cucumisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1584, 2843, 6107 |
| AarI | CACCTGC | 1 cut(s) | 2759 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 5719 |
| Acc36I | ACCTGC | 5 cut(s) | 2411, 2759, 4100, 4319, 4480 |
| AccB1I | GGYRCC | 4 cut(s) | 1738, 5188, 5869, 5906 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 4078 |
| AccBSI | CCGCTC | 1 cut(s) | 1721 |
| AccI | GTMKAC | 1 cut(s) | 3324 |
| AccII | CGCG | 7 cut(s) | 13, 1014, 2597, 2632, 3461, 3905, 6242 |
| AccIII | TCCGGA | 1 cut(s) | 5249 |
| AclI | AACGTT | 1 cut(s) | 1975 |
| AclWI | GGATC | 9 cut(s) | 2036, 2049, 2221, 3340, 4334, 5049, 5553, 5878, 5891 |
| AcoI | YGGCCR | 2 cut(s) | 1716, 3109 |
| AcuI | CTGAAG | 2 cut(s) | 1293, 3219 |
| AcyI | GRCGYC | 2 cut(s) | 1812, 5870 |
| AdeI | CACNNNGTG | 1 cut(s) | 4430 |
| AflIII | ACRYGT | 3 cut(s) | 11, 581, 1012 |
| AgeI | ACCGGT | 1 cut(s) | 6158 |
| AjnI | CCWGG | 4 cut(s) | 1478, 5655, 5673, 5902 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 685, 705, 717, 737 |
| AleI | CACNNNNGTG | 1 cut(s) | 2693 |
| AloI | GAACNNNNNNTCC | 6 cut(s) | 3338, 3370, 4261, 4293, 6221, 6253 |
| Alw21I | GWGCWC | 7 cut(s) | 1010, 1382, 2119, 3533, 3766, 4426, 6311 |
| Alw44I | GTGCAC | 3 cut(s) | 1006, 2115, 4422 |
| AlwI | GGATC | 9 cut(s) | 2036, 2049, 2221, 3340, 4334, 5049, 5553, 5878, 5891 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 2205, 6357 |
| Ama87I | CYCGRG | 3 cut(s) | 4608, 4660, 6203 |
| Aor13HI | TCCGGA | 1 cut(s) | 5249 |
| ApaLI | GTGCAC | 3 cut(s) | 1006, 2115, 4422 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 1158, 1190, 2168, 2200, 5733, 5765 |
| AseI | ATTAAT | 4 cut(s) | 176, 1557, 2868, 3086 |
| AsiGI | ACCGGT | 1 cut(s) | 6158 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 67, 191, 3005, 3062 |
| AspA2I | CCTAGG | 1 cut(s) | 2603 |
| AspLEI | GCGC | 7 cut(s) | 857, 2494, 2756, 3947, 4415, 5872, 6244 |
| AsuII | TTCGAA | 4 cut(s) | 1707, 3009, 3416, 5320 |
| AvaI | CYCGRG | 3 cut(s) | 4608, 4660, 6203 |
| AvaII | GGWCC | 3 cut(s) | 496, 3686, 5721 |
| AvrII | CCTAGG | 1 cut(s) | 2603 |
| AxyI | CCTNAGG | 2 cut(s) | 1207, 3342 |
| BaeGI | GKGCMC | 3 cut(s) | 1010, 2119, 4426 |
| BaeI | ACNNNNGTAYC | 3 cut(s) | 33, 5262, 5295 |
| BalI | TGGCCA | 1 cut(s) | 3111 |
| BamHI | GGATCC | 2 cut(s) | 2041, 5883 |
| BanI | GGYRCC | 4 cut(s) | 1738, 5188, 5869, 5906 |
| BanII | GRGCYC | 5 cut(s) | 386, 1305, 1382, 3766, 4136 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 3397, 3429 |
| BauI | CACGAG | 3 cut(s) | 1516, 4539, 5935 |
| BbsI | GAAGAC | 2 cut(s) | 4725, 5076 |
| Bbv12I | GWGCWC | 7 cut(s) | 1010, 1382, 2119, 3533, 3766, 4426, 6311 |
| BbvCI | CCTCAGC | 1 cut(s) | 3784 |
| BceAI | ACGGC | 6 cut(s) | 574, 640, 1155, 2991, 3277, 4872 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 4031, 4065, 5752, 5786 |
| BciT130I | CCWGG | 4 cut(s) | 1480, 5657, 5675, 5904 |
| BciVI | GTATCC | 1 cut(s) | 3159 |
| BclI | TGATCA | 3 cut(s) | 1018, 2718, 6138 |
| BfmI | CTRYAG | 7 cut(s) | 329, 360, 1642, 4053, 4107, 4473, 5761 |
| BfoI | RGCGCY | 1 cut(s) | 5873 |
| BfuAI | ACCTGC | 5 cut(s) | 2411, 2759, 4100, 4319, 4480 |
| BfuI | GTATCC | 1 cut(s) | 3159 |
| BglII | AGATCT | 4 cut(s) | 227, 3269, 3557, 4573 |
| BlnI | CCTAGG | 1 cut(s) | 2603 |
| BlpI | GCTNAGC | 2 cut(s) | 1722, 5609 |
| BmcAI | AGTACT | 1 cut(s) | 1841 |
| Bme18I | GGWCC | 3 cut(s) | 496, 3686, 5721 |
| BmeT110I | CYCGRG | 3 cut(s) | 4608, 4660, 6203 |
| BmrI | ACTGGG | 1 cut(s) | 3651 |
| BmuI | ACTGGG | 1 cut(s) | 3651 |
| BoxI | GACNNNNGTC | 1 cut(s) | 4232 |
| BpiI | GAAGAC | 2 cut(s) | 4725, 5076 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 3392, 3424 |
| BpmI | CTGGAG | 3 cut(s) | 2477, 4100, 4809 |
| Bpu10I | CCTNAGC | 2 cut(s) | 3784, 3873 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1722, 5609 |
| Bpu14I | TTCGAA | 4 cut(s) | 1707, 3009, 3416, 5320 |
| BpuEI | CTTGAG | 5 cut(s) | 2003, 2005, 2248, 5178, 6284 |
| Bsa29I | ATCGAT | 4 cut(s) | 3636, 3752, 5602, 5667 |
| BsaAI | YACGTR | 2 cut(s) | 5270, 5274 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2295 |
| BsaHI | GRCGYC | 2 cut(s) | 1812, 5870 |
| BsaI | GGTCTC | 2 cut(s) | 2825, 4827 |
| BsaWI | WCCGGW | 4 cut(s) | 2044, 4722, 5249, 6158 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 4508, 4538 |
| Bse118I | RCCGGY | 2 cut(s) | 1316, 6158 |
| Bse1I | ACTGG | 7 cut(s) | 1719, 3646, 4083, 4823, 4895, 5026, 5064 |
| Bse21I | CCTNAGG | 2 cut(s) | 1207, 3342 |
| Bse3DI | GCAATG | 2 cut(s) | 3622, 6325 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2295 |
| BseAI | TCCGGA | 1 cut(s) | 5249 |
| BseBI | CCWGG | 4 cut(s) | 1480, 5657, 5675, 5904 |
| BseCI | ATCGAT | 4 cut(s) | 3636, 3752, 5602, 5667 |
| BseJI | GATNNNNATC | 1 cut(s) | 2295 |
| BseMI | GCAATG | 2 cut(s) | 3622, 6325 |
| BseMII | CTCAG | 6 cut(s) | 1213, 1221, 1736, 3798, 3887, 6123 |
| BseNI | ACTGG | 7 cut(s) | 1719, 3646, 4083, 4823, 4895, 5026, 5064 |
| BseRI | GAGGAG | 7 cut(s) | 1412, 1475, 1561, 1782, 2914, 4689, 5961 |
| BseSI | GKGCMC | 3 cut(s) | 1010, 2119, 4426 |
| BseYI | CCCAGC | 4 cut(s) | 387, 2525, 2616, 4859 |
| BsgI | GTGCAG | 2 cut(s) | 2105, 4050 |
| Bsh1236I | CGCG | 7 cut(s) | 13, 1014, 2597, 2632, 3461, 3905, 6242 |
| Bsh1285I | CGRYCG | 3 cut(s) | 4045, 5606, 5837 |
| BshNI | GGYRCC | 4 cut(s) | 1738, 5188, 5869, 5906 |
| BshTI | ACCGGT | 1 cut(s) | 6158 |
| BshVI | ATCGAT | 4 cut(s) | 3636, 3752, 5602, 5667 |
| BsiEI | CGRYCG | 3 cut(s) | 4045, 5606, 5837 |
| BsiHKAI | GWGCWC | 7 cut(s) | 1010, 1382, 2119, 3533, 3766, 4426, 6311 |
| BsiHKCI | CYCGRG | 3 cut(s) | 4608, 4660, 6203 |
| BsiWI | CGTACG | 1 cut(s) | 5270 |
| BslFI | GGGAC | 3 cut(s) | 509, 3892, 4448 |
| BsmBI | CGTCTC | 3 cut(s) | 1787, 5705, 5768 |
| BsmFI | GGGAC | 3 cut(s) | 509, 3892, 4448 |
| BsmI | GAATGC | 2 cut(s) | 6300, 6320 |
| Bso31I | GGTCTC | 2 cut(s) | 2825, 4827 |
| BsoBI | CYCGRG | 3 cut(s) | 4608, 4660, 6203 |
| Bsp119I | TTCGAA | 4 cut(s) | 1707, 3009, 3416, 5320 |
| Bsp13I | TCCGGA | 1 cut(s) | 5249 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1722, 5609 |
| Bsp19I | CCATGG | 5 cut(s) | 1749, 3097, 4154, 5628, 5865 |
| Bsp68I | TCGCGA | 1 cut(s) | 2597 |
| BspCNI | CTCAG | 6 cut(s) | 1212, 1220, 1735, 3797, 3886, 6124 |
| BspDI | ATCGAT | 4 cut(s) | 3636, 3752, 5602, 5667 |
| BspEI | TCCGGA | 1 cut(s) | 5249 |
| BspFNI | CGCG | 7 cut(s) | 13, 1014, 2597, 2632, 3461, 3905, 6242 |
| BspHI | TCATGA | 1 cut(s) | 4848 |
| BspMAI | CTGCAG | 3 cut(s) | 4111, 4477, 5765 |
| BspMI | ACCTGC | 5 cut(s) | 2411, 2759, 4100, 4319, 4480 |
| BspPI | GGATC | 9 cut(s) | 2036, 2049, 2221, 3340, 4334, 5049, 5553, 5878, 5891 |
| BspT104I | TTCGAA | 4 cut(s) | 1707, 3009, 3416, 5320 |
| BspT107I | GGYRCC | 4 cut(s) | 1738, 5188, 5869, 5906 |
| BspTNI | GGTCTC | 2 cut(s) | 2825, 4827 |
| BsrBI | CCGCTC | 1 cut(s) | 1721 |
| BsrDI | GCAATG | 2 cut(s) | 3622, 6325 |
| BsrFI | RCCGGY | 2 cut(s) | 1316, 6158 |
| BsrI | ACTGG | 7 cut(s) | 1719, 3646, 4083, 4823, 4895, 5026, 5064 |
| BssAI | RCCGGY | 2 cut(s) | 1316, 6158 |
| BssNAI | GTATAC | 1 cut(s) | 3325 |
| BssNI | GRCGYC | 2 cut(s) | 1812, 5870 |
| BssSI | CACGAG | 3 cut(s) | 1516, 4539, 5935 |
| BssT1I | CCWWGG | 8 cut(s) | 1749, 2603, 3097, 3112, 4154, 5037, 5628, 5865 |
| Bst1107I | GTATAC | 1 cut(s) | 3325 |
| Bst2BI | CACGAG | 3 cut(s) | 1516, 4539, 5935 |
| Bst2UI | CCWGG | 4 cut(s) | 1480, 5657, 5675, 5904 |
| Bst6I | CTCTTC | 7 cut(s) | 652, 1368, 1530, 2585, 4609, 5248, 6074 |
| BstACI | GRCGYC | 2 cut(s) | 1812, 5870 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 4421 |
| BstBAI | YACGTR | 2 cut(s) | 5270, 5274 |
| BstBI | TTCGAA | 4 cut(s) | 1707, 3009, 3416, 5320 |
| BstC8I | GCNNGC | 6 cut(s) | 1387, 1695, 2315, 3495, 4866, 5728 |
| BstDSI | CCRYGG | 6 cut(s) | 1749, 2083, 3097, 4154, 5628, 5865 |
| BstFNI | CGCG | 7 cut(s) | 13, 1014, 2597, 2632, 3461, 3905, 6242 |
| BstH2I | RGCGCY | 1 cut(s) | 5873 |
| BstHHI | GCGC | 7 cut(s) | 857, 2494, 2756, 3947, 4415, 5872, 6244 |
| BstMCI | CGRYCG | 3 cut(s) | 4045, 5606, 5837 |
| BstNI | CCWGG | 4 cut(s) | 1480, 5657, 5675, 5904 |
| BstNSI | RCATGY | 8 cut(s) | 154, 585, 943, 2309, 2317, 4733, 4778, 5617 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 4232 |
| BstSFI | CTRYAG | 7 cut(s) | 329, 360, 1642, 4053, 4107, 4473, 5761 |
| BstSLI | GKGCMC | 3 cut(s) | 1010, 2119, 4426 |
| BstSNI | TACGTA | 1 cut(s) | 5274 |
| BstUI | CGCG | 7 cut(s) | 13, 1014, 2597, 2632, 3461, 3905, 6242 |
| BstV2I | GAAGAC | 2 cut(s) | 4725, 5076 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 1160, 2681, 3702 |
| BstZ17I | GTATAC | 1 cut(s) | 3325 |
| Bsu15I | ATCGAT | 4 cut(s) | 3636, 3752, 5602, 5667 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1207, 3342 |
| BsuI | GTATCC | 1 cut(s) | 3159 |
| BsuTUI | ATCGAT | 4 cut(s) | 3636, 3752, 5602, 5667 |
| BtgI | CCRYGG | 6 cut(s) | 1749, 2083, 3097, 4154, 5628, 5865 |
| BtgZI | GCGATG | 2 cut(s) | 2578, 3687 |
| BtsI | GCAGTG | 4 cut(s) | 650, 1500, 2117, 6265 |
| BtuMI | TCGCGA | 1 cut(s) | 2597 |
| BveI | ACCTGC | 5 cut(s) | 2411, 2759, 4100, 4319, 4480 |
| Cac8I | GCNNGC | 6 cut(s) | 1387, 1695, 2315, 3495, 4866, 5728 |
| CaiI | CAGNNNCTG | 2 cut(s) | 2205, 6357 |
| CciI | TCATGA | 1 cut(s) | 4848 |
| CfoI | GCGC | 7 cut(s) | 857, 2494, 2756, 3947, 4415, 5872, 6244 |
| Cfr10I | RCCGGY | 2 cut(s) | 1316, 6158 |
| Cfr9I | CCCGGG | 2 cut(s) | 4660, 6203 |
| ClaI | ATCGAT | 4 cut(s) | 3636, 3752, 5602, 5667 |
| CpoI | CGGWCCG | 1 cut(s) | 5721 |
| CseI | GACGC | 8 cut(s) | 12, 943, 1801, 1925, 2638, 4237, 4290, 5274 |
| CspAI | ACCGGT | 1 cut(s) | 6158 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1273, 1308 |
| CspI | CGGWCCG | 1 cut(s) | 5721 |
| DinI | GGCGCC | 1 cut(s) | 5871 |
| DraI | TTTAAA | 4 cut(s) | 568, 762, 1608, 4944 |
| DraIII | CACNNNGTG | 1 cut(s) | 4430 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 5719 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 5719 |
| EaeI | YGGCCR | 2 cut(s) | 1716, 3109 |
| Eam1104I | CTCTTC | 7 cut(s) | 652, 1368, 1530, 2585, 4609, 5248, 6074 |
| EarI | CTCTTC | 7 cut(s) | 652, 1368, 1530, 2585, 4609, 5248, 6074 |
| EciI | GGCGGA | 2 cut(s) | 5558, 5713 |
| Ecl136II | GAGCTC | 2 cut(s) | 1380, 3764 |
| Eco105I | TACGTA | 1 cut(s) | 5274 |
| Eco130I | CCWWGG | 8 cut(s) | 1749, 2603, 3097, 3112, 4154, 5037, 5628, 5865 |
| Eco147I | AGGCCT | 1 cut(s) | 5660 |
| Eco24I | GRGCYC | 5 cut(s) | 386, 1305, 1382, 3766, 4136 |
| Eco31I | GGTCTC | 2 cut(s) | 2825, 4827 |
| Eco47I | GGWCC | 3 cut(s) | 496, 3686, 5721 |
| Eco53kI | GAGCTC | 2 cut(s) | 1380, 3764 |
| Eco57I | CTGAAG | 2 cut(s) | 1293, 3219 |
| Eco81I | CCTNAGG | 2 cut(s) | 1207, 3342 |
| Eco88I | CYCGRG | 3 cut(s) | 4608, 4660, 6203 |
| EcoICRI | GAGCTC | 2 cut(s) | 1380, 3764 |
| EcoO109I | RGGNCCY | 2 cut(s) | 496, 4613 |
| EcoRI | GAATTC | 6 cut(s) | 1680, 1956, 2908, 3058, 3566, 4240 |
| EcoRII | CCWGG | 4 cut(s) | 1478, 5655, 5673, 5902 |
| EcoT14I | CCWWGG | 8 cut(s) | 1749, 2603, 3097, 3112, 4154, 5037, 5628, 5865 |
| EcoT22I | ATGCAT | 1 cut(s) | 2319 |
| EcoT38I | GRGCYC | 5 cut(s) | 386, 1305, 1382, 3766, 4136 |
| EgeI | GGCGCC | 1 cut(s) | 5871 |
| EheI | GGCGCC | 1 cut(s) | 5871 |
| ErhI | CCWWGG | 8 cut(s) | 1749, 2603, 3097, 3112, 4154, 5037, 5628, 5865 |
| Esp3I | CGTCTC | 3 cut(s) | 1787, 5705, 5768 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 179, 211, 1887, 1919 |
| FaqI | GGGAC | 3 cut(s) | 509, 3892, 4448 |
| FauI | CCCGC | 4 cut(s) | 2104, 2771, 5563, 6218 |
| FauNDI | CATATG | 2 cut(s) | 2352, 4909 |
| FbaI | TGATCA | 3 cut(s) | 1018, 2718, 6138 |
| FblI | GTMKAC | 1 cut(s) | 3324 |
| FriOI | GRGCYC | 5 cut(s) | 386, 1305, 1382, 3766, 4136 |
| GlaI | GCGC | 7 cut(s) | 856, 2493, 2755, 3946, 4414, 5871, 6243 |
| GsaI | CCCAGC | 4 cut(s) | 391, 2529, 2620, 4863 |
| GsuI | CTGGAG | 3 cut(s) | 2477, 4100, 4809 |
| HaeII | RGCGCY | 1 cut(s) | 5873 |
| HgaI | GACGC | 8 cut(s) | 12, 943, 1801, 1925, 2638, 4237, 4290, 5274 |
| HhaI | GCGC | 7 cut(s) | 857, 2494, 2756, 3947, 4415, 5872, 6244 |
| Hin1I | GRCGYC | 2 cut(s) | 1812, 5870 |
| Hin6I | GCGC | 7 cut(s) | 855, 2492, 2754, 3945, 4413, 5870, 6242 |
| HinP1I | GCGC | 7 cut(s) | 855, 2492, 2754, 3945, 4413, 5870, 6242 |
| HincII | GTYRAC | 6 cut(s) | 415, 579, 3192, 3997, 4522, 5176 |
| HindII | GTYRAC | 6 cut(s) | 415, 579, 3192, 3997, 4522, 5176 |
| HindIII | AAGCTT | 2 cut(s) | 3445, 4938 |
| HpaI | GTTAAC | 2 cut(s) | 415, 5176 |
| Hpy99I | CGWCG | 9 cut(s) | 28, 1817, 2632, 4194, 4407, 4883, 5290, 5807, 5842 |
| HpyCH4IV | ACGT | 8 cut(s) | 1125, 1975, 2225, 3774, 4503, 4518, 5269, 5273 |
| HpySE526I | ACGT | 8 cut(s) | 1125, 1975, 2225, 3774, 4503, 4518, 5269, 5273 |
| Hsp92I | GRCGYC | 2 cut(s) | 1812, 5870 |
| HspAI | GCGC | 7 cut(s) | 855, 2492, 2754, 3945, 4413, 5870, 6242 |
| KasI | GGCGCC | 1 cut(s) | 5869 |
| Kpn2I | TCCGGA | 1 cut(s) | 5249 |
| Ksp22I | TGATCA | 3 cut(s) | 1018, 2718, 6138 |
| KspAI | GTTAAC | 2 cut(s) | 415, 5176 |
| MaeII | ACGT | 8 cut(s) | 1125, 1975, 2225, 3774, 4503, 4518, 5269, 5273 |
| MbiI | CCGCTC | 1 cut(s) | 1721 |
| MfeI | CAATTG | 1 cut(s) | 4014 |
| MlsI | TGGCCA | 1 cut(s) | 3111 |
| MluI | ACGCGT | 2 cut(s) | 11, 1012 |
| MluNI | TGGCCA | 1 cut(s) | 3111 |
| Mly113I | GGCGCC | 1 cut(s) | 5870 |
| MlyI | GAGTC | 7 cut(s) | 1356, 2265, 3208, 4416, 5090, 5334, 5816 |
| Mox20I | TGGCCA | 1 cut(s) | 3111 |
| Mph1103I | ATGCAT | 1 cut(s) | 2319 |
| MroI | TCCGGA | 1 cut(s) | 5249 |
| MroXI | GAANNNNTTC | 4 cut(s) | 67, 191, 3005, 3062 |
| MscI | TGGCCA | 1 cut(s) | 3111 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1944, 1967, 2693, 5183, 6032 |
| Msp20I | TGGCCA | 1 cut(s) | 3111 |
| MspA1I | CMGCKG | 4 cut(s) | 1726, 2529, 4829, 6148 |
| MunI | CAATTG | 1 cut(s) | 4014 |
| Mva1269I | GAATGC | 2 cut(s) | 6300, 6320 |
| MvaI | CCWGG | 4 cut(s) | 1480, 5657, 5675, 5904 |
| MvnI | CGCG | 7 cut(s) | 13, 1014, 2597, 2632, 3461, 3905, 6242 |
| NarI | GGCGCC | 1 cut(s) | 5870 |
| NcoI | CCATGG | 5 cut(s) | 1749, 3097, 4154, 5628, 5865 |
| NdeI | CATATG | 2 cut(s) | 2352, 4909 |
| NmeAIII | GCCGAG | 3 cut(s) | 1085, 1361, 2531 |
| NmuCI | GTSAC | 4 cut(s) | 2139, 2759, 5239, 5790 |
| NruI | TCGCGA | 1 cut(s) | 2597 |
| NsiI | ATGCAT | 1 cut(s) | 2319 |
| NspI | RCATGY | 8 cut(s) | 154, 585, 943, 2309, 2317, 4733, 4778, 5617 |
| NspV | TTCGAA | 4 cut(s) | 1707, 3009, 3416, 5320 |
| OliI | CACNNNNGTG | 1 cut(s) | 2693 |
| PaeI | GCATGC | 1 cut(s) | 2317 |
| PagI | TCATGA | 1 cut(s) | 4848 |
| PaqCI | CACCTGC | 1 cut(s) | 2759 |
| PceI | AGGCCT | 1 cut(s) | 5660 |
| PciI | ACATGT | 1 cut(s) | 581 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1161, 2564 |
| PctI | GAATGC | 2 cut(s) | 6300, 6320 |
| PdmI | GAANNNNTTC | 4 cut(s) | 67, 191, 3005, 3062 |
| Pfl23II | CGTACG | 1 cut(s) | 5270 |
| PflFI | GACNNNGTC | 1 cut(s) | 4505 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 4078 |
| PfoI | TCCNGGA | 1 cut(s) | 1416 |
| PinAI | ACCGGT | 1 cut(s) | 6158 |
| Ple19I | CGATCG | 2 cut(s) | 4045, 5606 |
| PleI | GAGTC | 7 cut(s) | 1356, 2264, 3208, 4415, 5089, 5334, 5815 |
| PluTI | GGCGCC | 1 cut(s) | 5873 |
| PpsI | GAGTC | 7 cut(s) | 1356, 2264, 3208, 4415, 5089, 5334, 5815 |
| Ppu21I | YACGTR | 2 cut(s) | 5270, 5274 |
| PpuMI | RGGWCCY | 1 cut(s) | 496 |
| PscI | ACATGT | 1 cut(s) | 581 |
| PshAI | GACNNNNGTC | 1 cut(s) | 4232 |
| PshBI | ATTAAT | 4 cut(s) | 176, 1557, 2868, 3086 |
| PsiI | TTATAA | 3 cut(s) | 1584, 2843, 6107 |
| Psp124BI | GAGCTC | 2 cut(s) | 1382, 3766 |
| Psp1406I | AACGTT | 1 cut(s) | 1975 |
| Psp5II | RGGWCCY | 1 cut(s) | 496 |
| Psp6I | CCWGG | 4 cut(s) | 1478, 5655, 5673, 5902 |
| PspFI | CCCAGC | 4 cut(s) | 387, 2525, 2616, 4859 |
| PspGI | CCWGG | 4 cut(s) | 1478, 5655, 5673, 5902 |
| PspLI | CGTACG | 1 cut(s) | 5270 |
| PspPPI | RGGWCCY | 1 cut(s) | 496 |
| PstI | CTGCAG | 3 cut(s) | 4111, 4477, 5765 |
| PstNI | CAGNNNCTG | 2 cut(s) | 2205, 6357 |
| PsyI | GACNNNGTC | 1 cut(s) | 4505 |
| PvuI | CGATCG | 2 cut(s) | 4045, 5606 |
| PvuII | CAGCTG | 3 cut(s) | 1726, 4829, 6148 |
| RruI | TCGCGA | 1 cut(s) | 2597 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1944, 1967, 2693, 5183, 6032 |
| Rsr2I | CGGWCCG | 1 cut(s) | 5721 |
| RsrII | CGGWCCG | 1 cut(s) | 5721 |
| SacI | GAGCTC | 2 cut(s) | 1382, 3766 |
| ScaI | AGTACT | 1 cut(s) | 1841 |
| SchI | GAGTC | 7 cut(s) | 1356, 2265, 3208, 4416, 5090, 5334, 5816 |
| SfcI | CTRYAG | 7 cut(s) | 329, 360, 1642, 4053, 4107, 4473, 5761 |
| SfoI | GGCGCC | 1 cut(s) | 5871 |
| SfuI | TTCGAA | 4 cut(s) | 1707, 3009, 3416, 5320 |
| SinI | GGWCC | 3 cut(s) | 496, 3686, 5721 |
| SmaI | CCCGGG | 2 cut(s) | 4662, 6205 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1944, 1967, 2693, 5183, 6032 |
| SmlI | CTYRAG | 5 cut(s) | 1982, 2020, 2263, 5193, 6299 |
| SmoI | CTYRAG | 5 cut(s) | 1982, 2020, 2263, 5193, 6299 |
| SnaBI | TACGTA | 1 cut(s) | 5274 |
| SphI | GCATGC | 1 cut(s) | 2317 |
| SseBI | AGGCCT | 1 cut(s) | 5660 |
| SspDI | GGCGCC | 1 cut(s) | 5869 |
| SspI | AATATT | 5 cut(s) | 698, 1561, 2001, 2960, 3138 |
| SstI | GAGCTC | 2 cut(s) | 1382, 3766 |
| StuI | AGGCCT | 1 cut(s) | 5660 |
| StyI | CCWWGG | 8 cut(s) | 1749, 2603, 3097, 3112, 4154, 5037, 5628, 5865 |
| TaiI | ACGT | 8 cut(s) | 1128, 1978, 2228, 3777, 4506, 4521, 5272, 5276 |
| TaqII | GACCGA | 1 cut(s) | 3492 |
| TatI | WGTACW | 4 cut(s) | 1111, 1839, 2422, 4022 |
| TauI | GCSGC | 3 cut(s) | 1721, 3464, 4583 |
| TseFI | GTSAC | 4 cut(s) | 2139, 2759, 5239, 5790 |
| Tsp45I | GTSAC | 4 cut(s) | 2139, 2759, 5239, 5790 |
| TspMI | CCCGGG | 2 cut(s) | 4660, 6203 |
| Tth111I | GACNNNGTC | 1 cut(s) | 4505 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 4078 |
| VneI | GTGCAC | 3 cut(s) | 1006, 2115, 4422 |
| VpaK11BI | GGWCC | 3 cut(s) | 496, 3686, 5721 |
| VspI | ATTAAT | 4 cut(s) | 176, 1557, 2868, 3086 |
| XceI | RCATGY | 8 cut(s) | 154, 585, 943, 2309, 2317, 4733, 4778, 5617 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 3104 |
| XmaI | CCCGGG | 2 cut(s) | 4660, 6203 |
| XmaJI | CCTAGG | 1 cut(s) | 2603 |
| XmiI | GTMKAC | 1 cut(s) | 3324 |
| XmnI | GAANNNNTTC | 4 cut(s) | 67, 191, 3005, 3062 |
| ZrmI | AGTACT | 1 cut(s) | 1841 |
| Zsp2I | ATGCAT | 1 cut(s) | 2319 |