Rroxscaffold_1G00014230

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000257
Physical Location & Seq
Reverse (-)
17532443 .. 17543844
11402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00014230.1

Sequence Viewer

Length: 2301 bp
ATGGAGCCGATACTTGGAGACGACACTCGGGGCTTAGAGACGACGAAGGCGAGGCGAGCCGAGGAGGAGCGCGAGGCGGATAATGTGGCTTCGGACCGAATGACGGCCATCCATGGAGCTCTTCTGTTTTATCACACCTTGTCAATTCTCATCCTCACTCTGAGTTTGTTATGCAGAGCCACTTGCGACGATAGAAAGGTCTATATTGTGTACTTGGGGTCACTTTCTGATGGGTTGTACTCGCCGTCGTCTCACCATCTTAGTATTCTACAGAGAGTTGTCCAGGACAGTTCTCCTGAAAATGTTTTGGTAAGAAGTTACAAAAGGAGTTTCAATGGATTTGCTGCCAGACTCACTGACCAGGAAAGAAAAAAACTTTCTAAAATGAAGGAAGTAGTCTCTGTTTTTCCAAGCAGAACGTTGCAACTTCAAACAACAAGATCTTGGGACTTCATGGGATTCAATGAGAAAATTGGCCGCAATGCCACAGTTGAGAGTGATACTATTGTGGGTGTCATTGACAGTGGAATTTGGCCTGAATCTGAGAGTTTTAAGGAGGAAGGTTTTGGTCCTCCACCCAAGAAGTGGAAAGGTGCTTGTGAAGGGGGCAACAAATTTACTTGCAACAACAAGCTTATTGGAGCTCGGTATTACTCGTCAACAGAGTCTGCAAGGGATGACACAGGTCACGGAACCCACACTGCCTCAACGGCAGCAGGAAATGCTGTAAGAGATGTAAGCTTTTATGGACTGGCACAAGTTACTGCAAGAGGAGGAGTTCCCTCTGCAAGAATTGCCGCATACAAAGTCTGTACCCCAGGCACATTGTGTGGTGCACATGATACCCTGGCTGCTTTTGACGATGCAATTGCTGATGGAGTCGATATTATTACAGCTTCAGTTGCATTTAGTATGATACAAGAATTCGATGTGGATCCTCTTGCAATTGGTACTTTTCACGCAATGGAGAAAGGGATACTAACCTCACATGCTGCGGGCAACAATGGACCTTCCGGTGCTACTGTATCAAGTGTAGCACCTTGGGTGCTTACAGTTGCAGCAAGTAGCATGGACCGTCGGATCATTGACAAGGTCATCCTTGGAAATGGAAGGACAGTAATTGGGAATTCTGTGAACTCCTTCACACTAAATGGGACAAGTTTTCCTCTGATACATGGAAAAAATGCTTCAAAGAATTGCACAGAAACAAGTGCGGGAGGTTGTGAACAAGGTTGCCTGGACAGTAGTCTAGTGAAGGGAAAGGTTGTGCTATGTGATAAATATGGACTTCTTGAAGCATTTCAATCTGGTGCACTGGGTTCAATTTTACCCAATTTTCGAGCTGTTGATGATGCCTCTTATGTTCTCCCATTAGCTGGCACGACTATAAACAACGAAAACTATAATGTAGTCATGTCATACTTGAACTCCACCAGAGATCCTCGCGTGAACATAATAAAAAGTGAAGTCATAAAAAATCCTGCTGTCCCTGCTGTTGCTTCATTCTCTGCACGTGGACCAAATTTAATTTTACCTGATATTATCAAGCCAGATATAAGTGCCCCAGGGATAGAAATTCTGGCTGCATTTTCGTCTGCTCCTATCTCAACCACTCCTGGAGATATGAGGCGTGTTAAGTACAATATACAATCTGGAACCTCCATGGCTTGCCCCCATGCTGCTGGTGTAGCTACCTATGTTAAAACCTTCCACCCTGATTGGTCTCCTGCGGCCATGAAATCATCTATTATGACTACTGCTTGGCCCATGAATGATCCCAGCAGCAATGTTTCCACTGGTGAATTCGCTTATGGATCAGGCCATATCAATCCATTAAAAGCTATTGACCCCGGGCTTGTATATGATGCTTCCAAAGAAGATTACATAAAGTTGCTGTGCATCAGCTATGATGAAAGCAGAGTTAGACTTGTATCAGGAGATAACAGCACATGCCCTACAGGTCCTGACAAGGGATCTCCGAAGGATCATAATTACCCTTCGATGGGAGCCAAAGTTACCGCAATGCAACCATTTACAGTTACATTTAATAGAAGATTTAAAAATGTTGGCAATGCAAACTCCACTTATGTAGCCAAAATCTCCCCCAATTCCAAACTCGATATTAAAGTGTTGCCGGAAGTCCTATCCTTCAAGTCCTTAAATGAGGAGAAGACTTTTAATGTGACTGTTTCTGGAAGCTATTTACCTTTTGAATCACGAGCATCTGCTTCACTGGTTTGGTCTGATGGTACTCATGGCGTTAGAAGTCCGATTGTTGTGTACACCGTGCCTAGAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

766

Amino Acids

82.2

Weight (kDa)

7.54

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 67 - 144 3e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 166 - 605 6.5e-41 Subtilase family
fn3_6 PF17766 662 - 760 5e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 585, 1376
AccII CGCG 2 cut(s) 72, 1446
AciI CCGC 7 cut(s) 77, 478, 798, 995, 1214, 1730, 2019
AclI AACGTT 1 cut(s) 419
AclWI GGATC 8 cut(s) 929, 942, 1088, 1433, 1769, 1822, 1981, 1992
AcoI YGGCCR 3 cut(s) 105, 475, 1731
AcsI RAATTY 7 cut(s) 528, 614, 923, 1126, 1522, 1575, 1802
AcuI CTGAAG 1 cut(s) 882
AcvI CACGTG 1 cut(s) 1514
AdeI CACNNNGTG 1 cut(s) 828
AfaI GTAC 7 cut(s) 212, 239, 814, 952, 1640, 2251, 2282
AfiI CCNNNNNNNGG 7 cut(s) 14, 103, 585, 1376, 1970, 2002, 2003
AjnI CCWGG 7 cut(s) 282, 360, 817, 846, 1236, 1564, 1615
Alw21I GWGCWC 4 cut(s) 121, 646, 838, 1315
Alw26I GTCTC 5 cut(s) 12, 32, 255, 403, 1728
Alw44I GTGCAC 2 cut(s) 834, 1311
AlwI GGATC 8 cut(s) 929, 942, 1088, 1433, 1769, 1822, 1981, 1992
AlwNI CAGNNNCTG 2 cut(s) 668, 1964
Ama87I CYCGRG 2 cut(s) 27, 1850
AoxI GGCC 6 cut(s) 105, 475, 533, 1731, 1763, 1819
ApaLI GTGCAC 2 cut(s) 834, 1311
ApeKI GCWGC 8 cut(s) 344, 713, 851, 992, 1058, 1583, 1679, 1782
ApoI RAATTY 7 cut(s) 528, 614, 923, 1126, 1522, 1575, 1802
Asp700I GAANNNNTTC 2 cut(s) 1139, 1299
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 7 cut(s) 94, 569, 1007, 1072, 1517, 1764, 1961
AsuC2I CCSGG 2 cut(s) 1851, 1852
AsuHPI GGTGA 2 cut(s) 245, 1811
AvaI CYCGRG 2 cut(s) 27, 1850
AvaII GGWCC 6 cut(s) 94, 569, 1007, 1072, 1517, 1961
BaeGI GKGCMC 3 cut(s) 838, 1315, 1564
BaeI ACNNNNGTAYC 2 cut(s) 492, 525
BamHI GGATCC 1 cut(s) 934
BanII GRGCYC 2 cut(s) 121, 646
BauI CACGAG 1 cut(s) 2217
BbrPI CACGTG 1 cut(s) 1514
BbsI GAAGAC 1 cut(s) 2177
Bbv12I GWGCWC 4 cut(s) 121, 646, 838, 1315
BbvI GCAGC 8 cut(s) 331, 725, 838, 979, 1070, 1570, 1666, 1794
BccI CCATC 6 cut(s) 116, 224, 264, 869, 1996, 2240
BceAI ACGGC 3 cut(s) 120, 229, 726
BcgI CGANNNNNNTGC 2 cut(s) 851, 885
BciT130I CCWGG 7 cut(s) 284, 362, 819, 848, 1238, 1566, 1617
BciVI GTATCC 1 cut(s) 969
BcnI CCSGG 2 cut(s) 1851, 1852
BcoDI GTCTC 5 cut(s) 12, 32, 255, 403, 1728
BfaI CTAG 2 cut(s) 1250, 2292
BfmI CTRYAG 2 cut(s) 269, 1956
BfuI GTATCC 1 cut(s) 969
BglI GCCNNNNNGGC 1 cut(s) 710
BglII AGATCT 1 cut(s) 440
Bme1390I CCNGG 9 cut(s) 284, 362, 819, 848, 1238, 1566, 1617, 1851, 1852
Bme18I GGWCC 6 cut(s) 94, 569, 1007, 1072, 1517, 1961
BmeT110I CYCGRG 2 cut(s) 27, 1850
BmgT120I GGNCC 7 cut(s) 94, 569, 1007, 1072, 1517, 1764, 1961
BmiI GGNNCC 5 cut(s) 6, 694, 936, 1657, 2008
BmrFI CCNGG 9 cut(s) 284, 362, 819, 848, 1238, 1566, 1617, 1851, 1852
BmrI ACTGGG 1 cut(s) 1325
BmsI GCATC 5 cut(s) 853, 1342, 1855, 1908, 2231
BmuI ACTGGG 1 cut(s) 1325
BoxI GACNNNNGTC 2 cut(s) 684, 1245
BpiI GAAGAC 1 cut(s) 2177
BpmI CTGGAG 1 cut(s) 1638
BpuMI CCSGG 2 cut(s) 1851, 1852
BsaAI YACGTR 1 cut(s) 1514
BsaBI GATNNNNATC 1 cut(s) 933
BsaI GGTCTC 1 cut(s) 1728
BsaWI WCCGGW 1 cut(s) 1013
BsaXI ACNNNNNCTCC 4 cut(s) 1412, 1442, 1998, 2028
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 103, 585, 1376, 1970, 2002, 2003
Bse1I ACTGG 4 cut(s) 756, 1320, 1801, 2238
Bse3DI GCAATG 5 cut(s) 487, 969, 1792, 2028, 2077
Bse8I GATNNNNATC 1 cut(s) 933
BseBI CCWGG 7 cut(s) 284, 362, 819, 848, 1238, 1566, 1617
BseGI GGATG 4 cut(s) 108, 150, 682, 1095
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 7 cut(s) 14, 103, 585, 1376, 1970, 2002, 2003
BseMI GCAATG 5 cut(s) 487, 969, 1792, 2028, 2077
BseMII CTCAG 2 cut(s) 152, 534
BseNI ACTGG 4 cut(s) 756, 1320, 1801, 2238
BseRI GAGGAG 5 cut(s) 77, 80, 786, 789, 2180
BseSI GKGCMC 3 cut(s) 838, 1315, 1564
BseXI GCAGC 8 cut(s) 331, 725, 838, 979, 1070, 1570, 1666, 1794
BseYI CCCAGC 1 cut(s) 1778
BsgI GTGCAG 1 cut(s) 1494
Bsh1236I CGCG 2 cut(s) 72, 1446
BshFI GGCC 6 cut(s) 107, 477, 535, 1733, 1765, 1821
BsiHKAI GWGCWC 4 cut(s) 121, 646, 838, 1315
BsiHKCI CYCGRG 2 cut(s) 27, 1850
BsiSI CCGG 3 cut(s) 1014, 1851, 2135
BslFI GGGAC 3 cut(s) 461, 1168, 1472
BslI CCNNNNNNNGG 7 cut(s) 14, 103, 585, 1376, 1970, 2002, 2003
BsmAI GTCTC 5 cut(s) 12, 32, 255, 403, 1728
BsmBI CGTCTC 3 cut(s) 12, 32, 255
BsmFI GGGAC 3 cut(s) 461, 1168, 1472
BsnI GGCC 6 cut(s) 107, 477, 535, 1733, 1765, 1821
Bso31I GGTCTC 1 cut(s) 1728
BsoBI CYCGRG 2 cut(s) 27, 1850
Bsp1286I GDGCHC 5 cut(s) 121, 646, 838, 1315, 1564
Bsp1407I TGTACA 1 cut(s) 2280
Bsp143I GATC 8 cut(s) 440, 934, 1080, 1438, 1774, 1814, 1973, 1984
Bsp19I CCATGG 2 cut(s) 112, 1662
BspACI CCGC 7 cut(s) 77, 478, 798, 995, 1214, 1730, 2019
BspANI GGCC 6 cut(s) 107, 477, 535, 1733, 1765, 1821
BspCNI CTCAG 2 cut(s) 153, 535
BspFNI CGCG 2 cut(s) 72, 1446
BspLI GGNNCC 5 cut(s) 6, 694, 936, 1657, 2008
BspPI GGATC 8 cut(s) 929, 942, 1088, 1433, 1769, 1822, 1981, 1992
BspQI GCTCTTC 1 cut(s) 126
BspTNI GGTCTC 1 cut(s) 1728
BsrDI GCAATG 5 cut(s) 487, 969, 1792, 2028, 2077
BsrGI TGTACA 1 cut(s) 2280
BsrI ACTGG 4 cut(s) 756, 1320, 1801, 2238
BssMI GATC 8 cut(s) 440, 934, 1080, 1438, 1774, 1814, 1973, 1984
BssSI CACGAG 1 cut(s) 2217
BssT1I CCWWGG 4 cut(s) 112, 1040, 1099, 1662
Bst2BI CACGAG 1 cut(s) 2217
Bst2UI CCWGG 7 cut(s) 284, 362, 819, 848, 1238, 1566, 1617
Bst6I CTCTTC 1 cut(s) 126
BstAPI GCANNNNNTGC 2 cut(s) 722, 794
BstAUI TGTACA 1 cut(s) 2280
BstBAI YACGTR 1 cut(s) 1514
BstC8I GCNNGC 4 cut(s) 57, 997, 1378, 1669
BstDEI CTNAG 4 cut(s) 34, 161, 260, 543
BstDSI CCRYGG 2 cut(s) 112, 1662
BstF5I GGATG 4 cut(s) 108, 150, 682, 1095
BstFNI CGCG 2 cut(s) 72, 1446
BstHHI GCGC 1 cut(s) 72
BstKTI GATC 8 cut(s) 443, 937, 1083, 1441, 1777, 1817, 1976, 1987
BstMAI GTCTC 5 cut(s) 12, 32, 255, 403, 1728
BstMBI GATC 8 cut(s) 440, 934, 1080, 1438, 1774, 1814, 1973, 1984
BstMWI GCNNNNNNNGC 7 cut(s) 56, 710, 722, 794, 902, 1490, 1688
BstNI CCWGG 7 cut(s) 284, 362, 819, 848, 1238, 1566, 1617
BstNSI RCATGY 2 cut(s) 992, 1953
BstPAI GACNNNNGTC 2 cut(s) 684, 1245
BstSCI CCNGG 9 cut(s) 282, 360, 817, 846, 1236, 1564, 1615, 1849, 1850
BstSFI CTRYAG 2 cut(s) 269, 1956
BstSLI GKGCMC 3 cut(s) 838, 1315, 1564
BstUI CGCG 2 cut(s) 72, 1446
BstV1I GCAGC 8 cut(s) 331, 725, 838, 979, 1070, 1570, 1666, 1794
BstV2I GAAGAC 1 cut(s) 2177
BstX2I RGATCY 4 cut(s) 440, 934, 1438, 1973
BstXI CCANNNNNNTGG 1 cut(s) 1682
BstYI RGATCY 4 cut(s) 440, 934, 1438, 1973
BsuI GTATCC 1 cut(s) 969
BsuRI GGCC 6 cut(s) 107, 477, 535, 1733, 1765, 1821
BtgI CCRYGG 2 cut(s) 112, 1662
BtsCI GGATG 4 cut(s) 108, 150, 682, 1095
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 6 cut(s) 354, 529, 699, 1313, 1794, 2231
Cac8I GCNNGC 4 cut(s) 57, 997, 1378, 1669
CaiI CAGNNNCTG 2 cut(s) 668, 1964
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 7 cut(s) 94, 569, 1007, 1072, 1517, 1764, 1961
Cfr9I CCCGGG 1 cut(s) 1850
CpoI CGGWCCG 1 cut(s) 94
Csp6I GTAC 7 cut(s) 211, 238, 813, 951, 1639, 2250, 2281
CspCI CAANNNNNGTGG 2 cut(s) 1700, 1735
CspI CGGWCCG 1 cut(s) 94
CviQI GTAC 7 cut(s) 211, 238, 813, 951, 1639, 2250, 2281
DdeI CTNAG 4 cut(s) 34, 161, 260, 543
DpnI GATC 8 cut(s) 442, 936, 1082, 1440, 1776, 1816, 1975, 1986
DpnII GATC 8 cut(s) 440, 934, 1080, 1438, 1774, 1814, 1973, 1984
DraI TTTAAA 1 cut(s) 2059
DraIII CACNNNGTG 1 cut(s) 828
EaeI YGGCCR 3 cut(s) 105, 475, 1731
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 2 cut(s) 119, 644
Eco130I CCWWGG 4 cut(s) 112, 1040, 1099, 1662
Eco24I GRGCYC 2 cut(s) 121, 646
Eco31I GGTCTC 1 cut(s) 1728
Eco47I GGWCC 6 cut(s) 94, 569, 1007, 1072, 1517, 1961
Eco53kI GAGCTC 2 cut(s) 119, 644
Eco57I CTGAAG 1 cut(s) 882
Eco72I CACGTG 1 cut(s) 1514
Eco88I CYCGRG 2 cut(s) 27, 1850
EcoICRI GAGCTC 2 cut(s) 119, 644
EcoO109I RGGNCCY 1 cut(s) 1961
EcoRI GAATTC 3 cut(s) 923, 1126, 1802
EcoRII CCWGG 7 cut(s) 282, 360, 817, 846, 1236, 1564, 1615
EcoT14I CCWWGG 4 cut(s) 112, 1040, 1099, 1662
EcoT38I GRGCYC 2 cut(s) 121, 646
ErhI CCWWGG 4 cut(s) 112, 1040, 1099, 1662
Esp3I CGTCTC 3 cut(s) 12, 32, 255
FaqI GGGAC 3 cut(s) 461, 1168, 1472
FauI CCCGC 2 cut(s) 988, 1207
FokI GGATG 4 cut(s) 95, 137, 689, 1082
FriOI GRGCYC 2 cut(s) 121, 646
FspBI CTAG 2 cut(s) 1250, 2292
GlaI GCGC 1 cut(s) 71
GsaI CCCAGC 1 cut(s) 1782
GsuI CTGGAG 1 cut(s) 1638
HaeIII GGCC 6 cut(s) 107, 477, 535, 1733, 1765, 1821
HapII CCGG 3 cut(s) 1014, 1851, 2135
HhaI GCGC 1 cut(s) 72
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HindIII AAGCTT 2 cut(s) 632, 739
HinfI GANTC 6 cut(s) 351, 459, 539, 665, 879, 2213
HpaII CCGG 3 cut(s) 1014, 1851, 2135
HphI GGTGA 2 cut(s) 245, 1811
Hpy188I TCNGA 9 cut(s) 94, 162, 229, 544, 1080, 1170, 1980, 2245, 2271
Hpy188III TCNNGA 7 cut(s) 296, 1292, 1653, 1935, 1964, 2193, 2217
Hpy99I CGWCG 4 cut(s) 46, 191, 250, 1080
HpyCH4IV ACGT 2 cut(s) 419, 1513
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 710, 722, 794, 902, 1490, 1688
HpyF3I CTNAG 4 cut(s) 34, 161, 260, 543
HpySE526I ACGT 2 cut(s) 419, 1513
HspAI GCGC 1 cut(s) 70
Kzo9I GATC 8 cut(s) 440, 934, 1080, 1438, 1774, 1814, 1973, 1984
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 6 cut(s) 4, 67, 116, 641, 1603, 2006
Lsp1109I GCAGC 8 cut(s) 331, 725, 838, 979, 1070, 1570, 1666, 1794
LweI GCATC 5 cut(s) 853, 1342, 1855, 1908, 2231
MaeI CTAG 2 cut(s) 1250, 2292
MaeII ACGT 2 cut(s) 419, 1513
MaeIII GTNAC 7 cut(s) 219, 317, 686, 760, 2014, 2038, 2182
MalI GATC 8 cut(s) 442, 936, 1082, 1440, 1776, 1816, 1975, 1986
MboI GATC 8 cut(s) 440, 934, 1080, 1438, 1774, 1814, 1973, 1984
MboII GAAGA 4 cut(s) 113, 1889, 2064, 2182
MfeI CAATTG 2 cut(s) 867, 945
MflI RGATCY 4 cut(s) 440, 934, 1438, 1973
MhlI GDGCHC 5 cut(s) 121, 646, 838, 1315, 1564
MlyI GAGTC 3 cut(s) 345, 674, 888
MmeI TCCRAC 1 cut(s) 1058
MroXI GAANNNNTTC 2 cut(s) 1139, 1299
MspI CCGG 3 cut(s) 1014, 1851, 2135
MspR9I CCNGG 9 cut(s) 284, 362, 819, 848, 1238, 1566, 1617, 1851, 1852
MunI CAATTG 2 cut(s) 867, 945
MvaI CCWGG 7 cut(s) 284, 362, 819, 848, 1238, 1566, 1617
MvnI CGCG 2 cut(s) 72, 1446
MwoI GCNNNNNNNGC 7 cut(s) 56, 710, 722, 794, 902, 1490, 1688
NciI CCSGG 2 cut(s) 1851, 1852
NcoI CCATGG 2 cut(s) 112, 1662
NdeII GATC 8 cut(s) 440, 934, 1080, 1438, 1774, 1814, 1973, 1984
NlaIV GGNNCC 5 cut(s) 6, 694, 936, 1657, 2008
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 3 cut(s) 219, 686, 2182
NspI RCATGY 2 cut(s) 992, 1953
PasI CCCWGGG 1 cut(s) 1565
PciSI GCTCTTC 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PdmI GAANNNNTTC 2 cut(s) 1139, 1299
PfeI GAWTC 3 cut(s) 459, 539, 2213
PflFI GACNNNGTC 1 cut(s) 1091
PflMI CCANNNNNTGG 2 cut(s) 585, 1376
PfoI TCCNGGA 2 cut(s) 282, 1615
PleI GAGTC 3 cut(s) 345, 673, 887
PmaCI CACGTG 1 cut(s) 1514
PmlI CACGTG 1 cut(s) 1514
PpsI GAGTC 3 cut(s) 345, 673, 887
Ppu21I YACGTR 1 cut(s) 1514
PpuMI RGGWCCY 1 cut(s) 1961
PshAI GACNNNNGTC 2 cut(s) 684, 1245
Psp124BI GAGCTC 2 cut(s) 121, 646
Psp1406I AACGTT 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 1961
Psp6I CCWGG 7 cut(s) 282, 360, 817, 846, 1236, 1564, 1615
PspCI CACGTG 1 cut(s) 1514
PspFI CCCAGC 1 cut(s) 1778
PspGI CCWGG 7 cut(s) 282, 360, 817, 846, 1236, 1564, 1615
PspN4I GGNNCC 5 cut(s) 6, 694, 936, 1657, 2008
PspPI GGNCC 7 cut(s) 94, 569, 1007, 1072, 1517, 1764, 1961
PspPPI RGGWCCY 1 cut(s) 1961
PstNI CAGNNNCTG 2 cut(s) 668, 1964
PsuI RGATCY 4 cut(s) 440, 934, 1438, 1973
PsyI GACNNNGTC 1 cut(s) 1091
RsaI GTAC 7 cut(s) 212, 239, 814, 952, 1640, 2251, 2282
RsaNI GTAC 7 cut(s) 211, 238, 813, 951, 1639, 2250, 2281
Rsr2I CGGWCCG 1 cut(s) 94
RsrII CGGWCCG 1 cut(s) 94
SacI GAGCTC 2 cut(s) 121, 646
SapI GCTCTTC 1 cut(s) 126
Sau3AI GATC 8 cut(s) 440, 934, 1080, 1438, 1774, 1814, 1973, 1984
Sau96I GGNCC 7 cut(s) 94, 569, 1007, 1072, 1517, 1764, 1961
SchI GAGTC 3 cut(s) 345, 674, 888
ScrFI CCNGG 9 cut(s) 284, 362, 819, 848, 1238, 1566, 1617, 1851, 1852
SduI GDGCHC 5 cut(s) 121, 646, 838, 1315, 1564
SfaNI GCATC 5 cut(s) 853, 1342, 1855, 1908, 2231
SfcI CTRYAG 2 cut(s) 269, 1956
SinI GGWCC 6 cut(s) 94, 569, 1007, 1072, 1517, 1961
SmaI CCCGGG 1 cut(s) 1852
SsiI CCGC 7 cut(s) 77, 478, 798, 995, 1214, 1730, 2019
SspMI CTAG 2 cut(s) 1250, 2292
SstI GAGCTC 2 cut(s) 121, 646
StyD4I CCNGG 9 cut(s) 282, 360, 817, 846, 1236, 1564, 1615, 1849, 1850
StyI CCWWGG 4 cut(s) 112, 1040, 1099, 1662
TaiI ACGT 2 cut(s) 422, 1516
TaqI TCGA 5 cut(s) 882, 927, 1339, 2000, 2118
TaqII GACCGA 1 cut(s) 111
TatI WGTACW 4 cut(s) 210, 237, 1638, 2280
TauI GCSGC 3 cut(s) 480, 800, 1733
TfiI GAWTC 3 cut(s) 459, 539, 2213
TscAI CASTG 6 cut(s) 361, 529, 706, 1320, 1801, 2238
TseFI GTSAC 3 cut(s) 219, 686, 2182
TseI GCWGC 8 cut(s) 344, 713, 851, 992, 1058, 1583, 1679, 1782
Tsp45I GTSAC 3 cut(s) 219, 686, 2182
TspDTI ATGAA 6 cut(s) 401, 442, 1491, 1751, 1784, 1926
TspGWI ACGGA 1 cut(s) 705
TspMI CCCGGG 1 cut(s) 1850
TspRI CASTG 6 cut(s) 361, 529, 706, 1320, 1801, 2238
Tth111I GACNNNGTC 1 cut(s) 1091
Van91I CCANNNNNTGG 2 cut(s) 585, 1376
VneI GTGCAC 2 cut(s) 834, 1311
VpaK11BI GGWCC 6 cut(s) 94, 569, 1007, 1072, 1517, 1961
XapI RAATTY 7 cut(s) 528, 614, 923, 1126, 1522, 1575, 1802
XceI RCATGY 2 cut(s) 992, 1953
XcmI CCANNNNNNNNNTGG 1 cut(s) 582
XmaI CCCGGG 1 cut(s) 1850
XmnI GAANNNNTTC 2 cut(s) 1139, 1299
XspI CTAG 2 cut(s) 1250, 2292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.