTAC 6 cut(s) 325, 352, 706, 1233, 2119, 2763
CspAI ACCGGT 2 cut(s) 252, 273
CviQI GTAC 6 cut(s) 325, 352, 706, 1233, 2119, 2763
DdeI CTNAG 5 cut(s) 149, 459, 576, 682, 2274
DraI TTTAAA 1 cut(s) 1809
DraIII CACNNNGTG 1 cut(s) 1315
EaeI YGGCCR 4 cut(s) 87, 282, 286, 2243
EagI CGGCCG 1 cut(s) 282
Eam1104I CTCTTC 3 cut(s) 751, 1990, 2645
EarI CTCTTC 3 cut(s) 751, 1990, 2645
EciI GGCGGA 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 1115
EclXI CGGCCG 1 cut(s) 282
Eco130I CCWWGG 3 cut(s) 727, 1434, 1511
Eco24I GRGCYC 2 cut(s) 1117, 2096
Eco31I GGTCTC 1 cut(s) 2208
Eco32I GATATC 2 cut(s) 2406, 2602
Eco47I GGWCC 4 cut(s) 313, 566, 1478, 1994
Eco52I CGGCCG 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 1115
Eco57I CTGAAG 2 cut(s) 1584, 1782
Eco72I CACGTG 1 cut(s) 1991
Eco88I CYCGRG 3 cut(s) 403, 1920, 2324
Eco91I GGTNACC 4 cut(s) 248, 255, 269, 294
EcoICRI GAGCTC 1 cut(s) 1115
EcoO109I RGGNCCY 1 cut(s) 1478
EcoO65I GGTNACC 4 cut(s) 248, 255, 269, 294
EcoRII CCWGG 5 cut(s) 19, 78, 750, 831, 2041
EcoRV GATATC 2 cut(s) 2406, 2602
EcoT14I CCWWGG 3 cut(s) 727, 1434, 1511
EcoT22I ATGCAT 1 cut(s) 1756
EcoT38I GRGCYC 2 cut(s) 1117, 2096
ErhI CCWWGG 3 cut(s) 727, 1434, 1511
Esp3I CGTCTC 1 cut(s) 723
FalI AAGNNNNNCTT 4 cut(s) 421, 453, 1695, 1727
FaqI GGGAC 3 cut(s) 107, 455, 1609
FbaI TGATCA 2 cut(s) 108, 780
FblI GTMKAC 1 cut(s) 678
FokI GGATG 2 cut(s) 518, 2178
FriOI GRGCYC 2 cut(s) 1117, 2096
FseI GGCCGGCC 1 cut(s) 288
FspBI CTAG 5 cut(s) 33, 1223, 1589, 1796, 1911
GlaI GCGC 2 cut(s) 15, 2040
GsaI CCCAGC 1 cut(s) 1692
GsuI CTGGAG 1 cut(s) 42
HaeII RGCGCY 1 cut(s) 17
HaeIII GGCC 8 cut(s) 89, 129, 284, 288, 532, 1006, 2245, 2547
HhaI GCGC 2 cut(s) 16, 2041
Hin6I GCGC 2 cut(s) 14, 2039
HinP1I GCGC 2 cut(s) 14, 2039
HincII GTYRAC 2 cut(s) 853, 1354
HindII GTYRAC 2 cut(s) 853, 1354
HindIII AAGCTT 1 cut(s) 544
HpaI GTTAAC 1 cut(s) 853
HphI GGTGA 9 cut(s) 242, 249, 263, 288, 362, 702, 713, 1838, 2442
Hpy166II GTNNAC 9 cut(s) 325, 670, 679, 706, 853, 1354, 1606, 1793, 1994
Hpy8I GTNNAC 9 cut(s) 325, 670, 679, 706, 853, 1354, 1606, 1793, 1994
Hpy99I CGWCG 2 cut(s) 181, 1551
HpyCH4III ACNGT 6 cut(s) 703, 1495, 1525, 1718, 2074, 2533
HpyCH4IV ACGT 6 cut(s) 8, 1663, 1990, 2490, 2595, 2739
HpyF10VI GCNNNNNNNGC 9 cut(s) 639, 1077, 1181, 1265, 1784, 2168, 2171, 2279, 2433
HpyF3I CTNAG 5 cut(s) 149, 459, 576, 682, 2274
HpySE526I ACGT 6 cut(s) 8, 1663, 1990, 2490, 2595, 2739
HspAI GCGC 2 cut(s) 14, 2039
KroI GCCGGC 2 cut(s) 59, 284
KroNI GCCGGC 2 cut(s) 61, 286
Ksp22I TGATCA 2 cut(s) 108, 780
KspAI GTTAAC 1 cut(s) 853
LguI GCTCTTC 1 cut(s) 2645
LmnI GCTCC 3 cut(s) 41, 1051, 1112
LweI GCATC 4 cut(s) 496, 1324, 1645, 1741
MaeI CTAG 5 cut(s) 33, 1223, 1589, 1796, 1911
MaeII ACGT 6 cut(s) 8, 1663, 1990, 2490, 2595, 2739
MaeIII GTNAC 8 cut(s) 248, 255, 269, 294, 333, 687, 2491, 2709
MfeI CAATTG 3 cut(s) 1416, 1968, 2197
MflI RGATCY 8 cut(s) 434, 911, 1381, 1405, 1915, 2288, 2320, 2458
MhlI GDGCHC 5 cut(s) 410, 887, 1117, 1795, 2096
MlsI TGGCCA 1 cut(s) 2245
MluNI TGGCCA 1 cut(s) 2245
MlyI GAGTC 4 cut(s) 311, 512, 1673, 2755
MmeI TCCRAC 2 cut(s) 1482, 2653
Mox20I TGGCCA 1 cut(s) 2245
Mph1103I ATGCAT 1 cut(s) 1756
MroNI GCCGGC 2 cut(s) 59, 284
MroXI GAANNNNTTC 2 cut(s) 760, 1610
MscI TGGCCA 1 cut(s) 2245
MslI CAYNNNNRTG 1 cut(s) 372
Msp20I TGGCCA 1 cut(s) 2245
MspA1I CMGCKG 1 cut(s) 126
MspR9I CCNGG 9 cut(s) 21, 80, 91, 752, 833, 1958, 2043, 2325, 2326
MunI CAATTG 3 cut(s) 1416, 1968, 2197
Mva1269I GAATGC 2 cut(s) 1676, 1754
MvaI CCWGG 5 cut(s) 21, 80, 752, 833, 2043
MwoI GCNNNNNNNGC 9 cut(s) 639, 1077, 1181, 1265, 1784, 2168, 2171, 2279, 2433
NaeI GCCGGC 2 cut(s) 61, 286
NciI CCSGG 4 cut(s) 91, 1958, 2325, 2326
NcoI CCATGG 1 cut(s) 1434
NgoMIV GCCGGC 2 cut(s) 59, 284
NlaIV GGNNCC 4 cut(s) 1047, 1407, 1996, 2137
NmuCI GTSAC 7 cut(s) 248, 255, 269, 294, 333, 687, 2709
NsiI ATGCAT 1 cut(s) 1756
NspI RCATGY 2 cut(s) 2114, 2300
PaeR7I CTCGAG 1 cut(s) 1920
PagI TCATGA 1 cut(s) 1666
PaqCI CACCTGC 1 cut(s) 2278
PciI ACATGT 1 cut(s) 2296
PciSI GCTCTTC 1 cut(s) 2645
PctI GAATGC 2 cut(s) 1676, 1754
PdiI GCCGGC 2 cut(s) 61, 286
PdmI GAANNNNTTC 2 cut(s) 760, 1610
PflFI GACNNNGTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 19
PinAI ACCGGT 2 cut(s) 252, 273
PleI GAGTC 4 cut(s) 310, 512, 1673, 2754
PmaCI CACGTG 1 cut(s) 1991
PmlI CACGTG 1 cut(s) 1991
PpsI GAGTC 4 cut(s) 310, 512, 1673, 2754
Ppu21I YACGTR 1 cut(s) 1991
PpuMI RGGWCCY 1 cut(s) 1478
PscI ACATGT 1 cut(s) 2296
PshAI GACNNNNGTC 1 cut(s) 220
PsiI TTATAA 1 cut(s) 2021
Psp124BI GAGCTC 1 cut(s) 1117
Psp1406I AACGTT 2 cut(s) 2490, 2739
Psp5II RGGWCCY 1 cut(s) 1478
Psp6I CCWGG 5 cut(s) 19, 78, 750, 831, 2041
PspCI CACGTG 1 cut(s) 1991
PspEI GGTNACC 4 cut(s) 248, 255, 269, 294
PspFI CCCAGC 1 cut(s) 1688
PspGI CCWGG 5 cut(s) 19, 78, 750, 831, 2041
PspN4I GGNNCC 4 cut(s) 1047, 1407, 1996, 2137
PspPI GGNCC 4 cut(s) 313, 566, 1478, 1994
PspPPI RGGWCCY 1 cut(s) 1478
PspXI VCTCGAGB 1 cut(s) 1920
PstI CTGCAG 3 cut(s) 1137, 1486, 2485
PstNI CAGNNNCTG 1 cut(s) 2438
PsuI RGATCY 8 cut(s) 434, 911, 1381, 1405, 1915, 2288, 2320, 2458
PsyI GACNNNGTC 1 cut(s) 7
RigI GGCCGGCC 1 cut(s) 288
RsaI GTAC 6 cut(s) 326, 353, 707, 1234, 2120, 2764
RsaNI GTAC 6 cut(s) 325, 352, 706, 1233, 2119, 2763
RseI CAYNNNNRTG 1 cut(s) 372
SacI GAGCTC 1 cut(s) 1117
SapI GCTCTTC 1 cut(s) 2645
Sau96I GGNCC 4 cut(s) 313, 566, 1478, 1994
SbfI CCTGCAGG 1 cut(s) 1486
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 4 cut(s) 311, 512, 1673, 2755
ScrFI CCNGG 9 cut(s) 21, 80, 91, 752, 833, 1958, 2043, 2325, 2326
SdaI CCTGCAGG 1 cut(s) 1486
SduI GDGCHC 5 cut(s) 410, 887, 1117, 1795, 2096
SfaNI GCATC 4 cut(s) 496, 1324, 1645, 1741
SfcI CTRYAG 5 cut(s) 1133, 1300, 1482, 2316, 2481
Sfr274I CTCGAG 1 cut(s) 1920
SgrAI CRCCGGYG 1 cut(s) 273
SinI GGWCC 4 cut(s) 313, 566, 1478, 1994
SlaI CTCGAG 1 cut(s) 1920
SmaI CCCGGG 1 cut(s) 2326
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 155, 1920
SmoI CTYRAG 2 cut(s) 155, 1920
SpeI ACTAGT 1 cut(s) 1588
Sse8387I CCTGCAGG 1 cut(s) 1486
SsiI CCGC 6 cut(s) 42, 45, 126, 281, 603, 1305
SspMI CTAG 5 cut(s) 33, 1223, 1589, 1796, 1911
SstI GAGCTC 1 cut(s) 1117
StyD4I CCNGG 9 cut(s) 19, 78, 89, 750, 831, 1956, 2041, 2323, 2324
StyI CCWWGG 3 cut(s) 727, 1434, 1511
TaaI ACNGT 6 cut(s) 703, 1495, 1525, 1718, 2074, 2533
TaiI ACGT 6 cut(s) 11, 1666, 1993, 2493, 2598, 2742
TaqI TCGA 7 cut(s) 28, 963, 1329, 1387, 1549, 1921, 2069
TaqII GACCGA 1 cut(s) 257
TatI WGTACW 3 cut(s) 324, 351, 705
TauI GCSGC 4 cut(s) 47, 129, 284, 606
TscAI CASTG 9 cut(s) 80, 296, 463, 516, 1137, 1177, 1608, 1723, 2718
TseFI GTSAC 7 cut(s) 248, 255, 269, 294, 333, 687, 2709
Tsp45I GTSAC 7 cut(s) 248, 255, 269, 294, 333, 687, 2709
TspGWI ACGGA 2 cut(s) 1233, 1833
TspMI CCCGGG 1 cut(s) 2324
TspRI CASTG 9 cut(s) 80, 296, 463, 516, 1137, 1177, 1608, 1723, 2718
Tth111I GACNNNGTC 1 cut(s) 7
Van91I CCANNNNNTGG 1 cut(s) 85
VneI GTGCAC 1 cut(s) 1791
VpaK11BI GGWCC 4 cut(s) 313, 566, 1478, 1994
XapI RAATTY 7 cut(s) 465, 525, 890, 999, 1084, 1394, 2618
XceI RCATGY 2 cut(s) 2114, 2300
XcmI CCANNNNNNNNNTGG 1 cut(s) 2129
XhoI CTCGAG 1 cut(s) 1920
XmaI CCCGGG 1 cut(s) 2324
XmiI GTMKAC 1 cut(s) 678
XmnI GAANNNNTTC 2 cut(s) 760, 1610
XspI CTAG 5 cut(s) 33, 1223, 1589, 1796, 1911
ZrmI AGTACT 1 cut(s) 353
Zsp2I ATGCAT 1 cut(s) 1756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.