RLG00000035888

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000257
Physical Location & Seq
Forward (+)
73132219 .. 73135641
3423 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035888

Sequence Viewer

Length: 2202 bp
ATGGCTAAGCATGGAGCTCTTCTCTTTTATCACACCTTGTCAATTCTCATCCTCACTCTGAGTTTGTTATGCAGAGCCACTTACGACGATAGAAAGGTCTATATTGTGTACTTGGGGTCACTTTCTGATGGGTTGTACTCGCCGTCATCTCACCATCTTAGTATTCTACAGAGAGTTGTCCAGGACAGGTTTTCTCCTGAAAATGTTTTGGTAAGAAGTTACAAGAGGAGTTTCAATGGATTTGCTGCCAGACTCACTGACCAGGAAAGAGAGAAACTTTCTAAAATGAAGGAAGTAGTCTCTGTCTTTCCAAGCAGAACATTGCAACTTCAAACAACAAGATCTTGGGACTTCATGGGATTCAATGAGAAAATTGGTCGAAATGCCACAGTTGAGAGTGATATTATTGTGGGTGTCATTGATAGTGGAATTTGGCCTGAATCTGAGAGTTTTAAGGATGAAGGTTTTGGTCCTCCACCAAAGAAGTGGAAAGGTGCTTGTGAAGGGGGCAAAAATTTTACTTGCAACAACAAGCTTATTGGAGCTCGGTATTACTCATCAACAGAGTCTGCAAGGGATGACACAGGTCACGGAACCCACACTGCCTCAACGGCAGCAGGAAATGCTGTAAGAGATGTAAGCTTTTATGGACTCGCACAAGGTACTGCAAGAGGAGGAGTTCCCTCTGCAAGAATTGCAGCATACAAAGTCTGTACCCCGGGCACTTTGTGTAATGCACATGATACCCTGGCTGCTTTTGACGATGCAATTGCTGATGGAGTCGATATTATTACAGCTTCAGTTGCATTTAGTATGATACAAGAATTCGATGTGGATCCTCTTGCAATTGGTACTTTTCACGCAATGGAGAAAGGGATACTAACCTCACATGCTGCAGGCAACAATGGACCTTCCGGTGCTACTATATCAAGTGTAGCACCTTGGGTGCTTACAGTTGCAGCAAGTAGCATGGACCGTCGGATCATTGACAAGGTTATCCTTGGAAATGGAAGGACAGTAATGGGGAATTCTGTGAACTCTTTCACATTAAATGGTACAAGTTTTCCTCTGATACATGGAAAAAATGCTTCAAAGAATTGCACAGAAACAAGTGCGGGAGGTTGTGAACAAGGTTGCCTGGACAGTAGTCTAGTGAAGGGAAAGGTTGTGCTATGTGATAAATATGGACTTCTTGAAGCATTTCAGTCTGGTGCACTGGGTTCAATTTTACCCAATTTTCGAGCTGTTGATGATGCCTCTTATGTTCTCCCATTAGCTGGCACGACTATAAACAACGAAAACTATAATGTAGTCATGTCCTACATGAATTCCACCAGAGATCCTCGCGTGAACATATTAAAAAGTGAAGTCATAAAAAATCCTGCTGCACCTGCTGTTCCTTCATTCTCTGCACGTGGACCGAATTTAATTTTACCTGATATTATCAAGCCAGATATTAGTGCCCCAGGGATAGAAATTCTGGCTGCATTTTCGTCTGCTCCTATCTCAACCACTCCTGGAGATATGAGGCGTGTCAAGTACAATATACAATCTGGAACCTCCATGGCTTGCCCCCATGCTGCTGGTGTGGCTACCTATGTTAAAACCTTCCACCCTGATTGGTCTCCCGCGGCCATCAAATCATCTATTATGACTACTGCTTGGCCCATGAATGATCCCAGCAGCAATGTTTCCACTGGTGAATTTGCTTATGGATCAGGCCATATCAATCCATTAAAAGCTATTGACCCCGGGCTTGTGTATGATGCTTCCAAAGAAGATTACATAAAGTTGCTGTGCATCAGATATGATGAAAGCAGAGTTAGACTTGTATCAGGAGATAACAGCACATGCCCTACAGGTCCTGACAAGGGATCTCCGAAGGATCATAATTACCCTTCGATGGGAGCTAAAATTACCGCAATGCAACCATTTACAGTTACATTTAATAGAAGAGTTAAAAATGTTGGCAATGCAAACTCCACTTATGTAGCCAAAATCTCCCCCAATTCCAAACTCGAGATTAAAGTGGTGCCGGAAGTCCTATCCTTCAAGTCCTTGAATGAGGAGAAGACTTTTAATGTGACTGTTTCTGGAAGCAATTTGCCTTTTGAATCACGAGCATCTGCTTCACTGGTTTGGTCTGATGGTACTCATGGCGTTAGAAGTCCGATTGTTGTGTACACCGCGCCTAGAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

734

Amino Acids

78.67

Weight (kDa)

8.53

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 111 1.5e-17 Peptidase inhibitor I9
Peptidase_S8 PF00082 133 - 572 3.7e-41 Subtilase family
fn3_6 PF17766 629 - 727 1e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000257)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46840 AT3G46850 AT5G58820 AT5G58830 AT5G58840 AT5G58840 AT5G58840 AT5G59090 AT5G59090 AT5G59090 AT5G59100 AT5G59120 AT5G59130 AT5G59130 AT5G59130 AT5G59130 AT5G59190
fragaria_vesca FvH4_3g37170 FvH4_7g02830 FvH4_7g02840
malus_domestica MD02G1288900.v1.1 MD02G1289100.v1.1 MD02G1289300.v1.1 MD02G1289400.v1.1 MD02G1289500.v1.1 MD03G1083800.v1.1 MD03G1084000.v1.1 MD07G1037500.v1.1 MD07G1037600.v1.1 MD07G1037700.v1.1 MD07G1039300.v1.1 MD11G1091900.v1.1 MD11G1092000.v1.1
prunus_persica Prupe.2G032800_v2.0.a1 Prupe.2G033000_v2.0.a1 Prupe.2G033100_v2.0.a1 Prupe.2G035100_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067600_v2.0.a1 Prupe.6G067700_v2.0.a1 Prupe.6G067800_v2.0.a1
pyrus_communis pycom02g24440 pycom02g24470 pycom02g24480 pycom02g24500 pycom02g24510 pycom03g06650 pycom07g02730 pycom11g07710 pycom11g07720
rosa_chinensis RchiOBHm_Chr1g0323281 RchiOBHm_Chr1g0323291 RchiOBHm_Chr1g0323311 RchiOBHm_Chr1g0323341 RchiOBHm_Chr1g0323351 RchiOBHm_Chr1g0323371 RchiOBHm_Chr1g0323431 RchiOBHm_Chr1g0323461 RchiOBHm_Chr1g0324031 RchiOBHm_Chr5g0066891 RchiOBHm_Chr5g0066901
rosa_laevigata RLG00000030004 RLG00000030235 RLG00000030328 RLG00000030329 RLG00000030334 RLG00000030336 RLG00000030337 RLG00000030338 RLG00000030339 RLG00000030340 RLG00000035885 RLG00000035886 RLG00000035888 RLG00000035890
rosa_multiflora Rmu_co8481685.1_g000001 Rmu_co8485349.1_g000001 Rmu_sc0000605.1_g000061 Rmu_sc0000804.1_g000004 Rmu_sc0000804.1_g000008 Rmu_sc0002263.1_g000074 Rmu_sc0002263.1_g000076 Rmu_sc0002263.1_g000077 Rmu_sc0003418.1_g000005 Rmu_sc0003418.1_g000007 Rmu_sc0003418.1_g000008 Rmu_sc0008378.1_g000013 Rmu_sc0010151.1_g000012
rosa_roxburghii Rroxscaffold_1G00014220 Rroxscaffold_1G00014230 Rroxscaffold_4G00325970 Rroxscaffold_4G00326160 Rroxscaffold_4G00326570 Rroxscaffold_4G00326580 Rroxscaffold_4G00326650 Rroxscaffold_4G00326670 Rroxscaffold_4G00326680 Rroxscaffold_4G00326700
rosa_rugosa Rorug01G0037300 Rorug01G0037400 Rorug01G0037600 Rorug01G0037700 Rorug01G0037800 Rorug01G0037800 Rorug01G0042100 Rorug01G0065000 Rorug05G0379300 Rorug05G0379400
rosa_samantha Rh1AG054700 Rh1AG054900 Rh1AG055000 Rh1AG055200 Rh1AG055300 Rh1BG047500 Rh1CG055800 Rh1CG056000 Rh1CG061400 Rh1DG059900 Rh1DG060200 Rh5AG439100 Rh5AG439200 Rh5BG455900 Rh5CG478000 Rh5CG478300
rosa_wichuraiana Rw1G004750 Rw1G004760 Rw1G004770 Rw1G004790 Rw1G005080 Rw1G006510 Rw5G041060 Rw5G041070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1399
Acc36I ACCTGC 1 cut(s) 1399
AccB1I GGYRCC 1 cut(s) 2032
AccB7I CCANNNNNTGG 1 cut(s) 1277
AccII CGCG 3 cut(s) 1347, 1631, 2189
AciI CCGC 5 cut(s) 1115, 1629, 1631, 1920, 2187
AclWI GGATC 8 cut(s) 830, 843, 989, 1334, 1670, 1723, 1882, 1893
AcoI YGGCCR 1 cut(s) 1632
AcsI RAATTY 8 cut(s) 429, 514, 824, 1027, 1327, 1423, 1476, 1703
AcuI CTGAAG 1 cut(s) 783
AcvI CACGTG 1 cut(s) 1415
AdeI CACNNNGTG 1 cut(s) 729
AfaI GTAC 9 cut(s) 110, 137, 664, 715, 853, 1057, 1541, 2152, 2183
AfiI CCNNNNNNNGG 4 cut(s) 1277, 1871, 1903, 1904
AgsI TTSAA 9 cut(s) 235, 332, 364, 1092, 1196, 1224, 2053, 2062, 2114
AjnI CCWGG 6 cut(s) 180, 261, 747, 1137, 1465, 1516
AluBI AGCT 9 cut(s) 17, 535, 545, 642, 797, 1244, 1277, 1742, 1910
AluI AGCT 9 cut(s) 17, 535, 545, 642, 797, 1244, 1277, 1742, 1910
Alw21I GWGCWC 3 cut(s) 19, 547, 1216
Alw26I GTCTC 2 cut(s) 304, 1629
Alw44I GTGCAC 1 cut(s) 1212
AlwI GGATC 8 cut(s) 830, 843, 989, 1334, 1670, 1723, 1882, 1893
AlwNI CAGNNNCTG 2 cut(s) 569, 1865
Ama87I CYCGRG 3 cut(s) 718, 1751, 2018
AoxI GGCC 4 cut(s) 434, 1632, 1664, 1720
ApaLI GTGCAC 1 cut(s) 1212
ApoI RAATTY 8 cut(s) 429, 514, 824, 1027, 1327, 1423, 1476, 1703
Asp700I GAANNNNTTC 2 cut(s) 1040, 1200
AspLEI GCGC 1 cut(s) 2191
AspS9I GGNCC 6 cut(s) 470, 908, 973, 1418, 1665, 1862
AsuC2I CCSGG 4 cut(s) 719, 720, 1752, 1753
AsuHPI GGTGA 2 cut(s) 143, 1712
AvaI CYCGRG 3 cut(s) 718, 1751, 2018
AvaII GGWCC 5 cut(s) 470, 908, 973, 1418, 1862
BaeGI GKGCMC 3 cut(s) 725, 1216, 1465
BamHI GGATCC 1 cut(s) 835
BanI GGYRCC 1 cut(s) 2032
BanII GRGCYC 2 cut(s) 19, 547
BauI CACGAG 1 cut(s) 2118
BbrPI CACGTG 1 cut(s) 1415
BbsI GAAGAC 1 cut(s) 2078
Bbv12I GWGCWC 3 cut(s) 19, 547, 1216
BccI CCATC 6 cut(s) 122, 162, 770, 1643, 1897, 2141
BceAI ACGGC 2 cut(s) 127, 627
BcgI CGANNNNNNTGC 2 cut(s) 752, 786
BciT130I CCWGG 6 cut(s) 182, 263, 749, 1139, 1467, 1518
BciVI GTATCC 1 cut(s) 870
BcnI CCSGG 4 cut(s) 719, 720, 1752, 1753
BcoDI GTCTC 2 cut(s) 304, 1629
BfaI CTAG 2 cut(s) 1151, 2193
BfmI CTRYAG 3 cut(s) 167, 894, 1857
BfuAI ACCTGC 1 cut(s) 1399
BfuI GTATCC 1 cut(s) 870
BglI GCCNNNNNGGC 1 cut(s) 611
BglII AGATCT 1 cut(s) 341
BlpI GCTNAGC 1 cut(s) 6
Bme18I GGWCC 5 cut(s) 470, 908, 973, 1418, 1862
BmeT110I CYCGRG 3 cut(s) 718, 1751, 2018
BmgT120I GGNCC 6 cut(s) 470, 908, 973, 1418, 1665, 1862
BmiI GGNNCC 4 cut(s) 595, 837, 1558, 2034
BmrI ACTGGG 1 cut(s) 1226
BmsI GCATC 5 cut(s) 754, 1243, 1756, 1809, 2132
BmuI ACTGGG 1 cut(s) 1226
BoxI GACNNNNGTC 2 cut(s) 585, 1146
BpiI GAAGAC 1 cut(s) 2078
BplI GAGNNNNNCTC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 1539
Bpu1102I GCTNAGC 1 cut(s) 6
BpuMI CCSGG 4 cut(s) 719, 720, 1752, 1753
BsaAI YACGTR 1 cut(s) 1415
BsaBI GATNNNNATC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 1629
BsaWI WCCGGW 1 cut(s) 914
Bsc4I CCNNNNNNNGG 4 cut(s) 1277, 1871, 1903, 1904
Bse1I ACTGG 3 cut(s) 1221, 1702, 2139
Bse3DI GCAATG 5 cut(s) 320, 870, 1693, 1929, 1978
Bse8I GATNNNNATC 1 cut(s) 834
BseBI CCWGG 6 cut(s) 182, 263, 749, 1139, 1467, 1518
BseGI GGATG 3 cut(s) 48, 463, 583
BseJI GATNNNNATC 1 cut(s) 834
BseLI CCNNNNNNNGG 4 cut(s) 1277, 1871, 1903, 1904
BseMI GCAATG 5 cut(s) 320, 870, 1693, 1929, 1978
BseMII CTCAG 2 cut(s) 50, 435
BseNI ACTGG 3 cut(s) 1221, 1702, 2139
BseRI GAGGAG 4 cut(s) 241, 687, 690, 2081
BseSI GKGCMC 3 cut(s) 725, 1216, 1465
BseYI CCCAGC 1 cut(s) 1679
BsgI GTGCAG 2 cut(s) 1371, 1395
Bsh1236I CGCG 3 cut(s) 1347, 1631, 2189
BshFI GGCC 4 cut(s) 436, 1634, 1666, 1722
BshNI GGYRCC 1 cut(s) 2032
BsiHKAI GWGCWC 3 cut(s) 19, 547, 1216
BsiHKCI CYCGRG 3 cut(s) 718, 1751, 2018
BsiSI CCGG 4 cut(s) 719, 915, 1752, 2036
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 4 cut(s) 1277, 1871, 1903, 1904
BsmAI GTCTC 2 cut(s) 304, 1629
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 4 cut(s) 436, 1634, 1666, 1722
Bso31I GGTCTC 1 cut(s) 1629
BsoBI CYCGRG 3 cut(s) 718, 1751, 2018
Bsp1286I GDGCHC 5 cut(s) 19, 547, 725, 1216, 1465
Bsp1407I TGTACA 1 cut(s) 2181
Bsp143I GATC 8 cut(s) 341, 835, 981, 1339, 1675, 1715, 1874, 1885
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 1563
BspACI CCGC 5 cut(s) 1115, 1629, 1631, 1920, 2187
BspANI GGCC 4 cut(s) 436, 1634, 1666, 1722
BspCNI CTCAG 2 cut(s) 51, 436
BspFNI CGCG 3 cut(s) 1347, 1631, 2189
BspLI GGNNCC 4 cut(s) 595, 837, 1558, 2034
BspMAI CTGCAG 1 cut(s) 898
BspMI ACCTGC 1 cut(s) 1399
BspPI GGATC 8 cut(s) 830, 843, 989, 1334, 1670, 1723, 1882, 1893
BspQI GCTCTTC 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 2032
BspTNI GGTCTC 1 cut(s) 1629
BsrDI GCAATG 5 cut(s) 320, 870, 1693, 1929, 1978
BsrGI TGTACA 1 cut(s) 2181
BsrI ACTGG 3 cut(s) 1221, 1702, 2139
BssMI GATC 8 cut(s) 341, 835, 981, 1339, 1675, 1715, 1874, 1885
BssSI CACGAG 1 cut(s) 2118
BssT1I CCWWGG 3 cut(s) 941, 1000, 1563
Bst2BI CACGAG 1 cut(s) 2118
Bst2UI CCWGG 6 cut(s) 182, 263, 749, 1139, 1467, 1518
Bst4CI ACNGT 7 cut(s) 391, 955, 977, 1018, 1145, 1939, 2089
Bst6I CTCTTC 2 cut(s) 24, 1948
BstAPI GCANNNNNTGC 2 cut(s) 623, 695
BstAUI TGTACA 1 cut(s) 2181
BstBAI YACGTR 1 cut(s) 1415
BstC8I GCNNGC 3 cut(s) 898, 1279, 1570
BstDEI CTNAG 4 cut(s) 6, 59, 158, 444
BstDSI CCRYGG 2 cut(s) 1563, 1629
BstF5I GGATG 3 cut(s) 48, 463, 583
BstFNI CGCG 3 cut(s) 1347, 1631, 2189
BstHHI GCGC 1 cut(s) 2191
BstKTI GATC 8 cut(s) 344, 838, 984, 1342, 1678, 1718, 1877, 1888
BstMAI GTCTC 2 cut(s) 304, 1629
BstMBI GATC 8 cut(s) 341, 835, 981, 1339, 1675, 1715, 1874, 1885
BstMWI GCNNNNNNNGC 7 cut(s) 611, 623, 695, 803, 1391, 1589, 2195
BstNI CCWGG 6 cut(s) 182, 263, 749, 1139, 1467, 1518
BstNSI RCATGY 2 cut(s) 893, 1854
BstPAI GACNNNNGTC 2 cut(s) 585, 1146
BstSFI CTRYAG 3 cut(s) 167, 894, 1857
BstSLI GKGCMC 3 cut(s) 725, 1216, 1465
BstUI CGCG 3 cut(s) 1347, 1631, 2189
BstV2I GAAGAC 1 cut(s) 2078
BstX2I RGATCY 4 cut(s) 341, 835, 1339, 1874
BstXI CCANNNNNNTGG 2 cut(s) 486, 1583
BstYI RGATCY 4 cut(s) 341, 835, 1339, 1874
BsuI GTATCC 1 cut(s) 870
BsuRI GGCC 4 cut(s) 436, 1634, 1666, 1722
BtgI CCRYGG 2 cut(s) 1563, 1629
BtsCI GGATG 3 cut(s) 48, 463, 583
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 5 cut(s) 255, 600, 1214, 1695, 2132
BveI ACCTGC 1 cut(s) 1399
Cac8I GCNNGC 3 cut(s) 898, 1279, 1570
CaiI CAGNNNCTG 2 cut(s) 569, 1865
CfoI GCGC 1 cut(s) 2191
Cfr13I GGNCC 6 cut(s) 470, 908, 973, 1418, 1665, 1862
Cfr42I CCGCGG 1 cut(s) 1632
Cfr9I CCCGGG 2 cut(s) 718, 1751
Csp6I GTAC 9 cut(s) 109, 136, 663, 714, 852, 1056, 1540, 2151, 2182
CspCI CAANNNNNGTGG 2 cut(s) 1601, 1636
CviQI GTAC 9 cut(s) 109, 136, 663, 714, 852, 1056, 1540, 2151, 2182
DdeI CTNAG 4 cut(s) 6, 59, 158, 444
DpnI GATC 8 cut(s) 343, 837, 983, 1341, 1677, 1717, 1876, 1887
DpnII GATC 8 cut(s) 341, 835, 981, 1339, 1675, 1715, 1874, 1885
DraIII CACNNNGTG 1 cut(s) 729
EaeI YGGCCR 1 cut(s) 1632
Eam1104I CTCTTC 2 cut(s) 24, 1948
EarI CTCTTC 2 cut(s) 24, 1948
Ecl136II GAGCTC 2 cut(s) 17, 545
Eco130I CCWWGG 3 cut(s) 941, 1000, 1563
Eco24I GRGCYC 2 cut(s) 19, 547
Eco31I GGTCTC 1 cut(s) 1629
Eco47I GGWCC 5 cut(s) 470, 908, 973, 1418, 1862
Eco53kI GAGCTC 2 cut(s) 17, 545
Eco57I CTGAAG 1 cut(s) 783
Eco72I CACGTG 1 cut(s) 1415
Eco88I CYCGRG 3 cut(s) 718, 1751, 2018
EcoICRI GAGCTC 2 cut(s) 17, 545
EcoO109I RGGNCCY 1 cut(s) 1862
EcoRI GAATTC 3 cut(s) 824, 1027, 1327
EcoRII CCWGG 6 cut(s) 180, 261, 747, 1137, 1465, 1516
EcoT14I CCWWGG 3 cut(s) 941, 1000, 1563
EcoT38I GRGCYC 2 cut(s) 19, 547
ErhI CCWWGG 3 cut(s) 941, 1000, 1563
FaqI GGGAC 1 cut(s) 362
FauI CCCGC 2 cut(s) 1108, 1636
FokI GGATG 3 cut(s) 35, 470, 590
FriOI GRGCYC 2 cut(s) 19, 547
FspBI CTAG 2 cut(s) 1151, 2193
GlaI GCGC 1 cut(s) 2190
GsaI CCCAGC 1 cut(s) 1683
GsuI CTGGAG 1 cut(s) 1539
HaeIII GGCC 4 cut(s) 436, 1634, 1666, 1722
HapII CCGG 4 cut(s) 719, 915, 1752, 2036
HhaI GCGC 1 cut(s) 2191
Hin6I GCGC 1 cut(s) 2189
HinP1I GCGC 1 cut(s) 2189
HindIII AAGCTT 2 cut(s) 533, 640
HinfI GANTC 7 cut(s) 252, 360, 440, 566, 651, 780, 2114
HpaII CCGG 4 cut(s) 719, 915, 1752, 2036
HphI GGTGA 2 cut(s) 143, 1712
Hpy166II GTNNAC 8 cut(s) 109, 1036, 1127, 1214, 1351, 1418, 2182, 2184
Hpy188I TCNGA 9 cut(s) 60, 127, 445, 981, 1071, 1805, 1881, 2146, 2172
Hpy188III TCNNGA 8 cut(s) 197, 1193, 1554, 1836, 1865, 2020, 2094, 2118
Hpy8I GTNNAC 8 cut(s) 109, 1036, 1127, 1214, 1351, 1418, 2182, 2184
Hpy99I CGWCG 2 cut(s) 89, 981
HpyCH4III ACNGT 7 cut(s) 391, 955, 977, 1018, 1145, 1939, 2089
HpyCH4IV ACGT 1 cut(s) 1414
HpyF10VI GCNNNNNNNGC 7 cut(s) 611, 623, 695, 803, 1391, 1589, 2195
HpyF3I CTNAG 4 cut(s) 6, 59, 158, 444
HpySE526I ACGT 1 cut(s) 1414
HspAI GCGC 1 cut(s) 2189
KspI CCGCGG 1 cut(s) 1632
Kzo9I GATC 8 cut(s) 341, 835, 981, 1339, 1675, 1715, 1874, 1885
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 4 cut(s) 14, 542, 1504, 1907
LweI GCATC 5 cut(s) 754, 1243, 1756, 1809, 2132
MaeI CTAG 2 cut(s) 1151, 2193
MaeII ACGT 1 cut(s) 1414
MaeIII GTNAC 5 cut(s) 117, 218, 587, 1939, 2083
MalI GATC 8 cut(s) 343, 837, 983, 1341, 1677, 1717, 1876, 1887
MboI GATC 8 cut(s) 341, 835, 981, 1339, 1675, 1715, 1874, 1885
MboII GAAGA 4 cut(s) 11, 1790, 1965, 2083
MfeI CAATTG 2 cut(s) 768, 846
MflI RGATCY 4 cut(s) 341, 835, 1339, 1874
MhlI GDGCHC 5 cut(s) 19, 547, 725, 1216, 1465
MlyI GAGTC 4 cut(s) 246, 575, 645, 789
MmeI TCCRAC 1 cut(s) 959
MroXI GAANNNNTTC 2 cut(s) 1040, 1200
MspA1I CMGCKG 1 cut(s) 1631
MspI CCGG 4 cut(s) 719, 915, 1752, 2036
MunI CAATTG 2 cut(s) 768, 846
MvaI CCWGG 6 cut(s) 182, 263, 749, 1139, 1467, 1518
MvnI CGCG 3 cut(s) 1347, 1631, 2189
MwoI GCNNNNNNNGC 7 cut(s) 611, 623, 695, 803, 1391, 1589, 2195
NciI CCSGG 4 cut(s) 719, 720, 1752, 1753
NcoI CCATGG 1 cut(s) 1563
NdeII GATC 8 cut(s) 341, 835, 981, 1339, 1675, 1715, 1874, 1885
NlaIV GGNNCC 4 cut(s) 595, 837, 1558, 2034
NmuCI GTSAC 3 cut(s) 117, 587, 2083
NspI RCATGY 2 cut(s) 893, 1854
PaeR7I CTCGAG 1 cut(s) 2018
PaqCI CACCTGC 1 cut(s) 1399
PasI CCCWGGG 1 cut(s) 1466
PciSI GCTCTTC 1 cut(s) 24
PdmI GAANNNNTTC 2 cut(s) 1040, 1200
PfeI GAWTC 3 cut(s) 360, 440, 2114
PflMI CCANNNNNTGG 1 cut(s) 1277
PfoI TCCNGGA 2 cut(s) 180, 1516
PleI GAGTC 4 cut(s) 246, 574, 645, 788
PmaCI CACGTG 1 cut(s) 1415
PmlI CACGTG 1 cut(s) 1415
PpsI GAGTC 4 cut(s) 246, 574, 645, 788
Ppu21I YACGTR 1 cut(s) 1415
PpuMI RGGWCCY 1 cut(s) 1862
PshAI GACNNNNGTC 2 cut(s) 585, 1146
Psp124BI GAGCTC 2 cut(s) 19, 547
Psp5II RGGWCCY 1 cut(s) 1862
Psp6I CCWGG 6 cut(s) 180, 261, 747, 1137, 1465, 1516
PspCI CACGTG 1 cut(s) 1415
PspFI CCCAGC 1 cut(s) 1679
PspGI CCWGG 6 cut(s) 180, 261, 747, 1137, 1465, 1516
PspN4I GGNNCC 4 cut(s) 595, 837, 1558, 2034
PspPI GGNCC 6 cut(s) 470, 908, 973, 1418, 1665, 1862
PspPPI RGGWCCY 1 cut(s) 1862
PstI CTGCAG 1 cut(s) 898
PstNI CAGNNNCTG 2 cut(s) 569, 1865
PsuI RGATCY 4 cut(s) 341, 835, 1339, 1874
RsaI GTAC 9 cut(s) 110, 137, 664, 715, 853, 1057, 1541, 2152, 2183
RsaNI GTAC 9 cut(s) 109, 136, 663, 714, 852, 1056, 1540, 2151, 2182
SacI GAGCTC 2 cut(s) 19, 547
SacII CCGCGG 1 cut(s) 1632
SapI GCTCTTC 1 cut(s) 24
Sau3AI GATC 8 cut(s) 341, 835, 981, 1339, 1675, 1715, 1874, 1885
Sau96I GGNCC 6 cut(s) 470, 908, 973, 1418, 1665, 1862
SchI GAGTC 4 cut(s) 246, 575, 645, 789
SduI GDGCHC 5 cut(s) 19, 547, 725, 1216, 1465
SfaNI GCATC 5 cut(s) 754, 1243, 1756, 1809, 2132
SfcI CTRYAG 3 cut(s) 167, 894, 1857
Sfr274I CTCGAG 1 cut(s) 2018
Sfr303I CCGCGG 1 cut(s) 1632
SgrBI CCGCGG 1 cut(s) 1632
SinI GGWCC 5 cut(s) 470, 908, 973, 1418, 1862
SlaI CTCGAG 1 cut(s) 2018
SmaI CCCGGG 2 cut(s) 720, 1753
SmlI CTYRAG 1 cut(s) 2018
SmoI CTYRAG 1 cut(s) 2018
SsiI CCGC 5 cut(s) 1115, 1629, 1631, 1920, 2187
SspMI CTAG 2 cut(s) 1151, 2193
SstI GAGCTC 2 cut(s) 19, 547
StyI CCWWGG 3 cut(s) 941, 1000, 1563
TaaI ACNGT 7 cut(s) 391, 955, 977, 1018, 1145, 1939, 2089
TaiI ACGT 1 cut(s) 1417
TaqI TCGA 6 cut(s) 379, 783, 828, 1240, 1901, 2019
TaqII GACCGA 1 cut(s) 1435
TatI WGTACW 4 cut(s) 108, 135, 1539, 2181
TauI GCSGC 1 cut(s) 1634
TfiI GAWTC 3 cut(s) 360, 440, 2114
TscAI CASTG 5 cut(s) 262, 607, 1221, 1702, 2139
TseFI GTSAC 3 cut(s) 117, 587, 2083
Tsp45I GTSAC 3 cut(s) 117, 587, 2083
TspDTI ATGAA 7 cut(s) 302, 343, 474, 1340, 1392, 1685, 1827
TspGWI ACGGA 1 cut(s) 606
TspMI CCCGGG 2 cut(s) 718, 1751
TspRI CASTG 5 cut(s) 262, 607, 1221, 1702, 2139
Van91I CCANNNNNTGG 1 cut(s) 1277
VneI GTGCAC 1 cut(s) 1212
VpaK11BI GGWCC 5 cut(s) 470, 908, 973, 1418, 1862
XapI RAATTY 8 cut(s) 429, 514, 824, 1027, 1327, 1423, 1476, 1703
XceI RCATGY 2 cut(s) 893, 1854
XcmI CCANNNNNNNNNTGG 1 cut(s) 483
XhoI CTCGAG 1 cut(s) 2018
XmaI CCCGGG 2 cut(s) 718, 1751
XmnI GAANNNNTTC 2 cut(s) 1040, 1200
XspI CTAG 2 cut(s) 1151, 2193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.